Antinociceptive Activity of the Skin Secretion of <i<Phyllomedusa rohdei</i< (Amphibia, Anura)

Pain is a distressful experience that can have a major impact on an individual’s quality of life. The need for new and better analgesics has been further intensified in light of the current opioid epidemic. Substances obtained from amphibians have been shown to contain bioactive peptides that exert...
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Autor*in:	Elena Lucia Anna Malpezzi-Marinho [verfasserIn] Cristiane Isabel Silva Zanoni [verfasserIn] Graziella Rigueira Molska [verfasserIn] Camila Paraventi [verfasserIn] Raphael Wuo-Silva [verfasserIn] Laís Fernanda Berro [verfasserIn] Carlos Amilcar Parada [verfasserIn] Eduardo Koji Tamura [verfasserIn] Eduardo Ary Villela Marinho [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	amphibians antinociceptive activity pain skin secretion frog

Übergeordnetes Werk:	In: Toxins - MDPI AG, 2010, 12(2020), 9, p 589
Übergeordnetes Werk:	volume:12 ; year:2020 ; number:9, p 589

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/toxins12090589

Katalog-ID:	DOAJ032611196

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allfields	10.3390/toxins12090589 doi (DE-627)DOAJ032611196 (DE-599)DOAJ847ad88645d44caeb00853b73da91cfe DE-627 ger DE-627 rakwb eng Elena Lucia Anna Malpezzi-Marinho verfasserin aut Antinociceptive Activity of the Skin Secretion of <i<Phyllomedusa rohdei</i< (Amphibia, Anura) 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Pain is a distressful experience that can have a major impact on an individual’s quality of life. The need for new and better analgesics has been further intensified in light of the current opioid epidemic. Substances obtained from amphibians have been shown to contain bioactive peptides that exert analgesic effects. The genus <i<Phyllomedusa</i< represents an important source of peptides and bioactive components. The aim of this study was to investigate the antinociceptive effects of the skin secretion of <i<Phyllomedusa rohdei</i< in rodent models of pain. The crude skin extract of <i<P. rohdei</i< was tested in different pain models: acetic acid-induced writhing test (mice), formalin test (rats), Von Frey electronic test for hypernociception induced by PGE<sub<2</sub< (rats), and hot plate test (mice). Motor-impairing effects were tested using the rota-rod test. The results showed that the skin extract of <i<P. rohdei</i< exerted antinociceptive effects in all pain models tested. Particularly, the highest dose tested of the skin extract decreased acetic acid-induced writhing by 93%, completely blocked formalin-induced nociception both during the acute and inflammatory phases of the test, PGE<sub<2</sub<-induced hypernociception by 73% and increased latency to paw withdrawal in the hot plate test by 300%. The effects observed in the hot plate test were reversed by pretreatment with selective µ and κ, but not δ, opioid receptor antagonists, indicating a mechanism of action dependent on µ and κ opioid receptors. The results were not influenced by sedative effects. Further studies remain necessary to reveal the specific compounds involved in the antinociceptive effects of <i<P. rohdei</i< skin extract as a new therapeutic tool in pain management. amphibians antinociceptive activity pain <i<Phyllomedusa rohdei</i< skin secretion frog Medicine R Cristiane Isabel Silva Zanoni verfasserin aut Graziella Rigueira Molska verfasserin aut Camila Paraventi verfasserin aut Raphael Wuo-Silva verfasserin aut Laís Fernanda Berro verfasserin aut Carlos Amilcar Parada verfasserin aut Eduardo Koji Tamura verfasserin aut Eduardo Ary Villela Marinho verfasserin aut In Toxins MDPI AG, 2010 12(2020), 9, p 589 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:12 year:2020 number:9, p 589 https://doi.org/10.3390/toxins12090589 kostenfrei https://doaj.org/article/847ad88645d44caeb00853b73da91cfe kostenfrei https://www.mdpi.com/2072-6651/12/9/589 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 9, p 589
spelling	10.3390/toxins12090589 doi (DE-627)DOAJ032611196 (DE-599)DOAJ847ad88645d44caeb00853b73da91cfe DE-627 ger DE-627 rakwb eng Elena Lucia Anna Malpezzi-Marinho verfasserin aut Antinociceptive Activity of the Skin Secretion of <i<Phyllomedusa rohdei</i< (Amphibia, Anura) 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Pain is a distressful experience that can have a major impact on an individual’s quality of life. The need for new and better analgesics has been further intensified in light of the current opioid epidemic. Substances obtained from amphibians have been shown to contain bioactive peptides that exert analgesic effects. The genus <i<Phyllomedusa</i< represents an important source of peptides and bioactive components. The aim of this study was to investigate the antinociceptive effects of the skin secretion of <i<Phyllomedusa rohdei</i< in rodent models of pain. The crude skin extract of <i<P. rohdei</i< was tested in different pain models: acetic acid-induced writhing test (mice), formalin test (rats), Von Frey electronic test for hypernociception induced by PGE<sub<2</sub< (rats), and hot plate test (mice). Motor-impairing effects were tested using the rota-rod test. The results showed that the skin extract of <i<P. rohdei</i< exerted antinociceptive effects in all pain models tested. Particularly, the highest dose tested of the skin extract decreased acetic acid-induced writhing by 93%, completely blocked formalin-induced nociception both during the acute and inflammatory phases of the test, PGE<sub<2</sub<-induced hypernociception by 73% and increased latency to paw withdrawal in the hot plate test by 300%. The effects observed in the hot plate test were reversed by pretreatment with selective µ and κ, but not δ, opioid receptor antagonists, indicating a mechanism of action dependent on µ and κ opioid receptors. The results were not influenced by sedative effects. Further studies remain necessary to reveal the specific compounds involved in the antinociceptive effects of <i<P. rohdei</i< skin extract as a new therapeutic tool in pain management. amphibians antinociceptive activity pain <i<Phyllomedusa rohdei</i< skin secretion frog Medicine R Cristiane Isabel Silva Zanoni verfasserin aut Graziella Rigueira Molska verfasserin aut Camila Paraventi verfasserin aut Raphael Wuo-Silva verfasserin aut Laís Fernanda Berro verfasserin aut Carlos Amilcar Parada verfasserin aut Eduardo Koji Tamura verfasserin aut Eduardo Ary Villela Marinho verfasserin aut In Toxins MDPI AG, 2010 12(2020), 9, p 589 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:12 year:2020 number:9, p 589 https://doi.org/10.3390/toxins12090589 kostenfrei https://doaj.org/article/847ad88645d44caeb00853b73da91cfe kostenfrei https://www.mdpi.com/2072-6651/12/9/589 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 9, p 589
allfields_unstemmed	10.3390/toxins12090589 doi (DE-627)DOAJ032611196 (DE-599)DOAJ847ad88645d44caeb00853b73da91cfe DE-627 ger DE-627 rakwb eng Elena Lucia Anna Malpezzi-Marinho verfasserin aut Antinociceptive Activity of the Skin Secretion of <i<Phyllomedusa rohdei</i< (Amphibia, Anura) 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Pain is a distressful experience that can have a major impact on an individual’s quality of life. The need for new and better analgesics has been further intensified in light of the current opioid epidemic. Substances obtained from amphibians have been shown to contain bioactive peptides that exert analgesic effects. The genus <i<Phyllomedusa</i< represents an important source of peptides and bioactive components. The aim of this study was to investigate the antinociceptive effects of the skin secretion of <i<Phyllomedusa rohdei</i< in rodent models of pain. The crude skin extract of <i<P. rohdei</i< was tested in different pain models: acetic acid-induced writhing test (mice), formalin test (rats), Von Frey electronic test for hypernociception induced by PGE<sub<2</sub< (rats), and hot plate test (mice). Motor-impairing effects were tested using the rota-rod test. The results showed that the skin extract of <i<P. rohdei</i< exerted antinociceptive effects in all pain models tested. Particularly, the highest dose tested of the skin extract decreased acetic acid-induced writhing by 93%, completely blocked formalin-induced nociception both during the acute and inflammatory phases of the test, PGE<sub<2</sub<-induced hypernociception by 73% and increased latency to paw withdrawal in the hot plate test by 300%. The effects observed in the hot plate test were reversed by pretreatment with selective µ and κ, but not δ, opioid receptor antagonists, indicating a mechanism of action dependent on µ and κ opioid receptors. The results were not influenced by sedative effects. Further studies remain necessary to reveal the specific compounds involved in the antinociceptive effects of <i<P. rohdei</i< skin extract as a new therapeutic tool in pain management. amphibians antinociceptive activity pain <i<Phyllomedusa rohdei</i< skin secretion frog Medicine R Cristiane Isabel Silva Zanoni verfasserin aut Graziella Rigueira Molska verfasserin aut Camila Paraventi verfasserin aut Raphael Wuo-Silva verfasserin aut Laís Fernanda Berro verfasserin aut Carlos Amilcar Parada verfasserin aut Eduardo Koji Tamura verfasserin aut Eduardo Ary Villela Marinho verfasserin aut In Toxins MDPI AG, 2010 12(2020), 9, p 589 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:12 year:2020 number:9, p 589 https://doi.org/10.3390/toxins12090589 kostenfrei https://doaj.org/article/847ad88645d44caeb00853b73da91cfe kostenfrei https://www.mdpi.com/2072-6651/12/9/589 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 9, p 589
allfieldsGer	10.3390/toxins12090589 doi (DE-627)DOAJ032611196 (DE-599)DOAJ847ad88645d44caeb00853b73da91cfe DE-627 ger DE-627 rakwb eng Elena Lucia Anna Malpezzi-Marinho verfasserin aut Antinociceptive Activity of the Skin Secretion of <i<Phyllomedusa rohdei</i< (Amphibia, Anura) 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Pain is a distressful experience that can have a major impact on an individual’s quality of life. The need for new and better analgesics has been further intensified in light of the current opioid epidemic. Substances obtained from amphibians have been shown to contain bioactive peptides that exert analgesic effects. The genus <i<Phyllomedusa</i< represents an important source of peptides and bioactive components. The aim of this study was to investigate the antinociceptive effects of the skin secretion of <i<Phyllomedusa rohdei</i< in rodent models of pain. The crude skin extract of <i<P. rohdei</i< was tested in different pain models: acetic acid-induced writhing test (mice), formalin test (rats), Von Frey electronic test for hypernociception induced by PGE<sub<2</sub< (rats), and hot plate test (mice). Motor-impairing effects were tested using the rota-rod test. The results showed that the skin extract of <i<P. rohdei</i< exerted antinociceptive effects in all pain models tested. Particularly, the highest dose tested of the skin extract decreased acetic acid-induced writhing by 93%, completely blocked formalin-induced nociception both during the acute and inflammatory phases of the test, PGE<sub<2</sub<-induced hypernociception by 73% and increased latency to paw withdrawal in the hot plate test by 300%. The effects observed in the hot plate test were reversed by pretreatment with selective µ and κ, but not δ, opioid receptor antagonists, indicating a mechanism of action dependent on µ and κ opioid receptors. The results were not influenced by sedative effects. Further studies remain necessary to reveal the specific compounds involved in the antinociceptive effects of <i<P. rohdei</i< skin extract as a new therapeutic tool in pain management. amphibians antinociceptive activity pain <i<Phyllomedusa rohdei</i< skin secretion frog Medicine R Cristiane Isabel Silva Zanoni verfasserin aut Graziella Rigueira Molska verfasserin aut Camila Paraventi verfasserin aut Raphael Wuo-Silva verfasserin aut Laís Fernanda Berro verfasserin aut Carlos Amilcar Parada verfasserin aut Eduardo Koji Tamura verfasserin aut Eduardo Ary Villela Marinho verfasserin aut In Toxins MDPI AG, 2010 12(2020), 9, p 589 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:12 year:2020 number:9, p 589 https://doi.org/10.3390/toxins12090589 kostenfrei https://doaj.org/article/847ad88645d44caeb00853b73da91cfe kostenfrei https://www.mdpi.com/2072-6651/12/9/589 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 9, p 589
allfieldsSound	10.3390/toxins12090589 doi (DE-627)DOAJ032611196 (DE-599)DOAJ847ad88645d44caeb00853b73da91cfe DE-627 ger DE-627 rakwb eng Elena Lucia Anna Malpezzi-Marinho verfasserin aut Antinociceptive Activity of the Skin Secretion of <i<Phyllomedusa rohdei</i< (Amphibia, Anura) 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Pain is a distressful experience that can have a major impact on an individual’s quality of life. The need for new and better analgesics has been further intensified in light of the current opioid epidemic. Substances obtained from amphibians have been shown to contain bioactive peptides that exert analgesic effects. The genus <i<Phyllomedusa</i< represents an important source of peptides and bioactive components. The aim of this study was to investigate the antinociceptive effects of the skin secretion of <i<Phyllomedusa rohdei</i< in rodent models of pain. The crude skin extract of <i<P. rohdei</i< was tested in different pain models: acetic acid-induced writhing test (mice), formalin test (rats), Von Frey electronic test for hypernociception induced by PGE<sub<2</sub< (rats), and hot plate test (mice). Motor-impairing effects were tested using the rota-rod test. The results showed that the skin extract of <i<P. rohdei</i< exerted antinociceptive effects in all pain models tested. Particularly, the highest dose tested of the skin extract decreased acetic acid-induced writhing by 93%, completely blocked formalin-induced nociception both during the acute and inflammatory phases of the test, PGE<sub<2</sub<-induced hypernociception by 73% and increased latency to paw withdrawal in the hot plate test by 300%. The effects observed in the hot plate test were reversed by pretreatment with selective µ and κ, but not δ, opioid receptor antagonists, indicating a mechanism of action dependent on µ and κ opioid receptors. The results were not influenced by sedative effects. Further studies remain necessary to reveal the specific compounds involved in the antinociceptive effects of <i<P. rohdei</i< skin extract as a new therapeutic tool in pain management. amphibians antinociceptive activity pain <i<Phyllomedusa rohdei</i< skin secretion frog Medicine R Cristiane Isabel Silva Zanoni verfasserin aut Graziella Rigueira Molska verfasserin aut Camila Paraventi verfasserin aut Raphael Wuo-Silva verfasserin aut Laís Fernanda Berro verfasserin aut Carlos Amilcar Parada verfasserin aut Eduardo Koji Tamura verfasserin aut Eduardo Ary Villela Marinho verfasserin aut In Toxins MDPI AG, 2010 12(2020), 9, p 589 (DE-627)610604236 (DE-600)2518395-3 20726651 nnns volume:12 year:2020 number:9, p 589 https://doi.org/10.3390/toxins12090589 kostenfrei https://doaj.org/article/847ad88645d44caeb00853b73da91cfe kostenfrei https://www.mdpi.com/2072-6651/12/9/589 kostenfrei https://doaj.org/toc/2072-6651 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 9, p 589
language	English
source	In Toxins 12(2020), 9, p 589 volume:12 year:2020 number:9, p 589
sourceStr	In Toxins 12(2020), 9, p 589 volume:12 year:2020 number:9, p 589
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	amphibians antinociceptive activity pain <i<Phyllomedusa rohdei</i< skin secretion frog Medicine R
isfreeaccess_bool	true
container_title	Toxins
authorswithroles_txt_mv	Elena Lucia Anna Malpezzi-Marinho @@aut@@ Cristiane Isabel Silva Zanoni @@aut@@ Graziella Rigueira Molska @@aut@@ Camila Paraventi @@aut@@ Raphael Wuo-Silva @@aut@@ Laís Fernanda Berro @@aut@@ Carlos Amilcar Parada @@aut@@ Eduardo Koji Tamura @@aut@@ Eduardo Ary Villela Marinho @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	610604236
id	DOAJ032611196
language_de	englisch
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author	Elena Lucia Anna Malpezzi-Marinho
spellingShingle	Elena Lucia Anna Malpezzi-Marinho misc amphibians misc antinociceptive activity misc pain misc <i<Phyllomedusa rohdei</i< misc skin secretion misc frog misc Medicine misc R Antinociceptive Activity of the Skin Secretion of <i<Phyllomedusa rohdei</i< (Amphibia, Anura)
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topic_title	Antinociceptive Activity of the Skin Secretion of <i<Phyllomedusa rohdei</i< (Amphibia, Anura) amphibians antinociceptive activity pain <i<Phyllomedusa rohdei</i< skin secretion frog
topic	misc amphibians misc antinociceptive activity misc pain misc <i<Phyllomedusa rohdei</i< misc skin secretion misc frog misc Medicine misc R
topic_unstemmed	misc amphibians misc antinociceptive activity misc pain misc <i<Phyllomedusa rohdei</i< misc skin secretion misc frog misc Medicine misc R
topic_browse	misc amphibians misc antinociceptive activity misc pain misc <i<Phyllomedusa rohdei</i< misc skin secretion misc frog misc Medicine misc R
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title	Antinociceptive Activity of the Skin Secretion of <i<Phyllomedusa rohdei</i< (Amphibia, Anura)
ctrlnum	(DE-627)DOAJ032611196 (DE-599)DOAJ847ad88645d44caeb00853b73da91cfe
title_full	Antinociceptive Activity of the Skin Secretion of <i<Phyllomedusa rohdei</i< (Amphibia, Anura)
author_sort	Elena Lucia Anna Malpezzi-Marinho
journal	Toxins
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author_browse	Elena Lucia Anna Malpezzi-Marinho Cristiane Isabel Silva Zanoni Graziella Rigueira Molska Camila Paraventi Raphael Wuo-Silva Laís Fernanda Berro Carlos Amilcar Parada Eduardo Koji Tamura Eduardo Ary Villela Marinho
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author-letter	Elena Lucia Anna Malpezzi-Marinho
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title_sort	antinociceptive activity of the skin secretion of <i<phyllomedusa rohdei</i< (amphibia, anura)
title_auth	Antinociceptive Activity of the Skin Secretion of <i<Phyllomedusa rohdei</i< (Amphibia, Anura)
abstract	Pain is a distressful experience that can have a major impact on an individual’s quality of life. The need for new and better analgesics has been further intensified in light of the current opioid epidemic. Substances obtained from amphibians have been shown to contain bioactive peptides that exert analgesic effects. The genus <i<Phyllomedusa</i< represents an important source of peptides and bioactive components. The aim of this study was to investigate the antinociceptive effects of the skin secretion of <i<Phyllomedusa rohdei</i< in rodent models of pain. The crude skin extract of <i<P. rohdei</i< was tested in different pain models: acetic acid-induced writhing test (mice), formalin test (rats), Von Frey electronic test for hypernociception induced by PGE<sub<2</sub< (rats), and hot plate test (mice). Motor-impairing effects were tested using the rota-rod test. The results showed that the skin extract of <i<P. rohdei</i< exerted antinociceptive effects in all pain models tested. Particularly, the highest dose tested of the skin extract decreased acetic acid-induced writhing by 93%, completely blocked formalin-induced nociception both during the acute and inflammatory phases of the test, PGE<sub<2</sub<-induced hypernociception by 73% and increased latency to paw withdrawal in the hot plate test by 300%. The effects observed in the hot plate test were reversed by pretreatment with selective µ and κ, but not δ, opioid receptor antagonists, indicating a mechanism of action dependent on µ and κ opioid receptors. The results were not influenced by sedative effects. Further studies remain necessary to reveal the specific compounds involved in the antinociceptive effects of <i<P. rohdei</i< skin extract as a new therapeutic tool in pain management.
abstractGer	Pain is a distressful experience that can have a major impact on an individual’s quality of life. The need for new and better analgesics has been further intensified in light of the current opioid epidemic. Substances obtained from amphibians have been shown to contain bioactive peptides that exert analgesic effects. The genus <i<Phyllomedusa</i< represents an important source of peptides and bioactive components. The aim of this study was to investigate the antinociceptive effects of the skin secretion of <i<Phyllomedusa rohdei</i< in rodent models of pain. The crude skin extract of <i<P. rohdei</i< was tested in different pain models: acetic acid-induced writhing test (mice), formalin test (rats), Von Frey electronic test for hypernociception induced by PGE<sub<2</sub< (rats), and hot plate test (mice). Motor-impairing effects were tested using the rota-rod test. The results showed that the skin extract of <i<P. rohdei</i< exerted antinociceptive effects in all pain models tested. Particularly, the highest dose tested of the skin extract decreased acetic acid-induced writhing by 93%, completely blocked formalin-induced nociception both during the acute and inflammatory phases of the test, PGE<sub<2</sub<-induced hypernociception by 73% and increased latency to paw withdrawal in the hot plate test by 300%. The effects observed in the hot plate test were reversed by pretreatment with selective µ and κ, but not δ, opioid receptor antagonists, indicating a mechanism of action dependent on µ and κ opioid receptors. The results were not influenced by sedative effects. Further studies remain necessary to reveal the specific compounds involved in the antinociceptive effects of <i<P. rohdei</i< skin extract as a new therapeutic tool in pain management.
abstract_unstemmed	Pain is a distressful experience that can have a major impact on an individual’s quality of life. The need for new and better analgesics has been further intensified in light of the current opioid epidemic. Substances obtained from amphibians have been shown to contain bioactive peptides that exert analgesic effects. The genus <i<Phyllomedusa</i< represents an important source of peptides and bioactive components. The aim of this study was to investigate the antinociceptive effects of the skin secretion of <i<Phyllomedusa rohdei</i< in rodent models of pain. The crude skin extract of <i<P. rohdei</i< was tested in different pain models: acetic acid-induced writhing test (mice), formalin test (rats), Von Frey electronic test for hypernociception induced by PGE<sub<2</sub< (rats), and hot plate test (mice). Motor-impairing effects were tested using the rota-rod test. The results showed that the skin extract of <i<P. rohdei</i< exerted antinociceptive effects in all pain models tested. Particularly, the highest dose tested of the skin extract decreased acetic acid-induced writhing by 93%, completely blocked formalin-induced nociception both during the acute and inflammatory phases of the test, PGE<sub<2</sub<-induced hypernociception by 73% and increased latency to paw withdrawal in the hot plate test by 300%. The effects observed in the hot plate test were reversed by pretreatment with selective µ and κ, but not δ, opioid receptor antagonists, indicating a mechanism of action dependent on µ and κ opioid receptors. The results were not influenced by sedative effects. Further studies remain necessary to reveal the specific compounds involved in the antinociceptive effects of <i<P. rohdei</i< skin extract as a new therapeutic tool in pain management.
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title_short	Antinociceptive Activity of the Skin Secretion of <i<Phyllomedusa rohdei</i< (Amphibia, Anura)
url	https://doi.org/10.3390/toxins12090589 https://doaj.org/article/847ad88645d44caeb00853b73da91cfe https://www.mdpi.com/2072-6651/12/9/589 https://doaj.org/toc/2072-6651
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score	7.401534

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Antinociceptive Activity of the Skin Secretion of <i<Phyllomedusa rohdei</i< (Amphibia, Anura)

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Zugang & Verfügbarkeit

Vorhandene Bände

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