Comparison of Humoral and Cellular CMV Immunity in Patients Awaiting Kidney Transplantation

Chronic kidney disease may alter antiviral T cell immunity. In the current study, we assessed in 63 patients prior to kidney transplantation how humoral and cellular immunity against cytomegalovirus (CMV) correlated using an interferon (IFN)-γ ELISpot (T-Track<sup<®</sup< CMV, Mikrogen,...
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Autor*in:	Monika Lindemann [verfasserIn] Benjamin Wilde [verfasserIn] Justa Friebus-Kardash [verfasserIn] Anja Gäckler [verfasserIn] Oliver Witzke [verfasserIn] Ulf Dittmer [verfasserIn] Peter A. Horn [verfasserIn] Andreas Kribben [verfasserIn] Nils Mülling [verfasserIn] Ute Eisenberger [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	human cytomegalovirus ELISpot interferon-γ dialysis immunosuppressive therapy

Übergeordnetes Werk:	In: Diagnostics - MDPI AG, 2012, 11(2021), 9, p 1688
Übergeordnetes Werk:	volume:11 ; year:2021 ; number:9, p 1688

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/diagnostics11091688

Katalog-ID:	DOAJ031014828

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520			\|a Chronic kidney disease may alter antiviral T cell immunity. In the current study, we assessed in 63 patients prior to kidney transplantation how humoral and cellular immunity against cytomegalovirus (CMV) correlated using an interferon (IFN)-γ ELISpot (T-Track<sup<®</sup< CMV, Mikrogen, Neuried, Germany). The cohort comprised 24 patients with negative and 39 with positive CMV IgG. Whereas none of the patients with negative CMV IgG showed detectable responses to the T-Track<sup<®</sup< CMV, 26 out of 39 patients with positive CMV IgG had positive ELISpot responses. The median response to CMV pp65 in the CMV seronegative group was 0 spot forming units (SFU) per 200,000 PBMC (range 0–1) and in the seropositive group 43 SFU (range 0–750). Thus, 13 out of 39 patients with positive CMV serostatus (33%) had undetectable T cell immunity and may be at an increased risk of CMV reactivation. CMV pp65-specific ELISpot responses were 29.3-fold higher in seropositive patients with vs. without dialysis and 5.6-fold higher in patients with vs. without immunosuppressive therapy, but patients with dialysis and immunosuppressive therapy showed, as expected, lower responses to phytohemagglutinin, the positive control. This finding may be caused by (subclinical) CMV-DNAemia and a “booster” of CMV-specific T cells.
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allfields	10.3390/diagnostics11091688 doi (DE-627)DOAJ031014828 (DE-599)DOAJcd1a2c7b6520438abf000e49a5c3aea9 DE-627 ger DE-627 rakwb eng R5-920 Monika Lindemann verfasserin aut Comparison of Humoral and Cellular CMV Immunity in Patients Awaiting Kidney Transplantation 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chronic kidney disease may alter antiviral T cell immunity. In the current study, we assessed in 63 patients prior to kidney transplantation how humoral and cellular immunity against cytomegalovirus (CMV) correlated using an interferon (IFN)-γ ELISpot (T-Track<sup<®</sup< CMV, Mikrogen, Neuried, Germany). The cohort comprised 24 patients with negative and 39 with positive CMV IgG. Whereas none of the patients with negative CMV IgG showed detectable responses to the T-Track<sup<®</sup< CMV, 26 out of 39 patients with positive CMV IgG had positive ELISpot responses. The median response to CMV pp65 in the CMV seronegative group was 0 spot forming units (SFU) per 200,000 PBMC (range 0–1) and in the seropositive group 43 SFU (range 0–750). Thus, 13 out of 39 patients with positive CMV serostatus (33%) had undetectable T cell immunity and may be at an increased risk of CMV reactivation. CMV pp65-specific ELISpot responses were 29.3-fold higher in seropositive patients with vs. without dialysis and 5.6-fold higher in patients with vs. without immunosuppressive therapy, but patients with dialysis and immunosuppressive therapy showed, as expected, lower responses to phytohemagglutinin, the positive control. This finding may be caused by (subclinical) CMV-DNAemia and a “booster” of CMV-specific T cells. human cytomegalovirus ELISpot interferon-γ dialysis immunosuppressive therapy Medicine (General) Benjamin Wilde verfasserin aut Justa Friebus-Kardash verfasserin aut Anja Gäckler verfasserin aut Oliver Witzke verfasserin aut Ulf Dittmer verfasserin aut Peter A. Horn verfasserin aut Andreas Kribben verfasserin aut Nils Mülling verfasserin aut Ute Eisenberger verfasserin aut In Diagnostics MDPI AG, 2012 11(2021), 9, p 1688 (DE-627)718627814 (DE-600)2662336-5 20754418 nnns volume:11 year:2021 number:9, p 1688 https://doi.org/10.3390/diagnostics11091688 kostenfrei https://doaj.org/article/cd1a2c7b6520438abf000e49a5c3aea9 kostenfrei https://www.mdpi.com/2075-4418/11/9/1688 kostenfrei https://doaj.org/toc/2075-4418 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 9, p 1688
spelling	10.3390/diagnostics11091688 doi (DE-627)DOAJ031014828 (DE-599)DOAJcd1a2c7b6520438abf000e49a5c3aea9 DE-627 ger DE-627 rakwb eng R5-920 Monika Lindemann verfasserin aut Comparison of Humoral and Cellular CMV Immunity in Patients Awaiting Kidney Transplantation 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chronic kidney disease may alter antiviral T cell immunity. In the current study, we assessed in 63 patients prior to kidney transplantation how humoral and cellular immunity against cytomegalovirus (CMV) correlated using an interferon (IFN)-γ ELISpot (T-Track<sup<®</sup< CMV, Mikrogen, Neuried, Germany). The cohort comprised 24 patients with negative and 39 with positive CMV IgG. Whereas none of the patients with negative CMV IgG showed detectable responses to the T-Track<sup<®</sup< CMV, 26 out of 39 patients with positive CMV IgG had positive ELISpot responses. The median response to CMV pp65 in the CMV seronegative group was 0 spot forming units (SFU) per 200,000 PBMC (range 0–1) and in the seropositive group 43 SFU (range 0–750). Thus, 13 out of 39 patients with positive CMV serostatus (33%) had undetectable T cell immunity and may be at an increased risk of CMV reactivation. CMV pp65-specific ELISpot responses were 29.3-fold higher in seropositive patients with vs. without dialysis and 5.6-fold higher in patients with vs. without immunosuppressive therapy, but patients with dialysis and immunosuppressive therapy showed, as expected, lower responses to phytohemagglutinin, the positive control. This finding may be caused by (subclinical) CMV-DNAemia and a “booster” of CMV-specific T cells. human cytomegalovirus ELISpot interferon-γ dialysis immunosuppressive therapy Medicine (General) Benjamin Wilde verfasserin aut Justa Friebus-Kardash verfasserin aut Anja Gäckler verfasserin aut Oliver Witzke verfasserin aut Ulf Dittmer verfasserin aut Peter A. Horn verfasserin aut Andreas Kribben verfasserin aut Nils Mülling verfasserin aut Ute Eisenberger verfasserin aut In Diagnostics MDPI AG, 2012 11(2021), 9, p 1688 (DE-627)718627814 (DE-600)2662336-5 20754418 nnns volume:11 year:2021 number:9, p 1688 https://doi.org/10.3390/diagnostics11091688 kostenfrei https://doaj.org/article/cd1a2c7b6520438abf000e49a5c3aea9 kostenfrei https://www.mdpi.com/2075-4418/11/9/1688 kostenfrei https://doaj.org/toc/2075-4418 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 9, p 1688
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allfieldsSound	10.3390/diagnostics11091688 doi (DE-627)DOAJ031014828 (DE-599)DOAJcd1a2c7b6520438abf000e49a5c3aea9 DE-627 ger DE-627 rakwb eng R5-920 Monika Lindemann verfasserin aut Comparison of Humoral and Cellular CMV Immunity in Patients Awaiting Kidney Transplantation 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chronic kidney disease may alter antiviral T cell immunity. In the current study, we assessed in 63 patients prior to kidney transplantation how humoral and cellular immunity against cytomegalovirus (CMV) correlated using an interferon (IFN)-γ ELISpot (T-Track<sup<®</sup< CMV, Mikrogen, Neuried, Germany). The cohort comprised 24 patients with negative and 39 with positive CMV IgG. Whereas none of the patients with negative CMV IgG showed detectable responses to the T-Track<sup<®</sup< CMV, 26 out of 39 patients with positive CMV IgG had positive ELISpot responses. The median response to CMV pp65 in the CMV seronegative group was 0 spot forming units (SFU) per 200,000 PBMC (range 0–1) and in the seropositive group 43 SFU (range 0–750). Thus, 13 out of 39 patients with positive CMV serostatus (33%) had undetectable T cell immunity and may be at an increased risk of CMV reactivation. CMV pp65-specific ELISpot responses were 29.3-fold higher in seropositive patients with vs. without dialysis and 5.6-fold higher in patients with vs. without immunosuppressive therapy, but patients with dialysis and immunosuppressive therapy showed, as expected, lower responses to phytohemagglutinin, the positive control. This finding may be caused by (subclinical) CMV-DNAemia and a “booster” of CMV-specific T cells. human cytomegalovirus ELISpot interferon-γ dialysis immunosuppressive therapy Medicine (General) Benjamin Wilde verfasserin aut Justa Friebus-Kardash verfasserin aut Anja Gäckler verfasserin aut Oliver Witzke verfasserin aut Ulf Dittmer verfasserin aut Peter A. Horn verfasserin aut Andreas Kribben verfasserin aut Nils Mülling verfasserin aut Ute Eisenberger verfasserin aut In Diagnostics MDPI AG, 2012 11(2021), 9, p 1688 (DE-627)718627814 (DE-600)2662336-5 20754418 nnns volume:11 year:2021 number:9, p 1688 https://doi.org/10.3390/diagnostics11091688 kostenfrei https://doaj.org/article/cd1a2c7b6520438abf000e49a5c3aea9 kostenfrei https://www.mdpi.com/2075-4418/11/9/1688 kostenfrei https://doaj.org/toc/2075-4418 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 9, p 1688
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authorswithroles_txt_mv	Monika Lindemann @@aut@@ Benjamin Wilde @@aut@@ Justa Friebus-Kardash @@aut@@ Anja Gäckler @@aut@@ Oliver Witzke @@aut@@ Ulf Dittmer @@aut@@ Peter A. Horn @@aut@@ Andreas Kribben @@aut@@ Nils Mülling @@aut@@ Ute Eisenberger @@aut@@
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callnumber-first	R - Medicine
author	Monika Lindemann
spellingShingle	Monika Lindemann misc R5-920 misc human cytomegalovirus misc ELISpot misc interferon-γ misc dialysis misc immunosuppressive therapy misc Medicine (General) Comparison of Humoral and Cellular CMV Immunity in Patients Awaiting Kidney Transplantation
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topic_title	R5-920 Comparison of Humoral and Cellular CMV Immunity in Patients Awaiting Kidney Transplantation human cytomegalovirus ELISpot interferon-γ dialysis immunosuppressive therapy
topic	misc R5-920 misc human cytomegalovirus misc ELISpot misc interferon-γ misc dialysis misc immunosuppressive therapy misc Medicine (General)
topic_unstemmed	misc R5-920 misc human cytomegalovirus misc ELISpot misc interferon-γ misc dialysis misc immunosuppressive therapy misc Medicine (General)
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title	Comparison of Humoral and Cellular CMV Immunity in Patients Awaiting Kidney Transplantation
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title_full	Comparison of Humoral and Cellular CMV Immunity in Patients Awaiting Kidney Transplantation
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author_browse	Monika Lindemann Benjamin Wilde Justa Friebus-Kardash Anja Gäckler Oliver Witzke Ulf Dittmer Peter A. Horn Andreas Kribben Nils Mülling Ute Eisenberger
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title_sort	comparison of humoral and cellular cmv immunity in patients awaiting kidney transplantation
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title_auth	Comparison of Humoral and Cellular CMV Immunity in Patients Awaiting Kidney Transplantation
abstract	Chronic kidney disease may alter antiviral T cell immunity. In the current study, we assessed in 63 patients prior to kidney transplantation how humoral and cellular immunity against cytomegalovirus (CMV) correlated using an interferon (IFN)-γ ELISpot (T-Track<sup<®</sup< CMV, Mikrogen, Neuried, Germany). The cohort comprised 24 patients with negative and 39 with positive CMV IgG. Whereas none of the patients with negative CMV IgG showed detectable responses to the T-Track<sup<®</sup< CMV, 26 out of 39 patients with positive CMV IgG had positive ELISpot responses. The median response to CMV pp65 in the CMV seronegative group was 0 spot forming units (SFU) per 200,000 PBMC (range 0–1) and in the seropositive group 43 SFU (range 0–750). Thus, 13 out of 39 patients with positive CMV serostatus (33%) had undetectable T cell immunity and may be at an increased risk of CMV reactivation. CMV pp65-specific ELISpot responses were 29.3-fold higher in seropositive patients with vs. without dialysis and 5.6-fold higher in patients with vs. without immunosuppressive therapy, but patients with dialysis and immunosuppressive therapy showed, as expected, lower responses to phytohemagglutinin, the positive control. This finding may be caused by (subclinical) CMV-DNAemia and a “booster” of CMV-specific T cells.
abstractGer	Chronic kidney disease may alter antiviral T cell immunity. In the current study, we assessed in 63 patients prior to kidney transplantation how humoral and cellular immunity against cytomegalovirus (CMV) correlated using an interferon (IFN)-γ ELISpot (T-Track<sup<®</sup< CMV, Mikrogen, Neuried, Germany). The cohort comprised 24 patients with negative and 39 with positive CMV IgG. Whereas none of the patients with negative CMV IgG showed detectable responses to the T-Track<sup<®</sup< CMV, 26 out of 39 patients with positive CMV IgG had positive ELISpot responses. The median response to CMV pp65 in the CMV seronegative group was 0 spot forming units (SFU) per 200,000 PBMC (range 0–1) and in the seropositive group 43 SFU (range 0–750). Thus, 13 out of 39 patients with positive CMV serostatus (33%) had undetectable T cell immunity and may be at an increased risk of CMV reactivation. CMV pp65-specific ELISpot responses were 29.3-fold higher in seropositive patients with vs. without dialysis and 5.6-fold higher in patients with vs. without immunosuppressive therapy, but patients with dialysis and immunosuppressive therapy showed, as expected, lower responses to phytohemagglutinin, the positive control. This finding may be caused by (subclinical) CMV-DNAemia and a “booster” of CMV-specific T cells.
abstract_unstemmed	Chronic kidney disease may alter antiviral T cell immunity. In the current study, we assessed in 63 patients prior to kidney transplantation how humoral and cellular immunity against cytomegalovirus (CMV) correlated using an interferon (IFN)-γ ELISpot (T-Track<sup<®</sup< CMV, Mikrogen, Neuried, Germany). The cohort comprised 24 patients with negative and 39 with positive CMV IgG. Whereas none of the patients with negative CMV IgG showed detectable responses to the T-Track<sup<®</sup< CMV, 26 out of 39 patients with positive CMV IgG had positive ELISpot responses. The median response to CMV pp65 in the CMV seronegative group was 0 spot forming units (SFU) per 200,000 PBMC (range 0–1) and in the seropositive group 43 SFU (range 0–750). Thus, 13 out of 39 patients with positive CMV serostatus (33%) had undetectable T cell immunity and may be at an increased risk of CMV reactivation. CMV pp65-specific ELISpot responses were 29.3-fold higher in seropositive patients with vs. without dialysis and 5.6-fold higher in patients with vs. without immunosuppressive therapy, but patients with dialysis and immunosuppressive therapy showed, as expected, lower responses to phytohemagglutinin, the positive control. This finding may be caused by (subclinical) CMV-DNAemia and a “booster” of CMV-specific T cells.
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title_short	Comparison of Humoral and Cellular CMV Immunity in Patients Awaiting Kidney Transplantation
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author2	Benjamin Wilde Justa Friebus-Kardash Anja Gäckler Oliver Witzke Ulf Dittmer Peter A. Horn Andreas Kribben Nils Mülling Ute Eisenberger
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up_date	2024-07-03T18:16:32.938Z
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In the current study, we assessed in 63 patients prior to kidney transplantation how humoral and cellular immunity against cytomegalovirus (CMV) correlated using an interferon (IFN)-γ ELISpot (T-Track<sup<®</sup< CMV, Mikrogen, Neuried, Germany). The cohort comprised 24 patients with negative and 39 with positive CMV IgG. Whereas none of the patients with negative CMV IgG showed detectable responses to the T-Track<sup<®</sup< CMV, 26 out of 39 patients with positive CMV IgG had positive ELISpot responses. The median response to CMV pp65 in the CMV seronegative group was 0 spot forming units (SFU) per 200,000 PBMC (range 0–1) and in the seropositive group 43 SFU (range 0–750). Thus, 13 out of 39 patients with positive CMV serostatus (33%) had undetectable T cell immunity and may be at an increased risk of CMV reactivation. CMV pp65-specific ELISpot responses were 29.3-fold higher in seropositive patients with vs. without dialysis and 5.6-fold higher in patients with vs. without immunosuppressive therapy, but patients with dialysis and immunosuppressive therapy showed, as expected, lower responses to phytohemagglutinin, the positive control. 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Comparison of Humoral and Cellular CMV Immunity in Patients Awaiting Kidney Transplantation

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