Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms

Background: Recent advances demonstrate the role of chromatin regulators, including histone variants and histone chaperones, in cancer initiation and progression. Methods: Histone H3K4me3, histone variant centromere protein (CENP-A) and histone chaperones Holliday junction recognition protein (HJURP...
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Autor*in:	Georgia Levidou [verfasserIn] Konstantinos Palamaris [verfasserIn] Alexandros G. Sykaras [verfasserIn] Georgios Andreadakis [verfasserIn] Christos Masaoutis [verfasserIn] Irene Theochari [verfasserIn] Penelope Korkolopoulou [verfasserIn] Dimitra Rontogianni [verfasserIn] Stamatios Theocharis [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	thymic epithelial neoplasms thymomas histones CENP-A HJURP DAXX

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 23(2022), 15, p 8339
Übergeordnetes Werk:	volume:23 ; year:2022 ; number:15, p 8339

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms23158339

Katalog-ID:	DOAJ034547134

Internformat


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520			\|a Background: Recent advances demonstrate the role of chromatin regulators, including histone variants and histone chaperones, in cancer initiation and progression. Methods: Histone H3K4me3, histone variant centromere protein (CENP-A) and histone chaperones Holliday junction recognition protein (HJURP) as well as DAXX expression were examined immunohistochemically in 95 thymic epithelial tumor (TET) specimens. Our results were compared with the expression profile of DAXX, HJURP and CENP-A in gene expression profiling interactive analysis (GEPIA2). Results: The lymphocyte-poor B3- and C-type TETs were more frequently DAXX negative (<i<p</i< = 0.043). B3 and C-Type TETs showed higher cytoplasmic and nuclear CENP-A (<i<p</i< = 0.007 and <i<p</i< = 0.002) and higher cytoplasmic HJURP H-score (<i<p</i< < 0.001). Higher nuclear CENP-A and cytoplasmic HJURP expression was associated with advanced Masaoka–Koga stage (<i<p</i< = 0.048 and <i<p</i< < 0.001). A positive correlation between HJURP and CENP-A was also observed. The presence of cytoplasmic CENP-A expression was correlated with a favorable overall survival (<i<p</i< = 0.03). CENP-A overexpression in survival analysis of TCGA TETs showed similar results. H3K4me3 expression was not associated with any clinicopathological parameters. Conclusions: Our results suggest a significant interaction between CENP-A and HJURP in TETs. Moreover, we confirmed the presence of a cytoplasmic CENP-A immunolocalization, suggesting also a possible favorable prognostic value of this specific immunostaining pattern.
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allfields	10.3390/ijms23158339 doi (DE-627)DOAJ034547134 (DE-599)DOAJbd6356ee7ab84267b42d9a16ddef88dd DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Georgia Levidou verfasserin aut Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Recent advances demonstrate the role of chromatin regulators, including histone variants and histone chaperones, in cancer initiation and progression. Methods: Histone H3K4me3, histone variant centromere protein (CENP-A) and histone chaperones Holliday junction recognition protein (HJURP) as well as DAXX expression were examined immunohistochemically in 95 thymic epithelial tumor (TET) specimens. Our results were compared with the expression profile of DAXX, HJURP and CENP-A in gene expression profiling interactive analysis (GEPIA2). Results: The lymphocyte-poor B3- and C-type TETs were more frequently DAXX negative (<i<p</i< = 0.043). B3 and C-Type TETs showed higher cytoplasmic and nuclear CENP-A (<i<p</i< = 0.007 and <i<p</i< = 0.002) and higher cytoplasmic HJURP H-score (<i<p</i< < 0.001). Higher nuclear CENP-A and cytoplasmic HJURP expression was associated with advanced Masaoka–Koga stage (<i<p</i< = 0.048 and <i<p</i< < 0.001). A positive correlation between HJURP and CENP-A was also observed. The presence of cytoplasmic CENP-A expression was correlated with a favorable overall survival (<i<p</i< = 0.03). CENP-A overexpression in survival analysis of TCGA TETs showed similar results. H3K4me3 expression was not associated with any clinicopathological parameters. Conclusions: Our results suggest a significant interaction between CENP-A and HJURP in TETs. Moreover, we confirmed the presence of a cytoplasmic CENP-A immunolocalization, suggesting also a possible favorable prognostic value of this specific immunostaining pattern. thymic epithelial neoplasms thymomas histones CENP-A HJURP DAXX Biology (General) Chemistry Konstantinos Palamaris verfasserin aut Alexandros G. Sykaras verfasserin aut Georgios Andreadakis verfasserin aut Christos Masaoutis verfasserin aut Irene Theochari verfasserin aut Penelope Korkolopoulou verfasserin aut Dimitra Rontogianni verfasserin aut Stamatios Theocharis verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 15, p 8339 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:15, p 8339 https://doi.org/10.3390/ijms23158339 kostenfrei https://doaj.org/article/bd6356ee7ab84267b42d9a16ddef88dd kostenfrei https://www.mdpi.com/1422-0067/23/15/8339 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 15, p 8339
spelling	10.3390/ijms23158339 doi (DE-627)DOAJ034547134 (DE-599)DOAJbd6356ee7ab84267b42d9a16ddef88dd DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Georgia Levidou verfasserin aut Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Recent advances demonstrate the role of chromatin regulators, including histone variants and histone chaperones, in cancer initiation and progression. Methods: Histone H3K4me3, histone variant centromere protein (CENP-A) and histone chaperones Holliday junction recognition protein (HJURP) as well as DAXX expression were examined immunohistochemically in 95 thymic epithelial tumor (TET) specimens. Our results were compared with the expression profile of DAXX, HJURP and CENP-A in gene expression profiling interactive analysis (GEPIA2). Results: The lymphocyte-poor B3- and C-type TETs were more frequently DAXX negative (<i<p</i< = 0.043). B3 and C-Type TETs showed higher cytoplasmic and nuclear CENP-A (<i<p</i< = 0.007 and <i<p</i< = 0.002) and higher cytoplasmic HJURP H-score (<i<p</i< < 0.001). Higher nuclear CENP-A and cytoplasmic HJURP expression was associated with advanced Masaoka–Koga stage (<i<p</i< = 0.048 and <i<p</i< < 0.001). A positive correlation between HJURP and CENP-A was also observed. The presence of cytoplasmic CENP-A expression was correlated with a favorable overall survival (<i<p</i< = 0.03). CENP-A overexpression in survival analysis of TCGA TETs showed similar results. H3K4me3 expression was not associated with any clinicopathological parameters. Conclusions: Our results suggest a significant interaction between CENP-A and HJURP in TETs. Moreover, we confirmed the presence of a cytoplasmic CENP-A immunolocalization, suggesting also a possible favorable prognostic value of this specific immunostaining pattern. thymic epithelial neoplasms thymomas histones CENP-A HJURP DAXX Biology (General) Chemistry Konstantinos Palamaris verfasserin aut Alexandros G. Sykaras verfasserin aut Georgios Andreadakis verfasserin aut Christos Masaoutis verfasserin aut Irene Theochari verfasserin aut Penelope Korkolopoulou verfasserin aut Dimitra Rontogianni verfasserin aut Stamatios Theocharis verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 15, p 8339 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:15, p 8339 https://doi.org/10.3390/ijms23158339 kostenfrei https://doaj.org/article/bd6356ee7ab84267b42d9a16ddef88dd kostenfrei https://www.mdpi.com/1422-0067/23/15/8339 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 15, p 8339
allfields_unstemmed	10.3390/ijms23158339 doi (DE-627)DOAJ034547134 (DE-599)DOAJbd6356ee7ab84267b42d9a16ddef88dd DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Georgia Levidou verfasserin aut Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Recent advances demonstrate the role of chromatin regulators, including histone variants and histone chaperones, in cancer initiation and progression. Methods: Histone H3K4me3, histone variant centromere protein (CENP-A) and histone chaperones Holliday junction recognition protein (HJURP) as well as DAXX expression were examined immunohistochemically in 95 thymic epithelial tumor (TET) specimens. Our results were compared with the expression profile of DAXX, HJURP and CENP-A in gene expression profiling interactive analysis (GEPIA2). Results: The lymphocyte-poor B3- and C-type TETs were more frequently DAXX negative (<i<p</i< = 0.043). B3 and C-Type TETs showed higher cytoplasmic and nuclear CENP-A (<i<p</i< = 0.007 and <i<p</i< = 0.002) and higher cytoplasmic HJURP H-score (<i<p</i< < 0.001). Higher nuclear CENP-A and cytoplasmic HJURP expression was associated with advanced Masaoka–Koga stage (<i<p</i< = 0.048 and <i<p</i< < 0.001). A positive correlation between HJURP and CENP-A was also observed. The presence of cytoplasmic CENP-A expression was correlated with a favorable overall survival (<i<p</i< = 0.03). CENP-A overexpression in survival analysis of TCGA TETs showed similar results. H3K4me3 expression was not associated with any clinicopathological parameters. Conclusions: Our results suggest a significant interaction between CENP-A and HJURP in TETs. Moreover, we confirmed the presence of a cytoplasmic CENP-A immunolocalization, suggesting also a possible favorable prognostic value of this specific immunostaining pattern. thymic epithelial neoplasms thymomas histones CENP-A HJURP DAXX Biology (General) Chemistry Konstantinos Palamaris verfasserin aut Alexandros G. Sykaras verfasserin aut Georgios Andreadakis verfasserin aut Christos Masaoutis verfasserin aut Irene Theochari verfasserin aut Penelope Korkolopoulou verfasserin aut Dimitra Rontogianni verfasserin aut Stamatios Theocharis verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 15, p 8339 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:15, p 8339 https://doi.org/10.3390/ijms23158339 kostenfrei https://doaj.org/article/bd6356ee7ab84267b42d9a16ddef88dd kostenfrei https://www.mdpi.com/1422-0067/23/15/8339 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 15, p 8339
allfieldsGer	10.3390/ijms23158339 doi (DE-627)DOAJ034547134 (DE-599)DOAJbd6356ee7ab84267b42d9a16ddef88dd DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Georgia Levidou verfasserin aut Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Recent advances demonstrate the role of chromatin regulators, including histone variants and histone chaperones, in cancer initiation and progression. Methods: Histone H3K4me3, histone variant centromere protein (CENP-A) and histone chaperones Holliday junction recognition protein (HJURP) as well as DAXX expression were examined immunohistochemically in 95 thymic epithelial tumor (TET) specimens. Our results were compared with the expression profile of DAXX, HJURP and CENP-A in gene expression profiling interactive analysis (GEPIA2). Results: The lymphocyte-poor B3- and C-type TETs were more frequently DAXX negative (<i<p</i< = 0.043). B3 and C-Type TETs showed higher cytoplasmic and nuclear CENP-A (<i<p</i< = 0.007 and <i<p</i< = 0.002) and higher cytoplasmic HJURP H-score (<i<p</i< < 0.001). Higher nuclear CENP-A and cytoplasmic HJURP expression was associated with advanced Masaoka–Koga stage (<i<p</i< = 0.048 and <i<p</i< < 0.001). A positive correlation between HJURP and CENP-A was also observed. The presence of cytoplasmic CENP-A expression was correlated with a favorable overall survival (<i<p</i< = 0.03). CENP-A overexpression in survival analysis of TCGA TETs showed similar results. H3K4me3 expression was not associated with any clinicopathological parameters. Conclusions: Our results suggest a significant interaction between CENP-A and HJURP in TETs. Moreover, we confirmed the presence of a cytoplasmic CENP-A immunolocalization, suggesting also a possible favorable prognostic value of this specific immunostaining pattern. thymic epithelial neoplasms thymomas histones CENP-A HJURP DAXX Biology (General) Chemistry Konstantinos Palamaris verfasserin aut Alexandros G. Sykaras verfasserin aut Georgios Andreadakis verfasserin aut Christos Masaoutis verfasserin aut Irene Theochari verfasserin aut Penelope Korkolopoulou verfasserin aut Dimitra Rontogianni verfasserin aut Stamatios Theocharis verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 15, p 8339 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:15, p 8339 https://doi.org/10.3390/ijms23158339 kostenfrei https://doaj.org/article/bd6356ee7ab84267b42d9a16ddef88dd kostenfrei https://www.mdpi.com/1422-0067/23/15/8339 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 15, p 8339
allfieldsSound	10.3390/ijms23158339 doi (DE-627)DOAJ034547134 (DE-599)DOAJbd6356ee7ab84267b42d9a16ddef88dd DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Georgia Levidou verfasserin aut Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Recent advances demonstrate the role of chromatin regulators, including histone variants and histone chaperones, in cancer initiation and progression. Methods: Histone H3K4me3, histone variant centromere protein (CENP-A) and histone chaperones Holliday junction recognition protein (HJURP) as well as DAXX expression were examined immunohistochemically in 95 thymic epithelial tumor (TET) specimens. Our results were compared with the expression profile of DAXX, HJURP and CENP-A in gene expression profiling interactive analysis (GEPIA2). Results: The lymphocyte-poor B3- and C-type TETs were more frequently DAXX negative (<i<p</i< = 0.043). B3 and C-Type TETs showed higher cytoplasmic and nuclear CENP-A (<i<p</i< = 0.007 and <i<p</i< = 0.002) and higher cytoplasmic HJURP H-score (<i<p</i< < 0.001). Higher nuclear CENP-A and cytoplasmic HJURP expression was associated with advanced Masaoka–Koga stage (<i<p</i< = 0.048 and <i<p</i< < 0.001). A positive correlation between HJURP and CENP-A was also observed. The presence of cytoplasmic CENP-A expression was correlated with a favorable overall survival (<i<p</i< = 0.03). CENP-A overexpression in survival analysis of TCGA TETs showed similar results. H3K4me3 expression was not associated with any clinicopathological parameters. Conclusions: Our results suggest a significant interaction between CENP-A and HJURP in TETs. Moreover, we confirmed the presence of a cytoplasmic CENP-A immunolocalization, suggesting also a possible favorable prognostic value of this specific immunostaining pattern. thymic epithelial neoplasms thymomas histones CENP-A HJURP DAXX Biology (General) Chemistry Konstantinos Palamaris verfasserin aut Alexandros G. Sykaras verfasserin aut Georgios Andreadakis verfasserin aut Christos Masaoutis verfasserin aut Irene Theochari verfasserin aut Penelope Korkolopoulou verfasserin aut Dimitra Rontogianni verfasserin aut Stamatios Theocharis verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 15, p 8339 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:15, p 8339 https://doi.org/10.3390/ijms23158339 kostenfrei https://doaj.org/article/bd6356ee7ab84267b42d9a16ddef88dd kostenfrei https://www.mdpi.com/1422-0067/23/15/8339 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 15, p 8339
language	English
source	In International Journal of Molecular Sciences 23(2022), 15, p 8339 volume:23 year:2022 number:15, p 8339
sourceStr	In International Journal of Molecular Sciences 23(2022), 15, p 8339 volume:23 year:2022 number:15, p 8339
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	thymic epithelial neoplasms thymomas histones CENP-A HJURP DAXX Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Georgia Levidou @@aut@@ Konstantinos Palamaris @@aut@@ Alexandros G. Sykaras @@aut@@ Georgios Andreadakis @@aut@@ Christos Masaoutis @@aut@@ Irene Theochari @@aut@@ Penelope Korkolopoulou @@aut@@ Dimitra Rontogianni @@aut@@ Stamatios Theocharis @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ034547134
language_de	englisch
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Methods: Histone H3K4me3, histone variant centromere protein (CENP-A) and histone chaperones Holliday junction recognition protein (HJURP) as well as DAXX expression were examined immunohistochemically in 95 thymic epithelial tumor (TET) specimens. Our results were compared with the expression profile of DAXX, HJURP and CENP-A in gene expression profiling interactive analysis (GEPIA2). Results: The lymphocyte-poor B3- and C-type TETs were more frequently DAXX negative (<i<p</i< = 0.043). B3 and C-Type TETs showed higher cytoplasmic and nuclear CENP-A (<i<p</i< = 0.007 and <i<p</i< = 0.002) and higher cytoplasmic HJURP H-score (<i<p</i< < 0.001). Higher nuclear CENP-A and cytoplasmic HJURP expression was associated with advanced Masaoka–Koga stage (<i<p</i< = 0.048 and <i<p</i< < 0.001). A positive correlation between HJURP and CENP-A was also observed. The presence of cytoplasmic CENP-A expression was correlated with a favorable overall survival (<i<p</i< = 0.03). CENP-A overexpression in survival analysis of TCGA TETs showed similar results. H3K4me3 expression was not associated with any clinicopathological parameters. Conclusions: Our results suggest a significant interaction between CENP-A and HJURP in TETs. 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callnumber-first	Q - Science
author	Georgia Levidou
spellingShingle	Georgia Levidou misc QH301-705.5 misc QD1-999 misc thymic epithelial neoplasms misc thymomas misc histones misc CENP-A misc HJURP misc DAXX misc Biology (General) misc Chemistry Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms
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topic_title	QH301-705.5 QD1-999 Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms thymic epithelial neoplasms thymomas histones CENP-A HJURP DAXX
topic	misc QH301-705.5 misc QD1-999 misc thymic epithelial neoplasms misc thymomas misc histones misc CENP-A misc HJURP misc DAXX misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc thymic epithelial neoplasms misc thymomas misc histones misc CENP-A misc HJURP misc DAXX misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc thymic epithelial neoplasms misc thymomas misc histones misc CENP-A misc HJURP misc DAXX misc Biology (General) misc Chemistry
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title	Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms
ctrlnum	(DE-627)DOAJ034547134 (DE-599)DOAJbd6356ee7ab84267b42d9a16ddef88dd
title_full	Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms
author_sort	Georgia Levidou
journal	International Journal of Molecular Sciences
journalStr	International Journal of Molecular Sciences
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lang_code	eng
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author_browse	Georgia Levidou Konstantinos Palamaris Alexandros G. Sykaras Georgios Andreadakis Christos Masaoutis Irene Theochari Penelope Korkolopoulou Dimitra Rontogianni Stamatios Theocharis
container_volume	23
class	QH301-705.5 QD1-999
format_se	Elektronische Aufsätze
author-letter	Georgia Levidou
doi_str_mv	10.3390/ijms23158339
author2-role	verfasserin
title_sort	unraveling the role of histone variant cenp-a and chaperone hjurp expression in thymic epithelial neoplasms
callnumber	QH301-705.5
title_auth	Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms
abstract	Background: Recent advances demonstrate the role of chromatin regulators, including histone variants and histone chaperones, in cancer initiation and progression. Methods: Histone H3K4me3, histone variant centromere protein (CENP-A) and histone chaperones Holliday junction recognition protein (HJURP) as well as DAXX expression were examined immunohistochemically in 95 thymic epithelial tumor (TET) specimens. Our results were compared with the expression profile of DAXX, HJURP and CENP-A in gene expression profiling interactive analysis (GEPIA2). Results: The lymphocyte-poor B3- and C-type TETs were more frequently DAXX negative (<i<p</i< = 0.043). B3 and C-Type TETs showed higher cytoplasmic and nuclear CENP-A (<i<p</i< = 0.007 and <i<p</i< = 0.002) and higher cytoplasmic HJURP H-score (<i<p</i< < 0.001). Higher nuclear CENP-A and cytoplasmic HJURP expression was associated with advanced Masaoka–Koga stage (<i<p</i< = 0.048 and <i<p</i< < 0.001). A positive correlation between HJURP and CENP-A was also observed. The presence of cytoplasmic CENP-A expression was correlated with a favorable overall survival (<i<p</i< = 0.03). CENP-A overexpression in survival analysis of TCGA TETs showed similar results. H3K4me3 expression was not associated with any clinicopathological parameters. Conclusions: Our results suggest a significant interaction between CENP-A and HJURP in TETs. Moreover, we confirmed the presence of a cytoplasmic CENP-A immunolocalization, suggesting also a possible favorable prognostic value of this specific immunostaining pattern.
abstractGer	Background: Recent advances demonstrate the role of chromatin regulators, including histone variants and histone chaperones, in cancer initiation and progression. Methods: Histone H3K4me3, histone variant centromere protein (CENP-A) and histone chaperones Holliday junction recognition protein (HJURP) as well as DAXX expression were examined immunohistochemically in 95 thymic epithelial tumor (TET) specimens. Our results were compared with the expression profile of DAXX, HJURP and CENP-A in gene expression profiling interactive analysis (GEPIA2). Results: The lymphocyte-poor B3- and C-type TETs were more frequently DAXX negative (<i<p</i< = 0.043). B3 and C-Type TETs showed higher cytoplasmic and nuclear CENP-A (<i<p</i< = 0.007 and <i<p</i< = 0.002) and higher cytoplasmic HJURP H-score (<i<p</i< < 0.001). Higher nuclear CENP-A and cytoplasmic HJURP expression was associated with advanced Masaoka–Koga stage (<i<p</i< = 0.048 and <i<p</i< < 0.001). A positive correlation between HJURP and CENP-A was also observed. The presence of cytoplasmic CENP-A expression was correlated with a favorable overall survival (<i<p</i< = 0.03). CENP-A overexpression in survival analysis of TCGA TETs showed similar results. H3K4me3 expression was not associated with any clinicopathological parameters. Conclusions: Our results suggest a significant interaction between CENP-A and HJURP in TETs. Moreover, we confirmed the presence of a cytoplasmic CENP-A immunolocalization, suggesting also a possible favorable prognostic value of this specific immunostaining pattern.
abstract_unstemmed	Background: Recent advances demonstrate the role of chromatin regulators, including histone variants and histone chaperones, in cancer initiation and progression. Methods: Histone H3K4me3, histone variant centromere protein (CENP-A) and histone chaperones Holliday junction recognition protein (HJURP) as well as DAXX expression were examined immunohistochemically in 95 thymic epithelial tumor (TET) specimens. Our results were compared with the expression profile of DAXX, HJURP and CENP-A in gene expression profiling interactive analysis (GEPIA2). Results: The lymphocyte-poor B3- and C-type TETs were more frequently DAXX negative (<i<p</i< = 0.043). B3 and C-Type TETs showed higher cytoplasmic and nuclear CENP-A (<i<p</i< = 0.007 and <i<p</i< = 0.002) and higher cytoplasmic HJURP H-score (<i<p</i< < 0.001). Higher nuclear CENP-A and cytoplasmic HJURP expression was associated with advanced Masaoka–Koga stage (<i<p</i< = 0.048 and <i<p</i< < 0.001). A positive correlation between HJURP and CENP-A was also observed. The presence of cytoplasmic CENP-A expression was correlated with a favorable overall survival (<i<p</i< = 0.03). CENP-A overexpression in survival analysis of TCGA TETs showed similar results. H3K4me3 expression was not associated with any clinicopathological parameters. Conclusions: Our results suggest a significant interaction between CENP-A and HJURP in TETs. Moreover, we confirmed the presence of a cytoplasmic CENP-A immunolocalization, suggesting also a possible favorable prognostic value of this specific immunostaining pattern.
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title_short	Unraveling the Role of Histone Variant CENP-A and Chaperone HJURP Expression in Thymic Epithelial Neoplasms
url	https://doi.org/10.3390/ijms23158339 https://doaj.org/article/bd6356ee7ab84267b42d9a16ddef88dd https://www.mdpi.com/1422-0067/23/15/8339 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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author2	Konstantinos Palamaris Alexandros G. Sykaras Georgios Andreadakis Christos Masaoutis Irene Theochari Penelope Korkolopoulou Dimitra Rontogianni Stamatios Theocharis
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