Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China

Abstract Vibrio vulnificus is a pathogenic bacterium that causes disease in marine fish, affecting fish farming and human health worldwide. In May 2021, in the Bohai Bay region, a disease broke out in commercially farmed pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus),...
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Autor*in:	Zun Wu [verfasserIn] Yating Wu [verfasserIn] Haofeng Gao [verfasserIn] Xuexin He [verfasserIn] Qiang Yao [verfasserIn] Zhanglei Yang [verfasserIn] Jinyi Zhou [verfasserIn] Linting Ji [verfasserIn] Jinwei Gao [verfasserIn] Xuying Jia [verfasserIn] Yong Dou [verfasserIn] Xiaoyu Wang [verfasserIn] Peng Shao [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Vibrio vulnificus Pearl gentian grouper Virulence genes Antimicrobial susceptibility Whole-genome sequencing

Übergeordnetes Werk:	In: BMC Microbiology - BMC, 2003, 22(2022), 1, Seite 15
Übergeordnetes Werk:	volume:22 ; year:2022 ; number:1 ; pages:15

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/s12866-022-02610-1

Katalog-ID:	DOAJ028154525

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520			\|a Abstract Vibrio vulnificus is a pathogenic bacterium that causes disease in marine fish, affecting fish farming and human health worldwide. In May 2021, in the Bohai Bay region, a disease broke out in commercially farmed pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus), causing huge economic losses. The diseased fish had skin lesions, water accumulation in their abdomens, and showed tissue and organ damage. V. vulnificus biotype 2 has been reported in eels and other marine fish, but it is less reported in pearl gentian grouper. In this study, the pathogenic strain isolated from diseased fish was identified as V. vulnificus EPL 0201 biotype 2 on the basis of physiological and biochemical characteristics and the results of 16S rRNA gene and gyrB sequencing, virulence gene detection, and recursive infection experiments. To gain a comprehensive understanding of the pathogenicity and drug resistance of this strain, whole-genome sequencing was performed. Whole-genome analysis showed that the gene map of this strain was complete. The Virulence Factor Database annotation results showed that this strain had the key virulence factor genes vvhA and rtxA, which cause host disease. In addition, this strain had genes conferring resistance against cephalosporins, aminoglycosides, tetracyclines, and sulfonamides. Antimicrobial susceptibility testing confirmed the presence of these resistance genes identified in the genome. The results of this study show that V. vulnificus EPL 0201 biotype 2 is a multi-drug resistant strain with high pathogenicity.
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allfields	10.1186/s12866-022-02610-1 doi (DE-627)DOAJ028154525 (DE-599)DOAJ479482bb142a4155ba2dba90002ae00f DE-627 ger DE-627 rakwb eng QR1-502 Zun Wu verfasserin aut Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Vibrio vulnificus is a pathogenic bacterium that causes disease in marine fish, affecting fish farming and human health worldwide. In May 2021, in the Bohai Bay region, a disease broke out in commercially farmed pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus), causing huge economic losses. The diseased fish had skin lesions, water accumulation in their abdomens, and showed tissue and organ damage. V. vulnificus biotype 2 has been reported in eels and other marine fish, but it is less reported in pearl gentian grouper. In this study, the pathogenic strain isolated from diseased fish was identified as V. vulnificus EPL 0201 biotype 2 on the basis of physiological and biochemical characteristics and the results of 16S rRNA gene and gyrB sequencing, virulence gene detection, and recursive infection experiments. To gain a comprehensive understanding of the pathogenicity and drug resistance of this strain, whole-genome sequencing was performed. Whole-genome analysis showed that the gene map of this strain was complete. The Virulence Factor Database annotation results showed that this strain had the key virulence factor genes vvhA and rtxA, which cause host disease. In addition, this strain had genes conferring resistance against cephalosporins, aminoglycosides, tetracyclines, and sulfonamides. Antimicrobial susceptibility testing confirmed the presence of these resistance genes identified in the genome. The results of this study show that V. vulnificus EPL 0201 biotype 2 is a multi-drug resistant strain with high pathogenicity. Vibrio vulnificus Pearl gentian grouper Virulence genes Antimicrobial susceptibility Whole-genome sequencing Microbiology Yating Wu verfasserin aut Haofeng Gao verfasserin aut Xuexin He verfasserin aut Qiang Yao verfasserin aut Zhanglei Yang verfasserin aut Jinyi Zhou verfasserin aut Linting Ji verfasserin aut Jinwei Gao verfasserin aut Xuying Jia verfasserin aut Yong Dou verfasserin aut Xiaoyu Wang verfasserin aut Peng Shao verfasserin aut In BMC Microbiology BMC, 2003 22(2022), 1, Seite 15 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:22 year:2022 number:1 pages:15 https://doi.org/10.1186/s12866-022-02610-1 kostenfrei https://doaj.org/article/479482bb142a4155ba2dba90002ae00f kostenfrei https://doi.org/10.1186/s12866-022-02610-1 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2022 1 15
spelling	10.1186/s12866-022-02610-1 doi (DE-627)DOAJ028154525 (DE-599)DOAJ479482bb142a4155ba2dba90002ae00f DE-627 ger DE-627 rakwb eng QR1-502 Zun Wu verfasserin aut Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Vibrio vulnificus is a pathogenic bacterium that causes disease in marine fish, affecting fish farming and human health worldwide. In May 2021, in the Bohai Bay region, a disease broke out in commercially farmed pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus), causing huge economic losses. The diseased fish had skin lesions, water accumulation in their abdomens, and showed tissue and organ damage. V. vulnificus biotype 2 has been reported in eels and other marine fish, but it is less reported in pearl gentian grouper. In this study, the pathogenic strain isolated from diseased fish was identified as V. vulnificus EPL 0201 biotype 2 on the basis of physiological and biochemical characteristics and the results of 16S rRNA gene and gyrB sequencing, virulence gene detection, and recursive infection experiments. To gain a comprehensive understanding of the pathogenicity and drug resistance of this strain, whole-genome sequencing was performed. Whole-genome analysis showed that the gene map of this strain was complete. The Virulence Factor Database annotation results showed that this strain had the key virulence factor genes vvhA and rtxA, which cause host disease. In addition, this strain had genes conferring resistance against cephalosporins, aminoglycosides, tetracyclines, and sulfonamides. Antimicrobial susceptibility testing confirmed the presence of these resistance genes identified in the genome. The results of this study show that V. vulnificus EPL 0201 biotype 2 is a multi-drug resistant strain with high pathogenicity. Vibrio vulnificus Pearl gentian grouper Virulence genes Antimicrobial susceptibility Whole-genome sequencing Microbiology Yating Wu verfasserin aut Haofeng Gao verfasserin aut Xuexin He verfasserin aut Qiang Yao verfasserin aut Zhanglei Yang verfasserin aut Jinyi Zhou verfasserin aut Linting Ji verfasserin aut Jinwei Gao verfasserin aut Xuying Jia verfasserin aut Yong Dou verfasserin aut Xiaoyu Wang verfasserin aut Peng Shao verfasserin aut In BMC Microbiology BMC, 2003 22(2022), 1, Seite 15 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:22 year:2022 number:1 pages:15 https://doi.org/10.1186/s12866-022-02610-1 kostenfrei https://doaj.org/article/479482bb142a4155ba2dba90002ae00f kostenfrei https://doi.org/10.1186/s12866-022-02610-1 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2022 1 15
allfields_unstemmed	10.1186/s12866-022-02610-1 doi (DE-627)DOAJ028154525 (DE-599)DOAJ479482bb142a4155ba2dba90002ae00f DE-627 ger DE-627 rakwb eng QR1-502 Zun Wu verfasserin aut Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Vibrio vulnificus is a pathogenic bacterium that causes disease in marine fish, affecting fish farming and human health worldwide. In May 2021, in the Bohai Bay region, a disease broke out in commercially farmed pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus), causing huge economic losses. The diseased fish had skin lesions, water accumulation in their abdomens, and showed tissue and organ damage. V. vulnificus biotype 2 has been reported in eels and other marine fish, but it is less reported in pearl gentian grouper. In this study, the pathogenic strain isolated from diseased fish was identified as V. vulnificus EPL 0201 biotype 2 on the basis of physiological and biochemical characteristics and the results of 16S rRNA gene and gyrB sequencing, virulence gene detection, and recursive infection experiments. To gain a comprehensive understanding of the pathogenicity and drug resistance of this strain, whole-genome sequencing was performed. Whole-genome analysis showed that the gene map of this strain was complete. The Virulence Factor Database annotation results showed that this strain had the key virulence factor genes vvhA and rtxA, which cause host disease. In addition, this strain had genes conferring resistance against cephalosporins, aminoglycosides, tetracyclines, and sulfonamides. Antimicrobial susceptibility testing confirmed the presence of these resistance genes identified in the genome. The results of this study show that V. vulnificus EPL 0201 biotype 2 is a multi-drug resistant strain with high pathogenicity. Vibrio vulnificus Pearl gentian grouper Virulence genes Antimicrobial susceptibility Whole-genome sequencing Microbiology Yating Wu verfasserin aut Haofeng Gao verfasserin aut Xuexin He verfasserin aut Qiang Yao verfasserin aut Zhanglei Yang verfasserin aut Jinyi Zhou verfasserin aut Linting Ji verfasserin aut Jinwei Gao verfasserin aut Xuying Jia verfasserin aut Yong Dou verfasserin aut Xiaoyu Wang verfasserin aut Peng Shao verfasserin aut In BMC Microbiology BMC, 2003 22(2022), 1, Seite 15 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:22 year:2022 number:1 pages:15 https://doi.org/10.1186/s12866-022-02610-1 kostenfrei https://doaj.org/article/479482bb142a4155ba2dba90002ae00f kostenfrei https://doi.org/10.1186/s12866-022-02610-1 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2022 1 15
allfieldsGer	10.1186/s12866-022-02610-1 doi (DE-627)DOAJ028154525 (DE-599)DOAJ479482bb142a4155ba2dba90002ae00f DE-627 ger DE-627 rakwb eng QR1-502 Zun Wu verfasserin aut Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Vibrio vulnificus is a pathogenic bacterium that causes disease in marine fish, affecting fish farming and human health worldwide. In May 2021, in the Bohai Bay region, a disease broke out in commercially farmed pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus), causing huge economic losses. The diseased fish had skin lesions, water accumulation in their abdomens, and showed tissue and organ damage. V. vulnificus biotype 2 has been reported in eels and other marine fish, but it is less reported in pearl gentian grouper. In this study, the pathogenic strain isolated from diseased fish was identified as V. vulnificus EPL 0201 biotype 2 on the basis of physiological and biochemical characteristics and the results of 16S rRNA gene and gyrB sequencing, virulence gene detection, and recursive infection experiments. To gain a comprehensive understanding of the pathogenicity and drug resistance of this strain, whole-genome sequencing was performed. Whole-genome analysis showed that the gene map of this strain was complete. The Virulence Factor Database annotation results showed that this strain had the key virulence factor genes vvhA and rtxA, which cause host disease. In addition, this strain had genes conferring resistance against cephalosporins, aminoglycosides, tetracyclines, and sulfonamides. Antimicrobial susceptibility testing confirmed the presence of these resistance genes identified in the genome. The results of this study show that V. vulnificus EPL 0201 biotype 2 is a multi-drug resistant strain with high pathogenicity. Vibrio vulnificus Pearl gentian grouper Virulence genes Antimicrobial susceptibility Whole-genome sequencing Microbiology Yating Wu verfasserin aut Haofeng Gao verfasserin aut Xuexin He verfasserin aut Qiang Yao verfasserin aut Zhanglei Yang verfasserin aut Jinyi Zhou verfasserin aut Linting Ji verfasserin aut Jinwei Gao verfasserin aut Xuying Jia verfasserin aut Yong Dou verfasserin aut Xiaoyu Wang verfasserin aut Peng Shao verfasserin aut In BMC Microbiology BMC, 2003 22(2022), 1, Seite 15 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:22 year:2022 number:1 pages:15 https://doi.org/10.1186/s12866-022-02610-1 kostenfrei https://doaj.org/article/479482bb142a4155ba2dba90002ae00f kostenfrei https://doi.org/10.1186/s12866-022-02610-1 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2022 1 15
allfieldsSound	10.1186/s12866-022-02610-1 doi (DE-627)DOAJ028154525 (DE-599)DOAJ479482bb142a4155ba2dba90002ae00f DE-627 ger DE-627 rakwb eng QR1-502 Zun Wu verfasserin aut Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Vibrio vulnificus is a pathogenic bacterium that causes disease in marine fish, affecting fish farming and human health worldwide. In May 2021, in the Bohai Bay region, a disease broke out in commercially farmed pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus), causing huge economic losses. The diseased fish had skin lesions, water accumulation in their abdomens, and showed tissue and organ damage. V. vulnificus biotype 2 has been reported in eels and other marine fish, but it is less reported in pearl gentian grouper. In this study, the pathogenic strain isolated from diseased fish was identified as V. vulnificus EPL 0201 biotype 2 on the basis of physiological and biochemical characteristics and the results of 16S rRNA gene and gyrB sequencing, virulence gene detection, and recursive infection experiments. To gain a comprehensive understanding of the pathogenicity and drug resistance of this strain, whole-genome sequencing was performed. Whole-genome analysis showed that the gene map of this strain was complete. The Virulence Factor Database annotation results showed that this strain had the key virulence factor genes vvhA and rtxA, which cause host disease. In addition, this strain had genes conferring resistance against cephalosporins, aminoglycosides, tetracyclines, and sulfonamides. Antimicrobial susceptibility testing confirmed the presence of these resistance genes identified in the genome. The results of this study show that V. vulnificus EPL 0201 biotype 2 is a multi-drug resistant strain with high pathogenicity. Vibrio vulnificus Pearl gentian grouper Virulence genes Antimicrobial susceptibility Whole-genome sequencing Microbiology Yating Wu verfasserin aut Haofeng Gao verfasserin aut Xuexin He verfasserin aut Qiang Yao verfasserin aut Zhanglei Yang verfasserin aut Jinyi Zhou verfasserin aut Linting Ji verfasserin aut Jinwei Gao verfasserin aut Xuying Jia verfasserin aut Yong Dou verfasserin aut Xiaoyu Wang verfasserin aut Peng Shao verfasserin aut In BMC Microbiology BMC, 2003 22(2022), 1, Seite 15 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:22 year:2022 number:1 pages:15 https://doi.org/10.1186/s12866-022-02610-1 kostenfrei https://doaj.org/article/479482bb142a4155ba2dba90002ae00f kostenfrei https://doi.org/10.1186/s12866-022-02610-1 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2022 1 15
language	English
source	In BMC Microbiology 22(2022), 1, Seite 15 volume:22 year:2022 number:1 pages:15
sourceStr	In BMC Microbiology 22(2022), 1, Seite 15 volume:22 year:2022 number:1 pages:15
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Vibrio vulnificus Pearl gentian grouper Virulence genes Antimicrobial susceptibility Whole-genome sequencing Microbiology
isfreeaccess_bool	true
container_title	BMC Microbiology
authorswithroles_txt_mv	Zun Wu @@aut@@ Yating Wu @@aut@@ Haofeng Gao @@aut@@ Xuexin He @@aut@@ Qiang Yao @@aut@@ Zhanglei Yang @@aut@@ Jinyi Zhou @@aut@@ Linting Ji @@aut@@ Jinwei Gao @@aut@@ Xuying Jia @@aut@@ Yong Dou @@aut@@ Xiaoyu Wang @@aut@@ Peng Shao @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	326644997
id	DOAJ028154525
language_de	englisch
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In May 2021, in the Bohai Bay region, a disease broke out in commercially farmed pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus), causing huge economic losses. The diseased fish had skin lesions, water accumulation in their abdomens, and showed tissue and organ damage. V. vulnificus biotype 2 has been reported in eels and other marine fish, but it is less reported in pearl gentian grouper. In this study, the pathogenic strain isolated from diseased fish was identified as V. vulnificus EPL 0201 biotype 2 on the basis of physiological and biochemical characteristics and the results of 16S rRNA gene and gyrB sequencing, virulence gene detection, and recursive infection experiments. To gain a comprehensive understanding of the pathogenicity and drug resistance of this strain, whole-genome sequencing was performed. Whole-genome analysis showed that the gene map of this strain was complete. 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callnumber-first	Q - Science
author	Zun Wu
spellingShingle	Zun Wu misc QR1-502 misc Vibrio vulnificus misc Pearl gentian grouper misc Virulence genes misc Antimicrobial susceptibility misc Whole-genome sequencing misc Microbiology Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China
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topic_title	QR1-502 Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China Vibrio vulnificus Pearl gentian grouper Virulence genes Antimicrobial susceptibility Whole-genome sequencing
topic	misc QR1-502 misc Vibrio vulnificus misc Pearl gentian grouper misc Virulence genes misc Antimicrobial susceptibility misc Whole-genome sequencing misc Microbiology
topic_unstemmed	misc QR1-502 misc Vibrio vulnificus misc Pearl gentian grouper misc Virulence genes misc Antimicrobial susceptibility misc Whole-genome sequencing misc Microbiology
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title	Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China
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title_full	Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China
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title_sort	identification and whole-genome sequencing analysis of vibrio vulnificus strains causing pearl gentian grouper disease in china
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title_auth	Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China
abstract	Abstract Vibrio vulnificus is a pathogenic bacterium that causes disease in marine fish, affecting fish farming and human health worldwide. In May 2021, in the Bohai Bay region, a disease broke out in commercially farmed pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus), causing huge economic losses. The diseased fish had skin lesions, water accumulation in their abdomens, and showed tissue and organ damage. V. vulnificus biotype 2 has been reported in eels and other marine fish, but it is less reported in pearl gentian grouper. In this study, the pathogenic strain isolated from diseased fish was identified as V. vulnificus EPL 0201 biotype 2 on the basis of physiological and biochemical characteristics and the results of 16S rRNA gene and gyrB sequencing, virulence gene detection, and recursive infection experiments. To gain a comprehensive understanding of the pathogenicity and drug resistance of this strain, whole-genome sequencing was performed. Whole-genome analysis showed that the gene map of this strain was complete. The Virulence Factor Database annotation results showed that this strain had the key virulence factor genes vvhA and rtxA, which cause host disease. In addition, this strain had genes conferring resistance against cephalosporins, aminoglycosides, tetracyclines, and sulfonamides. Antimicrobial susceptibility testing confirmed the presence of these resistance genes identified in the genome. The results of this study show that V. vulnificus EPL 0201 biotype 2 is a multi-drug resistant strain with high pathogenicity.
abstractGer	Abstract Vibrio vulnificus is a pathogenic bacterium that causes disease in marine fish, affecting fish farming and human health worldwide. In May 2021, in the Bohai Bay region, a disease broke out in commercially farmed pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus), causing huge economic losses. The diseased fish had skin lesions, water accumulation in their abdomens, and showed tissue and organ damage. V. vulnificus biotype 2 has been reported in eels and other marine fish, but it is less reported in pearl gentian grouper. In this study, the pathogenic strain isolated from diseased fish was identified as V. vulnificus EPL 0201 biotype 2 on the basis of physiological and biochemical characteristics and the results of 16S rRNA gene and gyrB sequencing, virulence gene detection, and recursive infection experiments. To gain a comprehensive understanding of the pathogenicity and drug resistance of this strain, whole-genome sequencing was performed. Whole-genome analysis showed that the gene map of this strain was complete. The Virulence Factor Database annotation results showed that this strain had the key virulence factor genes vvhA and rtxA, which cause host disease. In addition, this strain had genes conferring resistance against cephalosporins, aminoglycosides, tetracyclines, and sulfonamides. Antimicrobial susceptibility testing confirmed the presence of these resistance genes identified in the genome. The results of this study show that V. vulnificus EPL 0201 biotype 2 is a multi-drug resistant strain with high pathogenicity.
abstract_unstemmed	Abstract Vibrio vulnificus is a pathogenic bacterium that causes disease in marine fish, affecting fish farming and human health worldwide. In May 2021, in the Bohai Bay region, a disease broke out in commercially farmed pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus), causing huge economic losses. The diseased fish had skin lesions, water accumulation in their abdomens, and showed tissue and organ damage. V. vulnificus biotype 2 has been reported in eels and other marine fish, but it is less reported in pearl gentian grouper. In this study, the pathogenic strain isolated from diseased fish was identified as V. vulnificus EPL 0201 biotype 2 on the basis of physiological and biochemical characteristics and the results of 16S rRNA gene and gyrB sequencing, virulence gene detection, and recursive infection experiments. To gain a comprehensive understanding of the pathogenicity and drug resistance of this strain, whole-genome sequencing was performed. Whole-genome analysis showed that the gene map of this strain was complete. The Virulence Factor Database annotation results showed that this strain had the key virulence factor genes vvhA and rtxA, which cause host disease. In addition, this strain had genes conferring resistance against cephalosporins, aminoglycosides, tetracyclines, and sulfonamides. Antimicrobial susceptibility testing confirmed the presence of these resistance genes identified in the genome. The results of this study show that V. vulnificus EPL 0201 biotype 2 is a multi-drug resistant strain with high pathogenicity.
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title_short	Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China
url	https://doi.org/10.1186/s12866-022-02610-1 https://doaj.org/article/479482bb142a4155ba2dba90002ae00f https://doaj.org/toc/1471-2180
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Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China

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