Cytology technique: development of a simple spot method for cultured cell suspension

<p<<strong<Aim</strong< To develop a simple spot method to attach cultured cells in suspension on to a glass slide.</p<<p<<strong<Methods</strong< We compared three approaches using both conventional and special glass slide (Shandon-Polysin), either without...
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Autor*in:	Jeanne A. Pawitan [verfasserIn] Lia Damayanti [verfasserIn] Arleni Arleni [verfasserIn] Ni M. Swantari [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2010

Übergeordnetes Werk:	In: Medical Journal of Indonesia - Faculty of Medicine Universitas Indonesia, 2013, 19(2010), 1, Seite 26-31
Übergeordnetes Werk:	volume:19 ; year:2010 ; number:1 ; pages:26-31

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.13181/mji.v19i1.378

Katalog-ID:	DOAJ034222871

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520			\|a <p<<strong<Aim</strong< To develop a simple spot method to attach cultured cells in suspension on to a glass slide.</p<<p<<strong<Methods</strong< We compared three approaches using both conventional and special glass slide (Shandon-Polysin), either without additional fetal bovine serum (FBS), or with addition of 3 or 10 μl of FBS to a 20 μl sample (altogether there were six approaches). The slides were examined qualitatively for the background color, boundary color and intactness, and whether there were folded and detached parts. Further, for each slide, the attached intact cells were counted, and the percentage of attached intact cells per number of spotted cells was calculated. The difference in attach intact cells between different approaches was analyzed by ANOVA using SPSS 13.0 for windows.</p<<p<<strong<Results</strong< There were no significant difference in the percentage of attached intact cells between the six approaches (P= 0.804), though the approach using special glass slide without additional FBS (FBS final concentration 5%) yield the highest percentage of attached intact cells, showed clean background without folded parts.</p<<p<<strong<Conclusions</strong< We have developed a simple spot method for cultured cell suspension, and the best approach to make spot specimen is using special glass slide with 5% FBS in the cell suspension. <em<<strong<(Med J Indones 2010; 19:26-31)</strong<</em<</p<<p<<strong<Keywords:</strong< <em<spot specimen, special glass slide, fetal bovine serum</em<</p<
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spelling	10.13181/mji.v19i1.378 doi (DE-627)DOAJ034222871 (DE-599)DOAJb642a5dc274843f8a538b0ce59d29a49 DE-627 ger DE-627 rakwb eng R5-920 Jeanne A. Pawitan verfasserin aut Cytology technique: development of a simple spot method for cultured cell suspension 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<strong<Aim</strong< To develop a simple spot method to attach cultured cells in suspension on to a glass slide.</p<<p<<strong<Methods</strong< We compared three approaches using both conventional and special glass slide (Shandon-Polysin), either without additional fetal bovine serum (FBS), or with addition of 3 or 10 μl of FBS to a 20 μl sample (altogether there were six approaches). The slides were examined qualitatively for the background color, boundary color and intactness, and whether there were folded and detached parts. Further, for each slide, the attached intact cells were counted, and the percentage of attached intact cells per number of spotted cells was calculated. The difference in attach intact cells between different approaches was analyzed by ANOVA using SPSS 13.0 for windows.</p<<p<<strong<Results</strong< There were no significant difference in the percentage of attached intact cells between the six approaches (P= 0.804), though the approach using special glass slide without additional FBS (FBS final concentration 5%) yield the highest percentage of attached intact cells, showed clean background without folded parts.</p<<p<<strong<Conclusions</strong< We have developed a simple spot method for cultured cell suspension, and the best approach to make spot specimen is using special glass slide with 5% FBS in the cell suspension. <em<<strong<(Med J Indones 2010; 19:26-31)</strong<</em<</p<<p<<strong<Keywords:</strong< <em<spot specimen, special glass slide, fetal bovine serum</em<</p< Medicine (General) Lia Damayanti verfasserin aut Arleni Arleni verfasserin aut Ni M. Swantari verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 19(2010), 1, Seite 26-31 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:19 year:2010 number:1 pages:26-31 https://doi.org/10.13181/mji.v19i1.378 kostenfrei https://doaj.org/article/b642a5dc274843f8a538b0ce59d29a49 kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/378 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2010 1 26-31
allfields_unstemmed	10.13181/mji.v19i1.378 doi (DE-627)DOAJ034222871 (DE-599)DOAJb642a5dc274843f8a538b0ce59d29a49 DE-627 ger DE-627 rakwb eng R5-920 Jeanne A. Pawitan verfasserin aut Cytology technique: development of a simple spot method for cultured cell suspension 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<strong<Aim</strong< To develop a simple spot method to attach cultured cells in suspension on to a glass slide.</p<<p<<strong<Methods</strong< We compared three approaches using both conventional and special glass slide (Shandon-Polysin), either without additional fetal bovine serum (FBS), or with addition of 3 or 10 μl of FBS to a 20 μl sample (altogether there were six approaches). The slides were examined qualitatively for the background color, boundary color and intactness, and whether there were folded and detached parts. Further, for each slide, the attached intact cells were counted, and the percentage of attached intact cells per number of spotted cells was calculated. The difference in attach intact cells between different approaches was analyzed by ANOVA using SPSS 13.0 for windows.</p<<p<<strong<Results</strong< There were no significant difference in the percentage of attached intact cells between the six approaches (P= 0.804), though the approach using special glass slide without additional FBS (FBS final concentration 5%) yield the highest percentage of attached intact cells, showed clean background without folded parts.</p<<p<<strong<Conclusions</strong< We have developed a simple spot method for cultured cell suspension, and the best approach to make spot specimen is using special glass slide with 5% FBS in the cell suspension. <em<<strong<(Med J Indones 2010; 19:26-31)</strong<</em<</p<<p<<strong<Keywords:</strong< <em<spot specimen, special glass slide, fetal bovine serum</em<</p< Medicine (General) Lia Damayanti verfasserin aut Arleni Arleni verfasserin aut Ni M. Swantari verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 19(2010), 1, Seite 26-31 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:19 year:2010 number:1 pages:26-31 https://doi.org/10.13181/mji.v19i1.378 kostenfrei https://doaj.org/article/b642a5dc274843f8a538b0ce59d29a49 kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/378 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2010 1 26-31
allfieldsGer	10.13181/mji.v19i1.378 doi (DE-627)DOAJ034222871 (DE-599)DOAJb642a5dc274843f8a538b0ce59d29a49 DE-627 ger DE-627 rakwb eng R5-920 Jeanne A. Pawitan verfasserin aut Cytology technique: development of a simple spot method for cultured cell suspension 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<strong<Aim</strong< To develop a simple spot method to attach cultured cells in suspension on to a glass slide.</p<<p<<strong<Methods</strong< We compared three approaches using both conventional and special glass slide (Shandon-Polysin), either without additional fetal bovine serum (FBS), or with addition of 3 or 10 μl of FBS to a 20 μl sample (altogether there were six approaches). The slides were examined qualitatively for the background color, boundary color and intactness, and whether there were folded and detached parts. Further, for each slide, the attached intact cells were counted, and the percentage of attached intact cells per number of spotted cells was calculated. The difference in attach intact cells between different approaches was analyzed by ANOVA using SPSS 13.0 for windows.</p<<p<<strong<Results</strong< There were no significant difference in the percentage of attached intact cells between the six approaches (P= 0.804), though the approach using special glass slide without additional FBS (FBS final concentration 5%) yield the highest percentage of attached intact cells, showed clean background without folded parts.</p<<p<<strong<Conclusions</strong< We have developed a simple spot method for cultured cell suspension, and the best approach to make spot specimen is using special glass slide with 5% FBS in the cell suspension. <em<<strong<(Med J Indones 2010; 19:26-31)</strong<</em<</p<<p<<strong<Keywords:</strong< <em<spot specimen, special glass slide, fetal bovine serum</em<</p< Medicine (General) Lia Damayanti verfasserin aut Arleni Arleni verfasserin aut Ni M. Swantari verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 19(2010), 1, Seite 26-31 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:19 year:2010 number:1 pages:26-31 https://doi.org/10.13181/mji.v19i1.378 kostenfrei https://doaj.org/article/b642a5dc274843f8a538b0ce59d29a49 kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/378 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2010 1 26-31
allfieldsSound	10.13181/mji.v19i1.378 doi (DE-627)DOAJ034222871 (DE-599)DOAJb642a5dc274843f8a538b0ce59d29a49 DE-627 ger DE-627 rakwb eng R5-920 Jeanne A. Pawitan verfasserin aut Cytology technique: development of a simple spot method for cultured cell suspension 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<strong<Aim</strong< To develop a simple spot method to attach cultured cells in suspension on to a glass slide.</p<<p<<strong<Methods</strong< We compared three approaches using both conventional and special glass slide (Shandon-Polysin), either without additional fetal bovine serum (FBS), or with addition of 3 or 10 μl of FBS to a 20 μl sample (altogether there were six approaches). The slides were examined qualitatively for the background color, boundary color and intactness, and whether there were folded and detached parts. Further, for each slide, the attached intact cells were counted, and the percentage of attached intact cells per number of spotted cells was calculated. The difference in attach intact cells between different approaches was analyzed by ANOVA using SPSS 13.0 for windows.</p<<p<<strong<Results</strong< There were no significant difference in the percentage of attached intact cells between the six approaches (P= 0.804), though the approach using special glass slide without additional FBS (FBS final concentration 5%) yield the highest percentage of attached intact cells, showed clean background without folded parts.</p<<p<<strong<Conclusions</strong< We have developed a simple spot method for cultured cell suspension, and the best approach to make spot specimen is using special glass slide with 5% FBS in the cell suspension. <em<<strong<(Med J Indones 2010; 19:26-31)</strong<</em<</p<<p<<strong<Keywords:</strong< <em<spot specimen, special glass slide, fetal bovine serum</em<</p< Medicine (General) Lia Damayanti verfasserin aut Arleni Arleni verfasserin aut Ni M. Swantari verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 19(2010), 1, Seite 26-31 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:19 year:2010 number:1 pages:26-31 https://doi.org/10.13181/mji.v19i1.378 kostenfrei https://doaj.org/article/b642a5dc274843f8a538b0ce59d29a49 kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/378 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2010 1 26-31
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Pawitan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Cytology technique: development of a simple spot method for cultured cell suspension</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2010</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a"><p<<strong<Aim</strong< To develop a simple spot method to attach cultured cells in suspension on to a glass slide.</p<<p<<strong<Methods</strong< We compared three approaches using both conventional and special glass slide (Shandon-Polysin), either without additional fetal bovine serum (FBS), or with addition of 3 or 10 μl of FBS to a 20 μl sample (altogether there were six approaches). 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callnumber-first	R - Medicine
author	Jeanne A. Pawitan
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topic_title	R5-920 Cytology technique: development of a simple spot method for cultured cell suspension
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title	Cytology technique: development of a simple spot method for cultured cell suspension
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title_full	Cytology technique: development of a simple spot method for cultured cell suspension
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author_browse	Jeanne A. Pawitan Lia Damayanti Arleni Arleni Ni M. Swantari
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author-letter	Jeanne A. Pawitan
doi_str_mv	10.13181/mji.v19i1.378
author2-role	verfasserin
title_sort	cytology technique: development of a simple spot method for cultured cell suspension
callnumber	R5-920
title_auth	Cytology technique: development of a simple spot method for cultured cell suspension
abstract	<p<<strong<Aim</strong< To develop a simple spot method to attach cultured cells in suspension on to a glass slide.</p<<p<<strong<Methods</strong< We compared three approaches using both conventional and special glass slide (Shandon-Polysin), either without additional fetal bovine serum (FBS), or with addition of 3 or 10 μl of FBS to a 20 μl sample (altogether there were six approaches). The slides were examined qualitatively for the background color, boundary color and intactness, and whether there were folded and detached parts. Further, for each slide, the attached intact cells were counted, and the percentage of attached intact cells per number of spotted cells was calculated. The difference in attach intact cells between different approaches was analyzed by ANOVA using SPSS 13.0 for windows.</p<<p<<strong<Results</strong< There were no significant difference in the percentage of attached intact cells between the six approaches (P= 0.804), though the approach using special glass slide without additional FBS (FBS final concentration 5%) yield the highest percentage of attached intact cells, showed clean background without folded parts.</p<<p<<strong<Conclusions</strong< We have developed a simple spot method for cultured cell suspension, and the best approach to make spot specimen is using special glass slide with 5% FBS in the cell suspension. <em<<strong<(Med J Indones 2010; 19:26-31)</strong<</em<</p<<p<<strong<Keywords:</strong< <em<spot specimen, special glass slide, fetal bovine serum</em<</p<
abstractGer	<p<<strong<Aim</strong< To develop a simple spot method to attach cultured cells in suspension on to a glass slide.</p<<p<<strong<Methods</strong< We compared three approaches using both conventional and special glass slide (Shandon-Polysin), either without additional fetal bovine serum (FBS), or with addition of 3 or 10 μl of FBS to a 20 μl sample (altogether there were six approaches). The slides were examined qualitatively for the background color, boundary color and intactness, and whether there were folded and detached parts. Further, for each slide, the attached intact cells were counted, and the percentage of attached intact cells per number of spotted cells was calculated. The difference in attach intact cells between different approaches was analyzed by ANOVA using SPSS 13.0 for windows.</p<<p<<strong<Results</strong< There were no significant difference in the percentage of attached intact cells between the six approaches (P= 0.804), though the approach using special glass slide without additional FBS (FBS final concentration 5%) yield the highest percentage of attached intact cells, showed clean background without folded parts.</p<<p<<strong<Conclusions</strong< We have developed a simple spot method for cultured cell suspension, and the best approach to make spot specimen is using special glass slide with 5% FBS in the cell suspension. <em<<strong<(Med J Indones 2010; 19:26-31)</strong<</em<</p<<p<<strong<Keywords:</strong< <em<spot specimen, special glass slide, fetal bovine serum</em<</p<
abstract_unstemmed	<p<<strong<Aim</strong< To develop a simple spot method to attach cultured cells in suspension on to a glass slide.</p<<p<<strong<Methods</strong< We compared three approaches using both conventional and special glass slide (Shandon-Polysin), either without additional fetal bovine serum (FBS), or with addition of 3 or 10 μl of FBS to a 20 μl sample (altogether there were six approaches). The slides were examined qualitatively for the background color, boundary color and intactness, and whether there were folded and detached parts. Further, for each slide, the attached intact cells were counted, and the percentage of attached intact cells per number of spotted cells was calculated. The difference in attach intact cells between different approaches was analyzed by ANOVA using SPSS 13.0 for windows.</p<<p<<strong<Results</strong< There were no significant difference in the percentage of attached intact cells between the six approaches (P= 0.804), though the approach using special glass slide without additional FBS (FBS final concentration 5%) yield the highest percentage of attached intact cells, showed clean background without folded parts.</p<<p<<strong<Conclusions</strong< We have developed a simple spot method for cultured cell suspension, and the best approach to make spot specimen is using special glass slide with 5% FBS in the cell suspension. <em<<strong<(Med J Indones 2010; 19:26-31)</strong<</em<</p<<p<<strong<Keywords:</strong< <em<spot specimen, special glass slide, fetal bovine serum</em<</p<
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title_short	Cytology technique: development of a simple spot method for cultured cell suspension
url	https://doi.org/10.13181/mji.v19i1.378 https://doaj.org/article/b642a5dc274843f8a538b0ce59d29a49 http://mji.ui.ac.id/journal/index.php/mji/article/view/378 https://doaj.org/toc/0853-1773 https://doaj.org/toc/2252-8083
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up_date	2024-07-03T22:05:11.638Z
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