Immune Thymic Profile of the MOG-Induced Experimental Autoimmune Encephalomyelitis Mouse Model

Multiple sclerosis (MS) is a chronic, immune-mediated, demyelinating disease that affects the neurons of the central nervous system. Activated T cells, specific for myelin epitopes, cross the brain barriers, and react against the myelin sheath, leading to demyelination. Since T cells are generated w...
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Autor*in:	Sofia P. das Neves [verfasserIn] Cláudia Serre-Miranda [verfasserIn] Claudia Nobrega [verfasserIn] Susana Roque [verfasserIn] João J. Cerqueira [verfasserIn] Margarida Correia-Neves [verfasserIn] Fernanda Marques [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	autoimmunity multiple sclerosis rodent experimental autoimmune encephalomyelitis thymus

Übergeordnetes Werk:	In: Frontiers in Immunology - Frontiers Media S.A., 2011, 9(2018)
Übergeordnetes Werk:	volume:9 ; year:2018

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fimmu.2018.02335

Katalog-ID:	DOAJ032475926

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520			\|a Multiple sclerosis (MS) is a chronic, immune-mediated, demyelinating disease that affects the neurons of the central nervous system. Activated T cells, specific for myelin epitopes, cross the brain barriers, and react against the myelin sheath, leading to demyelination. Since T cells are generated within the thymus, here we explored, in mice, the alterations occurring in this organ throughout the different phases of the disease. We induced experimental autoimmune encephalomyelitis (EAE) in C57BL/6 females and sacrifice them at the onset (day 16) and chronic phases of disease (day 23), along with non-induced controls. We observed thymic atrophy in EAE mice at the onset that remained until the chronic phase of disease. This atrophy was associated with a preferential loss of the CD4+CD8+ double positive thymocytes, an intermediate population between the more immature CD4−CD8− double negative and the most mature single positive thymocytes. This was accompanied by an increase in the thymic medullary/cortical ratio and by an altered expression levels of genes important for T cell survival. During the chronic phase, the thymi remained atrophic, but reacquired the normal proportion of the main four thymocyte populations and the normal medullary/cortical ratio. Importantly, at the onset phase, and accompanying these thymic alterations, EAE animals presented an increased percentage of demyelinating lesion area in the cerebellum, and an increased expression of interferon gamma (Ifng), interleukin (Il) 12a, and Il17a. This study suggests dynamic thymic alterations occurring in response to EAE, from the induction to the chronic phase, that might help to elucidate the MS pathophysiology.
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Indexfelder

author_variant	s p d n spdn s p d n spdn c s m csm c s m csm c n cn c n cn s r sr s r sr j j c jjc j j c jjc j j c jjc m c n mcn m c n mcn f m fm f m fm
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callnumber-subject-code	RC
publishDate	2018
allfields	10.3389/fimmu.2018.02335 doi (DE-627)DOAJ032475926 (DE-599)DOAJe1ea60cca2784b8d913b8aac2d999336 DE-627 ger DE-627 rakwb eng RC581-607 Sofia P. das Neves verfasserin aut Immune Thymic Profile of the MOG-Induced Experimental Autoimmune Encephalomyelitis Mouse Model 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Multiple sclerosis (MS) is a chronic, immune-mediated, demyelinating disease that affects the neurons of the central nervous system. Activated T cells, specific for myelin epitopes, cross the brain barriers, and react against the myelin sheath, leading to demyelination. Since T cells are generated within the thymus, here we explored, in mice, the alterations occurring in this organ throughout the different phases of the disease. We induced experimental autoimmune encephalomyelitis (EAE) in C57BL/6 females and sacrifice them at the onset (day 16) and chronic phases of disease (day 23), along with non-induced controls. We observed thymic atrophy in EAE mice at the onset that remained until the chronic phase of disease. This atrophy was associated with a preferential loss of the CD4+CD8+ double positive thymocytes, an intermediate population between the more immature CD4−CD8− double negative and the most mature single positive thymocytes. This was accompanied by an increase in the thymic medullary/cortical ratio and by an altered expression levels of genes important for T cell survival. During the chronic phase, the thymi remained atrophic, but reacquired the normal proportion of the main four thymocyte populations and the normal medullary/cortical ratio. Importantly, at the onset phase, and accompanying these thymic alterations, EAE animals presented an increased percentage of demyelinating lesion area in the cerebellum, and an increased expression of interferon gamma (Ifng), interleukin (Il) 12a, and Il17a. This study suggests dynamic thymic alterations occurring in response to EAE, from the induction to the chronic phase, that might help to elucidate the MS pathophysiology. autoimmunity multiple sclerosis rodent experimental autoimmune encephalomyelitis thymus Immunologic diseases. Allergy Sofia P. das Neves verfasserin aut Cláudia Serre-Miranda verfasserin aut Cláudia Serre-Miranda verfasserin aut Claudia Nobrega verfasserin aut Claudia Nobrega verfasserin aut Susana Roque verfasserin aut Susana Roque verfasserin aut João J. Cerqueira verfasserin aut João J. Cerqueira verfasserin aut João J. Cerqueira verfasserin aut Margarida Correia-Neves verfasserin aut Margarida Correia-Neves verfasserin aut Fernanda Marques verfasserin aut Fernanda Marques verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 9(2018) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:9 year:2018 https://doi.org/10.3389/fimmu.2018.02335 kostenfrei https://doaj.org/article/e1ea60cca2784b8d913b8aac2d999336 kostenfrei https://www.frontiersin.org/article/10.3389/fimmu.2018.02335/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2018
spelling	10.3389/fimmu.2018.02335 doi (DE-627)DOAJ032475926 (DE-599)DOAJe1ea60cca2784b8d913b8aac2d999336 DE-627 ger DE-627 rakwb eng RC581-607 Sofia P. das Neves verfasserin aut Immune Thymic Profile of the MOG-Induced Experimental Autoimmune Encephalomyelitis Mouse Model 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Multiple sclerosis (MS) is a chronic, immune-mediated, demyelinating disease that affects the neurons of the central nervous system. Activated T cells, specific for myelin epitopes, cross the brain barriers, and react against the myelin sheath, leading to demyelination. Since T cells are generated within the thymus, here we explored, in mice, the alterations occurring in this organ throughout the different phases of the disease. We induced experimental autoimmune encephalomyelitis (EAE) in C57BL/6 females and sacrifice them at the onset (day 16) and chronic phases of disease (day 23), along with non-induced controls. We observed thymic atrophy in EAE mice at the onset that remained until the chronic phase of disease. This atrophy was associated with a preferential loss of the CD4+CD8+ double positive thymocytes, an intermediate population between the more immature CD4−CD8− double negative and the most mature single positive thymocytes. This was accompanied by an increase in the thymic medullary/cortical ratio and by an altered expression levels of genes important for T cell survival. During the chronic phase, the thymi remained atrophic, but reacquired the normal proportion of the main four thymocyte populations and the normal medullary/cortical ratio. Importantly, at the onset phase, and accompanying these thymic alterations, EAE animals presented an increased percentage of demyelinating lesion area in the cerebellum, and an increased expression of interferon gamma (Ifng), interleukin (Il) 12a, and Il17a. This study suggests dynamic thymic alterations occurring in response to EAE, from the induction to the chronic phase, that might help to elucidate the MS pathophysiology. autoimmunity multiple sclerosis rodent experimental autoimmune encephalomyelitis thymus Immunologic diseases. Allergy Sofia P. das Neves verfasserin aut Cláudia Serre-Miranda verfasserin aut Cláudia Serre-Miranda verfasserin aut Claudia Nobrega verfasserin aut Claudia Nobrega verfasserin aut Susana Roque verfasserin aut Susana Roque verfasserin aut João J. Cerqueira verfasserin aut João J. Cerqueira verfasserin aut João J. Cerqueira verfasserin aut Margarida Correia-Neves verfasserin aut Margarida Correia-Neves verfasserin aut Fernanda Marques verfasserin aut Fernanda Marques verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 9(2018) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:9 year:2018 https://doi.org/10.3389/fimmu.2018.02335 kostenfrei https://doaj.org/article/e1ea60cca2784b8d913b8aac2d999336 kostenfrei https://www.frontiersin.org/article/10.3389/fimmu.2018.02335/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2018
allfields_unstemmed	10.3389/fimmu.2018.02335 doi (DE-627)DOAJ032475926 (DE-599)DOAJe1ea60cca2784b8d913b8aac2d999336 DE-627 ger DE-627 rakwb eng RC581-607 Sofia P. das Neves verfasserin aut Immune Thymic Profile of the MOG-Induced Experimental Autoimmune Encephalomyelitis Mouse Model 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Multiple sclerosis (MS) is a chronic, immune-mediated, demyelinating disease that affects the neurons of the central nervous system. Activated T cells, specific for myelin epitopes, cross the brain barriers, and react against the myelin sheath, leading to demyelination. Since T cells are generated within the thymus, here we explored, in mice, the alterations occurring in this organ throughout the different phases of the disease. We induced experimental autoimmune encephalomyelitis (EAE) in C57BL/6 females and sacrifice them at the onset (day 16) and chronic phases of disease (day 23), along with non-induced controls. We observed thymic atrophy in EAE mice at the onset that remained until the chronic phase of disease. This atrophy was associated with a preferential loss of the CD4+CD8+ double positive thymocytes, an intermediate population between the more immature CD4−CD8− double negative and the most mature single positive thymocytes. This was accompanied by an increase in the thymic medullary/cortical ratio and by an altered expression levels of genes important for T cell survival. During the chronic phase, the thymi remained atrophic, but reacquired the normal proportion of the main four thymocyte populations and the normal medullary/cortical ratio. Importantly, at the onset phase, and accompanying these thymic alterations, EAE animals presented an increased percentage of demyelinating lesion area in the cerebellum, and an increased expression of interferon gamma (Ifng), interleukin (Il) 12a, and Il17a. This study suggests dynamic thymic alterations occurring in response to EAE, from the induction to the chronic phase, that might help to elucidate the MS pathophysiology. autoimmunity multiple sclerosis rodent experimental autoimmune encephalomyelitis thymus Immunologic diseases. Allergy Sofia P. das Neves verfasserin aut Cláudia Serre-Miranda verfasserin aut Cláudia Serre-Miranda verfasserin aut Claudia Nobrega verfasserin aut Claudia Nobrega verfasserin aut Susana Roque verfasserin aut Susana Roque verfasserin aut João J. Cerqueira verfasserin aut João J. Cerqueira verfasserin aut João J. Cerqueira verfasserin aut Margarida Correia-Neves verfasserin aut Margarida Correia-Neves verfasserin aut Fernanda Marques verfasserin aut Fernanda Marques verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 9(2018) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:9 year:2018 https://doi.org/10.3389/fimmu.2018.02335 kostenfrei https://doaj.org/article/e1ea60cca2784b8d913b8aac2d999336 kostenfrei https://www.frontiersin.org/article/10.3389/fimmu.2018.02335/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2018
allfieldsGer	10.3389/fimmu.2018.02335 doi (DE-627)DOAJ032475926 (DE-599)DOAJe1ea60cca2784b8d913b8aac2d999336 DE-627 ger DE-627 rakwb eng RC581-607 Sofia P. das Neves verfasserin aut Immune Thymic Profile of the MOG-Induced Experimental Autoimmune Encephalomyelitis Mouse Model 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Multiple sclerosis (MS) is a chronic, immune-mediated, demyelinating disease that affects the neurons of the central nervous system. Activated T cells, specific for myelin epitopes, cross the brain barriers, and react against the myelin sheath, leading to demyelination. Since T cells are generated within the thymus, here we explored, in mice, the alterations occurring in this organ throughout the different phases of the disease. We induced experimental autoimmune encephalomyelitis (EAE) in C57BL/6 females and sacrifice them at the onset (day 16) and chronic phases of disease (day 23), along with non-induced controls. We observed thymic atrophy in EAE mice at the onset that remained until the chronic phase of disease. This atrophy was associated with a preferential loss of the CD4+CD8+ double positive thymocytes, an intermediate population between the more immature CD4−CD8− double negative and the most mature single positive thymocytes. This was accompanied by an increase in the thymic medullary/cortical ratio and by an altered expression levels of genes important for T cell survival. During the chronic phase, the thymi remained atrophic, but reacquired the normal proportion of the main four thymocyte populations and the normal medullary/cortical ratio. Importantly, at the onset phase, and accompanying these thymic alterations, EAE animals presented an increased percentage of demyelinating lesion area in the cerebellum, and an increased expression of interferon gamma (Ifng), interleukin (Il) 12a, and Il17a. This study suggests dynamic thymic alterations occurring in response to EAE, from the induction to the chronic phase, that might help to elucidate the MS pathophysiology. autoimmunity multiple sclerosis rodent experimental autoimmune encephalomyelitis thymus Immunologic diseases. Allergy Sofia P. das Neves verfasserin aut Cláudia Serre-Miranda verfasserin aut Cláudia Serre-Miranda verfasserin aut Claudia Nobrega verfasserin aut Claudia Nobrega verfasserin aut Susana Roque verfasserin aut Susana Roque verfasserin aut João J. Cerqueira verfasserin aut João J. Cerqueira verfasserin aut João J. Cerqueira verfasserin aut Margarida Correia-Neves verfasserin aut Margarida Correia-Neves verfasserin aut Fernanda Marques verfasserin aut Fernanda Marques verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 9(2018) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:9 year:2018 https://doi.org/10.3389/fimmu.2018.02335 kostenfrei https://doaj.org/article/e1ea60cca2784b8d913b8aac2d999336 kostenfrei https://www.frontiersin.org/article/10.3389/fimmu.2018.02335/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2018
allfieldsSound	10.3389/fimmu.2018.02335 doi (DE-627)DOAJ032475926 (DE-599)DOAJe1ea60cca2784b8d913b8aac2d999336 DE-627 ger DE-627 rakwb eng RC581-607 Sofia P. das Neves verfasserin aut Immune Thymic Profile of the MOG-Induced Experimental Autoimmune Encephalomyelitis Mouse Model 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Multiple sclerosis (MS) is a chronic, immune-mediated, demyelinating disease that affects the neurons of the central nervous system. Activated T cells, specific for myelin epitopes, cross the brain barriers, and react against the myelin sheath, leading to demyelination. Since T cells are generated within the thymus, here we explored, in mice, the alterations occurring in this organ throughout the different phases of the disease. We induced experimental autoimmune encephalomyelitis (EAE) in C57BL/6 females and sacrifice them at the onset (day 16) and chronic phases of disease (day 23), along with non-induced controls. We observed thymic atrophy in EAE mice at the onset that remained until the chronic phase of disease. This atrophy was associated with a preferential loss of the CD4+CD8+ double positive thymocytes, an intermediate population between the more immature CD4−CD8− double negative and the most mature single positive thymocytes. This was accompanied by an increase in the thymic medullary/cortical ratio and by an altered expression levels of genes important for T cell survival. During the chronic phase, the thymi remained atrophic, but reacquired the normal proportion of the main four thymocyte populations and the normal medullary/cortical ratio. Importantly, at the onset phase, and accompanying these thymic alterations, EAE animals presented an increased percentage of demyelinating lesion area in the cerebellum, and an increased expression of interferon gamma (Ifng), interleukin (Il) 12a, and Il17a. This study suggests dynamic thymic alterations occurring in response to EAE, from the induction to the chronic phase, that might help to elucidate the MS pathophysiology. autoimmunity multiple sclerosis rodent experimental autoimmune encephalomyelitis thymus Immunologic diseases. Allergy Sofia P. das Neves verfasserin aut Cláudia Serre-Miranda verfasserin aut Cláudia Serre-Miranda verfasserin aut Claudia Nobrega verfasserin aut Claudia Nobrega verfasserin aut Susana Roque verfasserin aut Susana Roque verfasserin aut João J. Cerqueira verfasserin aut João J. Cerqueira verfasserin aut João J. Cerqueira verfasserin aut Margarida Correia-Neves verfasserin aut Margarida Correia-Neves verfasserin aut Fernanda Marques verfasserin aut Fernanda Marques verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 9(2018) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:9 year:2018 https://doi.org/10.3389/fimmu.2018.02335 kostenfrei https://doaj.org/article/e1ea60cca2784b8d913b8aac2d999336 kostenfrei https://www.frontiersin.org/article/10.3389/fimmu.2018.02335/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2018
language	English
source	In Frontiers in Immunology 9(2018) volume:9 year:2018
sourceStr	In Frontiers in Immunology 9(2018) volume:9 year:2018
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	autoimmunity multiple sclerosis rodent experimental autoimmune encephalomyelitis thymus Immunologic diseases. Allergy
isfreeaccess_bool	true
container_title	Frontiers in Immunology
authorswithroles_txt_mv	Sofia P. das Neves @@aut@@ Cláudia Serre-Miranda @@aut@@ Claudia Nobrega @@aut@@ Susana Roque @@aut@@ João J. Cerqueira @@aut@@ Margarida Correia-Neves @@aut@@ Fernanda Marques @@aut@@
publishDateDaySort_date	2018-01-01T00:00:00Z
hierarchy_top_id	657998354
id	DOAJ032475926
language_de	englisch
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callnumber-first	R - Medicine
author	Sofia P. das Neves
spellingShingle	Sofia P. das Neves misc RC581-607 misc autoimmunity misc multiple sclerosis misc rodent misc experimental autoimmune encephalomyelitis misc thymus misc Immunologic diseases. Allergy Immune Thymic Profile of the MOG-Induced Experimental Autoimmune Encephalomyelitis Mouse Model
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topic_title	RC581-607 Immune Thymic Profile of the MOG-Induced Experimental Autoimmune Encephalomyelitis Mouse Model autoimmunity multiple sclerosis rodent experimental autoimmune encephalomyelitis thymus
topic	misc RC581-607 misc autoimmunity misc multiple sclerosis misc rodent misc experimental autoimmune encephalomyelitis misc thymus misc Immunologic diseases. Allergy
topic_unstemmed	misc RC581-607 misc autoimmunity misc multiple sclerosis misc rodent misc experimental autoimmune encephalomyelitis misc thymus misc Immunologic diseases. Allergy
topic_browse	misc RC581-607 misc autoimmunity misc multiple sclerosis misc rodent misc experimental autoimmune encephalomyelitis misc thymus misc Immunologic diseases. Allergy
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title	Immune Thymic Profile of the MOG-Induced Experimental Autoimmune Encephalomyelitis Mouse Model
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title_full	Immune Thymic Profile of the MOG-Induced Experimental Autoimmune Encephalomyelitis Mouse Model
author_sort	Sofia P. das Neves
journal	Frontiers in Immunology
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author_browse	Sofia P. das Neves Cláudia Serre-Miranda Claudia Nobrega Susana Roque João J. Cerqueira Margarida Correia-Neves Fernanda Marques
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author-letter	Sofia P. das Neves
doi_str_mv	10.3389/fimmu.2018.02335
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title_sort	immune thymic profile of the mog-induced experimental autoimmune encephalomyelitis mouse model
callnumber	RC581-607
title_auth	Immune Thymic Profile of the MOG-Induced Experimental Autoimmune Encephalomyelitis Mouse Model
abstract	Multiple sclerosis (MS) is a chronic, immune-mediated, demyelinating disease that affects the neurons of the central nervous system. Activated T cells, specific for myelin epitopes, cross the brain barriers, and react against the myelin sheath, leading to demyelination. Since T cells are generated within the thymus, here we explored, in mice, the alterations occurring in this organ throughout the different phases of the disease. We induced experimental autoimmune encephalomyelitis (EAE) in C57BL/6 females and sacrifice them at the onset (day 16) and chronic phases of disease (day 23), along with non-induced controls. We observed thymic atrophy in EAE mice at the onset that remained until the chronic phase of disease. This atrophy was associated with a preferential loss of the CD4+CD8+ double positive thymocytes, an intermediate population between the more immature CD4−CD8− double negative and the most mature single positive thymocytes. This was accompanied by an increase in the thymic medullary/cortical ratio and by an altered expression levels of genes important for T cell survival. During the chronic phase, the thymi remained atrophic, but reacquired the normal proportion of the main four thymocyte populations and the normal medullary/cortical ratio. Importantly, at the onset phase, and accompanying these thymic alterations, EAE animals presented an increased percentage of demyelinating lesion area in the cerebellum, and an increased expression of interferon gamma (Ifng), interleukin (Il) 12a, and Il17a. This study suggests dynamic thymic alterations occurring in response to EAE, from the induction to the chronic phase, that might help to elucidate the MS pathophysiology.
abstractGer	Multiple sclerosis (MS) is a chronic, immune-mediated, demyelinating disease that affects the neurons of the central nervous system. Activated T cells, specific for myelin epitopes, cross the brain barriers, and react against the myelin sheath, leading to demyelination. Since T cells are generated within the thymus, here we explored, in mice, the alterations occurring in this organ throughout the different phases of the disease. We induced experimental autoimmune encephalomyelitis (EAE) in C57BL/6 females and sacrifice them at the onset (day 16) and chronic phases of disease (day 23), along with non-induced controls. We observed thymic atrophy in EAE mice at the onset that remained until the chronic phase of disease. This atrophy was associated with a preferential loss of the CD4+CD8+ double positive thymocytes, an intermediate population between the more immature CD4−CD8− double negative and the most mature single positive thymocytes. This was accompanied by an increase in the thymic medullary/cortical ratio and by an altered expression levels of genes important for T cell survival. During the chronic phase, the thymi remained atrophic, but reacquired the normal proportion of the main four thymocyte populations and the normal medullary/cortical ratio. Importantly, at the onset phase, and accompanying these thymic alterations, EAE animals presented an increased percentage of demyelinating lesion area in the cerebellum, and an increased expression of interferon gamma (Ifng), interleukin (Il) 12a, and Il17a. This study suggests dynamic thymic alterations occurring in response to EAE, from the induction to the chronic phase, that might help to elucidate the MS pathophysiology.
abstract_unstemmed	Multiple sclerosis (MS) is a chronic, immune-mediated, demyelinating disease that affects the neurons of the central nervous system. Activated T cells, specific for myelin epitopes, cross the brain barriers, and react against the myelin sheath, leading to demyelination. Since T cells are generated within the thymus, here we explored, in mice, the alterations occurring in this organ throughout the different phases of the disease. We induced experimental autoimmune encephalomyelitis (EAE) in C57BL/6 females and sacrifice them at the onset (day 16) and chronic phases of disease (day 23), along with non-induced controls. We observed thymic atrophy in EAE mice at the onset that remained until the chronic phase of disease. This atrophy was associated with a preferential loss of the CD4+CD8+ double positive thymocytes, an intermediate population between the more immature CD4−CD8− double negative and the most mature single positive thymocytes. This was accompanied by an increase in the thymic medullary/cortical ratio and by an altered expression levels of genes important for T cell survival. During the chronic phase, the thymi remained atrophic, but reacquired the normal proportion of the main four thymocyte populations and the normal medullary/cortical ratio. Importantly, at the onset phase, and accompanying these thymic alterations, EAE animals presented an increased percentage of demyelinating lesion area in the cerebellum, and an increased expression of interferon gamma (Ifng), interleukin (Il) 12a, and Il17a. This study suggests dynamic thymic alterations occurring in response to EAE, from the induction to the chronic phase, that might help to elucidate the MS pathophysiology.
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title_short	Immune Thymic Profile of the MOG-Induced Experimental Autoimmune Encephalomyelitis Mouse Model
url	https://doi.org/10.3389/fimmu.2018.02335 https://doaj.org/article/e1ea60cca2784b8d913b8aac2d999336 https://www.frontiersin.org/article/10.3389/fimmu.2018.02335/full https://doaj.org/toc/1664-3224
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author2	Sofia P. das Neves Cláudia Serre-Miranda Claudia Nobrega Susana Roque João J. Cerqueira Margarida Correia-Neves Fernanda Marques
author2Str	Sofia P. das Neves Cláudia Serre-Miranda Claudia Nobrega Susana Roque João J. Cerqueira Margarida Correia-Neves Fernanda Marques
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up_date	2024-07-04T01:20:35.857Z
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