Antibiotic Resistance and Molecular Characterization of <i<Cronobacter sakazakii</i< Strains Isolated from Powdered Infant Formula Milk

Background: <i<Cronobacter sakazakii</i< is a new emerging foodborne bacterial pathogen associated with severe lethal diseases such as meningitis, necrotizing enterocolitis, and septicemia in infants and neonates. Powdered infant formula milk (PIFM) has been recognized as one of the main...
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Autor*in:	Babak Pakbin [verfasserIn] Wolfram Manuel Brück [verfasserIn] Samaneh Allahyari [verfasserIn] John W. A. Rossen [verfasserIn] Razzagh Mahmoudi [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	powdered infant formula milk antibiotic resistance ERIC-PCR

Übergeordnetes Werk:	In: Foods - MDPI AG, 2013, 11(2022), 8, p 1093
Übergeordnetes Werk:	volume:11 ; year:2022 ; number:8, p 1093

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/foods11081093

Katalog-ID:	DOAJ033976198

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520			\|a Background: <i<Cronobacter sakazakii</i< is a new emerging foodborne bacterial pathogen associated with severe lethal diseases such as meningitis, necrotizing enterocolitis, and septicemia in infants and neonates. Powdered infant formula milk (PIFM) has been recognized as one of the main transmission vehicles and contaminated sources of this pathogen. This study aimed to investigate the prevalence rate, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of <i<C. sakazakii</i< strains isolated from 364 PIFM samples collected from Tehran city, Iran. Methods: Culture-based methods, Kirby–Bauer disk diffusion antibiotic resistance testing, conventional Polymerase Chain Reaction (PCR), and Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) assays were used in this study to detect and characterize the <i<C. sakazakii</i< isolates. Results: We isolated 25 <i<C. sakazakii</i< strains from PIFM samples (6.86%). The isolates were highly resistant to amoxicillin-clavulanic acid, amoxicillin, ampicillin, cefoxitin, cefepime, erythromycin, ceftriaxone, ciprofloxacin, and chloramphenicol and susceptible to gentamicin, tetracycline, norfloxacin, and azithromycin antibiotics. The blaCTX-M-1 gene was detected in 96% of the isolates. The isolates were categorized into eight distinct clonal types using the ERIC-PCR method, showing a high genetic diversity among the isolates. However, there was a significant correlation between the genotypic and phenotypic antibiotic resistance properties of the isolates. Conclusions: Novel microbial surveillance systems for detecting multi-drug-resistant <i<C. sakazakii</i< are required to control the contamination of this foodborne pathogen in infant foods.
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allfields	10.3390/foods11081093 doi (DE-627)DOAJ033976198 (DE-599)DOAJ1c00c570fbda4ecbbd348bd7ef8737b0 DE-627 ger DE-627 rakwb eng TP1-1185 Babak Pakbin verfasserin aut Antibiotic Resistance and Molecular Characterization of <i<Cronobacter sakazakii</i< Strains Isolated from Powdered Infant Formula Milk 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: <i<Cronobacter sakazakii</i< is a new emerging foodborne bacterial pathogen associated with severe lethal diseases such as meningitis, necrotizing enterocolitis, and septicemia in infants and neonates. Powdered infant formula milk (PIFM) has been recognized as one of the main transmission vehicles and contaminated sources of this pathogen. This study aimed to investigate the prevalence rate, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of <i<C. sakazakii</i< strains isolated from 364 PIFM samples collected from Tehran city, Iran. Methods: Culture-based methods, Kirby–Bauer disk diffusion antibiotic resistance testing, conventional Polymerase Chain Reaction (PCR), and Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) assays were used in this study to detect and characterize the <i<C. sakazakii</i< isolates. Results: We isolated 25 <i<C. sakazakii</i< strains from PIFM samples (6.86%). The isolates were highly resistant to amoxicillin-clavulanic acid, amoxicillin, ampicillin, cefoxitin, cefepime, erythromycin, ceftriaxone, ciprofloxacin, and chloramphenicol and susceptible to gentamicin, tetracycline, norfloxacin, and azithromycin antibiotics. The blaCTX-M-1 gene was detected in 96% of the isolates. The isolates were categorized into eight distinct clonal types using the ERIC-PCR method, showing a high genetic diversity among the isolates. However, there was a significant correlation between the genotypic and phenotypic antibiotic resistance properties of the isolates. Conclusions: Novel microbial surveillance systems for detecting multi-drug-resistant <i<C. sakazakii</i< are required to control the contamination of this foodborne pathogen in infant foods. <i<Cronobacter sakazakii</i< powdered infant formula milk antibiotic resistance ERIC-PCR Chemical technology Wolfram Manuel Brück verfasserin aut Samaneh Allahyari verfasserin aut John W. A. Rossen verfasserin aut Razzagh Mahmoudi verfasserin aut In Foods MDPI AG, 2013 11(2022), 8, p 1093 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:11 year:2022 number:8, p 1093 https://doi.org/10.3390/foods11081093 kostenfrei https://doaj.org/article/1c00c570fbda4ecbbd348bd7ef8737b0 kostenfrei https://www.mdpi.com/2304-8158/11/8/1093 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 8, p 1093
spelling	10.3390/foods11081093 doi (DE-627)DOAJ033976198 (DE-599)DOAJ1c00c570fbda4ecbbd348bd7ef8737b0 DE-627 ger DE-627 rakwb eng TP1-1185 Babak Pakbin verfasserin aut Antibiotic Resistance and Molecular Characterization of <i<Cronobacter sakazakii</i< Strains Isolated from Powdered Infant Formula Milk 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: <i<Cronobacter sakazakii</i< is a new emerging foodborne bacterial pathogen associated with severe lethal diseases such as meningitis, necrotizing enterocolitis, and septicemia in infants and neonates. Powdered infant formula milk (PIFM) has been recognized as one of the main transmission vehicles and contaminated sources of this pathogen. This study aimed to investigate the prevalence rate, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of <i<C. sakazakii</i< strains isolated from 364 PIFM samples collected from Tehran city, Iran. Methods: Culture-based methods, Kirby–Bauer disk diffusion antibiotic resistance testing, conventional Polymerase Chain Reaction (PCR), and Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) assays were used in this study to detect and characterize the <i<C. sakazakii</i< isolates. Results: We isolated 25 <i<C. sakazakii</i< strains from PIFM samples (6.86%). The isolates were highly resistant to amoxicillin-clavulanic acid, amoxicillin, ampicillin, cefoxitin, cefepime, erythromycin, ceftriaxone, ciprofloxacin, and chloramphenicol and susceptible to gentamicin, tetracycline, norfloxacin, and azithromycin antibiotics. The blaCTX-M-1 gene was detected in 96% of the isolates. The isolates were categorized into eight distinct clonal types using the ERIC-PCR method, showing a high genetic diversity among the isolates. However, there was a significant correlation between the genotypic and phenotypic antibiotic resistance properties of the isolates. Conclusions: Novel microbial surveillance systems for detecting multi-drug-resistant <i<C. sakazakii</i< are required to control the contamination of this foodborne pathogen in infant foods. <i<Cronobacter sakazakii</i< powdered infant formula milk antibiotic resistance ERIC-PCR Chemical technology Wolfram Manuel Brück verfasserin aut Samaneh Allahyari verfasserin aut John W. A. Rossen verfasserin aut Razzagh Mahmoudi verfasserin aut In Foods MDPI AG, 2013 11(2022), 8, p 1093 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:11 year:2022 number:8, p 1093 https://doi.org/10.3390/foods11081093 kostenfrei https://doaj.org/article/1c00c570fbda4ecbbd348bd7ef8737b0 kostenfrei https://www.mdpi.com/2304-8158/11/8/1093 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 8, p 1093
allfields_unstemmed	10.3390/foods11081093 doi (DE-627)DOAJ033976198 (DE-599)DOAJ1c00c570fbda4ecbbd348bd7ef8737b0 DE-627 ger DE-627 rakwb eng TP1-1185 Babak Pakbin verfasserin aut Antibiotic Resistance and Molecular Characterization of <i<Cronobacter sakazakii</i< Strains Isolated from Powdered Infant Formula Milk 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: <i<Cronobacter sakazakii</i< is a new emerging foodborne bacterial pathogen associated with severe lethal diseases such as meningitis, necrotizing enterocolitis, and septicemia in infants and neonates. Powdered infant formula milk (PIFM) has been recognized as one of the main transmission vehicles and contaminated sources of this pathogen. This study aimed to investigate the prevalence rate, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of <i<C. sakazakii</i< strains isolated from 364 PIFM samples collected from Tehran city, Iran. Methods: Culture-based methods, Kirby–Bauer disk diffusion antibiotic resistance testing, conventional Polymerase Chain Reaction (PCR), and Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) assays were used in this study to detect and characterize the <i<C. sakazakii</i< isolates. Results: We isolated 25 <i<C. sakazakii</i< strains from PIFM samples (6.86%). The isolates were highly resistant to amoxicillin-clavulanic acid, amoxicillin, ampicillin, cefoxitin, cefepime, erythromycin, ceftriaxone, ciprofloxacin, and chloramphenicol and susceptible to gentamicin, tetracycline, norfloxacin, and azithromycin antibiotics. The blaCTX-M-1 gene was detected in 96% of the isolates. The isolates were categorized into eight distinct clonal types using the ERIC-PCR method, showing a high genetic diversity among the isolates. However, there was a significant correlation between the genotypic and phenotypic antibiotic resistance properties of the isolates. Conclusions: Novel microbial surveillance systems for detecting multi-drug-resistant <i<C. sakazakii</i< are required to control the contamination of this foodborne pathogen in infant foods. <i<Cronobacter sakazakii</i< powdered infant formula milk antibiotic resistance ERIC-PCR Chemical technology Wolfram Manuel Brück verfasserin aut Samaneh Allahyari verfasserin aut John W. A. Rossen verfasserin aut Razzagh Mahmoudi verfasserin aut In Foods MDPI AG, 2013 11(2022), 8, p 1093 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:11 year:2022 number:8, p 1093 https://doi.org/10.3390/foods11081093 kostenfrei https://doaj.org/article/1c00c570fbda4ecbbd348bd7ef8737b0 kostenfrei https://www.mdpi.com/2304-8158/11/8/1093 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 8, p 1093
allfieldsGer	10.3390/foods11081093 doi (DE-627)DOAJ033976198 (DE-599)DOAJ1c00c570fbda4ecbbd348bd7ef8737b0 DE-627 ger DE-627 rakwb eng TP1-1185 Babak Pakbin verfasserin aut Antibiotic Resistance and Molecular Characterization of <i<Cronobacter sakazakii</i< Strains Isolated from Powdered Infant Formula Milk 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: <i<Cronobacter sakazakii</i< is a new emerging foodborne bacterial pathogen associated with severe lethal diseases such as meningitis, necrotizing enterocolitis, and septicemia in infants and neonates. Powdered infant formula milk (PIFM) has been recognized as one of the main transmission vehicles and contaminated sources of this pathogen. This study aimed to investigate the prevalence rate, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of <i<C. sakazakii</i< strains isolated from 364 PIFM samples collected from Tehran city, Iran. Methods: Culture-based methods, Kirby–Bauer disk diffusion antibiotic resistance testing, conventional Polymerase Chain Reaction (PCR), and Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) assays were used in this study to detect and characterize the <i<C. sakazakii</i< isolates. Results: We isolated 25 <i<C. sakazakii</i< strains from PIFM samples (6.86%). The isolates were highly resistant to amoxicillin-clavulanic acid, amoxicillin, ampicillin, cefoxitin, cefepime, erythromycin, ceftriaxone, ciprofloxacin, and chloramphenicol and susceptible to gentamicin, tetracycline, norfloxacin, and azithromycin antibiotics. The blaCTX-M-1 gene was detected in 96% of the isolates. The isolates were categorized into eight distinct clonal types using the ERIC-PCR method, showing a high genetic diversity among the isolates. However, there was a significant correlation between the genotypic and phenotypic antibiotic resistance properties of the isolates. Conclusions: Novel microbial surveillance systems for detecting multi-drug-resistant <i<C. sakazakii</i< are required to control the contamination of this foodborne pathogen in infant foods. <i<Cronobacter sakazakii</i< powdered infant formula milk antibiotic resistance ERIC-PCR Chemical technology Wolfram Manuel Brück verfasserin aut Samaneh Allahyari verfasserin aut John W. A. Rossen verfasserin aut Razzagh Mahmoudi verfasserin aut In Foods MDPI AG, 2013 11(2022), 8, p 1093 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:11 year:2022 number:8, p 1093 https://doi.org/10.3390/foods11081093 kostenfrei https://doaj.org/article/1c00c570fbda4ecbbd348bd7ef8737b0 kostenfrei https://www.mdpi.com/2304-8158/11/8/1093 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 8, p 1093
allfieldsSound	10.3390/foods11081093 doi (DE-627)DOAJ033976198 (DE-599)DOAJ1c00c570fbda4ecbbd348bd7ef8737b0 DE-627 ger DE-627 rakwb eng TP1-1185 Babak Pakbin verfasserin aut Antibiotic Resistance and Molecular Characterization of <i<Cronobacter sakazakii</i< Strains Isolated from Powdered Infant Formula Milk 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: <i<Cronobacter sakazakii</i< is a new emerging foodborne bacterial pathogen associated with severe lethal diseases such as meningitis, necrotizing enterocolitis, and septicemia in infants and neonates. Powdered infant formula milk (PIFM) has been recognized as one of the main transmission vehicles and contaminated sources of this pathogen. This study aimed to investigate the prevalence rate, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of <i<C. sakazakii</i< strains isolated from 364 PIFM samples collected from Tehran city, Iran. Methods: Culture-based methods, Kirby–Bauer disk diffusion antibiotic resistance testing, conventional Polymerase Chain Reaction (PCR), and Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) assays were used in this study to detect and characterize the <i<C. sakazakii</i< isolates. Results: We isolated 25 <i<C. sakazakii</i< strains from PIFM samples (6.86%). The isolates were highly resistant to amoxicillin-clavulanic acid, amoxicillin, ampicillin, cefoxitin, cefepime, erythromycin, ceftriaxone, ciprofloxacin, and chloramphenicol and susceptible to gentamicin, tetracycline, norfloxacin, and azithromycin antibiotics. The blaCTX-M-1 gene was detected in 96% of the isolates. The isolates were categorized into eight distinct clonal types using the ERIC-PCR method, showing a high genetic diversity among the isolates. However, there was a significant correlation between the genotypic and phenotypic antibiotic resistance properties of the isolates. Conclusions: Novel microbial surveillance systems for detecting multi-drug-resistant <i<C. sakazakii</i< are required to control the contamination of this foodborne pathogen in infant foods. <i<Cronobacter sakazakii</i< powdered infant formula milk antibiotic resistance ERIC-PCR Chemical technology Wolfram Manuel Brück verfasserin aut Samaneh Allahyari verfasserin aut John W. A. Rossen verfasserin aut Razzagh Mahmoudi verfasserin aut In Foods MDPI AG, 2013 11(2022), 8, p 1093 (DE-627)737287632 (DE-600)2704223-6 23048158 nnns volume:11 year:2022 number:8, p 1093 https://doi.org/10.3390/foods11081093 kostenfrei https://doaj.org/article/1c00c570fbda4ecbbd348bd7ef8737b0 kostenfrei https://www.mdpi.com/2304-8158/11/8/1093 kostenfrei https://doaj.org/toc/2304-8158 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 8, p 1093
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Powdered infant formula milk (PIFM) has been recognized as one of the main transmission vehicles and contaminated sources of this pathogen. This study aimed to investigate the prevalence rate, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of <i<C. sakazakii</i< strains isolated from 364 PIFM samples collected from Tehran city, Iran. Methods: Culture-based methods, Kirby–Bauer disk diffusion antibiotic resistance testing, conventional Polymerase Chain Reaction (PCR), and Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) assays were used in this study to detect and characterize the <i<C. sakazakii</i< isolates. Results: We isolated 25 <i<C. sakazakii</i< strains from PIFM samples (6.86%). The isolates were highly resistant to amoxicillin-clavulanic acid, amoxicillin, ampicillin, cefoxitin, cefepime, erythromycin, ceftriaxone, ciprofloxacin, and chloramphenicol and susceptible to gentamicin, tetracycline, norfloxacin, and azithromycin antibiotics. The blaCTX-M-1 gene was detected in 96% of the isolates. The isolates were categorized into eight distinct clonal types using the ERIC-PCR method, showing a high genetic diversity among the isolates. However, there was a significant correlation between the genotypic and phenotypic antibiotic resistance properties of the isolates. Conclusions: Novel microbial surveillance systems for detecting multi-drug-resistant <i<C. sakazakii</i< are required to control the contamination of this foodborne pathogen in infant foods.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<Cronobacter sakazakii</i<</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">powdered infant formula milk</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">antibiotic resistance</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">ERIC-PCR</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Chemical technology</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Wolfram Manuel Brück</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Samaneh Allahyari</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">John W. 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callnumber-first	T - Technology
author	Babak Pakbin
spellingShingle	Babak Pakbin misc TP1-1185 misc <i<Cronobacter sakazakii</i< misc powdered infant formula milk misc antibiotic resistance misc ERIC-PCR misc Chemical technology Antibiotic Resistance and Molecular Characterization of <i<Cronobacter sakazakii</i< Strains Isolated from Powdered Infant Formula Milk
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topic_title	TP1-1185 Antibiotic Resistance and Molecular Characterization of <i<Cronobacter sakazakii</i< Strains Isolated from Powdered Infant Formula Milk <i<Cronobacter sakazakii</i< powdered infant formula milk antibiotic resistance ERIC-PCR
topic	misc TP1-1185 misc <i<Cronobacter sakazakii</i< misc powdered infant formula milk misc antibiotic resistance misc ERIC-PCR misc Chemical technology
topic_unstemmed	misc TP1-1185 misc <i<Cronobacter sakazakii</i< misc powdered infant formula milk misc antibiotic resistance misc ERIC-PCR misc Chemical technology
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title	Antibiotic Resistance and Molecular Characterization of <i<Cronobacter sakazakii</i< Strains Isolated from Powdered Infant Formula Milk
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title_full	Antibiotic Resistance and Molecular Characterization of <i<Cronobacter sakazakii</i< Strains Isolated from Powdered Infant Formula Milk
author_sort	Babak Pakbin
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author_browse	Babak Pakbin Wolfram Manuel Brück Samaneh Allahyari John W. A. Rossen Razzagh Mahmoudi
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title_sort	antibiotic resistance and molecular characterization of <i<cronobacter sakazakii</i< strains isolated from powdered infant formula milk
callnumber	TP1-1185
title_auth	Antibiotic Resistance and Molecular Characterization of <i<Cronobacter sakazakii</i< Strains Isolated from Powdered Infant Formula Milk
abstract	Background: <i<Cronobacter sakazakii</i< is a new emerging foodborne bacterial pathogen associated with severe lethal diseases such as meningitis, necrotizing enterocolitis, and septicemia in infants and neonates. Powdered infant formula milk (PIFM) has been recognized as one of the main transmission vehicles and contaminated sources of this pathogen. This study aimed to investigate the prevalence rate, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of <i<C. sakazakii</i< strains isolated from 364 PIFM samples collected from Tehran city, Iran. Methods: Culture-based methods, Kirby–Bauer disk diffusion antibiotic resistance testing, conventional Polymerase Chain Reaction (PCR), and Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) assays were used in this study to detect and characterize the <i<C. sakazakii</i< isolates. Results: We isolated 25 <i<C. sakazakii</i< strains from PIFM samples (6.86%). The isolates were highly resistant to amoxicillin-clavulanic acid, amoxicillin, ampicillin, cefoxitin, cefepime, erythromycin, ceftriaxone, ciprofloxacin, and chloramphenicol and susceptible to gentamicin, tetracycline, norfloxacin, and azithromycin antibiotics. The blaCTX-M-1 gene was detected in 96% of the isolates. The isolates were categorized into eight distinct clonal types using the ERIC-PCR method, showing a high genetic diversity among the isolates. However, there was a significant correlation between the genotypic and phenotypic antibiotic resistance properties of the isolates. Conclusions: Novel microbial surveillance systems for detecting multi-drug-resistant <i<C. sakazakii</i< are required to control the contamination of this foodborne pathogen in infant foods.
abstractGer	Background: <i<Cronobacter sakazakii</i< is a new emerging foodborne bacterial pathogen associated with severe lethal diseases such as meningitis, necrotizing enterocolitis, and septicemia in infants and neonates. Powdered infant formula milk (PIFM) has been recognized as one of the main transmission vehicles and contaminated sources of this pathogen. This study aimed to investigate the prevalence rate, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of <i<C. sakazakii</i< strains isolated from 364 PIFM samples collected from Tehran city, Iran. Methods: Culture-based methods, Kirby–Bauer disk diffusion antibiotic resistance testing, conventional Polymerase Chain Reaction (PCR), and Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) assays were used in this study to detect and characterize the <i<C. sakazakii</i< isolates. Results: We isolated 25 <i<C. sakazakii</i< strains from PIFM samples (6.86%). The isolates were highly resistant to amoxicillin-clavulanic acid, amoxicillin, ampicillin, cefoxitin, cefepime, erythromycin, ceftriaxone, ciprofloxacin, and chloramphenicol and susceptible to gentamicin, tetracycline, norfloxacin, and azithromycin antibiotics. The blaCTX-M-1 gene was detected in 96% of the isolates. The isolates were categorized into eight distinct clonal types using the ERIC-PCR method, showing a high genetic diversity among the isolates. However, there was a significant correlation between the genotypic and phenotypic antibiotic resistance properties of the isolates. Conclusions: Novel microbial surveillance systems for detecting multi-drug-resistant <i<C. sakazakii</i< are required to control the contamination of this foodborne pathogen in infant foods.
abstract_unstemmed	Background: <i<Cronobacter sakazakii</i< is a new emerging foodborne bacterial pathogen associated with severe lethal diseases such as meningitis, necrotizing enterocolitis, and septicemia in infants and neonates. Powdered infant formula milk (PIFM) has been recognized as one of the main transmission vehicles and contaminated sources of this pathogen. This study aimed to investigate the prevalence rate, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of <i<C. sakazakii</i< strains isolated from 364 PIFM samples collected from Tehran city, Iran. Methods: Culture-based methods, Kirby–Bauer disk diffusion antibiotic resistance testing, conventional Polymerase Chain Reaction (PCR), and Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) assays were used in this study to detect and characterize the <i<C. sakazakii</i< isolates. Results: We isolated 25 <i<C. sakazakii</i< strains from PIFM samples (6.86%). The isolates were highly resistant to amoxicillin-clavulanic acid, amoxicillin, ampicillin, cefoxitin, cefepime, erythromycin, ceftriaxone, ciprofloxacin, and chloramphenicol and susceptible to gentamicin, tetracycline, norfloxacin, and azithromycin antibiotics. The blaCTX-M-1 gene was detected in 96% of the isolates. The isolates were categorized into eight distinct clonal types using the ERIC-PCR method, showing a high genetic diversity among the isolates. However, there was a significant correlation between the genotypic and phenotypic antibiotic resistance properties of the isolates. Conclusions: Novel microbial surveillance systems for detecting multi-drug-resistant <i<C. sakazakii</i< are required to control the contamination of this foodborne pathogen in infant foods.
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title_short	Antibiotic Resistance and Molecular Characterization of <i<Cronobacter sakazakii</i< Strains Isolated from Powdered Infant Formula Milk
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up_date	2024-07-03T20:42:06.742Z
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Powdered infant formula milk (PIFM) has been recognized as one of the main transmission vehicles and contaminated sources of this pathogen. This study aimed to investigate the prevalence rate, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of <i<C. sakazakii</i< strains isolated from 364 PIFM samples collected from Tehran city, Iran. Methods: Culture-based methods, Kirby–Bauer disk diffusion antibiotic resistance testing, conventional Polymerase Chain Reaction (PCR), and Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) assays were used in this study to detect and characterize the <i<C. sakazakii</i< isolates. Results: We isolated 25 <i<C. sakazakii</i< strains from PIFM samples (6.86%). The isolates were highly resistant to amoxicillin-clavulanic acid, amoxicillin, ampicillin, cefoxitin, cefepime, erythromycin, ceftriaxone, ciprofloxacin, and chloramphenicol and susceptible to gentamicin, tetracycline, norfloxacin, and azithromycin antibiotics. The blaCTX-M-1 gene was detected in 96% of the isolates. The isolates were categorized into eight distinct clonal types using the ERIC-PCR method, showing a high genetic diversity among the isolates. However, there was a significant correlation between the genotypic and phenotypic antibiotic resistance properties of the isolates. 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Antibiotic Resistance and Molecular Characterization of <i<Cronobacter sakazakii</i< Strains Isolated from Powdered Infant Formula Milk

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