Identification and Expression Analysis of Chemosensory Genes in the Antennal Transcriptome of Chrysanthemum Aphid <i<Macrosiphoniella sanborni</i<

As one of the most destructive oligophagous pests, the chrysanthemum aphid (<i<Macrosiphoniella sanborni</i<) has seriously restricted the sustainable development of the chrysanthemum industry. Olfaction plays a critical role in the environmental perception of aphids, but very little is...
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Autor*in:	Jian Zhong [verfasserIn] Yuxin Wang [verfasserIn] Yufan Lu [verfasserIn] Xiaoou Ma [verfasserIn] Qian Zhang [verfasserIn] Xiaoyue Wang [verfasserIn] Qixiang Zhang [verfasserIn] Ming Sun [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	antennal transcriptome chemosensory genes aphid

Übergeordnetes Werk:	In: Insects - MDPI AG, 2011, 13(2022), 7, p 597
Übergeordnetes Werk:	volume:13 ; year:2022 ; number:7, p 597

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/insects13070597

Katalog-ID:	DOAJ030901618

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520			\|a As one of the most destructive oligophagous pests, the chrysanthemum aphid (<i<Macrosiphoniella sanborni</i<) has seriously restricted the sustainable development of the chrysanthemum industry. Olfaction plays a critical role in the environmental perception of aphids, but very little is currently known about the chemosensory mechanism of <i<M. sanborni</i<. In this study, four MsanOBPs, four MsanCSPs, eight MsanORs, two MsanIRs and one MsanSNMP were identified among the 28,323 unigenes derived from the antennal transcriptome bioinformatic analysis of <i<M. sanborni</i< adults. Then, comprehensive phylogenetic analyses of these olfactory-related proteins in different aphid species were performed using multiple sequence alignment. Subsequently, the odor-specific and wing-specific expression profiles of these candidate chemosensory genes were investigated using quantitative real-time PCR. The data showed that most of these chemosensory genes exhibited higher expression levels in alate aphids. Among them, <i<MsanOBP9</i<, <i<MsanOR2</i<, <i<MsanOR4</i<, <i<MsanOR43b-1</i<, <i<MsanCSP1</i<, <i<MsanCSP2</i<, <i<MsanCSP4</i<, <i<MsanIR25a</i< and <i<MsanIR40a</i< in alate aphids showed remarkably higher expression levels than in apterous aphids under the effect of the host plant volatiles, indicating that these genes may take part in the specific behaviors of alate adults, such as host recognition, oviposition site selection and so on. This study lays the groundwork for future research into the molecular mechanism of olfactory recognition in <i<M. sanborni</i<.
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allfields	10.3390/insects13070597 doi (DE-627)DOAJ030901618 (DE-599)DOAJc30f8f0889004b15b9983cc33e7a6103 DE-627 ger DE-627 rakwb eng Jian Zhong verfasserin aut Identification and Expression Analysis of Chemosensory Genes in the Antennal Transcriptome of Chrysanthemum Aphid <i<Macrosiphoniella sanborni</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As one of the most destructive oligophagous pests, the chrysanthemum aphid (<i<Macrosiphoniella sanborni</i<) has seriously restricted the sustainable development of the chrysanthemum industry. Olfaction plays a critical role in the environmental perception of aphids, but very little is currently known about the chemosensory mechanism of <i<M. sanborni</i<. In this study, four MsanOBPs, four MsanCSPs, eight MsanORs, two MsanIRs and one MsanSNMP were identified among the 28,323 unigenes derived from the antennal transcriptome bioinformatic analysis of <i<M. sanborni</i< adults. Then, comprehensive phylogenetic analyses of these olfactory-related proteins in different aphid species were performed using multiple sequence alignment. Subsequently, the odor-specific and wing-specific expression profiles of these candidate chemosensory genes were investigated using quantitative real-time PCR. The data showed that most of these chemosensory genes exhibited higher expression levels in alate aphids. Among them, <i<MsanOBP9</i<, <i<MsanOR2</i<, <i<MsanOR4</i<, <i<MsanOR43b-1</i<, <i<MsanCSP1</i<, <i<MsanCSP2</i<, <i<MsanCSP4</i<, <i<MsanIR25a</i< and <i<MsanIR40a</i< in alate aphids showed remarkably higher expression levels than in apterous aphids under the effect of the host plant volatiles, indicating that these genes may take part in the specific behaviors of alate adults, such as host recognition, oviposition site selection and so on. This study lays the groundwork for future research into the molecular mechanism of olfactory recognition in <i<M. sanborni</i<. <i<Macrosiphoniella sanborni</i< antennal transcriptome chemosensory genes aphid Science Q Yuxin Wang verfasserin aut Yufan Lu verfasserin aut Xiaoou Ma verfasserin aut Qian Zhang verfasserin aut Xiaoyue Wang verfasserin aut Qixiang Zhang verfasserin aut Ming Sun verfasserin aut In Insects MDPI AG, 2011 13(2022), 7, p 597 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:13 year:2022 number:7, p 597 https://doi.org/10.3390/insects13070597 kostenfrei https://doaj.org/article/c30f8f0889004b15b9983cc33e7a6103 kostenfrei https://www.mdpi.com/2075-4450/13/7/597 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 7, p 597
spelling	10.3390/insects13070597 doi (DE-627)DOAJ030901618 (DE-599)DOAJc30f8f0889004b15b9983cc33e7a6103 DE-627 ger DE-627 rakwb eng Jian Zhong verfasserin aut Identification and Expression Analysis of Chemosensory Genes in the Antennal Transcriptome of Chrysanthemum Aphid <i<Macrosiphoniella sanborni</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As one of the most destructive oligophagous pests, the chrysanthemum aphid (<i<Macrosiphoniella sanborni</i<) has seriously restricted the sustainable development of the chrysanthemum industry. Olfaction plays a critical role in the environmental perception of aphids, but very little is currently known about the chemosensory mechanism of <i<M. sanborni</i<. In this study, four MsanOBPs, four MsanCSPs, eight MsanORs, two MsanIRs and one MsanSNMP were identified among the 28,323 unigenes derived from the antennal transcriptome bioinformatic analysis of <i<M. sanborni</i< adults. Then, comprehensive phylogenetic analyses of these olfactory-related proteins in different aphid species were performed using multiple sequence alignment. Subsequently, the odor-specific and wing-specific expression profiles of these candidate chemosensory genes were investigated using quantitative real-time PCR. The data showed that most of these chemosensory genes exhibited higher expression levels in alate aphids. Among them, <i<MsanOBP9</i<, <i<MsanOR2</i<, <i<MsanOR4</i<, <i<MsanOR43b-1</i<, <i<MsanCSP1</i<, <i<MsanCSP2</i<, <i<MsanCSP4</i<, <i<MsanIR25a</i< and <i<MsanIR40a</i< in alate aphids showed remarkably higher expression levels than in apterous aphids under the effect of the host plant volatiles, indicating that these genes may take part in the specific behaviors of alate adults, such as host recognition, oviposition site selection and so on. This study lays the groundwork for future research into the molecular mechanism of olfactory recognition in <i<M. sanborni</i<. <i<Macrosiphoniella sanborni</i< antennal transcriptome chemosensory genes aphid Science Q Yuxin Wang verfasserin aut Yufan Lu verfasserin aut Xiaoou Ma verfasserin aut Qian Zhang verfasserin aut Xiaoyue Wang verfasserin aut Qixiang Zhang verfasserin aut Ming Sun verfasserin aut In Insects MDPI AG, 2011 13(2022), 7, p 597 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:13 year:2022 number:7, p 597 https://doi.org/10.3390/insects13070597 kostenfrei https://doaj.org/article/c30f8f0889004b15b9983cc33e7a6103 kostenfrei https://www.mdpi.com/2075-4450/13/7/597 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 7, p 597
allfields_unstemmed	10.3390/insects13070597 doi (DE-627)DOAJ030901618 (DE-599)DOAJc30f8f0889004b15b9983cc33e7a6103 DE-627 ger DE-627 rakwb eng Jian Zhong verfasserin aut Identification and Expression Analysis of Chemosensory Genes in the Antennal Transcriptome of Chrysanthemum Aphid <i<Macrosiphoniella sanborni</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As one of the most destructive oligophagous pests, the chrysanthemum aphid (<i<Macrosiphoniella sanborni</i<) has seriously restricted the sustainable development of the chrysanthemum industry. Olfaction plays a critical role in the environmental perception of aphids, but very little is currently known about the chemosensory mechanism of <i<M. sanborni</i<. In this study, four MsanOBPs, four MsanCSPs, eight MsanORs, two MsanIRs and one MsanSNMP were identified among the 28,323 unigenes derived from the antennal transcriptome bioinformatic analysis of <i<M. sanborni</i< adults. Then, comprehensive phylogenetic analyses of these olfactory-related proteins in different aphid species were performed using multiple sequence alignment. Subsequently, the odor-specific and wing-specific expression profiles of these candidate chemosensory genes were investigated using quantitative real-time PCR. The data showed that most of these chemosensory genes exhibited higher expression levels in alate aphids. Among them, <i<MsanOBP9</i<, <i<MsanOR2</i<, <i<MsanOR4</i<, <i<MsanOR43b-1</i<, <i<MsanCSP1</i<, <i<MsanCSP2</i<, <i<MsanCSP4</i<, <i<MsanIR25a</i< and <i<MsanIR40a</i< in alate aphids showed remarkably higher expression levels than in apterous aphids under the effect of the host plant volatiles, indicating that these genes may take part in the specific behaviors of alate adults, such as host recognition, oviposition site selection and so on. This study lays the groundwork for future research into the molecular mechanism of olfactory recognition in <i<M. sanborni</i<. <i<Macrosiphoniella sanborni</i< antennal transcriptome chemosensory genes aphid Science Q Yuxin Wang verfasserin aut Yufan Lu verfasserin aut Xiaoou Ma verfasserin aut Qian Zhang verfasserin aut Xiaoyue Wang verfasserin aut Qixiang Zhang verfasserin aut Ming Sun verfasserin aut In Insects MDPI AG, 2011 13(2022), 7, p 597 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:13 year:2022 number:7, p 597 https://doi.org/10.3390/insects13070597 kostenfrei https://doaj.org/article/c30f8f0889004b15b9983cc33e7a6103 kostenfrei https://www.mdpi.com/2075-4450/13/7/597 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 7, p 597
allfieldsGer	10.3390/insects13070597 doi (DE-627)DOAJ030901618 (DE-599)DOAJc30f8f0889004b15b9983cc33e7a6103 DE-627 ger DE-627 rakwb eng Jian Zhong verfasserin aut Identification and Expression Analysis of Chemosensory Genes in the Antennal Transcriptome of Chrysanthemum Aphid <i<Macrosiphoniella sanborni</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As one of the most destructive oligophagous pests, the chrysanthemum aphid (<i<Macrosiphoniella sanborni</i<) has seriously restricted the sustainable development of the chrysanthemum industry. Olfaction plays a critical role in the environmental perception of aphids, but very little is currently known about the chemosensory mechanism of <i<M. sanborni</i<. In this study, four MsanOBPs, four MsanCSPs, eight MsanORs, two MsanIRs and one MsanSNMP were identified among the 28,323 unigenes derived from the antennal transcriptome bioinformatic analysis of <i<M. sanborni</i< adults. Then, comprehensive phylogenetic analyses of these olfactory-related proteins in different aphid species were performed using multiple sequence alignment. Subsequently, the odor-specific and wing-specific expression profiles of these candidate chemosensory genes were investigated using quantitative real-time PCR. The data showed that most of these chemosensory genes exhibited higher expression levels in alate aphids. Among them, <i<MsanOBP9</i<, <i<MsanOR2</i<, <i<MsanOR4</i<, <i<MsanOR43b-1</i<, <i<MsanCSP1</i<, <i<MsanCSP2</i<, <i<MsanCSP4</i<, <i<MsanIR25a</i< and <i<MsanIR40a</i< in alate aphids showed remarkably higher expression levels than in apterous aphids under the effect of the host plant volatiles, indicating that these genes may take part in the specific behaviors of alate adults, such as host recognition, oviposition site selection and so on. This study lays the groundwork for future research into the molecular mechanism of olfactory recognition in <i<M. sanborni</i<. <i<Macrosiphoniella sanborni</i< antennal transcriptome chemosensory genes aphid Science Q Yuxin Wang verfasserin aut Yufan Lu verfasserin aut Xiaoou Ma verfasserin aut Qian Zhang verfasserin aut Xiaoyue Wang verfasserin aut Qixiang Zhang verfasserin aut Ming Sun verfasserin aut In Insects MDPI AG, 2011 13(2022), 7, p 597 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:13 year:2022 number:7, p 597 https://doi.org/10.3390/insects13070597 kostenfrei https://doaj.org/article/c30f8f0889004b15b9983cc33e7a6103 kostenfrei https://www.mdpi.com/2075-4450/13/7/597 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 7, p 597
allfieldsSound	10.3390/insects13070597 doi (DE-627)DOAJ030901618 (DE-599)DOAJc30f8f0889004b15b9983cc33e7a6103 DE-627 ger DE-627 rakwb eng Jian Zhong verfasserin aut Identification and Expression Analysis of Chemosensory Genes in the Antennal Transcriptome of Chrysanthemum Aphid <i<Macrosiphoniella sanborni</i< 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As one of the most destructive oligophagous pests, the chrysanthemum aphid (<i<Macrosiphoniella sanborni</i<) has seriously restricted the sustainable development of the chrysanthemum industry. Olfaction plays a critical role in the environmental perception of aphids, but very little is currently known about the chemosensory mechanism of <i<M. sanborni</i<. In this study, four MsanOBPs, four MsanCSPs, eight MsanORs, two MsanIRs and one MsanSNMP were identified among the 28,323 unigenes derived from the antennal transcriptome bioinformatic analysis of <i<M. sanborni</i< adults. Then, comprehensive phylogenetic analyses of these olfactory-related proteins in different aphid species were performed using multiple sequence alignment. Subsequently, the odor-specific and wing-specific expression profiles of these candidate chemosensory genes were investigated using quantitative real-time PCR. The data showed that most of these chemosensory genes exhibited higher expression levels in alate aphids. Among them, <i<MsanOBP9</i<, <i<MsanOR2</i<, <i<MsanOR4</i<, <i<MsanOR43b-1</i<, <i<MsanCSP1</i<, <i<MsanCSP2</i<, <i<MsanCSP4</i<, <i<MsanIR25a</i< and <i<MsanIR40a</i< in alate aphids showed remarkably higher expression levels than in apterous aphids under the effect of the host plant volatiles, indicating that these genes may take part in the specific behaviors of alate adults, such as host recognition, oviposition site selection and so on. This study lays the groundwork for future research into the molecular mechanism of olfactory recognition in <i<M. sanborni</i<. <i<Macrosiphoniella sanborni</i< antennal transcriptome chemosensory genes aphid Science Q Yuxin Wang verfasserin aut Yufan Lu verfasserin aut Xiaoou Ma verfasserin aut Qian Zhang verfasserin aut Xiaoyue Wang verfasserin aut Qixiang Zhang verfasserin aut Ming Sun verfasserin aut In Insects MDPI AG, 2011 13(2022), 7, p 597 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:13 year:2022 number:7, p 597 https://doi.org/10.3390/insects13070597 kostenfrei https://doaj.org/article/c30f8f0889004b15b9983cc33e7a6103 kostenfrei https://www.mdpi.com/2075-4450/13/7/597 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 7, p 597
language	English
source	In Insects 13(2022), 7, p 597 volume:13 year:2022 number:7, p 597
sourceStr	In Insects 13(2022), 7, p 597 volume:13 year:2022 number:7, p 597
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Macrosiphoniella sanborni</i< antennal transcriptome chemosensory genes aphid Science Q
isfreeaccess_bool	true
container_title	Insects
authorswithroles_txt_mv	Jian Zhong @@aut@@ Yuxin Wang @@aut@@ Yufan Lu @@aut@@ Xiaoou Ma @@aut@@ Qian Zhang @@aut@@ Xiaoyue Wang @@aut@@ Qixiang Zhang @@aut@@ Ming Sun @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	718627121
id	DOAJ030901618
language_de	englisch
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author	Jian Zhong
spellingShingle	Jian Zhong misc <i<Macrosiphoniella sanborni</i< misc antennal transcriptome misc chemosensory genes misc aphid misc Science misc Q Identification and Expression Analysis of Chemosensory Genes in the Antennal Transcriptome of Chrysanthemum Aphid <i<Macrosiphoniella sanborni</i<
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topic_title	Identification and Expression Analysis of Chemosensory Genes in the Antennal Transcriptome of Chrysanthemum Aphid <i<Macrosiphoniella sanborni</i< <i<Macrosiphoniella sanborni</i< antennal transcriptome chemosensory genes aphid
topic	misc <i<Macrosiphoniella sanborni</i< misc antennal transcriptome misc chemosensory genes misc aphid misc Science misc Q
topic_unstemmed	misc <i<Macrosiphoniella sanborni</i< misc antennal transcriptome misc chemosensory genes misc aphid misc Science misc Q
topic_browse	misc <i<Macrosiphoniella sanborni</i< misc antennal transcriptome misc chemosensory genes misc aphid misc Science misc Q
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title	Identification and Expression Analysis of Chemosensory Genes in the Antennal Transcriptome of Chrysanthemum Aphid <i<Macrosiphoniella sanborni</i<
ctrlnum	(DE-627)DOAJ030901618 (DE-599)DOAJc30f8f0889004b15b9983cc33e7a6103
title_full	Identification and Expression Analysis of Chemosensory Genes in the Antennal Transcriptome of Chrysanthemum Aphid <i<Macrosiphoniella sanborni</i<
author_sort	Jian Zhong
journal	Insects
journalStr	Insects
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author_browse	Jian Zhong Yuxin Wang Yufan Lu Xiaoou Ma Qian Zhang Xiaoyue Wang Qixiang Zhang Ming Sun
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author-letter	Jian Zhong
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title_sort	identification and expression analysis of chemosensory genes in the antennal transcriptome of chrysanthemum aphid <i<macrosiphoniella sanborni</i<
title_auth	Identification and Expression Analysis of Chemosensory Genes in the Antennal Transcriptome of Chrysanthemum Aphid <i<Macrosiphoniella sanborni</i<
abstract	As one of the most destructive oligophagous pests, the chrysanthemum aphid (<i<Macrosiphoniella sanborni</i<) has seriously restricted the sustainable development of the chrysanthemum industry. Olfaction plays a critical role in the environmental perception of aphids, but very little is currently known about the chemosensory mechanism of <i<M. sanborni</i<. In this study, four MsanOBPs, four MsanCSPs, eight MsanORs, two MsanIRs and one MsanSNMP were identified among the 28,323 unigenes derived from the antennal transcriptome bioinformatic analysis of <i<M. sanborni</i< adults. Then, comprehensive phylogenetic analyses of these olfactory-related proteins in different aphid species were performed using multiple sequence alignment. Subsequently, the odor-specific and wing-specific expression profiles of these candidate chemosensory genes were investigated using quantitative real-time PCR. The data showed that most of these chemosensory genes exhibited higher expression levels in alate aphids. Among them, <i<MsanOBP9</i<, <i<MsanOR2</i<, <i<MsanOR4</i<, <i<MsanOR43b-1</i<, <i<MsanCSP1</i<, <i<MsanCSP2</i<, <i<MsanCSP4</i<, <i<MsanIR25a</i< and <i<MsanIR40a</i< in alate aphids showed remarkably higher expression levels than in apterous aphids under the effect of the host plant volatiles, indicating that these genes may take part in the specific behaviors of alate adults, such as host recognition, oviposition site selection and so on. This study lays the groundwork for future research into the molecular mechanism of olfactory recognition in <i<M. sanborni</i<.
abstractGer	As one of the most destructive oligophagous pests, the chrysanthemum aphid (<i<Macrosiphoniella sanborni</i<) has seriously restricted the sustainable development of the chrysanthemum industry. Olfaction plays a critical role in the environmental perception of aphids, but very little is currently known about the chemosensory mechanism of <i<M. sanborni</i<. In this study, four MsanOBPs, four MsanCSPs, eight MsanORs, two MsanIRs and one MsanSNMP were identified among the 28,323 unigenes derived from the antennal transcriptome bioinformatic analysis of <i<M. sanborni</i< adults. Then, comprehensive phylogenetic analyses of these olfactory-related proteins in different aphid species were performed using multiple sequence alignment. Subsequently, the odor-specific and wing-specific expression profiles of these candidate chemosensory genes were investigated using quantitative real-time PCR. The data showed that most of these chemosensory genes exhibited higher expression levels in alate aphids. Among them, <i<MsanOBP9</i<, <i<MsanOR2</i<, <i<MsanOR4</i<, <i<MsanOR43b-1</i<, <i<MsanCSP1</i<, <i<MsanCSP2</i<, <i<MsanCSP4</i<, <i<MsanIR25a</i< and <i<MsanIR40a</i< in alate aphids showed remarkably higher expression levels than in apterous aphids under the effect of the host plant volatiles, indicating that these genes may take part in the specific behaviors of alate adults, such as host recognition, oviposition site selection and so on. This study lays the groundwork for future research into the molecular mechanism of olfactory recognition in <i<M. sanborni</i<.
abstract_unstemmed	As one of the most destructive oligophagous pests, the chrysanthemum aphid (<i<Macrosiphoniella sanborni</i<) has seriously restricted the sustainable development of the chrysanthemum industry. Olfaction plays a critical role in the environmental perception of aphids, but very little is currently known about the chemosensory mechanism of <i<M. sanborni</i<. In this study, four MsanOBPs, four MsanCSPs, eight MsanORs, two MsanIRs and one MsanSNMP were identified among the 28,323 unigenes derived from the antennal transcriptome bioinformatic analysis of <i<M. sanborni</i< adults. Then, comprehensive phylogenetic analyses of these olfactory-related proteins in different aphid species were performed using multiple sequence alignment. Subsequently, the odor-specific and wing-specific expression profiles of these candidate chemosensory genes were investigated using quantitative real-time PCR. The data showed that most of these chemosensory genes exhibited higher expression levels in alate aphids. Among them, <i<MsanOBP9</i<, <i<MsanOR2</i<, <i<MsanOR4</i<, <i<MsanOR43b-1</i<, <i<MsanCSP1</i<, <i<MsanCSP2</i<, <i<MsanCSP4</i<, <i<MsanIR25a</i< and <i<MsanIR40a</i< in alate aphids showed remarkably higher expression levels than in apterous aphids under the effect of the host plant volatiles, indicating that these genes may take part in the specific behaviors of alate adults, such as host recognition, oviposition site selection and so on. This study lays the groundwork for future research into the molecular mechanism of olfactory recognition in <i<M. sanborni</i<.
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container_issue	7, p 597
title_short	Identification and Expression Analysis of Chemosensory Genes in the Antennal Transcriptome of Chrysanthemum Aphid <i<Macrosiphoniella sanborni</i<
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Identification and Expression Analysis of Chemosensory Genes in the Antennal Transcriptome of Chrysanthemum Aphid <i<Macrosiphoniella sanborni</i<

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