Identification of Aberrantly-Expressed Long Non-Coding RNAs in Osteoblastic Cells from Osteoporotic Patients

Osteoporosis (OP) is a multifactorial disease influenced by genetic, epigenetic, and environmental factors. One of the main causes of the bone homeostasis alteration is inflammation resulting in excessive bone resorption. Long non-coding RNAs (lncRNAs), have a crucial role in regulating many importa...
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Autor*in:	Federica Centofanti [verfasserIn] Massimo Santoro [verfasserIn] Mario Marini [verfasserIn] Virginia Veronica Visconti [verfasserIn] Anna Maria Rinaldi [verfasserIn] Monica Celi [verfasserIn] Giovanna D’Arcangelo [verfasserIn] Giuseppe Novelli [verfasserIn] Augusto Orlandi [verfasserIn] Virginia Tancredi [verfasserIn] Umberto Tarantino [verfasserIn] Annalisa Botta [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	osteoporosis epigenetics long non-coding rnas inflammation bone homeostasis osteoblast

Übergeordnetes Werk:	In: Biomedicines - MDPI AG, 2014, 8(2020), 3, p 65
Übergeordnetes Werk:	volume:8 ; year:2020 ; number:3, p 65

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/biomedicines8030065

Katalog-ID:	DOAJ026956489

Internformat


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520			\|a Osteoporosis (OP) is a multifactorial disease influenced by genetic, epigenetic, and environmental factors. One of the main causes of the bone homeostasis alteration is inflammation resulting in excessive bone resorption. Long non-coding RNAs (lncRNAs), have a crucial role in regulating many important biological processes in bone, including inflammation. We designed our study to identify lncRNAs misregulated in osteoblast primary cultures derived from OP patients (<i<n</i< = 4), and controls (CTRs, <i<n</i< = 4) with the aim of predicting possible RNA and/or protein targets implicated in this multifactorial disease. We focused on 84 lncRNAs regulating the expression of pro-inflammatory and anti-inflammatory genes and miRNAs. In silico analysis was utilized to predict the interaction of lncRNAs with miRNAs, mRNAs, and proteins targets. Six lncRNAs were significantly down-regulated in OP patients compared to controls: <i<CEP83-AS1</i<, <i<RP11-84C13.1</i<, <i<CTC-487M23.5</i<, <i<GAS5</i<, <i<NCBP2-AS2</i<, and <i<SDCBP2-AS1</i<. Bioinformatic analyses identified HDCA2, PTX3, and FGF2 proteins as downstream targets of <i<CTC-487M23.5, GAS5</i<, and <i<RP11-84C13.1</i< lncRNAs mediated by the interaction with miRNAs implicated in OP pathogenesis, including <i<miR-21-5</i<p. Altogether, these data open a new regulatory mechanism of gene expression in bone homeostasis and could direct the development of future therapeutic approaches.
650		4	\|a osteoporosis
650		4	\|a epigenetics
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653		0	\|a Biology (General)
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author_variant	f c fc m s ms m m mm v v v vvv a m r amr m c mc g d gd g n gn a o ao v t vt u t ut a b ab
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allfields	10.3390/biomedicines8030065 doi (DE-627)DOAJ026956489 (DE-599)DOAJ76e56cf2fc994f4bbb313ca45be30151 DE-627 ger DE-627 rakwb eng QH301-705.5 Federica Centofanti verfasserin aut Identification of Aberrantly-Expressed Long Non-Coding RNAs in Osteoblastic Cells from Osteoporotic Patients 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Osteoporosis (OP) is a multifactorial disease influenced by genetic, epigenetic, and environmental factors. One of the main causes of the bone homeostasis alteration is inflammation resulting in excessive bone resorption. Long non-coding RNAs (lncRNAs), have a crucial role in regulating many important biological processes in bone, including inflammation. We designed our study to identify lncRNAs misregulated in osteoblast primary cultures derived from OP patients (<i<n</i< = 4), and controls (CTRs, <i<n</i< = 4) with the aim of predicting possible RNA and/or protein targets implicated in this multifactorial disease. We focused on 84 lncRNAs regulating the expression of pro-inflammatory and anti-inflammatory genes and miRNAs. In silico analysis was utilized to predict the interaction of lncRNAs with miRNAs, mRNAs, and proteins targets. Six lncRNAs were significantly down-regulated in OP patients compared to controls: <i<CEP83-AS1</i<, <i<RP11-84C13.1</i<, <i<CTC-487M23.5</i<, <i<GAS5</i<, <i<NCBP2-AS2</i<, and <i<SDCBP2-AS1</i<. Bioinformatic analyses identified HDCA2, PTX3, and FGF2 proteins as downstream targets of <i<CTC-487M23.5, GAS5</i<, and <i<RP11-84C13.1</i< lncRNAs mediated by the interaction with miRNAs implicated in OP pathogenesis, including <i<miR-21-5</i<p. Altogether, these data open a new regulatory mechanism of gene expression in bone homeostasis and could direct the development of future therapeutic approaches. osteoporosis epigenetics long non-coding rnas inflammation bone homeostasis osteoblast Biology (General) Massimo Santoro verfasserin aut Mario Marini verfasserin aut Virginia Veronica Visconti verfasserin aut Anna Maria Rinaldi verfasserin aut Monica Celi verfasserin aut Giovanna D’Arcangelo verfasserin aut Giuseppe Novelli verfasserin aut Augusto Orlandi verfasserin aut Virginia Tancredi verfasserin aut Umberto Tarantino verfasserin aut Annalisa Botta verfasserin aut In Biomedicines MDPI AG, 2014 8(2020), 3, p 65 (DE-627)750370483 (DE-600)2720867-9 22279059 nnns volume:8 year:2020 number:3, p 65 https://doi.org/10.3390/biomedicines8030065 kostenfrei https://doaj.org/article/76e56cf2fc994f4bbb313ca45be30151 kostenfrei https://www.mdpi.com/2227-9059/8/3/65 kostenfrei https://doaj.org/toc/2227-9059 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 3, p 65
spelling	10.3390/biomedicines8030065 doi (DE-627)DOAJ026956489 (DE-599)DOAJ76e56cf2fc994f4bbb313ca45be30151 DE-627 ger DE-627 rakwb eng QH301-705.5 Federica Centofanti verfasserin aut Identification of Aberrantly-Expressed Long Non-Coding RNAs in Osteoblastic Cells from Osteoporotic Patients 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Osteoporosis (OP) is a multifactorial disease influenced by genetic, epigenetic, and environmental factors. One of the main causes of the bone homeostasis alteration is inflammation resulting in excessive bone resorption. Long non-coding RNAs (lncRNAs), have a crucial role in regulating many important biological processes in bone, including inflammation. We designed our study to identify lncRNAs misregulated in osteoblast primary cultures derived from OP patients (<i<n</i< = 4), and controls (CTRs, <i<n</i< = 4) with the aim of predicting possible RNA and/or protein targets implicated in this multifactorial disease. We focused on 84 lncRNAs regulating the expression of pro-inflammatory and anti-inflammatory genes and miRNAs. In silico analysis was utilized to predict the interaction of lncRNAs with miRNAs, mRNAs, and proteins targets. Six lncRNAs were significantly down-regulated in OP patients compared to controls: <i<CEP83-AS1</i<, <i<RP11-84C13.1</i<, <i<CTC-487M23.5</i<, <i<GAS5</i<, <i<NCBP2-AS2</i<, and <i<SDCBP2-AS1</i<. Bioinformatic analyses identified HDCA2, PTX3, and FGF2 proteins as downstream targets of <i<CTC-487M23.5, GAS5</i<, and <i<RP11-84C13.1</i< lncRNAs mediated by the interaction with miRNAs implicated in OP pathogenesis, including <i<miR-21-5</i<p. Altogether, these data open a new regulatory mechanism of gene expression in bone homeostasis and could direct the development of future therapeutic approaches. osteoporosis epigenetics long non-coding rnas inflammation bone homeostasis osteoblast Biology (General) Massimo Santoro verfasserin aut Mario Marini verfasserin aut Virginia Veronica Visconti verfasserin aut Anna Maria Rinaldi verfasserin aut Monica Celi verfasserin aut Giovanna D’Arcangelo verfasserin aut Giuseppe Novelli verfasserin aut Augusto Orlandi verfasserin aut Virginia Tancredi verfasserin aut Umberto Tarantino verfasserin aut Annalisa Botta verfasserin aut In Biomedicines MDPI AG, 2014 8(2020), 3, p 65 (DE-627)750370483 (DE-600)2720867-9 22279059 nnns volume:8 year:2020 number:3, p 65 https://doi.org/10.3390/biomedicines8030065 kostenfrei https://doaj.org/article/76e56cf2fc994f4bbb313ca45be30151 kostenfrei https://www.mdpi.com/2227-9059/8/3/65 kostenfrei https://doaj.org/toc/2227-9059 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 3, p 65
allfields_unstemmed	10.3390/biomedicines8030065 doi (DE-627)DOAJ026956489 (DE-599)DOAJ76e56cf2fc994f4bbb313ca45be30151 DE-627 ger DE-627 rakwb eng QH301-705.5 Federica Centofanti verfasserin aut Identification of Aberrantly-Expressed Long Non-Coding RNAs in Osteoblastic Cells from Osteoporotic Patients 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Osteoporosis (OP) is a multifactorial disease influenced by genetic, epigenetic, and environmental factors. One of the main causes of the bone homeostasis alteration is inflammation resulting in excessive bone resorption. Long non-coding RNAs (lncRNAs), have a crucial role in regulating many important biological processes in bone, including inflammation. We designed our study to identify lncRNAs misregulated in osteoblast primary cultures derived from OP patients (<i<n</i< = 4), and controls (CTRs, <i<n</i< = 4) with the aim of predicting possible RNA and/or protein targets implicated in this multifactorial disease. We focused on 84 lncRNAs regulating the expression of pro-inflammatory and anti-inflammatory genes and miRNAs. In silico analysis was utilized to predict the interaction of lncRNAs with miRNAs, mRNAs, and proteins targets. Six lncRNAs were significantly down-regulated in OP patients compared to controls: <i<CEP83-AS1</i<, <i<RP11-84C13.1</i<, <i<CTC-487M23.5</i<, <i<GAS5</i<, <i<NCBP2-AS2</i<, and <i<SDCBP2-AS1</i<. Bioinformatic analyses identified HDCA2, PTX3, and FGF2 proteins as downstream targets of <i<CTC-487M23.5, GAS5</i<, and <i<RP11-84C13.1</i< lncRNAs mediated by the interaction with miRNAs implicated in OP pathogenesis, including <i<miR-21-5</i<p. Altogether, these data open a new regulatory mechanism of gene expression in bone homeostasis and could direct the development of future therapeutic approaches. osteoporosis epigenetics long non-coding rnas inflammation bone homeostasis osteoblast Biology (General) Massimo Santoro verfasserin aut Mario Marini verfasserin aut Virginia Veronica Visconti verfasserin aut Anna Maria Rinaldi verfasserin aut Monica Celi verfasserin aut Giovanna D’Arcangelo verfasserin aut Giuseppe Novelli verfasserin aut Augusto Orlandi verfasserin aut Virginia Tancredi verfasserin aut Umberto Tarantino verfasserin aut Annalisa Botta verfasserin aut In Biomedicines MDPI AG, 2014 8(2020), 3, p 65 (DE-627)750370483 (DE-600)2720867-9 22279059 nnns volume:8 year:2020 number:3, p 65 https://doi.org/10.3390/biomedicines8030065 kostenfrei https://doaj.org/article/76e56cf2fc994f4bbb313ca45be30151 kostenfrei https://www.mdpi.com/2227-9059/8/3/65 kostenfrei https://doaj.org/toc/2227-9059 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 3, p 65
allfieldsGer	10.3390/biomedicines8030065 doi (DE-627)DOAJ026956489 (DE-599)DOAJ76e56cf2fc994f4bbb313ca45be30151 DE-627 ger DE-627 rakwb eng QH301-705.5 Federica Centofanti verfasserin aut Identification of Aberrantly-Expressed Long Non-Coding RNAs in Osteoblastic Cells from Osteoporotic Patients 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Osteoporosis (OP) is a multifactorial disease influenced by genetic, epigenetic, and environmental factors. One of the main causes of the bone homeostasis alteration is inflammation resulting in excessive bone resorption. Long non-coding RNAs (lncRNAs), have a crucial role in regulating many important biological processes in bone, including inflammation. We designed our study to identify lncRNAs misregulated in osteoblast primary cultures derived from OP patients (<i<n</i< = 4), and controls (CTRs, <i<n</i< = 4) with the aim of predicting possible RNA and/or protein targets implicated in this multifactorial disease. We focused on 84 lncRNAs regulating the expression of pro-inflammatory and anti-inflammatory genes and miRNAs. In silico analysis was utilized to predict the interaction of lncRNAs with miRNAs, mRNAs, and proteins targets. Six lncRNAs were significantly down-regulated in OP patients compared to controls: <i<CEP83-AS1</i<, <i<RP11-84C13.1</i<, <i<CTC-487M23.5</i<, <i<GAS5</i<, <i<NCBP2-AS2</i<, and <i<SDCBP2-AS1</i<. Bioinformatic analyses identified HDCA2, PTX3, and FGF2 proteins as downstream targets of <i<CTC-487M23.5, GAS5</i<, and <i<RP11-84C13.1</i< lncRNAs mediated by the interaction with miRNAs implicated in OP pathogenesis, including <i<miR-21-5</i<p. Altogether, these data open a new regulatory mechanism of gene expression in bone homeostasis and could direct the development of future therapeutic approaches. osteoporosis epigenetics long non-coding rnas inflammation bone homeostasis osteoblast Biology (General) Massimo Santoro verfasserin aut Mario Marini verfasserin aut Virginia Veronica Visconti verfasserin aut Anna Maria Rinaldi verfasserin aut Monica Celi verfasserin aut Giovanna D’Arcangelo verfasserin aut Giuseppe Novelli verfasserin aut Augusto Orlandi verfasserin aut Virginia Tancredi verfasserin aut Umberto Tarantino verfasserin aut Annalisa Botta verfasserin aut In Biomedicines MDPI AG, 2014 8(2020), 3, p 65 (DE-627)750370483 (DE-600)2720867-9 22279059 nnns volume:8 year:2020 number:3, p 65 https://doi.org/10.3390/biomedicines8030065 kostenfrei https://doaj.org/article/76e56cf2fc994f4bbb313ca45be30151 kostenfrei https://www.mdpi.com/2227-9059/8/3/65 kostenfrei https://doaj.org/toc/2227-9059 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 3, p 65
allfieldsSound	10.3390/biomedicines8030065 doi (DE-627)DOAJ026956489 (DE-599)DOAJ76e56cf2fc994f4bbb313ca45be30151 DE-627 ger DE-627 rakwb eng QH301-705.5 Federica Centofanti verfasserin aut Identification of Aberrantly-Expressed Long Non-Coding RNAs in Osteoblastic Cells from Osteoporotic Patients 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Osteoporosis (OP) is a multifactorial disease influenced by genetic, epigenetic, and environmental factors. One of the main causes of the bone homeostasis alteration is inflammation resulting in excessive bone resorption. Long non-coding RNAs (lncRNAs), have a crucial role in regulating many important biological processes in bone, including inflammation. We designed our study to identify lncRNAs misregulated in osteoblast primary cultures derived from OP patients (<i<n</i< = 4), and controls (CTRs, <i<n</i< = 4) with the aim of predicting possible RNA and/or protein targets implicated in this multifactorial disease. We focused on 84 lncRNAs regulating the expression of pro-inflammatory and anti-inflammatory genes and miRNAs. In silico analysis was utilized to predict the interaction of lncRNAs with miRNAs, mRNAs, and proteins targets. Six lncRNAs were significantly down-regulated in OP patients compared to controls: <i<CEP83-AS1</i<, <i<RP11-84C13.1</i<, <i<CTC-487M23.5</i<, <i<GAS5</i<, <i<NCBP2-AS2</i<, and <i<SDCBP2-AS1</i<. Bioinformatic analyses identified HDCA2, PTX3, and FGF2 proteins as downstream targets of <i<CTC-487M23.5, GAS5</i<, and <i<RP11-84C13.1</i< lncRNAs mediated by the interaction with miRNAs implicated in OP pathogenesis, including <i<miR-21-5</i<p. Altogether, these data open a new regulatory mechanism of gene expression in bone homeostasis and could direct the development of future therapeutic approaches. osteoporosis epigenetics long non-coding rnas inflammation bone homeostasis osteoblast Biology (General) Massimo Santoro verfasserin aut Mario Marini verfasserin aut Virginia Veronica Visconti verfasserin aut Anna Maria Rinaldi verfasserin aut Monica Celi verfasserin aut Giovanna D’Arcangelo verfasserin aut Giuseppe Novelli verfasserin aut Augusto Orlandi verfasserin aut Virginia Tancredi verfasserin aut Umberto Tarantino verfasserin aut Annalisa Botta verfasserin aut In Biomedicines MDPI AG, 2014 8(2020), 3, p 65 (DE-627)750370483 (DE-600)2720867-9 22279059 nnns volume:8 year:2020 number:3, p 65 https://doi.org/10.3390/biomedicines8030065 kostenfrei https://doaj.org/article/76e56cf2fc994f4bbb313ca45be30151 kostenfrei https://www.mdpi.com/2227-9059/8/3/65 kostenfrei https://doaj.org/toc/2227-9059 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 3, p 65
language	English
source	In Biomedicines 8(2020), 3, p 65 volume:8 year:2020 number:3, p 65
sourceStr	In Biomedicines 8(2020), 3, p 65 volume:8 year:2020 number:3, p 65
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topic_facet	osteoporosis epigenetics long non-coding rnas inflammation bone homeostasis osteoblast Biology (General)
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container_title	Biomedicines
authorswithroles_txt_mv	Federica Centofanti @@aut@@ Massimo Santoro @@aut@@ Mario Marini @@aut@@ Virginia Veronica Visconti @@aut@@ Anna Maria Rinaldi @@aut@@ Monica Celi @@aut@@ Giovanna D’Arcangelo @@aut@@ Giuseppe Novelli @@aut@@ Augusto Orlandi @@aut@@ Virginia Tancredi @@aut@@ Umberto Tarantino @@aut@@ Annalisa Botta @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
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topic_title	QH301-705.5 Identification of Aberrantly-Expressed Long Non-Coding RNAs in Osteoblastic Cells from Osteoporotic Patients osteoporosis epigenetics long non-coding rnas inflammation bone homeostasis osteoblast
topic	misc QH301-705.5 misc osteoporosis misc epigenetics misc long non-coding rnas misc inflammation misc bone homeostasis misc osteoblast misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc osteoporosis misc epigenetics misc long non-coding rnas misc inflammation misc bone homeostasis misc osteoblast misc Biology (General)
topic_browse	misc QH301-705.5 misc osteoporosis misc epigenetics misc long non-coding rnas misc inflammation misc bone homeostasis misc osteoblast misc Biology (General)
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title	Identification of Aberrantly-Expressed Long Non-Coding RNAs in Osteoblastic Cells from Osteoporotic Patients
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title_full	Identification of Aberrantly-Expressed Long Non-Coding RNAs in Osteoblastic Cells from Osteoporotic Patients
author_sort	Federica Centofanti
journal	Biomedicines
journalStr	Biomedicines
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author_browse	Federica Centofanti Massimo Santoro Mario Marini Virginia Veronica Visconti Anna Maria Rinaldi Monica Celi Giovanna D’Arcangelo Giuseppe Novelli Augusto Orlandi Virginia Tancredi Umberto Tarantino Annalisa Botta
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author-letter	Federica Centofanti
doi_str_mv	10.3390/biomedicines8030065
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title_sort	identification of aberrantly-expressed long non-coding rnas in osteoblastic cells from osteoporotic patients
callnumber	QH301-705.5
title_auth	Identification of Aberrantly-Expressed Long Non-Coding RNAs in Osteoblastic Cells from Osteoporotic Patients
abstract	Osteoporosis (OP) is a multifactorial disease influenced by genetic, epigenetic, and environmental factors. One of the main causes of the bone homeostasis alteration is inflammation resulting in excessive bone resorption. Long non-coding RNAs (lncRNAs), have a crucial role in regulating many important biological processes in bone, including inflammation. We designed our study to identify lncRNAs misregulated in osteoblast primary cultures derived from OP patients (<i<n</i< = 4), and controls (CTRs, <i<n</i< = 4) with the aim of predicting possible RNA and/or protein targets implicated in this multifactorial disease. We focused on 84 lncRNAs regulating the expression of pro-inflammatory and anti-inflammatory genes and miRNAs. In silico analysis was utilized to predict the interaction of lncRNAs with miRNAs, mRNAs, and proteins targets. Six lncRNAs were significantly down-regulated in OP patients compared to controls: <i<CEP83-AS1</i<, <i<RP11-84C13.1</i<, <i<CTC-487M23.5</i<, <i<GAS5</i<, <i<NCBP2-AS2</i<, and <i<SDCBP2-AS1</i<. Bioinformatic analyses identified HDCA2, PTX3, and FGF2 proteins as downstream targets of <i<CTC-487M23.5, GAS5</i<, and <i<RP11-84C13.1</i< lncRNAs mediated by the interaction with miRNAs implicated in OP pathogenesis, including <i<miR-21-5</i<p. Altogether, these data open a new regulatory mechanism of gene expression in bone homeostasis and could direct the development of future therapeutic approaches.
abstractGer	Osteoporosis (OP) is a multifactorial disease influenced by genetic, epigenetic, and environmental factors. One of the main causes of the bone homeostasis alteration is inflammation resulting in excessive bone resorption. Long non-coding RNAs (lncRNAs), have a crucial role in regulating many important biological processes in bone, including inflammation. We designed our study to identify lncRNAs misregulated in osteoblast primary cultures derived from OP patients (<i<n</i< = 4), and controls (CTRs, <i<n</i< = 4) with the aim of predicting possible RNA and/or protein targets implicated in this multifactorial disease. We focused on 84 lncRNAs regulating the expression of pro-inflammatory and anti-inflammatory genes and miRNAs. In silico analysis was utilized to predict the interaction of lncRNAs with miRNAs, mRNAs, and proteins targets. Six lncRNAs were significantly down-regulated in OP patients compared to controls: <i<CEP83-AS1</i<, <i<RP11-84C13.1</i<, <i<CTC-487M23.5</i<, <i<GAS5</i<, <i<NCBP2-AS2</i<, and <i<SDCBP2-AS1</i<. Bioinformatic analyses identified HDCA2, PTX3, and FGF2 proteins as downstream targets of <i<CTC-487M23.5, GAS5</i<, and <i<RP11-84C13.1</i< lncRNAs mediated by the interaction with miRNAs implicated in OP pathogenesis, including <i<miR-21-5</i<p. Altogether, these data open a new regulatory mechanism of gene expression in bone homeostasis and could direct the development of future therapeutic approaches.
abstract_unstemmed	Osteoporosis (OP) is a multifactorial disease influenced by genetic, epigenetic, and environmental factors. One of the main causes of the bone homeostasis alteration is inflammation resulting in excessive bone resorption. Long non-coding RNAs (lncRNAs), have a crucial role in regulating many important biological processes in bone, including inflammation. We designed our study to identify lncRNAs misregulated in osteoblast primary cultures derived from OP patients (<i<n</i< = 4), and controls (CTRs, <i<n</i< = 4) with the aim of predicting possible RNA and/or protein targets implicated in this multifactorial disease. We focused on 84 lncRNAs regulating the expression of pro-inflammatory and anti-inflammatory genes and miRNAs. In silico analysis was utilized to predict the interaction of lncRNAs with miRNAs, mRNAs, and proteins targets. Six lncRNAs were significantly down-regulated in OP patients compared to controls: <i<CEP83-AS1</i<, <i<RP11-84C13.1</i<, <i<CTC-487M23.5</i<, <i<GAS5</i<, <i<NCBP2-AS2</i<, and <i<SDCBP2-AS1</i<. Bioinformatic analyses identified HDCA2, PTX3, and FGF2 proteins as downstream targets of <i<CTC-487M23.5, GAS5</i<, and <i<RP11-84C13.1</i< lncRNAs mediated by the interaction with miRNAs implicated in OP pathogenesis, including <i<miR-21-5</i<p. Altogether, these data open a new regulatory mechanism of gene expression in bone homeostasis and could direct the development of future therapeutic approaches.
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container_issue	3, p 65
title_short	Identification of Aberrantly-Expressed Long Non-Coding RNAs in Osteoblastic Cells from Osteoporotic Patients
url	https://doi.org/10.3390/biomedicines8030065 https://doaj.org/article/76e56cf2fc994f4bbb313ca45be30151 https://www.mdpi.com/2227-9059/8/3/65 https://doaj.org/toc/2227-9059
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author2	Massimo Santoro Mario Marini Virginia Veronica Visconti Anna Maria Rinaldi Monica Celi Giovanna D’Arcangelo Giuseppe Novelli Augusto Orlandi Virginia Tancredi Umberto Tarantino Annalisa Botta
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up_date	2024-07-03T23:46:27.003Z
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