In vitro regeneration and morphogenesis of somatic embryos of cassava
In this study, the somatic embryogenesis and regeneration of Cigana Preta plants from shoot apices and immature leaves taken from plants cultivated in vitro were examined. To embryo induction the explants were cultivated in Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic...
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| Autor*in: |
Ádila Melo Vidal [verfasserIn] Maria Angélica Pereira de Carvalho Costa [verfasserIn] Antônio da Silva Souza [verfasserIn] Weliton Antonio Bastos de Almeida [verfasserIn] Fernanda Vidigal Duarte Souza [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch ; Spanisch ; Portugiesisch |
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In: Revista Ciência Agronômica - Universidade Federal do Ceará, 2012 |
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| Links: |
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| DOI / URN: |
10.1590/S1806-66902014000300017 |
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| Katalog-ID: |
DOAJ034297588 |
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| 520 | |a In this study, the somatic embryogenesis and regeneration of Cigana Preta plants from shoot apices and immature leaves taken from plants cultivated in vitro were examined. To embryo induction the explants were cultivated in Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 4-amino-3,5,6-trichloropicolinic acid (Picloram) at concentrations of 8.0 and 12 mg L-1. To development of embryos two media cultures with different concentration of BAP (D1 or D2) were evaluated. Embryos in the cotyledonary stage were incubated in germination medium containing MS salts and vitamins, 2.0 µM copper sulfate, 2.4 g L-1 of Phytagel® and 1.77 µM BAP. The highest frequency of calluses and the greatest number of embryos per explant were obtained using the auxin picloram at a concentration of 8.0 mg L-1. The plants regenerated in the picloram treatment exhibited normal development and were transferred to a multiplication medium after a minimum of four weeks. The histological sections of the malformed embryos from foliar explants cultured in the presence of 2,4-D demonstrated that the origin of the cotyledonary structures was independent of the formation of the shoot apical meristem, which was not formed in the embryos, and the majority of the embryos were classified as cornet-shaped. This study demonstrates that in cassava, the use of different auxins provides different conditions for the formation of somatic embryos, and the low rate of conversion into plants results from abnormalities in the embryos. | ||
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10.1590/S1806-66902014000300017 doi (DE-627)DOAJ034297588 (DE-599)DOAJ3485c1cd5f9c4919943595025556e9c6 DE-627 ger DE-627 rakwb eng spa por S1-972 Ádila Melo Vidal verfasserin aut In vitro regeneration and morphogenesis of somatic embryos of cassava Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, the somatic embryogenesis and regeneration of Cigana Preta plants from shoot apices and immature leaves taken from plants cultivated in vitro were examined. To embryo induction the explants were cultivated in Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 4-amino-3,5,6-trichloropicolinic acid (Picloram) at concentrations of 8.0 and 12 mg L-1. To development of embryos two media cultures with different concentration of BAP (D1 or D2) were evaluated. Embryos in the cotyledonary stage were incubated in germination medium containing MS salts and vitamins, 2.0 µM copper sulfate, 2.4 g L-1 of Phytagel® and 1.77 µM BAP. The highest frequency of calluses and the greatest number of embryos per explant were obtained using the auxin picloram at a concentration of 8.0 mg L-1. The plants regenerated in the picloram treatment exhibited normal development and were transferred to a multiplication medium after a minimum of four weeks. The histological sections of the malformed embryos from foliar explants cultured in the presence of 2,4-D demonstrated that the origin of the cotyledonary structures was independent of the formation of the shoot apical meristem, which was not formed in the embryos, and the majority of the embryos were classified as cornet-shaped. This study demonstrates that in cassava, the use of different auxins provides different conditions for the formation of somatic embryos, and the low rate of conversion into plants results from abnormalities in the embryos. Cassava Somatic embryogenesis Regulators-plants Agriculture (General) Maria Angélica Pereira de Carvalho Costa verfasserin aut Antônio da Silva Souza verfasserin aut Weliton Antonio Bastos de Almeida verfasserin aut Fernanda Vidigal Duarte Souza verfasserin aut In Revista Ciência Agronômica Universidade Federal do Ceará, 2012 (DE-627)634762370 (DE-600)2572193-8 18066690 nnns https://doi.org/10.1590/S1806-66902014000300017 kostenfrei https://doaj.org/article/3485c1cd5f9c4919943595025556e9c6 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000300017&lng=en&tlng=en kostenfrei https://doaj.org/toc/1806-6690 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA AR |
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10.1590/S1806-66902014000300017 doi (DE-627)DOAJ034297588 (DE-599)DOAJ3485c1cd5f9c4919943595025556e9c6 DE-627 ger DE-627 rakwb eng spa por S1-972 Ádila Melo Vidal verfasserin aut In vitro regeneration and morphogenesis of somatic embryos of cassava Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, the somatic embryogenesis and regeneration of Cigana Preta plants from shoot apices and immature leaves taken from plants cultivated in vitro were examined. To embryo induction the explants were cultivated in Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 4-amino-3,5,6-trichloropicolinic acid (Picloram) at concentrations of 8.0 and 12 mg L-1. To development of embryos two media cultures with different concentration of BAP (D1 or D2) were evaluated. Embryos in the cotyledonary stage were incubated in germination medium containing MS salts and vitamins, 2.0 µM copper sulfate, 2.4 g L-1 of Phytagel® and 1.77 µM BAP. The highest frequency of calluses and the greatest number of embryos per explant were obtained using the auxin picloram at a concentration of 8.0 mg L-1. The plants regenerated in the picloram treatment exhibited normal development and were transferred to a multiplication medium after a minimum of four weeks. The histological sections of the malformed embryos from foliar explants cultured in the presence of 2,4-D demonstrated that the origin of the cotyledonary structures was independent of the formation of the shoot apical meristem, which was not formed in the embryos, and the majority of the embryos were classified as cornet-shaped. This study demonstrates that in cassava, the use of different auxins provides different conditions for the formation of somatic embryos, and the low rate of conversion into plants results from abnormalities in the embryos. Cassava Somatic embryogenesis Regulators-plants Agriculture (General) Maria Angélica Pereira de Carvalho Costa verfasserin aut Antônio da Silva Souza verfasserin aut Weliton Antonio Bastos de Almeida verfasserin aut Fernanda Vidigal Duarte Souza verfasserin aut In Revista Ciência Agronômica Universidade Federal do Ceará, 2012 (DE-627)634762370 (DE-600)2572193-8 18066690 nnns https://doi.org/10.1590/S1806-66902014000300017 kostenfrei https://doaj.org/article/3485c1cd5f9c4919943595025556e9c6 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000300017&lng=en&tlng=en kostenfrei https://doaj.org/toc/1806-6690 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA AR |
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In vitro regeneration and morphogenesis of somatic embryos of cassava |
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In vitro regeneration and morphogenesis of somatic embryos of cassava |
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Ádila Melo Vidal |
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Revista Ciência Agronômica |
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Ádila Melo Vidal Maria Angélica Pereira de Carvalho Costa Antônio da Silva Souza Weliton Antonio Bastos de Almeida Fernanda Vidigal Duarte Souza |
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Ádila Melo Vidal |
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10.1590/S1806-66902014000300017 |
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in vitro regeneration and morphogenesis of somatic embryos of cassava |
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In vitro regeneration and morphogenesis of somatic embryos of cassava |
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In this study, the somatic embryogenesis and regeneration of Cigana Preta plants from shoot apices and immature leaves taken from plants cultivated in vitro were examined. To embryo induction the explants were cultivated in Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 4-amino-3,5,6-trichloropicolinic acid (Picloram) at concentrations of 8.0 and 12 mg L-1. To development of embryos two media cultures with different concentration of BAP (D1 or D2) were evaluated. Embryos in the cotyledonary stage were incubated in germination medium containing MS salts and vitamins, 2.0 µM copper sulfate, 2.4 g L-1 of Phytagel® and 1.77 µM BAP. The highest frequency of calluses and the greatest number of embryos per explant were obtained using the auxin picloram at a concentration of 8.0 mg L-1. The plants regenerated in the picloram treatment exhibited normal development and were transferred to a multiplication medium after a minimum of four weeks. The histological sections of the malformed embryos from foliar explants cultured in the presence of 2,4-D demonstrated that the origin of the cotyledonary structures was independent of the formation of the shoot apical meristem, which was not formed in the embryos, and the majority of the embryos were classified as cornet-shaped. This study demonstrates that in cassava, the use of different auxins provides different conditions for the formation of somatic embryos, and the low rate of conversion into plants results from abnormalities in the embryos. |
| abstractGer |
In this study, the somatic embryogenesis and regeneration of Cigana Preta plants from shoot apices and immature leaves taken from plants cultivated in vitro were examined. To embryo induction the explants were cultivated in Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 4-amino-3,5,6-trichloropicolinic acid (Picloram) at concentrations of 8.0 and 12 mg L-1. To development of embryos two media cultures with different concentration of BAP (D1 or D2) were evaluated. Embryos in the cotyledonary stage were incubated in germination medium containing MS salts and vitamins, 2.0 µM copper sulfate, 2.4 g L-1 of Phytagel® and 1.77 µM BAP. The highest frequency of calluses and the greatest number of embryos per explant were obtained using the auxin picloram at a concentration of 8.0 mg L-1. The plants regenerated in the picloram treatment exhibited normal development and were transferred to a multiplication medium after a minimum of four weeks. The histological sections of the malformed embryos from foliar explants cultured in the presence of 2,4-D demonstrated that the origin of the cotyledonary structures was independent of the formation of the shoot apical meristem, which was not formed in the embryos, and the majority of the embryos were classified as cornet-shaped. This study demonstrates that in cassava, the use of different auxins provides different conditions for the formation of somatic embryos, and the low rate of conversion into plants results from abnormalities in the embryos. |
| abstract_unstemmed |
In this study, the somatic embryogenesis and regeneration of Cigana Preta plants from shoot apices and immature leaves taken from plants cultivated in vitro were examined. To embryo induction the explants were cultivated in Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 4-amino-3,5,6-trichloropicolinic acid (Picloram) at concentrations of 8.0 and 12 mg L-1. To development of embryos two media cultures with different concentration of BAP (D1 or D2) were evaluated. Embryos in the cotyledonary stage were incubated in germination medium containing MS salts and vitamins, 2.0 µM copper sulfate, 2.4 g L-1 of Phytagel® and 1.77 µM BAP. The highest frequency of calluses and the greatest number of embryos per explant were obtained using the auxin picloram at a concentration of 8.0 mg L-1. The plants regenerated in the picloram treatment exhibited normal development and were transferred to a multiplication medium after a minimum of four weeks. The histological sections of the malformed embryos from foliar explants cultured in the presence of 2,4-D demonstrated that the origin of the cotyledonary structures was independent of the formation of the shoot apical meristem, which was not formed in the embryos, and the majority of the embryos were classified as cornet-shaped. This study demonstrates that in cassava, the use of different auxins provides different conditions for the formation of somatic embryos, and the low rate of conversion into plants results from abnormalities in the embryos. |
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In vitro regeneration and morphogenesis of somatic embryos of cassava |
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https://doi.org/10.1590/S1806-66902014000300017 https://doaj.org/article/3485c1cd5f9c4919943595025556e9c6 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000300017&lng=en&tlng=en https://doaj.org/toc/1806-6690 |
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