VITRIFICATION, NEW METHOD OF EMBRYO CRYOPRESERVATION: MARIBOR EXPERIENCE

Background. Vitrification is a new, simpler and more rational way of cryopreservation of redundant embryos in in vitro fertilisation. The purpose of this study was to introduce vitrification of blastocysts in clinical practice and compared two commercial cryoprotectants. Methods. We started vitrifying blastocysts in 2008. Two commercial vitrification protocols were used, Vitrification Cooling/Warming (MediCult, Denmark) – (MC), and Vitrification Freeze/Thaw (IrvineScientific, ZDA) – (IS). IS medium includes two cryoprotectants: dimethylsulfoxide (DMSO) and ethylene glycol, meanwhile MC medium contains only ethylene glycol. Among 1030 in vitro fertilizated cycles, we preserved redundant blastocysts in 404 (39.2 %) cycles in liquid nitrogen. At the begining MC vitrification protocol was used in 253 cycles and later IS protocol in 151 cycles. 127 transfers (248 devitrified blastocysts) were performed using MC protocol and 71 transfers (124 blastocysts) using IS protocol. We compared the survival rate of blastocysts, rate of clinical and ongoing pregnancies and miscarriages. Cycles with at least one optimal blastocyst were evaluated separately. Results. Survival rate of vitrified blastocysts by IS protocol was statistically significantly higher than survival rate by MC protocol (88.7 % or 77 %; P = 0.0103). Implantation rate of devitrified blastocysts by IS and MC protocol are comparable (22.7 % or 17.8 %). The rate of miscarriages does not statistically differ between the protocols (18.2 % or 16.7 %). There are no statistically significant differences between IS and MC protocols in clinical (31.8 % or 24 %) and ongoing pregnancies (26.1 % or 20 %). Pregnancy rate in cycles with at least one optimal devitrified blastocyst is 45.7 % by IS protocol and 36.4 % by MC protocol. In the same cycles, the rate of miscarriages is 5.7 % by IS and 4.5 % by MC protocol. Conclusions. Higher values in all parameters were by IS media, even though the differences were not statistically significant because of low number of cycles. The only statistically significant difference between protocols was in survival rate in favor of IS protocol. The study continues. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Petra Roglič [verfasserIn] Martin Ivec [verfasserIn] Borut Kovačič [verfasserIn] Veljko Vlaisavljević [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Slowenisch

Erschienen:	2018

Schlagwörter:	blastociste vitrifikacija stopnja preživetja vgnezditev zanositev

Übergeordnetes Werk:	In: Zdravniški Vestnik - Slovenian Medical Association, 2012, 78(2018), 0
Übergeordnetes Werk:	volume:78 ; year:2018 ; number:0

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.6016/ZdravVestn.2786

Katalog-ID:	DOAJ032030320

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520			\|a Background. Vitrification is a new, simpler and more rational way of cryopreservation of redundant embryos in in vitro fertilisation. The purpose of this study was to introduce vitrification of blastocysts in clinical practice and compared two commercial cryoprotectants. Methods. We started vitrifying blastocysts in 2008. Two commercial vitrification protocols were used, Vitrification Cooling/Warming (MediCult, Denmark) – (MC), and Vitrification Freeze/Thaw (IrvineScientific, ZDA) – (IS). IS medium includes two cryoprotectants: dimethylsulfoxide (DMSO) and ethylene glycol, meanwhile MC medium contains only ethylene glycol. Among 1030 in vitro fertilizated cycles, we preserved redundant blastocysts in 404 (39.2 %) cycles in liquid nitrogen. At the begining MC vitrification protocol was used in 253 cycles and later IS protocol in 151 cycles. 127 transfers (248 devitrified blastocysts) were performed using MC protocol and 71 transfers (124 blastocysts) using IS protocol. We compared the survival rate of blastocysts, rate of clinical and ongoing pregnancies and miscarriages. Cycles with at least one optimal blastocyst were evaluated separately. Results. Survival rate of vitrified blastocysts by IS protocol was statistically significantly higher than survival rate by MC protocol (88.7 % or 77 %; P = 0.0103). Implantation rate of devitrified blastocysts by IS and MC protocol are comparable (22.7 % or 17.8 %). The rate of miscarriages does not statistically differ between the protocols (18.2 % or 16.7 %). There are no statistically significant differences between IS and MC protocols in clinical (31.8 % or 24 %) and ongoing pregnancies (26.1 % or 20 %). Pregnancy rate in cycles with at least one optimal devitrified blastocyst is 45.7 % by IS protocol and 36.4 % by MC protocol. In the same cycles, the rate of miscarriages is 5.7 % by IS and 4.5 % by MC protocol. Conclusions. Higher values in all parameters were by IS media, even though the differences were not statistically significant because of low number of cycles. The only statistically significant difference between protocols was in survival rate in favor of IS protocol. The study continues.
650		4	\|a blastociste
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912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
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912			\|a GBV_ILN_65
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912			\|a GBV_ILN_206
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author_variant	p r pr m i mi b k bk v v vv
matchkey_str	article:15810224:2018----::irfctonwehdfmrororsrai
hierarchy_sort_str	2018
publishDate	2018
allfields	10.6016/ZdravVestn.2786 doi (DE-627)DOAJ032030320 (DE-599)DOAJb79e5d2d4dc8466c982d5569b0028300 DE-627 ger DE-627 rakwb eng slv Petra Roglič verfasserin aut VITRIFICATION, NEW METHOD OF EMBRYO CRYOPRESERVATION: MARIBOR EXPERIENCE 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background. Vitrification is a new, simpler and more rational way of cryopreservation of redundant embryos in in vitro fertilisation. The purpose of this study was to introduce vitrification of blastocysts in clinical practice and compared two commercial cryoprotectants. Methods. We started vitrifying blastocysts in 2008. Two commercial vitrification protocols were used, Vitrification Cooling/Warming (MediCult, Denmark) – (MC), and Vitrification Freeze/Thaw (IrvineScientific, ZDA) – (IS). IS medium includes two cryoprotectants: dimethylsulfoxide (DMSO) and ethylene glycol, meanwhile MC medium contains only ethylene glycol. Among 1030 in vitro fertilizated cycles, we preserved redundant blastocysts in 404 (39.2 %) cycles in liquid nitrogen. At the begining MC vitrification protocol was used in 253 cycles and later IS protocol in 151 cycles. 127 transfers (248 devitrified blastocysts) were performed using MC protocol and 71 transfers (124 blastocysts) using IS protocol. We compared the survival rate of blastocysts, rate of clinical and ongoing pregnancies and miscarriages. Cycles with at least one optimal blastocyst were evaluated separately. Results. Survival rate of vitrified blastocysts by IS protocol was statistically significantly higher than survival rate by MC protocol (88.7 % or 77 %; P = 0.0103). Implantation rate of devitrified blastocysts by IS and MC protocol are comparable (22.7 % or 17.8 %). The rate of miscarriages does not statistically differ between the protocols (18.2 % or 16.7 %). There are no statistically significant differences between IS and MC protocols in clinical (31.8 % or 24 %) and ongoing pregnancies (26.1 % or 20 %). Pregnancy rate in cycles with at least one optimal devitrified blastocyst is 45.7 % by IS protocol and 36.4 % by MC protocol. In the same cycles, the rate of miscarriages is 5.7 % by IS and 4.5 % by MC protocol. Conclusions. Higher values in all parameters were by IS media, even though the differences were not statistically significant because of low number of cycles. The only statistically significant difference between protocols was in survival rate in favor of IS protocol. The study continues. blastociste vitrifikacija stopnja preživetja vgnezditev zanositev Medicine R Martin Ivec verfasserin aut Borut Kovačič verfasserin aut Veljko Vlaisavljević verfasserin aut In Zdravniški Vestnik Slovenian Medical Association, 2012 78(2018), 0 (DE-627)603492134 (DE-600)2502309-3 15810224 nnns volume:78 year:2018 number:0 https://doi.org/10.6016/ZdravVestn.2786 kostenfrei https://doaj.org/article/b79e5d2d4dc8466c982d5569b0028300 kostenfrei http://vestnik.szd.si/index.php/ZdravVest/article/view/2786 kostenfrei https://doaj.org/toc/1318-0347 Journal toc kostenfrei https://doaj.org/toc/1581-0224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 78 2018 0
spelling	10.6016/ZdravVestn.2786 doi (DE-627)DOAJ032030320 (DE-599)DOAJb79e5d2d4dc8466c982d5569b0028300 DE-627 ger DE-627 rakwb eng slv Petra Roglič verfasserin aut VITRIFICATION, NEW METHOD OF EMBRYO CRYOPRESERVATION: MARIBOR EXPERIENCE 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background. Vitrification is a new, simpler and more rational way of cryopreservation of redundant embryos in in vitro fertilisation. The purpose of this study was to introduce vitrification of blastocysts in clinical practice and compared two commercial cryoprotectants. Methods. We started vitrifying blastocysts in 2008. Two commercial vitrification protocols were used, Vitrification Cooling/Warming (MediCult, Denmark) – (MC), and Vitrification Freeze/Thaw (IrvineScientific, ZDA) – (IS). IS medium includes two cryoprotectants: dimethylsulfoxide (DMSO) and ethylene glycol, meanwhile MC medium contains only ethylene glycol. Among 1030 in vitro fertilizated cycles, we preserved redundant blastocysts in 404 (39.2 %) cycles in liquid nitrogen. At the begining MC vitrification protocol was used in 253 cycles and later IS protocol in 151 cycles. 127 transfers (248 devitrified blastocysts) were performed using MC protocol and 71 transfers (124 blastocysts) using IS protocol. We compared the survival rate of blastocysts, rate of clinical and ongoing pregnancies and miscarriages. Cycles with at least one optimal blastocyst were evaluated separately. Results. Survival rate of vitrified blastocysts by IS protocol was statistically significantly higher than survival rate by MC protocol (88.7 % or 77 %; P = 0.0103). Implantation rate of devitrified blastocysts by IS and MC protocol are comparable (22.7 % or 17.8 %). The rate of miscarriages does not statistically differ between the protocols (18.2 % or 16.7 %). There are no statistically significant differences between IS and MC protocols in clinical (31.8 % or 24 %) and ongoing pregnancies (26.1 % or 20 %). Pregnancy rate in cycles with at least one optimal devitrified blastocyst is 45.7 % by IS protocol and 36.4 % by MC protocol. In the same cycles, the rate of miscarriages is 5.7 % by IS and 4.5 % by MC protocol. Conclusions. Higher values in all parameters were by IS media, even though the differences were not statistically significant because of low number of cycles. The only statistically significant difference between protocols was in survival rate in favor of IS protocol. The study continues. blastociste vitrifikacija stopnja preživetja vgnezditev zanositev Medicine R Martin Ivec verfasserin aut Borut Kovačič verfasserin aut Veljko Vlaisavljević verfasserin aut In Zdravniški Vestnik Slovenian Medical Association, 2012 78(2018), 0 (DE-627)603492134 (DE-600)2502309-3 15810224 nnns volume:78 year:2018 number:0 https://doi.org/10.6016/ZdravVestn.2786 kostenfrei https://doaj.org/article/b79e5d2d4dc8466c982d5569b0028300 kostenfrei http://vestnik.szd.si/index.php/ZdravVest/article/view/2786 kostenfrei https://doaj.org/toc/1318-0347 Journal toc kostenfrei https://doaj.org/toc/1581-0224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 78 2018 0
allfields_unstemmed	10.6016/ZdravVestn.2786 doi (DE-627)DOAJ032030320 (DE-599)DOAJb79e5d2d4dc8466c982d5569b0028300 DE-627 ger DE-627 rakwb eng slv Petra Roglič verfasserin aut VITRIFICATION, NEW METHOD OF EMBRYO CRYOPRESERVATION: MARIBOR EXPERIENCE 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background. Vitrification is a new, simpler and more rational way of cryopreservation of redundant embryos in in vitro fertilisation. The purpose of this study was to introduce vitrification of blastocysts in clinical practice and compared two commercial cryoprotectants. Methods. We started vitrifying blastocysts in 2008. Two commercial vitrification protocols were used, Vitrification Cooling/Warming (MediCult, Denmark) – (MC), and Vitrification Freeze/Thaw (IrvineScientific, ZDA) – (IS). IS medium includes two cryoprotectants: dimethylsulfoxide (DMSO) and ethylene glycol, meanwhile MC medium contains only ethylene glycol. Among 1030 in vitro fertilizated cycles, we preserved redundant blastocysts in 404 (39.2 %) cycles in liquid nitrogen. At the begining MC vitrification protocol was used in 253 cycles and later IS protocol in 151 cycles. 127 transfers (248 devitrified blastocysts) were performed using MC protocol and 71 transfers (124 blastocysts) using IS protocol. We compared the survival rate of blastocysts, rate of clinical and ongoing pregnancies and miscarriages. Cycles with at least one optimal blastocyst were evaluated separately. Results. Survival rate of vitrified blastocysts by IS protocol was statistically significantly higher than survival rate by MC protocol (88.7 % or 77 %; P = 0.0103). Implantation rate of devitrified blastocysts by IS and MC protocol are comparable (22.7 % or 17.8 %). The rate of miscarriages does not statistically differ between the protocols (18.2 % or 16.7 %). There are no statistically significant differences between IS and MC protocols in clinical (31.8 % or 24 %) and ongoing pregnancies (26.1 % or 20 %). Pregnancy rate in cycles with at least one optimal devitrified blastocyst is 45.7 % by IS protocol and 36.4 % by MC protocol. In the same cycles, the rate of miscarriages is 5.7 % by IS and 4.5 % by MC protocol. Conclusions. Higher values in all parameters were by IS media, even though the differences were not statistically significant because of low number of cycles. The only statistically significant difference between protocols was in survival rate in favor of IS protocol. The study continues. blastociste vitrifikacija stopnja preživetja vgnezditev zanositev Medicine R Martin Ivec verfasserin aut Borut Kovačič verfasserin aut Veljko Vlaisavljević verfasserin aut In Zdravniški Vestnik Slovenian Medical Association, 2012 78(2018), 0 (DE-627)603492134 (DE-600)2502309-3 15810224 nnns volume:78 year:2018 number:0 https://doi.org/10.6016/ZdravVestn.2786 kostenfrei https://doaj.org/article/b79e5d2d4dc8466c982d5569b0028300 kostenfrei http://vestnik.szd.si/index.php/ZdravVest/article/view/2786 kostenfrei https://doaj.org/toc/1318-0347 Journal toc kostenfrei https://doaj.org/toc/1581-0224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 78 2018 0
allfieldsGer	10.6016/ZdravVestn.2786 doi (DE-627)DOAJ032030320 (DE-599)DOAJb79e5d2d4dc8466c982d5569b0028300 DE-627 ger DE-627 rakwb eng slv Petra Roglič verfasserin aut VITRIFICATION, NEW METHOD OF EMBRYO CRYOPRESERVATION: MARIBOR EXPERIENCE 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background. Vitrification is a new, simpler and more rational way of cryopreservation of redundant embryos in in vitro fertilisation. The purpose of this study was to introduce vitrification of blastocysts in clinical practice and compared two commercial cryoprotectants. Methods. We started vitrifying blastocysts in 2008. Two commercial vitrification protocols were used, Vitrification Cooling/Warming (MediCult, Denmark) – (MC), and Vitrification Freeze/Thaw (IrvineScientific, ZDA) – (IS). IS medium includes two cryoprotectants: dimethylsulfoxide (DMSO) and ethylene glycol, meanwhile MC medium contains only ethylene glycol. Among 1030 in vitro fertilizated cycles, we preserved redundant blastocysts in 404 (39.2 %) cycles in liquid nitrogen. At the begining MC vitrification protocol was used in 253 cycles and later IS protocol in 151 cycles. 127 transfers (248 devitrified blastocysts) were performed using MC protocol and 71 transfers (124 blastocysts) using IS protocol. We compared the survival rate of blastocysts, rate of clinical and ongoing pregnancies and miscarriages. Cycles with at least one optimal blastocyst were evaluated separately. Results. Survival rate of vitrified blastocysts by IS protocol was statistically significantly higher than survival rate by MC protocol (88.7 % or 77 %; P = 0.0103). Implantation rate of devitrified blastocysts by IS and MC protocol are comparable (22.7 % or 17.8 %). The rate of miscarriages does not statistically differ between the protocols (18.2 % or 16.7 %). There are no statistically significant differences between IS and MC protocols in clinical (31.8 % or 24 %) and ongoing pregnancies (26.1 % or 20 %). Pregnancy rate in cycles with at least one optimal devitrified blastocyst is 45.7 % by IS protocol and 36.4 % by MC protocol. In the same cycles, the rate of miscarriages is 5.7 % by IS and 4.5 % by MC protocol. Conclusions. Higher values in all parameters were by IS media, even though the differences were not statistically significant because of low number of cycles. The only statistically significant difference between protocols was in survival rate in favor of IS protocol. The study continues. blastociste vitrifikacija stopnja preživetja vgnezditev zanositev Medicine R Martin Ivec verfasserin aut Borut Kovačič verfasserin aut Veljko Vlaisavljević verfasserin aut In Zdravniški Vestnik Slovenian Medical Association, 2012 78(2018), 0 (DE-627)603492134 (DE-600)2502309-3 15810224 nnns volume:78 year:2018 number:0 https://doi.org/10.6016/ZdravVestn.2786 kostenfrei https://doaj.org/article/b79e5d2d4dc8466c982d5569b0028300 kostenfrei http://vestnik.szd.si/index.php/ZdravVest/article/view/2786 kostenfrei https://doaj.org/toc/1318-0347 Journal toc kostenfrei https://doaj.org/toc/1581-0224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 78 2018 0
allfieldsSound	10.6016/ZdravVestn.2786 doi (DE-627)DOAJ032030320 (DE-599)DOAJb79e5d2d4dc8466c982d5569b0028300 DE-627 ger DE-627 rakwb eng slv Petra Roglič verfasserin aut VITRIFICATION, NEW METHOD OF EMBRYO CRYOPRESERVATION: MARIBOR EXPERIENCE 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background. Vitrification is a new, simpler and more rational way of cryopreservation of redundant embryos in in vitro fertilisation. The purpose of this study was to introduce vitrification of blastocysts in clinical practice and compared two commercial cryoprotectants. Methods. We started vitrifying blastocysts in 2008. Two commercial vitrification protocols were used, Vitrification Cooling/Warming (MediCult, Denmark) – (MC), and Vitrification Freeze/Thaw (IrvineScientific, ZDA) – (IS). IS medium includes two cryoprotectants: dimethylsulfoxide (DMSO) and ethylene glycol, meanwhile MC medium contains only ethylene glycol. Among 1030 in vitro fertilizated cycles, we preserved redundant blastocysts in 404 (39.2 %) cycles in liquid nitrogen. At the begining MC vitrification protocol was used in 253 cycles and later IS protocol in 151 cycles. 127 transfers (248 devitrified blastocysts) were performed using MC protocol and 71 transfers (124 blastocysts) using IS protocol. We compared the survival rate of blastocysts, rate of clinical and ongoing pregnancies and miscarriages. Cycles with at least one optimal blastocyst were evaluated separately. Results. Survival rate of vitrified blastocysts by IS protocol was statistically significantly higher than survival rate by MC protocol (88.7 % or 77 %; P = 0.0103). Implantation rate of devitrified blastocysts by IS and MC protocol are comparable (22.7 % or 17.8 %). The rate of miscarriages does not statistically differ between the protocols (18.2 % or 16.7 %). There are no statistically significant differences between IS and MC protocols in clinical (31.8 % or 24 %) and ongoing pregnancies (26.1 % or 20 %). Pregnancy rate in cycles with at least one optimal devitrified blastocyst is 45.7 % by IS protocol and 36.4 % by MC protocol. In the same cycles, the rate of miscarriages is 5.7 % by IS and 4.5 % by MC protocol. Conclusions. Higher values in all parameters were by IS media, even though the differences were not statistically significant because of low number of cycles. The only statistically significant difference between protocols was in survival rate in favor of IS protocol. The study continues. blastociste vitrifikacija stopnja preživetja vgnezditev zanositev Medicine R Martin Ivec verfasserin aut Borut Kovačič verfasserin aut Veljko Vlaisavljević verfasserin aut In Zdravniški Vestnik Slovenian Medical Association, 2012 78(2018), 0 (DE-627)603492134 (DE-600)2502309-3 15810224 nnns volume:78 year:2018 number:0 https://doi.org/10.6016/ZdravVestn.2786 kostenfrei https://doaj.org/article/b79e5d2d4dc8466c982d5569b0028300 kostenfrei http://vestnik.szd.si/index.php/ZdravVest/article/view/2786 kostenfrei https://doaj.org/toc/1318-0347 Journal toc kostenfrei https://doaj.org/toc/1581-0224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 78 2018 0
language	English Slovenian
source	In Zdravniški Vestnik 78(2018), 0 volume:78 year:2018 number:0
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topic_facet	blastociste vitrifikacija stopnja preživetja vgnezditev zanositev Medicine R
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authorswithroles_txt_mv	Petra Roglič @@aut@@ Martin Ivec @@aut@@ Borut Kovačič @@aut@@ Veljko Vlaisavljević @@aut@@
publishDateDaySort_date	2018-01-01T00:00:00Z
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Vitrification is a new, simpler and more rational way of cryopreservation of redundant embryos in in vitro fertilisation. The purpose of this study was to introduce vitrification of blastocysts in clinical practice and compared two commercial cryoprotectants. Methods. We started vitrifying blastocysts in 2008. Two commercial vitrification protocols were used, Vitrification Cooling/Warming (MediCult, Denmark) – (MC), and Vitrification Freeze/Thaw (IrvineScientific, ZDA) – (IS). IS medium includes two cryoprotectants: dimethylsulfoxide (DMSO) and ethylene glycol, meanwhile MC medium contains only ethylene glycol. Among 1030 in vitro fertilizated cycles, we preserved redundant blastocysts in 404 (39.2 %) cycles in liquid nitrogen. At the begining MC vitrification protocol was used in 253 cycles and later IS protocol in 151 cycles. 127 transfers (248 devitrified blastocysts) were performed using MC protocol and 71 transfers (124 blastocysts) using IS protocol. We compared the survival rate of blastocysts, rate of clinical and ongoing pregnancies and miscarriages. Cycles with at least one optimal blastocyst were evaluated separately. Results. Survival rate of vitrified blastocysts by IS protocol was statistically significantly higher than survival rate by MC protocol (88.7 % or 77 %; P = 0.0103). Implantation rate of devitrified blastocysts by IS and MC protocol are comparable (22.7 % or 17.8 %). The rate of miscarriages does not statistically differ between the protocols (18.2 % or 16.7 %). There are no statistically significant differences between IS and MC protocols in clinical (31.8 % or 24 %) and ongoing pregnancies (26.1 % or 20 %). Pregnancy rate in cycles with at least one optimal devitrified blastocyst is 45.7 % by IS protocol and 36.4 % by MC protocol. In the same cycles, the rate of miscarriages is 5.7 % by IS and 4.5 % by MC protocol. Conclusions. Higher values in all parameters were by IS media, even though the differences were not statistically significant because of low number of cycles. The only statistically significant difference between protocols was in survival rate in favor of IS protocol. 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author	Petra Roglič
spellingShingle	Petra Roglič misc blastociste misc vitrifikacija misc stopnja preživetja misc vgnezditev misc zanositev misc Medicine misc R VITRIFICATION, NEW METHOD OF EMBRYO CRYOPRESERVATION: MARIBOR EXPERIENCE
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topic_title	VITRIFICATION, NEW METHOD OF EMBRYO CRYOPRESERVATION: MARIBOR EXPERIENCE blastociste vitrifikacija stopnja preživetja vgnezditev zanositev
topic	misc blastociste misc vitrifikacija misc stopnja preživetja misc vgnezditev misc zanositev misc Medicine misc R
topic_unstemmed	misc blastociste misc vitrifikacija misc stopnja preživetja misc vgnezditev misc zanositev misc Medicine misc R
topic_browse	misc blastociste misc vitrifikacija misc stopnja preživetja misc vgnezditev misc zanositev misc Medicine misc R
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title	VITRIFICATION, NEW METHOD OF EMBRYO CRYOPRESERVATION: MARIBOR EXPERIENCE
ctrlnum	(DE-627)DOAJ032030320 (DE-599)DOAJb79e5d2d4dc8466c982d5569b0028300
title_full	VITRIFICATION, NEW METHOD OF EMBRYO CRYOPRESERVATION: MARIBOR EXPERIENCE
author_sort	Petra Roglič
journal	Zdravniški Vestnik
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author_browse	Petra Roglič Martin Ivec Borut Kovačič Veljko Vlaisavljević
container_volume	78
format_se	Elektronische Aufsätze
author-letter	Petra Roglič
doi_str_mv	10.6016/ZdravVestn.2786
author2-role	verfasserin
title_sort	vitrification, new method of embryo cryopreservation: maribor experience
title_auth	VITRIFICATION, NEW METHOD OF EMBRYO CRYOPRESERVATION: MARIBOR EXPERIENCE
abstract	Background. Vitrification is a new, simpler and more rational way of cryopreservation of redundant embryos in in vitro fertilisation. The purpose of this study was to introduce vitrification of blastocysts in clinical practice and compared two commercial cryoprotectants. Methods. We started vitrifying blastocysts in 2008. Two commercial vitrification protocols were used, Vitrification Cooling/Warming (MediCult, Denmark) – (MC), and Vitrification Freeze/Thaw (IrvineScientific, ZDA) – (IS). IS medium includes two cryoprotectants: dimethylsulfoxide (DMSO) and ethylene glycol, meanwhile MC medium contains only ethylene glycol. Among 1030 in vitro fertilizated cycles, we preserved redundant blastocysts in 404 (39.2 %) cycles in liquid nitrogen. At the begining MC vitrification protocol was used in 253 cycles and later IS protocol in 151 cycles. 127 transfers (248 devitrified blastocysts) were performed using MC protocol and 71 transfers (124 blastocysts) using IS protocol. We compared the survival rate of blastocysts, rate of clinical and ongoing pregnancies and miscarriages. Cycles with at least one optimal blastocyst were evaluated separately. Results. Survival rate of vitrified blastocysts by IS protocol was statistically significantly higher than survival rate by MC protocol (88.7 % or 77 %; P = 0.0103). Implantation rate of devitrified blastocysts by IS and MC protocol are comparable (22.7 % or 17.8 %). The rate of miscarriages does not statistically differ between the protocols (18.2 % or 16.7 %). There are no statistically significant differences between IS and MC protocols in clinical (31.8 % or 24 %) and ongoing pregnancies (26.1 % or 20 %). Pregnancy rate in cycles with at least one optimal devitrified blastocyst is 45.7 % by IS protocol and 36.4 % by MC protocol. In the same cycles, the rate of miscarriages is 5.7 % by IS and 4.5 % by MC protocol. Conclusions. Higher values in all parameters were by IS media, even though the differences were not statistically significant because of low number of cycles. The only statistically significant difference between protocols was in survival rate in favor of IS protocol. The study continues.
abstractGer	Background. Vitrification is a new, simpler and more rational way of cryopreservation of redundant embryos in in vitro fertilisation. The purpose of this study was to introduce vitrification of blastocysts in clinical practice and compared two commercial cryoprotectants. Methods. We started vitrifying blastocysts in 2008. Two commercial vitrification protocols were used, Vitrification Cooling/Warming (MediCult, Denmark) – (MC), and Vitrification Freeze/Thaw (IrvineScientific, ZDA) – (IS). IS medium includes two cryoprotectants: dimethylsulfoxide (DMSO) and ethylene glycol, meanwhile MC medium contains only ethylene glycol. Among 1030 in vitro fertilizated cycles, we preserved redundant blastocysts in 404 (39.2 %) cycles in liquid nitrogen. At the begining MC vitrification protocol was used in 253 cycles and later IS protocol in 151 cycles. 127 transfers (248 devitrified blastocysts) were performed using MC protocol and 71 transfers (124 blastocysts) using IS protocol. We compared the survival rate of blastocysts, rate of clinical and ongoing pregnancies and miscarriages. Cycles with at least one optimal blastocyst were evaluated separately. Results. Survival rate of vitrified blastocysts by IS protocol was statistically significantly higher than survival rate by MC protocol (88.7 % or 77 %; P = 0.0103). Implantation rate of devitrified blastocysts by IS and MC protocol are comparable (22.7 % or 17.8 %). The rate of miscarriages does not statistically differ between the protocols (18.2 % or 16.7 %). There are no statistically significant differences between IS and MC protocols in clinical (31.8 % or 24 %) and ongoing pregnancies (26.1 % or 20 %). Pregnancy rate in cycles with at least one optimal devitrified blastocyst is 45.7 % by IS protocol and 36.4 % by MC protocol. In the same cycles, the rate of miscarriages is 5.7 % by IS and 4.5 % by MC protocol. Conclusions. Higher values in all parameters were by IS media, even though the differences were not statistically significant because of low number of cycles. The only statistically significant difference between protocols was in survival rate in favor of IS protocol. The study continues.
abstract_unstemmed	Background. Vitrification is a new, simpler and more rational way of cryopreservation of redundant embryos in in vitro fertilisation. The purpose of this study was to introduce vitrification of blastocysts in clinical practice and compared two commercial cryoprotectants. Methods. We started vitrifying blastocysts in 2008. Two commercial vitrification protocols were used, Vitrification Cooling/Warming (MediCult, Denmark) – (MC), and Vitrification Freeze/Thaw (IrvineScientific, ZDA) – (IS). IS medium includes two cryoprotectants: dimethylsulfoxide (DMSO) and ethylene glycol, meanwhile MC medium contains only ethylene glycol. Among 1030 in vitro fertilizated cycles, we preserved redundant blastocysts in 404 (39.2 %) cycles in liquid nitrogen. At the begining MC vitrification protocol was used in 253 cycles and later IS protocol in 151 cycles. 127 transfers (248 devitrified blastocysts) were performed using MC protocol and 71 transfers (124 blastocysts) using IS protocol. We compared the survival rate of blastocysts, rate of clinical and ongoing pregnancies and miscarriages. Cycles with at least one optimal blastocyst were evaluated separately. Results. Survival rate of vitrified blastocysts by IS protocol was statistically significantly higher than survival rate by MC protocol (88.7 % or 77 %; P = 0.0103). Implantation rate of devitrified blastocysts by IS and MC protocol are comparable (22.7 % or 17.8 %). The rate of miscarriages does not statistically differ between the protocols (18.2 % or 16.7 %). There are no statistically significant differences between IS and MC protocols in clinical (31.8 % or 24 %) and ongoing pregnancies (26.1 % or 20 %). Pregnancy rate in cycles with at least one optimal devitrified blastocyst is 45.7 % by IS protocol and 36.4 % by MC protocol. In the same cycles, the rate of miscarriages is 5.7 % by IS and 4.5 % by MC protocol. Conclusions. Higher values in all parameters were by IS media, even though the differences were not statistically significant because of low number of cycles. The only statistically significant difference between protocols was in survival rate in favor of IS protocol. The study continues.
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title_short	VITRIFICATION, NEW METHOD OF EMBRYO CRYOPRESERVATION: MARIBOR EXPERIENCE
url	https://doi.org/10.6016/ZdravVestn.2786 https://doaj.org/article/b79e5d2d4dc8466c982d5569b0028300 http://vestnik.szd.si/index.php/ZdravVest/article/view/2786 https://doaj.org/toc/1318-0347 https://doaj.org/toc/1581-0224
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author2	Martin Ivec Borut Kovačič Veljko Vlaisavljević
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up_date	2024-07-03T23:37:55.453Z
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