Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules

AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP...
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Gespeichert in:

Autor*in:	Zeng-Yang Yu [verfasserIn] Bin Lu [verfasserIn] Chen-Yuan Gong [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9

Übergeordnetes Werk:	In: Guoji Yanke Zazhi - Press of International Journal of Ophthalmology (IJO PRESS), 2017, 16(2016), 1, Seite 6
Übergeordnetes Werk:	volume:16 ; year:2016 ; number:1 ; pages:6

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3980/j.issn.1672-5123.2016.1.01

Katalog-ID:	DOAJ03353909X

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allfields	10.3980/j.issn.1672-5123.2016.1.01 doi (DE-627)DOAJ03353909X (DE-599)DOAJe467fda682e4428fa6f3d55ed5bbb7e7 DE-627 ger DE-627 rakwb eng RE1-994 Zeng-Yang Yu verfasserin aut Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP9)during the development of PDR.<p<METHODS:C57BL/6J mice were intraperitoneal injected with STZ(55 mg/kg)for 5 consecutive days, and blood glucose concentrations were measured after 7d of the injection. The diabetic mice were further housed for 3, 4, 5mo respectively after the development of diabetes. Histological evaluation of retinas was performed. The retinal vessels were detected by immunofluorescence staining with the cluster of differentiation 31(CD31). The mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 in mice retinas was detected by Real-time PCR analysis.<p<RESULTS: Retinal histological observation and CD31 staining both demonstrate that there are more vessels in diabetic mice than in normal control mice at 5mo after the development of diabetes. As compared with normal control, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased in diabetic mice at 5mo after the development of diabetes.<p<CONCLUSION: This study demonstrates that PDR is occurred at 5mo after the development of diabetes in STZ-induced diabetic mice. In addition, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased after the development of PDR. diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9 Ophthalmology Bin Lu verfasserin aut Chen-Yuan Gong verfasserin aut In Guoji Yanke Zazhi Press of International Journal of Ophthalmology (IJO PRESS), 2017 16(2016), 1, Seite 6 (DE-627)1680946854 16725123 nnns volume:16 year:2016 number:1 pages:6 https://doi.org/10.3980/j.issn.1672-5123.2016.1.01 kostenfrei https://doaj.org/article/e467fda682e4428fa6f3d55ed5bbb7e7 kostenfrei http://ies.ijo.cn/cn_publish/2016/1/201601001.pdf kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_2817 AR 16 2016 1 6
spelling	10.3980/j.issn.1672-5123.2016.1.01 doi (DE-627)DOAJ03353909X (DE-599)DOAJe467fda682e4428fa6f3d55ed5bbb7e7 DE-627 ger DE-627 rakwb eng RE1-994 Zeng-Yang Yu verfasserin aut Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP9)during the development of PDR.<p<METHODS:C57BL/6J mice were intraperitoneal injected with STZ(55 mg/kg)for 5 consecutive days, and blood glucose concentrations were measured after 7d of the injection. The diabetic mice were further housed for 3, 4, 5mo respectively after the development of diabetes. Histological evaluation of retinas was performed. The retinal vessels were detected by immunofluorescence staining with the cluster of differentiation 31(CD31). The mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 in mice retinas was detected by Real-time PCR analysis.<p<RESULTS: Retinal histological observation and CD31 staining both demonstrate that there are more vessels in diabetic mice than in normal control mice at 5mo after the development of diabetes. As compared with normal control, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased in diabetic mice at 5mo after the development of diabetes.<p<CONCLUSION: This study demonstrates that PDR is occurred at 5mo after the development of diabetes in STZ-induced diabetic mice. In addition, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased after the development of PDR. diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9 Ophthalmology Bin Lu verfasserin aut Chen-Yuan Gong verfasserin aut In Guoji Yanke Zazhi Press of International Journal of Ophthalmology (IJO PRESS), 2017 16(2016), 1, Seite 6 (DE-627)1680946854 16725123 nnns volume:16 year:2016 number:1 pages:6 https://doi.org/10.3980/j.issn.1672-5123.2016.1.01 kostenfrei https://doaj.org/article/e467fda682e4428fa6f3d55ed5bbb7e7 kostenfrei http://ies.ijo.cn/cn_publish/2016/1/201601001.pdf kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_2817 AR 16 2016 1 6
allfields_unstemmed	10.3980/j.issn.1672-5123.2016.1.01 doi (DE-627)DOAJ03353909X (DE-599)DOAJe467fda682e4428fa6f3d55ed5bbb7e7 DE-627 ger DE-627 rakwb eng RE1-994 Zeng-Yang Yu verfasserin aut Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP9)during the development of PDR.<p<METHODS:C57BL/6J mice were intraperitoneal injected with STZ(55 mg/kg)for 5 consecutive days, and blood glucose concentrations were measured after 7d of the injection. The diabetic mice were further housed for 3, 4, 5mo respectively after the development of diabetes. Histological evaluation of retinas was performed. The retinal vessels were detected by immunofluorescence staining with the cluster of differentiation 31(CD31). The mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 in mice retinas was detected by Real-time PCR analysis.<p<RESULTS: Retinal histological observation and CD31 staining both demonstrate that there are more vessels in diabetic mice than in normal control mice at 5mo after the development of diabetes. As compared with normal control, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased in diabetic mice at 5mo after the development of diabetes.<p<CONCLUSION: This study demonstrates that PDR is occurred at 5mo after the development of diabetes in STZ-induced diabetic mice. In addition, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased after the development of PDR. diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9 Ophthalmology Bin Lu verfasserin aut Chen-Yuan Gong verfasserin aut In Guoji Yanke Zazhi Press of International Journal of Ophthalmology (IJO PRESS), 2017 16(2016), 1, Seite 6 (DE-627)1680946854 16725123 nnns volume:16 year:2016 number:1 pages:6 https://doi.org/10.3980/j.issn.1672-5123.2016.1.01 kostenfrei https://doaj.org/article/e467fda682e4428fa6f3d55ed5bbb7e7 kostenfrei http://ies.ijo.cn/cn_publish/2016/1/201601001.pdf kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_2817 AR 16 2016 1 6
allfieldsGer	10.3980/j.issn.1672-5123.2016.1.01 doi (DE-627)DOAJ03353909X (DE-599)DOAJe467fda682e4428fa6f3d55ed5bbb7e7 DE-627 ger DE-627 rakwb eng RE1-994 Zeng-Yang Yu verfasserin aut Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP9)during the development of PDR.<p<METHODS:C57BL/6J mice were intraperitoneal injected with STZ(55 mg/kg)for 5 consecutive days, and blood glucose concentrations were measured after 7d of the injection. The diabetic mice were further housed for 3, 4, 5mo respectively after the development of diabetes. Histological evaluation of retinas was performed. The retinal vessels were detected by immunofluorescence staining with the cluster of differentiation 31(CD31). The mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 in mice retinas was detected by Real-time PCR analysis.<p<RESULTS: Retinal histological observation and CD31 staining both demonstrate that there are more vessels in diabetic mice than in normal control mice at 5mo after the development of diabetes. As compared with normal control, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased in diabetic mice at 5mo after the development of diabetes.<p<CONCLUSION: This study demonstrates that PDR is occurred at 5mo after the development of diabetes in STZ-induced diabetic mice. In addition, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased after the development of PDR. diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9 Ophthalmology Bin Lu verfasserin aut Chen-Yuan Gong verfasserin aut In Guoji Yanke Zazhi Press of International Journal of Ophthalmology (IJO PRESS), 2017 16(2016), 1, Seite 6 (DE-627)1680946854 16725123 nnns volume:16 year:2016 number:1 pages:6 https://doi.org/10.3980/j.issn.1672-5123.2016.1.01 kostenfrei https://doaj.org/article/e467fda682e4428fa6f3d55ed5bbb7e7 kostenfrei http://ies.ijo.cn/cn_publish/2016/1/201601001.pdf kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_2817 AR 16 2016 1 6
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title	Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules
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title_full	Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules
author_sort	Zeng-Yang Yu
journal	Guoji Yanke Zazhi
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author_browse	Zeng-Yang Yu Bin Lu Chen-Yuan Gong
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doi_str_mv	10.3980/j.issn.1672-5123.2016.1.01
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title_sort	streptozotocin induced diabetic retinopathy in c57 mice and the expression of some pro-angiogenic molecules
callnumber	RE1-994
title_auth	Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules
abstract	AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP9)during the development of PDR.<p<METHODS:C57BL/6J mice were intraperitoneal injected with STZ(55 mg/kg)for 5 consecutive days, and blood glucose concentrations were measured after 7d of the injection. The diabetic mice were further housed for 3, 4, 5mo respectively after the development of diabetes. Histological evaluation of retinas was performed. The retinal vessels were detected by immunofluorescence staining with the cluster of differentiation 31(CD31). The mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 in mice retinas was detected by Real-time PCR analysis.<p<RESULTS: Retinal histological observation and CD31 staining both demonstrate that there are more vessels in diabetic mice than in normal control mice at 5mo after the development of diabetes. As compared with normal control, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased in diabetic mice at 5mo after the development of diabetes.<p<CONCLUSION: This study demonstrates that PDR is occurred at 5mo after the development of diabetes in STZ-induced diabetic mice. In addition, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased after the development of PDR.
abstractGer	AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP9)during the development of PDR.<p<METHODS:C57BL/6J mice were intraperitoneal injected with STZ(55 mg/kg)for 5 consecutive days, and blood glucose concentrations were measured after 7d of the injection. The diabetic mice were further housed for 3, 4, 5mo respectively after the development of diabetes. Histological evaluation of retinas was performed. The retinal vessels were detected by immunofluorescence staining with the cluster of differentiation 31(CD31). The mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 in mice retinas was detected by Real-time PCR analysis.<p<RESULTS: Retinal histological observation and CD31 staining both demonstrate that there are more vessels in diabetic mice than in normal control mice at 5mo after the development of diabetes. As compared with normal control, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased in diabetic mice at 5mo after the development of diabetes.<p<CONCLUSION: This study demonstrates that PDR is occurred at 5mo after the development of diabetes in STZ-induced diabetic mice. In addition, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased after the development of PDR.
abstract_unstemmed	AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP9)during the development of PDR.<p<METHODS:C57BL/6J mice were intraperitoneal injected with STZ(55 mg/kg)for 5 consecutive days, and blood glucose concentrations were measured after 7d of the injection. The diabetic mice were further housed for 3, 4, 5mo respectively after the development of diabetes. Histological evaluation of retinas was performed. The retinal vessels were detected by immunofluorescence staining with the cluster of differentiation 31(CD31). The mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 in mice retinas was detected by Real-time PCR analysis.<p<RESULTS: Retinal histological observation and CD31 staining both demonstrate that there are more vessels in diabetic mice than in normal control mice at 5mo after the development of diabetes. As compared with normal control, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased in diabetic mice at 5mo after the development of diabetes.<p<CONCLUSION: This study demonstrates that PDR is occurred at 5mo after the development of diabetes in STZ-induced diabetic mice. In addition, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased after the development of PDR.
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title_short	Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules
url	https://doi.org/10.3980/j.issn.1672-5123.2016.1.01 https://doaj.org/article/e467fda682e4428fa6f3d55ed5bbb7e7 http://ies.ijo.cn/cn_publish/2016/1/201601001.pdf https://doaj.org/toc/1672-5123
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up_date	2024-07-03T18:20:22.465Z
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