Expression Levels of miR-30c and miR-186 in Adult Patients with Membranous Glomerulonephritis and Focal Segmental Glomerulosclerosis

Seyyedeh Mina Hejazian,1– 3 Mohammadreza Ardalan,2 Mohammadali M Shoja,4 Nasser Samadi,1 Sepideh Zununi Vahed2 1Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; 2Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 3Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran; 4Department of Surgery, University of Illinois at Chicago-Metropolitan Group Hospitals (UIC-MGH), Chicago, IL, USACorrespondence: Sepideh Zununi VahedKidney Research Center, Tabriz University of Medical Sciences, Tabriz, IranEmail sepide.zununigmail.comBackground: Nephrotic syndrome is a common renal problem with different histopathogenesis. MicroRNAs are reported to be involved in the pathophysiology of the syndrome. The aim of this study was to study the levels of miR-30c and miR-186 in NS patients.Methods: Sixty patients with primary NS (membranous glomerulonephritis (MGN, N=30) and focal segmental glomerulosclerosis (FSGS, N=30)) and 24 healthy volunteers were included. Expression levels of the miR-30c and miR-186 were evaluated in plasma and peripheral blood mononuclear cell (PBMC) samples of adult patients with NS using real-time PCR. Moreover, an in-silico analysis was performed to understand the signaling pathways and biological procedures that may be regulated by these miRNAs.Results: In the MGN group, significantly elevated levels of miR-30c and miR-186 were observed in PBMC (P= 0.037) and plasma (P= 0.035) samples, respectively. Moreover, there was a significant increase in miR-30c levels in PBMC samples of the FSGS group when compared to healthy controls (P= 0.004). In ROC curve analysis, combined levels of the studied miRNAs could discriminate cases from controls in plasma and blood cells (AUC≥ 0.72, P< 0.05).Conclusion: A panel of miRNAs may be potential biomarkers in plasma and PBMCs samples of NS patients with different subclasses. More investigations are needed with a large sample size to validate the diagnostic values of the reported miRNAs.Keywords: nephrotic syndrome, proteinuria, membranous glomerulonephritis, focal segmental glomerulosclerosis, microRNAs, biomarker Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Hejazian SM [verfasserIn] Ardalan M [verfasserIn] Shoja MM [verfasserIn] Samadi N [verfasserIn] Zununi Vahed S [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	nephrotic syndrome proteinuria membranous glomerulonephritis focal segmental glomerulosclerosis micrornas biomarker

Übergeordnetes Werk:	In: International Journal of Nephrology and Renovascular Disease - Dove Medical Press, 2009, (2020), Seite 193-201
Übergeordnetes Werk:	year:2020 ; pages:193-201

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Katalog-ID:	DOAJ033035660

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520			\|a Seyyedeh Mina Hejazian,1– 3 Mohammadreza Ardalan,2 Mohammadali M Shoja,4 Nasser Samadi,1 Sepideh Zununi Vahed2 1Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; 2Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 3Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran; 4Department of Surgery, University of Illinois at Chicago-Metropolitan Group Hospitals (UIC-MGH), Chicago, IL, USACorrespondence: Sepideh Zununi VahedKidney Research Center, Tabriz University of Medical Sciences, Tabriz, IranEmail sepide.zununigmail.comBackground: Nephrotic syndrome is a common renal problem with different histopathogenesis. MicroRNAs are reported to be involved in the pathophysiology of the syndrome. The aim of this study was to study the levels of miR-30c and miR-186 in NS patients.Methods: Sixty patients with primary NS (membranous glomerulonephritis (MGN, N=30) and focal segmental glomerulosclerosis (FSGS, N=30)) and 24 healthy volunteers were included. Expression levels of the miR-30c and miR-186 were evaluated in plasma and peripheral blood mononuclear cell (PBMC) samples of adult patients with NS using real-time PCR. Moreover, an in-silico analysis was performed to understand the signaling pathways and biological procedures that may be regulated by these miRNAs.Results: In the MGN group, significantly elevated levels of miR-30c and miR-186 were observed in PBMC (P= 0.037) and plasma (P= 0.035) samples, respectively. Moreover, there was a significant increase in miR-30c levels in PBMC samples of the FSGS group when compared to healthy controls (P= 0.004). In ROC curve analysis, combined levels of the studied miRNAs could discriminate cases from controls in plasma and blood cells (AUC≥ 0.72, P< 0.05).Conclusion: A panel of miRNAs may be potential biomarkers in plasma and PBMCs samples of NS patients with different subclasses. More investigations are needed with a large sample size to validate the diagnostic values of the reported miRNAs.Keywords: nephrotic syndrome, proteinuria, membranous glomerulonephritis, focal segmental glomerulosclerosis, microRNAs, biomarker
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allfields	(DE-627)DOAJ033035660 (DE-599)DOAJb44079a34b0849b19fa4356734cfe9d8 DE-627 ger DE-627 rakwb eng RC870-923 Hejazian SM verfasserin aut Expression Levels of miR-30c and miR-186 in Adult Patients with Membranous Glomerulonephritis and Focal Segmental Glomerulosclerosis 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Seyyedeh Mina Hejazian,1– 3 Mohammadreza Ardalan,2 Mohammadali M Shoja,4 Nasser Samadi,1 Sepideh Zununi Vahed2 1Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; 2Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 3Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran; 4Department of Surgery, University of Illinois at Chicago-Metropolitan Group Hospitals (UIC-MGH), Chicago, IL, USACorrespondence: Sepideh Zununi VahedKidney Research Center, Tabriz University of Medical Sciences, Tabriz, IranEmail sepide.zununigmail.comBackground: Nephrotic syndrome is a common renal problem with different histopathogenesis. MicroRNAs are reported to be involved in the pathophysiology of the syndrome. The aim of this study was to study the levels of miR-30c and miR-186 in NS patients.Methods: Sixty patients with primary NS (membranous glomerulonephritis (MGN, N=30) and focal segmental glomerulosclerosis (FSGS, N=30)) and 24 healthy volunteers were included. Expression levels of the miR-30c and miR-186 were evaluated in plasma and peripheral blood mononuclear cell (PBMC) samples of adult patients with NS using real-time PCR. Moreover, an in-silico analysis was performed to understand the signaling pathways and biological procedures that may be regulated by these miRNAs.Results: In the MGN group, significantly elevated levels of miR-30c and miR-186 were observed in PBMC (P= 0.037) and plasma (P= 0.035) samples, respectively. Moreover, there was a significant increase in miR-30c levels in PBMC samples of the FSGS group when compared to healthy controls (P= 0.004). In ROC curve analysis, combined levels of the studied miRNAs could discriminate cases from controls in plasma and blood cells (AUC≥ 0.72, P< 0.05).Conclusion: A panel of miRNAs may be potential biomarkers in plasma and PBMCs samples of NS patients with different subclasses. More investigations are needed with a large sample size to validate the diagnostic values of the reported miRNAs.Keywords: nephrotic syndrome, proteinuria, membranous glomerulonephritis, focal segmental glomerulosclerosis, microRNAs, biomarker nephrotic syndrome proteinuria membranous glomerulonephritis focal segmental glomerulosclerosis micrornas biomarker Diseases of the genitourinary system. Urology Ardalan M verfasserin aut Shoja MM verfasserin aut Samadi N verfasserin aut Zununi Vahed S verfasserin aut In International Journal of Nephrology and Renovascular Disease Dove Medical Press, 2009 (2020), Seite 193-201 (DE-627)606031928 (DE-600)2508160-3 11787058 nnns year:2020 pages:193-201 https://doaj.org/article/b44079a34b0849b19fa4356734cfe9d8 kostenfrei https://www.dovepress.com/expression-levels-of-mir-30c-and-mir-186-in-adult-patients-with-membra-peer-reviewed-article-IJNRD kostenfrei https://doaj.org/toc/1178-7058 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2020 193-201
spelling	(DE-627)DOAJ033035660 (DE-599)DOAJb44079a34b0849b19fa4356734cfe9d8 DE-627 ger DE-627 rakwb eng RC870-923 Hejazian SM verfasserin aut Expression Levels of miR-30c and miR-186 in Adult Patients with Membranous Glomerulonephritis and Focal Segmental Glomerulosclerosis 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Seyyedeh Mina Hejazian,1– 3 Mohammadreza Ardalan,2 Mohammadali M Shoja,4 Nasser Samadi,1 Sepideh Zununi Vahed2 1Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; 2Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 3Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran; 4Department of Surgery, University of Illinois at Chicago-Metropolitan Group Hospitals (UIC-MGH), Chicago, IL, USACorrespondence: Sepideh Zununi VahedKidney Research Center, Tabriz University of Medical Sciences, Tabriz, IranEmail sepide.zununigmail.comBackground: Nephrotic syndrome is a common renal problem with different histopathogenesis. MicroRNAs are reported to be involved in the pathophysiology of the syndrome. The aim of this study was to study the levels of miR-30c and miR-186 in NS patients.Methods: Sixty patients with primary NS (membranous glomerulonephritis (MGN, N=30) and focal segmental glomerulosclerosis (FSGS, N=30)) and 24 healthy volunteers were included. Expression levels of the miR-30c and miR-186 were evaluated in plasma and peripheral blood mononuclear cell (PBMC) samples of adult patients with NS using real-time PCR. Moreover, an in-silico analysis was performed to understand the signaling pathways and biological procedures that may be regulated by these miRNAs.Results: In the MGN group, significantly elevated levels of miR-30c and miR-186 were observed in PBMC (P= 0.037) and plasma (P= 0.035) samples, respectively. Moreover, there was a significant increase in miR-30c levels in PBMC samples of the FSGS group when compared to healthy controls (P= 0.004). In ROC curve analysis, combined levels of the studied miRNAs could discriminate cases from controls in plasma and blood cells (AUC≥ 0.72, P< 0.05).Conclusion: A panel of miRNAs may be potential biomarkers in plasma and PBMCs samples of NS patients with different subclasses. More investigations are needed with a large sample size to validate the diagnostic values of the reported miRNAs.Keywords: nephrotic syndrome, proteinuria, membranous glomerulonephritis, focal segmental glomerulosclerosis, microRNAs, biomarker nephrotic syndrome proteinuria membranous glomerulonephritis focal segmental glomerulosclerosis micrornas biomarker Diseases of the genitourinary system. Urology Ardalan M verfasserin aut Shoja MM verfasserin aut Samadi N verfasserin aut Zununi Vahed S verfasserin aut In International Journal of Nephrology and Renovascular Disease Dove Medical Press, 2009 (2020), Seite 193-201 (DE-627)606031928 (DE-600)2508160-3 11787058 nnns year:2020 pages:193-201 https://doaj.org/article/b44079a34b0849b19fa4356734cfe9d8 kostenfrei https://www.dovepress.com/expression-levels-of-mir-30c-and-mir-186-in-adult-patients-with-membra-peer-reviewed-article-IJNRD kostenfrei https://doaj.org/toc/1178-7058 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2020 193-201
allfields_unstemmed	(DE-627)DOAJ033035660 (DE-599)DOAJb44079a34b0849b19fa4356734cfe9d8 DE-627 ger DE-627 rakwb eng RC870-923 Hejazian SM verfasserin aut Expression Levels of miR-30c and miR-186 in Adult Patients with Membranous Glomerulonephritis and Focal Segmental Glomerulosclerosis 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Seyyedeh Mina Hejazian,1– 3 Mohammadreza Ardalan,2 Mohammadali M Shoja,4 Nasser Samadi,1 Sepideh Zununi Vahed2 1Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; 2Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 3Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran; 4Department of Surgery, University of Illinois at Chicago-Metropolitan Group Hospitals (UIC-MGH), Chicago, IL, USACorrespondence: Sepideh Zununi VahedKidney Research Center, Tabriz University of Medical Sciences, Tabriz, IranEmail sepide.zununigmail.comBackground: Nephrotic syndrome is a common renal problem with different histopathogenesis. MicroRNAs are reported to be involved in the pathophysiology of the syndrome. The aim of this study was to study the levels of miR-30c and miR-186 in NS patients.Methods: Sixty patients with primary NS (membranous glomerulonephritis (MGN, N=30) and focal segmental glomerulosclerosis (FSGS, N=30)) and 24 healthy volunteers were included. Expression levels of the miR-30c and miR-186 were evaluated in plasma and peripheral blood mononuclear cell (PBMC) samples of adult patients with NS using real-time PCR. Moreover, an in-silico analysis was performed to understand the signaling pathways and biological procedures that may be regulated by these miRNAs.Results: In the MGN group, significantly elevated levels of miR-30c and miR-186 were observed in PBMC (P= 0.037) and plasma (P= 0.035) samples, respectively. Moreover, there was a significant increase in miR-30c levels in PBMC samples of the FSGS group when compared to healthy controls (P= 0.004). In ROC curve analysis, combined levels of the studied miRNAs could discriminate cases from controls in plasma and blood cells (AUC≥ 0.72, P< 0.05).Conclusion: A panel of miRNAs may be potential biomarkers in plasma and PBMCs samples of NS patients with different subclasses. More investigations are needed with a large sample size to validate the diagnostic values of the reported miRNAs.Keywords: nephrotic syndrome, proteinuria, membranous glomerulonephritis, focal segmental glomerulosclerosis, microRNAs, biomarker nephrotic syndrome proteinuria membranous glomerulonephritis focal segmental glomerulosclerosis micrornas biomarker Diseases of the genitourinary system. Urology Ardalan M verfasserin aut Shoja MM verfasserin aut Samadi N verfasserin aut Zununi Vahed S verfasserin aut In International Journal of Nephrology and Renovascular Disease Dove Medical Press, 2009 (2020), Seite 193-201 (DE-627)606031928 (DE-600)2508160-3 11787058 nnns year:2020 pages:193-201 https://doaj.org/article/b44079a34b0849b19fa4356734cfe9d8 kostenfrei https://www.dovepress.com/expression-levels-of-mir-30c-and-mir-186-in-adult-patients-with-membra-peer-reviewed-article-IJNRD kostenfrei https://doaj.org/toc/1178-7058 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2020 193-201
allfieldsGer	(DE-627)DOAJ033035660 (DE-599)DOAJb44079a34b0849b19fa4356734cfe9d8 DE-627 ger DE-627 rakwb eng RC870-923 Hejazian SM verfasserin aut Expression Levels of miR-30c and miR-186 in Adult Patients with Membranous Glomerulonephritis and Focal Segmental Glomerulosclerosis 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Seyyedeh Mina Hejazian,1– 3 Mohammadreza Ardalan,2 Mohammadali M Shoja,4 Nasser Samadi,1 Sepideh Zununi Vahed2 1Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; 2Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 3Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran; 4Department of Surgery, University of Illinois at Chicago-Metropolitan Group Hospitals (UIC-MGH), Chicago, IL, USACorrespondence: Sepideh Zununi VahedKidney Research Center, Tabriz University of Medical Sciences, Tabriz, IranEmail sepide.zununigmail.comBackground: Nephrotic syndrome is a common renal problem with different histopathogenesis. MicroRNAs are reported to be involved in the pathophysiology of the syndrome. The aim of this study was to study the levels of miR-30c and miR-186 in NS patients.Methods: Sixty patients with primary NS (membranous glomerulonephritis (MGN, N=30) and focal segmental glomerulosclerosis (FSGS, N=30)) and 24 healthy volunteers were included. Expression levels of the miR-30c and miR-186 were evaluated in plasma and peripheral blood mononuclear cell (PBMC) samples of adult patients with NS using real-time PCR. Moreover, an in-silico analysis was performed to understand the signaling pathways and biological procedures that may be regulated by these miRNAs.Results: In the MGN group, significantly elevated levels of miR-30c and miR-186 were observed in PBMC (P= 0.037) and plasma (P= 0.035) samples, respectively. Moreover, there was a significant increase in miR-30c levels in PBMC samples of the FSGS group when compared to healthy controls (P= 0.004). In ROC curve analysis, combined levels of the studied miRNAs could discriminate cases from controls in plasma and blood cells (AUC≥ 0.72, P< 0.05).Conclusion: A panel of miRNAs may be potential biomarkers in plasma and PBMCs samples of NS patients with different subclasses. More investigations are needed with a large sample size to validate the diagnostic values of the reported miRNAs.Keywords: nephrotic syndrome, proteinuria, membranous glomerulonephritis, focal segmental glomerulosclerosis, microRNAs, biomarker nephrotic syndrome proteinuria membranous glomerulonephritis focal segmental glomerulosclerosis micrornas biomarker Diseases of the genitourinary system. Urology Ardalan M verfasserin aut Shoja MM verfasserin aut Samadi N verfasserin aut Zununi Vahed S verfasserin aut In International Journal of Nephrology and Renovascular Disease Dove Medical Press, 2009 (2020), Seite 193-201 (DE-627)606031928 (DE-600)2508160-3 11787058 nnns year:2020 pages:193-201 https://doaj.org/article/b44079a34b0849b19fa4356734cfe9d8 kostenfrei https://www.dovepress.com/expression-levels-of-mir-30c-and-mir-186-in-adult-patients-with-membra-peer-reviewed-article-IJNRD kostenfrei https://doaj.org/toc/1178-7058 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2020 193-201
allfieldsSound	(DE-627)DOAJ033035660 (DE-599)DOAJb44079a34b0849b19fa4356734cfe9d8 DE-627 ger DE-627 rakwb eng RC870-923 Hejazian SM verfasserin aut Expression Levels of miR-30c and miR-186 in Adult Patients with Membranous Glomerulonephritis and Focal Segmental Glomerulosclerosis 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Seyyedeh Mina Hejazian,1– 3 Mohammadreza Ardalan,2 Mohammadali M Shoja,4 Nasser Samadi,1 Sepideh Zununi Vahed2 1Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; 2Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 3Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran; 4Department of Surgery, University of Illinois at Chicago-Metropolitan Group Hospitals (UIC-MGH), Chicago, IL, USACorrespondence: Sepideh Zununi VahedKidney Research Center, Tabriz University of Medical Sciences, Tabriz, IranEmail sepide.zununigmail.comBackground: Nephrotic syndrome is a common renal problem with different histopathogenesis. MicroRNAs are reported to be involved in the pathophysiology of the syndrome. The aim of this study was to study the levels of miR-30c and miR-186 in NS patients.Methods: Sixty patients with primary NS (membranous glomerulonephritis (MGN, N=30) and focal segmental glomerulosclerosis (FSGS, N=30)) and 24 healthy volunteers were included. Expression levels of the miR-30c and miR-186 were evaluated in plasma and peripheral blood mononuclear cell (PBMC) samples of adult patients with NS using real-time PCR. Moreover, an in-silico analysis was performed to understand the signaling pathways and biological procedures that may be regulated by these miRNAs.Results: In the MGN group, significantly elevated levels of miR-30c and miR-186 were observed in PBMC (P= 0.037) and plasma (P= 0.035) samples, respectively. Moreover, there was a significant increase in miR-30c levels in PBMC samples of the FSGS group when compared to healthy controls (P= 0.004). In ROC curve analysis, combined levels of the studied miRNAs could discriminate cases from controls in plasma and blood cells (AUC≥ 0.72, P< 0.05).Conclusion: A panel of miRNAs may be potential biomarkers in plasma and PBMCs samples of NS patients with different subclasses. More investigations are needed with a large sample size to validate the diagnostic values of the reported miRNAs.Keywords: nephrotic syndrome, proteinuria, membranous glomerulonephritis, focal segmental glomerulosclerosis, microRNAs, biomarker nephrotic syndrome proteinuria membranous glomerulonephritis focal segmental glomerulosclerosis micrornas biomarker Diseases of the genitourinary system. Urology Ardalan M verfasserin aut Shoja MM verfasserin aut Samadi N verfasserin aut Zununi Vahed S verfasserin aut In International Journal of Nephrology and Renovascular Disease Dove Medical Press, 2009 (2020), Seite 193-201 (DE-627)606031928 (DE-600)2508160-3 11787058 nnns year:2020 pages:193-201 https://doaj.org/article/b44079a34b0849b19fa4356734cfe9d8 kostenfrei https://www.dovepress.com/expression-levels-of-mir-30c-and-mir-186-in-adult-patients-with-membra-peer-reviewed-article-IJNRD kostenfrei https://doaj.org/toc/1178-7058 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2020 193-201
language	English
source	In International Journal of Nephrology and Renovascular Disease (2020), Seite 193-201 year:2020 pages:193-201
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topic_facet	nephrotic syndrome proteinuria membranous glomerulonephritis focal segmental glomerulosclerosis micrornas biomarker Diseases of the genitourinary system. Urology
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container_title	International Journal of Nephrology and Renovascular Disease
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MicroRNAs are reported to be involved in the pathophysiology of the syndrome. The aim of this study was to study the levels of miR-30c and miR-186 in NS patients.Methods: Sixty patients with primary NS (membranous glomerulonephritis (MGN, N=30) and focal segmental glomerulosclerosis (FSGS, N=30)) and 24 healthy volunteers were included. Expression levels of the miR-30c and miR-186 were evaluated in plasma and peripheral blood mononuclear cell (PBMC) samples of adult patients with NS using real-time PCR. Moreover, an in-silico analysis was performed to understand the signaling pathways and biological procedures that may be regulated by these miRNAs.Results: In the MGN group, significantly elevated levels of miR-30c and miR-186 were observed in PBMC (P= 0.037) and plasma (P= 0.035) samples, respectively. Moreover, there was a significant increase in miR-30c levels in PBMC samples of the FSGS group when compared to healthy controls (P= 0.004). 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topic_title	RC870-923 Expression Levels of miR-30c and miR-186 in Adult Patients with Membranous Glomerulonephritis and Focal Segmental Glomerulosclerosis nephrotic syndrome proteinuria membranous glomerulonephritis focal segmental glomerulosclerosis micrornas biomarker
topic	misc RC870-923 misc nephrotic syndrome misc proteinuria misc membranous glomerulonephritis misc focal segmental glomerulosclerosis misc micrornas misc biomarker misc Diseases of the genitourinary system. Urology
topic_unstemmed	misc RC870-923 misc nephrotic syndrome misc proteinuria misc membranous glomerulonephritis misc focal segmental glomerulosclerosis misc micrornas misc biomarker misc Diseases of the genitourinary system. Urology
topic_browse	misc RC870-923 misc nephrotic syndrome misc proteinuria misc membranous glomerulonephritis misc focal segmental glomerulosclerosis misc micrornas misc biomarker misc Diseases of the genitourinary system. Urology
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title	Expression Levels of miR-30c and miR-186 in Adult Patients with Membranous Glomerulonephritis and Focal Segmental Glomerulosclerosis
ctrlnum	(DE-627)DOAJ033035660 (DE-599)DOAJb44079a34b0849b19fa4356734cfe9d8
title_full	Expression Levels of miR-30c and miR-186 in Adult Patients with Membranous Glomerulonephritis and Focal Segmental Glomerulosclerosis
author_sort	Hejazian SM
journal	International Journal of Nephrology and Renovascular Disease
journalStr	International Journal of Nephrology and Renovascular Disease
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author_browse	Hejazian SM Ardalan M Shoja MM Samadi N Zununi Vahed S
class	RC870-923
format_se	Elektronische Aufsätze
author-letter	Hejazian SM
author2-role	verfasserin
title_sort	expression levels of mir-30c and mir-186 in adult patients with membranous glomerulonephritis and focal segmental glomerulosclerosis
callnumber	RC870-923
title_auth	Expression Levels of miR-30c and miR-186 in Adult Patients with Membranous Glomerulonephritis and Focal Segmental Glomerulosclerosis
abstract	Seyyedeh Mina Hejazian,1– 3 Mohammadreza Ardalan,2 Mohammadali M Shoja,4 Nasser Samadi,1 Sepideh Zununi Vahed2 1Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; 2Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 3Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran; 4Department of Surgery, University of Illinois at Chicago-Metropolitan Group Hospitals (UIC-MGH), Chicago, IL, USACorrespondence: Sepideh Zununi VahedKidney Research Center, Tabriz University of Medical Sciences, Tabriz, IranEmail sepide.zununigmail.comBackground: Nephrotic syndrome is a common renal problem with different histopathogenesis. MicroRNAs are reported to be involved in the pathophysiology of the syndrome. The aim of this study was to study the levels of miR-30c and miR-186 in NS patients.Methods: Sixty patients with primary NS (membranous glomerulonephritis (MGN, N=30) and focal segmental glomerulosclerosis (FSGS, N=30)) and 24 healthy volunteers were included. Expression levels of the miR-30c and miR-186 were evaluated in plasma and peripheral blood mononuclear cell (PBMC) samples of adult patients with NS using real-time PCR. Moreover, an in-silico analysis was performed to understand the signaling pathways and biological procedures that may be regulated by these miRNAs.Results: In the MGN group, significantly elevated levels of miR-30c and miR-186 were observed in PBMC (P= 0.037) and plasma (P= 0.035) samples, respectively. Moreover, there was a significant increase in miR-30c levels in PBMC samples of the FSGS group when compared to healthy controls (P= 0.004). In ROC curve analysis, combined levels of the studied miRNAs could discriminate cases from controls in plasma and blood cells (AUC≥ 0.72, P< 0.05).Conclusion: A panel of miRNAs may be potential biomarkers in plasma and PBMCs samples of NS patients with different subclasses. More investigations are needed with a large sample size to validate the diagnostic values of the reported miRNAs.Keywords: nephrotic syndrome, proteinuria, membranous glomerulonephritis, focal segmental glomerulosclerosis, microRNAs, biomarker
abstractGer	Seyyedeh Mina Hejazian,1– 3 Mohammadreza Ardalan,2 Mohammadali M Shoja,4 Nasser Samadi,1 Sepideh Zununi Vahed2 1Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; 2Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 3Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran; 4Department of Surgery, University of Illinois at Chicago-Metropolitan Group Hospitals (UIC-MGH), Chicago, IL, USACorrespondence: Sepideh Zununi VahedKidney Research Center, Tabriz University of Medical Sciences, Tabriz, IranEmail sepide.zununigmail.comBackground: Nephrotic syndrome is a common renal problem with different histopathogenesis. MicroRNAs are reported to be involved in the pathophysiology of the syndrome. The aim of this study was to study the levels of miR-30c and miR-186 in NS patients.Methods: Sixty patients with primary NS (membranous glomerulonephritis (MGN, N=30) and focal segmental glomerulosclerosis (FSGS, N=30)) and 24 healthy volunteers were included. Expression levels of the miR-30c and miR-186 were evaluated in plasma and peripheral blood mononuclear cell (PBMC) samples of adult patients with NS using real-time PCR. Moreover, an in-silico analysis was performed to understand the signaling pathways and biological procedures that may be regulated by these miRNAs.Results: In the MGN group, significantly elevated levels of miR-30c and miR-186 were observed in PBMC (P= 0.037) and plasma (P= 0.035) samples, respectively. Moreover, there was a significant increase in miR-30c levels in PBMC samples of the FSGS group when compared to healthy controls (P= 0.004). In ROC curve analysis, combined levels of the studied miRNAs could discriminate cases from controls in plasma and blood cells (AUC≥ 0.72, P< 0.05).Conclusion: A panel of miRNAs may be potential biomarkers in plasma and PBMCs samples of NS patients with different subclasses. More investigations are needed with a large sample size to validate the diagnostic values of the reported miRNAs.Keywords: nephrotic syndrome, proteinuria, membranous glomerulonephritis, focal segmental glomerulosclerosis, microRNAs, biomarker
abstract_unstemmed	Seyyedeh Mina Hejazian,1– 3 Mohammadreza Ardalan,2 Mohammadali M Shoja,4 Nasser Samadi,1 Sepideh Zununi Vahed2 1Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; 2Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 3Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran; 4Department of Surgery, University of Illinois at Chicago-Metropolitan Group Hospitals (UIC-MGH), Chicago, IL, USACorrespondence: Sepideh Zununi VahedKidney Research Center, Tabriz University of Medical Sciences, Tabriz, IranEmail sepide.zununigmail.comBackground: Nephrotic syndrome is a common renal problem with different histopathogenesis. MicroRNAs are reported to be involved in the pathophysiology of the syndrome. The aim of this study was to study the levels of miR-30c and miR-186 in NS patients.Methods: Sixty patients with primary NS (membranous glomerulonephritis (MGN, N=30) and focal segmental glomerulosclerosis (FSGS, N=30)) and 24 healthy volunteers were included. Expression levels of the miR-30c and miR-186 were evaluated in plasma and peripheral blood mononuclear cell (PBMC) samples of adult patients with NS using real-time PCR. Moreover, an in-silico analysis was performed to understand the signaling pathways and biological procedures that may be regulated by these miRNAs.Results: In the MGN group, significantly elevated levels of miR-30c and miR-186 were observed in PBMC (P= 0.037) and plasma (P= 0.035) samples, respectively. Moreover, there was a significant increase in miR-30c levels in PBMC samples of the FSGS group when compared to healthy controls (P= 0.004). In ROC curve analysis, combined levels of the studied miRNAs could discriminate cases from controls in plasma and blood cells (AUC≥ 0.72, P< 0.05).Conclusion: A panel of miRNAs may be potential biomarkers in plasma and PBMCs samples of NS patients with different subclasses. More investigations are needed with a large sample size to validate the diagnostic values of the reported miRNAs.Keywords: nephrotic syndrome, proteinuria, membranous glomerulonephritis, focal segmental glomerulosclerosis, microRNAs, biomarker
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title_short	Expression Levels of miR-30c and miR-186 in Adult Patients with Membranous Glomerulonephritis and Focal Segmental Glomerulosclerosis
url	https://doaj.org/article/b44079a34b0849b19fa4356734cfe9d8 https://www.dovepress.com/expression-levels-of-mir-30c-and-mir-186-in-adult-patients-with-membra-peer-reviewed-article-IJNRD https://doaj.org/toc/1178-7058
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up_date	2024-07-03T15:28:30.826Z
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