Clinically relevant orthotopic xenograft models of patient-derived glioblastoma in zebrafish

An accurate prediction of the intracranial infiltration tendency and drug response of individual glioblastoma (GBM) cells is essential for personalized prognosis and treatment for this disease. However, the clinical utility of mouse patient-derived orthotopic xenograft (PDOX) models remains limited...
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Autor*in:	Xiaolin Ai [verfasserIn] Zengpanpan Ye [verfasserIn] Chaoxin Xiao [verfasserIn] Jian Zhong [verfasserIn] Joseph J. Lancman [verfasserIn] Xuelan Chen [verfasserIn] Xiangyu Pan [verfasserIn] Yu Yang [verfasserIn] Lin Zhou [verfasserIn] Xiang Wang [verfasserIn] Huashan Shi [verfasserIn] Dongmei Zhang [verfasserIn] Yuqin Yao [verfasserIn] Dan Cao [verfasserIn] Chengjian Zhao [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	zebrafish glioblastoma heterogeneity blood–brain barrier pdx pdox

Übergeordnetes Werk:	In: Disease Models & Mechanisms - The Company of Biologists, 2011, 15(2022), 4
Übergeordnetes Werk:	volume:15 ; year:2022 ; number:4

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1242/dmm.049109

Katalog-ID:	DOAJ028890485

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callnumber-first	R - Medicine
author	Xiaolin Ai
spellingShingle	Xiaolin Ai misc RB1-214 misc zebrafish misc glioblastoma misc heterogeneity misc blood–brain barrier misc pdx misc pdox misc Medicine misc R misc Pathology Clinically relevant orthotopic xenograft models of patient-derived glioblastoma in zebrafish
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topic_title	RB1-214 Clinically relevant orthotopic xenograft models of patient-derived glioblastoma in zebrafish zebrafish glioblastoma heterogeneity blood–brain barrier pdx pdox
topic	misc RB1-214 misc zebrafish misc glioblastoma misc heterogeneity misc blood–brain barrier misc pdx misc pdox misc Medicine misc R misc Pathology
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title	Clinically relevant orthotopic xenograft models of patient-derived glioblastoma in zebrafish
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title_full	Clinically relevant orthotopic xenograft models of patient-derived glioblastoma in zebrafish
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author_browse	Xiaolin Ai Zengpanpan Ye Chaoxin Xiao Jian Zhong Joseph J. Lancman Xuelan Chen Xiangyu Pan Yu Yang Lin Zhou Xiang Wang Huashan Shi Dongmei Zhang Yuqin Yao Dan Cao Chengjian Zhao
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title_sort	clinically relevant orthotopic xenograft models of patient-derived glioblastoma in zebrafish
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title_auth	Clinically relevant orthotopic xenograft models of patient-derived glioblastoma in zebrafish
abstract	An accurate prediction of the intracranial infiltration tendency and drug response of individual glioblastoma (GBM) cells is essential for personalized prognosis and treatment for this disease. However, the clinical utility of mouse patient-derived orthotopic xenograft (PDOX) models remains limited given current technical constraints, including difficulty in generating sufficient sample numbers from small tissue samples and a long latency period for results. To overcome these issues, we established zebrafish GBM xenografts of diverse origin, which can tolerate intracranial engraftment and maintain their unique histological features. Subsequent single-cell RNA-sequencing (scRNA-seq) analysis confirmed significant transcriptional identity to that of invading GBM microtumors observed in the proportionally larger brains of model animals and humans. Endothelial scRNA-seq confirmed that the zebrafish blood–brain barrier is homologous to the mammalian blood–brain barrier. Finally, we established a rapid and efficient zebrafish PDOX (zPDOX) model, which can predict long-term outcomes of GBM patients within 20 days. The zPDOX model provides a novel avenue for precision medicine of GBM, especially for the evaluation of intracranial infiltration tendency and prediction of individual drug sensitivity.
abstractGer	An accurate prediction of the intracranial infiltration tendency and drug response of individual glioblastoma (GBM) cells is essential for personalized prognosis and treatment for this disease. However, the clinical utility of mouse patient-derived orthotopic xenograft (PDOX) models remains limited given current technical constraints, including difficulty in generating sufficient sample numbers from small tissue samples and a long latency period for results. To overcome these issues, we established zebrafish GBM xenografts of diverse origin, which can tolerate intracranial engraftment and maintain their unique histological features. Subsequent single-cell RNA-sequencing (scRNA-seq) analysis confirmed significant transcriptional identity to that of invading GBM microtumors observed in the proportionally larger brains of model animals and humans. Endothelial scRNA-seq confirmed that the zebrafish blood–brain barrier is homologous to the mammalian blood–brain barrier. Finally, we established a rapid and efficient zebrafish PDOX (zPDOX) model, which can predict long-term outcomes of GBM patients within 20 days. The zPDOX model provides a novel avenue for precision medicine of GBM, especially for the evaluation of intracranial infiltration tendency and prediction of individual drug sensitivity.
abstract_unstemmed	An accurate prediction of the intracranial infiltration tendency and drug response of individual glioblastoma (GBM) cells is essential for personalized prognosis and treatment for this disease. However, the clinical utility of mouse patient-derived orthotopic xenograft (PDOX) models remains limited given current technical constraints, including difficulty in generating sufficient sample numbers from small tissue samples and a long latency period for results. To overcome these issues, we established zebrafish GBM xenografts of diverse origin, which can tolerate intracranial engraftment and maintain their unique histological features. Subsequent single-cell RNA-sequencing (scRNA-seq) analysis confirmed significant transcriptional identity to that of invading GBM microtumors observed in the proportionally larger brains of model animals and humans. Endothelial scRNA-seq confirmed that the zebrafish blood–brain barrier is homologous to the mammalian blood–brain barrier. Finally, we established a rapid and efficient zebrafish PDOX (zPDOX) model, which can predict long-term outcomes of GBM patients within 20 days. The zPDOX model provides a novel avenue for precision medicine of GBM, especially for the evaluation of intracranial infiltration tendency and prediction of individual drug sensitivity.
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title_short	Clinically relevant orthotopic xenograft models of patient-derived glioblastoma in zebrafish
url	https://doi.org/10.1242/dmm.049109 https://doaj.org/article/61dea9b1204249fe97e11002a8215fb4 http://dmm.biologists.org/content/15/4/dmm049109 https://doaj.org/toc/1754-8403 https://doaj.org/toc/1754-8411
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author2	Zengpanpan Ye Chaoxin Xiao Jian Zhong Joseph J. Lancman Xuelan Chen Xiangyu Pan Yu Yang Lin Zhou Xiang Wang Huashan Shi Dongmei Zhang Yuqin Yao Dan Cao Chengjian Zhao
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doi_str	10.1242/dmm.049109
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up_date	2024-07-03T19:58:46.791Z
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Clinically relevant orthotopic xenograft models of patient-derived glioblastoma in zebrafish

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