Factors Affecting the Expression of Recombinant Protein and Improvement Strategies in Chinese Hamster Ovary Cells

Recombinant therapeutic proteins (RTPs) are important parts of biopharmaceuticals. Chinese hamster ovary cells (CHO) have become the main cell hosts for the production of most RTPs approved for marketing because of their high-density suspension growth characteristics, and similar human post-translat...
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Autor*in:	Zheng-Mei Li [verfasserIn] Zhen-Lin Fan [verfasserIn] Xiao-Yin Wang [verfasserIn] Tian-Yun Wang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	recombinant protein CHO difficult to express expression vector recombinant therapeutic proteins

Übergeordnetes Werk:	In: Frontiers in Bioengineering and Biotechnology - Frontiers Media S.A., 2014, 10(2022)
Übergeordnetes Werk:	volume:10 ; year:2022

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fbioe.2022.880155

Katalog-ID:	DOAJ028966163

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520			\|a Recombinant therapeutic proteins (RTPs) are important parts of biopharmaceuticals. Chinese hamster ovary cells (CHO) have become the main cell hosts for the production of most RTPs approved for marketing because of their high-density suspension growth characteristics, and similar human post-translational modification patterns et al. In recent years, many studies have been performed on CHO cell expression systems, and the yields and quality of recombinant protein expression have been greatly improved. However, the expression levels of some proteins are still low or even difficult-to express in CHO cells. It is urgent further to increase the yields and to express successfully the “difficult-to express” protein in CHO cells. The process of recombinant protein expression of is a complex, involving multiple steps such as transcription, translation, folding processing and secretion. In addition, the inherent characteristics of molecular will also affect the production of protein. Here, we reviewed the factors affecting the expression of recombinant protein and improvement strategies in CHO cells.
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allfields	10.3389/fbioe.2022.880155 doi (DE-627)DOAJ028966163 (DE-599)DOAJ8a0c7067aee64c41a4451ed40fe44318 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Zheng-Mei Li verfasserin aut Factors Affecting the Expression of Recombinant Protein and Improvement Strategies in Chinese Hamster Ovary Cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Recombinant therapeutic proteins (RTPs) are important parts of biopharmaceuticals. Chinese hamster ovary cells (CHO) have become the main cell hosts for the production of most RTPs approved for marketing because of their high-density suspension growth characteristics, and similar human post-translational modification patterns et al. In recent years, many studies have been performed on CHO cell expression systems, and the yields and quality of recombinant protein expression have been greatly improved. However, the expression levels of some proteins are still low or even difficult-to express in CHO cells. It is urgent further to increase the yields and to express successfully the “difficult-to express” protein in CHO cells. The process of recombinant protein expression of is a complex, involving multiple steps such as transcription, translation, folding processing and secretion. In addition, the inherent characteristics of molecular will also affect the production of protein. Here, we reviewed the factors affecting the expression of recombinant protein and improvement strategies in CHO cells. recombinant protein CHO difficult to express expression vector recombinant therapeutic proteins Biotechnology Zheng-Mei Li verfasserin aut Zhen-Lin Fan verfasserin aut Zhen-Lin Fan verfasserin aut Xiao-Yin Wang verfasserin aut Xiao-Yin Wang verfasserin aut Tian-Yun Wang verfasserin aut Tian-Yun Wang verfasserin aut In Frontiers in Bioengineering and Biotechnology Frontiers Media S.A., 2014 10(2022) (DE-627)74950403X (DE-600)2719493-0 22964185 nnns volume:10 year:2022 https://doi.org/10.3389/fbioe.2022.880155 kostenfrei https://doaj.org/article/8a0c7067aee64c41a4451ed40fe44318 kostenfrei https://www.frontiersin.org/articles/10.3389/fbioe.2022.880155/full kostenfrei https://doaj.org/toc/2296-4185 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2022
spelling	10.3389/fbioe.2022.880155 doi (DE-627)DOAJ028966163 (DE-599)DOAJ8a0c7067aee64c41a4451ed40fe44318 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Zheng-Mei Li verfasserin aut Factors Affecting the Expression of Recombinant Protein and Improvement Strategies in Chinese Hamster Ovary Cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Recombinant therapeutic proteins (RTPs) are important parts of biopharmaceuticals. Chinese hamster ovary cells (CHO) have become the main cell hosts for the production of most RTPs approved for marketing because of their high-density suspension growth characteristics, and similar human post-translational modification patterns et al. In recent years, many studies have been performed on CHO cell expression systems, and the yields and quality of recombinant protein expression have been greatly improved. However, the expression levels of some proteins are still low or even difficult-to express in CHO cells. It is urgent further to increase the yields and to express successfully the “difficult-to express” protein in CHO cells. The process of recombinant protein expression of is a complex, involving multiple steps such as transcription, translation, folding processing and secretion. In addition, the inherent characteristics of molecular will also affect the production of protein. Here, we reviewed the factors affecting the expression of recombinant protein and improvement strategies in CHO cells. recombinant protein CHO difficult to express expression vector recombinant therapeutic proteins Biotechnology Zheng-Mei Li verfasserin aut Zhen-Lin Fan verfasserin aut Zhen-Lin Fan verfasserin aut Xiao-Yin Wang verfasserin aut Xiao-Yin Wang verfasserin aut Tian-Yun Wang verfasserin aut Tian-Yun Wang verfasserin aut In Frontiers in Bioengineering and Biotechnology Frontiers Media S.A., 2014 10(2022) (DE-627)74950403X (DE-600)2719493-0 22964185 nnns volume:10 year:2022 https://doi.org/10.3389/fbioe.2022.880155 kostenfrei https://doaj.org/article/8a0c7067aee64c41a4451ed40fe44318 kostenfrei https://www.frontiersin.org/articles/10.3389/fbioe.2022.880155/full kostenfrei https://doaj.org/toc/2296-4185 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2022
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allfieldsSound	10.3389/fbioe.2022.880155 doi (DE-627)DOAJ028966163 (DE-599)DOAJ8a0c7067aee64c41a4451ed40fe44318 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Zheng-Mei Li verfasserin aut Factors Affecting the Expression of Recombinant Protein and Improvement Strategies in Chinese Hamster Ovary Cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Recombinant therapeutic proteins (RTPs) are important parts of biopharmaceuticals. Chinese hamster ovary cells (CHO) have become the main cell hosts for the production of most RTPs approved for marketing because of their high-density suspension growth characteristics, and similar human post-translational modification patterns et al. In recent years, many studies have been performed on CHO cell expression systems, and the yields and quality of recombinant protein expression have been greatly improved. However, the expression levels of some proteins are still low or even difficult-to express in CHO cells. It is urgent further to increase the yields and to express successfully the “difficult-to express” protein in CHO cells. The process of recombinant protein expression of is a complex, involving multiple steps such as transcription, translation, folding processing and secretion. In addition, the inherent characteristics of molecular will also affect the production of protein. Here, we reviewed the factors affecting the expression of recombinant protein and improvement strategies in CHO cells. recombinant protein CHO difficult to express expression vector recombinant therapeutic proteins Biotechnology Zheng-Mei Li verfasserin aut Zhen-Lin Fan verfasserin aut Zhen-Lin Fan verfasserin aut Xiao-Yin Wang verfasserin aut Xiao-Yin Wang verfasserin aut Tian-Yun Wang verfasserin aut Tian-Yun Wang verfasserin aut In Frontiers in Bioengineering and Biotechnology Frontiers Media S.A., 2014 10(2022) (DE-627)74950403X (DE-600)2719493-0 22964185 nnns volume:10 year:2022 https://doi.org/10.3389/fbioe.2022.880155 kostenfrei https://doaj.org/article/8a0c7067aee64c41a4451ed40fe44318 kostenfrei https://www.frontiersin.org/articles/10.3389/fbioe.2022.880155/full kostenfrei https://doaj.org/toc/2296-4185 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2022
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source	In Frontiers in Bioengineering and Biotechnology 10(2022) volume:10 year:2022
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topic_facet	recombinant protein CHO difficult to express expression vector recombinant therapeutic proteins Biotechnology
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container_title	Frontiers in Bioengineering and Biotechnology
authorswithroles_txt_mv	Zheng-Mei Li @@aut@@ Zhen-Lin Fan @@aut@@ Xiao-Yin Wang @@aut@@ Tian-Yun Wang @@aut@@
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callnumber-first	T - Technology
author	Zheng-Mei Li
spellingShingle	Zheng-Mei Li misc TP248.13-248.65 misc recombinant protein misc CHO misc difficult to express misc expression vector misc recombinant therapeutic proteins misc Biotechnology Factors Affecting the Expression of Recombinant Protein and Improvement Strategies in Chinese Hamster Ovary Cells
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topic_title	TP248.13-248.65 Factors Affecting the Expression of Recombinant Protein and Improvement Strategies in Chinese Hamster Ovary Cells recombinant protein CHO difficult to express expression vector recombinant therapeutic proteins
topic	misc TP248.13-248.65 misc recombinant protein misc CHO misc difficult to express misc expression vector misc recombinant therapeutic proteins misc Biotechnology
topic_unstemmed	misc TP248.13-248.65 misc recombinant protein misc CHO misc difficult to express misc expression vector misc recombinant therapeutic proteins misc Biotechnology
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title	Factors Affecting the Expression of Recombinant Protein and Improvement Strategies in Chinese Hamster Ovary Cells
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title_full	Factors Affecting the Expression of Recombinant Protein and Improvement Strategies in Chinese Hamster Ovary Cells
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title_sort	factors affecting the expression of recombinant protein and improvement strategies in chinese hamster ovary cells
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title_auth	Factors Affecting the Expression of Recombinant Protein and Improvement Strategies in Chinese Hamster Ovary Cells
abstract	Recombinant therapeutic proteins (RTPs) are important parts of biopharmaceuticals. Chinese hamster ovary cells (CHO) have become the main cell hosts for the production of most RTPs approved for marketing because of their high-density suspension growth characteristics, and similar human post-translational modification patterns et al. In recent years, many studies have been performed on CHO cell expression systems, and the yields and quality of recombinant protein expression have been greatly improved. However, the expression levels of some proteins are still low or even difficult-to express in CHO cells. It is urgent further to increase the yields and to express successfully the “difficult-to express” protein in CHO cells. The process of recombinant protein expression of is a complex, involving multiple steps such as transcription, translation, folding processing and secretion. In addition, the inherent characteristics of molecular will also affect the production of protein. Here, we reviewed the factors affecting the expression of recombinant protein and improvement strategies in CHO cells.
abstractGer	Recombinant therapeutic proteins (RTPs) are important parts of biopharmaceuticals. Chinese hamster ovary cells (CHO) have become the main cell hosts for the production of most RTPs approved for marketing because of their high-density suspension growth characteristics, and similar human post-translational modification patterns et al. In recent years, many studies have been performed on CHO cell expression systems, and the yields and quality of recombinant protein expression have been greatly improved. However, the expression levels of some proteins are still low or even difficult-to express in CHO cells. It is urgent further to increase the yields and to express successfully the “difficult-to express” protein in CHO cells. The process of recombinant protein expression of is a complex, involving multiple steps such as transcription, translation, folding processing and secretion. In addition, the inherent characteristics of molecular will also affect the production of protein. Here, we reviewed the factors affecting the expression of recombinant protein and improvement strategies in CHO cells.
abstract_unstemmed	Recombinant therapeutic proteins (RTPs) are important parts of biopharmaceuticals. Chinese hamster ovary cells (CHO) have become the main cell hosts for the production of most RTPs approved for marketing because of their high-density suspension growth characteristics, and similar human post-translational modification patterns et al. In recent years, many studies have been performed on CHO cell expression systems, and the yields and quality of recombinant protein expression have been greatly improved. However, the expression levels of some proteins are still low or even difficult-to express in CHO cells. It is urgent further to increase the yields and to express successfully the “difficult-to express” protein in CHO cells. The process of recombinant protein expression of is a complex, involving multiple steps such as transcription, translation, folding processing and secretion. In addition, the inherent characteristics of molecular will also affect the production of protein. Here, we reviewed the factors affecting the expression of recombinant protein and improvement strategies in CHO cells.
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title_short	Factors Affecting the Expression of Recombinant Protein and Improvement Strategies in Chinese Hamster Ovary Cells
url	https://doi.org/10.3389/fbioe.2022.880155 https://doaj.org/article/8a0c7067aee64c41a4451ed40fe44318 https://www.frontiersin.org/articles/10.3389/fbioe.2022.880155/full https://doaj.org/toc/2296-4185
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