Therapeutic effects of oral administration of lytic Salmonella phages in a mouse model of non-typhoidal salmonellosis

Acute non-typhoidal salmonellosis (NTS) caused by a Gram-negative bacterium Salmonella enterica serovar Typhimurium (S. Tm) is one of the most common bacterial foodborne diseases worldwide. Bacteriophages (phages) can specifically target and lyse their host bacteria, including the multidrug-resistan...
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Autor*in:	Chutikarn Sukjoi [verfasserIn] Songphon Buddhasiri [verfasserIn] Arishabhas Tantibhadrasapa [verfasserIn] Thattawan Kaewsakhorn [verfasserIn] Preeda Phothaworn [verfasserIn] Janet Y. Nale [verfasserIn] Angela V. Lopez-Garcia [verfasserIn] Manal AbuOun [verfasserIn] Muna F. Anjum [verfasserIn] Danish J. Malik [verfasserIn] Edouard E. Galyov [verfasserIn] Martha R. J. Clokie [verfasserIn] Sunee Korbsrisate [verfasserIn] Parameth Thiennimitr [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	bacteriophage therapy Salmonella enterica Typhimurium foodborne pathogen acute non-typhoidal salmonellosis mouse colitis model inflammatory response

Übergeordnetes Werk:	In: Frontiers in Microbiology - Frontiers Media S.A., 2011, 13(2022)
Übergeordnetes Werk:	volume:13 ; year:2022

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fmicb.2022.955136

Katalog-ID:	DOAJ029483042

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allfields	10.3389/fmicb.2022.955136 doi (DE-627)DOAJ029483042 (DE-599)DOAJ660bedd5af484cc2afc38d54c4d71966 DE-627 ger DE-627 rakwb eng QR1-502 Chutikarn Sukjoi verfasserin aut Therapeutic effects of oral administration of lytic Salmonella phages in a mouse model of non-typhoidal salmonellosis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Acute non-typhoidal salmonellosis (NTS) caused by a Gram-negative bacterium Salmonella enterica serovar Typhimurium (S. Tm) is one of the most common bacterial foodborne diseases worldwide. Bacteriophages (phages) can specifically target and lyse their host bacteria, including the multidrug-resistant strains, without collateral damage to other bacteria in the community. However, the therapeutic use of Salmonella phages in vivo is still poorly investigated. Salmonella phages ST-W77 and SE-W109 have previously been shown by our group to be useful for biocontrol properties. Here, we tested whether phages ST-W77 and SE-W109 can reduce Salmonella invasion into cultured human cells and confer a therapeutic benefit for acute NTS in a mammalian host. Human colonocytes, T84 cells, were treated with phages ST-W77, SE-W109, and its combination for 5 min before S. Tm infection. Gentamicin protection assays demonstrated that ST-W77 and SE-W109 significantly reduced S. Tm invasion and inflammatory response in human colonocytes. Next, streptomycin-pretreated mice were orally infected with S. Tm (108 CFU/mouse) and treated with a single or a combination of ST-W77 and SE-W109 (1010 PFU/mouse for 4 days) by oral feeding. Our data showed that phage-treated mice had lower S. Tm numbers and tissue inflammation compared to the untreated mice. Our study also revealed that ST-W77 and SE-W109 persist in the mouse gut lumen, but not in systemic sites. Together, these data suggested that Salmonella phages ST-W77 and SE-W109 could be further developed as an alternative approach for treating an acute NTS in mammalian hosts. bacteriophage therapy Salmonella enterica Typhimurium foodborne pathogen acute non-typhoidal salmonellosis mouse colitis model inflammatory response Microbiology Songphon Buddhasiri verfasserin aut Arishabhas Tantibhadrasapa verfasserin aut Thattawan Kaewsakhorn verfasserin aut Preeda Phothaworn verfasserin aut Janet Y. Nale verfasserin aut Angela V. Lopez-Garcia verfasserin aut Manal AbuOun verfasserin aut Muna F. Anjum verfasserin aut Danish J. Malik verfasserin aut Edouard E. Galyov verfasserin aut Martha R. J. Clokie verfasserin aut Sunee Korbsrisate verfasserin aut Parameth Thiennimitr verfasserin aut Parameth Thiennimitr verfasserin aut Parameth Thiennimitr verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 13(2022) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:13 year:2022 https://doi.org/10.3389/fmicb.2022.955136 kostenfrei https://doaj.org/article/660bedd5af484cc2afc38d54c4d71966 kostenfrei https://www.frontiersin.org/articles/10.3389/fmicb.2022.955136/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022
spelling	10.3389/fmicb.2022.955136 doi (DE-627)DOAJ029483042 (DE-599)DOAJ660bedd5af484cc2afc38d54c4d71966 DE-627 ger DE-627 rakwb eng QR1-502 Chutikarn Sukjoi verfasserin aut Therapeutic effects of oral administration of lytic Salmonella phages in a mouse model of non-typhoidal salmonellosis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Acute non-typhoidal salmonellosis (NTS) caused by a Gram-negative bacterium Salmonella enterica serovar Typhimurium (S. Tm) is one of the most common bacterial foodborne diseases worldwide. Bacteriophages (phages) can specifically target and lyse their host bacteria, including the multidrug-resistant strains, without collateral damage to other bacteria in the community. However, the therapeutic use of Salmonella phages in vivo is still poorly investigated. Salmonella phages ST-W77 and SE-W109 have previously been shown by our group to be useful for biocontrol properties. Here, we tested whether phages ST-W77 and SE-W109 can reduce Salmonella invasion into cultured human cells and confer a therapeutic benefit for acute NTS in a mammalian host. Human colonocytes, T84 cells, were treated with phages ST-W77, SE-W109, and its combination for 5 min before S. Tm infection. Gentamicin protection assays demonstrated that ST-W77 and SE-W109 significantly reduced S. Tm invasion and inflammatory response in human colonocytes. Next, streptomycin-pretreated mice were orally infected with S. Tm (108 CFU/mouse) and treated with a single or a combination of ST-W77 and SE-W109 (1010 PFU/mouse for 4 days) by oral feeding. Our data showed that phage-treated mice had lower S. Tm numbers and tissue inflammation compared to the untreated mice. Our study also revealed that ST-W77 and SE-W109 persist in the mouse gut lumen, but not in systemic sites. Together, these data suggested that Salmonella phages ST-W77 and SE-W109 could be further developed as an alternative approach for treating an acute NTS in mammalian hosts. bacteriophage therapy Salmonella enterica Typhimurium foodborne pathogen acute non-typhoidal salmonellosis mouse colitis model inflammatory response Microbiology Songphon Buddhasiri verfasserin aut Arishabhas Tantibhadrasapa verfasserin aut Thattawan Kaewsakhorn verfasserin aut Preeda Phothaworn verfasserin aut Janet Y. Nale verfasserin aut Angela V. Lopez-Garcia verfasserin aut Manal AbuOun verfasserin aut Muna F. Anjum verfasserin aut Danish J. Malik verfasserin aut Edouard E. Galyov verfasserin aut Martha R. J. Clokie verfasserin aut Sunee Korbsrisate verfasserin aut Parameth Thiennimitr verfasserin aut Parameth Thiennimitr verfasserin aut Parameth Thiennimitr verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 13(2022) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:13 year:2022 https://doi.org/10.3389/fmicb.2022.955136 kostenfrei https://doaj.org/article/660bedd5af484cc2afc38d54c4d71966 kostenfrei https://www.frontiersin.org/articles/10.3389/fmicb.2022.955136/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022
allfields_unstemmed	10.3389/fmicb.2022.955136 doi (DE-627)DOAJ029483042 (DE-599)DOAJ660bedd5af484cc2afc38d54c4d71966 DE-627 ger DE-627 rakwb eng QR1-502 Chutikarn Sukjoi verfasserin aut Therapeutic effects of oral administration of lytic Salmonella phages in a mouse model of non-typhoidal salmonellosis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Acute non-typhoidal salmonellosis (NTS) caused by a Gram-negative bacterium Salmonella enterica serovar Typhimurium (S. Tm) is one of the most common bacterial foodborne diseases worldwide. Bacteriophages (phages) can specifically target and lyse their host bacteria, including the multidrug-resistant strains, without collateral damage to other bacteria in the community. However, the therapeutic use of Salmonella phages in vivo is still poorly investigated. Salmonella phages ST-W77 and SE-W109 have previously been shown by our group to be useful for biocontrol properties. Here, we tested whether phages ST-W77 and SE-W109 can reduce Salmonella invasion into cultured human cells and confer a therapeutic benefit for acute NTS in a mammalian host. Human colonocytes, T84 cells, were treated with phages ST-W77, SE-W109, and its combination for 5 min before S. Tm infection. Gentamicin protection assays demonstrated that ST-W77 and SE-W109 significantly reduced S. Tm invasion and inflammatory response in human colonocytes. Next, streptomycin-pretreated mice were orally infected with S. Tm (108 CFU/mouse) and treated with a single or a combination of ST-W77 and SE-W109 (1010 PFU/mouse for 4 days) by oral feeding. Our data showed that phage-treated mice had lower S. Tm numbers and tissue inflammation compared to the untreated mice. Our study also revealed that ST-W77 and SE-W109 persist in the mouse gut lumen, but not in systemic sites. Together, these data suggested that Salmonella phages ST-W77 and SE-W109 could be further developed as an alternative approach for treating an acute NTS in mammalian hosts. bacteriophage therapy Salmonella enterica Typhimurium foodborne pathogen acute non-typhoidal salmonellosis mouse colitis model inflammatory response Microbiology Songphon Buddhasiri verfasserin aut Arishabhas Tantibhadrasapa verfasserin aut Thattawan Kaewsakhorn verfasserin aut Preeda Phothaworn verfasserin aut Janet Y. Nale verfasserin aut Angela V. Lopez-Garcia verfasserin aut Manal AbuOun verfasserin aut Muna F. Anjum verfasserin aut Danish J. Malik verfasserin aut Edouard E. Galyov verfasserin aut Martha R. J. Clokie verfasserin aut Sunee Korbsrisate verfasserin aut Parameth Thiennimitr verfasserin aut Parameth Thiennimitr verfasserin aut Parameth Thiennimitr verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 13(2022) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:13 year:2022 https://doi.org/10.3389/fmicb.2022.955136 kostenfrei https://doaj.org/article/660bedd5af484cc2afc38d54c4d71966 kostenfrei https://www.frontiersin.org/articles/10.3389/fmicb.2022.955136/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022
allfieldsGer	10.3389/fmicb.2022.955136 doi (DE-627)DOAJ029483042 (DE-599)DOAJ660bedd5af484cc2afc38d54c4d71966 DE-627 ger DE-627 rakwb eng QR1-502 Chutikarn Sukjoi verfasserin aut Therapeutic effects of oral administration of lytic Salmonella phages in a mouse model of non-typhoidal salmonellosis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Acute non-typhoidal salmonellosis (NTS) caused by a Gram-negative bacterium Salmonella enterica serovar Typhimurium (S. Tm) is one of the most common bacterial foodborne diseases worldwide. Bacteriophages (phages) can specifically target and lyse their host bacteria, including the multidrug-resistant strains, without collateral damage to other bacteria in the community. However, the therapeutic use of Salmonella phages in vivo is still poorly investigated. Salmonella phages ST-W77 and SE-W109 have previously been shown by our group to be useful for biocontrol properties. Here, we tested whether phages ST-W77 and SE-W109 can reduce Salmonella invasion into cultured human cells and confer a therapeutic benefit for acute NTS in a mammalian host. Human colonocytes, T84 cells, were treated with phages ST-W77, SE-W109, and its combination for 5 min before S. Tm infection. Gentamicin protection assays demonstrated that ST-W77 and SE-W109 significantly reduced S. Tm invasion and inflammatory response in human colonocytes. Next, streptomycin-pretreated mice were orally infected with S. Tm (108 CFU/mouse) and treated with a single or a combination of ST-W77 and SE-W109 (1010 PFU/mouse for 4 days) by oral feeding. Our data showed that phage-treated mice had lower S. Tm numbers and tissue inflammation compared to the untreated mice. Our study also revealed that ST-W77 and SE-W109 persist in the mouse gut lumen, but not in systemic sites. Together, these data suggested that Salmonella phages ST-W77 and SE-W109 could be further developed as an alternative approach for treating an acute NTS in mammalian hosts. bacteriophage therapy Salmonella enterica Typhimurium foodborne pathogen acute non-typhoidal salmonellosis mouse colitis model inflammatory response Microbiology Songphon Buddhasiri verfasserin aut Arishabhas Tantibhadrasapa verfasserin aut Thattawan Kaewsakhorn verfasserin aut Preeda Phothaworn verfasserin aut Janet Y. Nale verfasserin aut Angela V. Lopez-Garcia verfasserin aut Manal AbuOun verfasserin aut Muna F. Anjum verfasserin aut Danish J. Malik verfasserin aut Edouard E. Galyov verfasserin aut Martha R. J. Clokie verfasserin aut Sunee Korbsrisate verfasserin aut Parameth Thiennimitr verfasserin aut Parameth Thiennimitr verfasserin aut Parameth Thiennimitr verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 13(2022) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:13 year:2022 https://doi.org/10.3389/fmicb.2022.955136 kostenfrei https://doaj.org/article/660bedd5af484cc2afc38d54c4d71966 kostenfrei https://www.frontiersin.org/articles/10.3389/fmicb.2022.955136/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022
allfieldsSound	10.3389/fmicb.2022.955136 doi (DE-627)DOAJ029483042 (DE-599)DOAJ660bedd5af484cc2afc38d54c4d71966 DE-627 ger DE-627 rakwb eng QR1-502 Chutikarn Sukjoi verfasserin aut Therapeutic effects of oral administration of lytic Salmonella phages in a mouse model of non-typhoidal salmonellosis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Acute non-typhoidal salmonellosis (NTS) caused by a Gram-negative bacterium Salmonella enterica serovar Typhimurium (S. Tm) is one of the most common bacterial foodborne diseases worldwide. Bacteriophages (phages) can specifically target and lyse their host bacteria, including the multidrug-resistant strains, without collateral damage to other bacteria in the community. However, the therapeutic use of Salmonella phages in vivo is still poorly investigated. Salmonella phages ST-W77 and SE-W109 have previously been shown by our group to be useful for biocontrol properties. Here, we tested whether phages ST-W77 and SE-W109 can reduce Salmonella invasion into cultured human cells and confer a therapeutic benefit for acute NTS in a mammalian host. Human colonocytes, T84 cells, were treated with phages ST-W77, SE-W109, and its combination for 5 min before S. Tm infection. Gentamicin protection assays demonstrated that ST-W77 and SE-W109 significantly reduced S. Tm invasion and inflammatory response in human colonocytes. Next, streptomycin-pretreated mice were orally infected with S. Tm (108 CFU/mouse) and treated with a single or a combination of ST-W77 and SE-W109 (1010 PFU/mouse for 4 days) by oral feeding. Our data showed that phage-treated mice had lower S. Tm numbers and tissue inflammation compared to the untreated mice. Our study also revealed that ST-W77 and SE-W109 persist in the mouse gut lumen, but not in systemic sites. Together, these data suggested that Salmonella phages ST-W77 and SE-W109 could be further developed as an alternative approach for treating an acute NTS in mammalian hosts. bacteriophage therapy Salmonella enterica Typhimurium foodborne pathogen acute non-typhoidal salmonellosis mouse colitis model inflammatory response Microbiology Songphon Buddhasiri verfasserin aut Arishabhas Tantibhadrasapa verfasserin aut Thattawan Kaewsakhorn verfasserin aut Preeda Phothaworn verfasserin aut Janet Y. Nale verfasserin aut Angela V. Lopez-Garcia verfasserin aut Manal AbuOun verfasserin aut Muna F. Anjum verfasserin aut Danish J. Malik verfasserin aut Edouard E. Galyov verfasserin aut Martha R. J. Clokie verfasserin aut Sunee Korbsrisate verfasserin aut Parameth Thiennimitr verfasserin aut Parameth Thiennimitr verfasserin aut Parameth Thiennimitr verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 13(2022) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:13 year:2022 https://doi.org/10.3389/fmicb.2022.955136 kostenfrei https://doaj.org/article/660bedd5af484cc2afc38d54c4d71966 kostenfrei https://www.frontiersin.org/articles/10.3389/fmicb.2022.955136/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022
language	English
source	In Frontiers in Microbiology 13(2022) volume:13 year:2022
sourceStr	In Frontiers in Microbiology 13(2022) volume:13 year:2022
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	bacteriophage therapy Salmonella enterica Typhimurium foodborne pathogen acute non-typhoidal salmonellosis mouse colitis model inflammatory response Microbiology
isfreeaccess_bool	true
container_title	Frontiers in Microbiology
authorswithroles_txt_mv	Chutikarn Sukjoi @@aut@@ Songphon Buddhasiri @@aut@@ Arishabhas Tantibhadrasapa @@aut@@ Thattawan Kaewsakhorn @@aut@@ Preeda Phothaworn @@aut@@ Janet Y. Nale @@aut@@ Angela V. Lopez-Garcia @@aut@@ Manal AbuOun @@aut@@ Muna F. Anjum @@aut@@ Danish J. Malik @@aut@@ Edouard E. Galyov @@aut@@ Martha R. J. Clokie @@aut@@ Sunee Korbsrisate @@aut@@ Parameth Thiennimitr @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	642889384
id	DOAJ029483042
language_de	englisch
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callnumber-first	Q - Science
author	Chutikarn Sukjoi
spellingShingle	Chutikarn Sukjoi misc QR1-502 misc bacteriophage therapy misc Salmonella enterica Typhimurium misc foodborne pathogen misc acute non-typhoidal salmonellosis misc mouse colitis model misc inflammatory response misc Microbiology Therapeutic effects of oral administration of lytic Salmonella phages in a mouse model of non-typhoidal salmonellosis
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topic_title	QR1-502 Therapeutic effects of oral administration of lytic Salmonella phages in a mouse model of non-typhoidal salmonellosis bacteriophage therapy Salmonella enterica Typhimurium foodborne pathogen acute non-typhoidal salmonellosis mouse colitis model inflammatory response
topic	misc QR1-502 misc bacteriophage therapy misc Salmonella enterica Typhimurium misc foodborne pathogen misc acute non-typhoidal salmonellosis misc mouse colitis model misc inflammatory response misc Microbiology
topic_unstemmed	misc QR1-502 misc bacteriophage therapy misc Salmonella enterica Typhimurium misc foodborne pathogen misc acute non-typhoidal salmonellosis misc mouse colitis model misc inflammatory response misc Microbiology
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title	Therapeutic effects of oral administration of lytic Salmonella phages in a mouse model of non-typhoidal salmonellosis
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title_full	Therapeutic effects of oral administration of lytic Salmonella phages in a mouse model of non-typhoidal salmonellosis
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author_browse	Chutikarn Sukjoi Songphon Buddhasiri Arishabhas Tantibhadrasapa Thattawan Kaewsakhorn Preeda Phothaworn Janet Y. Nale Angela V. Lopez-Garcia Manal AbuOun Muna F. Anjum Danish J. Malik Edouard E. Galyov Martha R. J. Clokie Sunee Korbsrisate Parameth Thiennimitr
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title_sort	therapeutic effects of oral administration of lytic salmonella phages in a mouse model of non-typhoidal salmonellosis
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title_auth	Therapeutic effects of oral administration of lytic Salmonella phages in a mouse model of non-typhoidal salmonellosis
abstract	Acute non-typhoidal salmonellosis (NTS) caused by a Gram-negative bacterium Salmonella enterica serovar Typhimurium (S. Tm) is one of the most common bacterial foodborne diseases worldwide. Bacteriophages (phages) can specifically target and lyse their host bacteria, including the multidrug-resistant strains, without collateral damage to other bacteria in the community. However, the therapeutic use of Salmonella phages in vivo is still poorly investigated. Salmonella phages ST-W77 and SE-W109 have previously been shown by our group to be useful for biocontrol properties. Here, we tested whether phages ST-W77 and SE-W109 can reduce Salmonella invasion into cultured human cells and confer a therapeutic benefit for acute NTS in a mammalian host. Human colonocytes, T84 cells, were treated with phages ST-W77, SE-W109, and its combination for 5 min before S. Tm infection. Gentamicin protection assays demonstrated that ST-W77 and SE-W109 significantly reduced S. Tm invasion and inflammatory response in human colonocytes. Next, streptomycin-pretreated mice were orally infected with S. Tm (108 CFU/mouse) and treated with a single or a combination of ST-W77 and SE-W109 (1010 PFU/mouse for 4 days) by oral feeding. Our data showed that phage-treated mice had lower S. Tm numbers and tissue inflammation compared to the untreated mice. Our study also revealed that ST-W77 and SE-W109 persist in the mouse gut lumen, but not in systemic sites. Together, these data suggested that Salmonella phages ST-W77 and SE-W109 could be further developed as an alternative approach for treating an acute NTS in mammalian hosts.
abstractGer	Acute non-typhoidal salmonellosis (NTS) caused by a Gram-negative bacterium Salmonella enterica serovar Typhimurium (S. Tm) is one of the most common bacterial foodborne diseases worldwide. Bacteriophages (phages) can specifically target and lyse their host bacteria, including the multidrug-resistant strains, without collateral damage to other bacteria in the community. However, the therapeutic use of Salmonella phages in vivo is still poorly investigated. Salmonella phages ST-W77 and SE-W109 have previously been shown by our group to be useful for biocontrol properties. Here, we tested whether phages ST-W77 and SE-W109 can reduce Salmonella invasion into cultured human cells and confer a therapeutic benefit for acute NTS in a mammalian host. Human colonocytes, T84 cells, were treated with phages ST-W77, SE-W109, and its combination for 5 min before S. Tm infection. Gentamicin protection assays demonstrated that ST-W77 and SE-W109 significantly reduced S. Tm invasion and inflammatory response in human colonocytes. Next, streptomycin-pretreated mice were orally infected with S. Tm (108 CFU/mouse) and treated with a single or a combination of ST-W77 and SE-W109 (1010 PFU/mouse for 4 days) by oral feeding. Our data showed that phage-treated mice had lower S. Tm numbers and tissue inflammation compared to the untreated mice. Our study also revealed that ST-W77 and SE-W109 persist in the mouse gut lumen, but not in systemic sites. Together, these data suggested that Salmonella phages ST-W77 and SE-W109 could be further developed as an alternative approach for treating an acute NTS in mammalian hosts.
abstract_unstemmed	Acute non-typhoidal salmonellosis (NTS) caused by a Gram-negative bacterium Salmonella enterica serovar Typhimurium (S. Tm) is one of the most common bacterial foodborne diseases worldwide. Bacteriophages (phages) can specifically target and lyse their host bacteria, including the multidrug-resistant strains, without collateral damage to other bacteria in the community. However, the therapeutic use of Salmonella phages in vivo is still poorly investigated. Salmonella phages ST-W77 and SE-W109 have previously been shown by our group to be useful for biocontrol properties. Here, we tested whether phages ST-W77 and SE-W109 can reduce Salmonella invasion into cultured human cells and confer a therapeutic benefit for acute NTS in a mammalian host. Human colonocytes, T84 cells, were treated with phages ST-W77, SE-W109, and its combination for 5 min before S. Tm infection. Gentamicin protection assays demonstrated that ST-W77 and SE-W109 significantly reduced S. Tm invasion and inflammatory response in human colonocytes. Next, streptomycin-pretreated mice were orally infected with S. Tm (108 CFU/mouse) and treated with a single or a combination of ST-W77 and SE-W109 (1010 PFU/mouse for 4 days) by oral feeding. Our data showed that phage-treated mice had lower S. Tm numbers and tissue inflammation compared to the untreated mice. Our study also revealed that ST-W77 and SE-W109 persist in the mouse gut lumen, but not in systemic sites. Together, these data suggested that Salmonella phages ST-W77 and SE-W109 could be further developed as an alternative approach for treating an acute NTS in mammalian hosts.
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title_short	Therapeutic effects of oral administration of lytic Salmonella phages in a mouse model of non-typhoidal salmonellosis
url	https://doi.org/10.3389/fmicb.2022.955136 https://doaj.org/article/660bedd5af484cc2afc38d54c4d71966 https://www.frontiersin.org/articles/10.3389/fmicb.2022.955136/full https://doaj.org/toc/1664-302X
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Therapeutic effects of oral administration of lytic Salmonella phages in a mouse model of non-typhoidal salmonellosis

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