Genetic Polymorphism of the Wild and In vitro Regenerated Plants of the Medicinal Grass Cymbopogon schoenanthus subsp. proximus

Cymbopogon schoenanthus subsp. proximus grows wild in subtropical Africa, Sudan and Egypt. The species is heavily collected for its use in folk medicine and drug production. A wild population from south Egypt was used to determine preliminary genetic polymorphism within the species, using nineteen I...
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Autor*in:	Asmaa M. ABDELSALAM [verfasserIn] Kamal CHOWDHURY [verfasserIn] Ahmed A. El-BAKRY [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	cpSSR; ISSR; organogenesis; RAPD; somatic embryogenesis

Übergeordnetes Werk:	In: Notulae Scientia Biologicae - Society of Land Measurements and Cadastre from Transylvania (SMTCT), 2009, 11(2019), 2
Übergeordnetes Werk:	volume:11 ; year:2019 ; number:2

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.15835/nsb11210470

Katalog-ID:	DOAJ031567479

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520			\|a Cymbopogon schoenanthus subsp. proximus grows wild in subtropical Africa, Sudan and Egypt. The species is heavily collected for its use in folk medicine and drug production. A wild population from south Egypt was used to determine preliminary genetic polymorphism within the species, using nineteen ISSR, fourteen RAPD and seven cpSSR primers. Three regeneration systems, somatic embryogenesis (SE), direct organogenesis (D), and indirect organogenesis (ID), were established from seed explants of the same population and polymorphism within regenerated plants was determined. ISSR generated a total of 222 amplified fragments for all genotypes, while RAPDs and cpSSR yielded 139 and 34 fragments, respectively. Wild plants showed an average low polymorphism for all marker types of 45.8%. Regenerated plants polymorphism was also low (SE=44.6, D=44, ID=46.2%). ISSR and cpSSR markers were more sensitive in elucidating polymorphism (51.5 and 46.87%) than RAPD (37.85%). ISSR was the most significant marker in producing unique bands, for the wild genotypes (6), SE (7), D (5) and ID (6). cpSSR followed producing 4 for wild genotypes, 6 for SE, 3 for D and 6 for ID organogenesis. Unweighted pair group with arithmetic average (UPGMA) clustering analysis and Jaccard’s similarity data suggested that wild plants and those regenerated through somatic embryogenesis and direct organogenesis are more similar. The study elucidated low polymorphism within both the wild population and regenerated genotypes, with plants regenerated through somatic embryogenesis and direct organogenesis being more similar to wild plant genotypes, suggesting their future use in studies with genetic transformation and ex-situ conservation of the species.
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spelling	10.15835/nsb11210470 doi (DE-627)DOAJ031567479 (DE-599)DOAJ14b2e02571fd44e1a0c97880d5ecc4af DE-627 ger DE-627 rakwb eng S1-972 Q1-390 Asmaa M. ABDELSALAM verfasserin aut Genetic Polymorphism of the Wild and In vitro Regenerated Plants of the Medicinal Grass Cymbopogon schoenanthus subsp. proximus 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cymbopogon schoenanthus subsp. proximus grows wild in subtropical Africa, Sudan and Egypt. The species is heavily collected for its use in folk medicine and drug production. A wild population from south Egypt was used to determine preliminary genetic polymorphism within the species, using nineteen ISSR, fourteen RAPD and seven cpSSR primers. Three regeneration systems, somatic embryogenesis (SE), direct organogenesis (D), and indirect organogenesis (ID), were established from seed explants of the same population and polymorphism within regenerated plants was determined. ISSR generated a total of 222 amplified fragments for all genotypes, while RAPDs and cpSSR yielded 139 and 34 fragments, respectively. Wild plants showed an average low polymorphism for all marker types of 45.8%. Regenerated plants polymorphism was also low (SE=44.6, D=44, ID=46.2%). ISSR and cpSSR markers were more sensitive in elucidating polymorphism (51.5 and 46.87%) than RAPD (37.85%). ISSR was the most significant marker in producing unique bands, for the wild genotypes (6), SE (7), D (5) and ID (6). cpSSR followed producing 4 for wild genotypes, 6 for SE, 3 for D and 6 for ID organogenesis. Unweighted pair group with arithmetic average (UPGMA) clustering analysis and Jaccard’s similarity data suggested that wild plants and those regenerated through somatic embryogenesis and direct organogenesis are more similar. The study elucidated low polymorphism within both the wild population and regenerated genotypes, with plants regenerated through somatic embryogenesis and direct organogenesis being more similar to wild plant genotypes, suggesting their future use in studies with genetic transformation and ex-situ conservation of the species. cpSSR; ISSR; organogenesis; RAPD; somatic embryogenesis Agriculture (General) Science (General) Kamal CHOWDHURY verfasserin aut Ahmed A. El-BAKRY verfasserin aut In Notulae Scientia Biologicae Society of Land Measurements and Cadastre from Transylvania (SMTCT), 2009 11(2019), 2 (DE-627)617090882 (DE-600)2533002-0 20673264 nnns volume:11 year:2019 number:2 https://doi.org/10.15835/nsb11210470 kostenfrei https://doaj.org/article/14b2e02571fd44e1a0c97880d5ecc4af kostenfrei https://www.notulaebiologicae.ro/index.php/nsb/article/view/10470 kostenfrei https://doaj.org/toc/2067-3205 Journal toc kostenfrei https://doaj.org/toc/2067-3264 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2019 2
allfields_unstemmed	10.15835/nsb11210470 doi (DE-627)DOAJ031567479 (DE-599)DOAJ14b2e02571fd44e1a0c97880d5ecc4af DE-627 ger DE-627 rakwb eng S1-972 Q1-390 Asmaa M. ABDELSALAM verfasserin aut Genetic Polymorphism of the Wild and In vitro Regenerated Plants of the Medicinal Grass Cymbopogon schoenanthus subsp. proximus 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cymbopogon schoenanthus subsp. proximus grows wild in subtropical Africa, Sudan and Egypt. The species is heavily collected for its use in folk medicine and drug production. A wild population from south Egypt was used to determine preliminary genetic polymorphism within the species, using nineteen ISSR, fourteen RAPD and seven cpSSR primers. Three regeneration systems, somatic embryogenesis (SE), direct organogenesis (D), and indirect organogenesis (ID), were established from seed explants of the same population and polymorphism within regenerated plants was determined. ISSR generated a total of 222 amplified fragments for all genotypes, while RAPDs and cpSSR yielded 139 and 34 fragments, respectively. Wild plants showed an average low polymorphism for all marker types of 45.8%. Regenerated plants polymorphism was also low (SE=44.6, D=44, ID=46.2%). ISSR and cpSSR markers were more sensitive in elucidating polymorphism (51.5 and 46.87%) than RAPD (37.85%). ISSR was the most significant marker in producing unique bands, for the wild genotypes (6), SE (7), D (5) and ID (6). cpSSR followed producing 4 for wild genotypes, 6 for SE, 3 for D and 6 for ID organogenesis. Unweighted pair group with arithmetic average (UPGMA) clustering analysis and Jaccard’s similarity data suggested that wild plants and those regenerated through somatic embryogenesis and direct organogenesis are more similar. The study elucidated low polymorphism within both the wild population and regenerated genotypes, with plants regenerated through somatic embryogenesis and direct organogenesis being more similar to wild plant genotypes, suggesting their future use in studies with genetic transformation and ex-situ conservation of the species. cpSSR; ISSR; organogenesis; RAPD; somatic embryogenesis Agriculture (General) Science (General) Kamal CHOWDHURY verfasserin aut Ahmed A. El-BAKRY verfasserin aut In Notulae Scientia Biologicae Society of Land Measurements and Cadastre from Transylvania (SMTCT), 2009 11(2019), 2 (DE-627)617090882 (DE-600)2533002-0 20673264 nnns volume:11 year:2019 number:2 https://doi.org/10.15835/nsb11210470 kostenfrei https://doaj.org/article/14b2e02571fd44e1a0c97880d5ecc4af kostenfrei https://www.notulaebiologicae.ro/index.php/nsb/article/view/10470 kostenfrei https://doaj.org/toc/2067-3205 Journal toc kostenfrei https://doaj.org/toc/2067-3264 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2019 2
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allfieldsSound	10.15835/nsb11210470 doi (DE-627)DOAJ031567479 (DE-599)DOAJ14b2e02571fd44e1a0c97880d5ecc4af DE-627 ger DE-627 rakwb eng S1-972 Q1-390 Asmaa M. ABDELSALAM verfasserin aut Genetic Polymorphism of the Wild and In vitro Regenerated Plants of the Medicinal Grass Cymbopogon schoenanthus subsp. proximus 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cymbopogon schoenanthus subsp. proximus grows wild in subtropical Africa, Sudan and Egypt. The species is heavily collected for its use in folk medicine and drug production. A wild population from south Egypt was used to determine preliminary genetic polymorphism within the species, using nineteen ISSR, fourteen RAPD and seven cpSSR primers. Three regeneration systems, somatic embryogenesis (SE), direct organogenesis (D), and indirect organogenesis (ID), were established from seed explants of the same population and polymorphism within regenerated plants was determined. ISSR generated a total of 222 amplified fragments for all genotypes, while RAPDs and cpSSR yielded 139 and 34 fragments, respectively. Wild plants showed an average low polymorphism for all marker types of 45.8%. Regenerated plants polymorphism was also low (SE=44.6, D=44, ID=46.2%). ISSR and cpSSR markers were more sensitive in elucidating polymorphism (51.5 and 46.87%) than RAPD (37.85%). ISSR was the most significant marker in producing unique bands, for the wild genotypes (6), SE (7), D (5) and ID (6). cpSSR followed producing 4 for wild genotypes, 6 for SE, 3 for D and 6 for ID organogenesis. Unweighted pair group with arithmetic average (UPGMA) clustering analysis and Jaccard’s similarity data suggested that wild plants and those regenerated through somatic embryogenesis and direct organogenesis are more similar. The study elucidated low polymorphism within both the wild population and regenerated genotypes, with plants regenerated through somatic embryogenesis and direct organogenesis being more similar to wild plant genotypes, suggesting their future use in studies with genetic transformation and ex-situ conservation of the species. cpSSR; ISSR; organogenesis; RAPD; somatic embryogenesis Agriculture (General) Science (General) Kamal CHOWDHURY verfasserin aut Ahmed A. El-BAKRY verfasserin aut In Notulae Scientia Biologicae Society of Land Measurements and Cadastre from Transylvania (SMTCT), 2009 11(2019), 2 (DE-627)617090882 (DE-600)2533002-0 20673264 nnns volume:11 year:2019 number:2 https://doi.org/10.15835/nsb11210470 kostenfrei https://doaj.org/article/14b2e02571fd44e1a0c97880d5ecc4af kostenfrei https://www.notulaebiologicae.ro/index.php/nsb/article/view/10470 kostenfrei https://doaj.org/toc/2067-3205 Journal toc kostenfrei https://doaj.org/toc/2067-3264 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2019 2
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spellingShingle	Asmaa M. ABDELSALAM misc S1-972 misc Q1-390 misc cpSSR; ISSR; organogenesis; RAPD; somatic embryogenesis misc Agriculture (General) misc Science (General) Genetic Polymorphism of the Wild and In vitro Regenerated Plants of the Medicinal Grass Cymbopogon schoenanthus subsp. proximus
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title	Genetic Polymorphism of the Wild and In vitro Regenerated Plants of the Medicinal Grass Cymbopogon schoenanthus subsp. proximus
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title_full	Genetic Polymorphism of the Wild and In vitro Regenerated Plants of the Medicinal Grass Cymbopogon schoenanthus subsp. proximus
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title_sort	genetic polymorphism of the wild and in vitro regenerated plants of the medicinal grass cymbopogon schoenanthus subsp. proximus
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title_auth	Genetic Polymorphism of the Wild and In vitro Regenerated Plants of the Medicinal Grass Cymbopogon schoenanthus subsp. proximus
abstract	Cymbopogon schoenanthus subsp. proximus grows wild in subtropical Africa, Sudan and Egypt. The species is heavily collected for its use in folk medicine and drug production. A wild population from south Egypt was used to determine preliminary genetic polymorphism within the species, using nineteen ISSR, fourteen RAPD and seven cpSSR primers. Three regeneration systems, somatic embryogenesis (SE), direct organogenesis (D), and indirect organogenesis (ID), were established from seed explants of the same population and polymorphism within regenerated plants was determined. ISSR generated a total of 222 amplified fragments for all genotypes, while RAPDs and cpSSR yielded 139 and 34 fragments, respectively. Wild plants showed an average low polymorphism for all marker types of 45.8%. Regenerated plants polymorphism was also low (SE=44.6, D=44, ID=46.2%). ISSR and cpSSR markers were more sensitive in elucidating polymorphism (51.5 and 46.87%) than RAPD (37.85%). ISSR was the most significant marker in producing unique bands, for the wild genotypes (6), SE (7), D (5) and ID (6). cpSSR followed producing 4 for wild genotypes, 6 for SE, 3 for D and 6 for ID organogenesis. Unweighted pair group with arithmetic average (UPGMA) clustering analysis and Jaccard’s similarity data suggested that wild plants and those regenerated through somatic embryogenesis and direct organogenesis are more similar. The study elucidated low polymorphism within both the wild population and regenerated genotypes, with plants regenerated through somatic embryogenesis and direct organogenesis being more similar to wild plant genotypes, suggesting their future use in studies with genetic transformation and ex-situ conservation of the species.
abstractGer	Cymbopogon schoenanthus subsp. proximus grows wild in subtropical Africa, Sudan and Egypt. The species is heavily collected for its use in folk medicine and drug production. A wild population from south Egypt was used to determine preliminary genetic polymorphism within the species, using nineteen ISSR, fourteen RAPD and seven cpSSR primers. Three regeneration systems, somatic embryogenesis (SE), direct organogenesis (D), and indirect organogenesis (ID), were established from seed explants of the same population and polymorphism within regenerated plants was determined. ISSR generated a total of 222 amplified fragments for all genotypes, while RAPDs and cpSSR yielded 139 and 34 fragments, respectively. Wild plants showed an average low polymorphism for all marker types of 45.8%. Regenerated plants polymorphism was also low (SE=44.6, D=44, ID=46.2%). ISSR and cpSSR markers were more sensitive in elucidating polymorphism (51.5 and 46.87%) than RAPD (37.85%). ISSR was the most significant marker in producing unique bands, for the wild genotypes (6), SE (7), D (5) and ID (6). cpSSR followed producing 4 for wild genotypes, 6 for SE, 3 for D and 6 for ID organogenesis. Unweighted pair group with arithmetic average (UPGMA) clustering analysis and Jaccard’s similarity data suggested that wild plants and those regenerated through somatic embryogenesis and direct organogenesis are more similar. The study elucidated low polymorphism within both the wild population and regenerated genotypes, with plants regenerated through somatic embryogenesis and direct organogenesis being more similar to wild plant genotypes, suggesting their future use in studies with genetic transformation and ex-situ conservation of the species.
abstract_unstemmed	Cymbopogon schoenanthus subsp. proximus grows wild in subtropical Africa, Sudan and Egypt. The species is heavily collected for its use in folk medicine and drug production. A wild population from south Egypt was used to determine preliminary genetic polymorphism within the species, using nineteen ISSR, fourteen RAPD and seven cpSSR primers. Three regeneration systems, somatic embryogenesis (SE), direct organogenesis (D), and indirect organogenesis (ID), were established from seed explants of the same population and polymorphism within regenerated plants was determined. ISSR generated a total of 222 amplified fragments for all genotypes, while RAPDs and cpSSR yielded 139 and 34 fragments, respectively. Wild plants showed an average low polymorphism for all marker types of 45.8%. Regenerated plants polymorphism was also low (SE=44.6, D=44, ID=46.2%). ISSR and cpSSR markers were more sensitive in elucidating polymorphism (51.5 and 46.87%) than RAPD (37.85%). ISSR was the most significant marker in producing unique bands, for the wild genotypes (6), SE (7), D (5) and ID (6). cpSSR followed producing 4 for wild genotypes, 6 for SE, 3 for D and 6 for ID organogenesis. Unweighted pair group with arithmetic average (UPGMA) clustering analysis and Jaccard’s similarity data suggested that wild plants and those regenerated through somatic embryogenesis and direct organogenesis are more similar. The study elucidated low polymorphism within both the wild population and regenerated genotypes, with plants regenerated through somatic embryogenesis and direct organogenesis being more similar to wild plant genotypes, suggesting their future use in studies with genetic transformation and ex-situ conservation of the species.
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title_short	Genetic Polymorphism of the Wild and In vitro Regenerated Plants of the Medicinal Grass Cymbopogon schoenanthus subsp. proximus
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ISSR was the most significant marker in producing unique bands, for the wild genotypes (6), SE (7), D (5) and ID (6). cpSSR followed producing 4 for wild genotypes, 6 for SE, 3 for D and 6 for ID organogenesis. Unweighted pair group with arithmetic average (UPGMA) clustering analysis and Jaccard’s similarity data suggested that wild plants and those regenerated through somatic embryogenesis and direct organogenesis are more similar. 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Genetic Polymorphism of the Wild and In vitro Regenerated Plants of the Medicinal Grass Cymbopogon schoenanthus subsp. proximus

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