Test system creation for analysis of template switching effect during a reverse transcription <i<in vitro</i<
We investigated an in vitro system for the analysis of template switching effect of RNA-dependent DNA-polymerase during reverse transcription reaction. An RT-PCR-based approach was realized. An RNA sample was used as a template. The RNA contains a region with a stable secondary structure flanked by...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
A. Y. Komkov [verfasserIn] Y. B. Lebedev [verfasserIn] V. I. Shvets [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Russisch |
| Erschienen: |
2011 |
|---|
| Schlagwörter: |
|---|
| Übergeordnetes Werk: |
In: Тонкие химические технологии - MIREA - Russian Technological University, 2019, 6(2011), 6, Seite 77-83 |
|---|---|
| Übergeordnetes Werk: |
volume:6 ; year:2011 ; number:6 ; pages:77-83 |
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Komkov</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Test system creation for analysis of template switching effect during a reverse transcription <i<in vitro</i<</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2011</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">We investigated an in vitro system for the analysis of template switching effect of RNA-dependent DNA-polymerase during reverse transcription reaction. An RT-PCR-based approach was realized. An RNA sample was used as a template. The RNA contains a region with a stable secondary structure flanked by two homologous sequences. The described system can find use for testing overall performance of new and existing forms of the reverse transcriptase.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">alu repeat</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">retroelement</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">reverse transcriptase</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">template switching effect</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Chemistry</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Y. B. Lebedev</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">V. I. Shvets</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Тонкие химические технологии</subfield><subfield code="d">MIREA - Russian Technological University, 2019</subfield><subfield code="g">6(2011), 6, Seite 77-83</subfield><subfield code="w">(DE-627)175513021X</subfield><subfield code="x">26867575</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:6</subfield><subfield code="g">year:2011</subfield><subfield code="g">number:6</subfield><subfield code="g">pages:77-83</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/fc2c41bbbf9f47b6a193e92c6e34613b</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.finechem-mirea.ru/jour/article/view/1191</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2410-6593</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2686-7575</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">6</subfield><subfield code="j">2011</subfield><subfield code="e">6</subfield><subfield code="h">77-83</subfield></datafield></record></collection>
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