A Membrane-Bound NAC-Like Transcription Factor OsNTL5 Represses the Flowering in Oryza sativa

In spite of short-day (SD) nature, rice (Oryza sativa) shares a conserved photoperiodic network for flowering control with long-day plants like Arabidopsis thaliana. Flowering or heading is an important agronomic trait in rice. NAC transcription factors (TFs) are well-conserved and one of the largest families of plant TFs. However, their function in flowering or heading time is not well-known yet. A preferential expression of a membrane-bound NAC-like TF OsNTL5 in developing leaves and panicles of rice indicated to us its putative role in flowering. To examine its function, three independent constructs was generated, one with a deletion in the C terminus membrane-spanning domain (OsNTL5∆C), OsNTL5∆C fused with the SRDX transcriptional repressor motif and OsNTL5∆C used with the VP16 activation domain under the Ubiquitin promoter to produce the overexpressing lines OsNTL5∆C, OsNTL5∆C-SRDX, and OsNTL5∆C-VP, respectively in rice. The OsNTL5∆C-VP line showed an early-flowering phenotype. In contrast to this, the plants with OsNTL5∆C and OsNTL5∆C-SRDX showed a very strong late-flowering phenotype, suggesting that OsNTL5 suppresses flowering as a transcriptional repressor. The protein subcellular localization assay suggested that N-terminal part of the OsNTL5 is localized to the nucleus after the protein is cleaved from its membrane-spanning domain at the C-terminal end and functions as a TF. Expression of flowering genes responsible for day length signals such as Early Heading Date 1 (Ehd1), Heading Date 3a (Hd3a), and Rice Flowering Locus T1 (RFT1) was significantly changed in the overexpression lines of OsNTL5∆C-VP, OsNTL5∆C, and OsNTL5∆C-SRDX as analyzed by Quantitative Real-time PCR. ChIP-qPCR and rice protoplasts assays indicate that OsNTL5 directly binds to the promoter of Ehd1 and negatively regulates the expression of Ehd1, which shows antagonistic photoperiodic expression patterns of OsNTL5 in a 24-h SD cycle. Hence in conclusion, the NAC-like TF OsNTL5 functions as a transcriptional repressor to suppress flowering in rice as an upstream factor of Ehd1. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Siyi Guo [verfasserIn] Shaojun Dai [verfasserIn] Prashant K. Singh [verfasserIn] Hongyan Wang [verfasserIn] Yanan Wang [verfasserIn] Jeanie L. H. Tan [verfasserIn] Wanyi Wee [verfasserIn] Toshiro Ito [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	flowering NAC transcription factor rice subcellular localization overexpression

Übergeordnetes Werk:	In: Frontiers in Plant Science - Frontiers Media S.A., 2011, 9(2018)
Übergeordnetes Werk:	volume:9 ; year:2018

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fpls.2018.00555

Katalog-ID:	DOAJ02706557X

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allfields	10.3389/fpls.2018.00555 doi (DE-627)DOAJ02706557X (DE-599)DOAJcbc6496eccef40b4948fbf694c952e6c DE-627 ger DE-627 rakwb eng SB1-1110 Siyi Guo verfasserin aut A Membrane-Bound NAC-Like Transcription Factor OsNTL5 Represses the Flowering in Oryza sativa 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In spite of short-day (SD) nature, rice (Oryza sativa) shares a conserved photoperiodic network for flowering control with long-day plants like Arabidopsis thaliana. Flowering or heading is an important agronomic trait in rice. NAC transcription factors (TFs) are well-conserved and one of the largest families of plant TFs. However, their function in flowering or heading time is not well-known yet. A preferential expression of a membrane-bound NAC-like TF OsNTL5 in developing leaves and panicles of rice indicated to us its putative role in flowering. To examine its function, three independent constructs was generated, one with a deletion in the C terminus membrane-spanning domain (OsNTL5∆C), OsNTL5∆C fused with the SRDX transcriptional repressor motif and OsNTL5∆C used with the VP16 activation domain under the Ubiquitin promoter to produce the overexpressing lines OsNTL5∆C, OsNTL5∆C-SRDX, and OsNTL5∆C-VP, respectively in rice. The OsNTL5∆C-VP line showed an early-flowering phenotype. In contrast to this, the plants with OsNTL5∆C and OsNTL5∆C-SRDX showed a very strong late-flowering phenotype, suggesting that OsNTL5 suppresses flowering as a transcriptional repressor. The protein subcellular localization assay suggested that N-terminal part of the OsNTL5 is localized to the nucleus after the protein is cleaved from its membrane-spanning domain at the C-terminal end and functions as a TF. Expression of flowering genes responsible for day length signals such as Early Heading Date 1 (Ehd1), Heading Date 3a (Hd3a), and Rice Flowering Locus T1 (RFT1) was significantly changed in the overexpression lines of OsNTL5∆C-VP, OsNTL5∆C, and OsNTL5∆C-SRDX as analyzed by Quantitative Real-time PCR. ChIP-qPCR and rice protoplasts assays indicate that OsNTL5 directly binds to the promoter of Ehd1 and negatively regulates the expression of Ehd1, which shows antagonistic photoperiodic expression patterns of OsNTL5 in a 24-h SD cycle. Hence in conclusion, the NAC-like TF OsNTL5 functions as a transcriptional repressor to suppress flowering in rice as an upstream factor of Ehd1. flowering NAC transcription factor rice subcellular localization overexpression Plant culture Siyi Guo verfasserin aut Shaojun Dai verfasserin aut Prashant K. Singh verfasserin aut Hongyan Wang verfasserin aut Yanan Wang verfasserin aut Jeanie L. H. Tan verfasserin aut Wanyi Wee verfasserin aut Toshiro Ito verfasserin aut Toshiro Ito verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 9(2018) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:9 year:2018 https://doi.org/10.3389/fpls.2018.00555 kostenfrei https://doaj.org/article/cbc6496eccef40b4948fbf694c952e6c kostenfrei http://journal.frontiersin.org/article/10.3389/fpls.2018.00555/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2018
spelling	10.3389/fpls.2018.00555 doi (DE-627)DOAJ02706557X (DE-599)DOAJcbc6496eccef40b4948fbf694c952e6c DE-627 ger DE-627 rakwb eng SB1-1110 Siyi Guo verfasserin aut A Membrane-Bound NAC-Like Transcription Factor OsNTL5 Represses the Flowering in Oryza sativa 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In spite of short-day (SD) nature, rice (Oryza sativa) shares a conserved photoperiodic network for flowering control with long-day plants like Arabidopsis thaliana. Flowering or heading is an important agronomic trait in rice. NAC transcription factors (TFs) are well-conserved and one of the largest families of plant TFs. However, their function in flowering or heading time is not well-known yet. A preferential expression of a membrane-bound NAC-like TF OsNTL5 in developing leaves and panicles of rice indicated to us its putative role in flowering. To examine its function, three independent constructs was generated, one with a deletion in the C terminus membrane-spanning domain (OsNTL5∆C), OsNTL5∆C fused with the SRDX transcriptional repressor motif and OsNTL5∆C used with the VP16 activation domain under the Ubiquitin promoter to produce the overexpressing lines OsNTL5∆C, OsNTL5∆C-SRDX, and OsNTL5∆C-VP, respectively in rice. The OsNTL5∆C-VP line showed an early-flowering phenotype. In contrast to this, the plants with OsNTL5∆C and OsNTL5∆C-SRDX showed a very strong late-flowering phenotype, suggesting that OsNTL5 suppresses flowering as a transcriptional repressor. The protein subcellular localization assay suggested that N-terminal part of the OsNTL5 is localized to the nucleus after the protein is cleaved from its membrane-spanning domain at the C-terminal end and functions as a TF. Expression of flowering genes responsible for day length signals such as Early Heading Date 1 (Ehd1), Heading Date 3a (Hd3a), and Rice Flowering Locus T1 (RFT1) was significantly changed in the overexpression lines of OsNTL5∆C-VP, OsNTL5∆C, and OsNTL5∆C-SRDX as analyzed by Quantitative Real-time PCR. ChIP-qPCR and rice protoplasts assays indicate that OsNTL5 directly binds to the promoter of Ehd1 and negatively regulates the expression of Ehd1, which shows antagonistic photoperiodic expression patterns of OsNTL5 in a 24-h SD cycle. Hence in conclusion, the NAC-like TF OsNTL5 functions as a transcriptional repressor to suppress flowering in rice as an upstream factor of Ehd1. flowering NAC transcription factor rice subcellular localization overexpression Plant culture Siyi Guo verfasserin aut Shaojun Dai verfasserin aut Prashant K. Singh verfasserin aut Hongyan Wang verfasserin aut Yanan Wang verfasserin aut Jeanie L. H. Tan verfasserin aut Wanyi Wee verfasserin aut Toshiro Ito verfasserin aut Toshiro Ito verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 9(2018) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:9 year:2018 https://doi.org/10.3389/fpls.2018.00555 kostenfrei https://doaj.org/article/cbc6496eccef40b4948fbf694c952e6c kostenfrei http://journal.frontiersin.org/article/10.3389/fpls.2018.00555/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2018
allfields_unstemmed	10.3389/fpls.2018.00555 doi (DE-627)DOAJ02706557X (DE-599)DOAJcbc6496eccef40b4948fbf694c952e6c DE-627 ger DE-627 rakwb eng SB1-1110 Siyi Guo verfasserin aut A Membrane-Bound NAC-Like Transcription Factor OsNTL5 Represses the Flowering in Oryza sativa 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In spite of short-day (SD) nature, rice (Oryza sativa) shares a conserved photoperiodic network for flowering control with long-day plants like Arabidopsis thaliana. Flowering or heading is an important agronomic trait in rice. NAC transcription factors (TFs) are well-conserved and one of the largest families of plant TFs. However, their function in flowering or heading time is not well-known yet. A preferential expression of a membrane-bound NAC-like TF OsNTL5 in developing leaves and panicles of rice indicated to us its putative role in flowering. To examine its function, three independent constructs was generated, one with a deletion in the C terminus membrane-spanning domain (OsNTL5∆C), OsNTL5∆C fused with the SRDX transcriptional repressor motif and OsNTL5∆C used with the VP16 activation domain under the Ubiquitin promoter to produce the overexpressing lines OsNTL5∆C, OsNTL5∆C-SRDX, and OsNTL5∆C-VP, respectively in rice. The OsNTL5∆C-VP line showed an early-flowering phenotype. In contrast to this, the plants with OsNTL5∆C and OsNTL5∆C-SRDX showed a very strong late-flowering phenotype, suggesting that OsNTL5 suppresses flowering as a transcriptional repressor. The protein subcellular localization assay suggested that N-terminal part of the OsNTL5 is localized to the nucleus after the protein is cleaved from its membrane-spanning domain at the C-terminal end and functions as a TF. Expression of flowering genes responsible for day length signals such as Early Heading Date 1 (Ehd1), Heading Date 3a (Hd3a), and Rice Flowering Locus T1 (RFT1) was significantly changed in the overexpression lines of OsNTL5∆C-VP, OsNTL5∆C, and OsNTL5∆C-SRDX as analyzed by Quantitative Real-time PCR. ChIP-qPCR and rice protoplasts assays indicate that OsNTL5 directly binds to the promoter of Ehd1 and negatively regulates the expression of Ehd1, which shows antagonistic photoperiodic expression patterns of OsNTL5 in a 24-h SD cycle. Hence in conclusion, the NAC-like TF OsNTL5 functions as a transcriptional repressor to suppress flowering in rice as an upstream factor of Ehd1. flowering NAC transcription factor rice subcellular localization overexpression Plant culture Siyi Guo verfasserin aut Shaojun Dai verfasserin aut Prashant K. Singh verfasserin aut Hongyan Wang verfasserin aut Yanan Wang verfasserin aut Jeanie L. H. Tan verfasserin aut Wanyi Wee verfasserin aut Toshiro Ito verfasserin aut Toshiro Ito verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 9(2018) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:9 year:2018 https://doi.org/10.3389/fpls.2018.00555 kostenfrei https://doaj.org/article/cbc6496eccef40b4948fbf694c952e6c kostenfrei http://journal.frontiersin.org/article/10.3389/fpls.2018.00555/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2018
allfieldsGer	10.3389/fpls.2018.00555 doi (DE-627)DOAJ02706557X (DE-599)DOAJcbc6496eccef40b4948fbf694c952e6c DE-627 ger DE-627 rakwb eng SB1-1110 Siyi Guo verfasserin aut A Membrane-Bound NAC-Like Transcription Factor OsNTL5 Represses the Flowering in Oryza sativa 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In spite of short-day (SD) nature, rice (Oryza sativa) shares a conserved photoperiodic network for flowering control with long-day plants like Arabidopsis thaliana. Flowering or heading is an important agronomic trait in rice. NAC transcription factors (TFs) are well-conserved and one of the largest families of plant TFs. However, their function in flowering or heading time is not well-known yet. A preferential expression of a membrane-bound NAC-like TF OsNTL5 in developing leaves and panicles of rice indicated to us its putative role in flowering. To examine its function, three independent constructs was generated, one with a deletion in the C terminus membrane-spanning domain (OsNTL5∆C), OsNTL5∆C fused with the SRDX transcriptional repressor motif and OsNTL5∆C used with the VP16 activation domain under the Ubiquitin promoter to produce the overexpressing lines OsNTL5∆C, OsNTL5∆C-SRDX, and OsNTL5∆C-VP, respectively in rice. The OsNTL5∆C-VP line showed an early-flowering phenotype. In contrast to this, the plants with OsNTL5∆C and OsNTL5∆C-SRDX showed a very strong late-flowering phenotype, suggesting that OsNTL5 suppresses flowering as a transcriptional repressor. The protein subcellular localization assay suggested that N-terminal part of the OsNTL5 is localized to the nucleus after the protein is cleaved from its membrane-spanning domain at the C-terminal end and functions as a TF. Expression of flowering genes responsible for day length signals such as Early Heading Date 1 (Ehd1), Heading Date 3a (Hd3a), and Rice Flowering Locus T1 (RFT1) was significantly changed in the overexpression lines of OsNTL5∆C-VP, OsNTL5∆C, and OsNTL5∆C-SRDX as analyzed by Quantitative Real-time PCR. ChIP-qPCR and rice protoplasts assays indicate that OsNTL5 directly binds to the promoter of Ehd1 and negatively regulates the expression of Ehd1, which shows antagonistic photoperiodic expression patterns of OsNTL5 in a 24-h SD cycle. Hence in conclusion, the NAC-like TF OsNTL5 functions as a transcriptional repressor to suppress flowering in rice as an upstream factor of Ehd1. flowering NAC transcription factor rice subcellular localization overexpression Plant culture Siyi Guo verfasserin aut Shaojun Dai verfasserin aut Prashant K. Singh verfasserin aut Hongyan Wang verfasserin aut Yanan Wang verfasserin aut Jeanie L. H. Tan verfasserin aut Wanyi Wee verfasserin aut Toshiro Ito verfasserin aut Toshiro Ito verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 9(2018) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:9 year:2018 https://doi.org/10.3389/fpls.2018.00555 kostenfrei https://doaj.org/article/cbc6496eccef40b4948fbf694c952e6c kostenfrei http://journal.frontiersin.org/article/10.3389/fpls.2018.00555/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2018
allfieldsSound	10.3389/fpls.2018.00555 doi (DE-627)DOAJ02706557X (DE-599)DOAJcbc6496eccef40b4948fbf694c952e6c DE-627 ger DE-627 rakwb eng SB1-1110 Siyi Guo verfasserin aut A Membrane-Bound NAC-Like Transcription Factor OsNTL5 Represses the Flowering in Oryza sativa 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In spite of short-day (SD) nature, rice (Oryza sativa) shares a conserved photoperiodic network for flowering control with long-day plants like Arabidopsis thaliana. Flowering or heading is an important agronomic trait in rice. NAC transcription factors (TFs) are well-conserved and one of the largest families of plant TFs. However, their function in flowering or heading time is not well-known yet. A preferential expression of a membrane-bound NAC-like TF OsNTL5 in developing leaves and panicles of rice indicated to us its putative role in flowering. To examine its function, three independent constructs was generated, one with a deletion in the C terminus membrane-spanning domain (OsNTL5∆C), OsNTL5∆C fused with the SRDX transcriptional repressor motif and OsNTL5∆C used with the VP16 activation domain under the Ubiquitin promoter to produce the overexpressing lines OsNTL5∆C, OsNTL5∆C-SRDX, and OsNTL5∆C-VP, respectively in rice. The OsNTL5∆C-VP line showed an early-flowering phenotype. In contrast to this, the plants with OsNTL5∆C and OsNTL5∆C-SRDX showed a very strong late-flowering phenotype, suggesting that OsNTL5 suppresses flowering as a transcriptional repressor. The protein subcellular localization assay suggested that N-terminal part of the OsNTL5 is localized to the nucleus after the protein is cleaved from its membrane-spanning domain at the C-terminal end and functions as a TF. Expression of flowering genes responsible for day length signals such as Early Heading Date 1 (Ehd1), Heading Date 3a (Hd3a), and Rice Flowering Locus T1 (RFT1) was significantly changed in the overexpression lines of OsNTL5∆C-VP, OsNTL5∆C, and OsNTL5∆C-SRDX as analyzed by Quantitative Real-time PCR. ChIP-qPCR and rice protoplasts assays indicate that OsNTL5 directly binds to the promoter of Ehd1 and negatively regulates the expression of Ehd1, which shows antagonistic photoperiodic expression patterns of OsNTL5 in a 24-h SD cycle. Hence in conclusion, the NAC-like TF OsNTL5 functions as a transcriptional repressor to suppress flowering in rice as an upstream factor of Ehd1. flowering NAC transcription factor rice subcellular localization overexpression Plant culture Siyi Guo verfasserin aut Shaojun Dai verfasserin aut Prashant K. Singh verfasserin aut Hongyan Wang verfasserin aut Yanan Wang verfasserin aut Jeanie L. H. Tan verfasserin aut Wanyi Wee verfasserin aut Toshiro Ito verfasserin aut Toshiro Ito verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 9(2018) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:9 year:2018 https://doi.org/10.3389/fpls.2018.00555 kostenfrei https://doaj.org/article/cbc6496eccef40b4948fbf694c952e6c kostenfrei http://journal.frontiersin.org/article/10.3389/fpls.2018.00555/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2018
language	English
source	In Frontiers in Plant Science 9(2018) volume:9 year:2018
sourceStr	In Frontiers in Plant Science 9(2018) volume:9 year:2018
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	flowering NAC transcription factor rice subcellular localization overexpression Plant culture
isfreeaccess_bool	true
container_title	Frontiers in Plant Science
authorswithroles_txt_mv	Siyi Guo @@aut@@ Shaojun Dai @@aut@@ Prashant K. Singh @@aut@@ Hongyan Wang @@aut@@ Yanan Wang @@aut@@ Jeanie L. H. Tan @@aut@@ Wanyi Wee @@aut@@ Toshiro Ito @@aut@@
publishDateDaySort_date	2018-01-01T00:00:00Z
hierarchy_top_id	662359240
id	DOAJ02706557X
language_de	englisch
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callnumber-first	S - Agriculture
author	Siyi Guo
spellingShingle	Siyi Guo misc SB1-1110 misc flowering misc NAC transcription factor misc rice misc subcellular localization misc overexpression misc Plant culture A Membrane-Bound NAC-Like Transcription Factor OsNTL5 Represses the Flowering in Oryza sativa
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topic_title	SB1-1110 A Membrane-Bound NAC-Like Transcription Factor OsNTL5 Represses the Flowering in Oryza sativa flowering NAC transcription factor rice subcellular localization overexpression
topic	misc SB1-1110 misc flowering misc NAC transcription factor misc rice misc subcellular localization misc overexpression misc Plant culture
topic_unstemmed	misc SB1-1110 misc flowering misc NAC transcription factor misc rice misc subcellular localization misc overexpression misc Plant culture
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title	A Membrane-Bound NAC-Like Transcription Factor OsNTL5 Represses the Flowering in Oryza sativa
ctrlnum	(DE-627)DOAJ02706557X (DE-599)DOAJcbc6496eccef40b4948fbf694c952e6c
title_full	A Membrane-Bound NAC-Like Transcription Factor OsNTL5 Represses the Flowering in Oryza sativa
author_sort	Siyi Guo
journal	Frontiers in Plant Science
journalStr	Frontiers in Plant Science
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author_browse	Siyi Guo Shaojun Dai Prashant K. Singh Hongyan Wang Yanan Wang Jeanie L. H. Tan Wanyi Wee Toshiro Ito
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author-letter	Siyi Guo
doi_str_mv	10.3389/fpls.2018.00555
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title_sort	membrane-bound nac-like transcription factor osntl5 represses the flowering in oryza sativa
callnumber	SB1-1110
title_auth	A Membrane-Bound NAC-Like Transcription Factor OsNTL5 Represses the Flowering in Oryza sativa
abstract	In spite of short-day (SD) nature, rice (Oryza sativa) shares a conserved photoperiodic network for flowering control with long-day plants like Arabidopsis thaliana. Flowering or heading is an important agronomic trait in rice. NAC transcription factors (TFs) are well-conserved and one of the largest families of plant TFs. However, their function in flowering or heading time is not well-known yet. A preferential expression of a membrane-bound NAC-like TF OsNTL5 in developing leaves and panicles of rice indicated to us its putative role in flowering. To examine its function, three independent constructs was generated, one with a deletion in the C terminus membrane-spanning domain (OsNTL5∆C), OsNTL5∆C fused with the SRDX transcriptional repressor motif and OsNTL5∆C used with the VP16 activation domain under the Ubiquitin promoter to produce the overexpressing lines OsNTL5∆C, OsNTL5∆C-SRDX, and OsNTL5∆C-VP, respectively in rice. The OsNTL5∆C-VP line showed an early-flowering phenotype. In contrast to this, the plants with OsNTL5∆C and OsNTL5∆C-SRDX showed a very strong late-flowering phenotype, suggesting that OsNTL5 suppresses flowering as a transcriptional repressor. The protein subcellular localization assay suggested that N-terminal part of the OsNTL5 is localized to the nucleus after the protein is cleaved from its membrane-spanning domain at the C-terminal end and functions as a TF. Expression of flowering genes responsible for day length signals such as Early Heading Date 1 (Ehd1), Heading Date 3a (Hd3a), and Rice Flowering Locus T1 (RFT1) was significantly changed in the overexpression lines of OsNTL5∆C-VP, OsNTL5∆C, and OsNTL5∆C-SRDX as analyzed by Quantitative Real-time PCR. ChIP-qPCR and rice protoplasts assays indicate that OsNTL5 directly binds to the promoter of Ehd1 and negatively regulates the expression of Ehd1, which shows antagonistic photoperiodic expression patterns of OsNTL5 in a 24-h SD cycle. Hence in conclusion, the NAC-like TF OsNTL5 functions as a transcriptional repressor to suppress flowering in rice as an upstream factor of Ehd1.
abstractGer	In spite of short-day (SD) nature, rice (Oryza sativa) shares a conserved photoperiodic network for flowering control with long-day plants like Arabidopsis thaliana. Flowering or heading is an important agronomic trait in rice. NAC transcription factors (TFs) are well-conserved and one of the largest families of plant TFs. However, their function in flowering or heading time is not well-known yet. A preferential expression of a membrane-bound NAC-like TF OsNTL5 in developing leaves and panicles of rice indicated to us its putative role in flowering. To examine its function, three independent constructs was generated, one with a deletion in the C terminus membrane-spanning domain (OsNTL5∆C), OsNTL5∆C fused with the SRDX transcriptional repressor motif and OsNTL5∆C used with the VP16 activation domain under the Ubiquitin promoter to produce the overexpressing lines OsNTL5∆C, OsNTL5∆C-SRDX, and OsNTL5∆C-VP, respectively in rice. The OsNTL5∆C-VP line showed an early-flowering phenotype. In contrast to this, the plants with OsNTL5∆C and OsNTL5∆C-SRDX showed a very strong late-flowering phenotype, suggesting that OsNTL5 suppresses flowering as a transcriptional repressor. The protein subcellular localization assay suggested that N-terminal part of the OsNTL5 is localized to the nucleus after the protein is cleaved from its membrane-spanning domain at the C-terminal end and functions as a TF. Expression of flowering genes responsible for day length signals such as Early Heading Date 1 (Ehd1), Heading Date 3a (Hd3a), and Rice Flowering Locus T1 (RFT1) was significantly changed in the overexpression lines of OsNTL5∆C-VP, OsNTL5∆C, and OsNTL5∆C-SRDX as analyzed by Quantitative Real-time PCR. ChIP-qPCR and rice protoplasts assays indicate that OsNTL5 directly binds to the promoter of Ehd1 and negatively regulates the expression of Ehd1, which shows antagonistic photoperiodic expression patterns of OsNTL5 in a 24-h SD cycle. Hence in conclusion, the NAC-like TF OsNTL5 functions as a transcriptional repressor to suppress flowering in rice as an upstream factor of Ehd1.
abstract_unstemmed	In spite of short-day (SD) nature, rice (Oryza sativa) shares a conserved photoperiodic network for flowering control with long-day plants like Arabidopsis thaliana. Flowering or heading is an important agronomic trait in rice. NAC transcription factors (TFs) are well-conserved and one of the largest families of plant TFs. However, their function in flowering or heading time is not well-known yet. A preferential expression of a membrane-bound NAC-like TF OsNTL5 in developing leaves and panicles of rice indicated to us its putative role in flowering. To examine its function, three independent constructs was generated, one with a deletion in the C terminus membrane-spanning domain (OsNTL5∆C), OsNTL5∆C fused with the SRDX transcriptional repressor motif and OsNTL5∆C used with the VP16 activation domain under the Ubiquitin promoter to produce the overexpressing lines OsNTL5∆C, OsNTL5∆C-SRDX, and OsNTL5∆C-VP, respectively in rice. The OsNTL5∆C-VP line showed an early-flowering phenotype. In contrast to this, the plants with OsNTL5∆C and OsNTL5∆C-SRDX showed a very strong late-flowering phenotype, suggesting that OsNTL5 suppresses flowering as a transcriptional repressor. The protein subcellular localization assay suggested that N-terminal part of the OsNTL5 is localized to the nucleus after the protein is cleaved from its membrane-spanning domain at the C-terminal end and functions as a TF. Expression of flowering genes responsible for day length signals such as Early Heading Date 1 (Ehd1), Heading Date 3a (Hd3a), and Rice Flowering Locus T1 (RFT1) was significantly changed in the overexpression lines of OsNTL5∆C-VP, OsNTL5∆C, and OsNTL5∆C-SRDX as analyzed by Quantitative Real-time PCR. ChIP-qPCR and rice protoplasts assays indicate that OsNTL5 directly binds to the promoter of Ehd1 and negatively regulates the expression of Ehd1, which shows antagonistic photoperiodic expression patterns of OsNTL5 in a 24-h SD cycle. Hence in conclusion, the NAC-like TF OsNTL5 functions as a transcriptional repressor to suppress flowering in rice as an upstream factor of Ehd1.
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title_short	A Membrane-Bound NAC-Like Transcription Factor OsNTL5 Represses the Flowering in Oryza sativa
url	https://doi.org/10.3389/fpls.2018.00555 https://doaj.org/article/cbc6496eccef40b4948fbf694c952e6c http://journal.frontiersin.org/article/10.3389/fpls.2018.00555/full https://doaj.org/toc/1664-462X
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A Membrane-Bound NAC-Like Transcription Factor OsNTL5 Represses the Flowering in Oryza sativa

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