Vibrator and PI4KIIIα govern neuroblast polarity by anchoring non-muscle myosin II

A central feature of most stem cells is the ability to self-renew and undergo differentiation via asymmetric division. However, during asymmetric division the role of phosphatidylinositol (PI) lipids and their regulators is not well established. Here, we show that the sole type I PI transfer protein...
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Autor*in:	Chwee Tat Koe [verfasserIn] Ye Sing Tan [verfasserIn] Max Lönnfors [verfasserIn] Seong Kwon Hur [verfasserIn] Christine Siok Lan Low [verfasserIn] Yingjie Zhang [verfasserIn] Pakorn Kanchanawong [verfasserIn] Vytas A Bankaitis [verfasserIn] Hongyan Wang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	neuroblast Drosophila asymmetric division phosphatidylinositol lipids

Übergeordnetes Werk:	In: eLife - eLife Sciences Publications Ltd, 2013, 7(2018)
Übergeordnetes Werk:	volume:7 ; year:2018

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.7554/eLife.33555

Katalog-ID:	DOAJ032225733

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allfields	10.7554/eLife.33555 doi (DE-627)DOAJ032225733 (DE-599)DOAJf89c1270291546408dfdbcb38f7cbefd DE-627 ger DE-627 rakwb eng QH301-705.5 Chwee Tat Koe verfasserin aut Vibrator and PI4KIIIα govern neuroblast polarity by anchoring non-muscle myosin II 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A central feature of most stem cells is the ability to self-renew and undergo differentiation via asymmetric division. However, during asymmetric division the role of phosphatidylinositol (PI) lipids and their regulators is not well established. Here, we show that the sole type I PI transfer protein, Vibrator, controls asymmetric division of Drosophilaneural stem cells (NSCs) by physically anchoring myosin II regulatory light chain, Sqh, to the NSC cortex. Depletion of vib or disruption of its lipid binding and transfer activities disrupts NSC polarity. We propose that Vib stimulates PI4KIIIα to promote synthesis of a plasma membrane pool of phosphatidylinositol 4-phosphate [PI(4)P] that, in turn, binds and anchors myosin to the NSC cortex. Remarkably, Sqh also binds to PI(4)P in vitro and both Vib and Sqh mediate plasma membrane localization of PI(4)P in NSCs. Thus, reciprocal regulation between Myosin and PI(4)P likely governs asymmetric division of NSCs. neuroblast Drosophila asymmetric division phosphatidylinositol lipids Medicine R Science Q Biology (General) Ye Sing Tan verfasserin aut Max Lönnfors verfasserin aut Seong Kwon Hur verfasserin aut Christine Siok Lan Low verfasserin aut Yingjie Zhang verfasserin aut Pakorn Kanchanawong verfasserin aut Vytas A Bankaitis verfasserin aut Hongyan Wang verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 7(2018) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:7 year:2018 https://doi.org/10.7554/eLife.33555 kostenfrei https://doaj.org/article/f89c1270291546408dfdbcb38f7cbefd kostenfrei https://elifesciences.org/articles/33555 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2018
spelling	10.7554/eLife.33555 doi (DE-627)DOAJ032225733 (DE-599)DOAJf89c1270291546408dfdbcb38f7cbefd DE-627 ger DE-627 rakwb eng QH301-705.5 Chwee Tat Koe verfasserin aut Vibrator and PI4KIIIα govern neuroblast polarity by anchoring non-muscle myosin II 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A central feature of most stem cells is the ability to self-renew and undergo differentiation via asymmetric division. However, during asymmetric division the role of phosphatidylinositol (PI) lipids and their regulators is not well established. Here, we show that the sole type I PI transfer protein, Vibrator, controls asymmetric division of Drosophilaneural stem cells (NSCs) by physically anchoring myosin II regulatory light chain, Sqh, to the NSC cortex. Depletion of vib or disruption of its lipid binding and transfer activities disrupts NSC polarity. We propose that Vib stimulates PI4KIIIα to promote synthesis of a plasma membrane pool of phosphatidylinositol 4-phosphate [PI(4)P] that, in turn, binds and anchors myosin to the NSC cortex. Remarkably, Sqh also binds to PI(4)P in vitro and both Vib and Sqh mediate plasma membrane localization of PI(4)P in NSCs. Thus, reciprocal regulation between Myosin and PI(4)P likely governs asymmetric division of NSCs. neuroblast Drosophila asymmetric division phosphatidylinositol lipids Medicine R Science Q Biology (General) Ye Sing Tan verfasserin aut Max Lönnfors verfasserin aut Seong Kwon Hur verfasserin aut Christine Siok Lan Low verfasserin aut Yingjie Zhang verfasserin aut Pakorn Kanchanawong verfasserin aut Vytas A Bankaitis verfasserin aut Hongyan Wang verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 7(2018) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:7 year:2018 https://doi.org/10.7554/eLife.33555 kostenfrei https://doaj.org/article/f89c1270291546408dfdbcb38f7cbefd kostenfrei https://elifesciences.org/articles/33555 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2018
allfields_unstemmed	10.7554/eLife.33555 doi (DE-627)DOAJ032225733 (DE-599)DOAJf89c1270291546408dfdbcb38f7cbefd DE-627 ger DE-627 rakwb eng QH301-705.5 Chwee Tat Koe verfasserin aut Vibrator and PI4KIIIα govern neuroblast polarity by anchoring non-muscle myosin II 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A central feature of most stem cells is the ability to self-renew and undergo differentiation via asymmetric division. However, during asymmetric division the role of phosphatidylinositol (PI) lipids and their regulators is not well established. Here, we show that the sole type I PI transfer protein, Vibrator, controls asymmetric division of Drosophilaneural stem cells (NSCs) by physically anchoring myosin II regulatory light chain, Sqh, to the NSC cortex. Depletion of vib or disruption of its lipid binding and transfer activities disrupts NSC polarity. We propose that Vib stimulates PI4KIIIα to promote synthesis of a plasma membrane pool of phosphatidylinositol 4-phosphate [PI(4)P] that, in turn, binds and anchors myosin to the NSC cortex. Remarkably, Sqh also binds to PI(4)P in vitro and both Vib and Sqh mediate plasma membrane localization of PI(4)P in NSCs. Thus, reciprocal regulation between Myosin and PI(4)P likely governs asymmetric division of NSCs. neuroblast Drosophila asymmetric division phosphatidylinositol lipids Medicine R Science Q Biology (General) Ye Sing Tan verfasserin aut Max Lönnfors verfasserin aut Seong Kwon Hur verfasserin aut Christine Siok Lan Low verfasserin aut Yingjie Zhang verfasserin aut Pakorn Kanchanawong verfasserin aut Vytas A Bankaitis verfasserin aut Hongyan Wang verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 7(2018) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:7 year:2018 https://doi.org/10.7554/eLife.33555 kostenfrei https://doaj.org/article/f89c1270291546408dfdbcb38f7cbefd kostenfrei https://elifesciences.org/articles/33555 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2018
allfieldsGer	10.7554/eLife.33555 doi (DE-627)DOAJ032225733 (DE-599)DOAJf89c1270291546408dfdbcb38f7cbefd DE-627 ger DE-627 rakwb eng QH301-705.5 Chwee Tat Koe verfasserin aut Vibrator and PI4KIIIα govern neuroblast polarity by anchoring non-muscle myosin II 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A central feature of most stem cells is the ability to self-renew and undergo differentiation via asymmetric division. However, during asymmetric division the role of phosphatidylinositol (PI) lipids and their regulators is not well established. Here, we show that the sole type I PI transfer protein, Vibrator, controls asymmetric division of Drosophilaneural stem cells (NSCs) by physically anchoring myosin II regulatory light chain, Sqh, to the NSC cortex. Depletion of vib or disruption of its lipid binding and transfer activities disrupts NSC polarity. We propose that Vib stimulates PI4KIIIα to promote synthesis of a plasma membrane pool of phosphatidylinositol 4-phosphate [PI(4)P] that, in turn, binds and anchors myosin to the NSC cortex. Remarkably, Sqh also binds to PI(4)P in vitro and both Vib and Sqh mediate plasma membrane localization of PI(4)P in NSCs. Thus, reciprocal regulation between Myosin and PI(4)P likely governs asymmetric division of NSCs. neuroblast Drosophila asymmetric division phosphatidylinositol lipids Medicine R Science Q Biology (General) Ye Sing Tan verfasserin aut Max Lönnfors verfasserin aut Seong Kwon Hur verfasserin aut Christine Siok Lan Low verfasserin aut Yingjie Zhang verfasserin aut Pakorn Kanchanawong verfasserin aut Vytas A Bankaitis verfasserin aut Hongyan Wang verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 7(2018) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:7 year:2018 https://doi.org/10.7554/eLife.33555 kostenfrei https://doaj.org/article/f89c1270291546408dfdbcb38f7cbefd kostenfrei https://elifesciences.org/articles/33555 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2018
allfieldsSound	10.7554/eLife.33555 doi (DE-627)DOAJ032225733 (DE-599)DOAJf89c1270291546408dfdbcb38f7cbefd DE-627 ger DE-627 rakwb eng QH301-705.5 Chwee Tat Koe verfasserin aut Vibrator and PI4KIIIα govern neuroblast polarity by anchoring non-muscle myosin II 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A central feature of most stem cells is the ability to self-renew and undergo differentiation via asymmetric division. However, during asymmetric division the role of phosphatidylinositol (PI) lipids and their regulators is not well established. Here, we show that the sole type I PI transfer protein, Vibrator, controls asymmetric division of Drosophilaneural stem cells (NSCs) by physically anchoring myosin II regulatory light chain, Sqh, to the NSC cortex. Depletion of vib or disruption of its lipid binding and transfer activities disrupts NSC polarity. We propose that Vib stimulates PI4KIIIα to promote synthesis of a plasma membrane pool of phosphatidylinositol 4-phosphate [PI(4)P] that, in turn, binds and anchors myosin to the NSC cortex. Remarkably, Sqh also binds to PI(4)P in vitro and both Vib and Sqh mediate plasma membrane localization of PI(4)P in NSCs. Thus, reciprocal regulation between Myosin and PI(4)P likely governs asymmetric division of NSCs. neuroblast Drosophila asymmetric division phosphatidylinositol lipids Medicine R Science Q Biology (General) Ye Sing Tan verfasserin aut Max Lönnfors verfasserin aut Seong Kwon Hur verfasserin aut Christine Siok Lan Low verfasserin aut Yingjie Zhang verfasserin aut Pakorn Kanchanawong verfasserin aut Vytas A Bankaitis verfasserin aut Hongyan Wang verfasserin aut In eLife eLife Sciences Publications Ltd, 2013 7(2018) (DE-627)728518384 (DE-600)2687154-3 2050084X nnns volume:7 year:2018 https://doi.org/10.7554/eLife.33555 kostenfrei https://doaj.org/article/f89c1270291546408dfdbcb38f7cbefd kostenfrei https://elifesciences.org/articles/33555 kostenfrei https://doaj.org/toc/2050-084X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2018
language	English
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institution	findex.gbv.de
topic_facet	neuroblast Drosophila asymmetric division phosphatidylinositol lipids Medicine R Science Q Biology (General)
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container_title	eLife
authorswithroles_txt_mv	Chwee Tat Koe @@aut@@ Ye Sing Tan @@aut@@ Max Lönnfors @@aut@@ Seong Kwon Hur @@aut@@ Christine Siok Lan Low @@aut@@ Yingjie Zhang @@aut@@ Pakorn Kanchanawong @@aut@@ Vytas A Bankaitis @@aut@@ Hongyan Wang @@aut@@
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callnumber-first	Q - Science
author	Chwee Tat Koe
spellingShingle	Chwee Tat Koe misc QH301-705.5 misc neuroblast misc Drosophila misc asymmetric division misc phosphatidylinositol lipids misc Medicine misc R misc Science misc Q misc Biology (General) Vibrator and PI4KIIIα govern neuroblast polarity by anchoring non-muscle myosin II
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topic_title	QH301-705.5 Vibrator and PI4KIIIα govern neuroblast polarity by anchoring non-muscle myosin II neuroblast Drosophila asymmetric division phosphatidylinositol lipids
topic	misc QH301-705.5 misc neuroblast misc Drosophila misc asymmetric division misc phosphatidylinositol lipids misc Medicine misc R misc Science misc Q misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc neuroblast misc Drosophila misc asymmetric division misc phosphatidylinositol lipids misc Medicine misc R misc Science misc Q misc Biology (General)
topic_browse	misc QH301-705.5 misc neuroblast misc Drosophila misc asymmetric division misc phosphatidylinositol lipids misc Medicine misc R misc Science misc Q misc Biology (General)
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title	Vibrator and PI4KIIIα govern neuroblast polarity by anchoring non-muscle myosin II
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title_full	Vibrator and PI4KIIIα govern neuroblast polarity by anchoring non-muscle myosin II
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author_browse	Chwee Tat Koe Ye Sing Tan Max Lönnfors Seong Kwon Hur Christine Siok Lan Low Yingjie Zhang Pakorn Kanchanawong Vytas A Bankaitis Hongyan Wang
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title_sort	vibrator and pi4kiiiα govern neuroblast polarity by anchoring non-muscle myosin ii
callnumber	QH301-705.5
title_auth	Vibrator and PI4KIIIα govern neuroblast polarity by anchoring non-muscle myosin II
abstract	A central feature of most stem cells is the ability to self-renew and undergo differentiation via asymmetric division. However, during asymmetric division the role of phosphatidylinositol (PI) lipids and their regulators is not well established. Here, we show that the sole type I PI transfer protein, Vibrator, controls asymmetric division of Drosophilaneural stem cells (NSCs) by physically anchoring myosin II regulatory light chain, Sqh, to the NSC cortex. Depletion of vib or disruption of its lipid binding and transfer activities disrupts NSC polarity. We propose that Vib stimulates PI4KIIIα to promote synthesis of a plasma membrane pool of phosphatidylinositol 4-phosphate [PI(4)P] that, in turn, binds and anchors myosin to the NSC cortex. Remarkably, Sqh also binds to PI(4)P in vitro and both Vib and Sqh mediate plasma membrane localization of PI(4)P in NSCs. Thus, reciprocal regulation between Myosin and PI(4)P likely governs asymmetric division of NSCs.
abstractGer	A central feature of most stem cells is the ability to self-renew and undergo differentiation via asymmetric division. However, during asymmetric division the role of phosphatidylinositol (PI) lipids and their regulators is not well established. Here, we show that the sole type I PI transfer protein, Vibrator, controls asymmetric division of Drosophilaneural stem cells (NSCs) by physically anchoring myosin II regulatory light chain, Sqh, to the NSC cortex. Depletion of vib or disruption of its lipid binding and transfer activities disrupts NSC polarity. We propose that Vib stimulates PI4KIIIα to promote synthesis of a plasma membrane pool of phosphatidylinositol 4-phosphate [PI(4)P] that, in turn, binds and anchors myosin to the NSC cortex. Remarkably, Sqh also binds to PI(4)P in vitro and both Vib and Sqh mediate plasma membrane localization of PI(4)P in NSCs. Thus, reciprocal regulation between Myosin and PI(4)P likely governs asymmetric division of NSCs.
abstract_unstemmed	A central feature of most stem cells is the ability to self-renew and undergo differentiation via asymmetric division. However, during asymmetric division the role of phosphatidylinositol (PI) lipids and their regulators is not well established. Here, we show that the sole type I PI transfer protein, Vibrator, controls asymmetric division of Drosophilaneural stem cells (NSCs) by physically anchoring myosin II regulatory light chain, Sqh, to the NSC cortex. Depletion of vib or disruption of its lipid binding and transfer activities disrupts NSC polarity. We propose that Vib stimulates PI4KIIIα to promote synthesis of a plasma membrane pool of phosphatidylinositol 4-phosphate [PI(4)P] that, in turn, binds and anchors myosin to the NSC cortex. Remarkably, Sqh also binds to PI(4)P in vitro and both Vib and Sqh mediate plasma membrane localization of PI(4)P in NSCs. Thus, reciprocal regulation between Myosin and PI(4)P likely governs asymmetric division of NSCs.
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title_short	Vibrator and PI4KIIIα govern neuroblast polarity by anchoring non-muscle myosin II
url	https://doi.org/10.7554/eLife.33555 https://doaj.org/article/f89c1270291546408dfdbcb38f7cbefd https://elifesciences.org/articles/33555 https://doaj.org/toc/2050-084X
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up_date	2024-07-04T00:25:56.878Z
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Vibrator and PI4KIIIα govern neuroblast polarity by anchoring non-muscle myosin II

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