In vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (Dc.) Hochst (Fabaceae)

Abstract Background Piliostigma reticulatum is a plant traditionally used to treat malaria, smallpox, neuralgia, dysentery, diarrhea, and rheumatism in Northern Nigeria. There is no scientific evidence to support the antimalarial activity of this plant. This work aims to investigate the in vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (MPR) in mice, infected with NK65 chloroquine-sensitive Plasmodium berghei. The oral lethal doses and preliminary phytochemical screening of the extract were performed. The therapeutic, suppressive, and prophylactic models were used for the antiplasmodial activity at the doses of 250, 500, and 1000 mg/kg of the MPR extract. Chloroquine and artesunate were used as the positive control drugs, while distilled water was used for the negative control group. The antiplasmodial activity was determined by comparing the mean parasite clearance in the treated groups, to the negative control group. Also the effect of the extract on the blood packed-cell volume of mice (PCV) was determined. Results The LD50 of MPR was found to be < 5000 mg/kg. Glycosides, saponins, tannins, flavonoids, triterpenes and alkaloids were the phytochemicals identified in the extract. The extract of MPR produced a significant reduction in the mean parasitemia level compared to the negative control in the curative test: MPR 250 (68.31%, P < 0.001), MPR 500 (76.53%, P < 0.001), and MPR1000 (83.65%, P < 0.001). The extract prolonged the survival of infected mice (18.8 days), compared to the negative control (5.2 days). The extract produced significant chemosuppression compared to the negative control; MPR 250 (73.79%, P < 0.001), MPR 500 (81.33%, P < 0.001), and MPR 1000 (78.37%, P < 0.001). The extract produced significant chemoprophylaxis compared to the negative control; MPR 250 (68.5%, P < 0.001), MPR 500 (58.7%, P < 0.001), and MPR 1000 (84.77%, P < 0.001). The extract was found to have no significant effect on the blood PCV of the treated groups compared to the negative control. Conclusions The study showed that the MPR extract has significant antiplasmodial activity in mice at the doses tested, and could justify the traditional use of the plant in the treatment of malaria in Northern Nigeria. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Safiya Shehu Abdulkadir [verfasserIn] Abubakar Ibrahim Jatau [verfasserIn] Umar Sharif Abdussalam [verfasserIn] Lawal Alhassan Bichi [verfasserIn] Bilyaminu Abubakar [verfasserIn] Sani Malami [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	In vivo antiplasmodial Plasmodium berghei Piliostigma reticulatum Mice

Übergeordnetes Werk:	In: Bulletin of the National Research Centre - SpringerOpen, 2018, 46(2022), 1, Seite 8
Übergeordnetes Werk:	volume:46 ; year:2022 ; number:1 ; pages:8

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/s42269-022-00910-0

Katalog-ID:	DOAJ03180120X

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245	1	0	\|a In vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (Dc.) Hochst (Fabaceae)
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520			\|a Abstract Background Piliostigma reticulatum is a plant traditionally used to treat malaria, smallpox, neuralgia, dysentery, diarrhea, and rheumatism in Northern Nigeria. There is no scientific evidence to support the antimalarial activity of this plant. This work aims to investigate the in vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (MPR) in mice, infected with NK65 chloroquine-sensitive Plasmodium berghei. The oral lethal doses and preliminary phytochemical screening of the extract were performed. The therapeutic, suppressive, and prophylactic models were used for the antiplasmodial activity at the doses of 250, 500, and 1000 mg/kg of the MPR extract. Chloroquine and artesunate were used as the positive control drugs, while distilled water was used for the negative control group. The antiplasmodial activity was determined by comparing the mean parasite clearance in the treated groups, to the negative control group. Also the effect of the extract on the blood packed-cell volume of mice (PCV) was determined. Results The LD50 of MPR was found to be < 5000 mg/kg. Glycosides, saponins, tannins, flavonoids, triterpenes and alkaloids were the phytochemicals identified in the extract. The extract of MPR produced a significant reduction in the mean parasitemia level compared to the negative control in the curative test: MPR 250 (68.31%, P < 0.001), MPR 500 (76.53%, P < 0.001), and MPR1000 (83.65%, P < 0.001). The extract prolonged the survival of infected mice (18.8 days), compared to the negative control (5.2 days). The extract produced significant chemosuppression compared to the negative control; MPR 250 (73.79%, P < 0.001), MPR 500 (81.33%, P < 0.001), and MPR 1000 (78.37%, P < 0.001). The extract produced significant chemoprophylaxis compared to the negative control; MPR 250 (68.5%, P < 0.001), MPR 500 (58.7%, P < 0.001), and MPR 1000 (84.77%, P < 0.001). The extract was found to have no significant effect on the blood PCV of the treated groups compared to the negative control. Conclusions The study showed that the MPR extract has significant antiplasmodial activity in mice at the doses tested, and could justify the traditional use of the plant in the treatment of malaria in Northern Nigeria.
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author_variant	s s a ssa a i j aij u s a usa l a b lab b a ba s m sm
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allfields	10.1186/s42269-022-00910-0 doi (DE-627)DOAJ03180120X (DE-599)DOAJc808ab845c21418e98128402757e9dff DE-627 ger DE-627 rakwb eng Safiya Shehu Abdulkadir verfasserin aut In vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (Dc.) Hochst (Fabaceae) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Piliostigma reticulatum is a plant traditionally used to treat malaria, smallpox, neuralgia, dysentery, diarrhea, and rheumatism in Northern Nigeria. There is no scientific evidence to support the antimalarial activity of this plant. This work aims to investigate the in vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (MPR) in mice, infected with NK65 chloroquine-sensitive Plasmodium berghei. The oral lethal doses and preliminary phytochemical screening of the extract were performed. The therapeutic, suppressive, and prophylactic models were used for the antiplasmodial activity at the doses of 250, 500, and 1000 mg/kg of the MPR extract. Chloroquine and artesunate were used as the positive control drugs, while distilled water was used for the negative control group. The antiplasmodial activity was determined by comparing the mean parasite clearance in the treated groups, to the negative control group. Also the effect of the extract on the blood packed-cell volume of mice (PCV) was determined. Results The LD50 of MPR was found to be < 5000 mg/kg. Glycosides, saponins, tannins, flavonoids, triterpenes and alkaloids were the phytochemicals identified in the extract. The extract of MPR produced a significant reduction in the mean parasitemia level compared to the negative control in the curative test: MPR 250 (68.31%, P < 0.001), MPR 500 (76.53%, P < 0.001), and MPR1000 (83.65%, P < 0.001). The extract prolonged the survival of infected mice (18.8 days), compared to the negative control (5.2 days). The extract produced significant chemosuppression compared to the negative control; MPR 250 (73.79%, P < 0.001), MPR 500 (81.33%, P < 0.001), and MPR 1000 (78.37%, P < 0.001). The extract produced significant chemoprophylaxis compared to the negative control; MPR 250 (68.5%, P < 0.001), MPR 500 (58.7%, P < 0.001), and MPR 1000 (84.77%, P < 0.001). The extract was found to have no significant effect on the blood PCV of the treated groups compared to the negative control. Conclusions The study showed that the MPR extract has significant antiplasmodial activity in mice at the doses tested, and could justify the traditional use of the plant in the treatment of malaria in Northern Nigeria. In vivo antiplasmodial Plasmodium berghei Piliostigma reticulatum Mice Science Q Abubakar Ibrahim Jatau verfasserin aut Umar Sharif Abdussalam verfasserin aut Lawal Alhassan Bichi verfasserin aut Bilyaminu Abubakar verfasserin aut Sani Malami verfasserin aut In Bulletin of the National Research Centre SpringerOpen, 2018 46(2022), 1, Seite 8 (DE-627)1035877007 25228307 nnns volume:46 year:2022 number:1 pages:8 https://doi.org/10.1186/s42269-022-00910-0 kostenfrei https://doaj.org/article/c808ab845c21418e98128402757e9dff kostenfrei https://doi.org/10.1186/s42269-022-00910-0 kostenfrei https://doaj.org/toc/2522-8307 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 46 2022 1 8
spelling	10.1186/s42269-022-00910-0 doi (DE-627)DOAJ03180120X (DE-599)DOAJc808ab845c21418e98128402757e9dff DE-627 ger DE-627 rakwb eng Safiya Shehu Abdulkadir verfasserin aut In vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (Dc.) Hochst (Fabaceae) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Piliostigma reticulatum is a plant traditionally used to treat malaria, smallpox, neuralgia, dysentery, diarrhea, and rheumatism in Northern Nigeria. There is no scientific evidence to support the antimalarial activity of this plant. This work aims to investigate the in vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (MPR) in mice, infected with NK65 chloroquine-sensitive Plasmodium berghei. The oral lethal doses and preliminary phytochemical screening of the extract were performed. The therapeutic, suppressive, and prophylactic models were used for the antiplasmodial activity at the doses of 250, 500, and 1000 mg/kg of the MPR extract. Chloroquine and artesunate were used as the positive control drugs, while distilled water was used for the negative control group. The antiplasmodial activity was determined by comparing the mean parasite clearance in the treated groups, to the negative control group. Also the effect of the extract on the blood packed-cell volume of mice (PCV) was determined. Results The LD50 of MPR was found to be < 5000 mg/kg. Glycosides, saponins, tannins, flavonoids, triterpenes and alkaloids were the phytochemicals identified in the extract. The extract of MPR produced a significant reduction in the mean parasitemia level compared to the negative control in the curative test: MPR 250 (68.31%, P < 0.001), MPR 500 (76.53%, P < 0.001), and MPR1000 (83.65%, P < 0.001). The extract prolonged the survival of infected mice (18.8 days), compared to the negative control (5.2 days). The extract produced significant chemosuppression compared to the negative control; MPR 250 (73.79%, P < 0.001), MPR 500 (81.33%, P < 0.001), and MPR 1000 (78.37%, P < 0.001). The extract produced significant chemoprophylaxis compared to the negative control; MPR 250 (68.5%, P < 0.001), MPR 500 (58.7%, P < 0.001), and MPR 1000 (84.77%, P < 0.001). The extract was found to have no significant effect on the blood PCV of the treated groups compared to the negative control. Conclusions The study showed that the MPR extract has significant antiplasmodial activity in mice at the doses tested, and could justify the traditional use of the plant in the treatment of malaria in Northern Nigeria. In vivo antiplasmodial Plasmodium berghei Piliostigma reticulatum Mice Science Q Abubakar Ibrahim Jatau verfasserin aut Umar Sharif Abdussalam verfasserin aut Lawal Alhassan Bichi verfasserin aut Bilyaminu Abubakar verfasserin aut Sani Malami verfasserin aut In Bulletin of the National Research Centre SpringerOpen, 2018 46(2022), 1, Seite 8 (DE-627)1035877007 25228307 nnns volume:46 year:2022 number:1 pages:8 https://doi.org/10.1186/s42269-022-00910-0 kostenfrei https://doaj.org/article/c808ab845c21418e98128402757e9dff kostenfrei https://doi.org/10.1186/s42269-022-00910-0 kostenfrei https://doaj.org/toc/2522-8307 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 46 2022 1 8
allfields_unstemmed	10.1186/s42269-022-00910-0 doi (DE-627)DOAJ03180120X (DE-599)DOAJc808ab845c21418e98128402757e9dff DE-627 ger DE-627 rakwb eng Safiya Shehu Abdulkadir verfasserin aut In vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (Dc.) Hochst (Fabaceae) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Piliostigma reticulatum is a plant traditionally used to treat malaria, smallpox, neuralgia, dysentery, diarrhea, and rheumatism in Northern Nigeria. There is no scientific evidence to support the antimalarial activity of this plant. This work aims to investigate the in vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (MPR) in mice, infected with NK65 chloroquine-sensitive Plasmodium berghei. The oral lethal doses and preliminary phytochemical screening of the extract were performed. The therapeutic, suppressive, and prophylactic models were used for the antiplasmodial activity at the doses of 250, 500, and 1000 mg/kg of the MPR extract. Chloroquine and artesunate were used as the positive control drugs, while distilled water was used for the negative control group. The antiplasmodial activity was determined by comparing the mean parasite clearance in the treated groups, to the negative control group. Also the effect of the extract on the blood packed-cell volume of mice (PCV) was determined. Results The LD50 of MPR was found to be < 5000 mg/kg. Glycosides, saponins, tannins, flavonoids, triterpenes and alkaloids were the phytochemicals identified in the extract. The extract of MPR produced a significant reduction in the mean parasitemia level compared to the negative control in the curative test: MPR 250 (68.31%, P < 0.001), MPR 500 (76.53%, P < 0.001), and MPR1000 (83.65%, P < 0.001). The extract prolonged the survival of infected mice (18.8 days), compared to the negative control (5.2 days). The extract produced significant chemosuppression compared to the negative control; MPR 250 (73.79%, P < 0.001), MPR 500 (81.33%, P < 0.001), and MPR 1000 (78.37%, P < 0.001). The extract produced significant chemoprophylaxis compared to the negative control; MPR 250 (68.5%, P < 0.001), MPR 500 (58.7%, P < 0.001), and MPR 1000 (84.77%, P < 0.001). The extract was found to have no significant effect on the blood PCV of the treated groups compared to the negative control. Conclusions The study showed that the MPR extract has significant antiplasmodial activity in mice at the doses tested, and could justify the traditional use of the plant in the treatment of malaria in Northern Nigeria. In vivo antiplasmodial Plasmodium berghei Piliostigma reticulatum Mice Science Q Abubakar Ibrahim Jatau verfasserin aut Umar Sharif Abdussalam verfasserin aut Lawal Alhassan Bichi verfasserin aut Bilyaminu Abubakar verfasserin aut Sani Malami verfasserin aut In Bulletin of the National Research Centre SpringerOpen, 2018 46(2022), 1, Seite 8 (DE-627)1035877007 25228307 nnns volume:46 year:2022 number:1 pages:8 https://doi.org/10.1186/s42269-022-00910-0 kostenfrei https://doaj.org/article/c808ab845c21418e98128402757e9dff kostenfrei https://doi.org/10.1186/s42269-022-00910-0 kostenfrei https://doaj.org/toc/2522-8307 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 46 2022 1 8
allfieldsGer	10.1186/s42269-022-00910-0 doi (DE-627)DOAJ03180120X (DE-599)DOAJc808ab845c21418e98128402757e9dff DE-627 ger DE-627 rakwb eng Safiya Shehu Abdulkadir verfasserin aut In vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (Dc.) Hochst (Fabaceae) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Piliostigma reticulatum is a plant traditionally used to treat malaria, smallpox, neuralgia, dysentery, diarrhea, and rheumatism in Northern Nigeria. There is no scientific evidence to support the antimalarial activity of this plant. This work aims to investigate the in vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (MPR) in mice, infected with NK65 chloroquine-sensitive Plasmodium berghei. The oral lethal doses and preliminary phytochemical screening of the extract were performed. The therapeutic, suppressive, and prophylactic models were used for the antiplasmodial activity at the doses of 250, 500, and 1000 mg/kg of the MPR extract. Chloroquine and artesunate were used as the positive control drugs, while distilled water was used for the negative control group. The antiplasmodial activity was determined by comparing the mean parasite clearance in the treated groups, to the negative control group. Also the effect of the extract on the blood packed-cell volume of mice (PCV) was determined. Results The LD50 of MPR was found to be < 5000 mg/kg. Glycosides, saponins, tannins, flavonoids, triterpenes and alkaloids were the phytochemicals identified in the extract. The extract of MPR produced a significant reduction in the mean parasitemia level compared to the negative control in the curative test: MPR 250 (68.31%, P < 0.001), MPR 500 (76.53%, P < 0.001), and MPR1000 (83.65%, P < 0.001). The extract prolonged the survival of infected mice (18.8 days), compared to the negative control (5.2 days). The extract produced significant chemosuppression compared to the negative control; MPR 250 (73.79%, P < 0.001), MPR 500 (81.33%, P < 0.001), and MPR 1000 (78.37%, P < 0.001). The extract produced significant chemoprophylaxis compared to the negative control; MPR 250 (68.5%, P < 0.001), MPR 500 (58.7%, P < 0.001), and MPR 1000 (84.77%, P < 0.001). The extract was found to have no significant effect on the blood PCV of the treated groups compared to the negative control. Conclusions The study showed that the MPR extract has significant antiplasmodial activity in mice at the doses tested, and could justify the traditional use of the plant in the treatment of malaria in Northern Nigeria. In vivo antiplasmodial Plasmodium berghei Piliostigma reticulatum Mice Science Q Abubakar Ibrahim Jatau verfasserin aut Umar Sharif Abdussalam verfasserin aut Lawal Alhassan Bichi verfasserin aut Bilyaminu Abubakar verfasserin aut Sani Malami verfasserin aut In Bulletin of the National Research Centre SpringerOpen, 2018 46(2022), 1, Seite 8 (DE-627)1035877007 25228307 nnns volume:46 year:2022 number:1 pages:8 https://doi.org/10.1186/s42269-022-00910-0 kostenfrei https://doaj.org/article/c808ab845c21418e98128402757e9dff kostenfrei https://doi.org/10.1186/s42269-022-00910-0 kostenfrei https://doaj.org/toc/2522-8307 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 46 2022 1 8
allfieldsSound	10.1186/s42269-022-00910-0 doi (DE-627)DOAJ03180120X (DE-599)DOAJc808ab845c21418e98128402757e9dff DE-627 ger DE-627 rakwb eng Safiya Shehu Abdulkadir verfasserin aut In vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (Dc.) Hochst (Fabaceae) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Piliostigma reticulatum is a plant traditionally used to treat malaria, smallpox, neuralgia, dysentery, diarrhea, and rheumatism in Northern Nigeria. There is no scientific evidence to support the antimalarial activity of this plant. This work aims to investigate the in vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (MPR) in mice, infected with NK65 chloroquine-sensitive Plasmodium berghei. The oral lethal doses and preliminary phytochemical screening of the extract were performed. The therapeutic, suppressive, and prophylactic models were used for the antiplasmodial activity at the doses of 250, 500, and 1000 mg/kg of the MPR extract. Chloroquine and artesunate were used as the positive control drugs, while distilled water was used for the negative control group. The antiplasmodial activity was determined by comparing the mean parasite clearance in the treated groups, to the negative control group. Also the effect of the extract on the blood packed-cell volume of mice (PCV) was determined. Results The LD50 of MPR was found to be < 5000 mg/kg. Glycosides, saponins, tannins, flavonoids, triterpenes and alkaloids were the phytochemicals identified in the extract. The extract of MPR produced a significant reduction in the mean parasitemia level compared to the negative control in the curative test: MPR 250 (68.31%, P < 0.001), MPR 500 (76.53%, P < 0.001), and MPR1000 (83.65%, P < 0.001). The extract prolonged the survival of infected mice (18.8 days), compared to the negative control (5.2 days). The extract produced significant chemosuppression compared to the negative control; MPR 250 (73.79%, P < 0.001), MPR 500 (81.33%, P < 0.001), and MPR 1000 (78.37%, P < 0.001). The extract produced significant chemoprophylaxis compared to the negative control; MPR 250 (68.5%, P < 0.001), MPR 500 (58.7%, P < 0.001), and MPR 1000 (84.77%, P < 0.001). The extract was found to have no significant effect on the blood PCV of the treated groups compared to the negative control. Conclusions The study showed that the MPR extract has significant antiplasmodial activity in mice at the doses tested, and could justify the traditional use of the plant in the treatment of malaria in Northern Nigeria. In vivo antiplasmodial Plasmodium berghei Piliostigma reticulatum Mice Science Q Abubakar Ibrahim Jatau verfasserin aut Umar Sharif Abdussalam verfasserin aut Lawal Alhassan Bichi verfasserin aut Bilyaminu Abubakar verfasserin aut Sani Malami verfasserin aut In Bulletin of the National Research Centre SpringerOpen, 2018 46(2022), 1, Seite 8 (DE-627)1035877007 25228307 nnns volume:46 year:2022 number:1 pages:8 https://doi.org/10.1186/s42269-022-00910-0 kostenfrei https://doaj.org/article/c808ab845c21418e98128402757e9dff kostenfrei https://doi.org/10.1186/s42269-022-00910-0 kostenfrei https://doaj.org/toc/2522-8307 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 46 2022 1 8
language	English
source	In Bulletin of the National Research Centre 46(2022), 1, Seite 8 volume:46 year:2022 number:1 pages:8
sourceStr	In Bulletin of the National Research Centre 46(2022), 1, Seite 8 volume:46 year:2022 number:1 pages:8
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	In vivo antiplasmodial Plasmodium berghei Piliostigma reticulatum Mice Science Q
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container_title	Bulletin of the National Research Centre
authorswithroles_txt_mv	Safiya Shehu Abdulkadir @@aut@@ Abubakar Ibrahim Jatau @@aut@@ Umar Sharif Abdussalam @@aut@@ Lawal Alhassan Bichi @@aut@@ Bilyaminu Abubakar @@aut@@ Sani Malami @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	1035877007
id	DOAJ03180120X
language_de	englisch
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Hochst (Fabaceae)</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2022</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Background Piliostigma reticulatum is a plant traditionally used to treat malaria, smallpox, neuralgia, dysentery, diarrhea, and rheumatism in Northern Nigeria. There is no scientific evidence to support the antimalarial activity of this plant. This work aims to investigate the in vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (MPR) in mice, infected with NK65 chloroquine-sensitive Plasmodium berghei. The oral lethal doses and preliminary phytochemical screening of the extract were performed. The therapeutic, suppressive, and prophylactic models were used for the antiplasmodial activity at the doses of 250, 500, and 1000 mg/kg of the MPR extract. Chloroquine and artesunate were used as the positive control drugs, while distilled water was used for the negative control group. The antiplasmodial activity was determined by comparing the mean parasite clearance in the treated groups, to the negative control group. Also the effect of the extract on the blood packed-cell volume of mice (PCV) was determined. Results The LD50 of MPR was found to be < 5000 mg/kg. Glycosides, saponins, tannins, flavonoids, triterpenes and alkaloids were the phytochemicals identified in the extract. The extract of MPR produced a significant reduction in the mean parasitemia level compared to the negative control in the curative test: MPR 250 (68.31%, P < 0.001), MPR 500 (76.53%, P < 0.001), and MPR1000 (83.65%, P < 0.001). The extract prolonged the survival of infected mice (18.8 days), compared to the negative control (5.2 days). The extract produced significant chemosuppression compared to the negative control; MPR 250 (73.79%, P < 0.001), MPR 500 (81.33%, P < 0.001), and MPR 1000 (78.37%, P < 0.001). The extract produced significant chemoprophylaxis compared to the negative control; MPR 250 (68.5%, P < 0.001), MPR 500 (58.7%, P < 0.001), and MPR 1000 (84.77%, P < 0.001). The extract was found to have no significant effect on the blood PCV of the treated groups compared to the negative control. Conclusions The study showed that the MPR extract has significant antiplasmodial activity in mice at the doses tested, and could justify the traditional use of the plant in the treatment of malaria in Northern Nigeria.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">In vivo antiplasmodial</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Plasmodium berghei</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Piliostigma reticulatum</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Mice</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Science</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Q</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Abubakar Ibrahim Jatau</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" 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author	Safiya Shehu Abdulkadir
spellingShingle	Safiya Shehu Abdulkadir misc In vivo antiplasmodial misc Plasmodium berghei misc Piliostigma reticulatum misc Mice misc Science misc Q In vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (Dc.) Hochst (Fabaceae)
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topic_title	In vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (Dc.) Hochst (Fabaceae) In vivo antiplasmodial Plasmodium berghei Piliostigma reticulatum Mice
topic	misc In vivo antiplasmodial misc Plasmodium berghei misc Piliostigma reticulatum misc Mice misc Science misc Q
topic_unstemmed	misc In vivo antiplasmodial misc Plasmodium berghei misc Piliostigma reticulatum misc Mice misc Science misc Q
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title	In vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (Dc.) Hochst (Fabaceae)
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title_full	In vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (Dc.) Hochst (Fabaceae)
author_sort	Safiya Shehu Abdulkadir
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author_browse	Safiya Shehu Abdulkadir Abubakar Ibrahim Jatau Umar Sharif Abdussalam Lawal Alhassan Bichi Bilyaminu Abubakar Sani Malami
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doi_str_mv	10.1186/s42269-022-00910-0
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title_sort	in vivo antiplasmodial activity of the methanol leaf extract of piliostigma reticulatum (dc.) hochst (fabaceae)
title_auth	In vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (Dc.) Hochst (Fabaceae)
abstract	Abstract Background Piliostigma reticulatum is a plant traditionally used to treat malaria, smallpox, neuralgia, dysentery, diarrhea, and rheumatism in Northern Nigeria. There is no scientific evidence to support the antimalarial activity of this plant. This work aims to investigate the in vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (MPR) in mice, infected with NK65 chloroquine-sensitive Plasmodium berghei. The oral lethal doses and preliminary phytochemical screening of the extract were performed. The therapeutic, suppressive, and prophylactic models were used for the antiplasmodial activity at the doses of 250, 500, and 1000 mg/kg of the MPR extract. Chloroquine and artesunate were used as the positive control drugs, while distilled water was used for the negative control group. The antiplasmodial activity was determined by comparing the mean parasite clearance in the treated groups, to the negative control group. Also the effect of the extract on the blood packed-cell volume of mice (PCV) was determined. Results The LD50 of MPR was found to be < 5000 mg/kg. Glycosides, saponins, tannins, flavonoids, triterpenes and alkaloids were the phytochemicals identified in the extract. The extract of MPR produced a significant reduction in the mean parasitemia level compared to the negative control in the curative test: MPR 250 (68.31%, P < 0.001), MPR 500 (76.53%, P < 0.001), and MPR1000 (83.65%, P < 0.001). The extract prolonged the survival of infected mice (18.8 days), compared to the negative control (5.2 days). The extract produced significant chemosuppression compared to the negative control; MPR 250 (73.79%, P < 0.001), MPR 500 (81.33%, P < 0.001), and MPR 1000 (78.37%, P < 0.001). The extract produced significant chemoprophylaxis compared to the negative control; MPR 250 (68.5%, P < 0.001), MPR 500 (58.7%, P < 0.001), and MPR 1000 (84.77%, P < 0.001). The extract was found to have no significant effect on the blood PCV of the treated groups compared to the negative control. Conclusions The study showed that the MPR extract has significant antiplasmodial activity in mice at the doses tested, and could justify the traditional use of the plant in the treatment of malaria in Northern Nigeria.
abstractGer	Abstract Background Piliostigma reticulatum is a plant traditionally used to treat malaria, smallpox, neuralgia, dysentery, diarrhea, and rheumatism in Northern Nigeria. There is no scientific evidence to support the antimalarial activity of this plant. This work aims to investigate the in vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (MPR) in mice, infected with NK65 chloroquine-sensitive Plasmodium berghei. The oral lethal doses and preliminary phytochemical screening of the extract were performed. The therapeutic, suppressive, and prophylactic models were used for the antiplasmodial activity at the doses of 250, 500, and 1000 mg/kg of the MPR extract. Chloroquine and artesunate were used as the positive control drugs, while distilled water was used for the negative control group. The antiplasmodial activity was determined by comparing the mean parasite clearance in the treated groups, to the negative control group. Also the effect of the extract on the blood packed-cell volume of mice (PCV) was determined. Results The LD50 of MPR was found to be < 5000 mg/kg. Glycosides, saponins, tannins, flavonoids, triterpenes and alkaloids were the phytochemicals identified in the extract. The extract of MPR produced a significant reduction in the mean parasitemia level compared to the negative control in the curative test: MPR 250 (68.31%, P < 0.001), MPR 500 (76.53%, P < 0.001), and MPR1000 (83.65%, P < 0.001). The extract prolonged the survival of infected mice (18.8 days), compared to the negative control (5.2 days). The extract produced significant chemosuppression compared to the negative control; MPR 250 (73.79%, P < 0.001), MPR 500 (81.33%, P < 0.001), and MPR 1000 (78.37%, P < 0.001). The extract produced significant chemoprophylaxis compared to the negative control; MPR 250 (68.5%, P < 0.001), MPR 500 (58.7%, P < 0.001), and MPR 1000 (84.77%, P < 0.001). The extract was found to have no significant effect on the blood PCV of the treated groups compared to the negative control. Conclusions The study showed that the MPR extract has significant antiplasmodial activity in mice at the doses tested, and could justify the traditional use of the plant in the treatment of malaria in Northern Nigeria.
abstract_unstemmed	Abstract Background Piliostigma reticulatum is a plant traditionally used to treat malaria, smallpox, neuralgia, dysentery, diarrhea, and rheumatism in Northern Nigeria. There is no scientific evidence to support the antimalarial activity of this plant. This work aims to investigate the in vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (MPR) in mice, infected with NK65 chloroquine-sensitive Plasmodium berghei. The oral lethal doses and preliminary phytochemical screening of the extract were performed. The therapeutic, suppressive, and prophylactic models were used for the antiplasmodial activity at the doses of 250, 500, and 1000 mg/kg of the MPR extract. Chloroquine and artesunate were used as the positive control drugs, while distilled water was used for the negative control group. The antiplasmodial activity was determined by comparing the mean parasite clearance in the treated groups, to the negative control group. Also the effect of the extract on the blood packed-cell volume of mice (PCV) was determined. Results The LD50 of MPR was found to be < 5000 mg/kg. Glycosides, saponins, tannins, flavonoids, triterpenes and alkaloids were the phytochemicals identified in the extract. The extract of MPR produced a significant reduction in the mean parasitemia level compared to the negative control in the curative test: MPR 250 (68.31%, P < 0.001), MPR 500 (76.53%, P < 0.001), and MPR1000 (83.65%, P < 0.001). The extract prolonged the survival of infected mice (18.8 days), compared to the negative control (5.2 days). The extract produced significant chemosuppression compared to the negative control; MPR 250 (73.79%, P < 0.001), MPR 500 (81.33%, P < 0.001), and MPR 1000 (78.37%, P < 0.001). The extract produced significant chemoprophylaxis compared to the negative control; MPR 250 (68.5%, P < 0.001), MPR 500 (58.7%, P < 0.001), and MPR 1000 (84.77%, P < 0.001). The extract was found to have no significant effect on the blood PCV of the treated groups compared to the negative control. Conclusions The study showed that the MPR extract has significant antiplasmodial activity in mice at the doses tested, and could justify the traditional use of the plant in the treatment of malaria in Northern Nigeria.
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title_short	In vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (Dc.) Hochst (Fabaceae)
url	https://doi.org/10.1186/s42269-022-00910-0 https://doaj.org/article/c808ab845c21418e98128402757e9dff https://doaj.org/toc/2522-8307
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This work aims to investigate the in vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (MPR) in mice, infected with NK65 chloroquine-sensitive Plasmodium berghei. The oral lethal doses and preliminary phytochemical screening of the extract were performed. The therapeutic, suppressive, and prophylactic models were used for the antiplasmodial activity at the doses of 250, 500, and 1000 mg/kg of the MPR extract. Chloroquine and artesunate were used as the positive control drugs, while distilled water was used for the negative control group. The antiplasmodial activity was determined by comparing the mean parasite clearance in the treated groups, to the negative control group. Also the effect of the extract on the blood packed-cell volume of mice (PCV) was determined. Results The LD50 of MPR was found to be < 5000 mg/kg. Glycosides, saponins, tannins, flavonoids, triterpenes and alkaloids were the phytochemicals identified in the extract. 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In vivo antiplasmodial activity of the methanol leaf extract of Piliostigma reticulatum (Dc.) Hochst (Fabaceae)

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