A Comparative Study on Delivery of Externally Attached DNA by Papillomavirus VLPs and Pseudoviruses

Human papillomavirus (HPV) 16 capsids have been chosen as a DNA delivery vehicle in many studies. Our preliminary studies suggest that HPV58 capsids could be better vehicles than HPV16 capsids to deliver encapsidated DNA in vitro and in vivo. In the current study, we compared HPV16, HPV58, and the c...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Sarah Brendle [verfasserIn] Nancy Cladel [verfasserIn] Karla Balogh [verfasserIn] Samina Alam [verfasserIn] Neil Christensen [verfasserIn] Craig Meyers [verfasserIn] Jiafen Hu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	virus-like particles (VLPs) L1 L2 pseudovirus (PSV) papillomavirus DNA delivery

Übergeordnetes Werk:	In: Vaccines - MDPI AG, 2013, 9(2021), 12, p 1501
Übergeordnetes Werk:	volume:9 ; year:2021 ; number:12, p 1501

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/vaccines9121501

Katalog-ID:	DOAJ03064092X

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allfields	10.3390/vaccines9121501 doi (DE-627)DOAJ03064092X (DE-599)DOAJa7ab6ba1f3484d4088f43c4381a6e2f6 DE-627 ger DE-627 rakwb eng Sarah Brendle verfasserin aut A Comparative Study on Delivery of Externally Attached DNA by Papillomavirus VLPs and Pseudoviruses 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Human papillomavirus (HPV) 16 capsids have been chosen as a DNA delivery vehicle in many studies. Our preliminary studies suggest that HPV58 capsids could be better vehicles than HPV16 capsids to deliver encapsidated DNA in vitro and in vivo. In the current study, we compared HPV16, HPV58, and the cottontail rabbit papillomavirus (CRPV) capsids either as L1/L2 VLPs or pseudoviruses (PSVs) to deliver externally attached GFP-expressing DNA. Both rabbit and human cells were used to test whether there was a species-specific effect. DNA delivery efficiency was determined by quantifying either GFP-expressing cell populations or mean fluorescent intensities (MFI) by flow cytometry. Interestingly, CRPV and 58-VLPs and PSVs were significantly more efficient at delivering attached DNA when compared to 16-VLPs and PSVs. A capsid/DNA ratio of 2:1 showed the highest efficiency for delivering external DNA. The PSVs with papillomavirus DNA genomes also showed higher efficiency than those with irrelevant plasmid DNA. HPV16L1/58L2 hybrid VLPs displayed increased efficiency compared to HPV58L1/16L2 VLPs, suggesting that L2 may play a critical role in the delivery of attached DNA. Additionally, we demonstrated that VLPs increased in vivo infectivity of CRPV DNA in rabbits. We conclude that choosing CRPV or 58 capsids to deliver external DNA could improve DNA uptake in in vitro and in vivo models. virus-like particles (VLPs) L1 L2 pseudovirus (PSV) papillomavirus DNA delivery Medicine R Nancy Cladel verfasserin aut Karla Balogh verfasserin aut Samina Alam verfasserin aut Neil Christensen verfasserin aut Craig Meyers verfasserin aut Jiafen Hu verfasserin aut In Vaccines MDPI AG, 2013 9(2021), 12, p 1501 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:9 year:2021 number:12, p 1501 https://doi.org/10.3390/vaccines9121501 kostenfrei https://doaj.org/article/a7ab6ba1f3484d4088f43c4381a6e2f6 kostenfrei https://www.mdpi.com/2076-393X/9/12/1501 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 12, p 1501
spelling	10.3390/vaccines9121501 doi (DE-627)DOAJ03064092X (DE-599)DOAJa7ab6ba1f3484d4088f43c4381a6e2f6 DE-627 ger DE-627 rakwb eng Sarah Brendle verfasserin aut A Comparative Study on Delivery of Externally Attached DNA by Papillomavirus VLPs and Pseudoviruses 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Human papillomavirus (HPV) 16 capsids have been chosen as a DNA delivery vehicle in many studies. Our preliminary studies suggest that HPV58 capsids could be better vehicles than HPV16 capsids to deliver encapsidated DNA in vitro and in vivo. In the current study, we compared HPV16, HPV58, and the cottontail rabbit papillomavirus (CRPV) capsids either as L1/L2 VLPs or pseudoviruses (PSVs) to deliver externally attached GFP-expressing DNA. Both rabbit and human cells were used to test whether there was a species-specific effect. DNA delivery efficiency was determined by quantifying either GFP-expressing cell populations or mean fluorescent intensities (MFI) by flow cytometry. Interestingly, CRPV and 58-VLPs and PSVs were significantly more efficient at delivering attached DNA when compared to 16-VLPs and PSVs. A capsid/DNA ratio of 2:1 showed the highest efficiency for delivering external DNA. The PSVs with papillomavirus DNA genomes also showed higher efficiency than those with irrelevant plasmid DNA. HPV16L1/58L2 hybrid VLPs displayed increased efficiency compared to HPV58L1/16L2 VLPs, suggesting that L2 may play a critical role in the delivery of attached DNA. Additionally, we demonstrated that VLPs increased in vivo infectivity of CRPV DNA in rabbits. We conclude that choosing CRPV or 58 capsids to deliver external DNA could improve DNA uptake in in vitro and in vivo models. virus-like particles (VLPs) L1 L2 pseudovirus (PSV) papillomavirus DNA delivery Medicine R Nancy Cladel verfasserin aut Karla Balogh verfasserin aut Samina Alam verfasserin aut Neil Christensen verfasserin aut Craig Meyers verfasserin aut Jiafen Hu verfasserin aut In Vaccines MDPI AG, 2013 9(2021), 12, p 1501 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:9 year:2021 number:12, p 1501 https://doi.org/10.3390/vaccines9121501 kostenfrei https://doaj.org/article/a7ab6ba1f3484d4088f43c4381a6e2f6 kostenfrei https://www.mdpi.com/2076-393X/9/12/1501 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 12, p 1501
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allfieldsGer	10.3390/vaccines9121501 doi (DE-627)DOAJ03064092X (DE-599)DOAJa7ab6ba1f3484d4088f43c4381a6e2f6 DE-627 ger DE-627 rakwb eng Sarah Brendle verfasserin aut A Comparative Study on Delivery of Externally Attached DNA by Papillomavirus VLPs and Pseudoviruses 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Human papillomavirus (HPV) 16 capsids have been chosen as a DNA delivery vehicle in many studies. Our preliminary studies suggest that HPV58 capsids could be better vehicles than HPV16 capsids to deliver encapsidated DNA in vitro and in vivo. In the current study, we compared HPV16, HPV58, and the cottontail rabbit papillomavirus (CRPV) capsids either as L1/L2 VLPs or pseudoviruses (PSVs) to deliver externally attached GFP-expressing DNA. Both rabbit and human cells were used to test whether there was a species-specific effect. DNA delivery efficiency was determined by quantifying either GFP-expressing cell populations or mean fluorescent intensities (MFI) by flow cytometry. Interestingly, CRPV and 58-VLPs and PSVs were significantly more efficient at delivering attached DNA when compared to 16-VLPs and PSVs. A capsid/DNA ratio of 2:1 showed the highest efficiency for delivering external DNA. The PSVs with papillomavirus DNA genomes also showed higher efficiency than those with irrelevant plasmid DNA. HPV16L1/58L2 hybrid VLPs displayed increased efficiency compared to HPV58L1/16L2 VLPs, suggesting that L2 may play a critical role in the delivery of attached DNA. Additionally, we demonstrated that VLPs increased in vivo infectivity of CRPV DNA in rabbits. We conclude that choosing CRPV or 58 capsids to deliver external DNA could improve DNA uptake in in vitro and in vivo models. virus-like particles (VLPs) L1 L2 pseudovirus (PSV) papillomavirus DNA delivery Medicine R Nancy Cladel verfasserin aut Karla Balogh verfasserin aut Samina Alam verfasserin aut Neil Christensen verfasserin aut Craig Meyers verfasserin aut Jiafen Hu verfasserin aut In Vaccines MDPI AG, 2013 9(2021), 12, p 1501 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:9 year:2021 number:12, p 1501 https://doi.org/10.3390/vaccines9121501 kostenfrei https://doaj.org/article/a7ab6ba1f3484d4088f43c4381a6e2f6 kostenfrei https://www.mdpi.com/2076-393X/9/12/1501 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 12, p 1501
allfieldsSound	10.3390/vaccines9121501 doi (DE-627)DOAJ03064092X (DE-599)DOAJa7ab6ba1f3484d4088f43c4381a6e2f6 DE-627 ger DE-627 rakwb eng Sarah Brendle verfasserin aut A Comparative Study on Delivery of Externally Attached DNA by Papillomavirus VLPs and Pseudoviruses 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Human papillomavirus (HPV) 16 capsids have been chosen as a DNA delivery vehicle in many studies. Our preliminary studies suggest that HPV58 capsids could be better vehicles than HPV16 capsids to deliver encapsidated DNA in vitro and in vivo. In the current study, we compared HPV16, HPV58, and the cottontail rabbit papillomavirus (CRPV) capsids either as L1/L2 VLPs or pseudoviruses (PSVs) to deliver externally attached GFP-expressing DNA. Both rabbit and human cells were used to test whether there was a species-specific effect. DNA delivery efficiency was determined by quantifying either GFP-expressing cell populations or mean fluorescent intensities (MFI) by flow cytometry. Interestingly, CRPV and 58-VLPs and PSVs were significantly more efficient at delivering attached DNA when compared to 16-VLPs and PSVs. A capsid/DNA ratio of 2:1 showed the highest efficiency for delivering external DNA. The PSVs with papillomavirus DNA genomes also showed higher efficiency than those with irrelevant plasmid DNA. HPV16L1/58L2 hybrid VLPs displayed increased efficiency compared to HPV58L1/16L2 VLPs, suggesting that L2 may play a critical role in the delivery of attached DNA. Additionally, we demonstrated that VLPs increased in vivo infectivity of CRPV DNA in rabbits. We conclude that choosing CRPV or 58 capsids to deliver external DNA could improve DNA uptake in in vitro and in vivo models. virus-like particles (VLPs) L1 L2 pseudovirus (PSV) papillomavirus DNA delivery Medicine R Nancy Cladel verfasserin aut Karla Balogh verfasserin aut Samina Alam verfasserin aut Neil Christensen verfasserin aut Craig Meyers verfasserin aut Jiafen Hu verfasserin aut In Vaccines MDPI AG, 2013 9(2021), 12, p 1501 (DE-627)736559205 (DE-600)2703319-3 2076393X nnns volume:9 year:2021 number:12, p 1501 https://doi.org/10.3390/vaccines9121501 kostenfrei https://doaj.org/article/a7ab6ba1f3484d4088f43c4381a6e2f6 kostenfrei https://www.mdpi.com/2076-393X/9/12/1501 kostenfrei https://doaj.org/toc/2076-393X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 12, p 1501
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source	In Vaccines 9(2021), 12, p 1501 volume:9 year:2021 number:12, p 1501
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authorswithroles_txt_mv	Sarah Brendle @@aut@@ Nancy Cladel @@aut@@ Karla Balogh @@aut@@ Samina Alam @@aut@@ Neil Christensen @@aut@@ Craig Meyers @@aut@@ Jiafen Hu @@aut@@
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author	Sarah Brendle
spellingShingle	Sarah Brendle misc virus-like particles (VLPs) misc L1 misc L2 misc pseudovirus (PSV) misc papillomavirus misc DNA delivery misc Medicine misc R A Comparative Study on Delivery of Externally Attached DNA by Papillomavirus VLPs and Pseudoviruses
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topic_title	A Comparative Study on Delivery of Externally Attached DNA by Papillomavirus VLPs and Pseudoviruses virus-like particles (VLPs) L1 L2 pseudovirus (PSV) papillomavirus DNA delivery
topic	misc virus-like particles (VLPs) misc L1 misc L2 misc pseudovirus (PSV) misc papillomavirus misc DNA delivery misc Medicine misc R
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title	A Comparative Study on Delivery of Externally Attached DNA by Papillomavirus VLPs and Pseudoviruses
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title_full	A Comparative Study on Delivery of Externally Attached DNA by Papillomavirus VLPs and Pseudoviruses
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author_browse	Sarah Brendle Nancy Cladel Karla Balogh Samina Alam Neil Christensen Craig Meyers Jiafen Hu
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title_sort	comparative study on delivery of externally attached dna by papillomavirus vlps and pseudoviruses
title_auth	A Comparative Study on Delivery of Externally Attached DNA by Papillomavirus VLPs and Pseudoviruses
abstract	Human papillomavirus (HPV) 16 capsids have been chosen as a DNA delivery vehicle in many studies. Our preliminary studies suggest that HPV58 capsids could be better vehicles than HPV16 capsids to deliver encapsidated DNA in vitro and in vivo. In the current study, we compared HPV16, HPV58, and the cottontail rabbit papillomavirus (CRPV) capsids either as L1/L2 VLPs or pseudoviruses (PSVs) to deliver externally attached GFP-expressing DNA. Both rabbit and human cells were used to test whether there was a species-specific effect. DNA delivery efficiency was determined by quantifying either GFP-expressing cell populations or mean fluorescent intensities (MFI) by flow cytometry. Interestingly, CRPV and 58-VLPs and PSVs were significantly more efficient at delivering attached DNA when compared to 16-VLPs and PSVs. A capsid/DNA ratio of 2:1 showed the highest efficiency for delivering external DNA. The PSVs with papillomavirus DNA genomes also showed higher efficiency than those with irrelevant plasmid DNA. HPV16L1/58L2 hybrid VLPs displayed increased efficiency compared to HPV58L1/16L2 VLPs, suggesting that L2 may play a critical role in the delivery of attached DNA. Additionally, we demonstrated that VLPs increased in vivo infectivity of CRPV DNA in rabbits. We conclude that choosing CRPV or 58 capsids to deliver external DNA could improve DNA uptake in in vitro and in vivo models.
abstractGer	Human papillomavirus (HPV) 16 capsids have been chosen as a DNA delivery vehicle in many studies. Our preliminary studies suggest that HPV58 capsids could be better vehicles than HPV16 capsids to deliver encapsidated DNA in vitro and in vivo. In the current study, we compared HPV16, HPV58, and the cottontail rabbit papillomavirus (CRPV) capsids either as L1/L2 VLPs or pseudoviruses (PSVs) to deliver externally attached GFP-expressing DNA. Both rabbit and human cells were used to test whether there was a species-specific effect. DNA delivery efficiency was determined by quantifying either GFP-expressing cell populations or mean fluorescent intensities (MFI) by flow cytometry. Interestingly, CRPV and 58-VLPs and PSVs were significantly more efficient at delivering attached DNA when compared to 16-VLPs and PSVs. A capsid/DNA ratio of 2:1 showed the highest efficiency for delivering external DNA. The PSVs with papillomavirus DNA genomes also showed higher efficiency than those with irrelevant plasmid DNA. HPV16L1/58L2 hybrid VLPs displayed increased efficiency compared to HPV58L1/16L2 VLPs, suggesting that L2 may play a critical role in the delivery of attached DNA. Additionally, we demonstrated that VLPs increased in vivo infectivity of CRPV DNA in rabbits. We conclude that choosing CRPV or 58 capsids to deliver external DNA could improve DNA uptake in in vitro and in vivo models.
abstract_unstemmed	Human papillomavirus (HPV) 16 capsids have been chosen as a DNA delivery vehicle in many studies. Our preliminary studies suggest that HPV58 capsids could be better vehicles than HPV16 capsids to deliver encapsidated DNA in vitro and in vivo. In the current study, we compared HPV16, HPV58, and the cottontail rabbit papillomavirus (CRPV) capsids either as L1/L2 VLPs or pseudoviruses (PSVs) to deliver externally attached GFP-expressing DNA. Both rabbit and human cells were used to test whether there was a species-specific effect. DNA delivery efficiency was determined by quantifying either GFP-expressing cell populations or mean fluorescent intensities (MFI) by flow cytometry. Interestingly, CRPV and 58-VLPs and PSVs were significantly more efficient at delivering attached DNA when compared to 16-VLPs and PSVs. A capsid/DNA ratio of 2:1 showed the highest efficiency for delivering external DNA. The PSVs with papillomavirus DNA genomes also showed higher efficiency than those with irrelevant plasmid DNA. HPV16L1/58L2 hybrid VLPs displayed increased efficiency compared to HPV58L1/16L2 VLPs, suggesting that L2 may play a critical role in the delivery of attached DNA. Additionally, we demonstrated that VLPs increased in vivo infectivity of CRPV DNA in rabbits. We conclude that choosing CRPV or 58 capsids to deliver external DNA could improve DNA uptake in in vitro and in vivo models.
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title_short	A Comparative Study on Delivery of Externally Attached DNA by Papillomavirus VLPs and Pseudoviruses
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author2	Nancy Cladel Karla Balogh Samina Alam Neil Christensen Craig Meyers Jiafen Hu
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A Comparative Study on Delivery of Externally Attached DNA by Papillomavirus VLPs and Pseudoviruses

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