Differential Degradation and Detoxification of an Aromatic Pollutant by Two Different Peroxidases

Enzymatic degradation of organic pollutants is a new and promising remediation approach. Peroxidases are one of the most commonly used classes of enzymes to degrade organic pollutants. However, it is generally assumed that all peroxidases behave similarly and produce similar degradation products. In...
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Autor*in:	Aysha Hamad Alneyadi [verfasserIn] Iltaf Shah [verfasserIn] Synan F. AbuQamar [verfasserIn] Syed Salman Ashraf [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	organic pollutants enzymatic remediation Sulforhodamine B soybean peroxidase chloroperoxidase

Übergeordnetes Werk:	In: Biomolecules - MDPI AG, 2013, 7(2017), 1, p 31
Übergeordnetes Werk:	volume:7 ; year:2017 ; number:1, p 31

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/biom7010031

Katalog-ID:	DOAJ032023618

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language	English
source	In Biomolecules 7(2017), 1, p 31 volume:7 year:2017 number:1, p 31
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topic_facet	organic pollutants enzymatic remediation Sulforhodamine B soybean peroxidase chloroperoxidase Microbiology
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callnumber-first	Q - Science
author	Aysha Hamad Alneyadi
spellingShingle	Aysha Hamad Alneyadi misc QR1-502 misc organic pollutants misc enzymatic remediation misc Sulforhodamine B misc soybean peroxidase misc chloroperoxidase misc Microbiology Differential Degradation and Detoxification of an Aromatic Pollutant by Two Different Peroxidases
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topic_title	QR1-502 Differential Degradation and Detoxification of an Aromatic Pollutant by Two Different Peroxidases organic pollutants enzymatic remediation Sulforhodamine B soybean peroxidase chloroperoxidase
topic	misc QR1-502 misc organic pollutants misc enzymatic remediation misc Sulforhodamine B misc soybean peroxidase misc chloroperoxidase misc Microbiology
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title	Differential Degradation and Detoxification of an Aromatic Pollutant by Two Different Peroxidases
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title_sort	differential degradation and detoxification of an aromatic pollutant by two different peroxidases
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title_auth	Differential Degradation and Detoxification of an Aromatic Pollutant by Two Different Peroxidases
abstract	Enzymatic degradation of organic pollutants is a new and promising remediation approach. Peroxidases are one of the most commonly used classes of enzymes to degrade organic pollutants. However, it is generally assumed that all peroxidases behave similarly and produce similar degradation products. In this study, we conducted detailed studies of the degradation of a model aromatic pollutant, Sulforhodamine B dye (SRB dye), using two peroxidases—soybean peroxidase (SBP) and chloroperoxidase (CPO). Our results show that these two related enzymes had different optimum conditions (pH, temperature, H2O2 concentration, etc.) for efficiently degrading SRB dye. High-performance liquid chromatography and liquid chromatography –mass spectrometry analyses confirmed that both SBP and CPO transformed the SRB dye into low molecular weight intermediates. While most of the intermediates produced by the two enzymes were the same, the CPO treatment produced at least one different intermediate. Furthermore, toxicological evaluation using lettuce (Lactuca sativa) seeds demonstrated that the SBP-based treatment was able to eliminate the phytotoxicity of SRB dye, but the CPO-based treatment did not. Our results show, for the first time, that while both of these related enzymes can be used to efficiently degrade organic pollutants, they have different optimum reaction conditions and may not be equally efficient in detoxification of organic pollutants.
abstractGer	Enzymatic degradation of organic pollutants is a new and promising remediation approach. Peroxidases are one of the most commonly used classes of enzymes to degrade organic pollutants. However, it is generally assumed that all peroxidases behave similarly and produce similar degradation products. In this study, we conducted detailed studies of the degradation of a model aromatic pollutant, Sulforhodamine B dye (SRB dye), using two peroxidases—soybean peroxidase (SBP) and chloroperoxidase (CPO). Our results show that these two related enzymes had different optimum conditions (pH, temperature, H2O2 concentration, etc.) for efficiently degrading SRB dye. High-performance liquid chromatography and liquid chromatography –mass spectrometry analyses confirmed that both SBP and CPO transformed the SRB dye into low molecular weight intermediates. While most of the intermediates produced by the two enzymes were the same, the CPO treatment produced at least one different intermediate. Furthermore, toxicological evaluation using lettuce (Lactuca sativa) seeds demonstrated that the SBP-based treatment was able to eliminate the phytotoxicity of SRB dye, but the CPO-based treatment did not. Our results show, for the first time, that while both of these related enzymes can be used to efficiently degrade organic pollutants, they have different optimum reaction conditions and may not be equally efficient in detoxification of organic pollutants.
abstract_unstemmed	Enzymatic degradation of organic pollutants is a new and promising remediation approach. Peroxidases are one of the most commonly used classes of enzymes to degrade organic pollutants. However, it is generally assumed that all peroxidases behave similarly and produce similar degradation products. In this study, we conducted detailed studies of the degradation of a model aromatic pollutant, Sulforhodamine B dye (SRB dye), using two peroxidases—soybean peroxidase (SBP) and chloroperoxidase (CPO). Our results show that these two related enzymes had different optimum conditions (pH, temperature, H2O2 concentration, etc.) for efficiently degrading SRB dye. High-performance liquid chromatography and liquid chromatography –mass spectrometry analyses confirmed that both SBP and CPO transformed the SRB dye into low molecular weight intermediates. While most of the intermediates produced by the two enzymes were the same, the CPO treatment produced at least one different intermediate. Furthermore, toxicological evaluation using lettuce (Lactuca sativa) seeds demonstrated that the SBP-based treatment was able to eliminate the phytotoxicity of SRB dye, but the CPO-based treatment did not. Our results show, for the first time, that while both of these related enzymes can be used to efficiently degrade organic pollutants, they have different optimum reaction conditions and may not be equally efficient in detoxification of organic pollutants.
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title_short	Differential Degradation and Detoxification of an Aromatic Pollutant by Two Different Peroxidases
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Differential Degradation and Detoxification of an Aromatic Pollutant by Two Different Peroxidases

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