Protocol to photoactivate adipose-derived stem cell differentiation using a tightly-focused femtosecond laser

Summary: The technology to induce stem cell differentiation is of great importance in life science, stem cell therapy, and regenerative medicine. Here, we detail steps to noninvasively activate stem cell differentiation in vitro and in vivo using a tightly focused femtosecond laser. We describe how...
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Gespeichert in:

Autor*in:	Wanyi Tang [verfasserIn] Haipeng Wang [verfasserIn] Hao He [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Microscopy Neuroscience Stem Cells Cell Differentiation Biotechnology and bioengineering

Übergeordnetes Werk:	In: STAR Protocols - Elsevier, 2020, 3(2022), 3, Seite 101574-
Übergeordnetes Werk:	volume:3 ; year:2022 ; number:3 ; pages:101574-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.xpro.2022.101574

Katalog-ID:	DOAJ028620917

Internformat


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allfields	10.1016/j.xpro.2022.101574 doi (DE-627)DOAJ028620917 (DE-599)DOAJ27aa29c19aa54fac814e7ba88fe90f68 DE-627 ger DE-627 rakwb eng Q1-390 Wanyi Tang verfasserin aut Protocol to photoactivate adipose-derived stem cell differentiation using a tightly-focused femtosecond laser 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: The technology to induce stem cell differentiation is of great importance in life science, stem cell therapy, and regenerative medicine. Here, we detail steps to noninvasively activate stem cell differentiation in vitro and in vivo using a tightly focused femtosecond laser. We describe how a single-time transient photoactivation can initiate differentiation without any gene engineering, exogenous substances, or physical contact. This protocol enables the differentiation of adipose-derived stem cells to osteoblasts in vitro and cerebellar granule neuron progenitors to granule neurons in vivo.For complete details on the use and execution of this protocol, please refer to Tang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Microscopy Neuroscience Stem Cells Cell Differentiation Biotechnology and bioengineering Science (General) Haipeng Wang verfasserin aut Hao He verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 3, Seite 101574- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:3 pages:101574- https://doi.org/10.1016/j.xpro.2022.101574 kostenfrei https://doaj.org/article/27aa29c19aa54fac814e7ba88fe90f68 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166722004543 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 3 101574-
spelling	10.1016/j.xpro.2022.101574 doi (DE-627)DOAJ028620917 (DE-599)DOAJ27aa29c19aa54fac814e7ba88fe90f68 DE-627 ger DE-627 rakwb eng Q1-390 Wanyi Tang verfasserin aut Protocol to photoactivate adipose-derived stem cell differentiation using a tightly-focused femtosecond laser 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: The technology to induce stem cell differentiation is of great importance in life science, stem cell therapy, and regenerative medicine. Here, we detail steps to noninvasively activate stem cell differentiation in vitro and in vivo using a tightly focused femtosecond laser. We describe how a single-time transient photoactivation can initiate differentiation without any gene engineering, exogenous substances, or physical contact. This protocol enables the differentiation of adipose-derived stem cells to osteoblasts in vitro and cerebellar granule neuron progenitors to granule neurons in vivo.For complete details on the use and execution of this protocol, please refer to Tang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Microscopy Neuroscience Stem Cells Cell Differentiation Biotechnology and bioengineering Science (General) Haipeng Wang verfasserin aut Hao He verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 3, Seite 101574- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:3 pages:101574- https://doi.org/10.1016/j.xpro.2022.101574 kostenfrei https://doaj.org/article/27aa29c19aa54fac814e7ba88fe90f68 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166722004543 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 3 101574-
allfields_unstemmed	10.1016/j.xpro.2022.101574 doi (DE-627)DOAJ028620917 (DE-599)DOAJ27aa29c19aa54fac814e7ba88fe90f68 DE-627 ger DE-627 rakwb eng Q1-390 Wanyi Tang verfasserin aut Protocol to photoactivate adipose-derived stem cell differentiation using a tightly-focused femtosecond laser 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: The technology to induce stem cell differentiation is of great importance in life science, stem cell therapy, and regenerative medicine. Here, we detail steps to noninvasively activate stem cell differentiation in vitro and in vivo using a tightly focused femtosecond laser. We describe how a single-time transient photoactivation can initiate differentiation without any gene engineering, exogenous substances, or physical contact. This protocol enables the differentiation of adipose-derived stem cells to osteoblasts in vitro and cerebellar granule neuron progenitors to granule neurons in vivo.For complete details on the use and execution of this protocol, please refer to Tang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Microscopy Neuroscience Stem Cells Cell Differentiation Biotechnology and bioengineering Science (General) Haipeng Wang verfasserin aut Hao He verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 3, Seite 101574- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:3 pages:101574- https://doi.org/10.1016/j.xpro.2022.101574 kostenfrei https://doaj.org/article/27aa29c19aa54fac814e7ba88fe90f68 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166722004543 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 3 101574-
allfieldsGer	10.1016/j.xpro.2022.101574 doi (DE-627)DOAJ028620917 (DE-599)DOAJ27aa29c19aa54fac814e7ba88fe90f68 DE-627 ger DE-627 rakwb eng Q1-390 Wanyi Tang verfasserin aut Protocol to photoactivate adipose-derived stem cell differentiation using a tightly-focused femtosecond laser 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: The technology to induce stem cell differentiation is of great importance in life science, stem cell therapy, and regenerative medicine. Here, we detail steps to noninvasively activate stem cell differentiation in vitro and in vivo using a tightly focused femtosecond laser. We describe how a single-time transient photoactivation can initiate differentiation without any gene engineering, exogenous substances, or physical contact. This protocol enables the differentiation of adipose-derived stem cells to osteoblasts in vitro and cerebellar granule neuron progenitors to granule neurons in vivo.For complete details on the use and execution of this protocol, please refer to Tang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Microscopy Neuroscience Stem Cells Cell Differentiation Biotechnology and bioengineering Science (General) Haipeng Wang verfasserin aut Hao He verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 3, Seite 101574- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:3 pages:101574- https://doi.org/10.1016/j.xpro.2022.101574 kostenfrei https://doaj.org/article/27aa29c19aa54fac814e7ba88fe90f68 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166722004543 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 3 101574-
allfieldsSound	10.1016/j.xpro.2022.101574 doi (DE-627)DOAJ028620917 (DE-599)DOAJ27aa29c19aa54fac814e7ba88fe90f68 DE-627 ger DE-627 rakwb eng Q1-390 Wanyi Tang verfasserin aut Protocol to photoactivate adipose-derived stem cell differentiation using a tightly-focused femtosecond laser 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: The technology to induce stem cell differentiation is of great importance in life science, stem cell therapy, and regenerative medicine. Here, we detail steps to noninvasively activate stem cell differentiation in vitro and in vivo using a tightly focused femtosecond laser. We describe how a single-time transient photoactivation can initiate differentiation without any gene engineering, exogenous substances, or physical contact. This protocol enables the differentiation of adipose-derived stem cells to osteoblasts in vitro and cerebellar granule neuron progenitors to granule neurons in vivo.For complete details on the use and execution of this protocol, please refer to Tang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Microscopy Neuroscience Stem Cells Cell Differentiation Biotechnology and bioengineering Science (General) Haipeng Wang verfasserin aut Hao He verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 3, Seite 101574- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:3 pages:101574- https://doi.org/10.1016/j.xpro.2022.101574 kostenfrei https://doaj.org/article/27aa29c19aa54fac814e7ba88fe90f68 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166722004543 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 3 101574-
language	English
source	In STAR Protocols 3(2022), 3, Seite 101574- volume:3 year:2022 number:3 pages:101574-
sourceStr	In STAR Protocols 3(2022), 3, Seite 101574- volume:3 year:2022 number:3 pages:101574-
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Microscopy Neuroscience Stem Cells Cell Differentiation Biotechnology and bioengineering Science (General)
isfreeaccess_bool	true
container_title	STAR Protocols
authorswithroles_txt_mv	Wanyi Tang @@aut@@ Haipeng Wang @@aut@@ Hao He @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	1747970557
id	DOAJ028620917
language_de	englisch
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callnumber-first	Q - Science
author	Wanyi Tang
spellingShingle	Wanyi Tang misc Q1-390 misc Microscopy misc Neuroscience misc Stem Cells misc Cell Differentiation misc Biotechnology and bioengineering misc Science (General) Protocol to photoactivate adipose-derived stem cell differentiation using a tightly-focused femtosecond laser
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topic_title	Q1-390 Protocol to photoactivate adipose-derived stem cell differentiation using a tightly-focused femtosecond laser Microscopy Neuroscience Stem Cells Cell Differentiation Biotechnology and bioengineering
topic	misc Q1-390 misc Microscopy misc Neuroscience misc Stem Cells misc Cell Differentiation misc Biotechnology and bioengineering misc Science (General)
topic_unstemmed	misc Q1-390 misc Microscopy misc Neuroscience misc Stem Cells misc Cell Differentiation misc Biotechnology and bioengineering misc Science (General)
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title	Protocol to photoactivate adipose-derived stem cell differentiation using a tightly-focused femtosecond laser
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title_full	Protocol to photoactivate adipose-derived stem cell differentiation using a tightly-focused femtosecond laser
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title_sort	protocol to photoactivate adipose-derived stem cell differentiation using a tightly-focused femtosecond laser
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title_auth	Protocol to photoactivate adipose-derived stem cell differentiation using a tightly-focused femtosecond laser
abstract	Summary: The technology to induce stem cell differentiation is of great importance in life science, stem cell therapy, and regenerative medicine. Here, we detail steps to noninvasively activate stem cell differentiation in vitro and in vivo using a tightly focused femtosecond laser. We describe how a single-time transient photoactivation can initiate differentiation without any gene engineering, exogenous substances, or physical contact. This protocol enables the differentiation of adipose-derived stem cells to osteoblasts in vitro and cerebellar granule neuron progenitors to granule neurons in vivo.For complete details on the use and execution of this protocol, please refer to Tang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
abstractGer	Summary: The technology to induce stem cell differentiation is of great importance in life science, stem cell therapy, and regenerative medicine. Here, we detail steps to noninvasively activate stem cell differentiation in vitro and in vivo using a tightly focused femtosecond laser. We describe how a single-time transient photoactivation can initiate differentiation without any gene engineering, exogenous substances, or physical contact. This protocol enables the differentiation of adipose-derived stem cells to osteoblasts in vitro and cerebellar granule neuron progenitors to granule neurons in vivo.For complete details on the use and execution of this protocol, please refer to Tang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
abstract_unstemmed	Summary: The technology to induce stem cell differentiation is of great importance in life science, stem cell therapy, and regenerative medicine. Here, we detail steps to noninvasively activate stem cell differentiation in vitro and in vivo using a tightly focused femtosecond laser. We describe how a single-time transient photoactivation can initiate differentiation without any gene engineering, exogenous substances, or physical contact. This protocol enables the differentiation of adipose-derived stem cells to osteoblasts in vitro and cerebellar granule neuron progenitors to granule neurons in vivo.For complete details on the use and execution of this protocol, please refer to Tang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
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title_short	Protocol to photoactivate adipose-derived stem cell differentiation using a tightly-focused femtosecond laser
url	https://doi.org/10.1016/j.xpro.2022.101574 https://doaj.org/article/27aa29c19aa54fac814e7ba88fe90f68 http://www.sciencedirect.com/science/article/pii/S2666166722004543 https://doaj.org/toc/2666-1667
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up_date	2024-07-03T18:34:01.771Z
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Protocol to photoactivate adipose-derived stem cell differentiation using a tightly-focused femtosecond laser

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