Development of Comprehensive Serological Techniques for Sensitive, Quantitative and Rapid Detection of <i<Soybean mosaic virus</i<

Soybean is an important grain and oil crop worldwide; however, the yield and seed quality of which are seriously affected by <i<Soybean mosaic virus</i< (SMV). As efficient detection technology is crucial for the field management of SMV, novel immunological detection methods were develop...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Rui Ren [verfasserIn] Tao Wang [verfasserIn] Le Gao [verfasserIn] Puwen Song [verfasserIn] Yunhua Yang [verfasserIn] Haijian Zhi [verfasserIn] Kai Li [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	SMV-CP PAb-SMV-CP MAb-SMV-CP DAS-qELISA SMV-GICS

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 23(2022), 16, p 9457
Übergeordnetes Werk:	volume:23 ; year:2022 ; number:16, p 9457

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms23169457

Katalog-ID:	DOAJ030312671

Internformat


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520			\|a Soybean is an important grain and oil crop worldwide; however, the yield and seed quality of which are seriously affected by <i<Soybean mosaic virus</i< (SMV). As efficient detection technology is crucial for the field management of SMV, novel immunological detection methods were developed in the present study. According to the phylogenetic analysis, the CP coding sequence of SMV-SC7 was selected for the prokaryotic expression of the recombinant SMV-CP. Purified SMV-CP was used for the development of polyclonal antibodies (PAb) against the SMV-CP (PAb-SMV-CP) and monoclonal antibodies (MAb) against SMV-CP (MAb-SMV-CP). Subsequently, the PAb-SMV-CP was used for the development of a novel DAS- quantitative ELISA (DAS-qELISA) kit, of which the sensitivity was greater than 1:4000, and this could be used for the quantitative detection of SMV in China. Meanwhile, the MAb-SMV-CP was labeled with colloidal gold, and then was used for the development of the SMV-specific gold immunochromatography strip (SMV-GICS). The SMV-GICS gives accurate detection results through observed control lines and test lines in 5 to 10 min, sharing the same sensitivity as RT-PCR, and can be used for rapid, accurate and high-throughput field SMV detection. The DAS-qELISA kit and the SMV-GICA strip developed in this study are SMV-specific, sensitive, cheap and easy to use. These products will be conducive to the timely, efficient SMV epidemiology and detection in major soybean-producing regions in China and abroad.
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912			\|a GBV_ILN_60
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Indexfelder

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allfields	10.3390/ijms23169457 doi (DE-627)DOAJ030312671 (DE-599)DOAJedab347900e94511b12c86a7f616443c DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Rui Ren verfasserin aut Development of Comprehensive Serological Techniques for Sensitive, Quantitative and Rapid Detection of <i<Soybean mosaic virus</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Soybean is an important grain and oil crop worldwide; however, the yield and seed quality of which are seriously affected by <i<Soybean mosaic virus</i< (SMV). As efficient detection technology is crucial for the field management of SMV, novel immunological detection methods were developed in the present study. According to the phylogenetic analysis, the CP coding sequence of SMV-SC7 was selected for the prokaryotic expression of the recombinant SMV-CP. Purified SMV-CP was used for the development of polyclonal antibodies (PAb) against the SMV-CP (PAb-SMV-CP) and monoclonal antibodies (MAb) against SMV-CP (MAb-SMV-CP). Subsequently, the PAb-SMV-CP was used for the development of a novel DAS- quantitative ELISA (DAS-qELISA) kit, of which the sensitivity was greater than 1:4000, and this could be used for the quantitative detection of SMV in China. Meanwhile, the MAb-SMV-CP was labeled with colloidal gold, and then was used for the development of the SMV-specific gold immunochromatography strip (SMV-GICS). The SMV-GICS gives accurate detection results through observed control lines and test lines in 5 to 10 min, sharing the same sensitivity as RT-PCR, and can be used for rapid, accurate and high-throughput field SMV detection. The DAS-qELISA kit and the SMV-GICA strip developed in this study are SMV-specific, sensitive, cheap and easy to use. These products will be conducive to the timely, efficient SMV epidemiology and detection in major soybean-producing regions in China and abroad. <i<Soybean mosaic virus</i< SMV-CP PAb-SMV-CP MAb-SMV-CP DAS-qELISA SMV-GICS Biology (General) Chemistry Tao Wang verfasserin aut Le Gao verfasserin aut Puwen Song verfasserin aut Yunhua Yang verfasserin aut Haijian Zhi verfasserin aut Kai Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 16, p 9457 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:16, p 9457 https://doi.org/10.3390/ijms23169457 kostenfrei https://doaj.org/article/edab347900e94511b12c86a7f616443c kostenfrei https://www.mdpi.com/1422-0067/23/16/9457 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 16, p 9457
spelling	10.3390/ijms23169457 doi (DE-627)DOAJ030312671 (DE-599)DOAJedab347900e94511b12c86a7f616443c DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Rui Ren verfasserin aut Development of Comprehensive Serological Techniques for Sensitive, Quantitative and Rapid Detection of <i<Soybean mosaic virus</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Soybean is an important grain and oil crop worldwide; however, the yield and seed quality of which are seriously affected by <i<Soybean mosaic virus</i< (SMV). As efficient detection technology is crucial for the field management of SMV, novel immunological detection methods were developed in the present study. According to the phylogenetic analysis, the CP coding sequence of SMV-SC7 was selected for the prokaryotic expression of the recombinant SMV-CP. Purified SMV-CP was used for the development of polyclonal antibodies (PAb) against the SMV-CP (PAb-SMV-CP) and monoclonal antibodies (MAb) against SMV-CP (MAb-SMV-CP). Subsequently, the PAb-SMV-CP was used for the development of a novel DAS- quantitative ELISA (DAS-qELISA) kit, of which the sensitivity was greater than 1:4000, and this could be used for the quantitative detection of SMV in China. Meanwhile, the MAb-SMV-CP was labeled with colloidal gold, and then was used for the development of the SMV-specific gold immunochromatography strip (SMV-GICS). The SMV-GICS gives accurate detection results through observed control lines and test lines in 5 to 10 min, sharing the same sensitivity as RT-PCR, and can be used for rapid, accurate and high-throughput field SMV detection. The DAS-qELISA kit and the SMV-GICA strip developed in this study are SMV-specific, sensitive, cheap and easy to use. These products will be conducive to the timely, efficient SMV epidemiology and detection in major soybean-producing regions in China and abroad. <i<Soybean mosaic virus</i< SMV-CP PAb-SMV-CP MAb-SMV-CP DAS-qELISA SMV-GICS Biology (General) Chemistry Tao Wang verfasserin aut Le Gao verfasserin aut Puwen Song verfasserin aut Yunhua Yang verfasserin aut Haijian Zhi verfasserin aut Kai Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 16, p 9457 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:16, p 9457 https://doi.org/10.3390/ijms23169457 kostenfrei https://doaj.org/article/edab347900e94511b12c86a7f616443c kostenfrei https://www.mdpi.com/1422-0067/23/16/9457 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 16, p 9457
allfields_unstemmed	10.3390/ijms23169457 doi (DE-627)DOAJ030312671 (DE-599)DOAJedab347900e94511b12c86a7f616443c DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Rui Ren verfasserin aut Development of Comprehensive Serological Techniques for Sensitive, Quantitative and Rapid Detection of <i<Soybean mosaic virus</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Soybean is an important grain and oil crop worldwide; however, the yield and seed quality of which are seriously affected by <i<Soybean mosaic virus</i< (SMV). As efficient detection technology is crucial for the field management of SMV, novel immunological detection methods were developed in the present study. According to the phylogenetic analysis, the CP coding sequence of SMV-SC7 was selected for the prokaryotic expression of the recombinant SMV-CP. Purified SMV-CP was used for the development of polyclonal antibodies (PAb) against the SMV-CP (PAb-SMV-CP) and monoclonal antibodies (MAb) against SMV-CP (MAb-SMV-CP). Subsequently, the PAb-SMV-CP was used for the development of a novel DAS- quantitative ELISA (DAS-qELISA) kit, of which the sensitivity was greater than 1:4000, and this could be used for the quantitative detection of SMV in China. Meanwhile, the MAb-SMV-CP was labeled with colloidal gold, and then was used for the development of the SMV-specific gold immunochromatography strip (SMV-GICS). The SMV-GICS gives accurate detection results through observed control lines and test lines in 5 to 10 min, sharing the same sensitivity as RT-PCR, and can be used for rapid, accurate and high-throughput field SMV detection. The DAS-qELISA kit and the SMV-GICA strip developed in this study are SMV-specific, sensitive, cheap and easy to use. These products will be conducive to the timely, efficient SMV epidemiology and detection in major soybean-producing regions in China and abroad. <i<Soybean mosaic virus</i< SMV-CP PAb-SMV-CP MAb-SMV-CP DAS-qELISA SMV-GICS Biology (General) Chemistry Tao Wang verfasserin aut Le Gao verfasserin aut Puwen Song verfasserin aut Yunhua Yang verfasserin aut Haijian Zhi verfasserin aut Kai Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 16, p 9457 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:16, p 9457 https://doi.org/10.3390/ijms23169457 kostenfrei https://doaj.org/article/edab347900e94511b12c86a7f616443c kostenfrei https://www.mdpi.com/1422-0067/23/16/9457 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 16, p 9457
allfieldsGer	10.3390/ijms23169457 doi (DE-627)DOAJ030312671 (DE-599)DOAJedab347900e94511b12c86a7f616443c DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Rui Ren verfasserin aut Development of Comprehensive Serological Techniques for Sensitive, Quantitative and Rapid Detection of <i<Soybean mosaic virus</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Soybean is an important grain and oil crop worldwide; however, the yield and seed quality of which are seriously affected by <i<Soybean mosaic virus</i< (SMV). As efficient detection technology is crucial for the field management of SMV, novel immunological detection methods were developed in the present study. According to the phylogenetic analysis, the CP coding sequence of SMV-SC7 was selected for the prokaryotic expression of the recombinant SMV-CP. Purified SMV-CP was used for the development of polyclonal antibodies (PAb) against the SMV-CP (PAb-SMV-CP) and monoclonal antibodies (MAb) against SMV-CP (MAb-SMV-CP). Subsequently, the PAb-SMV-CP was used for the development of a novel DAS- quantitative ELISA (DAS-qELISA) kit, of which the sensitivity was greater than 1:4000, and this could be used for the quantitative detection of SMV in China. Meanwhile, the MAb-SMV-CP was labeled with colloidal gold, and then was used for the development of the SMV-specific gold immunochromatography strip (SMV-GICS). The SMV-GICS gives accurate detection results through observed control lines and test lines in 5 to 10 min, sharing the same sensitivity as RT-PCR, and can be used for rapid, accurate and high-throughput field SMV detection. The DAS-qELISA kit and the SMV-GICA strip developed in this study are SMV-specific, sensitive, cheap and easy to use. These products will be conducive to the timely, efficient SMV epidemiology and detection in major soybean-producing regions in China and abroad. <i<Soybean mosaic virus</i< SMV-CP PAb-SMV-CP MAb-SMV-CP DAS-qELISA SMV-GICS Biology (General) Chemistry Tao Wang verfasserin aut Le Gao verfasserin aut Puwen Song verfasserin aut Yunhua Yang verfasserin aut Haijian Zhi verfasserin aut Kai Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 16, p 9457 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:16, p 9457 https://doi.org/10.3390/ijms23169457 kostenfrei https://doaj.org/article/edab347900e94511b12c86a7f616443c kostenfrei https://www.mdpi.com/1422-0067/23/16/9457 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 16, p 9457
allfieldsSound	10.3390/ijms23169457 doi (DE-627)DOAJ030312671 (DE-599)DOAJedab347900e94511b12c86a7f616443c DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Rui Ren verfasserin aut Development of Comprehensive Serological Techniques for Sensitive, Quantitative and Rapid Detection of <i<Soybean mosaic virus</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Soybean is an important grain and oil crop worldwide; however, the yield and seed quality of which are seriously affected by <i<Soybean mosaic virus</i< (SMV). As efficient detection technology is crucial for the field management of SMV, novel immunological detection methods were developed in the present study. According to the phylogenetic analysis, the CP coding sequence of SMV-SC7 was selected for the prokaryotic expression of the recombinant SMV-CP. Purified SMV-CP was used for the development of polyclonal antibodies (PAb) against the SMV-CP (PAb-SMV-CP) and monoclonal antibodies (MAb) against SMV-CP (MAb-SMV-CP). Subsequently, the PAb-SMV-CP was used for the development of a novel DAS- quantitative ELISA (DAS-qELISA) kit, of which the sensitivity was greater than 1:4000, and this could be used for the quantitative detection of SMV in China. Meanwhile, the MAb-SMV-CP was labeled with colloidal gold, and then was used for the development of the SMV-specific gold immunochromatography strip (SMV-GICS). The SMV-GICS gives accurate detection results through observed control lines and test lines in 5 to 10 min, sharing the same sensitivity as RT-PCR, and can be used for rapid, accurate and high-throughput field SMV detection. The DAS-qELISA kit and the SMV-GICA strip developed in this study are SMV-specific, sensitive, cheap and easy to use. These products will be conducive to the timely, efficient SMV epidemiology and detection in major soybean-producing regions in China and abroad. <i<Soybean mosaic virus</i< SMV-CP PAb-SMV-CP MAb-SMV-CP DAS-qELISA SMV-GICS Biology (General) Chemistry Tao Wang verfasserin aut Le Gao verfasserin aut Puwen Song verfasserin aut Yunhua Yang verfasserin aut Haijian Zhi verfasserin aut Kai Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 16, p 9457 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:16, p 9457 https://doi.org/10.3390/ijms23169457 kostenfrei https://doaj.org/article/edab347900e94511b12c86a7f616443c kostenfrei https://www.mdpi.com/1422-0067/23/16/9457 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 16, p 9457
language	English
source	In International Journal of Molecular Sciences 23(2022), 16, p 9457 volume:23 year:2022 number:16, p 9457
sourceStr	In International Journal of Molecular Sciences 23(2022), 16, p 9457 volume:23 year:2022 number:16, p 9457
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Soybean mosaic virus</i< SMV-CP PAb-SMV-CP MAb-SMV-CP DAS-qELISA SMV-GICS Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Rui Ren @@aut@@ Tao Wang @@aut@@ Le Gao @@aut@@ Puwen Song @@aut@@ Yunhua Yang @@aut@@ Haijian Zhi @@aut@@ Kai Li @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ030312671
language_de	englisch
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callnumber-first	Q - Science
author	Rui Ren
spellingShingle	Rui Ren misc QH301-705.5 misc QD1-999 misc <i<Soybean mosaic virus</i< misc SMV-CP misc PAb-SMV-CP misc MAb-SMV-CP misc DAS-qELISA misc SMV-GICS misc Biology (General) misc Chemistry Development of Comprehensive Serological Techniques for Sensitive, Quantitative and Rapid Detection of <i<Soybean mosaic virus</i<
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topic_title	QH301-705.5 QD1-999 Development of Comprehensive Serological Techniques for Sensitive, Quantitative and Rapid Detection of <i<Soybean mosaic virus</i< <i<Soybean mosaic virus</i< SMV-CP PAb-SMV-CP MAb-SMV-CP DAS-qELISA SMV-GICS
topic	misc QH301-705.5 misc QD1-999 misc <i<Soybean mosaic virus</i< misc SMV-CP misc PAb-SMV-CP misc MAb-SMV-CP misc DAS-qELISA misc SMV-GICS misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc <i<Soybean mosaic virus</i< misc SMV-CP misc PAb-SMV-CP misc MAb-SMV-CP misc DAS-qELISA misc SMV-GICS misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc <i<Soybean mosaic virus</i< misc SMV-CP misc PAb-SMV-CP misc MAb-SMV-CP misc DAS-qELISA misc SMV-GICS misc Biology (General) misc Chemistry
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hierarchy_parent_title	International Journal of Molecular Sciences
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title	Development of Comprehensive Serological Techniques for Sensitive, Quantitative and Rapid Detection of <i<Soybean mosaic virus</i<
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title_full	Development of Comprehensive Serological Techniques for Sensitive, Quantitative and Rapid Detection of <i<Soybean mosaic virus</i<
author_sort	Rui Ren
journal	International Journal of Molecular Sciences
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author_browse	Rui Ren Tao Wang Le Gao Puwen Song Yunhua Yang Haijian Zhi Kai Li
container_volume	23
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author-letter	Rui Ren
doi_str_mv	10.3390/ijms23169457
author2-role	verfasserin
title_sort	development of comprehensive serological techniques for sensitive, quantitative and rapid detection of <i<soybean mosaic virus</i<
callnumber	QH301-705.5
title_auth	Development of Comprehensive Serological Techniques for Sensitive, Quantitative and Rapid Detection of <i<Soybean mosaic virus</i<
abstract	Soybean is an important grain and oil crop worldwide; however, the yield and seed quality of which are seriously affected by <i<Soybean mosaic virus</i< (SMV). As efficient detection technology is crucial for the field management of SMV, novel immunological detection methods were developed in the present study. According to the phylogenetic analysis, the CP coding sequence of SMV-SC7 was selected for the prokaryotic expression of the recombinant SMV-CP. Purified SMV-CP was used for the development of polyclonal antibodies (PAb) against the SMV-CP (PAb-SMV-CP) and monoclonal antibodies (MAb) against SMV-CP (MAb-SMV-CP). Subsequently, the PAb-SMV-CP was used for the development of a novel DAS- quantitative ELISA (DAS-qELISA) kit, of which the sensitivity was greater than 1:4000, and this could be used for the quantitative detection of SMV in China. Meanwhile, the MAb-SMV-CP was labeled with colloidal gold, and then was used for the development of the SMV-specific gold immunochromatography strip (SMV-GICS). The SMV-GICS gives accurate detection results through observed control lines and test lines in 5 to 10 min, sharing the same sensitivity as RT-PCR, and can be used for rapid, accurate and high-throughput field SMV detection. The DAS-qELISA kit and the SMV-GICA strip developed in this study are SMV-specific, sensitive, cheap and easy to use. These products will be conducive to the timely, efficient SMV epidemiology and detection in major soybean-producing regions in China and abroad.
abstractGer	Soybean is an important grain and oil crop worldwide; however, the yield and seed quality of which are seriously affected by <i<Soybean mosaic virus</i< (SMV). As efficient detection technology is crucial for the field management of SMV, novel immunological detection methods were developed in the present study. According to the phylogenetic analysis, the CP coding sequence of SMV-SC7 was selected for the prokaryotic expression of the recombinant SMV-CP. Purified SMV-CP was used for the development of polyclonal antibodies (PAb) against the SMV-CP (PAb-SMV-CP) and monoclonal antibodies (MAb) against SMV-CP (MAb-SMV-CP). Subsequently, the PAb-SMV-CP was used for the development of a novel DAS- quantitative ELISA (DAS-qELISA) kit, of which the sensitivity was greater than 1:4000, and this could be used for the quantitative detection of SMV in China. Meanwhile, the MAb-SMV-CP was labeled with colloidal gold, and then was used for the development of the SMV-specific gold immunochromatography strip (SMV-GICS). The SMV-GICS gives accurate detection results through observed control lines and test lines in 5 to 10 min, sharing the same sensitivity as RT-PCR, and can be used for rapid, accurate and high-throughput field SMV detection. The DAS-qELISA kit and the SMV-GICA strip developed in this study are SMV-specific, sensitive, cheap and easy to use. These products will be conducive to the timely, efficient SMV epidemiology and detection in major soybean-producing regions in China and abroad.
abstract_unstemmed	Soybean is an important grain and oil crop worldwide; however, the yield and seed quality of which are seriously affected by <i<Soybean mosaic virus</i< (SMV). As efficient detection technology is crucial for the field management of SMV, novel immunological detection methods were developed in the present study. According to the phylogenetic analysis, the CP coding sequence of SMV-SC7 was selected for the prokaryotic expression of the recombinant SMV-CP. Purified SMV-CP was used for the development of polyclonal antibodies (PAb) against the SMV-CP (PAb-SMV-CP) and monoclonal antibodies (MAb) against SMV-CP (MAb-SMV-CP). Subsequently, the PAb-SMV-CP was used for the development of a novel DAS- quantitative ELISA (DAS-qELISA) kit, of which the sensitivity was greater than 1:4000, and this could be used for the quantitative detection of SMV in China. Meanwhile, the MAb-SMV-CP was labeled with colloidal gold, and then was used for the development of the SMV-specific gold immunochromatography strip (SMV-GICS). The SMV-GICS gives accurate detection results through observed control lines and test lines in 5 to 10 min, sharing the same sensitivity as RT-PCR, and can be used for rapid, accurate and high-throughput field SMV detection. The DAS-qELISA kit and the SMV-GICA strip developed in this study are SMV-specific, sensitive, cheap and easy to use. These products will be conducive to the timely, efficient SMV epidemiology and detection in major soybean-producing regions in China and abroad.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	16, p 9457
title_short	Development of Comprehensive Serological Techniques for Sensitive, Quantitative and Rapid Detection of <i<Soybean mosaic virus</i<
url	https://doi.org/10.3390/ijms23169457 https://doaj.org/article/edab347900e94511b12c86a7f616443c https://www.mdpi.com/1422-0067/23/16/9457 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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up_date	2024-07-03T14:14:33.597Z
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