Comparison of Different In Situ Hybridization Techniques for the Detection of Various RNA and DNA Viruses

In situ hybridization (ISH) is a technique to determine potential correlations between viruses and lesions. The aim of the study was to compare ISH techniques for the detection of various viruses in different tissues. Tested RNA viruses include atypical porcine pestivirus (APPV) in the cerebellum of...
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Autor*in:	Vanessa M. Pfankuche [verfasserIn] Kerstin Hahn [verfasserIn] Rogier Bodewes [verfasserIn] Florian Hansmann [verfasserIn] André Habierski [verfasserIn] Ann-Kathrin Haverkamp [verfasserIn] Stephanie Pfaender [verfasserIn] Stephanie Walter [verfasserIn] Christine Baechlein [verfasserIn] Alexander Postel [verfasserIn] Eike Steinmann [verfasserIn] Paul Becher [verfasserIn] Albert Osterhaus [verfasserIn] Wolfgang Baumgärtner [verfasserIn] Christina Puff [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	chromogenic in situ hybridization digoxigenin DNA virus fast red fluorescent in situ hybridization RNA virus virus discovery

Übergeordnetes Werk:	In: Viruses - MDPI AG, 2009, 10(2018), 7, p 384
Übergeordnetes Werk:	volume:10 ; year:2018 ; number:7, p 384

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/v10070384

Katalog-ID:	DOAJ029908752

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allfields	10.3390/v10070384 doi (DE-627)DOAJ029908752 (DE-599)DOAJce9e4f25160f46bfa714cc2053a13bc3 DE-627 ger DE-627 rakwb eng QR1-502 Vanessa M. Pfankuche verfasserin aut Comparison of Different In Situ Hybridization Techniques for the Detection of Various RNA and DNA Viruses 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In situ hybridization (ISH) is a technique to determine potential correlations between viruses and lesions. The aim of the study was to compare ISH techniques for the detection of various viruses in different tissues. Tested RNA viruses include atypical porcine pestivirus (APPV) in the cerebellum of pigs, equine and bovine hepacivirus (EqHV, BovHepV) in the liver of horses and cattle, respectively, and Schmallenberg virus (SBV) in the cerebrum of goats. Examined DNA viruses comprise canine bocavirus 2 (CBoV-2) in the intestine of dogs, porcine bocavirus (PBoV) in the spinal cord of pigs and porcine circovirus 2 (PCV-2) in cerebrum, lymph node, and lung of pigs. ISH with self-designed digoxigenin-labelled RNA probes revealed a positive signal for SBV, CBoV-2, and PCV-2, whereas it was lacking for APPV, BovHepV, EqHV, and PBoV. Commercially produced digoxigenin-labelled DNA probes detected CBoV-2 and PCV-2, but failed to detect PBoV. ISH with a commercially available fluorescent ISH (FISH)-RNA probe mix identified nucleic acids of all tested viruses. The detection rate and the cell-associated positive area using the FISH-RNA probe mix was highest compared to the results using other probes and protocols, representing a major benefit of this method. Nevertheless, there are differences in costs and procedure time. chromogenic in situ hybridization digoxigenin DNA virus fast red fluorescent in situ hybridization RNA virus virus discovery Microbiology Kerstin Hahn verfasserin aut Rogier Bodewes verfasserin aut Florian Hansmann verfasserin aut André Habierski verfasserin aut Ann-Kathrin Haverkamp verfasserin aut Stephanie Pfaender verfasserin aut Stephanie Walter verfasserin aut Christine Baechlein verfasserin aut Alexander Postel verfasserin aut Eike Steinmann verfasserin aut Paul Becher verfasserin aut Albert Osterhaus verfasserin aut Wolfgang Baumgärtner verfasserin aut Christina Puff verfasserin aut In Viruses MDPI AG, 2009 10(2018), 7, p 384 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:10 year:2018 number:7, p 384 https://doi.org/10.3390/v10070384 kostenfrei https://doaj.org/article/ce9e4f25160f46bfa714cc2053a13bc3 kostenfrei http://www.mdpi.com/1999-4915/10/7/384 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2018 7, p 384
spelling	10.3390/v10070384 doi (DE-627)DOAJ029908752 (DE-599)DOAJce9e4f25160f46bfa714cc2053a13bc3 DE-627 ger DE-627 rakwb eng QR1-502 Vanessa M. Pfankuche verfasserin aut Comparison of Different In Situ Hybridization Techniques for the Detection of Various RNA and DNA Viruses 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In situ hybridization (ISH) is a technique to determine potential correlations between viruses and lesions. The aim of the study was to compare ISH techniques for the detection of various viruses in different tissues. Tested RNA viruses include atypical porcine pestivirus (APPV) in the cerebellum of pigs, equine and bovine hepacivirus (EqHV, BovHepV) in the liver of horses and cattle, respectively, and Schmallenberg virus (SBV) in the cerebrum of goats. Examined DNA viruses comprise canine bocavirus 2 (CBoV-2) in the intestine of dogs, porcine bocavirus (PBoV) in the spinal cord of pigs and porcine circovirus 2 (PCV-2) in cerebrum, lymph node, and lung of pigs. ISH with self-designed digoxigenin-labelled RNA probes revealed a positive signal for SBV, CBoV-2, and PCV-2, whereas it was lacking for APPV, BovHepV, EqHV, and PBoV. Commercially produced digoxigenin-labelled DNA probes detected CBoV-2 and PCV-2, but failed to detect PBoV. ISH with a commercially available fluorescent ISH (FISH)-RNA probe mix identified nucleic acids of all tested viruses. The detection rate and the cell-associated positive area using the FISH-RNA probe mix was highest compared to the results using other probes and protocols, representing a major benefit of this method. Nevertheless, there are differences in costs and procedure time. chromogenic in situ hybridization digoxigenin DNA virus fast red fluorescent in situ hybridization RNA virus virus discovery Microbiology Kerstin Hahn verfasserin aut Rogier Bodewes verfasserin aut Florian Hansmann verfasserin aut André Habierski verfasserin aut Ann-Kathrin Haverkamp verfasserin aut Stephanie Pfaender verfasserin aut Stephanie Walter verfasserin aut Christine Baechlein verfasserin aut Alexander Postel verfasserin aut Eike Steinmann verfasserin aut Paul Becher verfasserin aut Albert Osterhaus verfasserin aut Wolfgang Baumgärtner verfasserin aut Christina Puff verfasserin aut In Viruses MDPI AG, 2009 10(2018), 7, p 384 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:10 year:2018 number:7, p 384 https://doi.org/10.3390/v10070384 kostenfrei https://doaj.org/article/ce9e4f25160f46bfa714cc2053a13bc3 kostenfrei http://www.mdpi.com/1999-4915/10/7/384 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2018 7, p 384
allfields_unstemmed	10.3390/v10070384 doi (DE-627)DOAJ029908752 (DE-599)DOAJce9e4f25160f46bfa714cc2053a13bc3 DE-627 ger DE-627 rakwb eng QR1-502 Vanessa M. Pfankuche verfasserin aut Comparison of Different In Situ Hybridization Techniques for the Detection of Various RNA and DNA Viruses 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In situ hybridization (ISH) is a technique to determine potential correlations between viruses and lesions. The aim of the study was to compare ISH techniques for the detection of various viruses in different tissues. Tested RNA viruses include atypical porcine pestivirus (APPV) in the cerebellum of pigs, equine and bovine hepacivirus (EqHV, BovHepV) in the liver of horses and cattle, respectively, and Schmallenberg virus (SBV) in the cerebrum of goats. Examined DNA viruses comprise canine bocavirus 2 (CBoV-2) in the intestine of dogs, porcine bocavirus (PBoV) in the spinal cord of pigs and porcine circovirus 2 (PCV-2) in cerebrum, lymph node, and lung of pigs. ISH with self-designed digoxigenin-labelled RNA probes revealed a positive signal for SBV, CBoV-2, and PCV-2, whereas it was lacking for APPV, BovHepV, EqHV, and PBoV. Commercially produced digoxigenin-labelled DNA probes detected CBoV-2 and PCV-2, but failed to detect PBoV. ISH with a commercially available fluorescent ISH (FISH)-RNA probe mix identified nucleic acids of all tested viruses. The detection rate and the cell-associated positive area using the FISH-RNA probe mix was highest compared to the results using other probes and protocols, representing a major benefit of this method. Nevertheless, there are differences in costs and procedure time. chromogenic in situ hybridization digoxigenin DNA virus fast red fluorescent in situ hybridization RNA virus virus discovery Microbiology Kerstin Hahn verfasserin aut Rogier Bodewes verfasserin aut Florian Hansmann verfasserin aut André Habierski verfasserin aut Ann-Kathrin Haverkamp verfasserin aut Stephanie Pfaender verfasserin aut Stephanie Walter verfasserin aut Christine Baechlein verfasserin aut Alexander Postel verfasserin aut Eike Steinmann verfasserin aut Paul Becher verfasserin aut Albert Osterhaus verfasserin aut Wolfgang Baumgärtner verfasserin aut Christina Puff verfasserin aut In Viruses MDPI AG, 2009 10(2018), 7, p 384 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:10 year:2018 number:7, p 384 https://doi.org/10.3390/v10070384 kostenfrei https://doaj.org/article/ce9e4f25160f46bfa714cc2053a13bc3 kostenfrei http://www.mdpi.com/1999-4915/10/7/384 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2018 7, p 384
allfieldsGer	10.3390/v10070384 doi (DE-627)DOAJ029908752 (DE-599)DOAJce9e4f25160f46bfa714cc2053a13bc3 DE-627 ger DE-627 rakwb eng QR1-502 Vanessa M. Pfankuche verfasserin aut Comparison of Different In Situ Hybridization Techniques for the Detection of Various RNA and DNA Viruses 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In situ hybridization (ISH) is a technique to determine potential correlations between viruses and lesions. The aim of the study was to compare ISH techniques for the detection of various viruses in different tissues. Tested RNA viruses include atypical porcine pestivirus (APPV) in the cerebellum of pigs, equine and bovine hepacivirus (EqHV, BovHepV) in the liver of horses and cattle, respectively, and Schmallenberg virus (SBV) in the cerebrum of goats. Examined DNA viruses comprise canine bocavirus 2 (CBoV-2) in the intestine of dogs, porcine bocavirus (PBoV) in the spinal cord of pigs and porcine circovirus 2 (PCV-2) in cerebrum, lymph node, and lung of pigs. ISH with self-designed digoxigenin-labelled RNA probes revealed a positive signal for SBV, CBoV-2, and PCV-2, whereas it was lacking for APPV, BovHepV, EqHV, and PBoV. Commercially produced digoxigenin-labelled DNA probes detected CBoV-2 and PCV-2, but failed to detect PBoV. ISH with a commercially available fluorescent ISH (FISH)-RNA probe mix identified nucleic acids of all tested viruses. The detection rate and the cell-associated positive area using the FISH-RNA probe mix was highest compared to the results using other probes and protocols, representing a major benefit of this method. Nevertheless, there are differences in costs and procedure time. chromogenic in situ hybridization digoxigenin DNA virus fast red fluorescent in situ hybridization RNA virus virus discovery Microbiology Kerstin Hahn verfasserin aut Rogier Bodewes verfasserin aut Florian Hansmann verfasserin aut André Habierski verfasserin aut Ann-Kathrin Haverkamp verfasserin aut Stephanie Pfaender verfasserin aut Stephanie Walter verfasserin aut Christine Baechlein verfasserin aut Alexander Postel verfasserin aut Eike Steinmann verfasserin aut Paul Becher verfasserin aut Albert Osterhaus verfasserin aut Wolfgang Baumgärtner verfasserin aut Christina Puff verfasserin aut In Viruses MDPI AG, 2009 10(2018), 7, p 384 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:10 year:2018 number:7, p 384 https://doi.org/10.3390/v10070384 kostenfrei https://doaj.org/article/ce9e4f25160f46bfa714cc2053a13bc3 kostenfrei http://www.mdpi.com/1999-4915/10/7/384 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2018 7, p 384
allfieldsSound	10.3390/v10070384 doi (DE-627)DOAJ029908752 (DE-599)DOAJce9e4f25160f46bfa714cc2053a13bc3 DE-627 ger DE-627 rakwb eng QR1-502 Vanessa M. Pfankuche verfasserin aut Comparison of Different In Situ Hybridization Techniques for the Detection of Various RNA and DNA Viruses 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In situ hybridization (ISH) is a technique to determine potential correlations between viruses and lesions. The aim of the study was to compare ISH techniques for the detection of various viruses in different tissues. Tested RNA viruses include atypical porcine pestivirus (APPV) in the cerebellum of pigs, equine and bovine hepacivirus (EqHV, BovHepV) in the liver of horses and cattle, respectively, and Schmallenberg virus (SBV) in the cerebrum of goats. Examined DNA viruses comprise canine bocavirus 2 (CBoV-2) in the intestine of dogs, porcine bocavirus (PBoV) in the spinal cord of pigs and porcine circovirus 2 (PCV-2) in cerebrum, lymph node, and lung of pigs. ISH with self-designed digoxigenin-labelled RNA probes revealed a positive signal for SBV, CBoV-2, and PCV-2, whereas it was lacking for APPV, BovHepV, EqHV, and PBoV. Commercially produced digoxigenin-labelled DNA probes detected CBoV-2 and PCV-2, but failed to detect PBoV. ISH with a commercially available fluorescent ISH (FISH)-RNA probe mix identified nucleic acids of all tested viruses. The detection rate and the cell-associated positive area using the FISH-RNA probe mix was highest compared to the results using other probes and protocols, representing a major benefit of this method. Nevertheless, there are differences in costs and procedure time. chromogenic in situ hybridization digoxigenin DNA virus fast red fluorescent in situ hybridization RNA virus virus discovery Microbiology Kerstin Hahn verfasserin aut Rogier Bodewes verfasserin aut Florian Hansmann verfasserin aut André Habierski verfasserin aut Ann-Kathrin Haverkamp verfasserin aut Stephanie Pfaender verfasserin aut Stephanie Walter verfasserin aut Christine Baechlein verfasserin aut Alexander Postel verfasserin aut Eike Steinmann verfasserin aut Paul Becher verfasserin aut Albert Osterhaus verfasserin aut Wolfgang Baumgärtner verfasserin aut Christina Puff verfasserin aut In Viruses MDPI AG, 2009 10(2018), 7, p 384 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:10 year:2018 number:7, p 384 https://doi.org/10.3390/v10070384 kostenfrei https://doaj.org/article/ce9e4f25160f46bfa714cc2053a13bc3 kostenfrei http://www.mdpi.com/1999-4915/10/7/384 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2018 7, p 384
language	English
source	In Viruses 10(2018), 7, p 384 volume:10 year:2018 number:7, p 384
sourceStr	In Viruses 10(2018), 7, p 384 volume:10 year:2018 number:7, p 384
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	chromogenic in situ hybridization digoxigenin DNA virus fast red fluorescent in situ hybridization RNA virus virus discovery Microbiology
isfreeaccess_bool	true
container_title	Viruses
authorswithroles_txt_mv	Vanessa M. Pfankuche @@aut@@ Kerstin Hahn @@aut@@ Rogier Bodewes @@aut@@ Florian Hansmann @@aut@@ André Habierski @@aut@@ Ann-Kathrin Haverkamp @@aut@@ Stephanie Pfaender @@aut@@ Stephanie Walter @@aut@@ Christine Baechlein @@aut@@ Alexander Postel @@aut@@ Eike Steinmann @@aut@@ Paul Becher @@aut@@ Albert Osterhaus @@aut@@ Wolfgang Baumgärtner @@aut@@ Christina Puff @@aut@@
publishDateDaySort_date	2018-01-01T00:00:00Z
hierarchy_top_id	609775871
id	DOAJ029908752
language_de	englisch
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callnumber-first	Q - Science
author	Vanessa M. Pfankuche
spellingShingle	Vanessa M. Pfankuche misc QR1-502 misc chromogenic in situ hybridization misc digoxigenin misc DNA virus misc fast red misc fluorescent in situ hybridization misc RNA virus misc virus discovery misc Microbiology Comparison of Different In Situ Hybridization Techniques for the Detection of Various RNA and DNA Viruses
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topic_title	QR1-502 Comparison of Different In Situ Hybridization Techniques for the Detection of Various RNA and DNA Viruses chromogenic in situ hybridization digoxigenin DNA virus fast red fluorescent in situ hybridization RNA virus virus discovery
topic	misc QR1-502 misc chromogenic in situ hybridization misc digoxigenin misc DNA virus misc fast red misc fluorescent in situ hybridization misc RNA virus misc virus discovery misc Microbiology
topic_unstemmed	misc QR1-502 misc chromogenic in situ hybridization misc digoxigenin misc DNA virus misc fast red misc fluorescent in situ hybridization misc RNA virus misc virus discovery misc Microbiology
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title	Comparison of Different In Situ Hybridization Techniques for the Detection of Various RNA and DNA Viruses
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title_full	Comparison of Different In Situ Hybridization Techniques for the Detection of Various RNA and DNA Viruses
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author_browse	Vanessa M. Pfankuche Kerstin Hahn Rogier Bodewes Florian Hansmann André Habierski Ann-Kathrin Haverkamp Stephanie Pfaender Stephanie Walter Christine Baechlein Alexander Postel Eike Steinmann Paul Becher Albert Osterhaus Wolfgang Baumgärtner Christina Puff
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title_sort	comparison of different in situ hybridization techniques for the detection of various rna and dna viruses
callnumber	QR1-502
title_auth	Comparison of Different In Situ Hybridization Techniques for the Detection of Various RNA and DNA Viruses
abstract	In situ hybridization (ISH) is a technique to determine potential correlations between viruses and lesions. The aim of the study was to compare ISH techniques for the detection of various viruses in different tissues. Tested RNA viruses include atypical porcine pestivirus (APPV) in the cerebellum of pigs, equine and bovine hepacivirus (EqHV, BovHepV) in the liver of horses and cattle, respectively, and Schmallenberg virus (SBV) in the cerebrum of goats. Examined DNA viruses comprise canine bocavirus 2 (CBoV-2) in the intestine of dogs, porcine bocavirus (PBoV) in the spinal cord of pigs and porcine circovirus 2 (PCV-2) in cerebrum, lymph node, and lung of pigs. ISH with self-designed digoxigenin-labelled RNA probes revealed a positive signal for SBV, CBoV-2, and PCV-2, whereas it was lacking for APPV, BovHepV, EqHV, and PBoV. Commercially produced digoxigenin-labelled DNA probes detected CBoV-2 and PCV-2, but failed to detect PBoV. ISH with a commercially available fluorescent ISH (FISH)-RNA probe mix identified nucleic acids of all tested viruses. The detection rate and the cell-associated positive area using the FISH-RNA probe mix was highest compared to the results using other probes and protocols, representing a major benefit of this method. Nevertheless, there are differences in costs and procedure time.
abstractGer	In situ hybridization (ISH) is a technique to determine potential correlations between viruses and lesions. The aim of the study was to compare ISH techniques for the detection of various viruses in different tissues. Tested RNA viruses include atypical porcine pestivirus (APPV) in the cerebellum of pigs, equine and bovine hepacivirus (EqHV, BovHepV) in the liver of horses and cattle, respectively, and Schmallenberg virus (SBV) in the cerebrum of goats. Examined DNA viruses comprise canine bocavirus 2 (CBoV-2) in the intestine of dogs, porcine bocavirus (PBoV) in the spinal cord of pigs and porcine circovirus 2 (PCV-2) in cerebrum, lymph node, and lung of pigs. ISH with self-designed digoxigenin-labelled RNA probes revealed a positive signal for SBV, CBoV-2, and PCV-2, whereas it was lacking for APPV, BovHepV, EqHV, and PBoV. Commercially produced digoxigenin-labelled DNA probes detected CBoV-2 and PCV-2, but failed to detect PBoV. ISH with a commercially available fluorescent ISH (FISH)-RNA probe mix identified nucleic acids of all tested viruses. The detection rate and the cell-associated positive area using the FISH-RNA probe mix was highest compared to the results using other probes and protocols, representing a major benefit of this method. Nevertheless, there are differences in costs and procedure time.
abstract_unstemmed	In situ hybridization (ISH) is a technique to determine potential correlations between viruses and lesions. The aim of the study was to compare ISH techniques for the detection of various viruses in different tissues. Tested RNA viruses include atypical porcine pestivirus (APPV) in the cerebellum of pigs, equine and bovine hepacivirus (EqHV, BovHepV) in the liver of horses and cattle, respectively, and Schmallenberg virus (SBV) in the cerebrum of goats. Examined DNA viruses comprise canine bocavirus 2 (CBoV-2) in the intestine of dogs, porcine bocavirus (PBoV) in the spinal cord of pigs and porcine circovirus 2 (PCV-2) in cerebrum, lymph node, and lung of pigs. ISH with self-designed digoxigenin-labelled RNA probes revealed a positive signal for SBV, CBoV-2, and PCV-2, whereas it was lacking for APPV, BovHepV, EqHV, and PBoV. Commercially produced digoxigenin-labelled DNA probes detected CBoV-2 and PCV-2, but failed to detect PBoV. ISH with a commercially available fluorescent ISH (FISH)-RNA probe mix identified nucleic acids of all tested viruses. The detection rate and the cell-associated positive area using the FISH-RNA probe mix was highest compared to the results using other probes and protocols, representing a major benefit of this method. Nevertheless, there are differences in costs and procedure time.
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title_short	Comparison of Different In Situ Hybridization Techniques for the Detection of Various RNA and DNA Viruses
url	https://doi.org/10.3390/v10070384 https://doaj.org/article/ce9e4f25160f46bfa714cc2053a13bc3 http://www.mdpi.com/1999-4915/10/7/384 https://doaj.org/toc/1999-4915
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Comparison of Different In Situ Hybridization Techniques for the Detection of Various RNA and DNA Viruses

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