Immunoprevalence to Six Waterborne Pathogens in Beachgoers at Boquerón Beach, Puerto Rico: Application of a Microsphere-Based Salivary Antibody Multiplex Immunoassay

Waterborne infectious diseases are a major public health concern worldwide. Few methods have been established that are capable of measuring human exposure to multiple waterborne pathogens simultaneously using non-invasive samples such as saliva. Most current methods measure exposure to only one path...
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Autor*in:	Swinburne A. J. Augustine [verfasserIn] Kaneatra J. Simmons [verfasserIn] Tarsha N. Eason [verfasserIn] Clarissa L. Curioso [verfasserIn] Shannon M. Griffin [verfasserIn] Timothy J. Wade [verfasserIn] Alfred Dufour [verfasserIn] G. Shay Fout [verfasserIn] Ann C. Grimm [verfasserIn] Kevin H. Oshima [verfasserIn] Elizabeth A. Sams [verfasserIn] Mary Jean See [verfasserIn] Larry J. Wymer [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	multiplex immunoassay salivary antibody saliva exposure bead-based

Übergeordnetes Werk:	In: Frontiers in Public Health - Frontiers Media S.A., 2013, 5(2017)
Übergeordnetes Werk:	volume:5 ; year:2017

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fpubh.2017.00084

Katalog-ID:	DOAJ031962912

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spelling	10.3389/fpubh.2017.00084 doi (DE-627)DOAJ031962912 (DE-599)DOAJ6498c8d73b344deeb089875d8a0d3b73 DE-627 ger DE-627 rakwb eng RA1-1270 Swinburne A. J. Augustine verfasserin aut Immunoprevalence to Six Waterborne Pathogens in Beachgoers at Boquerón Beach, Puerto Rico: Application of a Microsphere-Based Salivary Antibody Multiplex Immunoassay 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Waterborne infectious diseases are a major public health concern worldwide. Few methods have been established that are capable of measuring human exposure to multiple waterborne pathogens simultaneously using non-invasive samples such as saliva. Most current methods measure exposure to only one pathogen at a time, require large volumes of individual samples collected using invasive procedures, and are very labor intensive. In this article, we applied a multiplex bead-based immunoassay capable of measuring IgG antibody responses to six waterborne pathogens simultaneously in human saliva to estimate immunoprevalence in beachgoers at Boquerón Beach, Puerto Rico. Further, we present approaches for determining cutoff points to assess immunoprevalence to the pathogens in the assay. For the six pathogens studied, our results show that IgG antibodies against antigens from noroviruses GI.I and GII.4 were more prevalent (60 and 51.6%, respectively) than Helicobacter pylori (21.4%), hepatitis A virus (20.2%), Campylobacter jejuni (8.7%), and Toxoplasma gondii (8%) in the saliva of the study participants. The salivary antibody multiplex immunoassay can be used to examine immunoprevalence of specific pathogens in human populations. multiplex immunoassay salivary antibody saliva exposure bead-based Public aspects of medicine Kaneatra J. Simmons verfasserin aut Tarsha N. Eason verfasserin aut Clarissa L. Curioso verfasserin aut Shannon M. Griffin verfasserin aut Timothy J. Wade verfasserin aut Alfred Dufour verfasserin aut G. Shay Fout verfasserin aut Ann C. Grimm verfasserin aut Kevin H. Oshima verfasserin aut Elizabeth A. Sams verfasserin aut Mary Jean See verfasserin aut Larry J. Wymer verfasserin aut In Frontiers in Public Health Frontiers Media S.A., 2013 5(2017) (DE-627)742224589 (DE-600)2711781-9 22962565 nnns volume:5 year:2017 https://doi.org/10.3389/fpubh.2017.00084 kostenfrei https://doaj.org/article/6498c8d73b344deeb089875d8a0d3b73 kostenfrei http://journal.frontiersin.org/article/10.3389/fpubh.2017.00084/full kostenfrei https://doaj.org/toc/2296-2565 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2017
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allfieldsSound	10.3389/fpubh.2017.00084 doi (DE-627)DOAJ031962912 (DE-599)DOAJ6498c8d73b344deeb089875d8a0d3b73 DE-627 ger DE-627 rakwb eng RA1-1270 Swinburne A. J. Augustine verfasserin aut Immunoprevalence to Six Waterborne Pathogens in Beachgoers at Boquerón Beach, Puerto Rico: Application of a Microsphere-Based Salivary Antibody Multiplex Immunoassay 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Waterborne infectious diseases are a major public health concern worldwide. Few methods have been established that are capable of measuring human exposure to multiple waterborne pathogens simultaneously using non-invasive samples such as saliva. Most current methods measure exposure to only one pathogen at a time, require large volumes of individual samples collected using invasive procedures, and are very labor intensive. In this article, we applied a multiplex bead-based immunoassay capable of measuring IgG antibody responses to six waterborne pathogens simultaneously in human saliva to estimate immunoprevalence in beachgoers at Boquerón Beach, Puerto Rico. Further, we present approaches for determining cutoff points to assess immunoprevalence to the pathogens in the assay. For the six pathogens studied, our results show that IgG antibodies against antigens from noroviruses GI.I and GII.4 were more prevalent (60 and 51.6%, respectively) than Helicobacter pylori (21.4%), hepatitis A virus (20.2%), Campylobacter jejuni (8.7%), and Toxoplasma gondii (8%) in the saliva of the study participants. The salivary antibody multiplex immunoassay can be used to examine immunoprevalence of specific pathogens in human populations. multiplex immunoassay salivary antibody saliva exposure bead-based Public aspects of medicine Kaneatra J. Simmons verfasserin aut Tarsha N. Eason verfasserin aut Clarissa L. Curioso verfasserin aut Shannon M. Griffin verfasserin aut Timothy J. Wade verfasserin aut Alfred Dufour verfasserin aut G. Shay Fout verfasserin aut Ann C. Grimm verfasserin aut Kevin H. Oshima verfasserin aut Elizabeth A. Sams verfasserin aut Mary Jean See verfasserin aut Larry J. Wymer verfasserin aut In Frontiers in Public Health Frontiers Media S.A., 2013 5(2017) (DE-627)742224589 (DE-600)2711781-9 22962565 nnns volume:5 year:2017 https://doi.org/10.3389/fpubh.2017.00084 kostenfrei https://doaj.org/article/6498c8d73b344deeb089875d8a0d3b73 kostenfrei http://journal.frontiersin.org/article/10.3389/fpubh.2017.00084/full kostenfrei https://doaj.org/toc/2296-2565 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2017
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callnumber-first	R - Medicine
author	Swinburne A. J. Augustine
spellingShingle	Swinburne A. J. Augustine misc RA1-1270 misc multiplex misc immunoassay misc salivary antibody misc saliva misc exposure misc bead-based misc Public aspects of medicine Immunoprevalence to Six Waterborne Pathogens in Beachgoers at Boquerón Beach, Puerto Rico: Application of a Microsphere-Based Salivary Antibody Multiplex Immunoassay
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topic_title	RA1-1270 Immunoprevalence to Six Waterborne Pathogens in Beachgoers at Boquerón Beach, Puerto Rico: Application of a Microsphere-Based Salivary Antibody Multiplex Immunoassay multiplex immunoassay salivary antibody saliva exposure bead-based
topic	misc RA1-1270 misc multiplex misc immunoassay misc salivary antibody misc saliva misc exposure misc bead-based misc Public aspects of medicine
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title	Immunoprevalence to Six Waterborne Pathogens in Beachgoers at Boquerón Beach, Puerto Rico: Application of a Microsphere-Based Salivary Antibody Multiplex Immunoassay
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title_full	Immunoprevalence to Six Waterborne Pathogens in Beachgoers at Boquerón Beach, Puerto Rico: Application of a Microsphere-Based Salivary Antibody Multiplex Immunoassay
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author_browse	Swinburne A. J. Augustine Kaneatra J. Simmons Tarsha N. Eason Clarissa L. Curioso Shannon M. Griffin Timothy J. Wade Alfred Dufour G. Shay Fout Ann C. Grimm Kevin H. Oshima Elizabeth A. Sams Mary Jean See Larry J. Wymer
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title_sort	immunoprevalence to six waterborne pathogens in beachgoers at boquerón beach, puerto rico: application of a microsphere-based salivary antibody multiplex immunoassay
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title_auth	Immunoprevalence to Six Waterborne Pathogens in Beachgoers at Boquerón Beach, Puerto Rico: Application of a Microsphere-Based Salivary Antibody Multiplex Immunoassay
abstract	Waterborne infectious diseases are a major public health concern worldwide. Few methods have been established that are capable of measuring human exposure to multiple waterborne pathogens simultaneously using non-invasive samples such as saliva. Most current methods measure exposure to only one pathogen at a time, require large volumes of individual samples collected using invasive procedures, and are very labor intensive. In this article, we applied a multiplex bead-based immunoassay capable of measuring IgG antibody responses to six waterborne pathogens simultaneously in human saliva to estimate immunoprevalence in beachgoers at Boquerón Beach, Puerto Rico. Further, we present approaches for determining cutoff points to assess immunoprevalence to the pathogens in the assay. For the six pathogens studied, our results show that IgG antibodies against antigens from noroviruses GI.I and GII.4 were more prevalent (60 and 51.6%, respectively) than Helicobacter pylori (21.4%), hepatitis A virus (20.2%), Campylobacter jejuni (8.7%), and Toxoplasma gondii (8%) in the saliva of the study participants. The salivary antibody multiplex immunoassay can be used to examine immunoprevalence of specific pathogens in human populations.
abstractGer	Waterborne infectious diseases are a major public health concern worldwide. Few methods have been established that are capable of measuring human exposure to multiple waterborne pathogens simultaneously using non-invasive samples such as saliva. Most current methods measure exposure to only one pathogen at a time, require large volumes of individual samples collected using invasive procedures, and are very labor intensive. In this article, we applied a multiplex bead-based immunoassay capable of measuring IgG antibody responses to six waterborne pathogens simultaneously in human saliva to estimate immunoprevalence in beachgoers at Boquerón Beach, Puerto Rico. Further, we present approaches for determining cutoff points to assess immunoprevalence to the pathogens in the assay. For the six pathogens studied, our results show that IgG antibodies against antigens from noroviruses GI.I and GII.4 were more prevalent (60 and 51.6%, respectively) than Helicobacter pylori (21.4%), hepatitis A virus (20.2%), Campylobacter jejuni (8.7%), and Toxoplasma gondii (8%) in the saliva of the study participants. The salivary antibody multiplex immunoassay can be used to examine immunoprevalence of specific pathogens in human populations.
abstract_unstemmed	Waterborne infectious diseases are a major public health concern worldwide. Few methods have been established that are capable of measuring human exposure to multiple waterborne pathogens simultaneously using non-invasive samples such as saliva. Most current methods measure exposure to only one pathogen at a time, require large volumes of individual samples collected using invasive procedures, and are very labor intensive. In this article, we applied a multiplex bead-based immunoassay capable of measuring IgG antibody responses to six waterborne pathogens simultaneously in human saliva to estimate immunoprevalence in beachgoers at Boquerón Beach, Puerto Rico. Further, we present approaches for determining cutoff points to assess immunoprevalence to the pathogens in the assay. For the six pathogens studied, our results show that IgG antibodies against antigens from noroviruses GI.I and GII.4 were more prevalent (60 and 51.6%, respectively) than Helicobacter pylori (21.4%), hepatitis A virus (20.2%), Campylobacter jejuni (8.7%), and Toxoplasma gondii (8%) in the saliva of the study participants. The salivary antibody multiplex immunoassay can be used to examine immunoprevalence of specific pathogens in human populations.
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title_short	Immunoprevalence to Six Waterborne Pathogens in Beachgoers at Boquerón Beach, Puerto Rico: Application of a Microsphere-Based Salivary Antibody Multiplex Immunoassay
url	https://doi.org/10.3389/fpubh.2017.00084 https://doaj.org/article/6498c8d73b344deeb089875d8a0d3b73 http://journal.frontiersin.org/article/10.3389/fpubh.2017.00084/full https://doaj.org/toc/2296-2565
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author2	Kaneatra J. Simmons Tarsha N. Eason Clarissa L. Curioso Shannon M. Griffin Timothy J. Wade Alfred Dufour G. Shay Fout Ann C. Grimm Kevin H. Oshima Elizabeth A. Sams Mary Jean See Larry J. Wymer
author2Str	Kaneatra J. Simmons Tarsha N. Eason Clarissa L. Curioso Shannon M. Griffin Timothy J. Wade Alfred Dufour G. Shay Fout Ann C. Grimm Kevin H. Oshima Elizabeth A. Sams Mary Jean See Larry J. Wymer
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up_date	2024-07-03T23:19:36.295Z
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For the six pathogens studied, our results show that IgG antibodies against antigens from noroviruses GI.I and GII.4 were more prevalent (60 and 51.6%, respectively) than Helicobacter pylori (21.4%), hepatitis A virus (20.2%), Campylobacter jejuni (8.7%), and Toxoplasma gondii (8%) in the saliva of the study participants. 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Simmons</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Tarsha N. Eason</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Clarissa L. Curioso</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Shannon M. Griffin</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Timothy J. 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Immunoprevalence to Six Waterborne Pathogens in Beachgoers at Boquerón Beach, Puerto Rico: Application of a Microsphere-Based Salivary Antibody Multiplex Immunoassay

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