Inhibitory Effects of Cinnamaldehyde Derivatives on Biofilm Formation and Virulence Factors in <i<Vibrio</i< Species

<i<Vibrio parahaemolyticus</i< is considered one of the most relevant pathogenic marine bacteria with a range of virulence factors to establish food-related gastrointestinal infections in humans. Cinnamaldehyde (CNMA) and some of its derivatives have antimicrobial and antivirulence activities against several bacterial pathogens. This study examined the inhibitory effects of CNMA and its derivatives on biofilm formation and the virulence factors in <i<Vibrio</i< species, particularly <i<V. parahaemolyticus.</i< CNMA and ten of its derivatives were initially screened against <i<V. parahaemolyticus</i< biofilm formation, and their effects on the production of virulence factors and gene expression were studied. Among the CNMA derivatives tested, 4-nitrocinnamaldehyde, 4-chlorocinnamaldehyde, and 4-bromocinnamaldehyde displayed antibacterial and antivirulence activities, while the backbone CNMA had weak effects. The derivatives could prevent the adhesion of <i<V. parahaemolyticus</i< to surfaces by the dose-dependent inhibition of cell surface hydrophobicity, fimbriae production, and flagella-mediated swimming and swarming phenotypes. They also decreased the protease secretion required for virulence and indole production, which could act as an important signal molecule. The expression of QS and biofilm-related genes (<i<aphA</i<, <i<cpsA</i<, <i<luxS</i<, and o<i<paR</i<), virulence genes (<i<fliA</i<, <i<tdh</i<, and <i<vopS</i<), and membrane integrity genes (<i<fadL</i<, and <i<nusA</i<) were downregulated in <i<V. parahaemolyticus</i< by these three CNMA analogs. Interestingly, they eliminated <i<V. parahaemolyticus</i< and reduced the background flora from the squid surface. In addition, they exhibited similar antimicrobial and antibiofilm activities against <i<Vibrio harveyi</i<. This study identified CNMA derivatives as potential broad-spectrum antimicrobial agents to treat biofilm-mediated <i<Vibrio</i< infections and for surface disinfection in food processing facilities. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Olajide Sunday Faleye [verfasserIn] Ezhaveni Sathiyamoorthi [verfasserIn] Jin-Hyung Lee [verfasserIn] Jintae Lee [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	antibiofilm antivirulence cinnamaldehyde cinnamaldehyde derivatives

Übergeordnetes Werk:	In: Pharmaceutics - MDPI AG, 2010, 13(2021), 12, p 2176
Übergeordnetes Werk:	volume:13 ; year:2021 ; number:12, p 2176

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/pharmaceutics13122176

Katalog-ID:	DOAJ03122573X

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allfields	10.3390/pharmaceutics13122176 doi (DE-627)DOAJ03122573X (DE-599)DOAJ485159cfb32d44079c05f6d044122807 DE-627 ger DE-627 rakwb eng RS1-441 Olajide Sunday Faleye verfasserin aut Inhibitory Effects of Cinnamaldehyde Derivatives on Biofilm Formation and Virulence Factors in <i<Vibrio</i< Species 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Vibrio parahaemolyticus</i< is considered one of the most relevant pathogenic marine bacteria with a range of virulence factors to establish food-related gastrointestinal infections in humans. Cinnamaldehyde (CNMA) and some of its derivatives have antimicrobial and antivirulence activities against several bacterial pathogens. This study examined the inhibitory effects of CNMA and its derivatives on biofilm formation and the virulence factors in <i<Vibrio</i< species, particularly <i<V. parahaemolyticus.</i< CNMA and ten of its derivatives were initially screened against <i<V. parahaemolyticus</i< biofilm formation, and their effects on the production of virulence factors and gene expression were studied. Among the CNMA derivatives tested, 4-nitrocinnamaldehyde, 4-chlorocinnamaldehyde, and 4-bromocinnamaldehyde displayed antibacterial and antivirulence activities, while the backbone CNMA had weak effects. The derivatives could prevent the adhesion of <i<V. parahaemolyticus</i< to surfaces by the dose-dependent inhibition of cell surface hydrophobicity, fimbriae production, and flagella-mediated swimming and swarming phenotypes. They also decreased the protease secretion required for virulence and indole production, which could act as an important signal molecule. The expression of QS and biofilm-related genes (<i<aphA</i<, <i<cpsA</i<, <i<luxS</i<, and o<i<paR</i<), virulence genes (<i<fliA</i<, <i<tdh</i<, and <i<vopS</i<), and membrane integrity genes (<i<fadL</i<, and <i<nusA</i<) were downregulated in <i<V. parahaemolyticus</i< by these three CNMA analogs. Interestingly, they eliminated <i<V. parahaemolyticus</i< and reduced the background flora from the squid surface. In addition, they exhibited similar antimicrobial and antibiofilm activities against <i<Vibrio harveyi</i<. This study identified CNMA derivatives as potential broad-spectrum antimicrobial agents to treat biofilm-mediated <i<Vibrio</i< infections and for surface disinfection in food processing facilities. antibiofilm antivirulence cinnamaldehyde cinnamaldehyde derivatives <i<Vibrio parahaemolyticus</i< Pharmacy and materia medica Ezhaveni Sathiyamoorthi verfasserin aut Jin-Hyung Lee verfasserin aut Jintae Lee verfasserin aut In Pharmaceutics MDPI AG, 2010 13(2021), 12, p 2176 (DE-627)614096529 (DE-600)2527217-2 19994923 nnns volume:13 year:2021 number:12, p 2176 https://doi.org/10.3390/pharmaceutics13122176 kostenfrei https://doaj.org/article/485159cfb32d44079c05f6d044122807 kostenfrei https://www.mdpi.com/1999-4923/13/12/2176 kostenfrei https://doaj.org/toc/1999-4923 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2021 12, p 2176
spelling	10.3390/pharmaceutics13122176 doi (DE-627)DOAJ03122573X (DE-599)DOAJ485159cfb32d44079c05f6d044122807 DE-627 ger DE-627 rakwb eng RS1-441 Olajide Sunday Faleye verfasserin aut Inhibitory Effects of Cinnamaldehyde Derivatives on Biofilm Formation and Virulence Factors in <i<Vibrio</i< Species 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Vibrio parahaemolyticus</i< is considered one of the most relevant pathogenic marine bacteria with a range of virulence factors to establish food-related gastrointestinal infections in humans. Cinnamaldehyde (CNMA) and some of its derivatives have antimicrobial and antivirulence activities against several bacterial pathogens. This study examined the inhibitory effects of CNMA and its derivatives on biofilm formation and the virulence factors in <i<Vibrio</i< species, particularly <i<V. parahaemolyticus.</i< CNMA and ten of its derivatives were initially screened against <i<V. parahaemolyticus</i< biofilm formation, and their effects on the production of virulence factors and gene expression were studied. Among the CNMA derivatives tested, 4-nitrocinnamaldehyde, 4-chlorocinnamaldehyde, and 4-bromocinnamaldehyde displayed antibacterial and antivirulence activities, while the backbone CNMA had weak effects. The derivatives could prevent the adhesion of <i<V. parahaemolyticus</i< to surfaces by the dose-dependent inhibition of cell surface hydrophobicity, fimbriae production, and flagella-mediated swimming and swarming phenotypes. They also decreased the protease secretion required for virulence and indole production, which could act as an important signal molecule. The expression of QS and biofilm-related genes (<i<aphA</i<, <i<cpsA</i<, <i<luxS</i<, and o<i<paR</i<), virulence genes (<i<fliA</i<, <i<tdh</i<, and <i<vopS</i<), and membrane integrity genes (<i<fadL</i<, and <i<nusA</i<) were downregulated in <i<V. parahaemolyticus</i< by these three CNMA analogs. Interestingly, they eliminated <i<V. parahaemolyticus</i< and reduced the background flora from the squid surface. In addition, they exhibited similar antimicrobial and antibiofilm activities against <i<Vibrio harveyi</i<. This study identified CNMA derivatives as potential broad-spectrum antimicrobial agents to treat biofilm-mediated <i<Vibrio</i< infections and for surface disinfection in food processing facilities. antibiofilm antivirulence cinnamaldehyde cinnamaldehyde derivatives <i<Vibrio parahaemolyticus</i< Pharmacy and materia medica Ezhaveni Sathiyamoorthi verfasserin aut Jin-Hyung Lee verfasserin aut Jintae Lee verfasserin aut In Pharmaceutics MDPI AG, 2010 13(2021), 12, p 2176 (DE-627)614096529 (DE-600)2527217-2 19994923 nnns volume:13 year:2021 number:12, p 2176 https://doi.org/10.3390/pharmaceutics13122176 kostenfrei https://doaj.org/article/485159cfb32d44079c05f6d044122807 kostenfrei https://www.mdpi.com/1999-4923/13/12/2176 kostenfrei https://doaj.org/toc/1999-4923 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2021 12, p 2176
allfields_unstemmed	10.3390/pharmaceutics13122176 doi (DE-627)DOAJ03122573X (DE-599)DOAJ485159cfb32d44079c05f6d044122807 DE-627 ger DE-627 rakwb eng RS1-441 Olajide Sunday Faleye verfasserin aut Inhibitory Effects of Cinnamaldehyde Derivatives on Biofilm Formation and Virulence Factors in <i<Vibrio</i< Species 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Vibrio parahaemolyticus</i< is considered one of the most relevant pathogenic marine bacteria with a range of virulence factors to establish food-related gastrointestinal infections in humans. Cinnamaldehyde (CNMA) and some of its derivatives have antimicrobial and antivirulence activities against several bacterial pathogens. This study examined the inhibitory effects of CNMA and its derivatives on biofilm formation and the virulence factors in <i<Vibrio</i< species, particularly <i<V. parahaemolyticus.</i< CNMA and ten of its derivatives were initially screened against <i<V. parahaemolyticus</i< biofilm formation, and their effects on the production of virulence factors and gene expression were studied. Among the CNMA derivatives tested, 4-nitrocinnamaldehyde, 4-chlorocinnamaldehyde, and 4-bromocinnamaldehyde displayed antibacterial and antivirulence activities, while the backbone CNMA had weak effects. The derivatives could prevent the adhesion of <i<V. parahaemolyticus</i< to surfaces by the dose-dependent inhibition of cell surface hydrophobicity, fimbriae production, and flagella-mediated swimming and swarming phenotypes. They also decreased the protease secretion required for virulence and indole production, which could act as an important signal molecule. The expression of QS and biofilm-related genes (<i<aphA</i<, <i<cpsA</i<, <i<luxS</i<, and o<i<paR</i<), virulence genes (<i<fliA</i<, <i<tdh</i<, and <i<vopS</i<), and membrane integrity genes (<i<fadL</i<, and <i<nusA</i<) were downregulated in <i<V. parahaemolyticus</i< by these three CNMA analogs. Interestingly, they eliminated <i<V. parahaemolyticus</i< and reduced the background flora from the squid surface. In addition, they exhibited similar antimicrobial and antibiofilm activities against <i<Vibrio harveyi</i<. This study identified CNMA derivatives as potential broad-spectrum antimicrobial agents to treat biofilm-mediated <i<Vibrio</i< infections and for surface disinfection in food processing facilities. antibiofilm antivirulence cinnamaldehyde cinnamaldehyde derivatives <i<Vibrio parahaemolyticus</i< Pharmacy and materia medica Ezhaveni Sathiyamoorthi verfasserin aut Jin-Hyung Lee verfasserin aut Jintae Lee verfasserin aut In Pharmaceutics MDPI AG, 2010 13(2021), 12, p 2176 (DE-627)614096529 (DE-600)2527217-2 19994923 nnns volume:13 year:2021 number:12, p 2176 https://doi.org/10.3390/pharmaceutics13122176 kostenfrei https://doaj.org/article/485159cfb32d44079c05f6d044122807 kostenfrei https://www.mdpi.com/1999-4923/13/12/2176 kostenfrei https://doaj.org/toc/1999-4923 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2021 12, p 2176
allfieldsGer	10.3390/pharmaceutics13122176 doi (DE-627)DOAJ03122573X (DE-599)DOAJ485159cfb32d44079c05f6d044122807 DE-627 ger DE-627 rakwb eng RS1-441 Olajide Sunday Faleye verfasserin aut Inhibitory Effects of Cinnamaldehyde Derivatives on Biofilm Formation and Virulence Factors in <i<Vibrio</i< Species 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Vibrio parahaemolyticus</i< is considered one of the most relevant pathogenic marine bacteria with a range of virulence factors to establish food-related gastrointestinal infections in humans. Cinnamaldehyde (CNMA) and some of its derivatives have antimicrobial and antivirulence activities against several bacterial pathogens. This study examined the inhibitory effects of CNMA and its derivatives on biofilm formation and the virulence factors in <i<Vibrio</i< species, particularly <i<V. parahaemolyticus.</i< CNMA and ten of its derivatives were initially screened against <i<V. parahaemolyticus</i< biofilm formation, and their effects on the production of virulence factors and gene expression were studied. Among the CNMA derivatives tested, 4-nitrocinnamaldehyde, 4-chlorocinnamaldehyde, and 4-bromocinnamaldehyde displayed antibacterial and antivirulence activities, while the backbone CNMA had weak effects. The derivatives could prevent the adhesion of <i<V. parahaemolyticus</i< to surfaces by the dose-dependent inhibition of cell surface hydrophobicity, fimbriae production, and flagella-mediated swimming and swarming phenotypes. They also decreased the protease secretion required for virulence and indole production, which could act as an important signal molecule. The expression of QS and biofilm-related genes (<i<aphA</i<, <i<cpsA</i<, <i<luxS</i<, and o<i<paR</i<), virulence genes (<i<fliA</i<, <i<tdh</i<, and <i<vopS</i<), and membrane integrity genes (<i<fadL</i<, and <i<nusA</i<) were downregulated in <i<V. parahaemolyticus</i< by these three CNMA analogs. Interestingly, they eliminated <i<V. parahaemolyticus</i< and reduced the background flora from the squid surface. In addition, they exhibited similar antimicrobial and antibiofilm activities against <i<Vibrio harveyi</i<. This study identified CNMA derivatives as potential broad-spectrum antimicrobial agents to treat biofilm-mediated <i<Vibrio</i< infections and for surface disinfection in food processing facilities. antibiofilm antivirulence cinnamaldehyde cinnamaldehyde derivatives <i<Vibrio parahaemolyticus</i< Pharmacy and materia medica Ezhaveni Sathiyamoorthi verfasserin aut Jin-Hyung Lee verfasserin aut Jintae Lee verfasserin aut In Pharmaceutics MDPI AG, 2010 13(2021), 12, p 2176 (DE-627)614096529 (DE-600)2527217-2 19994923 nnns volume:13 year:2021 number:12, p 2176 https://doi.org/10.3390/pharmaceutics13122176 kostenfrei https://doaj.org/article/485159cfb32d44079c05f6d044122807 kostenfrei https://www.mdpi.com/1999-4923/13/12/2176 kostenfrei https://doaj.org/toc/1999-4923 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2021 12, p 2176
allfieldsSound	10.3390/pharmaceutics13122176 doi (DE-627)DOAJ03122573X (DE-599)DOAJ485159cfb32d44079c05f6d044122807 DE-627 ger DE-627 rakwb eng RS1-441 Olajide Sunday Faleye verfasserin aut Inhibitory Effects of Cinnamaldehyde Derivatives on Biofilm Formation and Virulence Factors in <i<Vibrio</i< Species 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Vibrio parahaemolyticus</i< is considered one of the most relevant pathogenic marine bacteria with a range of virulence factors to establish food-related gastrointestinal infections in humans. Cinnamaldehyde (CNMA) and some of its derivatives have antimicrobial and antivirulence activities against several bacterial pathogens. This study examined the inhibitory effects of CNMA and its derivatives on biofilm formation and the virulence factors in <i<Vibrio</i< species, particularly <i<V. parahaemolyticus.</i< CNMA and ten of its derivatives were initially screened against <i<V. parahaemolyticus</i< biofilm formation, and their effects on the production of virulence factors and gene expression were studied. Among the CNMA derivatives tested, 4-nitrocinnamaldehyde, 4-chlorocinnamaldehyde, and 4-bromocinnamaldehyde displayed antibacterial and antivirulence activities, while the backbone CNMA had weak effects. The derivatives could prevent the adhesion of <i<V. parahaemolyticus</i< to surfaces by the dose-dependent inhibition of cell surface hydrophobicity, fimbriae production, and flagella-mediated swimming and swarming phenotypes. They also decreased the protease secretion required for virulence and indole production, which could act as an important signal molecule. The expression of QS and biofilm-related genes (<i<aphA</i<, <i<cpsA</i<, <i<luxS</i<, and o<i<paR</i<), virulence genes (<i<fliA</i<, <i<tdh</i<, and <i<vopS</i<), and membrane integrity genes (<i<fadL</i<, and <i<nusA</i<) were downregulated in <i<V. parahaemolyticus</i< by these three CNMA analogs. Interestingly, they eliminated <i<V. parahaemolyticus</i< and reduced the background flora from the squid surface. In addition, they exhibited similar antimicrobial and antibiofilm activities against <i<Vibrio harveyi</i<. This study identified CNMA derivatives as potential broad-spectrum antimicrobial agents to treat biofilm-mediated <i<Vibrio</i< infections and for surface disinfection in food processing facilities. antibiofilm antivirulence cinnamaldehyde cinnamaldehyde derivatives <i<Vibrio parahaemolyticus</i< Pharmacy and materia medica Ezhaveni Sathiyamoorthi verfasserin aut Jin-Hyung Lee verfasserin aut Jintae Lee verfasserin aut In Pharmaceutics MDPI AG, 2010 13(2021), 12, p 2176 (DE-627)614096529 (DE-600)2527217-2 19994923 nnns volume:13 year:2021 number:12, p 2176 https://doi.org/10.3390/pharmaceutics13122176 kostenfrei https://doaj.org/article/485159cfb32d44079c05f6d044122807 kostenfrei https://www.mdpi.com/1999-4923/13/12/2176 kostenfrei https://doaj.org/toc/1999-4923 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2021 12, p 2176
language	English
source	In Pharmaceutics 13(2021), 12, p 2176 volume:13 year:2021 number:12, p 2176
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topic_facet	antibiofilm antivirulence cinnamaldehyde cinnamaldehyde derivatives <i<Vibrio parahaemolyticus</i< Pharmacy and materia medica
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container_title	Pharmaceutics
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topic_title	RS1-441 Inhibitory Effects of Cinnamaldehyde Derivatives on Biofilm Formation and Virulence Factors in <i<Vibrio</i< Species antibiofilm antivirulence cinnamaldehyde cinnamaldehyde derivatives <i<Vibrio parahaemolyticus</i<
topic	misc RS1-441 misc antibiofilm misc antivirulence misc cinnamaldehyde misc cinnamaldehyde derivatives misc <i<Vibrio parahaemolyticus</i< misc Pharmacy and materia medica
topic_unstemmed	misc RS1-441 misc antibiofilm misc antivirulence misc cinnamaldehyde misc cinnamaldehyde derivatives misc <i<Vibrio parahaemolyticus</i< misc Pharmacy and materia medica
topic_browse	misc RS1-441 misc antibiofilm misc antivirulence misc cinnamaldehyde misc cinnamaldehyde derivatives misc <i<Vibrio parahaemolyticus</i< misc Pharmacy and materia medica
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title	Inhibitory Effects of Cinnamaldehyde Derivatives on Biofilm Formation and Virulence Factors in <i<Vibrio</i< Species
ctrlnum	(DE-627)DOAJ03122573X (DE-599)DOAJ485159cfb32d44079c05f6d044122807
title_full	Inhibitory Effects of Cinnamaldehyde Derivatives on Biofilm Formation and Virulence Factors in <i<Vibrio</i< Species
author_sort	Olajide Sunday Faleye
journal	Pharmaceutics
journalStr	Pharmaceutics
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lang_code	eng
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publishDateSort	2021
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author_browse	Olajide Sunday Faleye Ezhaveni Sathiyamoorthi Jin-Hyung Lee Jintae Lee
container_volume	13
class	RS1-441
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author-letter	Olajide Sunday Faleye
doi_str_mv	10.3390/pharmaceutics13122176
author2-role	verfasserin
title_sort	inhibitory effects of cinnamaldehyde derivatives on biofilm formation and virulence factors in <i<vibrio</i< species
callnumber	RS1-441
title_auth	Inhibitory Effects of Cinnamaldehyde Derivatives on Biofilm Formation and Virulence Factors in <i<Vibrio</i< Species
abstract	<i<Vibrio parahaemolyticus</i< is considered one of the most relevant pathogenic marine bacteria with a range of virulence factors to establish food-related gastrointestinal infections in humans. Cinnamaldehyde (CNMA) and some of its derivatives have antimicrobial and antivirulence activities against several bacterial pathogens. This study examined the inhibitory effects of CNMA and its derivatives on biofilm formation and the virulence factors in <i<Vibrio</i< species, particularly <i<V. parahaemolyticus.</i< CNMA and ten of its derivatives were initially screened against <i<V. parahaemolyticus</i< biofilm formation, and their effects on the production of virulence factors and gene expression were studied. Among the CNMA derivatives tested, 4-nitrocinnamaldehyde, 4-chlorocinnamaldehyde, and 4-bromocinnamaldehyde displayed antibacterial and antivirulence activities, while the backbone CNMA had weak effects. The derivatives could prevent the adhesion of <i<V. parahaemolyticus</i< to surfaces by the dose-dependent inhibition of cell surface hydrophobicity, fimbriae production, and flagella-mediated swimming and swarming phenotypes. They also decreased the protease secretion required for virulence and indole production, which could act as an important signal molecule. The expression of QS and biofilm-related genes (<i<aphA</i<, <i<cpsA</i<, <i<luxS</i<, and o<i<paR</i<), virulence genes (<i<fliA</i<, <i<tdh</i<, and <i<vopS</i<), and membrane integrity genes (<i<fadL</i<, and <i<nusA</i<) were downregulated in <i<V. parahaemolyticus</i< by these three CNMA analogs. Interestingly, they eliminated <i<V. parahaemolyticus</i< and reduced the background flora from the squid surface. In addition, they exhibited similar antimicrobial and antibiofilm activities against <i<Vibrio harveyi</i<. This study identified CNMA derivatives as potential broad-spectrum antimicrobial agents to treat biofilm-mediated <i<Vibrio</i< infections and for surface disinfection in food processing facilities.
abstractGer	<i<Vibrio parahaemolyticus</i< is considered one of the most relevant pathogenic marine bacteria with a range of virulence factors to establish food-related gastrointestinal infections in humans. Cinnamaldehyde (CNMA) and some of its derivatives have antimicrobial and antivirulence activities against several bacterial pathogens. This study examined the inhibitory effects of CNMA and its derivatives on biofilm formation and the virulence factors in <i<Vibrio</i< species, particularly <i<V. parahaemolyticus.</i< CNMA and ten of its derivatives were initially screened against <i<V. parahaemolyticus</i< biofilm formation, and their effects on the production of virulence factors and gene expression were studied. Among the CNMA derivatives tested, 4-nitrocinnamaldehyde, 4-chlorocinnamaldehyde, and 4-bromocinnamaldehyde displayed antibacterial and antivirulence activities, while the backbone CNMA had weak effects. The derivatives could prevent the adhesion of <i<V. parahaemolyticus</i< to surfaces by the dose-dependent inhibition of cell surface hydrophobicity, fimbriae production, and flagella-mediated swimming and swarming phenotypes. They also decreased the protease secretion required for virulence and indole production, which could act as an important signal molecule. The expression of QS and biofilm-related genes (<i<aphA</i<, <i<cpsA</i<, <i<luxS</i<, and o<i<paR</i<), virulence genes (<i<fliA</i<, <i<tdh</i<, and <i<vopS</i<), and membrane integrity genes (<i<fadL</i<, and <i<nusA</i<) were downregulated in <i<V. parahaemolyticus</i< by these three CNMA analogs. Interestingly, they eliminated <i<V. parahaemolyticus</i< and reduced the background flora from the squid surface. In addition, they exhibited similar antimicrobial and antibiofilm activities against <i<Vibrio harveyi</i<. This study identified CNMA derivatives as potential broad-spectrum antimicrobial agents to treat biofilm-mediated <i<Vibrio</i< infections and for surface disinfection in food processing facilities.
abstract_unstemmed	<i<Vibrio parahaemolyticus</i< is considered one of the most relevant pathogenic marine bacteria with a range of virulence factors to establish food-related gastrointestinal infections in humans. Cinnamaldehyde (CNMA) and some of its derivatives have antimicrobial and antivirulence activities against several bacterial pathogens. This study examined the inhibitory effects of CNMA and its derivatives on biofilm formation and the virulence factors in <i<Vibrio</i< species, particularly <i<V. parahaemolyticus.</i< CNMA and ten of its derivatives were initially screened against <i<V. parahaemolyticus</i< biofilm formation, and their effects on the production of virulence factors and gene expression were studied. Among the CNMA derivatives tested, 4-nitrocinnamaldehyde, 4-chlorocinnamaldehyde, and 4-bromocinnamaldehyde displayed antibacterial and antivirulence activities, while the backbone CNMA had weak effects. The derivatives could prevent the adhesion of <i<V. parahaemolyticus</i< to surfaces by the dose-dependent inhibition of cell surface hydrophobicity, fimbriae production, and flagella-mediated swimming and swarming phenotypes. They also decreased the protease secretion required for virulence and indole production, which could act as an important signal molecule. The expression of QS and biofilm-related genes (<i<aphA</i<, <i<cpsA</i<, <i<luxS</i<, and o<i<paR</i<), virulence genes (<i<fliA</i<, <i<tdh</i<, and <i<vopS</i<), and membrane integrity genes (<i<fadL</i<, and <i<nusA</i<) were downregulated in <i<V. parahaemolyticus</i< by these three CNMA analogs. Interestingly, they eliminated <i<V. parahaemolyticus</i< and reduced the background flora from the squid surface. In addition, they exhibited similar antimicrobial and antibiofilm activities against <i<Vibrio harveyi</i<. This study identified CNMA derivatives as potential broad-spectrum antimicrobial agents to treat biofilm-mediated <i<Vibrio</i< infections and for surface disinfection in food processing facilities.
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title_short	Inhibitory Effects of Cinnamaldehyde Derivatives on Biofilm Formation and Virulence Factors in <i<Vibrio</i< Species
url	https://doi.org/10.3390/pharmaceutics13122176 https://doaj.org/article/485159cfb32d44079c05f6d044122807 https://www.mdpi.com/1999-4923/13/12/2176 https://doaj.org/toc/1999-4923
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author2	Ezhaveni Sathiyamoorthi Jin-Hyung Lee Jintae Lee
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up_date	2024-07-03T19:26:40.650Z
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