Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of <i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst, a Multipurpose Woody Plant

<i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown <i<C</i<<i<. aconitifolius</i< plants was established for the first time. The sterilization effect, axillary bud initiation, and proliferation efficiency of stem segments were evaluated. The results showed that the sterilization time of 0.1% mercuric chloride, the concentration of Plant Preservative Mixture (PPM), the pretreatment method, and the sampling season had significant effects on the sterilization of stem segments (<i<p</i< < 0.05). The type of medium and plant growth regulators (PGRs) affected the initiation of axillary buds, and the proliferation efficiency was significantly affected by PGRs. The results showed that the best sterilization method for stem segment explants was as follows: a pretreatment by rinsing with running water for 120 min, soaking in 75% ethanol for 50 s, soaking in 0.1% mercuric chloride for 10 min, and medium supplemented with 3 mL/L PPM. When inoculated on the medium in spring, the contamination rate was as low as 25.56%. The optimal initiation medium for axillary buds in stem segments was half-strength Murashige and Skoog (1/2 MS) medium supplemented with 0.5 mg/L 6-benzyladenine (6-BA). The induction rate was as high as 93.33%, and the mean length of axillary buds was 2.47 cm. The optimal proliferation medium was 1/2 MS medium supplemented with 4.0 mg/L 6-BA and 0.2 mg/L indole-3-butyric acid (IBA). The induction rate was up to 80.00%, the total proliferation coefficient was 4.56, and the net proliferation coefficient was 5.69. The 1/2 MS medium supplemented with 0.1 mg/L 6-BA and 1.5 mg/L indole-3-acetic acid (IAA) was most conducive to the elongation of the adventitious shoot, and the adventitious shoot of approximately 1 cm reached 1.93 cm after culturing for 14 days. The best medium for adventitious shoot rooting was 1/2 MS medium supplemented with 0.1 mg/L α-naphthalene acetic acid (NAA), the highest rooting rate was 82.00%, and the survival rate of transplanting was over 90%. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Min Gu [verfasserIn] Youli Li [verfasserIn] Huier Jiang [verfasserIn] Shihu Zhang [verfasserIn] Qingmin Que [verfasserIn] Xiaoyang Chen [verfasserIn] Wei Zhou [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	aseptic system plant growth regulators tissue culture

Übergeordnetes Werk:	In: Plants - MDPI AG, 2013, 11(2022), 15, p 1937
Übergeordnetes Werk:	volume:11 ; year:2022 ; number:15, p 1937

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/plants11151937

Katalog-ID:	DOAJ034542213

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520			\|a <i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown <i<C</i<<i<. aconitifolius</i< plants was established for the first time. The sterilization effect, axillary bud initiation, and proliferation efficiency of stem segments were evaluated. The results showed that the sterilization time of 0.1% mercuric chloride, the concentration of Plant Preservative Mixture (PPM), the pretreatment method, and the sampling season had significant effects on the sterilization of stem segments (<i<p</i< < 0.05). The type of medium and plant growth regulators (PGRs) affected the initiation of axillary buds, and the proliferation efficiency was significantly affected by PGRs. The results showed that the best sterilization method for stem segment explants was as follows: a pretreatment by rinsing with running water for 120 min, soaking in 75% ethanol for 50 s, soaking in 0.1% mercuric chloride for 10 min, and medium supplemented with 3 mL/L PPM. When inoculated on the medium in spring, the contamination rate was as low as 25.56%. The optimal initiation medium for axillary buds in stem segments was half-strength Murashige and Skoog (1/2 MS) medium supplemented with 0.5 mg/L 6-benzyladenine (6-BA). The induction rate was as high as 93.33%, and the mean length of axillary buds was 2.47 cm. The optimal proliferation medium was 1/2 MS medium supplemented with 4.0 mg/L 6-BA and 0.2 mg/L indole-3-butyric acid (IBA). The induction rate was up to 80.00%, the total proliferation coefficient was 4.56, and the net proliferation coefficient was 5.69. The 1/2 MS medium supplemented with 0.1 mg/L 6-BA and 1.5 mg/L indole-3-acetic acid (IAA) was most conducive to the elongation of the adventitious shoot, and the adventitious shoot of approximately 1 cm reached 1.93 cm after culturing for 14 days. The best medium for adventitious shoot rooting was 1/2 MS medium supplemented with 0.1 mg/L α-naphthalene acetic acid (NAA), the highest rooting rate was 82.00%, and the survival rate of transplanting was over 90%.
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allfields	10.3390/plants11151937 doi (DE-627)DOAJ034542213 (DE-599)DOAJf40bb7cbf56c41dd8cd71bdb9b76e170 DE-627 ger DE-627 rakwb eng QK1-989 Min Gu verfasserin aut Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of <i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst, a Multipurpose Woody Plant 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown <i<C</i<<i<. aconitifolius</i< plants was established for the first time. The sterilization effect, axillary bud initiation, and proliferation efficiency of stem segments were evaluated. The results showed that the sterilization time of 0.1% mercuric chloride, the concentration of Plant Preservative Mixture (PPM), the pretreatment method, and the sampling season had significant effects on the sterilization of stem segments (<i<p</i< < 0.05). The type of medium and plant growth regulators (PGRs) affected the initiation of axillary buds, and the proliferation efficiency was significantly affected by PGRs. The results showed that the best sterilization method for stem segment explants was as follows: a pretreatment by rinsing with running water for 120 min, soaking in 75% ethanol for 50 s, soaking in 0.1% mercuric chloride for 10 min, and medium supplemented with 3 mL/L PPM. When inoculated on the medium in spring, the contamination rate was as low as 25.56%. The optimal initiation medium for axillary buds in stem segments was half-strength Murashige and Skoog (1/2 MS) medium supplemented with 0.5 mg/L 6-benzyladenine (6-BA). The induction rate was as high as 93.33%, and the mean length of axillary buds was 2.47 cm. The optimal proliferation medium was 1/2 MS medium supplemented with 4.0 mg/L 6-BA and 0.2 mg/L indole-3-butyric acid (IBA). The induction rate was up to 80.00%, the total proliferation coefficient was 4.56, and the net proliferation coefficient was 5.69. The 1/2 MS medium supplemented with 0.1 mg/L 6-BA and 1.5 mg/L indole-3-acetic acid (IAA) was most conducive to the elongation of the adventitious shoot, and the adventitious shoot of approximately 1 cm reached 1.93 cm after culturing for 14 days. The best medium for adventitious shoot rooting was 1/2 MS medium supplemented with 0.1 mg/L α-naphthalene acetic acid (NAA), the highest rooting rate was 82.00%, and the survival rate of transplanting was over 90%. <i<Cnidoscolus aconitifolius</i< aseptic system plant growth regulators tissue culture Botany Youli Li verfasserin aut Huier Jiang verfasserin aut Shihu Zhang verfasserin aut Qingmin Que verfasserin aut Xiaoyang Chen verfasserin aut Wei Zhou verfasserin aut In Plants MDPI AG, 2013 11(2022), 15, p 1937 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:11 year:2022 number:15, p 1937 https://doi.org/10.3390/plants11151937 kostenfrei https://doaj.org/article/f40bb7cbf56c41dd8cd71bdb9b76e170 kostenfrei https://www.mdpi.com/2223-7747/11/15/1937 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 15, p 1937
spelling	10.3390/plants11151937 doi (DE-627)DOAJ034542213 (DE-599)DOAJf40bb7cbf56c41dd8cd71bdb9b76e170 DE-627 ger DE-627 rakwb eng QK1-989 Min Gu verfasserin aut Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of <i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst, a Multipurpose Woody Plant 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown <i<C</i<<i<. aconitifolius</i< plants was established for the first time. The sterilization effect, axillary bud initiation, and proliferation efficiency of stem segments were evaluated. The results showed that the sterilization time of 0.1% mercuric chloride, the concentration of Plant Preservative Mixture (PPM), the pretreatment method, and the sampling season had significant effects on the sterilization of stem segments (<i<p</i< < 0.05). The type of medium and plant growth regulators (PGRs) affected the initiation of axillary buds, and the proliferation efficiency was significantly affected by PGRs. The results showed that the best sterilization method for stem segment explants was as follows: a pretreatment by rinsing with running water for 120 min, soaking in 75% ethanol for 50 s, soaking in 0.1% mercuric chloride for 10 min, and medium supplemented with 3 mL/L PPM. When inoculated on the medium in spring, the contamination rate was as low as 25.56%. The optimal initiation medium for axillary buds in stem segments was half-strength Murashige and Skoog (1/2 MS) medium supplemented with 0.5 mg/L 6-benzyladenine (6-BA). The induction rate was as high as 93.33%, and the mean length of axillary buds was 2.47 cm. The optimal proliferation medium was 1/2 MS medium supplemented with 4.0 mg/L 6-BA and 0.2 mg/L indole-3-butyric acid (IBA). The induction rate was up to 80.00%, the total proliferation coefficient was 4.56, and the net proliferation coefficient was 5.69. The 1/2 MS medium supplemented with 0.1 mg/L 6-BA and 1.5 mg/L indole-3-acetic acid (IAA) was most conducive to the elongation of the adventitious shoot, and the adventitious shoot of approximately 1 cm reached 1.93 cm after culturing for 14 days. The best medium for adventitious shoot rooting was 1/2 MS medium supplemented with 0.1 mg/L α-naphthalene acetic acid (NAA), the highest rooting rate was 82.00%, and the survival rate of transplanting was over 90%. <i<Cnidoscolus aconitifolius</i< aseptic system plant growth regulators tissue culture Botany Youli Li verfasserin aut Huier Jiang verfasserin aut Shihu Zhang verfasserin aut Qingmin Que verfasserin aut Xiaoyang Chen verfasserin aut Wei Zhou verfasserin aut In Plants MDPI AG, 2013 11(2022), 15, p 1937 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:11 year:2022 number:15, p 1937 https://doi.org/10.3390/plants11151937 kostenfrei https://doaj.org/article/f40bb7cbf56c41dd8cd71bdb9b76e170 kostenfrei https://www.mdpi.com/2223-7747/11/15/1937 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 15, p 1937
allfields_unstemmed	10.3390/plants11151937 doi (DE-627)DOAJ034542213 (DE-599)DOAJf40bb7cbf56c41dd8cd71bdb9b76e170 DE-627 ger DE-627 rakwb eng QK1-989 Min Gu verfasserin aut Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of <i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst, a Multipurpose Woody Plant 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown <i<C</i<<i<. aconitifolius</i< plants was established for the first time. The sterilization effect, axillary bud initiation, and proliferation efficiency of stem segments were evaluated. The results showed that the sterilization time of 0.1% mercuric chloride, the concentration of Plant Preservative Mixture (PPM), the pretreatment method, and the sampling season had significant effects on the sterilization of stem segments (<i<p</i< < 0.05). The type of medium and plant growth regulators (PGRs) affected the initiation of axillary buds, and the proliferation efficiency was significantly affected by PGRs. The results showed that the best sterilization method for stem segment explants was as follows: a pretreatment by rinsing with running water for 120 min, soaking in 75% ethanol for 50 s, soaking in 0.1% mercuric chloride for 10 min, and medium supplemented with 3 mL/L PPM. When inoculated on the medium in spring, the contamination rate was as low as 25.56%. The optimal initiation medium for axillary buds in stem segments was half-strength Murashige and Skoog (1/2 MS) medium supplemented with 0.5 mg/L 6-benzyladenine (6-BA). The induction rate was as high as 93.33%, and the mean length of axillary buds was 2.47 cm. The optimal proliferation medium was 1/2 MS medium supplemented with 4.0 mg/L 6-BA and 0.2 mg/L indole-3-butyric acid (IBA). The induction rate was up to 80.00%, the total proliferation coefficient was 4.56, and the net proliferation coefficient was 5.69. The 1/2 MS medium supplemented with 0.1 mg/L 6-BA and 1.5 mg/L indole-3-acetic acid (IAA) was most conducive to the elongation of the adventitious shoot, and the adventitious shoot of approximately 1 cm reached 1.93 cm after culturing for 14 days. The best medium for adventitious shoot rooting was 1/2 MS medium supplemented with 0.1 mg/L α-naphthalene acetic acid (NAA), the highest rooting rate was 82.00%, and the survival rate of transplanting was over 90%. <i<Cnidoscolus aconitifolius</i< aseptic system plant growth regulators tissue culture Botany Youli Li verfasserin aut Huier Jiang verfasserin aut Shihu Zhang verfasserin aut Qingmin Que verfasserin aut Xiaoyang Chen verfasserin aut Wei Zhou verfasserin aut In Plants MDPI AG, 2013 11(2022), 15, p 1937 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:11 year:2022 number:15, p 1937 https://doi.org/10.3390/plants11151937 kostenfrei https://doaj.org/article/f40bb7cbf56c41dd8cd71bdb9b76e170 kostenfrei https://www.mdpi.com/2223-7747/11/15/1937 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 15, p 1937
allfieldsGer	10.3390/plants11151937 doi (DE-627)DOAJ034542213 (DE-599)DOAJf40bb7cbf56c41dd8cd71bdb9b76e170 DE-627 ger DE-627 rakwb eng QK1-989 Min Gu verfasserin aut Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of <i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst, a Multipurpose Woody Plant 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown <i<C</i<<i<. aconitifolius</i< plants was established for the first time. The sterilization effect, axillary bud initiation, and proliferation efficiency of stem segments were evaluated. The results showed that the sterilization time of 0.1% mercuric chloride, the concentration of Plant Preservative Mixture (PPM), the pretreatment method, and the sampling season had significant effects on the sterilization of stem segments (<i<p</i< < 0.05). The type of medium and plant growth regulators (PGRs) affected the initiation of axillary buds, and the proliferation efficiency was significantly affected by PGRs. The results showed that the best sterilization method for stem segment explants was as follows: a pretreatment by rinsing with running water for 120 min, soaking in 75% ethanol for 50 s, soaking in 0.1% mercuric chloride for 10 min, and medium supplemented with 3 mL/L PPM. When inoculated on the medium in spring, the contamination rate was as low as 25.56%. The optimal initiation medium for axillary buds in stem segments was half-strength Murashige and Skoog (1/2 MS) medium supplemented with 0.5 mg/L 6-benzyladenine (6-BA). The induction rate was as high as 93.33%, and the mean length of axillary buds was 2.47 cm. The optimal proliferation medium was 1/2 MS medium supplemented with 4.0 mg/L 6-BA and 0.2 mg/L indole-3-butyric acid (IBA). The induction rate was up to 80.00%, the total proliferation coefficient was 4.56, and the net proliferation coefficient was 5.69. The 1/2 MS medium supplemented with 0.1 mg/L 6-BA and 1.5 mg/L indole-3-acetic acid (IAA) was most conducive to the elongation of the adventitious shoot, and the adventitious shoot of approximately 1 cm reached 1.93 cm after culturing for 14 days. The best medium for adventitious shoot rooting was 1/2 MS medium supplemented with 0.1 mg/L α-naphthalene acetic acid (NAA), the highest rooting rate was 82.00%, and the survival rate of transplanting was over 90%. <i<Cnidoscolus aconitifolius</i< aseptic system plant growth regulators tissue culture Botany Youli Li verfasserin aut Huier Jiang verfasserin aut Shihu Zhang verfasserin aut Qingmin Que verfasserin aut Xiaoyang Chen verfasserin aut Wei Zhou verfasserin aut In Plants MDPI AG, 2013 11(2022), 15, p 1937 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:11 year:2022 number:15, p 1937 https://doi.org/10.3390/plants11151937 kostenfrei https://doaj.org/article/f40bb7cbf56c41dd8cd71bdb9b76e170 kostenfrei https://www.mdpi.com/2223-7747/11/15/1937 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 15, p 1937
allfieldsSound	10.3390/plants11151937 doi (DE-627)DOAJ034542213 (DE-599)DOAJf40bb7cbf56c41dd8cd71bdb9b76e170 DE-627 ger DE-627 rakwb eng QK1-989 Min Gu verfasserin aut Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of <i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst, a Multipurpose Woody Plant 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown <i<C</i<<i<. aconitifolius</i< plants was established for the first time. The sterilization effect, axillary bud initiation, and proliferation efficiency of stem segments were evaluated. The results showed that the sterilization time of 0.1% mercuric chloride, the concentration of Plant Preservative Mixture (PPM), the pretreatment method, and the sampling season had significant effects on the sterilization of stem segments (<i<p</i< < 0.05). The type of medium and plant growth regulators (PGRs) affected the initiation of axillary buds, and the proliferation efficiency was significantly affected by PGRs. The results showed that the best sterilization method for stem segment explants was as follows: a pretreatment by rinsing with running water for 120 min, soaking in 75% ethanol for 50 s, soaking in 0.1% mercuric chloride for 10 min, and medium supplemented with 3 mL/L PPM. When inoculated on the medium in spring, the contamination rate was as low as 25.56%. The optimal initiation medium for axillary buds in stem segments was half-strength Murashige and Skoog (1/2 MS) medium supplemented with 0.5 mg/L 6-benzyladenine (6-BA). The induction rate was as high as 93.33%, and the mean length of axillary buds was 2.47 cm. The optimal proliferation medium was 1/2 MS medium supplemented with 4.0 mg/L 6-BA and 0.2 mg/L indole-3-butyric acid (IBA). The induction rate was up to 80.00%, the total proliferation coefficient was 4.56, and the net proliferation coefficient was 5.69. The 1/2 MS medium supplemented with 0.1 mg/L 6-BA and 1.5 mg/L indole-3-acetic acid (IAA) was most conducive to the elongation of the adventitious shoot, and the adventitious shoot of approximately 1 cm reached 1.93 cm after culturing for 14 days. The best medium for adventitious shoot rooting was 1/2 MS medium supplemented with 0.1 mg/L α-naphthalene acetic acid (NAA), the highest rooting rate was 82.00%, and the survival rate of transplanting was over 90%. <i<Cnidoscolus aconitifolius</i< aseptic system plant growth regulators tissue culture Botany Youli Li verfasserin aut Huier Jiang verfasserin aut Shihu Zhang verfasserin aut Qingmin Que verfasserin aut Xiaoyang Chen verfasserin aut Wei Zhou verfasserin aut In Plants MDPI AG, 2013 11(2022), 15, p 1937 (DE-627)737288345 (DE-600)2704341-1 22237747 nnns volume:11 year:2022 number:15, p 1937 https://doi.org/10.3390/plants11151937 kostenfrei https://doaj.org/article/f40bb7cbf56c41dd8cd71bdb9b76e170 kostenfrei https://www.mdpi.com/2223-7747/11/15/1937 kostenfrei https://doaj.org/toc/2223-7747 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 15, p 1937
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authorswithroles_txt_mv	Min Gu @@aut@@ Youli Li @@aut@@ Huier Jiang @@aut@@ Shihu Zhang @@aut@@ Qingmin Que @@aut@@ Xiaoyang Chen @@aut@@ Wei Zhou @@aut@@
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topic_title	QK1-989 Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of <i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst, a Multipurpose Woody Plant <i<Cnidoscolus aconitifolius</i< aseptic system plant growth regulators tissue culture
topic	misc QK1-989 misc <i<Cnidoscolus aconitifolius</i< misc aseptic system misc plant growth regulators misc tissue culture misc Botany
topic_unstemmed	misc QK1-989 misc <i<Cnidoscolus aconitifolius</i< misc aseptic system misc plant growth regulators misc tissue culture misc Botany
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title	Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of <i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst, a Multipurpose Woody Plant
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title_full	Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of <i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst, a Multipurpose Woody Plant
author_sort	Min Gu
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author_browse	Min Gu Youli Li Huier Jiang Shihu Zhang Qingmin Que Xiaoyang Chen Wei Zhou
container_volume	11
class	QK1-989
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doi_str_mv	10.3390/plants11151937
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title_sort	efficient in vitro sterilization and propagation from stem segment explants of <i<cnidoscolus aconitifolius</i< (mill.) i.m. johnst, a multipurpose woody plant
callnumber	QK1-989
title_auth	Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of <i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst, a Multipurpose Woody Plant
abstract	<i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown <i<C</i<<i<. aconitifolius</i< plants was established for the first time. The sterilization effect, axillary bud initiation, and proliferation efficiency of stem segments were evaluated. The results showed that the sterilization time of 0.1% mercuric chloride, the concentration of Plant Preservative Mixture (PPM), the pretreatment method, and the sampling season had significant effects on the sterilization of stem segments (<i<p</i< < 0.05). The type of medium and plant growth regulators (PGRs) affected the initiation of axillary buds, and the proliferation efficiency was significantly affected by PGRs. The results showed that the best sterilization method for stem segment explants was as follows: a pretreatment by rinsing with running water for 120 min, soaking in 75% ethanol for 50 s, soaking in 0.1% mercuric chloride for 10 min, and medium supplemented with 3 mL/L PPM. When inoculated on the medium in spring, the contamination rate was as low as 25.56%. The optimal initiation medium for axillary buds in stem segments was half-strength Murashige and Skoog (1/2 MS) medium supplemented with 0.5 mg/L 6-benzyladenine (6-BA). The induction rate was as high as 93.33%, and the mean length of axillary buds was 2.47 cm. The optimal proliferation medium was 1/2 MS medium supplemented with 4.0 mg/L 6-BA and 0.2 mg/L indole-3-butyric acid (IBA). The induction rate was up to 80.00%, the total proliferation coefficient was 4.56, and the net proliferation coefficient was 5.69. The 1/2 MS medium supplemented with 0.1 mg/L 6-BA and 1.5 mg/L indole-3-acetic acid (IAA) was most conducive to the elongation of the adventitious shoot, and the adventitious shoot of approximately 1 cm reached 1.93 cm after culturing for 14 days. The best medium for adventitious shoot rooting was 1/2 MS medium supplemented with 0.1 mg/L α-naphthalene acetic acid (NAA), the highest rooting rate was 82.00%, and the survival rate of transplanting was over 90%.
abstractGer	<i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown <i<C</i<<i<. aconitifolius</i< plants was established for the first time. The sterilization effect, axillary bud initiation, and proliferation efficiency of stem segments were evaluated. The results showed that the sterilization time of 0.1% mercuric chloride, the concentration of Plant Preservative Mixture (PPM), the pretreatment method, and the sampling season had significant effects on the sterilization of stem segments (<i<p</i< < 0.05). The type of medium and plant growth regulators (PGRs) affected the initiation of axillary buds, and the proliferation efficiency was significantly affected by PGRs. The results showed that the best sterilization method for stem segment explants was as follows: a pretreatment by rinsing with running water for 120 min, soaking in 75% ethanol for 50 s, soaking in 0.1% mercuric chloride for 10 min, and medium supplemented with 3 mL/L PPM. When inoculated on the medium in spring, the contamination rate was as low as 25.56%. The optimal initiation medium for axillary buds in stem segments was half-strength Murashige and Skoog (1/2 MS) medium supplemented with 0.5 mg/L 6-benzyladenine (6-BA). The induction rate was as high as 93.33%, and the mean length of axillary buds was 2.47 cm. The optimal proliferation medium was 1/2 MS medium supplemented with 4.0 mg/L 6-BA and 0.2 mg/L indole-3-butyric acid (IBA). The induction rate was up to 80.00%, the total proliferation coefficient was 4.56, and the net proliferation coefficient was 5.69. The 1/2 MS medium supplemented with 0.1 mg/L 6-BA and 1.5 mg/L indole-3-acetic acid (IAA) was most conducive to the elongation of the adventitious shoot, and the adventitious shoot of approximately 1 cm reached 1.93 cm after culturing for 14 days. The best medium for adventitious shoot rooting was 1/2 MS medium supplemented with 0.1 mg/L α-naphthalene acetic acid (NAA), the highest rooting rate was 82.00%, and the survival rate of transplanting was over 90%.
abstract_unstemmed	<i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown <i<C</i<<i<. aconitifolius</i< plants was established for the first time. The sterilization effect, axillary bud initiation, and proliferation efficiency of stem segments were evaluated. The results showed that the sterilization time of 0.1% mercuric chloride, the concentration of Plant Preservative Mixture (PPM), the pretreatment method, and the sampling season had significant effects on the sterilization of stem segments (<i<p</i< < 0.05). The type of medium and plant growth regulators (PGRs) affected the initiation of axillary buds, and the proliferation efficiency was significantly affected by PGRs. The results showed that the best sterilization method for stem segment explants was as follows: a pretreatment by rinsing with running water for 120 min, soaking in 75% ethanol for 50 s, soaking in 0.1% mercuric chloride for 10 min, and medium supplemented with 3 mL/L PPM. When inoculated on the medium in spring, the contamination rate was as low as 25.56%. The optimal initiation medium for axillary buds in stem segments was half-strength Murashige and Skoog (1/2 MS) medium supplemented with 0.5 mg/L 6-benzyladenine (6-BA). The induction rate was as high as 93.33%, and the mean length of axillary buds was 2.47 cm. The optimal proliferation medium was 1/2 MS medium supplemented with 4.0 mg/L 6-BA and 0.2 mg/L indole-3-butyric acid (IBA). The induction rate was up to 80.00%, the total proliferation coefficient was 4.56, and the net proliferation coefficient was 5.69. The 1/2 MS medium supplemented with 0.1 mg/L 6-BA and 1.5 mg/L indole-3-acetic acid (IAA) was most conducive to the elongation of the adventitious shoot, and the adventitious shoot of approximately 1 cm reached 1.93 cm after culturing for 14 days. The best medium for adventitious shoot rooting was 1/2 MS medium supplemented with 0.1 mg/L α-naphthalene acetic acid (NAA), the highest rooting rate was 82.00%, and the survival rate of transplanting was over 90%.
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container_issue	15, p 1937
title_short	Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of <i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst, a Multipurpose Woody Plant
url	https://doi.org/10.3390/plants11151937 https://doaj.org/article/f40bb7cbf56c41dd8cd71bdb9b76e170 https://www.mdpi.com/2223-7747/11/15/1937 https://doaj.org/toc/2223-7747
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Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of <i<Cnidoscolus aconitifolius</i< (Mill.) I.M. Johnst, a Multipurpose Woody Plant

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