A low-cost benthic incubation chamber for in-situ community metabolism measurements

Benthic incubation chambers facilitate in-situ metabolism studies in shallow water environments. They are used to isolate the water surrounding a study organism or community so that changes in water chemistry can be quantified to characterise physiological processes such as photosynthesis, respiration, and calcification. Such field measurements capture the biological processes taking place within the benthic community while incorporating the influence of environmental variables that are often difficult to recreate in ex-situ settings. Variations in benthic chamber designs have evolved for a range of applications. In this study, we built upon previous designs to create a novel chamber, which is (1) low-cost and assembled without specialised equipment, (2) easily reproducible, (3) minimally invasive, (4) adaptable to varied substrates, and (5) comparable with other available designs in performance. We tested the design in the laboratory and field and found that it achieved the outlined objectives. Using non-specialised materials, we were able to construct the chamber at a low cost (under $20 USD per unit), while maintaining similar performance and reproducibility with that of existing designs. Laboratory and field tests demonstrated minimal leakage (2.08 ± 0.78% water exchange over 4 h) and acceptable light transmission (86.9 ± 1.9%), results comparable to those reported for other chambers. In the field, chambers were deployed in a shallow coastal environment in Akumal, Mexico, to measure productivity of seagrass, and coral-, algae-, and sand-dominated reef patches. In both case studies, production rates aligned with those of comparable benthic chamber deployments in the literature and followed established trends with light, the primary driver of benthic metabolism, indicating robust performance under field conditions. We demonstrate that our low-cost benthic chamber design uses locally accessible and minimal resources, is adaptable for a variety of field settings, and can be used to collect reliable and repeatable benthic metabolism data. This chamber has the potential to broaden accessibility and applications of in-situ incubations for future studies. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Jennifer Mallon [verfasserIn] Anastazia T. Banaszak [verfasserIn] Lauren Donachie [verfasserIn] Dan Exton [verfasserIn] Tyler Cyronak [verfasserIn] Thorsten Balke [verfasserIn] Adrian M. Bass [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	In-situ metabolism Low-cost chamber Coral reefs Production Seagrass Sediments

Übergeordnetes Werk:	In: PeerJ - PeerJ Inc., 2013, 10, p e13116(2022)
Übergeordnetes Werk:	volume:10, p e13116 ; year:2022

Links:	Link aufrufen Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.7717/peerj.13116

Katalog-ID:	DOAJ026335247

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allfieldsGer	10.7717/peerj.13116 doi (DE-627)DOAJ026335247 (DE-599)DOAJ938571754fb3419386647c41605c8ae7 DE-627 ger DE-627 rakwb eng QH301-705.5 Jennifer Mallon verfasserin aut A low-cost benthic incubation chamber for in-situ community metabolism measurements 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Benthic incubation chambers facilitate in-situ metabolism studies in shallow water environments. They are used to isolate the water surrounding a study organism or community so that changes in water chemistry can be quantified to characterise physiological processes such as photosynthesis, respiration, and calcification. Such field measurements capture the biological processes taking place within the benthic community while incorporating the influence of environmental variables that are often difficult to recreate in ex-situ settings. Variations in benthic chamber designs have evolved for a range of applications. In this study, we built upon previous designs to create a novel chamber, which is (1) low-cost and assembled without specialised equipment, (2) easily reproducible, (3) minimally invasive, (4) adaptable to varied substrates, and (5) comparable with other available designs in performance. We tested the design in the laboratory and field and found that it achieved the outlined objectives. Using non-specialised materials, we were able to construct the chamber at a low cost (under $20 USD per unit), while maintaining similar performance and reproducibility with that of existing designs. Laboratory and field tests demonstrated minimal leakage (2.08 ± 0.78% water exchange over 4 h) and acceptable light transmission (86.9 ± 1.9%), results comparable to those reported for other chambers. In the field, chambers were deployed in a shallow coastal environment in Akumal, Mexico, to measure productivity of seagrass, and coral-, algae-, and sand-dominated reef patches. In both case studies, production rates aligned with those of comparable benthic chamber deployments in the literature and followed established trends with light, the primary driver of benthic metabolism, indicating robust performance under field conditions. We demonstrate that our low-cost benthic chamber design uses locally accessible and minimal resources, is adaptable for a variety of field settings, and can be used to collect reliable and repeatable benthic metabolism data. This chamber has the potential to broaden accessibility and applications of in-situ incubations for future studies. In-situ metabolism Low-cost chamber Coral reefs Production Seagrass Sediments Medicine R Biology (General) Anastazia T. Banaszak verfasserin aut Lauren Donachie verfasserin aut Dan Exton verfasserin aut Tyler Cyronak verfasserin aut Thorsten Balke verfasserin aut Adrian M. Bass verfasserin aut In PeerJ PeerJ Inc., 2013 10, p e13116(2022) (DE-627)736558624 (DE-600)2703241-3 21678359 nnns volume:10, p e13116 year:2022 https://doi.org/10.7717/peerj.13116 kostenfrei https://doaj.org/article/938571754fb3419386647c41605c8ae7 kostenfrei https://peerj.com/articles/13116.pdf kostenfrei https://peerj.com/articles/13116/ kostenfrei https://doaj.org/toc/2167-8359 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10, p e13116 2022
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authorswithroles_txt_mv	Jennifer Mallon @@aut@@ Anastazia T. Banaszak @@aut@@ Lauren Donachie @@aut@@ Dan Exton @@aut@@ Tyler Cyronak @@aut@@ Thorsten Balke @@aut@@ Adrian M. Bass @@aut@@
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author	Jennifer Mallon
spellingShingle	Jennifer Mallon misc QH301-705.5 misc In-situ metabolism misc Low-cost chamber misc Coral reefs misc Production misc Seagrass misc Sediments misc Medicine misc R misc Biology (General) A low-cost benthic incubation chamber for in-situ community metabolism measurements
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topic_title	QH301-705.5 A low-cost benthic incubation chamber for in-situ community metabolism measurements In-situ metabolism Low-cost chamber Coral reefs Production Seagrass Sediments
topic	misc QH301-705.5 misc In-situ metabolism misc Low-cost chamber misc Coral reefs misc Production misc Seagrass misc Sediments misc Medicine misc R misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc In-situ metabolism misc Low-cost chamber misc Coral reefs misc Production misc Seagrass misc Sediments misc Medicine misc R misc Biology (General)
topic_browse	misc QH301-705.5 misc In-situ metabolism misc Low-cost chamber misc Coral reefs misc Production misc Seagrass misc Sediments misc Medicine misc R misc Biology (General)
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
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title	A low-cost benthic incubation chamber for in-situ community metabolism measurements
ctrlnum	(DE-627)DOAJ026335247 (DE-599)DOAJ938571754fb3419386647c41605c8ae7
title_full	A low-cost benthic incubation chamber for in-situ community metabolism measurements
author_sort	Jennifer Mallon
journal	PeerJ
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author_browse	Jennifer Mallon Anastazia T. Banaszak Lauren Donachie Dan Exton Tyler Cyronak Thorsten Balke Adrian M. Bass
container_volume	10, p e13116
class	QH301-705.5
format_se	Elektronische Aufsätze
author-letter	Jennifer Mallon
doi_str_mv	10.7717/peerj.13116
author2-role	verfasserin
title_sort	low-cost benthic incubation chamber for in-situ community metabolism measurements
callnumber	QH301-705.5
title_auth	A low-cost benthic incubation chamber for in-situ community metabolism measurements
abstract	Benthic incubation chambers facilitate in-situ metabolism studies in shallow water environments. They are used to isolate the water surrounding a study organism or community so that changes in water chemistry can be quantified to characterise physiological processes such as photosynthesis, respiration, and calcification. Such field measurements capture the biological processes taking place within the benthic community while incorporating the influence of environmental variables that are often difficult to recreate in ex-situ settings. Variations in benthic chamber designs have evolved for a range of applications. In this study, we built upon previous designs to create a novel chamber, which is (1) low-cost and assembled without specialised equipment, (2) easily reproducible, (3) minimally invasive, (4) adaptable to varied substrates, and (5) comparable with other available designs in performance. We tested the design in the laboratory and field and found that it achieved the outlined objectives. Using non-specialised materials, we were able to construct the chamber at a low cost (under $20 USD per unit), while maintaining similar performance and reproducibility with that of existing designs. Laboratory and field tests demonstrated minimal leakage (2.08 ± 0.78% water exchange over 4 h) and acceptable light transmission (86.9 ± 1.9%), results comparable to those reported for other chambers. In the field, chambers were deployed in a shallow coastal environment in Akumal, Mexico, to measure productivity of seagrass, and coral-, algae-, and sand-dominated reef patches. In both case studies, production rates aligned with those of comparable benthic chamber deployments in the literature and followed established trends with light, the primary driver of benthic metabolism, indicating robust performance under field conditions. We demonstrate that our low-cost benthic chamber design uses locally accessible and minimal resources, is adaptable for a variety of field settings, and can be used to collect reliable and repeatable benthic metabolism data. This chamber has the potential to broaden accessibility and applications of in-situ incubations for future studies.
abstractGer	Benthic incubation chambers facilitate in-situ metabolism studies in shallow water environments. They are used to isolate the water surrounding a study organism or community so that changes in water chemistry can be quantified to characterise physiological processes such as photosynthesis, respiration, and calcification. Such field measurements capture the biological processes taking place within the benthic community while incorporating the influence of environmental variables that are often difficult to recreate in ex-situ settings. Variations in benthic chamber designs have evolved for a range of applications. In this study, we built upon previous designs to create a novel chamber, which is (1) low-cost and assembled without specialised equipment, (2) easily reproducible, (3) minimally invasive, (4) adaptable to varied substrates, and (5) comparable with other available designs in performance. We tested the design in the laboratory and field and found that it achieved the outlined objectives. Using non-specialised materials, we were able to construct the chamber at a low cost (under $20 USD per unit), while maintaining similar performance and reproducibility with that of existing designs. Laboratory and field tests demonstrated minimal leakage (2.08 ± 0.78% water exchange over 4 h) and acceptable light transmission (86.9 ± 1.9%), results comparable to those reported for other chambers. In the field, chambers were deployed in a shallow coastal environment in Akumal, Mexico, to measure productivity of seagrass, and coral-, algae-, and sand-dominated reef patches. In both case studies, production rates aligned with those of comparable benthic chamber deployments in the literature and followed established trends with light, the primary driver of benthic metabolism, indicating robust performance under field conditions. We demonstrate that our low-cost benthic chamber design uses locally accessible and minimal resources, is adaptable for a variety of field settings, and can be used to collect reliable and repeatable benthic metabolism data. This chamber has the potential to broaden accessibility and applications of in-situ incubations for future studies.
abstract_unstemmed	Benthic incubation chambers facilitate in-situ metabolism studies in shallow water environments. They are used to isolate the water surrounding a study organism or community so that changes in water chemistry can be quantified to characterise physiological processes such as photosynthesis, respiration, and calcification. Such field measurements capture the biological processes taking place within the benthic community while incorporating the influence of environmental variables that are often difficult to recreate in ex-situ settings. Variations in benthic chamber designs have evolved for a range of applications. In this study, we built upon previous designs to create a novel chamber, which is (1) low-cost and assembled without specialised equipment, (2) easily reproducible, (3) minimally invasive, (4) adaptable to varied substrates, and (5) comparable with other available designs in performance. We tested the design in the laboratory and field and found that it achieved the outlined objectives. Using non-specialised materials, we were able to construct the chamber at a low cost (under $20 USD per unit), while maintaining similar performance and reproducibility with that of existing designs. Laboratory and field tests demonstrated minimal leakage (2.08 ± 0.78% water exchange over 4 h) and acceptable light transmission (86.9 ± 1.9%), results comparable to those reported for other chambers. In the field, chambers were deployed in a shallow coastal environment in Akumal, Mexico, to measure productivity of seagrass, and coral-, algae-, and sand-dominated reef patches. In both case studies, production rates aligned with those of comparable benthic chamber deployments in the literature and followed established trends with light, the primary driver of benthic metabolism, indicating robust performance under field conditions. We demonstrate that our low-cost benthic chamber design uses locally accessible and minimal resources, is adaptable for a variety of field settings, and can be used to collect reliable and repeatable benthic metabolism data. This chamber has the potential to broaden accessibility and applications of in-situ incubations for future studies.
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title_short	A low-cost benthic incubation chamber for in-situ community metabolism measurements
url	https://doi.org/10.7717/peerj.13116 https://doaj.org/article/938571754fb3419386647c41605c8ae7 https://peerj.com/articles/13116.pdf https://peerj.com/articles/13116/ https://doaj.org/toc/2167-8359
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author2	Anastazia T. Banaszak Lauren Donachie Dan Exton Tyler Cyronak Thorsten Balke Adrian M. Bass
author2Str	Anastazia T. Banaszak Lauren Donachie Dan Exton Tyler Cyronak Thorsten Balke Adrian M. Bass
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up_date	2024-07-03T20:23:43.723Z
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