Autofluorescence Detection Method for Dental Plaque Bacteria Detection and Classification: Example of <i<Porphyromonas gingivalis</i<, <i<Aggregatibacter actinomycetemcomitans</i<, and <i<Streptococcus mutans</i<

The use of fluorescence spectroscopy for plaque detection is a fast and effective way to monitor oral health. At present, there is no uniform specification for the design of the excitation light source of related products for generating fluorescence. To carry out experiments on dental plaque, the fl...
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Gespeichert in:

Autor*in:	Yung-Jhe Yan [verfasserIn] Bo-Wen Wang [verfasserIn] Chih-Man Yang [verfasserIn] Ching-Yi Wu [verfasserIn] Mang Ou-Yang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	plaque detection dental plaque autofluorescence

Übergeordnetes Werk:	In: Dentistry Journal - MDPI AG, 2013, 9(2021), 7, p 74
Übergeordnetes Werk:	volume:9 ; year:2021 ; number:7, p 74

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/dj9070074

Katalog-ID:	DOAJ030407850

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callnumber-first	R - Medicine
author	Yung-Jhe Yan
spellingShingle	Yung-Jhe Yan misc RK1-715 misc plaque detection misc dental plaque misc autofluorescence misc <i<Porphyromonas gingivalis</i< misc <i<Aggregatibacter actinomycetemcomitans</i< misc <i<Streptococcus mutans</i< misc Dentistry Autofluorescence Detection Method for Dental Plaque Bacteria Detection and Classification: Example of <i<Porphyromonas gingivalis</i<, <i<Aggregatibacter actinomycetemcomitans</i<, and <i<Streptococcus mutans</i<
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topic_title	RK1-715 Autofluorescence Detection Method for Dental Plaque Bacteria Detection and Classification: Example of <i<Porphyromonas gingivalis</i<, <i<Aggregatibacter actinomycetemcomitans</i<, and <i<Streptococcus mutans</i< plaque detection dental plaque autofluorescence <i<Porphyromonas gingivalis</i< <i<Aggregatibacter actinomycetemcomitans</i< <i<Streptococcus mutans</i<
topic	misc RK1-715 misc plaque detection misc dental plaque misc autofluorescence misc <i<Porphyromonas gingivalis</i< misc <i<Aggregatibacter actinomycetemcomitans</i< misc <i<Streptococcus mutans</i< misc Dentistry
topic_unstemmed	misc RK1-715 misc plaque detection misc dental plaque misc autofluorescence misc <i<Porphyromonas gingivalis</i< misc <i<Aggregatibacter actinomycetemcomitans</i< misc <i<Streptococcus mutans</i< misc Dentistry
topic_browse	misc RK1-715 misc plaque detection misc dental plaque misc autofluorescence misc <i<Porphyromonas gingivalis</i< misc <i<Aggregatibacter actinomycetemcomitans</i< misc <i<Streptococcus mutans</i< misc Dentistry
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title	Autofluorescence Detection Method for Dental Plaque Bacteria Detection and Classification: Example of <i<Porphyromonas gingivalis</i<, <i<Aggregatibacter actinomycetemcomitans</i<, and <i<Streptococcus mutans</i<
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title_full	Autofluorescence Detection Method for Dental Plaque Bacteria Detection and Classification: Example of <i<Porphyromonas gingivalis</i<, <i<Aggregatibacter actinomycetemcomitans</i<, and <i<Streptococcus mutans</i<
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title_sort	autofluorescence detection method for dental plaque bacteria detection and classification: example of <i<porphyromonas gingivalis</i<, <i<aggregatibacter actinomycetemcomitans</i<, and <i<streptococcus mutans</i<
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title_auth	Autofluorescence Detection Method for Dental Plaque Bacteria Detection and Classification: Example of <i<Porphyromonas gingivalis</i<, <i<Aggregatibacter actinomycetemcomitans</i<, and <i<Streptococcus mutans</i<
abstract	The use of fluorescence spectroscopy for plaque detection is a fast and effective way to monitor oral health. At present, there is no uniform specification for the design of the excitation light source of related products for generating fluorescence. To carry out experiments on dental plaque, the fluorescence spectra of three different bacterial species (<i<Porphyromonas gingivalis</i<, <i<Aggregatibacter actinomycetemcomitans</i<, and <i<Streptococcus mutans</i<) were measured by hyperspectral imaging microscopy (HIM). Three critical issues were found in the experiments. One issue was the unwanted spectrum generated from a mercury line source; two four-order low-pass filters were evaluated for eliminating the unwanted spectrum and meet the experimental requirements. The second issue was the red fluorescence generated from the microscope slide made of borosilicate glass; this could affect the observation of the red fluorescence from the bacteria; quartz microscope slides were found to reduce the fluorescence intensity by about 2 dB compared with the borosilicate slide. The third issue of photobleaching in the fluorescence of the <i<Porphyromonas gingivalis</i< was studied. This study proposes a method of classifying three bacteria based on the spectral intensity ratios (510/635 and 500/635 nm) under the 405 nm excitation light was proposed in this study. The sensitivity and specificity of the classification were approximately 99% and 99%, respectively.
abstractGer	The use of fluorescence spectroscopy for plaque detection is a fast and effective way to monitor oral health. At present, there is no uniform specification for the design of the excitation light source of related products for generating fluorescence. To carry out experiments on dental plaque, the fluorescence spectra of three different bacterial species (<i<Porphyromonas gingivalis</i<, <i<Aggregatibacter actinomycetemcomitans</i<, and <i<Streptococcus mutans</i<) were measured by hyperspectral imaging microscopy (HIM). Three critical issues were found in the experiments. One issue was the unwanted spectrum generated from a mercury line source; two four-order low-pass filters were evaluated for eliminating the unwanted spectrum and meet the experimental requirements. The second issue was the red fluorescence generated from the microscope slide made of borosilicate glass; this could affect the observation of the red fluorescence from the bacteria; quartz microscope slides were found to reduce the fluorescence intensity by about 2 dB compared with the borosilicate slide. The third issue of photobleaching in the fluorescence of the <i<Porphyromonas gingivalis</i< was studied. This study proposes a method of classifying three bacteria based on the spectral intensity ratios (510/635 and 500/635 nm) under the 405 nm excitation light was proposed in this study. The sensitivity and specificity of the classification were approximately 99% and 99%, respectively.
abstract_unstemmed	The use of fluorescence spectroscopy for plaque detection is a fast and effective way to monitor oral health. At present, there is no uniform specification for the design of the excitation light source of related products for generating fluorescence. To carry out experiments on dental plaque, the fluorescence spectra of three different bacterial species (<i<Porphyromonas gingivalis</i<, <i<Aggregatibacter actinomycetemcomitans</i<, and <i<Streptococcus mutans</i<) were measured by hyperspectral imaging microscopy (HIM). Three critical issues were found in the experiments. One issue was the unwanted spectrum generated from a mercury line source; two four-order low-pass filters were evaluated for eliminating the unwanted spectrum and meet the experimental requirements. The second issue was the red fluorescence generated from the microscope slide made of borosilicate glass; this could affect the observation of the red fluorescence from the bacteria; quartz microscope slides were found to reduce the fluorescence intensity by about 2 dB compared with the borosilicate slide. The third issue of photobleaching in the fluorescence of the <i<Porphyromonas gingivalis</i< was studied. This study proposes a method of classifying three bacteria based on the spectral intensity ratios (510/635 and 500/635 nm) under the 405 nm excitation light was proposed in this study. The sensitivity and specificity of the classification were approximately 99% and 99%, respectively.
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container_issue	7, p 74
title_short	Autofluorescence Detection Method for Dental Plaque Bacteria Detection and Classification: Example of <i<Porphyromonas gingivalis</i<, <i<Aggregatibacter actinomycetemcomitans</i<, and <i<Streptococcus mutans</i<
url	https://doi.org/10.3390/dj9070074 https://doaj.org/article/7ab008f4bc74455bb971d2408639acee https://www.mdpi.com/2304-6767/9/7/74 https://doaj.org/toc/2304-6767
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up_date	2024-07-03T14:50:07.238Z
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The third issue of photobleaching in the fluorescence of the <i<Porphyromonas gingivalis</i< was studied. This study proposes a method of classifying three bacteria based on the spectral intensity ratios (510/635 and 500/635 nm) under the 405 nm excitation light was proposed in this study. 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Autofluorescence Detection Method for Dental Plaque Bacteria Detection and Classification: Example of <i<Porphyromonas gingivalis</i<, <i<Aggregatibacter actinomycetemcomitans</i<, and <i<Streptococcus mutans</i<

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