Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules
AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP...
Ausführliche Beschreibung
Autor*in: |
Zeng-Yang Yu [verfasserIn] Bin Lu [verfasserIn] Chen-Yuan Gong [verfasserIn] |
---|
Format: |
E-Artikel |
---|---|
Sprache: |
Englisch |
Erschienen: |
2016 |
---|
Schlagwörter: |
---|
Übergeordnetes Werk: |
In: Guoji Yanke Zazhi - Press of International Journal of Ophthalmology (IJO PRESS), 2017, 16(2016), 1, Seite 6 |
---|---|
Übergeordnetes Werk: |
volume:16 ; year:2016 ; number:1 ; pages:6 |
Links: |
Link aufrufen |
---|
DOI / URN: |
10.3980/j.issn.1672-5123.2016.1.01 |
---|
Katalog-ID: |
DOAJ03353909X |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | DOAJ03353909X | ||
003 | DE-627 | ||
005 | 20230307180920.0 | ||
007 | cr uuu---uuuuu | ||
008 | 230226s2016 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.3980/j.issn.1672-5123.2016.1.01 |2 doi | |
035 | |a (DE-627)DOAJ03353909X | ||
035 | |a (DE-599)DOAJe467fda682e4428fa6f3d55ed5bbb7e7 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
050 | 0 | |a RE1-994 | |
100 | 0 | |a Zeng-Yang Yu |e verfasserin |4 aut | |
245 | 1 | 0 | |a Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules |
264 | 1 | |c 2016 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a Computermedien |b c |2 rdamedia | ||
338 | |a Online-Ressource |b cr |2 rdacarrier | ||
520 | |a AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP9)during the development of PDR.<p<METHODS:C57BL/6J mice were intraperitoneal injected with STZ(55 mg/kg)for 5 consecutive days, and blood glucose concentrations were measured after 7d of the injection. The diabetic mice were further housed for 3, 4, 5mo respectively after the development of diabetes. Histological evaluation of retinas was performed. The retinal vessels were detected by immunofluorescence staining with the cluster of differentiation 31(CD31). The mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 in mice retinas was detected by Real-time PCR analysis.<p<RESULTS: Retinal histological observation and CD31 staining both demonstrate that there are more vessels in diabetic mice than in normal control mice at 5mo after the development of diabetes. As compared with normal control, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased in diabetic mice at 5mo after the development of diabetes.<p<CONCLUSION: This study demonstrates that PDR is occurred at 5mo after the development of diabetes in STZ-induced diabetic mice. In addition, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased after the development of PDR. | ||
650 | 4 | |a diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9 | |
653 | 0 | |a Ophthalmology | |
700 | 0 | |a Bin Lu |e verfasserin |4 aut | |
700 | 0 | |a Chen-Yuan Gong |e verfasserin |4 aut | |
773 | 0 | 8 | |i In |t Guoji Yanke Zazhi |d Press of International Journal of Ophthalmology (IJO PRESS), 2017 |g 16(2016), 1, Seite 6 |w (DE-627)1680946854 |x 16725123 |7 nnns |
773 | 1 | 8 | |g volume:16 |g year:2016 |g number:1 |g pages:6 |
856 | 4 | 0 | |u https://doi.org/10.3980/j.issn.1672-5123.2016.1.01 |z kostenfrei |
856 | 4 | 0 | |u https://doaj.org/article/e467fda682e4428fa6f3d55ed5bbb7e7 |z kostenfrei |
856 | 4 | 0 | |u http://ies.ijo.cn/cn_publish/2016/1/201601001.pdf |z kostenfrei |
856 | 4 | 2 | |u https://doaj.org/toc/1672-5123 |y Journal toc |z kostenfrei |
856 | 4 | 2 | |u https://doaj.org/toc/1672-5123 |y Journal toc |z kostenfrei |
912 | |a GBV_USEFLAG_A | ||
912 | |a SYSFLAG_A | ||
912 | |a GBV_DOAJ | ||
912 | |a GBV_ILN_11 | ||
912 | |a GBV_ILN_2817 | ||
951 | |a AR | ||
952 | |d 16 |j 2016 |e 1 |h 6 |
author_variant |
z y y zyy b l bl c y g cyg |
---|---|
matchkey_str |
article:16725123:2016----::tetztcnnuedaeirtnptyn5mcadhepesoos |
hierarchy_sort_str |
2016 |
callnumber-subject-code |
RE |
publishDate |
2016 |
allfields |
10.3980/j.issn.1672-5123.2016.1.01 doi (DE-627)DOAJ03353909X (DE-599)DOAJe467fda682e4428fa6f3d55ed5bbb7e7 DE-627 ger DE-627 rakwb eng RE1-994 Zeng-Yang Yu verfasserin aut Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP9)during the development of PDR.<p<METHODS:C57BL/6J mice were intraperitoneal injected with STZ(55 mg/kg)for 5 consecutive days, and blood glucose concentrations were measured after 7d of the injection. The diabetic mice were further housed for 3, 4, 5mo respectively after the development of diabetes. Histological evaluation of retinas was performed. The retinal vessels were detected by immunofluorescence staining with the cluster of differentiation 31(CD31). The mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 in mice retinas was detected by Real-time PCR analysis.<p<RESULTS: Retinal histological observation and CD31 staining both demonstrate that there are more vessels in diabetic mice than in normal control mice at 5mo after the development of diabetes. As compared with normal control, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased in diabetic mice at 5mo after the development of diabetes.<p<CONCLUSION: This study demonstrates that PDR is occurred at 5mo after the development of diabetes in STZ-induced diabetic mice. In addition, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased after the development of PDR. diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9 Ophthalmology Bin Lu verfasserin aut Chen-Yuan Gong verfasserin aut In Guoji Yanke Zazhi Press of International Journal of Ophthalmology (IJO PRESS), 2017 16(2016), 1, Seite 6 (DE-627)1680946854 16725123 nnns volume:16 year:2016 number:1 pages:6 https://doi.org/10.3980/j.issn.1672-5123.2016.1.01 kostenfrei https://doaj.org/article/e467fda682e4428fa6f3d55ed5bbb7e7 kostenfrei http://ies.ijo.cn/cn_publish/2016/1/201601001.pdf kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_2817 AR 16 2016 1 6 |
spelling |
10.3980/j.issn.1672-5123.2016.1.01 doi (DE-627)DOAJ03353909X (DE-599)DOAJe467fda682e4428fa6f3d55ed5bbb7e7 DE-627 ger DE-627 rakwb eng RE1-994 Zeng-Yang Yu verfasserin aut Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP9)during the development of PDR.<p<METHODS:C57BL/6J mice were intraperitoneal injected with STZ(55 mg/kg)for 5 consecutive days, and blood glucose concentrations were measured after 7d of the injection. The diabetic mice were further housed for 3, 4, 5mo respectively after the development of diabetes. Histological evaluation of retinas was performed. The retinal vessels were detected by immunofluorescence staining with the cluster of differentiation 31(CD31). The mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 in mice retinas was detected by Real-time PCR analysis.<p<RESULTS: Retinal histological observation and CD31 staining both demonstrate that there are more vessels in diabetic mice than in normal control mice at 5mo after the development of diabetes. As compared with normal control, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased in diabetic mice at 5mo after the development of diabetes.<p<CONCLUSION: This study demonstrates that PDR is occurred at 5mo after the development of diabetes in STZ-induced diabetic mice. In addition, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased after the development of PDR. diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9 Ophthalmology Bin Lu verfasserin aut Chen-Yuan Gong verfasserin aut In Guoji Yanke Zazhi Press of International Journal of Ophthalmology (IJO PRESS), 2017 16(2016), 1, Seite 6 (DE-627)1680946854 16725123 nnns volume:16 year:2016 number:1 pages:6 https://doi.org/10.3980/j.issn.1672-5123.2016.1.01 kostenfrei https://doaj.org/article/e467fda682e4428fa6f3d55ed5bbb7e7 kostenfrei http://ies.ijo.cn/cn_publish/2016/1/201601001.pdf kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_2817 AR 16 2016 1 6 |
allfields_unstemmed |
10.3980/j.issn.1672-5123.2016.1.01 doi (DE-627)DOAJ03353909X (DE-599)DOAJe467fda682e4428fa6f3d55ed5bbb7e7 DE-627 ger DE-627 rakwb eng RE1-994 Zeng-Yang Yu verfasserin aut Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP9)during the development of PDR.<p<METHODS:C57BL/6J mice were intraperitoneal injected with STZ(55 mg/kg)for 5 consecutive days, and blood glucose concentrations were measured after 7d of the injection. The diabetic mice were further housed for 3, 4, 5mo respectively after the development of diabetes. Histological evaluation of retinas was performed. The retinal vessels were detected by immunofluorescence staining with the cluster of differentiation 31(CD31). The mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 in mice retinas was detected by Real-time PCR analysis.<p<RESULTS: Retinal histological observation and CD31 staining both demonstrate that there are more vessels in diabetic mice than in normal control mice at 5mo after the development of diabetes. As compared with normal control, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased in diabetic mice at 5mo after the development of diabetes.<p<CONCLUSION: This study demonstrates that PDR is occurred at 5mo after the development of diabetes in STZ-induced diabetic mice. In addition, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased after the development of PDR. diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9 Ophthalmology Bin Lu verfasserin aut Chen-Yuan Gong verfasserin aut In Guoji Yanke Zazhi Press of International Journal of Ophthalmology (IJO PRESS), 2017 16(2016), 1, Seite 6 (DE-627)1680946854 16725123 nnns volume:16 year:2016 number:1 pages:6 https://doi.org/10.3980/j.issn.1672-5123.2016.1.01 kostenfrei https://doaj.org/article/e467fda682e4428fa6f3d55ed5bbb7e7 kostenfrei http://ies.ijo.cn/cn_publish/2016/1/201601001.pdf kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_2817 AR 16 2016 1 6 |
allfieldsGer |
10.3980/j.issn.1672-5123.2016.1.01 doi (DE-627)DOAJ03353909X (DE-599)DOAJe467fda682e4428fa6f3d55ed5bbb7e7 DE-627 ger DE-627 rakwb eng RE1-994 Zeng-Yang Yu verfasserin aut Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP9)during the development of PDR.<p<METHODS:C57BL/6J mice were intraperitoneal injected with STZ(55 mg/kg)for 5 consecutive days, and blood glucose concentrations were measured after 7d of the injection. The diabetic mice were further housed for 3, 4, 5mo respectively after the development of diabetes. Histological evaluation of retinas was performed. The retinal vessels were detected by immunofluorescence staining with the cluster of differentiation 31(CD31). The mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 in mice retinas was detected by Real-time PCR analysis.<p<RESULTS: Retinal histological observation and CD31 staining both demonstrate that there are more vessels in diabetic mice than in normal control mice at 5mo after the development of diabetes. As compared with normal control, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased in diabetic mice at 5mo after the development of diabetes.<p<CONCLUSION: This study demonstrates that PDR is occurred at 5mo after the development of diabetes in STZ-induced diabetic mice. In addition, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased after the development of PDR. diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9 Ophthalmology Bin Lu verfasserin aut Chen-Yuan Gong verfasserin aut In Guoji Yanke Zazhi Press of International Journal of Ophthalmology (IJO PRESS), 2017 16(2016), 1, Seite 6 (DE-627)1680946854 16725123 nnns volume:16 year:2016 number:1 pages:6 https://doi.org/10.3980/j.issn.1672-5123.2016.1.01 kostenfrei https://doaj.org/article/e467fda682e4428fa6f3d55ed5bbb7e7 kostenfrei http://ies.ijo.cn/cn_publish/2016/1/201601001.pdf kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_2817 AR 16 2016 1 6 |
allfieldsSound |
10.3980/j.issn.1672-5123.2016.1.01 doi (DE-627)DOAJ03353909X (DE-599)DOAJe467fda682e4428fa6f3d55ed5bbb7e7 DE-627 ger DE-627 rakwb eng RE1-994 Zeng-Yang Yu verfasserin aut Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP9)during the development of PDR.<p<METHODS:C57BL/6J mice were intraperitoneal injected with STZ(55 mg/kg)for 5 consecutive days, and blood glucose concentrations were measured after 7d of the injection. The diabetic mice were further housed for 3, 4, 5mo respectively after the development of diabetes. Histological evaluation of retinas was performed. The retinal vessels were detected by immunofluorescence staining with the cluster of differentiation 31(CD31). The mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 in mice retinas was detected by Real-time PCR analysis.<p<RESULTS: Retinal histological observation and CD31 staining both demonstrate that there are more vessels in diabetic mice than in normal control mice at 5mo after the development of diabetes. As compared with normal control, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased in diabetic mice at 5mo after the development of diabetes.<p<CONCLUSION: This study demonstrates that PDR is occurred at 5mo after the development of diabetes in STZ-induced diabetic mice. In addition, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased after the development of PDR. diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9 Ophthalmology Bin Lu verfasserin aut Chen-Yuan Gong verfasserin aut In Guoji Yanke Zazhi Press of International Journal of Ophthalmology (IJO PRESS), 2017 16(2016), 1, Seite 6 (DE-627)1680946854 16725123 nnns volume:16 year:2016 number:1 pages:6 https://doi.org/10.3980/j.issn.1672-5123.2016.1.01 kostenfrei https://doaj.org/article/e467fda682e4428fa6f3d55ed5bbb7e7 kostenfrei http://ies.ijo.cn/cn_publish/2016/1/201601001.pdf kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei https://doaj.org/toc/1672-5123 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_2817 AR 16 2016 1 6 |
language |
English |
source |
In Guoji Yanke Zazhi 16(2016), 1, Seite 6 volume:16 year:2016 number:1 pages:6 |
sourceStr |
In Guoji Yanke Zazhi 16(2016), 1, Seite 6 volume:16 year:2016 number:1 pages:6 |
format_phy_str_mv |
Article |
institution |
findex.gbv.de |
topic_facet |
diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9 Ophthalmology |
isfreeaccess_bool |
true |
container_title |
Guoji Yanke Zazhi |
authorswithroles_txt_mv |
Zeng-Yang Yu @@aut@@ Bin Lu @@aut@@ Chen-Yuan Gong @@aut@@ |
publishDateDaySort_date |
2016-01-01T00:00:00Z |
hierarchy_top_id |
1680946854 |
id |
DOAJ03353909X |
language_de |
englisch |
fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ03353909X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230307180920.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230226s2016 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3980/j.issn.1672-5123.2016.1.01</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ03353909X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJe467fda682e4428fa6f3d55ed5bbb7e7</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">RE1-994</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Zeng-Yang Yu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2016</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP9)during the development of PDR.<p<METHODS:C57BL/6J mice were intraperitoneal injected with STZ(55 mg/kg)for 5 consecutive days, and blood glucose concentrations were measured after 7d of the injection. The diabetic mice were further housed for 3, 4, 5mo respectively after the development of diabetes. Histological evaluation of retinas was performed. The retinal vessels were detected by immunofluorescence staining with the cluster of differentiation 31(CD31). The mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 in mice retinas was detected by Real-time PCR analysis.<p<RESULTS: Retinal histological observation and CD31 staining both demonstrate that there are more vessels in diabetic mice than in normal control mice at 5mo after the development of diabetes. As compared with normal control, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased in diabetic mice at 5mo after the development of diabetes.<p<CONCLUSION: This study demonstrates that PDR is occurred at 5mo after the development of diabetes in STZ-induced diabetic mice. In addition, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased after the development of PDR.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Ophthalmology</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Bin Lu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Chen-Yuan Gong</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Guoji Yanke Zazhi</subfield><subfield code="d">Press of International Journal of Ophthalmology (IJO PRESS), 2017</subfield><subfield code="g">16(2016), 1, Seite 6</subfield><subfield code="w">(DE-627)1680946854</subfield><subfield code="x">16725123</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:16</subfield><subfield code="g">year:2016</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:6</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.3980/j.issn.1672-5123.2016.1.01</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/e467fda682e4428fa6f3d55ed5bbb7e7</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://ies.ijo.cn/cn_publish/2016/1/201601001.pdf</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1672-5123</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1672-5123</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_11</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2817</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">16</subfield><subfield code="j">2016</subfield><subfield code="e">1</subfield><subfield code="h">6</subfield></datafield></record></collection>
|
callnumber-first |
R - Medicine |
author |
Zeng-Yang Yu |
spellingShingle |
Zeng-Yang Yu misc RE1-994 misc diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9 misc Ophthalmology Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules |
authorStr |
Zeng-Yang Yu |
ppnlink_with_tag_str_mv |
@@773@@(DE-627)1680946854 |
format |
electronic Article |
delete_txt_mv |
keep |
author_role |
aut aut aut |
collection |
DOAJ |
remote_str |
true |
callnumber-label |
RE1-994 |
illustrated |
Not Illustrated |
issn |
16725123 |
topic_title |
RE1-994 Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9 |
topic |
misc RE1-994 misc diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9 misc Ophthalmology |
topic_unstemmed |
misc RE1-994 misc diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9 misc Ophthalmology |
topic_browse |
misc RE1-994 misc diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9 misc Ophthalmology |
format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
format_main_str_mv |
Text Zeitschrift/Artikel |
carriertype_str_mv |
cr |
hierarchy_parent_title |
Guoji Yanke Zazhi |
hierarchy_parent_id |
1680946854 |
hierarchy_top_title |
Guoji Yanke Zazhi |
isfreeaccess_txt |
true |
familylinks_str_mv |
(DE-627)1680946854 |
title |
Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules |
ctrlnum |
(DE-627)DOAJ03353909X (DE-599)DOAJe467fda682e4428fa6f3d55ed5bbb7e7 |
title_full |
Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules |
author_sort |
Zeng-Yang Yu |
journal |
Guoji Yanke Zazhi |
journalStr |
Guoji Yanke Zazhi |
callnumber-first-code |
R |
lang_code |
eng |
isOA_bool |
true |
recordtype |
marc |
publishDateSort |
2016 |
contenttype_str_mv |
txt |
container_start_page |
6 |
author_browse |
Zeng-Yang Yu Bin Lu Chen-Yuan Gong |
container_volume |
16 |
class |
RE1-994 |
format_se |
Elektronische Aufsätze |
author-letter |
Zeng-Yang Yu |
doi_str_mv |
10.3980/j.issn.1672-5123.2016.1.01 |
author2-role |
verfasserin |
title_sort |
streptozotocin induced diabetic retinopathy in c57 mice and the expression of some pro-angiogenic molecules |
callnumber |
RE1-994 |
title_auth |
Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules |
abstract |
AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP9)during the development of PDR.<p<METHODS:C57BL/6J mice were intraperitoneal injected with STZ(55 mg/kg)for 5 consecutive days, and blood glucose concentrations were measured after 7d of the injection. The diabetic mice were further housed for 3, 4, 5mo respectively after the development of diabetes. Histological evaluation of retinas was performed. The retinal vessels were detected by immunofluorescence staining with the cluster of differentiation 31(CD31). The mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 in mice retinas was detected by Real-time PCR analysis.<p<RESULTS: Retinal histological observation and CD31 staining both demonstrate that there are more vessels in diabetic mice than in normal control mice at 5mo after the development of diabetes. As compared with normal control, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased in diabetic mice at 5mo after the development of diabetes.<p<CONCLUSION: This study demonstrates that PDR is occurred at 5mo after the development of diabetes in STZ-induced diabetic mice. In addition, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased after the development of PDR. |
abstractGer |
AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP9)during the development of PDR.<p<METHODS:C57BL/6J mice were intraperitoneal injected with STZ(55 mg/kg)for 5 consecutive days, and blood glucose concentrations were measured after 7d of the injection. The diabetic mice were further housed for 3, 4, 5mo respectively after the development of diabetes. Histological evaluation of retinas was performed. The retinal vessels were detected by immunofluorescence staining with the cluster of differentiation 31(CD31). The mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 in mice retinas was detected by Real-time PCR analysis.<p<RESULTS: Retinal histological observation and CD31 staining both demonstrate that there are more vessels in diabetic mice than in normal control mice at 5mo after the development of diabetes. As compared with normal control, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased in diabetic mice at 5mo after the development of diabetes.<p<CONCLUSION: This study demonstrates that PDR is occurred at 5mo after the development of diabetes in STZ-induced diabetic mice. In addition, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased after the development of PDR. |
abstract_unstemmed |
AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP9)during the development of PDR.<p<METHODS:C57BL/6J mice were intraperitoneal injected with STZ(55 mg/kg)for 5 consecutive days, and blood glucose concentrations were measured after 7d of the injection. The diabetic mice were further housed for 3, 4, 5mo respectively after the development of diabetes. Histological evaluation of retinas was performed. The retinal vessels were detected by immunofluorescence staining with the cluster of differentiation 31(CD31). The mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 in mice retinas was detected by Real-time PCR analysis.<p<RESULTS: Retinal histological observation and CD31 staining both demonstrate that there are more vessels in diabetic mice than in normal control mice at 5mo after the development of diabetes. As compared with normal control, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased in diabetic mice at 5mo after the development of diabetes.<p<CONCLUSION: This study demonstrates that PDR is occurred at 5mo after the development of diabetes in STZ-induced diabetic mice. In addition, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased after the development of PDR. |
collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_2817 |
container_issue |
1 |
title_short |
Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules |
url |
https://doi.org/10.3980/j.issn.1672-5123.2016.1.01 https://doaj.org/article/e467fda682e4428fa6f3d55ed5bbb7e7 http://ies.ijo.cn/cn_publish/2016/1/201601001.pdf https://doaj.org/toc/1672-5123 |
remote_bool |
true |
author2 |
Bin Lu Chen-Yuan Gong |
author2Str |
Bin Lu Chen-Yuan Gong |
ppnlink |
1680946854 |
callnumber-subject |
RE - Ophthalmology |
mediatype_str_mv |
c |
isOA_txt |
true |
hochschulschrift_bool |
false |
doi_str |
10.3980/j.issn.1672-5123.2016.1.01 |
callnumber-a |
RE1-994 |
up_date |
2024-07-03T18:20:22.465Z |
_version_ |
1803583039702302720 |
fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ03353909X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230307180920.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230226s2016 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3980/j.issn.1672-5123.2016.1.01</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ03353909X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJe467fda682e4428fa6f3d55ed5bbb7e7</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">RE1-994</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Zeng-Yang Yu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Streptozotocin induced diabetic retinopathy in C57 mice and the expression of some pro-angiogenic molecules</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2016</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">AIM: To establish the mice model of streptozotocin(STZ)-induced proliferative diabetic retinopathy(PDR), and observe the altered expression of some pro-angiogenic molecules such as vascular endothelial growth factor(VEGF)and its receptors(VEGFR1 and VEGFR2), and matrix metalloproteinase(MMP2 and MMP9)during the development of PDR.<p<METHODS:C57BL/6J mice were intraperitoneal injected with STZ(55 mg/kg)for 5 consecutive days, and blood glucose concentrations were measured after 7d of the injection. The diabetic mice were further housed for 3, 4, 5mo respectively after the development of diabetes. Histological evaluation of retinas was performed. The retinal vessels were detected by immunofluorescence staining with the cluster of differentiation 31(CD31). The mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 in mice retinas was detected by Real-time PCR analysis.<p<RESULTS: Retinal histological observation and CD31 staining both demonstrate that there are more vessels in diabetic mice than in normal control mice at 5mo after the development of diabetes. As compared with normal control, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased in diabetic mice at 5mo after the development of diabetes.<p<CONCLUSION: This study demonstrates that PDR is occurred at 5mo after the development of diabetes in STZ-induced diabetic mice. In addition, the mRNA expression of VEGF, VEGFR1, VEGFR2, MMP2 and MMP9 are all increased after the development of PDR.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">diabetic retinopathy; VEGF; VEGFR1; VEGFR2; MMP2; MMP9</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Ophthalmology</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Bin Lu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Chen-Yuan Gong</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Guoji Yanke Zazhi</subfield><subfield code="d">Press of International Journal of Ophthalmology (IJO PRESS), 2017</subfield><subfield code="g">16(2016), 1, Seite 6</subfield><subfield code="w">(DE-627)1680946854</subfield><subfield code="x">16725123</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:16</subfield><subfield code="g">year:2016</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:6</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.3980/j.issn.1672-5123.2016.1.01</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/e467fda682e4428fa6f3d55ed5bbb7e7</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://ies.ijo.cn/cn_publish/2016/1/201601001.pdf</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1672-5123</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1672-5123</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_11</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2817</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">16</subfield><subfield code="j">2016</subfield><subfield code="e">1</subfield><subfield code="h">6</subfield></datafield></record></collection>
|
score |
7.400346 |