A New Nano Adjuvant of PF3 Used for an Enhanced Hepatitis B Vaccine

Recombinant protein vaccines, with highly pure ingredients and good safety, are gradually replacing some attenuated and inactivated vaccines in clinical practice. However, since their low immunogenicity of the recombinant proteins, adjuvants are often needed to enhance immune response after vaccinat...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Pu Shan [verfasserIn] Zhibiao Wang [verfasserIn] Jilai Li [verfasserIn] Duoqian Wei [verfasserIn] Zhuan Zhang [verfasserIn] Shaojie Hao [verfasserIn] Yibo Hou [verfasserIn] Yunyang Wang [verfasserIn] Shuxiang Li [verfasserIn] Xudong Wang [verfasserIn] Jing Xu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	PF3 nano adjuvant ginsenoside Rg1 aluminum adjuvant hepatitis B surface antigen

Übergeordnetes Werk:	In: Frontiers in Bioengineering and Biotechnology - Frontiers Media S.A., 2014, 10(2022)
Übergeordnetes Werk:	volume:10 ; year:2022

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fbioe.2022.903424

Katalog-ID:	DOAJ029556201

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520			\|a Recombinant protein vaccines, with highly pure ingredients and good safety, are gradually replacing some attenuated and inactivated vaccines in clinical practice. However, since their low immunogenicity of the recombinant proteins, adjuvants are often needed to enhance immune response after vaccination. Aluminum adjuvant has been widely used in some vaccines for decades, it can induce strong humoral immunity, but the deficiency of cellular immunity limits its application for some vaccines. Therefore, it is urgently needed to develop novel adjuvant to increase not only humoral but also cellular immune response. To address this, we designed and prepared a new nano adjuvant (PF3) through microfluidization by the combination of saponin (Ginsenoside Rg1) and oil-in-water nano emulsion (NE) in the present study. As compared to aluminum adjuvant, PF3 had stronger humoral and cellular immune induction effect because of high cellular uptake and activization of immune response pathways. Furthermore, PF3 showed better immune enhancement and acceptable biosafety equivalent to that of aluminum adjuvant. In addition, no obvious changes of PF3 were observed in size and zeta potential after 12 weeks storage at 4 and 37°C, demonstrating its high stability in vitro. This study provided an adjuvant platform to replace traditional aluminum adjuvant in design of recombinant vaccines.
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allfields	10.3389/fbioe.2022.903424 doi (DE-627)DOAJ029556201 (DE-599)DOAJ0f83251805fe46899fe04091e9643573 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Pu Shan verfasserin aut A New Nano Adjuvant of PF3 Used for an Enhanced Hepatitis B Vaccine 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Recombinant protein vaccines, with highly pure ingredients and good safety, are gradually replacing some attenuated and inactivated vaccines in clinical practice. However, since their low immunogenicity of the recombinant proteins, adjuvants are often needed to enhance immune response after vaccination. Aluminum adjuvant has been widely used in some vaccines for decades, it can induce strong humoral immunity, but the deficiency of cellular immunity limits its application for some vaccines. Therefore, it is urgently needed to develop novel adjuvant to increase not only humoral but also cellular immune response. To address this, we designed and prepared a new nano adjuvant (PF3) through microfluidization by the combination of saponin (Ginsenoside Rg1) and oil-in-water nano emulsion (NE) in the present study. As compared to aluminum adjuvant, PF3 had stronger humoral and cellular immune induction effect because of high cellular uptake and activization of immune response pathways. Furthermore, PF3 showed better immune enhancement and acceptable biosafety equivalent to that of aluminum adjuvant. In addition, no obvious changes of PF3 were observed in size and zeta potential after 12 weeks storage at 4 and 37°C, demonstrating its high stability in vitro. This study provided an adjuvant platform to replace traditional aluminum adjuvant in design of recombinant vaccines. PF3 nano adjuvant ginsenoside Rg1 aluminum adjuvant hepatitis B surface antigen Biotechnology Zhibiao Wang verfasserin aut Jilai Li verfasserin aut Duoqian Wei verfasserin aut Zhuan Zhang verfasserin aut Shaojie Hao verfasserin aut Yibo Hou verfasserin aut Yunyang Wang verfasserin aut Shuxiang Li verfasserin aut Xudong Wang verfasserin aut Jing Xu verfasserin aut In Frontiers in Bioengineering and Biotechnology Frontiers Media S.A., 2014 10(2022) (DE-627)74950403X (DE-600)2719493-0 22964185 nnns volume:10 year:2022 https://doi.org/10.3389/fbioe.2022.903424 kostenfrei https://doaj.org/article/0f83251805fe46899fe04091e9643573 kostenfrei https://www.frontiersin.org/articles/10.3389/fbioe.2022.903424/full kostenfrei https://doaj.org/toc/2296-4185 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2022
spelling	10.3389/fbioe.2022.903424 doi (DE-627)DOAJ029556201 (DE-599)DOAJ0f83251805fe46899fe04091e9643573 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Pu Shan verfasserin aut A New Nano Adjuvant of PF3 Used for an Enhanced Hepatitis B Vaccine 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Recombinant protein vaccines, with highly pure ingredients and good safety, are gradually replacing some attenuated and inactivated vaccines in clinical practice. However, since their low immunogenicity of the recombinant proteins, adjuvants are often needed to enhance immune response after vaccination. Aluminum adjuvant has been widely used in some vaccines for decades, it can induce strong humoral immunity, but the deficiency of cellular immunity limits its application for some vaccines. Therefore, it is urgently needed to develop novel adjuvant to increase not only humoral but also cellular immune response. To address this, we designed and prepared a new nano adjuvant (PF3) through microfluidization by the combination of saponin (Ginsenoside Rg1) and oil-in-water nano emulsion (NE) in the present study. As compared to aluminum adjuvant, PF3 had stronger humoral and cellular immune induction effect because of high cellular uptake and activization of immune response pathways. Furthermore, PF3 showed better immune enhancement and acceptable biosafety equivalent to that of aluminum adjuvant. In addition, no obvious changes of PF3 were observed in size and zeta potential after 12 weeks storage at 4 and 37°C, demonstrating its high stability in vitro. This study provided an adjuvant platform to replace traditional aluminum adjuvant in design of recombinant vaccines. PF3 nano adjuvant ginsenoside Rg1 aluminum adjuvant hepatitis B surface antigen Biotechnology Zhibiao Wang verfasserin aut Jilai Li verfasserin aut Duoqian Wei verfasserin aut Zhuan Zhang verfasserin aut Shaojie Hao verfasserin aut Yibo Hou verfasserin aut Yunyang Wang verfasserin aut Shuxiang Li verfasserin aut Xudong Wang verfasserin aut Jing Xu verfasserin aut In Frontiers in Bioengineering and Biotechnology Frontiers Media S.A., 2014 10(2022) (DE-627)74950403X (DE-600)2719493-0 22964185 nnns volume:10 year:2022 https://doi.org/10.3389/fbioe.2022.903424 kostenfrei https://doaj.org/article/0f83251805fe46899fe04091e9643573 kostenfrei https://www.frontiersin.org/articles/10.3389/fbioe.2022.903424/full kostenfrei https://doaj.org/toc/2296-4185 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2022
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allfieldsGer	10.3389/fbioe.2022.903424 doi (DE-627)DOAJ029556201 (DE-599)DOAJ0f83251805fe46899fe04091e9643573 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Pu Shan verfasserin aut A New Nano Adjuvant of PF3 Used for an Enhanced Hepatitis B Vaccine 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Recombinant protein vaccines, with highly pure ingredients and good safety, are gradually replacing some attenuated and inactivated vaccines in clinical practice. However, since their low immunogenicity of the recombinant proteins, adjuvants are often needed to enhance immune response after vaccination. Aluminum adjuvant has been widely used in some vaccines for decades, it can induce strong humoral immunity, but the deficiency of cellular immunity limits its application for some vaccines. Therefore, it is urgently needed to develop novel adjuvant to increase not only humoral but also cellular immune response. To address this, we designed and prepared a new nano adjuvant (PF3) through microfluidization by the combination of saponin (Ginsenoside Rg1) and oil-in-water nano emulsion (NE) in the present study. As compared to aluminum adjuvant, PF3 had stronger humoral and cellular immune induction effect because of high cellular uptake and activization of immune response pathways. Furthermore, PF3 showed better immune enhancement and acceptable biosafety equivalent to that of aluminum adjuvant. In addition, no obvious changes of PF3 were observed in size and zeta potential after 12 weeks storage at 4 and 37°C, demonstrating its high stability in vitro. This study provided an adjuvant platform to replace traditional aluminum adjuvant in design of recombinant vaccines. PF3 nano adjuvant ginsenoside Rg1 aluminum adjuvant hepatitis B surface antigen Biotechnology Zhibiao Wang verfasserin aut Jilai Li verfasserin aut Duoqian Wei verfasserin aut Zhuan Zhang verfasserin aut Shaojie Hao verfasserin aut Yibo Hou verfasserin aut Yunyang Wang verfasserin aut Shuxiang Li verfasserin aut Xudong Wang verfasserin aut Jing Xu verfasserin aut In Frontiers in Bioengineering and Biotechnology Frontiers Media S.A., 2014 10(2022) (DE-627)74950403X (DE-600)2719493-0 22964185 nnns volume:10 year:2022 https://doi.org/10.3389/fbioe.2022.903424 kostenfrei https://doaj.org/article/0f83251805fe46899fe04091e9643573 kostenfrei https://www.frontiersin.org/articles/10.3389/fbioe.2022.903424/full kostenfrei https://doaj.org/toc/2296-4185 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2022
allfieldsSound	10.3389/fbioe.2022.903424 doi (DE-627)DOAJ029556201 (DE-599)DOAJ0f83251805fe46899fe04091e9643573 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Pu Shan verfasserin aut A New Nano Adjuvant of PF3 Used for an Enhanced Hepatitis B Vaccine 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Recombinant protein vaccines, with highly pure ingredients and good safety, are gradually replacing some attenuated and inactivated vaccines in clinical practice. However, since their low immunogenicity of the recombinant proteins, adjuvants are often needed to enhance immune response after vaccination. Aluminum adjuvant has been widely used in some vaccines for decades, it can induce strong humoral immunity, but the deficiency of cellular immunity limits its application for some vaccines. Therefore, it is urgently needed to develop novel adjuvant to increase not only humoral but also cellular immune response. To address this, we designed and prepared a new nano adjuvant (PF3) through microfluidization by the combination of saponin (Ginsenoside Rg1) and oil-in-water nano emulsion (NE) in the present study. As compared to aluminum adjuvant, PF3 had stronger humoral and cellular immune induction effect because of high cellular uptake and activization of immune response pathways. Furthermore, PF3 showed better immune enhancement and acceptable biosafety equivalent to that of aluminum adjuvant. In addition, no obvious changes of PF3 were observed in size and zeta potential after 12 weeks storage at 4 and 37°C, demonstrating its high stability in vitro. This study provided an adjuvant platform to replace traditional aluminum adjuvant in design of recombinant vaccines. PF3 nano adjuvant ginsenoside Rg1 aluminum adjuvant hepatitis B surface antigen Biotechnology Zhibiao Wang verfasserin aut Jilai Li verfasserin aut Duoqian Wei verfasserin aut Zhuan Zhang verfasserin aut Shaojie Hao verfasserin aut Yibo Hou verfasserin aut Yunyang Wang verfasserin aut Shuxiang Li verfasserin aut Xudong Wang verfasserin aut Jing Xu verfasserin aut In Frontiers in Bioengineering and Biotechnology Frontiers Media S.A., 2014 10(2022) (DE-627)74950403X (DE-600)2719493-0 22964185 nnns volume:10 year:2022 https://doi.org/10.3389/fbioe.2022.903424 kostenfrei https://doaj.org/article/0f83251805fe46899fe04091e9643573 kostenfrei https://www.frontiersin.org/articles/10.3389/fbioe.2022.903424/full kostenfrei https://doaj.org/toc/2296-4185 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2022
language	English
source	In Frontiers in Bioengineering and Biotechnology 10(2022) volume:10 year:2022
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topic_facet	PF3 nano adjuvant ginsenoside Rg1 aluminum adjuvant hepatitis B surface antigen Biotechnology
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container_title	Frontiers in Bioengineering and Biotechnology
authorswithroles_txt_mv	Pu Shan @@aut@@ Zhibiao Wang @@aut@@ Jilai Li @@aut@@ Duoqian Wei @@aut@@ Zhuan Zhang @@aut@@ Shaojie Hao @@aut@@ Yibo Hou @@aut@@ Yunyang Wang @@aut@@ Shuxiang Li @@aut@@ Xudong Wang @@aut@@ Jing Xu @@aut@@
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callnumber-first	T - Technology
author	Pu Shan
spellingShingle	Pu Shan misc TP248.13-248.65 misc PF3 misc nano adjuvant misc ginsenoside Rg1 misc aluminum adjuvant misc hepatitis B surface antigen misc Biotechnology A New Nano Adjuvant of PF3 Used for an Enhanced Hepatitis B Vaccine
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topic_title	TP248.13-248.65 A New Nano Adjuvant of PF3 Used for an Enhanced Hepatitis B Vaccine PF3 nano adjuvant ginsenoside Rg1 aluminum adjuvant hepatitis B surface antigen
topic	misc TP248.13-248.65 misc PF3 misc nano adjuvant misc ginsenoside Rg1 misc aluminum adjuvant misc hepatitis B surface antigen misc Biotechnology
topic_unstemmed	misc TP248.13-248.65 misc PF3 misc nano adjuvant misc ginsenoside Rg1 misc aluminum adjuvant misc hepatitis B surface antigen misc Biotechnology
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title	A New Nano Adjuvant of PF3 Used for an Enhanced Hepatitis B Vaccine
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title_full	A New Nano Adjuvant of PF3 Used for an Enhanced Hepatitis B Vaccine
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author_browse	Pu Shan Zhibiao Wang Jilai Li Duoqian Wei Zhuan Zhang Shaojie Hao Yibo Hou Yunyang Wang Shuxiang Li Xudong Wang Jing Xu
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title_sort	new nano adjuvant of pf3 used for an enhanced hepatitis b vaccine
callnumber	TP248.13-248.65
title_auth	A New Nano Adjuvant of PF3 Used for an Enhanced Hepatitis B Vaccine
abstract	Recombinant protein vaccines, with highly pure ingredients and good safety, are gradually replacing some attenuated and inactivated vaccines in clinical practice. However, since their low immunogenicity of the recombinant proteins, adjuvants are often needed to enhance immune response after vaccination. Aluminum adjuvant has been widely used in some vaccines for decades, it can induce strong humoral immunity, but the deficiency of cellular immunity limits its application for some vaccines. Therefore, it is urgently needed to develop novel adjuvant to increase not only humoral but also cellular immune response. To address this, we designed and prepared a new nano adjuvant (PF3) through microfluidization by the combination of saponin (Ginsenoside Rg1) and oil-in-water nano emulsion (NE) in the present study. As compared to aluminum adjuvant, PF3 had stronger humoral and cellular immune induction effect because of high cellular uptake and activization of immune response pathways. Furthermore, PF3 showed better immune enhancement and acceptable biosafety equivalent to that of aluminum adjuvant. In addition, no obvious changes of PF3 were observed in size and zeta potential after 12 weeks storage at 4 and 37°C, demonstrating its high stability in vitro. This study provided an adjuvant platform to replace traditional aluminum adjuvant in design of recombinant vaccines.
abstractGer	Recombinant protein vaccines, with highly pure ingredients and good safety, are gradually replacing some attenuated and inactivated vaccines in clinical practice. However, since their low immunogenicity of the recombinant proteins, adjuvants are often needed to enhance immune response after vaccination. Aluminum adjuvant has been widely used in some vaccines for decades, it can induce strong humoral immunity, but the deficiency of cellular immunity limits its application for some vaccines. Therefore, it is urgently needed to develop novel adjuvant to increase not only humoral but also cellular immune response. To address this, we designed and prepared a new nano adjuvant (PF3) through microfluidization by the combination of saponin (Ginsenoside Rg1) and oil-in-water nano emulsion (NE) in the present study. As compared to aluminum adjuvant, PF3 had stronger humoral and cellular immune induction effect because of high cellular uptake and activization of immune response pathways. Furthermore, PF3 showed better immune enhancement and acceptable biosafety equivalent to that of aluminum adjuvant. In addition, no obvious changes of PF3 were observed in size and zeta potential after 12 weeks storage at 4 and 37°C, demonstrating its high stability in vitro. This study provided an adjuvant platform to replace traditional aluminum adjuvant in design of recombinant vaccines.
abstract_unstemmed	Recombinant protein vaccines, with highly pure ingredients and good safety, are gradually replacing some attenuated and inactivated vaccines in clinical practice. However, since their low immunogenicity of the recombinant proteins, adjuvants are often needed to enhance immune response after vaccination. Aluminum adjuvant has been widely used in some vaccines for decades, it can induce strong humoral immunity, but the deficiency of cellular immunity limits its application for some vaccines. Therefore, it is urgently needed to develop novel adjuvant to increase not only humoral but also cellular immune response. To address this, we designed and prepared a new nano adjuvant (PF3) through microfluidization by the combination of saponin (Ginsenoside Rg1) and oil-in-water nano emulsion (NE) in the present study. As compared to aluminum adjuvant, PF3 had stronger humoral and cellular immune induction effect because of high cellular uptake and activization of immune response pathways. Furthermore, PF3 showed better immune enhancement and acceptable biosafety equivalent to that of aluminum adjuvant. In addition, no obvious changes of PF3 were observed in size and zeta potential after 12 weeks storage at 4 and 37°C, demonstrating its high stability in vitro. This study provided an adjuvant platform to replace traditional aluminum adjuvant in design of recombinant vaccines.
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title_short	A New Nano Adjuvant of PF3 Used for an Enhanced Hepatitis B Vaccine
url	https://doi.org/10.3389/fbioe.2022.903424 https://doaj.org/article/0f83251805fe46899fe04091e9643573 https://www.frontiersin.org/articles/10.3389/fbioe.2022.903424/full https://doaj.org/toc/2296-4185
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author2	Zhibiao Wang Jilai Li Duoqian Wei Zhuan Zhang Shaojie Hao Yibo Hou Yunyang Wang Shuxiang Li Xudong Wang Jing Xu
author2Str	Zhibiao Wang Jilai Li Duoqian Wei Zhuan Zhang Shaojie Hao Yibo Hou Yunyang Wang Shuxiang Li Xudong Wang Jing Xu
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doi_str	10.3389/fbioe.2022.903424
callnumber-a	TP248.13-248.65
up_date	2024-07-03T23:26:06.467Z
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A New Nano Adjuvant of PF3 Used for an Enhanced Hepatitis B Vaccine

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