miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit

Mammalian hair formation is critically determined by the growth of hair follicles (HF). MiRNAs are crucial in the periodic development of hair follicles; they maintain epidermal homeostasis by targeting genes and influencing the activity of signaling pathways and related regulators. Our study discov...
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Autor*in:	Fan Yao [verfasserIn] Bohao Zhao [verfasserIn] Shuaishuai Hu [verfasserIn] Shaocheng Bai [verfasserIn] Rongshuai Jin [verfasserIn] Chen Zhang [verfasserIn] Yang Chen [verfasserIn] Xinsheng Wu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	miR-129-5p DPCs proliferation and apoptosis

Übergeordnetes Werk:	In: Genes - MDPI AG, 2010, 13(2022), 4, p 679
Übergeordnetes Werk:	volume:13 ; year:2022 ; number:4, p 679

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/genes13040679

Katalog-ID:	DOAJ029887119

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520			\|a Mammalian hair formation is critically determined by the growth of hair follicles (HF). MiRNAs are crucial in the periodic development of hair follicles; they maintain epidermal homeostasis by targeting genes and influencing the activity of signaling pathways and related regulators. Our study discovered miR-129-5p to be overexpressed in the skin of Angora rabbits during catagen, and was negatively correlated with <i<HOXC13</i< expression (Pearson’s R <i<=</i< −0.313, <i<p</i< < 0.05). The dual-Luciferase reporter gene detection system and Western blotting confirmed that miR-129-5p targeted <i<HOXC13</i<. In addition, miR-129-5p overexpression was found to significantly inhibit the expression of hair follicle development-related genes (HFDRGs), such as <i<BCL2</i<, <i<WNT2</i<, <i<CCND1,</i< and <i<LEF1</i< (<i<p</i< < 0.01), and promoted the expression of <i<SFRP2</i<, <i<TGF-β1,</i< and <i<FGF2</i< (<i<p</i< < 0.01), which was the same as the knockdown of <i<HOXC13</i<. In contrast, the knockout of miR-129-5p was the opposite, and it demonstrated similar results to the overexpression of <i<HOXC13</i<. CCK8 and flow cytometry demonstrated that miR-129-5p mimics significantly promoted the apoptosis of dermal papilla cells (DPCs) and inhibited proliferation (<i<p</i< < 0.01), while the inhibitor was found to reduce the apoptosis of DPCs and promote proliferation (<i<p</i< < 0.01). These results showed that miR-129-5p can participate in the periodic development of HF by targeting <i<HOXC13,</i< and it can induce apoptosis and inhibit proliferation of DPCs. These results will help to understand the role and mechanism of miR-129-5p in the periodic development of HF, and will provide support for subsequent studies, not only providing a theoretical basis for genetically improving the quality of hair in animals in the future, but also a new theory and method for diagnosing and treating hair loss in humans.
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700	0		\|a Xinsheng Wu \|e verfasserin \|4 aut
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allfields	10.3390/genes13040679 doi (DE-627)DOAJ029887119 (DE-599)DOAJ1044b253bb944d54bd875338d832ba43 DE-627 ger DE-627 rakwb eng QH426-470 Fan Yao verfasserin aut miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Mammalian hair formation is critically determined by the growth of hair follicles (HF). MiRNAs are crucial in the periodic development of hair follicles; they maintain epidermal homeostasis by targeting genes and influencing the activity of signaling pathways and related regulators. Our study discovered miR-129-5p to be overexpressed in the skin of Angora rabbits during catagen, and was negatively correlated with <i<HOXC13</i< expression (Pearson’s R <i<=</i< −0.313, <i<p</i< < 0.05). The dual-Luciferase reporter gene detection system and Western blotting confirmed that miR-129-5p targeted <i<HOXC13</i<. In addition, miR-129-5p overexpression was found to significantly inhibit the expression of hair follicle development-related genes (HFDRGs), such as <i<BCL2</i<, <i<WNT2</i<, <i<CCND1,</i< and <i<LEF1</i< (<i<p</i< < 0.01), and promoted the expression of <i<SFRP2</i<, <i<TGF-β1,</i< and <i<FGF2</i< (<i<p</i< < 0.01), which was the same as the knockdown of <i<HOXC13</i<. In contrast, the knockout of miR-129-5p was the opposite, and it demonstrated similar results to the overexpression of <i<HOXC13</i<. CCK8 and flow cytometry demonstrated that miR-129-5p mimics significantly promoted the apoptosis of dermal papilla cells (DPCs) and inhibited proliferation (<i<p</i< < 0.01), while the inhibitor was found to reduce the apoptosis of DPCs and promote proliferation (<i<p</i< < 0.01). These results showed that miR-129-5p can participate in the periodic development of HF by targeting <i<HOXC13,</i< and it can induce apoptosis and inhibit proliferation of DPCs. These results will help to understand the role and mechanism of miR-129-5p in the periodic development of HF, and will provide support for subsequent studies, not only providing a theoretical basis for genetically improving the quality of hair in animals in the future, but also a new theory and method for diagnosing and treating hair loss in humans. miR-129-5p <i<HOXC13</i< DPCs proliferation and apoptosis Genetics Bohao Zhao verfasserin aut Shuaishuai Hu verfasserin aut Shaocheng Bai verfasserin aut Rongshuai Jin verfasserin aut Chen Zhang verfasserin aut Yang Chen verfasserin aut Xinsheng Wu verfasserin aut In Genes MDPI AG, 2010 13(2022), 4, p 679 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:4, p 679 https://doi.org/10.3390/genes13040679 kostenfrei https://doaj.org/article/1044b253bb944d54bd875338d832ba43 kostenfrei https://www.mdpi.com/2073-4425/13/4/679 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 4, p 679
spelling	10.3390/genes13040679 doi (DE-627)DOAJ029887119 (DE-599)DOAJ1044b253bb944d54bd875338d832ba43 DE-627 ger DE-627 rakwb eng QH426-470 Fan Yao verfasserin aut miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Mammalian hair formation is critically determined by the growth of hair follicles (HF). MiRNAs are crucial in the periodic development of hair follicles; they maintain epidermal homeostasis by targeting genes and influencing the activity of signaling pathways and related regulators. Our study discovered miR-129-5p to be overexpressed in the skin of Angora rabbits during catagen, and was negatively correlated with <i<HOXC13</i< expression (Pearson’s R <i<=</i< −0.313, <i<p</i< < 0.05). The dual-Luciferase reporter gene detection system and Western blotting confirmed that miR-129-5p targeted <i<HOXC13</i<. In addition, miR-129-5p overexpression was found to significantly inhibit the expression of hair follicle development-related genes (HFDRGs), such as <i<BCL2</i<, <i<WNT2</i<, <i<CCND1,</i< and <i<LEF1</i< (<i<p</i< < 0.01), and promoted the expression of <i<SFRP2</i<, <i<TGF-β1,</i< and <i<FGF2</i< (<i<p</i< < 0.01), which was the same as the knockdown of <i<HOXC13</i<. In contrast, the knockout of miR-129-5p was the opposite, and it demonstrated similar results to the overexpression of <i<HOXC13</i<. CCK8 and flow cytometry demonstrated that miR-129-5p mimics significantly promoted the apoptosis of dermal papilla cells (DPCs) and inhibited proliferation (<i<p</i< < 0.01), while the inhibitor was found to reduce the apoptosis of DPCs and promote proliferation (<i<p</i< < 0.01). These results showed that miR-129-5p can participate in the periodic development of HF by targeting <i<HOXC13,</i< and it can induce apoptosis and inhibit proliferation of DPCs. These results will help to understand the role and mechanism of miR-129-5p in the periodic development of HF, and will provide support for subsequent studies, not only providing a theoretical basis for genetically improving the quality of hair in animals in the future, but also a new theory and method for diagnosing and treating hair loss in humans. miR-129-5p <i<HOXC13</i< DPCs proliferation and apoptosis Genetics Bohao Zhao verfasserin aut Shuaishuai Hu verfasserin aut Shaocheng Bai verfasserin aut Rongshuai Jin verfasserin aut Chen Zhang verfasserin aut Yang Chen verfasserin aut Xinsheng Wu verfasserin aut In Genes MDPI AG, 2010 13(2022), 4, p 679 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:4, p 679 https://doi.org/10.3390/genes13040679 kostenfrei https://doaj.org/article/1044b253bb944d54bd875338d832ba43 kostenfrei https://www.mdpi.com/2073-4425/13/4/679 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 4, p 679
allfields_unstemmed	10.3390/genes13040679 doi (DE-627)DOAJ029887119 (DE-599)DOAJ1044b253bb944d54bd875338d832ba43 DE-627 ger DE-627 rakwb eng QH426-470 Fan Yao verfasserin aut miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Mammalian hair formation is critically determined by the growth of hair follicles (HF). MiRNAs are crucial in the periodic development of hair follicles; they maintain epidermal homeostasis by targeting genes and influencing the activity of signaling pathways and related regulators. Our study discovered miR-129-5p to be overexpressed in the skin of Angora rabbits during catagen, and was negatively correlated with <i<HOXC13</i< expression (Pearson’s R <i<=</i< −0.313, <i<p</i< < 0.05). The dual-Luciferase reporter gene detection system and Western blotting confirmed that miR-129-5p targeted <i<HOXC13</i<. In addition, miR-129-5p overexpression was found to significantly inhibit the expression of hair follicle development-related genes (HFDRGs), such as <i<BCL2</i<, <i<WNT2</i<, <i<CCND1,</i< and <i<LEF1</i< (<i<p</i< < 0.01), and promoted the expression of <i<SFRP2</i<, <i<TGF-β1,</i< and <i<FGF2</i< (<i<p</i< < 0.01), which was the same as the knockdown of <i<HOXC13</i<. In contrast, the knockout of miR-129-5p was the opposite, and it demonstrated similar results to the overexpression of <i<HOXC13</i<. CCK8 and flow cytometry demonstrated that miR-129-5p mimics significantly promoted the apoptosis of dermal papilla cells (DPCs) and inhibited proliferation (<i<p</i< < 0.01), while the inhibitor was found to reduce the apoptosis of DPCs and promote proliferation (<i<p</i< < 0.01). These results showed that miR-129-5p can participate in the periodic development of HF by targeting <i<HOXC13,</i< and it can induce apoptosis and inhibit proliferation of DPCs. These results will help to understand the role and mechanism of miR-129-5p in the periodic development of HF, and will provide support for subsequent studies, not only providing a theoretical basis for genetically improving the quality of hair in animals in the future, but also a new theory and method for diagnosing and treating hair loss in humans. miR-129-5p <i<HOXC13</i< DPCs proliferation and apoptosis Genetics Bohao Zhao verfasserin aut Shuaishuai Hu verfasserin aut Shaocheng Bai verfasserin aut Rongshuai Jin verfasserin aut Chen Zhang verfasserin aut Yang Chen verfasserin aut Xinsheng Wu verfasserin aut In Genes MDPI AG, 2010 13(2022), 4, p 679 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:4, p 679 https://doi.org/10.3390/genes13040679 kostenfrei https://doaj.org/article/1044b253bb944d54bd875338d832ba43 kostenfrei https://www.mdpi.com/2073-4425/13/4/679 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 4, p 679
allfieldsGer	10.3390/genes13040679 doi (DE-627)DOAJ029887119 (DE-599)DOAJ1044b253bb944d54bd875338d832ba43 DE-627 ger DE-627 rakwb eng QH426-470 Fan Yao verfasserin aut miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Mammalian hair formation is critically determined by the growth of hair follicles (HF). MiRNAs are crucial in the periodic development of hair follicles; they maintain epidermal homeostasis by targeting genes and influencing the activity of signaling pathways and related regulators. Our study discovered miR-129-5p to be overexpressed in the skin of Angora rabbits during catagen, and was negatively correlated with <i<HOXC13</i< expression (Pearson’s R <i<=</i< −0.313, <i<p</i< < 0.05). The dual-Luciferase reporter gene detection system and Western blotting confirmed that miR-129-5p targeted <i<HOXC13</i<. In addition, miR-129-5p overexpression was found to significantly inhibit the expression of hair follicle development-related genes (HFDRGs), such as <i<BCL2</i<, <i<WNT2</i<, <i<CCND1,</i< and <i<LEF1</i< (<i<p</i< < 0.01), and promoted the expression of <i<SFRP2</i<, <i<TGF-β1,</i< and <i<FGF2</i< (<i<p</i< < 0.01), which was the same as the knockdown of <i<HOXC13</i<. In contrast, the knockout of miR-129-5p was the opposite, and it demonstrated similar results to the overexpression of <i<HOXC13</i<. CCK8 and flow cytometry demonstrated that miR-129-5p mimics significantly promoted the apoptosis of dermal papilla cells (DPCs) and inhibited proliferation (<i<p</i< < 0.01), while the inhibitor was found to reduce the apoptosis of DPCs and promote proliferation (<i<p</i< < 0.01). These results showed that miR-129-5p can participate in the periodic development of HF by targeting <i<HOXC13,</i< and it can induce apoptosis and inhibit proliferation of DPCs. These results will help to understand the role and mechanism of miR-129-5p in the periodic development of HF, and will provide support for subsequent studies, not only providing a theoretical basis for genetically improving the quality of hair in animals in the future, but also a new theory and method for diagnosing and treating hair loss in humans. miR-129-5p <i<HOXC13</i< DPCs proliferation and apoptosis Genetics Bohao Zhao verfasserin aut Shuaishuai Hu verfasserin aut Shaocheng Bai verfasserin aut Rongshuai Jin verfasserin aut Chen Zhang verfasserin aut Yang Chen verfasserin aut Xinsheng Wu verfasserin aut In Genes MDPI AG, 2010 13(2022), 4, p 679 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:4, p 679 https://doi.org/10.3390/genes13040679 kostenfrei https://doaj.org/article/1044b253bb944d54bd875338d832ba43 kostenfrei https://www.mdpi.com/2073-4425/13/4/679 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 4, p 679
allfieldsSound	10.3390/genes13040679 doi (DE-627)DOAJ029887119 (DE-599)DOAJ1044b253bb944d54bd875338d832ba43 DE-627 ger DE-627 rakwb eng QH426-470 Fan Yao verfasserin aut miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Mammalian hair formation is critically determined by the growth of hair follicles (HF). MiRNAs are crucial in the periodic development of hair follicles; they maintain epidermal homeostasis by targeting genes and influencing the activity of signaling pathways and related regulators. Our study discovered miR-129-5p to be overexpressed in the skin of Angora rabbits during catagen, and was negatively correlated with <i<HOXC13</i< expression (Pearson’s R <i<=</i< −0.313, <i<p</i< < 0.05). The dual-Luciferase reporter gene detection system and Western blotting confirmed that miR-129-5p targeted <i<HOXC13</i<. In addition, miR-129-5p overexpression was found to significantly inhibit the expression of hair follicle development-related genes (HFDRGs), such as <i<BCL2</i<, <i<WNT2</i<, <i<CCND1,</i< and <i<LEF1</i< (<i<p</i< < 0.01), and promoted the expression of <i<SFRP2</i<, <i<TGF-β1,</i< and <i<FGF2</i< (<i<p</i< < 0.01), which was the same as the knockdown of <i<HOXC13</i<. In contrast, the knockout of miR-129-5p was the opposite, and it demonstrated similar results to the overexpression of <i<HOXC13</i<. CCK8 and flow cytometry demonstrated that miR-129-5p mimics significantly promoted the apoptosis of dermal papilla cells (DPCs) and inhibited proliferation (<i<p</i< < 0.01), while the inhibitor was found to reduce the apoptosis of DPCs and promote proliferation (<i<p</i< < 0.01). These results showed that miR-129-5p can participate in the periodic development of HF by targeting <i<HOXC13,</i< and it can induce apoptosis and inhibit proliferation of DPCs. These results will help to understand the role and mechanism of miR-129-5p in the periodic development of HF, and will provide support for subsequent studies, not only providing a theoretical basis for genetically improving the quality of hair in animals in the future, but also a new theory and method for diagnosing and treating hair loss in humans. miR-129-5p <i<HOXC13</i< DPCs proliferation and apoptosis Genetics Bohao Zhao verfasserin aut Shuaishuai Hu verfasserin aut Shaocheng Bai verfasserin aut Rongshuai Jin verfasserin aut Chen Zhang verfasserin aut Yang Chen verfasserin aut Xinsheng Wu verfasserin aut In Genes MDPI AG, 2010 13(2022), 4, p 679 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:4, p 679 https://doi.org/10.3390/genes13040679 kostenfrei https://doaj.org/article/1044b253bb944d54bd875338d832ba43 kostenfrei https://www.mdpi.com/2073-4425/13/4/679 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 4, p 679
language	English
source	In Genes 13(2022), 4, p 679 volume:13 year:2022 number:4, p 679
sourceStr	In Genes 13(2022), 4, p 679 volume:13 year:2022 number:4, p 679
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	miR-129-5p <i<HOXC13</i< DPCs proliferation and apoptosis Genetics
isfreeaccess_bool	true
container_title	Genes
authorswithroles_txt_mv	Fan Yao @@aut@@ Bohao Zhao @@aut@@ Shuaishuai Hu @@aut@@ Shaocheng Bai @@aut@@ Rongshuai Jin @@aut@@ Chen Zhang @@aut@@ Yang Chen @@aut@@ Xinsheng Wu @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
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callnumber-first	Q - Science
author	Fan Yao
spellingShingle	Fan Yao misc QH426-470 misc miR-129-5p misc <i<HOXC13</i< misc DPCs misc proliferation and apoptosis misc Genetics miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit
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topic_title	QH426-470 miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit miR-129-5p <i<HOXC13</i< DPCs proliferation and apoptosis
topic	misc QH426-470 misc miR-129-5p misc <i<HOXC13</i< misc DPCs misc proliferation and apoptosis misc Genetics
topic_unstemmed	misc QH426-470 misc miR-129-5p misc <i<HOXC13</i< misc DPCs misc proliferation and apoptosis misc Genetics
topic_browse	misc QH426-470 misc miR-129-5p misc <i<HOXC13</i< misc DPCs misc proliferation and apoptosis misc Genetics
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title	miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit
ctrlnum	(DE-627)DOAJ029887119 (DE-599)DOAJ1044b253bb944d54bd875338d832ba43
title_full	miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit
author_sort	Fan Yao
journal	Genes
journalStr	Genes
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lang_code	eng
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publishDateSort	2022
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author_browse	Fan Yao Bohao Zhao Shuaishuai Hu Shaocheng Bai Rongshuai Jin Chen Zhang Yang Chen Xinsheng Wu
container_volume	13
class	QH426-470
format_se	Elektronische Aufsätze
author-letter	Fan Yao
doi_str_mv	10.3390/genes13040679
author2-role	verfasserin
title_sort	mir-129-5p participates in hair follicle growth by targeting hoxc13 in rabbit
callnumber	QH426-470
title_auth	miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit
abstract	Mammalian hair formation is critically determined by the growth of hair follicles (HF). MiRNAs are crucial in the periodic development of hair follicles; they maintain epidermal homeostasis by targeting genes and influencing the activity of signaling pathways and related regulators. Our study discovered miR-129-5p to be overexpressed in the skin of Angora rabbits during catagen, and was negatively correlated with <i<HOXC13</i< expression (Pearson’s R <i<=</i< −0.313, <i<p</i< < 0.05). The dual-Luciferase reporter gene detection system and Western blotting confirmed that miR-129-5p targeted <i<HOXC13</i<. In addition, miR-129-5p overexpression was found to significantly inhibit the expression of hair follicle development-related genes (HFDRGs), such as <i<BCL2</i<, <i<WNT2</i<, <i<CCND1,</i< and <i<LEF1</i< (<i<p</i< < 0.01), and promoted the expression of <i<SFRP2</i<, <i<TGF-β1,</i< and <i<FGF2</i< (<i<p</i< < 0.01), which was the same as the knockdown of <i<HOXC13</i<. In contrast, the knockout of miR-129-5p was the opposite, and it demonstrated similar results to the overexpression of <i<HOXC13</i<. CCK8 and flow cytometry demonstrated that miR-129-5p mimics significantly promoted the apoptosis of dermal papilla cells (DPCs) and inhibited proliferation (<i<p</i< < 0.01), while the inhibitor was found to reduce the apoptosis of DPCs and promote proliferation (<i<p</i< < 0.01). These results showed that miR-129-5p can participate in the periodic development of HF by targeting <i<HOXC13,</i< and it can induce apoptosis and inhibit proliferation of DPCs. These results will help to understand the role and mechanism of miR-129-5p in the periodic development of HF, and will provide support for subsequent studies, not only providing a theoretical basis for genetically improving the quality of hair in animals in the future, but also a new theory and method for diagnosing and treating hair loss in humans.
abstractGer	Mammalian hair formation is critically determined by the growth of hair follicles (HF). MiRNAs are crucial in the periodic development of hair follicles; they maintain epidermal homeostasis by targeting genes and influencing the activity of signaling pathways and related regulators. Our study discovered miR-129-5p to be overexpressed in the skin of Angora rabbits during catagen, and was negatively correlated with <i<HOXC13</i< expression (Pearson’s R <i<=</i< −0.313, <i<p</i< < 0.05). The dual-Luciferase reporter gene detection system and Western blotting confirmed that miR-129-5p targeted <i<HOXC13</i<. In addition, miR-129-5p overexpression was found to significantly inhibit the expression of hair follicle development-related genes (HFDRGs), such as <i<BCL2</i<, <i<WNT2</i<, <i<CCND1,</i< and <i<LEF1</i< (<i<p</i< < 0.01), and promoted the expression of <i<SFRP2</i<, <i<TGF-β1,</i< and <i<FGF2</i< (<i<p</i< < 0.01), which was the same as the knockdown of <i<HOXC13</i<. In contrast, the knockout of miR-129-5p was the opposite, and it demonstrated similar results to the overexpression of <i<HOXC13</i<. CCK8 and flow cytometry demonstrated that miR-129-5p mimics significantly promoted the apoptosis of dermal papilla cells (DPCs) and inhibited proliferation (<i<p</i< < 0.01), while the inhibitor was found to reduce the apoptosis of DPCs and promote proliferation (<i<p</i< < 0.01). These results showed that miR-129-5p can participate in the periodic development of HF by targeting <i<HOXC13,</i< and it can induce apoptosis and inhibit proliferation of DPCs. These results will help to understand the role and mechanism of miR-129-5p in the periodic development of HF, and will provide support for subsequent studies, not only providing a theoretical basis for genetically improving the quality of hair in animals in the future, but also a new theory and method for diagnosing and treating hair loss in humans.
abstract_unstemmed	Mammalian hair formation is critically determined by the growth of hair follicles (HF). MiRNAs are crucial in the periodic development of hair follicles; they maintain epidermal homeostasis by targeting genes and influencing the activity of signaling pathways and related regulators. Our study discovered miR-129-5p to be overexpressed in the skin of Angora rabbits during catagen, and was negatively correlated with <i<HOXC13</i< expression (Pearson’s R <i<=</i< −0.313, <i<p</i< < 0.05). The dual-Luciferase reporter gene detection system and Western blotting confirmed that miR-129-5p targeted <i<HOXC13</i<. In addition, miR-129-5p overexpression was found to significantly inhibit the expression of hair follicle development-related genes (HFDRGs), such as <i<BCL2</i<, <i<WNT2</i<, <i<CCND1,</i< and <i<LEF1</i< (<i<p</i< < 0.01), and promoted the expression of <i<SFRP2</i<, <i<TGF-β1,</i< and <i<FGF2</i< (<i<p</i< < 0.01), which was the same as the knockdown of <i<HOXC13</i<. In contrast, the knockout of miR-129-5p was the opposite, and it demonstrated similar results to the overexpression of <i<HOXC13</i<. CCK8 and flow cytometry demonstrated that miR-129-5p mimics significantly promoted the apoptosis of dermal papilla cells (DPCs) and inhibited proliferation (<i<p</i< < 0.01), while the inhibitor was found to reduce the apoptosis of DPCs and promote proliferation (<i<p</i< < 0.01). These results showed that miR-129-5p can participate in the periodic development of HF by targeting <i<HOXC13,</i< and it can induce apoptosis and inhibit proliferation of DPCs. These results will help to understand the role and mechanism of miR-129-5p in the periodic development of HF, and will provide support for subsequent studies, not only providing a theoretical basis for genetically improving the quality of hair in animals in the future, but also a new theory and method for diagnosing and treating hair loss in humans.
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container_issue	4, p 679
title_short	miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit
url	https://doi.org/10.3390/genes13040679 https://doaj.org/article/1044b253bb944d54bd875338d832ba43 https://www.mdpi.com/2073-4425/13/4/679 https://doaj.org/toc/2073-4425
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author2	Bohao Zhao Shuaishuai Hu Shaocheng Bai Rongshuai Jin Chen Zhang Yang Chen Xinsheng Wu
author2Str	Bohao Zhao Shuaishuai Hu Shaocheng Bai Rongshuai Jin Chen Zhang Yang Chen Xinsheng Wu
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doi_str	10.3390/genes13040679
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up_date	2024-07-04T00:48:01.232Z
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