Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026

Quorum sensing signals are referred to as acylated homoserine lactones (AHL) that are mainly found in Gram-negative bacteria. It implies the ability of certain bacteria of producing different AHL molecules. The bacteria Pseudomonas aureofaciens and Xenorhabdus nematophila were cultured in Luria-Bert...
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Autor*in:	Ahmad Humayan KABIR [verfasserIn] Anindya GHOSH ROY [verfasserIn] Mohammad FIROZ ALAM [verfasserIn] Rafiul ISLAM [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2010

Übergeordnetes Werk:	In: Notulae Scientia Biologicae - Society of Land Measurements and Cadastre from Transylvania (SMTCT), 2009, 2(2010), 4, Seite 72-75
Übergeordnetes Werk:	volume:2 ; year:2010 ; number:4 ; pages:72-75

Links:	Link aufrufen Link aufrufen Journal toc Journal toc

Katalog-ID:	DOAJ031599680

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allfields	(DE-627)DOAJ031599680 (DE-599)DOAJ8478679b670a44268be155a8315dd93d DE-627 ger DE-627 rakwb eng S1-972 Q1-390 Ahmad Humayan KABIR verfasserin aut Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Quorum sensing signals are referred to as acylated homoserine lactones (AHL) that are mainly found in Gram-negative bacteria. It implies the ability of certain bacteria of producing different AHL molecules. The bacteria Pseudomonas aureofaciens and Xenorhabdus nematophila were cultured in Luria-Bertani (LB10) media and CV026 was used as a reporter strain to detect the presence of AHLs produced by the cultured bacteria. In this study, the reporter strain has revealed the quorum sensing ability of P. aureofaciens and X. nematophila by producing the purple pigment violacein in the supply of external AHLs molecules. Thin layer chromatography (TLC) bioassay having four controls was conducted to detect specific AHL molecule supplied by P. aureofaciens and X. nematophila. The specific AHL molecule was observed to be migrated according to their polarity on the TLC plate. Agriculture (General) Science (General) Anindya GHOSH ROY verfasserin aut Mohammad FIROZ ALAM verfasserin aut Rafiul ISLAM verfasserin aut In Notulae Scientia Biologicae Society of Land Measurements and Cadastre from Transylvania (SMTCT), 2009 2(2010), 4, Seite 72-75 (DE-627)617090882 (DE-600)2533002-0 20673264 nnns volume:2 year:2010 number:4 pages:72-75 https://doaj.org/article/8478679b670a44268be155a8315dd93d kostenfrei http://notulaebiologicae.ro/index.php/nsb/article/view/4863 kostenfrei https://doaj.org/toc/2067-3205 Journal toc kostenfrei https://doaj.org/toc/2067-3264 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2010 4 72-75
spelling	(DE-627)DOAJ031599680 (DE-599)DOAJ8478679b670a44268be155a8315dd93d DE-627 ger DE-627 rakwb eng S1-972 Q1-390 Ahmad Humayan KABIR verfasserin aut Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Quorum sensing signals are referred to as acylated homoserine lactones (AHL) that are mainly found in Gram-negative bacteria. It implies the ability of certain bacteria of producing different AHL molecules. The bacteria Pseudomonas aureofaciens and Xenorhabdus nematophila were cultured in Luria-Bertani (LB10) media and CV026 was used as a reporter strain to detect the presence of AHLs produced by the cultured bacteria. In this study, the reporter strain has revealed the quorum sensing ability of P. aureofaciens and X. nematophila by producing the purple pigment violacein in the supply of external AHLs molecules. Thin layer chromatography (TLC) bioassay having four controls was conducted to detect specific AHL molecule supplied by P. aureofaciens and X. nematophila. The specific AHL molecule was observed to be migrated according to their polarity on the TLC plate. Agriculture (General) Science (General) Anindya GHOSH ROY verfasserin aut Mohammad FIROZ ALAM verfasserin aut Rafiul ISLAM verfasserin aut In Notulae Scientia Biologicae Society of Land Measurements and Cadastre from Transylvania (SMTCT), 2009 2(2010), 4, Seite 72-75 (DE-627)617090882 (DE-600)2533002-0 20673264 nnns volume:2 year:2010 number:4 pages:72-75 https://doaj.org/article/8478679b670a44268be155a8315dd93d kostenfrei http://notulaebiologicae.ro/index.php/nsb/article/view/4863 kostenfrei https://doaj.org/toc/2067-3205 Journal toc kostenfrei https://doaj.org/toc/2067-3264 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2010 4 72-75
allfields_unstemmed	(DE-627)DOAJ031599680 (DE-599)DOAJ8478679b670a44268be155a8315dd93d DE-627 ger DE-627 rakwb eng S1-972 Q1-390 Ahmad Humayan KABIR verfasserin aut Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Quorum sensing signals are referred to as acylated homoserine lactones (AHL) that are mainly found in Gram-negative bacteria. It implies the ability of certain bacteria of producing different AHL molecules. The bacteria Pseudomonas aureofaciens and Xenorhabdus nematophila were cultured in Luria-Bertani (LB10) media and CV026 was used as a reporter strain to detect the presence of AHLs produced by the cultured bacteria. In this study, the reporter strain has revealed the quorum sensing ability of P. aureofaciens and X. nematophila by producing the purple pigment violacein in the supply of external AHLs molecules. Thin layer chromatography (TLC) bioassay having four controls was conducted to detect specific AHL molecule supplied by P. aureofaciens and X. nematophila. The specific AHL molecule was observed to be migrated according to their polarity on the TLC plate. Agriculture (General) Science (General) Anindya GHOSH ROY verfasserin aut Mohammad FIROZ ALAM verfasserin aut Rafiul ISLAM verfasserin aut In Notulae Scientia Biologicae Society of Land Measurements and Cadastre from Transylvania (SMTCT), 2009 2(2010), 4, Seite 72-75 (DE-627)617090882 (DE-600)2533002-0 20673264 nnns volume:2 year:2010 number:4 pages:72-75 https://doaj.org/article/8478679b670a44268be155a8315dd93d kostenfrei http://notulaebiologicae.ro/index.php/nsb/article/view/4863 kostenfrei https://doaj.org/toc/2067-3205 Journal toc kostenfrei https://doaj.org/toc/2067-3264 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2010 4 72-75
allfieldsGer	(DE-627)DOAJ031599680 (DE-599)DOAJ8478679b670a44268be155a8315dd93d DE-627 ger DE-627 rakwb eng S1-972 Q1-390 Ahmad Humayan KABIR verfasserin aut Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Quorum sensing signals are referred to as acylated homoserine lactones (AHL) that are mainly found in Gram-negative bacteria. It implies the ability of certain bacteria of producing different AHL molecules. The bacteria Pseudomonas aureofaciens and Xenorhabdus nematophila were cultured in Luria-Bertani (LB10) media and CV026 was used as a reporter strain to detect the presence of AHLs produced by the cultured bacteria. In this study, the reporter strain has revealed the quorum sensing ability of P. aureofaciens and X. nematophila by producing the purple pigment violacein in the supply of external AHLs molecules. Thin layer chromatography (TLC) bioassay having four controls was conducted to detect specific AHL molecule supplied by P. aureofaciens and X. nematophila. The specific AHL molecule was observed to be migrated according to their polarity on the TLC plate. Agriculture (General) Science (General) Anindya GHOSH ROY verfasserin aut Mohammad FIROZ ALAM verfasserin aut Rafiul ISLAM verfasserin aut In Notulae Scientia Biologicae Society of Land Measurements and Cadastre from Transylvania (SMTCT), 2009 2(2010), 4, Seite 72-75 (DE-627)617090882 (DE-600)2533002-0 20673264 nnns volume:2 year:2010 number:4 pages:72-75 https://doaj.org/article/8478679b670a44268be155a8315dd93d kostenfrei http://notulaebiologicae.ro/index.php/nsb/article/view/4863 kostenfrei https://doaj.org/toc/2067-3205 Journal toc kostenfrei https://doaj.org/toc/2067-3264 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2010 4 72-75
allfieldsSound	(DE-627)DOAJ031599680 (DE-599)DOAJ8478679b670a44268be155a8315dd93d DE-627 ger DE-627 rakwb eng S1-972 Q1-390 Ahmad Humayan KABIR verfasserin aut Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Quorum sensing signals are referred to as acylated homoserine lactones (AHL) that are mainly found in Gram-negative bacteria. It implies the ability of certain bacteria of producing different AHL molecules. The bacteria Pseudomonas aureofaciens and Xenorhabdus nematophila were cultured in Luria-Bertani (LB10) media and CV026 was used as a reporter strain to detect the presence of AHLs produced by the cultured bacteria. In this study, the reporter strain has revealed the quorum sensing ability of P. aureofaciens and X. nematophila by producing the purple pigment violacein in the supply of external AHLs molecules. Thin layer chromatography (TLC) bioassay having four controls was conducted to detect specific AHL molecule supplied by P. aureofaciens and X. nematophila. The specific AHL molecule was observed to be migrated according to their polarity on the TLC plate. Agriculture (General) Science (General) Anindya GHOSH ROY verfasserin aut Mohammad FIROZ ALAM verfasserin aut Rafiul ISLAM verfasserin aut In Notulae Scientia Biologicae Society of Land Measurements and Cadastre from Transylvania (SMTCT), 2009 2(2010), 4, Seite 72-75 (DE-627)617090882 (DE-600)2533002-0 20673264 nnns volume:2 year:2010 number:4 pages:72-75 https://doaj.org/article/8478679b670a44268be155a8315dd93d kostenfrei http://notulaebiologicae.ro/index.php/nsb/article/view/4863 kostenfrei https://doaj.org/toc/2067-3205 Journal toc kostenfrei https://doaj.org/toc/2067-3264 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2010 4 72-75
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callnumber-first	S - Agriculture
author	Ahmad Humayan KABIR
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title	Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026
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title_full	Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026
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title_sort	detection of quorum sensing signals in gram-negative bacteria by using reporter strain cv026
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title_auth	Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026
abstract	Quorum sensing signals are referred to as acylated homoserine lactones (AHL) that are mainly found in Gram-negative bacteria. It implies the ability of certain bacteria of producing different AHL molecules. The bacteria Pseudomonas aureofaciens and Xenorhabdus nematophila were cultured in Luria-Bertani (LB10) media and CV026 was used as a reporter strain to detect the presence of AHLs produced by the cultured bacteria. In this study, the reporter strain has revealed the quorum sensing ability of P. aureofaciens and X. nematophila by producing the purple pigment violacein in the supply of external AHLs molecules. Thin layer chromatography (TLC) bioassay having four controls was conducted to detect specific AHL molecule supplied by P. aureofaciens and X. nematophila. The specific AHL molecule was observed to be migrated according to their polarity on the TLC plate.
abstractGer	Quorum sensing signals are referred to as acylated homoserine lactones (AHL) that are mainly found in Gram-negative bacteria. It implies the ability of certain bacteria of producing different AHL molecules. The bacteria Pseudomonas aureofaciens and Xenorhabdus nematophila were cultured in Luria-Bertani (LB10) media and CV026 was used as a reporter strain to detect the presence of AHLs produced by the cultured bacteria. In this study, the reporter strain has revealed the quorum sensing ability of P. aureofaciens and X. nematophila by producing the purple pigment violacein in the supply of external AHLs molecules. Thin layer chromatography (TLC) bioassay having four controls was conducted to detect specific AHL molecule supplied by P. aureofaciens and X. nematophila. The specific AHL molecule was observed to be migrated according to their polarity on the TLC plate.
abstract_unstemmed	Quorum sensing signals are referred to as acylated homoserine lactones (AHL) that are mainly found in Gram-negative bacteria. It implies the ability of certain bacteria of producing different AHL molecules. The bacteria Pseudomonas aureofaciens and Xenorhabdus nematophila were cultured in Luria-Bertani (LB10) media and CV026 was used as a reporter strain to detect the presence of AHLs produced by the cultured bacteria. In this study, the reporter strain has revealed the quorum sensing ability of P. aureofaciens and X. nematophila by producing the purple pigment violacein in the supply of external AHLs molecules. Thin layer chromatography (TLC) bioassay having four controls was conducted to detect specific AHL molecule supplied by P. aureofaciens and X. nematophila. The specific AHL molecule was observed to be migrated according to their polarity on the TLC plate.
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title_short	Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026
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