Possible Toxic Mechanisms of Deoxynivalenol (DON) Exposure to Intestinal Barrier Damage and Dysbiosis of the Gut Microbiota in Laying Hens

Deoxynivalenol is one the of most common mycotoxins in cereals and grains and causes a serious health threat to poultry and farm animals. Our previous study found that DON decreased the production performance of laying hens. It has been reported that DON could exert significant toxic effects on the intestinal barrier and microbiota. However, whether the decline of laying performance is related to intestinal barrier damage, and the underlying mechanisms of DON induced intestine function injury remain largely unclear in laying hens. In this study, 80 Hy-line brown laying hens at 26 weeks were randomly divided into 0, 1, 5 and 10 mg/kg.bw (body weight) DON daily for 6 weeks. The morphology of the duodenum, the expression of inflammation factors and tight junction proteins, and the diversity and abundance of microbiota were analyzed in different levels of DON treated to laying hens. The results demonstrated that the mucosal detachment and reduction of the villi number were presented in different DON treated groups with a dose-effect manner. Additionally, the genes expression of pro-inflammatory factors IL-1β, IL-8, TNF-α and anti-inflammatory factors IL-10 were increased or decreased at 5 and 10 mg/kg.bw DON groups, respectively. The levels of ZO-1 and claudin-1 expression were significantly decreased in 5 and 10 mg/kg.bw DON groups. Moreover, the alpha diversity including Chao, ACE and Shannon indices were all reduced in DON treated groups. At the phylum level, <i<Firmicutes</i< and <i<Actinobacteria</i< and <i<Bacteroidetes</i<, <i<Proteobacteria</i<, and <i<Spirochaetes</i< were decreased and increased in 10 mg/kg.bw DON group, respectively. At the genus levels, the relative abundance of <i<Clostridium</i< and <i<Lactobacillus</i< in 5 and 10 mg/kg.bw DON groups, and <i<Alkanindiges</i< and <i<Spirochaeta</i< in the 10 mg/kg.bw DON were significantly decreased and increased, respectively. Moreover, there were significant correlation between the expression of tight junction proteins and the relative abundance of <i<Lactobacillus</i< and <i<Succinispira.</i< These results indicated that DON exposure to the laying hens can induce the inflammation and disrupt intestinal tight junctions, suggesting that DON can directly damage barrier function, which may be closely related to the dysbiosis of intestinal microbiota. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Xiaohu Zhai [verfasserIn] Zhi Qiu [verfasserIn] Lihua Wang [verfasserIn] Youwen Luo [verfasserIn] Weihua He [verfasserIn] Junhua Yang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	deoxynivalenol laying hens intestinal inflammation barrier function intestinal microbiota

Übergeordnetes Werk:	In: Toxins - MDPI AG, 2010, 14(2022), 10, p 682
Übergeordnetes Werk:	volume:14 ; year:2022 ; number:10, p 682

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/toxins14100682

Katalog-ID:	DOAJ027402576

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allfields	10.3390/toxins14100682 doi (DE-627)DOAJ027402576 (DE-599)DOAJ74a3a0a5423943e9b256030b25ab08ab DE-627 ger DE-627 rakwb eng Xiaohu Zhai verfasserin aut Possible Toxic Mechanisms of Deoxynivalenol (DON) Exposure to Intestinal Barrier Damage and Dysbiosis of the Gut Microbiota in Laying Hens 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Deoxynivalenol is one the of most common mycotoxins in cereals and grains and causes a serious health threat to poultry and farm animals. Our previous study found that DON decreased the production performance of laying hens. It has been reported that DON could exert significant toxic effects on the intestinal barrier and microbiota. However, whether the decline of laying performance is related to intestinal barrier damage, and the underlying mechanisms of DON induced intestine function injury remain largely unclear in laying hens. In this study, 80 Hy-line brown laying hens at 26 weeks were randomly divided into 0, 1, 5 and 10 mg/kg.bw (body weight) DON daily for 6 weeks. The morphology of the duodenum, the expression of inflammation factors and tight junction proteins, and the diversity and abundance of microbiota were analyzed in different levels of DON treated to laying hens. The results demonstrated that the mucosal detachment and reduction of the villi number were presented in different DON treated groups with a dose-effect manner. Additionally, the genes expression of pro-inflammatory factors IL-1β, IL-8, TNF-α and anti-inflammatory factors IL-10 were increased or decreased at 5 and 10 mg/kg.bw DON groups, respectively. The levels of ZO-1 and claudin-1 expression were significantly decreased in 5 and 10 mg/kg.bw DON groups. Moreover, the alpha diversity including Chao, ACE and Shannon indices were all reduced in DON treated groups. At the phylum level, <i<Firmicutes</i< and <i<Actinobacteria</i< and <i<Bacteroidetes</i<, <i<Proteobacteria</i<, and <i<Spirochaetes</i< were decreased and increased in 10 mg/kg.bw DON group, respectively. At the genus levels, the relative abundance of <i<Clostridium</i< and <i<Lactobacillus</i< in 5 and 10 mg/kg.bw DON groups, and <i<Alkanindiges</i< and <i<Spirochaeta</i< in the 10 mg/kg.bw DON were significantly decreased and increased, respectively. Moreover, there were significant correlation between the expression of tight junction proteins and the relative abundance of <i<Lactobacillus</i< and <i<Succinispira.</i< These results indicated that DON exposure to the laying hens can induce the inflammation and disrupt intestinal tight junctions, suggesting that DON can directly damage barrier function, which may be closely related to the dysbiosis of intestinal microbiota. deoxynivalenol laying hens intestinal inflammation barrier function intestinal microbiota Medicine R Zhi Qiu verfasserin aut Lihua Wang verfasserin aut Youwen Luo verfasserin aut Weihua He verfasserin aut Junhua Yang verfasserin aut In Toxins MDPI AG, 2010 14(2022), 10, p 682 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:14 year:2022 number:10, p 682 https://doi.org/10.3390/toxins14100682 kostenfrei https://doaj.org/article/74a3a0a5423943e9b256030b25ab08ab kostenfrei https://www.mdpi.com/2072-6651/14/10/682 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 10, p 682
spelling	10.3390/toxins14100682 doi (DE-627)DOAJ027402576 (DE-599)DOAJ74a3a0a5423943e9b256030b25ab08ab DE-627 ger DE-627 rakwb eng Xiaohu Zhai verfasserin aut Possible Toxic Mechanisms of Deoxynivalenol (DON) Exposure to Intestinal Barrier Damage and Dysbiosis of the Gut Microbiota in Laying Hens 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Deoxynivalenol is one the of most common mycotoxins in cereals and grains and causes a serious health threat to poultry and farm animals. Our previous study found that DON decreased the production performance of laying hens. It has been reported that DON could exert significant toxic effects on the intestinal barrier and microbiota. However, whether the decline of laying performance is related to intestinal barrier damage, and the underlying mechanisms of DON induced intestine function injury remain largely unclear in laying hens. In this study, 80 Hy-line brown laying hens at 26 weeks were randomly divided into 0, 1, 5 and 10 mg/kg.bw (body weight) DON daily for 6 weeks. The morphology of the duodenum, the expression of inflammation factors and tight junction proteins, and the diversity and abundance of microbiota were analyzed in different levels of DON treated to laying hens. The results demonstrated that the mucosal detachment and reduction of the villi number were presented in different DON treated groups with a dose-effect manner. Additionally, the genes expression of pro-inflammatory factors IL-1β, IL-8, TNF-α and anti-inflammatory factors IL-10 were increased or decreased at 5 and 10 mg/kg.bw DON groups, respectively. The levels of ZO-1 and claudin-1 expression were significantly decreased in 5 and 10 mg/kg.bw DON groups. Moreover, the alpha diversity including Chao, ACE and Shannon indices were all reduced in DON treated groups. At the phylum level, <i<Firmicutes</i< and <i<Actinobacteria</i< and <i<Bacteroidetes</i<, <i<Proteobacteria</i<, and <i<Spirochaetes</i< were decreased and increased in 10 mg/kg.bw DON group, respectively. At the genus levels, the relative abundance of <i<Clostridium</i< and <i<Lactobacillus</i< in 5 and 10 mg/kg.bw DON groups, and <i<Alkanindiges</i< and <i<Spirochaeta</i< in the 10 mg/kg.bw DON were significantly decreased and increased, respectively. Moreover, there were significant correlation between the expression of tight junction proteins and the relative abundance of <i<Lactobacillus</i< and <i<Succinispira.</i< These results indicated that DON exposure to the laying hens can induce the inflammation and disrupt intestinal tight junctions, suggesting that DON can directly damage barrier function, which may be closely related to the dysbiosis of intestinal microbiota. deoxynivalenol laying hens intestinal inflammation barrier function intestinal microbiota Medicine R Zhi Qiu verfasserin aut Lihua Wang verfasserin aut Youwen Luo verfasserin aut Weihua He verfasserin aut Junhua Yang verfasserin aut In Toxins MDPI AG, 2010 14(2022), 10, p 682 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:14 year:2022 number:10, p 682 https://doi.org/10.3390/toxins14100682 kostenfrei https://doaj.org/article/74a3a0a5423943e9b256030b25ab08ab kostenfrei https://www.mdpi.com/2072-6651/14/10/682 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 10, p 682
allfields_unstemmed	10.3390/toxins14100682 doi (DE-627)DOAJ027402576 (DE-599)DOAJ74a3a0a5423943e9b256030b25ab08ab DE-627 ger DE-627 rakwb eng Xiaohu Zhai verfasserin aut Possible Toxic Mechanisms of Deoxynivalenol (DON) Exposure to Intestinal Barrier Damage and Dysbiosis of the Gut Microbiota in Laying Hens 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Deoxynivalenol is one the of most common mycotoxins in cereals and grains and causes a serious health threat to poultry and farm animals. Our previous study found that DON decreased the production performance of laying hens. It has been reported that DON could exert significant toxic effects on the intestinal barrier and microbiota. However, whether the decline of laying performance is related to intestinal barrier damage, and the underlying mechanisms of DON induced intestine function injury remain largely unclear in laying hens. In this study, 80 Hy-line brown laying hens at 26 weeks were randomly divided into 0, 1, 5 and 10 mg/kg.bw (body weight) DON daily for 6 weeks. The morphology of the duodenum, the expression of inflammation factors and tight junction proteins, and the diversity and abundance of microbiota were analyzed in different levels of DON treated to laying hens. The results demonstrated that the mucosal detachment and reduction of the villi number were presented in different DON treated groups with a dose-effect manner. Additionally, the genes expression of pro-inflammatory factors IL-1β, IL-8, TNF-α and anti-inflammatory factors IL-10 were increased or decreased at 5 and 10 mg/kg.bw DON groups, respectively. The levels of ZO-1 and claudin-1 expression were significantly decreased in 5 and 10 mg/kg.bw DON groups. Moreover, the alpha diversity including Chao, ACE and Shannon indices were all reduced in DON treated groups. At the phylum level, <i<Firmicutes</i< and <i<Actinobacteria</i< and <i<Bacteroidetes</i<, <i<Proteobacteria</i<, and <i<Spirochaetes</i< were decreased and increased in 10 mg/kg.bw DON group, respectively. At the genus levels, the relative abundance of <i<Clostridium</i< and <i<Lactobacillus</i< in 5 and 10 mg/kg.bw DON groups, and <i<Alkanindiges</i< and <i<Spirochaeta</i< in the 10 mg/kg.bw DON were significantly decreased and increased, respectively. Moreover, there were significant correlation between the expression of tight junction proteins and the relative abundance of <i<Lactobacillus</i< and <i<Succinispira.</i< These results indicated that DON exposure to the laying hens can induce the inflammation and disrupt intestinal tight junctions, suggesting that DON can directly damage barrier function, which may be closely related to the dysbiosis of intestinal microbiota. deoxynivalenol laying hens intestinal inflammation barrier function intestinal microbiota Medicine R Zhi Qiu verfasserin aut Lihua Wang verfasserin aut Youwen Luo verfasserin aut Weihua He verfasserin aut Junhua Yang verfasserin aut In Toxins MDPI AG, 2010 14(2022), 10, p 682 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:14 year:2022 number:10, p 682 https://doi.org/10.3390/toxins14100682 kostenfrei https://doaj.org/article/74a3a0a5423943e9b256030b25ab08ab kostenfrei https://www.mdpi.com/2072-6651/14/10/682 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 10, p 682
allfieldsGer	10.3390/toxins14100682 doi (DE-627)DOAJ027402576 (DE-599)DOAJ74a3a0a5423943e9b256030b25ab08ab DE-627 ger DE-627 rakwb eng Xiaohu Zhai verfasserin aut Possible Toxic Mechanisms of Deoxynivalenol (DON) Exposure to Intestinal Barrier Damage and Dysbiosis of the Gut Microbiota in Laying Hens 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Deoxynivalenol is one the of most common mycotoxins in cereals and grains and causes a serious health threat to poultry and farm animals. Our previous study found that DON decreased the production performance of laying hens. It has been reported that DON could exert significant toxic effects on the intestinal barrier and microbiota. However, whether the decline of laying performance is related to intestinal barrier damage, and the underlying mechanisms of DON induced intestine function injury remain largely unclear in laying hens. In this study, 80 Hy-line brown laying hens at 26 weeks were randomly divided into 0, 1, 5 and 10 mg/kg.bw (body weight) DON daily for 6 weeks. The morphology of the duodenum, the expression of inflammation factors and tight junction proteins, and the diversity and abundance of microbiota were analyzed in different levels of DON treated to laying hens. The results demonstrated that the mucosal detachment and reduction of the villi number were presented in different DON treated groups with a dose-effect manner. Additionally, the genes expression of pro-inflammatory factors IL-1β, IL-8, TNF-α and anti-inflammatory factors IL-10 were increased or decreased at 5 and 10 mg/kg.bw DON groups, respectively. The levels of ZO-1 and claudin-1 expression were significantly decreased in 5 and 10 mg/kg.bw DON groups. Moreover, the alpha diversity including Chao, ACE and Shannon indices were all reduced in DON treated groups. At the phylum level, <i<Firmicutes</i< and <i<Actinobacteria</i< and <i<Bacteroidetes</i<, <i<Proteobacteria</i<, and <i<Spirochaetes</i< were decreased and increased in 10 mg/kg.bw DON group, respectively. At the genus levels, the relative abundance of <i<Clostridium</i< and <i<Lactobacillus</i< in 5 and 10 mg/kg.bw DON groups, and <i<Alkanindiges</i< and <i<Spirochaeta</i< in the 10 mg/kg.bw DON were significantly decreased and increased, respectively. Moreover, there were significant correlation between the expression of tight junction proteins and the relative abundance of <i<Lactobacillus</i< and <i<Succinispira.</i< These results indicated that DON exposure to the laying hens can induce the inflammation and disrupt intestinal tight junctions, suggesting that DON can directly damage barrier function, which may be closely related to the dysbiosis of intestinal microbiota. deoxynivalenol laying hens intestinal inflammation barrier function intestinal microbiota Medicine R Zhi Qiu verfasserin aut Lihua Wang verfasserin aut Youwen Luo verfasserin aut Weihua He verfasserin aut Junhua Yang verfasserin aut In Toxins MDPI AG, 2010 14(2022), 10, p 682 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:14 year:2022 number:10, p 682 https://doi.org/10.3390/toxins14100682 kostenfrei https://doaj.org/article/74a3a0a5423943e9b256030b25ab08ab kostenfrei https://www.mdpi.com/2072-6651/14/10/682 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 10, p 682
allfieldsSound	10.3390/toxins14100682 doi (DE-627)DOAJ027402576 (DE-599)DOAJ74a3a0a5423943e9b256030b25ab08ab DE-627 ger DE-627 rakwb eng Xiaohu Zhai verfasserin aut Possible Toxic Mechanisms of Deoxynivalenol (DON) Exposure to Intestinal Barrier Damage and Dysbiosis of the Gut Microbiota in Laying Hens 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Deoxynivalenol is one the of most common mycotoxins in cereals and grains and causes a serious health threat to poultry and farm animals. Our previous study found that DON decreased the production performance of laying hens. It has been reported that DON could exert significant toxic effects on the intestinal barrier and microbiota. However, whether the decline of laying performance is related to intestinal barrier damage, and the underlying mechanisms of DON induced intestine function injury remain largely unclear in laying hens. In this study, 80 Hy-line brown laying hens at 26 weeks were randomly divided into 0, 1, 5 and 10 mg/kg.bw (body weight) DON daily for 6 weeks. The morphology of the duodenum, the expression of inflammation factors and tight junction proteins, and the diversity and abundance of microbiota were analyzed in different levels of DON treated to laying hens. The results demonstrated that the mucosal detachment and reduction of the villi number were presented in different DON treated groups with a dose-effect manner. Additionally, the genes expression of pro-inflammatory factors IL-1β, IL-8, TNF-α and anti-inflammatory factors IL-10 were increased or decreased at 5 and 10 mg/kg.bw DON groups, respectively. The levels of ZO-1 and claudin-1 expression were significantly decreased in 5 and 10 mg/kg.bw DON groups. Moreover, the alpha diversity including Chao, ACE and Shannon indices were all reduced in DON treated groups. At the phylum level, <i<Firmicutes</i< and <i<Actinobacteria</i< and <i<Bacteroidetes</i<, <i<Proteobacteria</i<, and <i<Spirochaetes</i< were decreased and increased in 10 mg/kg.bw DON group, respectively. At the genus levels, the relative abundance of <i<Clostridium</i< and <i<Lactobacillus</i< in 5 and 10 mg/kg.bw DON groups, and <i<Alkanindiges</i< and <i<Spirochaeta</i< in the 10 mg/kg.bw DON were significantly decreased and increased, respectively. Moreover, there were significant correlation between the expression of tight junction proteins and the relative abundance of <i<Lactobacillus</i< and <i<Succinispira.</i< These results indicated that DON exposure to the laying hens can induce the inflammation and disrupt intestinal tight junctions, suggesting that DON can directly damage barrier function, which may be closely related to the dysbiosis of intestinal microbiota. deoxynivalenol laying hens intestinal inflammation barrier function intestinal microbiota Medicine R Zhi Qiu verfasserin aut Lihua Wang verfasserin aut Youwen Luo verfasserin aut Weihua He verfasserin aut Junhua Yang verfasserin aut In Toxins MDPI AG, 2010 14(2022), 10, p 682 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:14 year:2022 number:10, p 682 https://doi.org/10.3390/toxins14100682 kostenfrei https://doaj.org/article/74a3a0a5423943e9b256030b25ab08ab kostenfrei https://www.mdpi.com/2072-6651/14/10/682 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2022 10, p 682
language	English
source	In Toxins 14(2022), 10, p 682 volume:14 year:2022 number:10, p 682
sourceStr	In Toxins 14(2022), 10, p 682 volume:14 year:2022 number:10, p 682
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	deoxynivalenol laying hens intestinal inflammation barrier function intestinal microbiota Medicine R
isfreeaccess_bool	true
container_title	Toxins
authorswithroles_txt_mv	Xiaohu Zhai @@aut@@ Zhi Qiu @@aut@@ Lihua Wang @@aut@@ Youwen Luo @@aut@@ Weihua He @@aut@@ Junhua Yang @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	610604236
id	DOAJ027402576
language_de	englisch
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Our previous study found that DON decreased the production performance of laying hens. It has been reported that DON could exert significant toxic effects on the intestinal barrier and microbiota. However, whether the decline of laying performance is related to intestinal barrier damage, and the underlying mechanisms of DON induced intestine function injury remain largely unclear in laying hens. In this study, 80 Hy-line brown laying hens at 26 weeks were randomly divided into 0, 1, 5 and 10 mg/kg.bw (body weight) DON daily for 6 weeks. The morphology of the duodenum, the expression of inflammation factors and tight junction proteins, and the diversity and abundance of microbiota were analyzed in different levels of DON treated to laying hens. The results demonstrated that the mucosal detachment and reduction of the villi number were presented in different DON treated groups with a dose-effect manner. Additionally, the genes expression of pro-inflammatory factors IL-1β, IL-8, TNF-α and anti-inflammatory factors IL-10 were increased or decreased at 5 and 10 mg/kg.bw DON groups, respectively. The levels of ZO-1 and claudin-1 expression were significantly decreased in 5 and 10 mg/kg.bw DON groups. Moreover, the alpha diversity including Chao, ACE and Shannon indices were all reduced in DON treated groups. At the phylum level, <i<Firmicutes</i< and <i<Actinobacteria</i< and <i<Bacteroidetes</i<, <i<Proteobacteria</i<, and <i<Spirochaetes</i< were decreased and increased in 10 mg/kg.bw DON group, respectively. At the genus levels, the relative abundance of <i<Clostridium</i< and <i<Lactobacillus</i< in 5 and 10 mg/kg.bw DON groups, and <i<Alkanindiges</i< and <i<Spirochaeta</i< in the 10 mg/kg.bw DON were significantly decreased and increased, respectively. 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author	Xiaohu Zhai
spellingShingle	Xiaohu Zhai misc deoxynivalenol misc laying hens misc intestinal inflammation misc barrier function misc intestinal microbiota misc Medicine misc R Possible Toxic Mechanisms of Deoxynivalenol (DON) Exposure to Intestinal Barrier Damage and Dysbiosis of the Gut Microbiota in Laying Hens
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topic_title	Possible Toxic Mechanisms of Deoxynivalenol (DON) Exposure to Intestinal Barrier Damage and Dysbiosis of the Gut Microbiota in Laying Hens deoxynivalenol laying hens intestinal inflammation barrier function intestinal microbiota
topic	misc deoxynivalenol misc laying hens misc intestinal inflammation misc barrier function misc intestinal microbiota misc Medicine misc R
topic_unstemmed	misc deoxynivalenol misc laying hens misc intestinal inflammation misc barrier function misc intestinal microbiota misc Medicine misc R
topic_browse	misc deoxynivalenol misc laying hens misc intestinal inflammation misc barrier function misc intestinal microbiota misc Medicine misc R
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title	Possible Toxic Mechanisms of Deoxynivalenol (DON) Exposure to Intestinal Barrier Damage and Dysbiosis of the Gut Microbiota in Laying Hens
ctrlnum	(DE-627)DOAJ027402576 (DE-599)DOAJ74a3a0a5423943e9b256030b25ab08ab
title_full	Possible Toxic Mechanisms of Deoxynivalenol (DON) Exposure to Intestinal Barrier Damage and Dysbiosis of the Gut Microbiota in Laying Hens
author_sort	Xiaohu Zhai
journal	Toxins
journalStr	Toxins
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author_browse	Xiaohu Zhai Zhi Qiu Lihua Wang Youwen Luo Weihua He Junhua Yang
container_volume	14
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author-letter	Xiaohu Zhai
doi_str_mv	10.3390/toxins14100682
author2-role	verfasserin
title_sort	possible toxic mechanisms of deoxynivalenol (don) exposure to intestinal barrier damage and dysbiosis of the gut microbiota in laying hens
title_auth	Possible Toxic Mechanisms of Deoxynivalenol (DON) Exposure to Intestinal Barrier Damage and Dysbiosis of the Gut Microbiota in Laying Hens
abstract	Deoxynivalenol is one the of most common mycotoxins in cereals and grains and causes a serious health threat to poultry and farm animals. Our previous study found that DON decreased the production performance of laying hens. It has been reported that DON could exert significant toxic effects on the intestinal barrier and microbiota. However, whether the decline of laying performance is related to intestinal barrier damage, and the underlying mechanisms of DON induced intestine function injury remain largely unclear in laying hens. In this study, 80 Hy-line brown laying hens at 26 weeks were randomly divided into 0, 1, 5 and 10 mg/kg.bw (body weight) DON daily for 6 weeks. The morphology of the duodenum, the expression of inflammation factors and tight junction proteins, and the diversity and abundance of microbiota were analyzed in different levels of DON treated to laying hens. The results demonstrated that the mucosal detachment and reduction of the villi number were presented in different DON treated groups with a dose-effect manner. Additionally, the genes expression of pro-inflammatory factors IL-1β, IL-8, TNF-α and anti-inflammatory factors IL-10 were increased or decreased at 5 and 10 mg/kg.bw DON groups, respectively. The levels of ZO-1 and claudin-1 expression were significantly decreased in 5 and 10 mg/kg.bw DON groups. Moreover, the alpha diversity including Chao, ACE and Shannon indices were all reduced in DON treated groups. At the phylum level, <i<Firmicutes</i< and <i<Actinobacteria</i< and <i<Bacteroidetes</i<, <i<Proteobacteria</i<, and <i<Spirochaetes</i< were decreased and increased in 10 mg/kg.bw DON group, respectively. At the genus levels, the relative abundance of <i<Clostridium</i< and <i<Lactobacillus</i< in 5 and 10 mg/kg.bw DON groups, and <i<Alkanindiges</i< and <i<Spirochaeta</i< in the 10 mg/kg.bw DON were significantly decreased and increased, respectively. Moreover, there were significant correlation between the expression of tight junction proteins and the relative abundance of <i<Lactobacillus</i< and <i<Succinispira.</i< These results indicated that DON exposure to the laying hens can induce the inflammation and disrupt intestinal tight junctions, suggesting that DON can directly damage barrier function, which may be closely related to the dysbiosis of intestinal microbiota.
abstractGer	Deoxynivalenol is one the of most common mycotoxins in cereals and grains and causes a serious health threat to poultry and farm animals. Our previous study found that DON decreased the production performance of laying hens. It has been reported that DON could exert significant toxic effects on the intestinal barrier and microbiota. However, whether the decline of laying performance is related to intestinal barrier damage, and the underlying mechanisms of DON induced intestine function injury remain largely unclear in laying hens. In this study, 80 Hy-line brown laying hens at 26 weeks were randomly divided into 0, 1, 5 and 10 mg/kg.bw (body weight) DON daily for 6 weeks. The morphology of the duodenum, the expression of inflammation factors and tight junction proteins, and the diversity and abundance of microbiota were analyzed in different levels of DON treated to laying hens. The results demonstrated that the mucosal detachment and reduction of the villi number were presented in different DON treated groups with a dose-effect manner. Additionally, the genes expression of pro-inflammatory factors IL-1β, IL-8, TNF-α and anti-inflammatory factors IL-10 were increased or decreased at 5 and 10 mg/kg.bw DON groups, respectively. The levels of ZO-1 and claudin-1 expression were significantly decreased in 5 and 10 mg/kg.bw DON groups. Moreover, the alpha diversity including Chao, ACE and Shannon indices were all reduced in DON treated groups. At the phylum level, <i<Firmicutes</i< and <i<Actinobacteria</i< and <i<Bacteroidetes</i<, <i<Proteobacteria</i<, and <i<Spirochaetes</i< were decreased and increased in 10 mg/kg.bw DON group, respectively. At the genus levels, the relative abundance of <i<Clostridium</i< and <i<Lactobacillus</i< in 5 and 10 mg/kg.bw DON groups, and <i<Alkanindiges</i< and <i<Spirochaeta</i< in the 10 mg/kg.bw DON were significantly decreased and increased, respectively. Moreover, there were significant correlation between the expression of tight junction proteins and the relative abundance of <i<Lactobacillus</i< and <i<Succinispira.</i< These results indicated that DON exposure to the laying hens can induce the inflammation and disrupt intestinal tight junctions, suggesting that DON can directly damage barrier function, which may be closely related to the dysbiosis of intestinal microbiota.
abstract_unstemmed	Deoxynivalenol is one the of most common mycotoxins in cereals and grains and causes a serious health threat to poultry and farm animals. Our previous study found that DON decreased the production performance of laying hens. It has been reported that DON could exert significant toxic effects on the intestinal barrier and microbiota. However, whether the decline of laying performance is related to intestinal barrier damage, and the underlying mechanisms of DON induced intestine function injury remain largely unclear in laying hens. In this study, 80 Hy-line brown laying hens at 26 weeks were randomly divided into 0, 1, 5 and 10 mg/kg.bw (body weight) DON daily for 6 weeks. The morphology of the duodenum, the expression of inflammation factors and tight junction proteins, and the diversity and abundance of microbiota were analyzed in different levels of DON treated to laying hens. The results demonstrated that the mucosal detachment and reduction of the villi number were presented in different DON treated groups with a dose-effect manner. Additionally, the genes expression of pro-inflammatory factors IL-1β, IL-8, TNF-α and anti-inflammatory factors IL-10 were increased or decreased at 5 and 10 mg/kg.bw DON groups, respectively. The levels of ZO-1 and claudin-1 expression were significantly decreased in 5 and 10 mg/kg.bw DON groups. Moreover, the alpha diversity including Chao, ACE and Shannon indices were all reduced in DON treated groups. At the phylum level, <i<Firmicutes</i< and <i<Actinobacteria</i< and <i<Bacteroidetes</i<, <i<Proteobacteria</i<, and <i<Spirochaetes</i< were decreased and increased in 10 mg/kg.bw DON group, respectively. At the genus levels, the relative abundance of <i<Clostridium</i< and <i<Lactobacillus</i< in 5 and 10 mg/kg.bw DON groups, and <i<Alkanindiges</i< and <i<Spirochaeta</i< in the 10 mg/kg.bw DON were significantly decreased and increased, respectively. Moreover, there were significant correlation between the expression of tight junction proteins and the relative abundance of <i<Lactobacillus</i< and <i<Succinispira.</i< These results indicated that DON exposure to the laying hens can induce the inflammation and disrupt intestinal tight junctions, suggesting that DON can directly damage barrier function, which may be closely related to the dysbiosis of intestinal microbiota.
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container_issue	10, p 682
title_short	Possible Toxic Mechanisms of Deoxynivalenol (DON) Exposure to Intestinal Barrier Damage and Dysbiosis of the Gut Microbiota in Laying Hens
url	https://doi.org/10.3390/toxins14100682 https://doaj.org/article/74a3a0a5423943e9b256030b25ab08ab https://www.mdpi.com/2072-6651/14/10/682 https://doaj.org/toc/2072-6651
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Possible Toxic Mechanisms of Deoxynivalenol (DON) Exposure to Intestinal Barrier Damage and Dysbiosis of the Gut Microbiota in Laying Hens

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