CsPAO2 Improves Salt Tolerance of Cucumber through the Interaction with CsPSA3 by Affecting Photosynthesis and Polyamine Conversion

Polyamine oxidases (PAOs) are key enzymes in polyamine metabolism and are related to the tolerance of plants to abiotic stresses. In this study, overexpression of cucumber (<i<Cucumis sativus</i< L.) <i<PAO2</i< (<i<CsPAO2</i<) in Arabidopsis resulted in increased...
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Autor*in:	Jianqiang Wu [verfasserIn] Mengliang Zhu [verfasserIn] Weikang Liu [verfasserIn] Mohammad Shah Jahan [verfasserIn] Qinsheng Gu [verfasserIn] Sheng Shu [verfasserIn] Jin Sun [verfasserIn] Shirong Guo [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	cucumber salt stress protein interaction photosynthesis polyamine

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 23(2022), 20, p 12413
Übergeordnetes Werk:	volume:23 ; year:2022 ; number:20, p 12413

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms232012413

Katalog-ID:	DOAJ027903842

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520			\|a Polyamine oxidases (PAOs) are key enzymes in polyamine metabolism and are related to the tolerance of plants to abiotic stresses. In this study, overexpression of cucumber (<i<Cucumis sativus</i< L.) <i<PAO2</i< (<i<CsPAO2</i<) in Arabidopsis resulted in increased activity of the antioxidant enzyme and accelerated conversion from Put to Spd and Spm, while malondialdehyde content (MDA) and electrolyte leakage (EL) was decreased when compared with wild type, leading to enhanced plant growth under salt stress. Photosystem Ⅰ assembly 3 in cucumber (CsPSA3) was revealed as an interacting protein of CsPAO2 by screening yeast two-hybrid library combined with in vitro and in vivo methods. Then, <i<CsPAO2</i< and <i<CsPSA3</i< were silenced in cucumber via virus-mediated gene silencing (VIGS) with pV190 as the empty vector. Under salt stress, net photosynthetic rate (Pn) and transpiration rate (Tr) of <i<CsPAO2</i<-silencing plants were lower than pV190-silencing plants, and EL in root was higher than pV190-silencing plants, indicating that <i<CsPAO2</i<-silencing plants suffered more serious salt stress damage. However, photosynthetic parameters of <i<CsPSA3</i<-silencing plants were all higher than those of <i<CsPAO2</i< and pV190-silencing plants, thereby enhancing the photosynthesis process. Moreover, <i<CsPSA3</i< silencing reduced the EL in both leaves and roots when compared with <i<CsPAO2</i<-silencing plants, but the EL only in leaves was significantly lower than the other two gene-silencing plants, and conversion from Put to Spd and Spm in leaf was also promoted, suggesting that CsPSA3 interacts with CsPAO2 in leaves to participate in the regulation of salt tolerance through photosynthesis and polyamine conversion.
650		4	\|a cucumber
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700	0		\|a Shirong Guo \|e verfasserin \|4 aut
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allfields	10.3390/ijms232012413 doi (DE-627)DOAJ027903842 (DE-599)DOAJ475267b0a38f4476a2d6f8969717c915 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jianqiang Wu verfasserin aut CsPAO2 Improves Salt Tolerance of Cucumber through the Interaction with CsPSA3 by Affecting Photosynthesis and Polyamine Conversion 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Polyamine oxidases (PAOs) are key enzymes in polyamine metabolism and are related to the tolerance of plants to abiotic stresses. In this study, overexpression of cucumber (<i<Cucumis sativus</i< L.) <i<PAO2</i< (<i<CsPAO2</i<) in Arabidopsis resulted in increased activity of the antioxidant enzyme and accelerated conversion from Put to Spd and Spm, while malondialdehyde content (MDA) and electrolyte leakage (EL) was decreased when compared with wild type, leading to enhanced plant growth under salt stress. Photosystem Ⅰ assembly 3 in cucumber (CsPSA3) was revealed as an interacting protein of CsPAO2 by screening yeast two-hybrid library combined with in vitro and in vivo methods. Then, <i<CsPAO2</i< and <i<CsPSA3</i< were silenced in cucumber via virus-mediated gene silencing (VIGS) with pV190 as the empty vector. Under salt stress, net photosynthetic rate (Pn) and transpiration rate (Tr) of <i<CsPAO2</i<-silencing plants were lower than pV190-silencing plants, and EL in root was higher than pV190-silencing plants, indicating that <i<CsPAO2</i<-silencing plants suffered more serious salt stress damage. However, photosynthetic parameters of <i<CsPSA3</i<-silencing plants were all higher than those of <i<CsPAO2</i< and pV190-silencing plants, thereby enhancing the photosynthesis process. Moreover, <i<CsPSA3</i< silencing reduced the EL in both leaves and roots when compared with <i<CsPAO2</i<-silencing plants, but the EL only in leaves was significantly lower than the other two gene-silencing plants, and conversion from Put to Spd and Spm in leaf was also promoted, suggesting that CsPSA3 interacts with CsPAO2 in leaves to participate in the regulation of salt tolerance through photosynthesis and polyamine conversion. cucumber salt stress protein interaction photosynthesis polyamine Biology (General) Chemistry Mengliang Zhu verfasserin aut Weikang Liu verfasserin aut Mohammad Shah Jahan verfasserin aut Qinsheng Gu verfasserin aut Sheng Shu verfasserin aut Jin Sun verfasserin aut Shirong Guo verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 20, p 12413 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:20, p 12413 https://doi.org/10.3390/ijms232012413 kostenfrei https://doaj.org/article/475267b0a38f4476a2d6f8969717c915 kostenfrei https://www.mdpi.com/1422-0067/23/20/12413 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 20, p 12413
spelling	10.3390/ijms232012413 doi (DE-627)DOAJ027903842 (DE-599)DOAJ475267b0a38f4476a2d6f8969717c915 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jianqiang Wu verfasserin aut CsPAO2 Improves Salt Tolerance of Cucumber through the Interaction with CsPSA3 by Affecting Photosynthesis and Polyamine Conversion 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Polyamine oxidases (PAOs) are key enzymes in polyamine metabolism and are related to the tolerance of plants to abiotic stresses. In this study, overexpression of cucumber (<i<Cucumis sativus</i< L.) <i<PAO2</i< (<i<CsPAO2</i<) in Arabidopsis resulted in increased activity of the antioxidant enzyme and accelerated conversion from Put to Spd and Spm, while malondialdehyde content (MDA) and electrolyte leakage (EL) was decreased when compared with wild type, leading to enhanced plant growth under salt stress. Photosystem Ⅰ assembly 3 in cucumber (CsPSA3) was revealed as an interacting protein of CsPAO2 by screening yeast two-hybrid library combined with in vitro and in vivo methods. Then, <i<CsPAO2</i< and <i<CsPSA3</i< were silenced in cucumber via virus-mediated gene silencing (VIGS) with pV190 as the empty vector. Under salt stress, net photosynthetic rate (Pn) and transpiration rate (Tr) of <i<CsPAO2</i<-silencing plants were lower than pV190-silencing plants, and EL in root was higher than pV190-silencing plants, indicating that <i<CsPAO2</i<-silencing plants suffered more serious salt stress damage. However, photosynthetic parameters of <i<CsPSA3</i<-silencing plants were all higher than those of <i<CsPAO2</i< and pV190-silencing plants, thereby enhancing the photosynthesis process. Moreover, <i<CsPSA3</i< silencing reduced the EL in both leaves and roots when compared with <i<CsPAO2</i<-silencing plants, but the EL only in leaves was significantly lower than the other two gene-silencing plants, and conversion from Put to Spd and Spm in leaf was also promoted, suggesting that CsPSA3 interacts with CsPAO2 in leaves to participate in the regulation of salt tolerance through photosynthesis and polyamine conversion. cucumber salt stress protein interaction photosynthesis polyamine Biology (General) Chemistry Mengliang Zhu verfasserin aut Weikang Liu verfasserin aut Mohammad Shah Jahan verfasserin aut Qinsheng Gu verfasserin aut Sheng Shu verfasserin aut Jin Sun verfasserin aut Shirong Guo verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 20, p 12413 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:20, p 12413 https://doi.org/10.3390/ijms232012413 kostenfrei https://doaj.org/article/475267b0a38f4476a2d6f8969717c915 kostenfrei https://www.mdpi.com/1422-0067/23/20/12413 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 20, p 12413
allfields_unstemmed	10.3390/ijms232012413 doi (DE-627)DOAJ027903842 (DE-599)DOAJ475267b0a38f4476a2d6f8969717c915 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jianqiang Wu verfasserin aut CsPAO2 Improves Salt Tolerance of Cucumber through the Interaction with CsPSA3 by Affecting Photosynthesis and Polyamine Conversion 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Polyamine oxidases (PAOs) are key enzymes in polyamine metabolism and are related to the tolerance of plants to abiotic stresses. In this study, overexpression of cucumber (<i<Cucumis sativus</i< L.) <i<PAO2</i< (<i<CsPAO2</i<) in Arabidopsis resulted in increased activity of the antioxidant enzyme and accelerated conversion from Put to Spd and Spm, while malondialdehyde content (MDA) and electrolyte leakage (EL) was decreased when compared with wild type, leading to enhanced plant growth under salt stress. Photosystem Ⅰ assembly 3 in cucumber (CsPSA3) was revealed as an interacting protein of CsPAO2 by screening yeast two-hybrid library combined with in vitro and in vivo methods. Then, <i<CsPAO2</i< and <i<CsPSA3</i< were silenced in cucumber via virus-mediated gene silencing (VIGS) with pV190 as the empty vector. Under salt stress, net photosynthetic rate (Pn) and transpiration rate (Tr) of <i<CsPAO2</i<-silencing plants were lower than pV190-silencing plants, and EL in root was higher than pV190-silencing plants, indicating that <i<CsPAO2</i<-silencing plants suffered more serious salt stress damage. However, photosynthetic parameters of <i<CsPSA3</i<-silencing plants were all higher than those of <i<CsPAO2</i< and pV190-silencing plants, thereby enhancing the photosynthesis process. Moreover, <i<CsPSA3</i< silencing reduced the EL in both leaves and roots when compared with <i<CsPAO2</i<-silencing plants, but the EL only in leaves was significantly lower than the other two gene-silencing plants, and conversion from Put to Spd and Spm in leaf was also promoted, suggesting that CsPSA3 interacts with CsPAO2 in leaves to participate in the regulation of salt tolerance through photosynthesis and polyamine conversion. cucumber salt stress protein interaction photosynthesis polyamine Biology (General) Chemistry Mengliang Zhu verfasserin aut Weikang Liu verfasserin aut Mohammad Shah Jahan verfasserin aut Qinsheng Gu verfasserin aut Sheng Shu verfasserin aut Jin Sun verfasserin aut Shirong Guo verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 20, p 12413 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:20, p 12413 https://doi.org/10.3390/ijms232012413 kostenfrei https://doaj.org/article/475267b0a38f4476a2d6f8969717c915 kostenfrei https://www.mdpi.com/1422-0067/23/20/12413 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 20, p 12413
allfieldsGer	10.3390/ijms232012413 doi (DE-627)DOAJ027903842 (DE-599)DOAJ475267b0a38f4476a2d6f8969717c915 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jianqiang Wu verfasserin aut CsPAO2 Improves Salt Tolerance of Cucumber through the Interaction with CsPSA3 by Affecting Photosynthesis and Polyamine Conversion 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Polyamine oxidases (PAOs) are key enzymes in polyamine metabolism and are related to the tolerance of plants to abiotic stresses. In this study, overexpression of cucumber (<i<Cucumis sativus</i< L.) <i<PAO2</i< (<i<CsPAO2</i<) in Arabidopsis resulted in increased activity of the antioxidant enzyme and accelerated conversion from Put to Spd and Spm, while malondialdehyde content (MDA) and electrolyte leakage (EL) was decreased when compared with wild type, leading to enhanced plant growth under salt stress. Photosystem Ⅰ assembly 3 in cucumber (CsPSA3) was revealed as an interacting protein of CsPAO2 by screening yeast two-hybrid library combined with in vitro and in vivo methods. Then, <i<CsPAO2</i< and <i<CsPSA3</i< were silenced in cucumber via virus-mediated gene silencing (VIGS) with pV190 as the empty vector. Under salt stress, net photosynthetic rate (Pn) and transpiration rate (Tr) of <i<CsPAO2</i<-silencing plants were lower than pV190-silencing plants, and EL in root was higher than pV190-silencing plants, indicating that <i<CsPAO2</i<-silencing plants suffered more serious salt stress damage. However, photosynthetic parameters of <i<CsPSA3</i<-silencing plants were all higher than those of <i<CsPAO2</i< and pV190-silencing plants, thereby enhancing the photosynthesis process. Moreover, <i<CsPSA3</i< silencing reduced the EL in both leaves and roots when compared with <i<CsPAO2</i<-silencing plants, but the EL only in leaves was significantly lower than the other two gene-silencing plants, and conversion from Put to Spd and Spm in leaf was also promoted, suggesting that CsPSA3 interacts with CsPAO2 in leaves to participate in the regulation of salt tolerance through photosynthesis and polyamine conversion. cucumber salt stress protein interaction photosynthesis polyamine Biology (General) Chemistry Mengliang Zhu verfasserin aut Weikang Liu verfasserin aut Mohammad Shah Jahan verfasserin aut Qinsheng Gu verfasserin aut Sheng Shu verfasserin aut Jin Sun verfasserin aut Shirong Guo verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 20, p 12413 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:20, p 12413 https://doi.org/10.3390/ijms232012413 kostenfrei https://doaj.org/article/475267b0a38f4476a2d6f8969717c915 kostenfrei https://www.mdpi.com/1422-0067/23/20/12413 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 20, p 12413
allfieldsSound	10.3390/ijms232012413 doi (DE-627)DOAJ027903842 (DE-599)DOAJ475267b0a38f4476a2d6f8969717c915 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Jianqiang Wu verfasserin aut CsPAO2 Improves Salt Tolerance of Cucumber through the Interaction with CsPSA3 by Affecting Photosynthesis and Polyamine Conversion 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Polyamine oxidases (PAOs) are key enzymes in polyamine metabolism and are related to the tolerance of plants to abiotic stresses. In this study, overexpression of cucumber (<i<Cucumis sativus</i< L.) <i<PAO2</i< (<i<CsPAO2</i<) in Arabidopsis resulted in increased activity of the antioxidant enzyme and accelerated conversion from Put to Spd and Spm, while malondialdehyde content (MDA) and electrolyte leakage (EL) was decreased when compared with wild type, leading to enhanced plant growth under salt stress. Photosystem Ⅰ assembly 3 in cucumber (CsPSA3) was revealed as an interacting protein of CsPAO2 by screening yeast two-hybrid library combined with in vitro and in vivo methods. Then, <i<CsPAO2</i< and <i<CsPSA3</i< were silenced in cucumber via virus-mediated gene silencing (VIGS) with pV190 as the empty vector. Under salt stress, net photosynthetic rate (Pn) and transpiration rate (Tr) of <i<CsPAO2</i<-silencing plants were lower than pV190-silencing plants, and EL in root was higher than pV190-silencing plants, indicating that <i<CsPAO2</i<-silencing plants suffered more serious salt stress damage. However, photosynthetic parameters of <i<CsPSA3</i<-silencing plants were all higher than those of <i<CsPAO2</i< and pV190-silencing plants, thereby enhancing the photosynthesis process. Moreover, <i<CsPSA3</i< silencing reduced the EL in both leaves and roots when compared with <i<CsPAO2</i<-silencing plants, but the EL only in leaves was significantly lower than the other two gene-silencing plants, and conversion from Put to Spd and Spm in leaf was also promoted, suggesting that CsPSA3 interacts with CsPAO2 in leaves to participate in the regulation of salt tolerance through photosynthesis and polyamine conversion. cucumber salt stress protein interaction photosynthesis polyamine Biology (General) Chemistry Mengliang Zhu verfasserin aut Weikang Liu verfasserin aut Mohammad Shah Jahan verfasserin aut Qinsheng Gu verfasserin aut Sheng Shu verfasserin aut Jin Sun verfasserin aut Shirong Guo verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 20, p 12413 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:20, p 12413 https://doi.org/10.3390/ijms232012413 kostenfrei https://doaj.org/article/475267b0a38f4476a2d6f8969717c915 kostenfrei https://www.mdpi.com/1422-0067/23/20/12413 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 20, p 12413
language	English
source	In International Journal of Molecular Sciences 23(2022), 20, p 12413 volume:23 year:2022 number:20, p 12413
sourceStr	In International Journal of Molecular Sciences 23(2022), 20, p 12413 volume:23 year:2022 number:20, p 12413
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	cucumber salt stress protein interaction photosynthesis polyamine Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Jianqiang Wu @@aut@@ Mengliang Zhu @@aut@@ Weikang Liu @@aut@@ Mohammad Shah Jahan @@aut@@ Qinsheng Gu @@aut@@ Sheng Shu @@aut@@ Jin Sun @@aut@@ Shirong Guo @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ027903842
language_de	englisch
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callnumber-first	Q - Science
author	Jianqiang Wu
spellingShingle	Jianqiang Wu misc QH301-705.5 misc QD1-999 misc cucumber misc salt stress misc protein interaction misc photosynthesis misc polyamine misc Biology (General) misc Chemistry CsPAO2 Improves Salt Tolerance of Cucumber through the Interaction with CsPSA3 by Affecting Photosynthesis and Polyamine Conversion
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topic_title	QH301-705.5 QD1-999 CsPAO2 Improves Salt Tolerance of Cucumber through the Interaction with CsPSA3 by Affecting Photosynthesis and Polyamine Conversion cucumber salt stress protein interaction photosynthesis polyamine
topic	misc QH301-705.5 misc QD1-999 misc cucumber misc salt stress misc protein interaction misc photosynthesis misc polyamine misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc cucumber misc salt stress misc protein interaction misc photosynthesis misc polyamine misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc cucumber misc salt stress misc protein interaction misc photosynthesis misc polyamine misc Biology (General) misc Chemistry
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title	CsPAO2 Improves Salt Tolerance of Cucumber through the Interaction with CsPSA3 by Affecting Photosynthesis and Polyamine Conversion
ctrlnum	(DE-627)DOAJ027903842 (DE-599)DOAJ475267b0a38f4476a2d6f8969717c915
title_full	CsPAO2 Improves Salt Tolerance of Cucumber through the Interaction with CsPSA3 by Affecting Photosynthesis and Polyamine Conversion
author_sort	Jianqiang Wu
journal	International Journal of Molecular Sciences
journalStr	International Journal of Molecular Sciences
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author_browse	Jianqiang Wu Mengliang Zhu Weikang Liu Mohammad Shah Jahan Qinsheng Gu Sheng Shu Jin Sun Shirong Guo
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doi_str_mv	10.3390/ijms232012413
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title_sort	cspao2 improves salt tolerance of cucumber through the interaction with cspsa3 by affecting photosynthesis and polyamine conversion
callnumber	QH301-705.5
title_auth	CsPAO2 Improves Salt Tolerance of Cucumber through the Interaction with CsPSA3 by Affecting Photosynthesis and Polyamine Conversion
abstract	Polyamine oxidases (PAOs) are key enzymes in polyamine metabolism and are related to the tolerance of plants to abiotic stresses. In this study, overexpression of cucumber (<i<Cucumis sativus</i< L.) <i<PAO2</i< (<i<CsPAO2</i<) in Arabidopsis resulted in increased activity of the antioxidant enzyme and accelerated conversion from Put to Spd and Spm, while malondialdehyde content (MDA) and electrolyte leakage (EL) was decreased when compared with wild type, leading to enhanced plant growth under salt stress. Photosystem Ⅰ assembly 3 in cucumber (CsPSA3) was revealed as an interacting protein of CsPAO2 by screening yeast two-hybrid library combined with in vitro and in vivo methods. Then, <i<CsPAO2</i< and <i<CsPSA3</i< were silenced in cucumber via virus-mediated gene silencing (VIGS) with pV190 as the empty vector. Under salt stress, net photosynthetic rate (Pn) and transpiration rate (Tr) of <i<CsPAO2</i<-silencing plants were lower than pV190-silencing plants, and EL in root was higher than pV190-silencing plants, indicating that <i<CsPAO2</i<-silencing plants suffered more serious salt stress damage. However, photosynthetic parameters of <i<CsPSA3</i<-silencing plants were all higher than those of <i<CsPAO2</i< and pV190-silencing plants, thereby enhancing the photosynthesis process. Moreover, <i<CsPSA3</i< silencing reduced the EL in both leaves and roots when compared with <i<CsPAO2</i<-silencing plants, but the EL only in leaves was significantly lower than the other two gene-silencing plants, and conversion from Put to Spd and Spm in leaf was also promoted, suggesting that CsPSA3 interacts with CsPAO2 in leaves to participate in the regulation of salt tolerance through photosynthesis and polyamine conversion.
abstractGer	Polyamine oxidases (PAOs) are key enzymes in polyamine metabolism and are related to the tolerance of plants to abiotic stresses. In this study, overexpression of cucumber (<i<Cucumis sativus</i< L.) <i<PAO2</i< (<i<CsPAO2</i<) in Arabidopsis resulted in increased activity of the antioxidant enzyme and accelerated conversion from Put to Spd and Spm, while malondialdehyde content (MDA) and electrolyte leakage (EL) was decreased when compared with wild type, leading to enhanced plant growth under salt stress. Photosystem Ⅰ assembly 3 in cucumber (CsPSA3) was revealed as an interacting protein of CsPAO2 by screening yeast two-hybrid library combined with in vitro and in vivo methods. Then, <i<CsPAO2</i< and <i<CsPSA3</i< were silenced in cucumber via virus-mediated gene silencing (VIGS) with pV190 as the empty vector. Under salt stress, net photosynthetic rate (Pn) and transpiration rate (Tr) of <i<CsPAO2</i<-silencing plants were lower than pV190-silencing plants, and EL in root was higher than pV190-silencing plants, indicating that <i<CsPAO2</i<-silencing plants suffered more serious salt stress damage. However, photosynthetic parameters of <i<CsPSA3</i<-silencing plants were all higher than those of <i<CsPAO2</i< and pV190-silencing plants, thereby enhancing the photosynthesis process. Moreover, <i<CsPSA3</i< silencing reduced the EL in both leaves and roots when compared with <i<CsPAO2</i<-silencing plants, but the EL only in leaves was significantly lower than the other two gene-silencing plants, and conversion from Put to Spd and Spm in leaf was also promoted, suggesting that CsPSA3 interacts with CsPAO2 in leaves to participate in the regulation of salt tolerance through photosynthesis and polyamine conversion.
abstract_unstemmed	Polyamine oxidases (PAOs) are key enzymes in polyamine metabolism and are related to the tolerance of plants to abiotic stresses. In this study, overexpression of cucumber (<i<Cucumis sativus</i< L.) <i<PAO2</i< (<i<CsPAO2</i<) in Arabidopsis resulted in increased activity of the antioxidant enzyme and accelerated conversion from Put to Spd and Spm, while malondialdehyde content (MDA) and electrolyte leakage (EL) was decreased when compared with wild type, leading to enhanced plant growth under salt stress. Photosystem Ⅰ assembly 3 in cucumber (CsPSA3) was revealed as an interacting protein of CsPAO2 by screening yeast two-hybrid library combined with in vitro and in vivo methods. Then, <i<CsPAO2</i< and <i<CsPSA3</i< were silenced in cucumber via virus-mediated gene silencing (VIGS) with pV190 as the empty vector. Under salt stress, net photosynthetic rate (Pn) and transpiration rate (Tr) of <i<CsPAO2</i<-silencing plants were lower than pV190-silencing plants, and EL in root was higher than pV190-silencing plants, indicating that <i<CsPAO2</i<-silencing plants suffered more serious salt stress damage. However, photosynthetic parameters of <i<CsPSA3</i<-silencing plants were all higher than those of <i<CsPAO2</i< and pV190-silencing plants, thereby enhancing the photosynthesis process. Moreover, <i<CsPSA3</i< silencing reduced the EL in both leaves and roots when compared with <i<CsPAO2</i<-silencing plants, but the EL only in leaves was significantly lower than the other two gene-silencing plants, and conversion from Put to Spd and Spm in leaf was also promoted, suggesting that CsPSA3 interacts with CsPAO2 in leaves to participate in the regulation of salt tolerance through photosynthesis and polyamine conversion.
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title_short	CsPAO2 Improves Salt Tolerance of Cucumber through the Interaction with CsPSA3 by Affecting Photosynthesis and Polyamine Conversion
url	https://doi.org/10.3390/ijms232012413 https://doaj.org/article/475267b0a38f4476a2d6f8969717c915 https://www.mdpi.com/1422-0067/23/20/12413 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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CsPAO2 Improves Salt Tolerance of Cucumber through the Interaction with CsPSA3 by Affecting Photosynthesis and Polyamine Conversion

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