Isolation of small extracellular vesicles from a drop of plasma via EXODUS and their fingerprint proteomics profiling by MALDI-TOF MS

Small extracellular vesicles (sEVs) in blood have emerged as the most promising biomarkers for clinical diagnostics and prognostics. However, isolation and identification of intact sEVs from blood are the major obstacles for basic research and clinical translations. Here, we report rapid isolation a...
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Autor*in:	Wen Ye [verfasserIn] Reguang Pan [verfasserIn] Ke-Qing Shi [verfasserIn] Hui-Ping Li [verfasserIn] Luke P. Lee [verfasserIn] Fei Liu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Small extracellular vesicles Plasma Label-free Rapid isolation Quantitative analysis MALDI-TOF MS

Übergeordnetes Werk:	In: Biosensors and Bioelectronics: X - Elsevier, 2021, 10(2022), Seite 100099-
Übergeordnetes Werk:	volume:10 ; year:2022 ; pages:100099-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.biosx.2021.100099

Katalog-ID:	DOAJ027880699

Internformat


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520			\|a Small extracellular vesicles (sEVs) in blood have emerged as the most promising biomarkers for clinical diagnostics and prognostics. However, isolation and identification of intact sEVs from blood are the major obstacles for basic research and clinical translations. Here, we report rapid isolation and sensitive detection of plasma sEVs by an integrative platform of sEV detection via the ultrafast-isolation system (EXODUS) and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). We can achieve label-free isolation of sEVs with relatively high recovery and purity by the EXODUS purification method from 20 μL of plasma, which was compared with polyethylene glycol-based precipitation and ultracentrifugation methods. We have profiled the fingerprints of the intact sEVs isolated from the different volumes of plasma using MALDI-TOF MS within 1 h. Further, we have evaluated the reproducibility and identified the metabolomic biomarkers of plasma sEVs via LC-ESI-MS/MS. We believe the combination of rapid EXODUS isolation and MALDI-TOF MS detection may serve as a clinical translation method for fast and high-throughput biomarker detection and screening.
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650		4	\|a Plasma
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650		4	\|a MALDI-TOF MS
653		0	\|a Biotechnology
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700	0		\|a Fei Liu \|e verfasserin \|4 aut
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912			\|a GBV_ILN_206
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912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
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912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4249
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912			\|a GBV_ILN_4334
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912			\|a GBV_ILN_4338
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author_variant	w y wy r p rp k q s kqs h p l hpl l p l lpl f l fl
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publishDate	2022
allfields	10.1016/j.biosx.2021.100099 doi (DE-627)DOAJ027880699 (DE-599)DOAJ22d1cce2f529435ba8bb1c6fdbba04e0 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Wen Ye verfasserin aut Isolation of small extracellular vesicles from a drop of plasma via EXODUS and their fingerprint proteomics profiling by MALDI-TOF MS 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Small extracellular vesicles (sEVs) in blood have emerged as the most promising biomarkers for clinical diagnostics and prognostics. However, isolation and identification of intact sEVs from blood are the major obstacles for basic research and clinical translations. Here, we report rapid isolation and sensitive detection of plasma sEVs by an integrative platform of sEV detection via the ultrafast-isolation system (EXODUS) and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). We can achieve label-free isolation of sEVs with relatively high recovery and purity by the EXODUS purification method from 20 μL of plasma, which was compared with polyethylene glycol-based precipitation and ultracentrifugation methods. We have profiled the fingerprints of the intact sEVs isolated from the different volumes of plasma using MALDI-TOF MS within 1 h. Further, we have evaluated the reproducibility and identified the metabolomic biomarkers of plasma sEVs via LC-ESI-MS/MS. We believe the combination of rapid EXODUS isolation and MALDI-TOF MS detection may serve as a clinical translation method for fast and high-throughput biomarker detection and screening. Small extracellular vesicles Plasma Label-free Rapid isolation Quantitative analysis MALDI-TOF MS Biotechnology Reguang Pan verfasserin aut Ke-Qing Shi verfasserin aut Hui-Ping Li verfasserin aut Luke P. Lee verfasserin aut Fei Liu verfasserin aut In Biosensors and Bioelectronics: X Elsevier, 2021 10(2022), Seite 100099- (DE-627)168360217X 25901370 nnns volume:10 year:2022 pages:100099- https://doi.org/10.1016/j.biosx.2021.100099 kostenfrei https://doaj.org/article/22d1cce2f529435ba8bb1c6fdbba04e0 kostenfrei http://www.sciencedirect.com/science/article/pii/S2590137021000352 kostenfrei https://doaj.org/toc/2590-1370 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 10 2022 100099-
spelling	10.1016/j.biosx.2021.100099 doi (DE-627)DOAJ027880699 (DE-599)DOAJ22d1cce2f529435ba8bb1c6fdbba04e0 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Wen Ye verfasserin aut Isolation of small extracellular vesicles from a drop of plasma via EXODUS and their fingerprint proteomics profiling by MALDI-TOF MS 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Small extracellular vesicles (sEVs) in blood have emerged as the most promising biomarkers for clinical diagnostics and prognostics. However, isolation and identification of intact sEVs from blood are the major obstacles for basic research and clinical translations. Here, we report rapid isolation and sensitive detection of plasma sEVs by an integrative platform of sEV detection via the ultrafast-isolation system (EXODUS) and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). We can achieve label-free isolation of sEVs with relatively high recovery and purity by the EXODUS purification method from 20 μL of plasma, which was compared with polyethylene glycol-based precipitation and ultracentrifugation methods. We have profiled the fingerprints of the intact sEVs isolated from the different volumes of plasma using MALDI-TOF MS within 1 h. Further, we have evaluated the reproducibility and identified the metabolomic biomarkers of plasma sEVs via LC-ESI-MS/MS. We believe the combination of rapid EXODUS isolation and MALDI-TOF MS detection may serve as a clinical translation method for fast and high-throughput biomarker detection and screening. Small extracellular vesicles Plasma Label-free Rapid isolation Quantitative analysis MALDI-TOF MS Biotechnology Reguang Pan verfasserin aut Ke-Qing Shi verfasserin aut Hui-Ping Li verfasserin aut Luke P. Lee verfasserin aut Fei Liu verfasserin aut In Biosensors and Bioelectronics: X Elsevier, 2021 10(2022), Seite 100099- (DE-627)168360217X 25901370 nnns volume:10 year:2022 pages:100099- https://doi.org/10.1016/j.biosx.2021.100099 kostenfrei https://doaj.org/article/22d1cce2f529435ba8bb1c6fdbba04e0 kostenfrei http://www.sciencedirect.com/science/article/pii/S2590137021000352 kostenfrei https://doaj.org/toc/2590-1370 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 10 2022 100099-
allfields_unstemmed	10.1016/j.biosx.2021.100099 doi (DE-627)DOAJ027880699 (DE-599)DOAJ22d1cce2f529435ba8bb1c6fdbba04e0 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Wen Ye verfasserin aut Isolation of small extracellular vesicles from a drop of plasma via EXODUS and their fingerprint proteomics profiling by MALDI-TOF MS 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Small extracellular vesicles (sEVs) in blood have emerged as the most promising biomarkers for clinical diagnostics and prognostics. However, isolation and identification of intact sEVs from blood are the major obstacles for basic research and clinical translations. Here, we report rapid isolation and sensitive detection of plasma sEVs by an integrative platform of sEV detection via the ultrafast-isolation system (EXODUS) and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). We can achieve label-free isolation of sEVs with relatively high recovery and purity by the EXODUS purification method from 20 μL of plasma, which was compared with polyethylene glycol-based precipitation and ultracentrifugation methods. We have profiled the fingerprints of the intact sEVs isolated from the different volumes of plasma using MALDI-TOF MS within 1 h. Further, we have evaluated the reproducibility and identified the metabolomic biomarkers of plasma sEVs via LC-ESI-MS/MS. We believe the combination of rapid EXODUS isolation and MALDI-TOF MS detection may serve as a clinical translation method for fast and high-throughput biomarker detection and screening. Small extracellular vesicles Plasma Label-free Rapid isolation Quantitative analysis MALDI-TOF MS Biotechnology Reguang Pan verfasserin aut Ke-Qing Shi verfasserin aut Hui-Ping Li verfasserin aut Luke P. Lee verfasserin aut Fei Liu verfasserin aut In Biosensors and Bioelectronics: X Elsevier, 2021 10(2022), Seite 100099- (DE-627)168360217X 25901370 nnns volume:10 year:2022 pages:100099- https://doi.org/10.1016/j.biosx.2021.100099 kostenfrei https://doaj.org/article/22d1cce2f529435ba8bb1c6fdbba04e0 kostenfrei http://www.sciencedirect.com/science/article/pii/S2590137021000352 kostenfrei https://doaj.org/toc/2590-1370 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 10 2022 100099-
allfieldsGer	10.1016/j.biosx.2021.100099 doi (DE-627)DOAJ027880699 (DE-599)DOAJ22d1cce2f529435ba8bb1c6fdbba04e0 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Wen Ye verfasserin aut Isolation of small extracellular vesicles from a drop of plasma via EXODUS and their fingerprint proteomics profiling by MALDI-TOF MS 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Small extracellular vesicles (sEVs) in blood have emerged as the most promising biomarkers for clinical diagnostics and prognostics. However, isolation and identification of intact sEVs from blood are the major obstacles for basic research and clinical translations. Here, we report rapid isolation and sensitive detection of plasma sEVs by an integrative platform of sEV detection via the ultrafast-isolation system (EXODUS) and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). We can achieve label-free isolation of sEVs with relatively high recovery and purity by the EXODUS purification method from 20 μL of plasma, which was compared with polyethylene glycol-based precipitation and ultracentrifugation methods. We have profiled the fingerprints of the intact sEVs isolated from the different volumes of plasma using MALDI-TOF MS within 1 h. Further, we have evaluated the reproducibility and identified the metabolomic biomarkers of plasma sEVs via LC-ESI-MS/MS. We believe the combination of rapid EXODUS isolation and MALDI-TOF MS detection may serve as a clinical translation method for fast and high-throughput biomarker detection and screening. Small extracellular vesicles Plasma Label-free Rapid isolation Quantitative analysis MALDI-TOF MS Biotechnology Reguang Pan verfasserin aut Ke-Qing Shi verfasserin aut Hui-Ping Li verfasserin aut Luke P. Lee verfasserin aut Fei Liu verfasserin aut In Biosensors and Bioelectronics: X Elsevier, 2021 10(2022), Seite 100099- (DE-627)168360217X 25901370 nnns volume:10 year:2022 pages:100099- https://doi.org/10.1016/j.biosx.2021.100099 kostenfrei https://doaj.org/article/22d1cce2f529435ba8bb1c6fdbba04e0 kostenfrei http://www.sciencedirect.com/science/article/pii/S2590137021000352 kostenfrei https://doaj.org/toc/2590-1370 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 10 2022 100099-
allfieldsSound	10.1016/j.biosx.2021.100099 doi (DE-627)DOAJ027880699 (DE-599)DOAJ22d1cce2f529435ba8bb1c6fdbba04e0 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Wen Ye verfasserin aut Isolation of small extracellular vesicles from a drop of plasma via EXODUS and their fingerprint proteomics profiling by MALDI-TOF MS 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Small extracellular vesicles (sEVs) in blood have emerged as the most promising biomarkers for clinical diagnostics and prognostics. However, isolation and identification of intact sEVs from blood are the major obstacles for basic research and clinical translations. Here, we report rapid isolation and sensitive detection of plasma sEVs by an integrative platform of sEV detection via the ultrafast-isolation system (EXODUS) and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). We can achieve label-free isolation of sEVs with relatively high recovery and purity by the EXODUS purification method from 20 μL of plasma, which was compared with polyethylene glycol-based precipitation and ultracentrifugation methods. We have profiled the fingerprints of the intact sEVs isolated from the different volumes of plasma using MALDI-TOF MS within 1 h. Further, we have evaluated the reproducibility and identified the metabolomic biomarkers of plasma sEVs via LC-ESI-MS/MS. We believe the combination of rapid EXODUS isolation and MALDI-TOF MS detection may serve as a clinical translation method for fast and high-throughput biomarker detection and screening. Small extracellular vesicles Plasma Label-free Rapid isolation Quantitative analysis MALDI-TOF MS Biotechnology Reguang Pan verfasserin aut Ke-Qing Shi verfasserin aut Hui-Ping Li verfasserin aut Luke P. Lee verfasserin aut Fei Liu verfasserin aut In Biosensors and Bioelectronics: X Elsevier, 2021 10(2022), Seite 100099- (DE-627)168360217X 25901370 nnns volume:10 year:2022 pages:100099- https://doi.org/10.1016/j.biosx.2021.100099 kostenfrei https://doaj.org/article/22d1cce2f529435ba8bb1c6fdbba04e0 kostenfrei http://www.sciencedirect.com/science/article/pii/S2590137021000352 kostenfrei https://doaj.org/toc/2590-1370 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 10 2022 100099-
language	English
source	In Biosensors and Bioelectronics: X 10(2022), Seite 100099- volume:10 year:2022 pages:100099-
sourceStr	In Biosensors and Bioelectronics: X 10(2022), Seite 100099- volume:10 year:2022 pages:100099-
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Small extracellular vesicles Plasma Label-free Rapid isolation Quantitative analysis MALDI-TOF MS Biotechnology
isfreeaccess_bool	true
container_title	Biosensors and Bioelectronics: X
authorswithroles_txt_mv	Wen Ye @@aut@@ Reguang Pan @@aut@@ Ke-Qing Shi @@aut@@ Hui-Ping Li @@aut@@ Luke P. Lee @@aut@@ Fei Liu @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	168360217X
id	DOAJ027880699
language_de	englisch
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callnumber-first	T - Technology
author	Wen Ye
spellingShingle	Wen Ye misc TP248.13-248.65 misc Small extracellular vesicles misc Plasma misc Label-free misc Rapid isolation misc Quantitative analysis misc MALDI-TOF MS misc Biotechnology Isolation of small extracellular vesicles from a drop of plasma via EXODUS and their fingerprint proteomics profiling by MALDI-TOF MS
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topic_title	TP248.13-248.65 Isolation of small extracellular vesicles from a drop of plasma via EXODUS and their fingerprint proteomics profiling by MALDI-TOF MS Small extracellular vesicles Plasma Label-free Rapid isolation Quantitative analysis MALDI-TOF MS
topic	misc TP248.13-248.65 misc Small extracellular vesicles misc Plasma misc Label-free misc Rapid isolation misc Quantitative analysis misc MALDI-TOF MS misc Biotechnology
topic_unstemmed	misc TP248.13-248.65 misc Small extracellular vesicles misc Plasma misc Label-free misc Rapid isolation misc Quantitative analysis misc MALDI-TOF MS misc Biotechnology
topic_browse	misc TP248.13-248.65 misc Small extracellular vesicles misc Plasma misc Label-free misc Rapid isolation misc Quantitative analysis misc MALDI-TOF MS misc Biotechnology
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title	Isolation of small extracellular vesicles from a drop of plasma via EXODUS and their fingerprint proteomics profiling by MALDI-TOF MS
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title_full	Isolation of small extracellular vesicles from a drop of plasma via EXODUS and their fingerprint proteomics profiling by MALDI-TOF MS
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author_browse	Wen Ye Reguang Pan Ke-Qing Shi Hui-Ping Li Luke P. Lee Fei Liu
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title_sort	isolation of small extracellular vesicles from a drop of plasma via exodus and their fingerprint proteomics profiling by maldi-tof ms
callnumber	TP248.13-248.65
title_auth	Isolation of small extracellular vesicles from a drop of plasma via EXODUS and their fingerprint proteomics profiling by MALDI-TOF MS
abstract	Small extracellular vesicles (sEVs) in blood have emerged as the most promising biomarkers for clinical diagnostics and prognostics. However, isolation and identification of intact sEVs from blood are the major obstacles for basic research and clinical translations. Here, we report rapid isolation and sensitive detection of plasma sEVs by an integrative platform of sEV detection via the ultrafast-isolation system (EXODUS) and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). We can achieve label-free isolation of sEVs with relatively high recovery and purity by the EXODUS purification method from 20 μL of plasma, which was compared with polyethylene glycol-based precipitation and ultracentrifugation methods. We have profiled the fingerprints of the intact sEVs isolated from the different volumes of plasma using MALDI-TOF MS within 1 h. Further, we have evaluated the reproducibility and identified the metabolomic biomarkers of plasma sEVs via LC-ESI-MS/MS. We believe the combination of rapid EXODUS isolation and MALDI-TOF MS detection may serve as a clinical translation method for fast and high-throughput biomarker detection and screening.
abstractGer	Small extracellular vesicles (sEVs) in blood have emerged as the most promising biomarkers for clinical diagnostics and prognostics. However, isolation and identification of intact sEVs from blood are the major obstacles for basic research and clinical translations. Here, we report rapid isolation and sensitive detection of plasma sEVs by an integrative platform of sEV detection via the ultrafast-isolation system (EXODUS) and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). We can achieve label-free isolation of sEVs with relatively high recovery and purity by the EXODUS purification method from 20 μL of plasma, which was compared with polyethylene glycol-based precipitation and ultracentrifugation methods. We have profiled the fingerprints of the intact sEVs isolated from the different volumes of plasma using MALDI-TOF MS within 1 h. Further, we have evaluated the reproducibility and identified the metabolomic biomarkers of plasma sEVs via LC-ESI-MS/MS. We believe the combination of rapid EXODUS isolation and MALDI-TOF MS detection may serve as a clinical translation method for fast and high-throughput biomarker detection and screening.
abstract_unstemmed	Small extracellular vesicles (sEVs) in blood have emerged as the most promising biomarkers for clinical diagnostics and prognostics. However, isolation and identification of intact sEVs from blood are the major obstacles for basic research and clinical translations. Here, we report rapid isolation and sensitive detection of plasma sEVs by an integrative platform of sEV detection via the ultrafast-isolation system (EXODUS) and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). We can achieve label-free isolation of sEVs with relatively high recovery and purity by the EXODUS purification method from 20 μL of plasma, which was compared with polyethylene glycol-based precipitation and ultracentrifugation methods. We have profiled the fingerprints of the intact sEVs isolated from the different volumes of plasma using MALDI-TOF MS within 1 h. Further, we have evaluated the reproducibility and identified the metabolomic biomarkers of plasma sEVs via LC-ESI-MS/MS. We believe the combination of rapid EXODUS isolation and MALDI-TOF MS detection may serve as a clinical translation method for fast and high-throughput biomarker detection and screening.
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title_short	Isolation of small extracellular vesicles from a drop of plasma via EXODUS and their fingerprint proteomics profiling by MALDI-TOF MS
url	https://doi.org/10.1016/j.biosx.2021.100099 https://doaj.org/article/22d1cce2f529435ba8bb1c6fdbba04e0 http://www.sciencedirect.com/science/article/pii/S2590137021000352 https://doaj.org/toc/2590-1370
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up_date	2024-07-03T14:32:36.475Z
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Isolation of small extracellular vesicles from a drop of plasma via EXODUS and their fingerprint proteomics profiling by MALDI-TOF MS

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