Sanguinarine Induces H<sub<2</sub<O<sub<2</sub<-Dependent Apoptosis and Ferroptosis in Human Cervical Cancer

Sanguinarine (SNG) is a benzophenanthridine alkaloid isolated mainly from <i<Sanguinaria canadensis</i<, <i<Chelidonium majus</i<, and <i<Macleaya cordata</i<. SNG is considered an antineoplastic agent based on its cytotoxic activity against various tumors. Howeve...
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Gespeichert in:

Autor*in:	Ameer Alakkal [verfasserIn] Faisal Thayyullathil [verfasserIn] Siraj Pallichankandy [verfasserIn] Karthikeyan Subburayan [verfasserIn] Anees Rahman Cheratta [verfasserIn] Sehamuddin Galadari [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	apoptosis ferroptosis LPO labile iron ROS sanguinarine

Übergeordnetes Werk:	In: Biomedicines - MDPI AG, 2014, 10(2022), 8, p 1795
Übergeordnetes Werk:	volume:10 ; year:2022 ; number:8, p 1795

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/biomedicines10081795

Katalog-ID:	DOAJ030360811

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520			\|a Sanguinarine (SNG) is a benzophenanthridine alkaloid isolated mainly from <i<Sanguinaria canadensis</i<, <i<Chelidonium majus</i<, and <i<Macleaya cordata</i<. SNG is considered an antineoplastic agent based on its cytotoxic activity against various tumors. However, the exact molecular mechanism through which SNG mediates this activity has not been elucidated. Here, we report that SNG induces death in human cervical cancer (HeLa) cells through activation of two interdependent cell death pathways—apoptosis and ferroptosis. SNG-induced apoptosis was characterized by caspase activation and PARP cleavage, while ferroptosis involved solute carrier family 7 member 11 (SLC7A11) down-regulation, glutathione (GSH) depletion, iron accumulation, and lipid peroxidation (LPO). Interestingly, incubation with caspase inhibitor z-VAD-fmk not only inhibited the features of apoptosis, but also negated markers of SNG-induced ferroptosis. Similarly, pretreatment with ferroptosis inhibitor ferrostatin-1 (Fer-1), apart from rescuing cells from SNG-induced ferroptosis, also curbed the features of SNG-induced apoptosis. Our study implies that, together, apoptosis and ferroptosis act as partners in the context of SNG mediated tumor suppression in HeLa cells. Importantly, SNG increased the generation of reactive oxygen species (ROS), and ROS inhibition blocks the induction of both apoptosis and ferroptosis. These findings highlight the value of continued investigation into the potential use of SNG as an antineoplastic agent.
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spelling	10.3390/biomedicines10081795 doi (DE-627)DOAJ030360811 (DE-599)DOAJ1ee47b4a477e4f4a9ad27dc0e2a2d018 DE-627 ger DE-627 rakwb eng QH301-705.5 Ameer Alakkal verfasserin aut Sanguinarine Induces H<sub<2</sub<O<sub<2</sub<-Dependent Apoptosis and Ferroptosis in Human Cervical Cancer 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Sanguinarine (SNG) is a benzophenanthridine alkaloid isolated mainly from <i<Sanguinaria canadensis</i<, <i<Chelidonium majus</i<, and <i<Macleaya cordata</i<. SNG is considered an antineoplastic agent based on its cytotoxic activity against various tumors. However, the exact molecular mechanism through which SNG mediates this activity has not been elucidated. Here, we report that SNG induces death in human cervical cancer (HeLa) cells through activation of two interdependent cell death pathways—apoptosis and ferroptosis. SNG-induced apoptosis was characterized by caspase activation and PARP cleavage, while ferroptosis involved solute carrier family 7 member 11 (SLC7A11) down-regulation, glutathione (GSH) depletion, iron accumulation, and lipid peroxidation (LPO). Interestingly, incubation with caspase inhibitor z-VAD-fmk not only inhibited the features of apoptosis, but also negated markers of SNG-induced ferroptosis. Similarly, pretreatment with ferroptosis inhibitor ferrostatin-1 (Fer-1), apart from rescuing cells from SNG-induced ferroptosis, also curbed the features of SNG-induced apoptosis. Our study implies that, together, apoptosis and ferroptosis act as partners in the context of SNG mediated tumor suppression in HeLa cells. Importantly, SNG increased the generation of reactive oxygen species (ROS), and ROS inhibition blocks the induction of both apoptosis and ferroptosis. These findings highlight the value of continued investigation into the potential use of SNG as an antineoplastic agent. apoptosis ferroptosis LPO labile iron ROS sanguinarine Biology (General) Faisal Thayyullathil verfasserin aut Siraj Pallichankandy verfasserin aut Karthikeyan Subburayan verfasserin aut Anees Rahman Cheratta verfasserin aut Sehamuddin Galadari verfasserin aut In Biomedicines MDPI AG, 2014 10(2022), 8, p 1795 (DE-627)750370483 (DE-600)2720867-9 22279059 nnns volume:10 year:2022 number:8, p 1795 https://doi.org/10.3390/biomedicines10081795 kostenfrei https://doaj.org/article/1ee47b4a477e4f4a9ad27dc0e2a2d018 kostenfrei https://www.mdpi.com/2227-9059/10/8/1795 kostenfrei https://doaj.org/toc/2227-9059 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2022 8, p 1795
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allfieldsSound	10.3390/biomedicines10081795 doi (DE-627)DOAJ030360811 (DE-599)DOAJ1ee47b4a477e4f4a9ad27dc0e2a2d018 DE-627 ger DE-627 rakwb eng QH301-705.5 Ameer Alakkal verfasserin aut Sanguinarine Induces H<sub<2</sub<O<sub<2</sub<-Dependent Apoptosis and Ferroptosis in Human Cervical Cancer 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Sanguinarine (SNG) is a benzophenanthridine alkaloid isolated mainly from <i<Sanguinaria canadensis</i<, <i<Chelidonium majus</i<, and <i<Macleaya cordata</i<. SNG is considered an antineoplastic agent based on its cytotoxic activity against various tumors. However, the exact molecular mechanism through which SNG mediates this activity has not been elucidated. Here, we report that SNG induces death in human cervical cancer (HeLa) cells through activation of two interdependent cell death pathways—apoptosis and ferroptosis. SNG-induced apoptosis was characterized by caspase activation and PARP cleavage, while ferroptosis involved solute carrier family 7 member 11 (SLC7A11) down-regulation, glutathione (GSH) depletion, iron accumulation, and lipid peroxidation (LPO). Interestingly, incubation with caspase inhibitor z-VAD-fmk not only inhibited the features of apoptosis, but also negated markers of SNG-induced ferroptosis. Similarly, pretreatment with ferroptosis inhibitor ferrostatin-1 (Fer-1), apart from rescuing cells from SNG-induced ferroptosis, also curbed the features of SNG-induced apoptosis. Our study implies that, together, apoptosis and ferroptosis act as partners in the context of SNG mediated tumor suppression in HeLa cells. Importantly, SNG increased the generation of reactive oxygen species (ROS), and ROS inhibition blocks the induction of both apoptosis and ferroptosis. These findings highlight the value of continued investigation into the potential use of SNG as an antineoplastic agent. apoptosis ferroptosis LPO labile iron ROS sanguinarine Biology (General) Faisal Thayyullathil verfasserin aut Siraj Pallichankandy verfasserin aut Karthikeyan Subburayan verfasserin aut Anees Rahman Cheratta verfasserin aut Sehamuddin Galadari verfasserin aut In Biomedicines MDPI AG, 2014 10(2022), 8, p 1795 (DE-627)750370483 (DE-600)2720867-9 22279059 nnns volume:10 year:2022 number:8, p 1795 https://doi.org/10.3390/biomedicines10081795 kostenfrei https://doaj.org/article/1ee47b4a477e4f4a9ad27dc0e2a2d018 kostenfrei https://www.mdpi.com/2227-9059/10/8/1795 kostenfrei https://doaj.org/toc/2227-9059 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2022 8, p 1795
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author	Ameer Alakkal
spellingShingle	Ameer Alakkal misc QH301-705.5 misc apoptosis misc ferroptosis misc LPO misc labile iron misc ROS misc sanguinarine misc Biology (General) Sanguinarine Induces H<sub<2</sub<O<sub<2</sub<-Dependent Apoptosis and Ferroptosis in Human Cervical Cancer
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topic_title	QH301-705.5 Sanguinarine Induces H<sub<2</sub<O<sub<2</sub<-Dependent Apoptosis and Ferroptosis in Human Cervical Cancer apoptosis ferroptosis LPO labile iron ROS sanguinarine
topic	misc QH301-705.5 misc apoptosis misc ferroptosis misc LPO misc labile iron misc ROS misc sanguinarine misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc apoptosis misc ferroptosis misc LPO misc labile iron misc ROS misc sanguinarine misc Biology (General)
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title	Sanguinarine Induces H<sub<2</sub<O<sub<2</sub<-Dependent Apoptosis and Ferroptosis in Human Cervical Cancer
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title_full	Sanguinarine Induces H<sub<2</sub<O<sub<2</sub<-Dependent Apoptosis and Ferroptosis in Human Cervical Cancer
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title_sort	sanguinarine induces h<sub<2</sub<o<sub<2</sub<-dependent apoptosis and ferroptosis in human cervical cancer
callnumber	QH301-705.5
title_auth	Sanguinarine Induces H<sub<2</sub<O<sub<2</sub<-Dependent Apoptosis and Ferroptosis in Human Cervical Cancer
abstract	Sanguinarine (SNG) is a benzophenanthridine alkaloid isolated mainly from <i<Sanguinaria canadensis</i<, <i<Chelidonium majus</i<, and <i<Macleaya cordata</i<. SNG is considered an antineoplastic agent based on its cytotoxic activity against various tumors. However, the exact molecular mechanism through which SNG mediates this activity has not been elucidated. Here, we report that SNG induces death in human cervical cancer (HeLa) cells through activation of two interdependent cell death pathways—apoptosis and ferroptosis. SNG-induced apoptosis was characterized by caspase activation and PARP cleavage, while ferroptosis involved solute carrier family 7 member 11 (SLC7A11) down-regulation, glutathione (GSH) depletion, iron accumulation, and lipid peroxidation (LPO). Interestingly, incubation with caspase inhibitor z-VAD-fmk not only inhibited the features of apoptosis, but also negated markers of SNG-induced ferroptosis. Similarly, pretreatment with ferroptosis inhibitor ferrostatin-1 (Fer-1), apart from rescuing cells from SNG-induced ferroptosis, also curbed the features of SNG-induced apoptosis. Our study implies that, together, apoptosis and ferroptosis act as partners in the context of SNG mediated tumor suppression in HeLa cells. Importantly, SNG increased the generation of reactive oxygen species (ROS), and ROS inhibition blocks the induction of both apoptosis and ferroptosis. These findings highlight the value of continued investigation into the potential use of SNG as an antineoplastic agent.
abstractGer	Sanguinarine (SNG) is a benzophenanthridine alkaloid isolated mainly from <i<Sanguinaria canadensis</i<, <i<Chelidonium majus</i<, and <i<Macleaya cordata</i<. SNG is considered an antineoplastic agent based on its cytotoxic activity against various tumors. However, the exact molecular mechanism through which SNG mediates this activity has not been elucidated. Here, we report that SNG induces death in human cervical cancer (HeLa) cells through activation of two interdependent cell death pathways—apoptosis and ferroptosis. SNG-induced apoptosis was characterized by caspase activation and PARP cleavage, while ferroptosis involved solute carrier family 7 member 11 (SLC7A11) down-regulation, glutathione (GSH) depletion, iron accumulation, and lipid peroxidation (LPO). Interestingly, incubation with caspase inhibitor z-VAD-fmk not only inhibited the features of apoptosis, but also negated markers of SNG-induced ferroptosis. Similarly, pretreatment with ferroptosis inhibitor ferrostatin-1 (Fer-1), apart from rescuing cells from SNG-induced ferroptosis, also curbed the features of SNG-induced apoptosis. Our study implies that, together, apoptosis and ferroptosis act as partners in the context of SNG mediated tumor suppression in HeLa cells. Importantly, SNG increased the generation of reactive oxygen species (ROS), and ROS inhibition blocks the induction of both apoptosis and ferroptosis. These findings highlight the value of continued investigation into the potential use of SNG as an antineoplastic agent.
abstract_unstemmed	Sanguinarine (SNG) is a benzophenanthridine alkaloid isolated mainly from <i<Sanguinaria canadensis</i<, <i<Chelidonium majus</i<, and <i<Macleaya cordata</i<. SNG is considered an antineoplastic agent based on its cytotoxic activity against various tumors. However, the exact molecular mechanism through which SNG mediates this activity has not been elucidated. Here, we report that SNG induces death in human cervical cancer (HeLa) cells through activation of two interdependent cell death pathways—apoptosis and ferroptosis. SNG-induced apoptosis was characterized by caspase activation and PARP cleavage, while ferroptosis involved solute carrier family 7 member 11 (SLC7A11) down-regulation, glutathione (GSH) depletion, iron accumulation, and lipid peroxidation (LPO). Interestingly, incubation with caspase inhibitor z-VAD-fmk not only inhibited the features of apoptosis, but also negated markers of SNG-induced ferroptosis. Similarly, pretreatment with ferroptosis inhibitor ferrostatin-1 (Fer-1), apart from rescuing cells from SNG-induced ferroptosis, also curbed the features of SNG-induced apoptosis. Our study implies that, together, apoptosis and ferroptosis act as partners in the context of SNG mediated tumor suppression in HeLa cells. Importantly, SNG increased the generation of reactive oxygen species (ROS), and ROS inhibition blocks the induction of both apoptosis and ferroptosis. These findings highlight the value of continued investigation into the potential use of SNG as an antineoplastic agent.
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title_short	Sanguinarine Induces H<sub<2</sub<O<sub<2</sub<-Dependent Apoptosis and Ferroptosis in Human Cervical Cancer
url	https://doi.org/10.3390/biomedicines10081795 https://doaj.org/article/1ee47b4a477e4f4a9ad27dc0e2a2d018 https://www.mdpi.com/2227-9059/10/8/1795 https://doaj.org/toc/2227-9059
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up_date	2024-07-03T14:32:48.390Z
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Our study implies that, together, apoptosis and ferroptosis act as partners in the context of SNG mediated tumor suppression in HeLa cells. Importantly, SNG increased the generation of reactive oxygen species (ROS), and ROS inhibition blocks the induction of both apoptosis and ferroptosis. 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Sanguinarine Induces H<sub<2</sub<O<sub<2</sub<-Dependent Apoptosis and Ferroptosis in Human Cervical Cancer

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