A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile

Abstract Background Clostridium difficile-associated disease (CDAD) constitutes a great majority of hospital diarrhea cases in industrialized countries and is induced by two types of large toxin molecules: toxin A (TcdA) and toxin B (TcdB). Development of immunotherapeutic approaches, either active...
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Autor*in:	Bao-Zhong Zhang [verfasserIn] Jianpiao Cai [verfasserIn] Bin Yu [verfasserIn] Yanhong Hua [verfasserIn] Candy Choiyi Lau [verfasserIn] Richard Yi-Tsun Tsun Kao [verfasserIn] Kong-Hung Sze [verfasserIn] Kwok-Yung Yuen [verfasserIn] Jian-Dong Huang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	DNA vaccine Clostridium difficile Toxin A (TcdA) Toxin B (TcdB)

Übergeordnetes Werk:	In: BMC Infectious Diseases - BMC, 2003, 16(2016), 1, Seite 8
Übergeordnetes Werk:	volume:16 ; year:2016 ; number:1 ; pages:8

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/s12879-016-1924-1

Katalog-ID:	DOAJ034574263

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520			\|a Abstract Background Clostridium difficile-associated disease (CDAD) constitutes a great majority of hospital diarrhea cases in industrialized countries and is induced by two types of large toxin molecules: toxin A (TcdA) and toxin B (TcdB). Development of immunotherapeutic approaches, either active or passive, has seen a resurgence in recent years. Studies have described vaccine plasmids that express either TcdA and/or TcdB receptor binding domain (RBD). However, the effectiveness of one vector encoding both toxin RBDs against CDAD has not been evaluated. Methods In the study, we constructed highly optimized plasmids to express the receptor binding domains of both TcdA and TcdB from a single vector. The DNA vaccine was evaluated in two animal models for its immunogenicity and protective effects. Results The DNA vaccine induced high levels of serum antibodies to toxin A and/or B and demonstrated neutralizing activity in both in vitro and in vivo systems. In a C. difficile hamster infection model, immunization with the DNA vaccine reduced infection severity and conferred significant protection against a lethal C. difficile strain. Conclusions This study has demonstrated a single plasmid encoding the RBD domains of C. difficile TcdA and TcdB as a DNA vaccine that could provide protection from C. difficile disease.
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allfields	10.1186/s12879-016-1924-1 doi (DE-627)DOAJ034574263 (DE-599)DOAJ7e412c718d36436a9f8e3830c116013e DE-627 ger DE-627 rakwb eng RC109-216 Bao-Zhong Zhang verfasserin aut A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Clostridium difficile-associated disease (CDAD) constitutes a great majority of hospital diarrhea cases in industrialized countries and is induced by two types of large toxin molecules: toxin A (TcdA) and toxin B (TcdB). Development of immunotherapeutic approaches, either active or passive, has seen a resurgence in recent years. Studies have described vaccine plasmids that express either TcdA and/or TcdB receptor binding domain (RBD). However, the effectiveness of one vector encoding both toxin RBDs against CDAD has not been evaluated. Methods In the study, we constructed highly optimized plasmids to express the receptor binding domains of both TcdA and TcdB from a single vector. The DNA vaccine was evaluated in two animal models for its immunogenicity and protective effects. Results The DNA vaccine induced high levels of serum antibodies to toxin A and/or B and demonstrated neutralizing activity in both in vitro and in vivo systems. In a C. difficile hamster infection model, immunization with the DNA vaccine reduced infection severity and conferred significant protection against a lethal C. difficile strain. Conclusions This study has demonstrated a single plasmid encoding the RBD domains of C. difficile TcdA and TcdB as a DNA vaccine that could provide protection from C. difficile disease. DNA vaccine Clostridium difficile Toxin A (TcdA) Toxin B (TcdB) Infectious and parasitic diseases Jianpiao Cai verfasserin aut Bin Yu verfasserin aut Yanhong Hua verfasserin aut Candy Choiyi Lau verfasserin aut Richard Yi-Tsun Tsun Kao verfasserin aut Kong-Hung Sze verfasserin aut Kwok-Yung Yuen verfasserin aut Jian-Dong Huang verfasserin aut In BMC Infectious Diseases BMC, 2003 16(2016), 1, Seite 8 (DE-627)326645381 (DE-600)2041550-3 14712334 nnns volume:16 year:2016 number:1 pages:8 https://doi.org/10.1186/s12879-016-1924-1 kostenfrei https://doaj.org/article/7e412c718d36436a9f8e3830c116013e kostenfrei http://link.springer.com/article/10.1186/s12879-016-1924-1 kostenfrei https://doaj.org/toc/1471-2334 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2016 1 8
spelling	10.1186/s12879-016-1924-1 doi (DE-627)DOAJ034574263 (DE-599)DOAJ7e412c718d36436a9f8e3830c116013e DE-627 ger DE-627 rakwb eng RC109-216 Bao-Zhong Zhang verfasserin aut A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Clostridium difficile-associated disease (CDAD) constitutes a great majority of hospital diarrhea cases in industrialized countries and is induced by two types of large toxin molecules: toxin A (TcdA) and toxin B (TcdB). Development of immunotherapeutic approaches, either active or passive, has seen a resurgence in recent years. Studies have described vaccine plasmids that express either TcdA and/or TcdB receptor binding domain (RBD). However, the effectiveness of one vector encoding both toxin RBDs against CDAD has not been evaluated. Methods In the study, we constructed highly optimized plasmids to express the receptor binding domains of both TcdA and TcdB from a single vector. The DNA vaccine was evaluated in two animal models for its immunogenicity and protective effects. Results The DNA vaccine induced high levels of serum antibodies to toxin A and/or B and demonstrated neutralizing activity in both in vitro and in vivo systems. In a C. difficile hamster infection model, immunization with the DNA vaccine reduced infection severity and conferred significant protection against a lethal C. difficile strain. Conclusions This study has demonstrated a single plasmid encoding the RBD domains of C. difficile TcdA and TcdB as a DNA vaccine that could provide protection from C. difficile disease. DNA vaccine Clostridium difficile Toxin A (TcdA) Toxin B (TcdB) Infectious and parasitic diseases Jianpiao Cai verfasserin aut Bin Yu verfasserin aut Yanhong Hua verfasserin aut Candy Choiyi Lau verfasserin aut Richard Yi-Tsun Tsun Kao verfasserin aut Kong-Hung Sze verfasserin aut Kwok-Yung Yuen verfasserin aut Jian-Dong Huang verfasserin aut In BMC Infectious Diseases BMC, 2003 16(2016), 1, Seite 8 (DE-627)326645381 (DE-600)2041550-3 14712334 nnns volume:16 year:2016 number:1 pages:8 https://doi.org/10.1186/s12879-016-1924-1 kostenfrei https://doaj.org/article/7e412c718d36436a9f8e3830c116013e kostenfrei http://link.springer.com/article/10.1186/s12879-016-1924-1 kostenfrei https://doaj.org/toc/1471-2334 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2016 1 8
allfields_unstemmed	10.1186/s12879-016-1924-1 doi (DE-627)DOAJ034574263 (DE-599)DOAJ7e412c718d36436a9f8e3830c116013e DE-627 ger DE-627 rakwb eng RC109-216 Bao-Zhong Zhang verfasserin aut A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Clostridium difficile-associated disease (CDAD) constitutes a great majority of hospital diarrhea cases in industrialized countries and is induced by two types of large toxin molecules: toxin A (TcdA) and toxin B (TcdB). Development of immunotherapeutic approaches, either active or passive, has seen a resurgence in recent years. Studies have described vaccine plasmids that express either TcdA and/or TcdB receptor binding domain (RBD). However, the effectiveness of one vector encoding both toxin RBDs against CDAD has not been evaluated. Methods In the study, we constructed highly optimized plasmids to express the receptor binding domains of both TcdA and TcdB from a single vector. The DNA vaccine was evaluated in two animal models for its immunogenicity and protective effects. Results The DNA vaccine induced high levels of serum antibodies to toxin A and/or B and demonstrated neutralizing activity in both in vitro and in vivo systems. In a C. difficile hamster infection model, immunization with the DNA vaccine reduced infection severity and conferred significant protection against a lethal C. difficile strain. Conclusions This study has demonstrated a single plasmid encoding the RBD domains of C. difficile TcdA and TcdB as a DNA vaccine that could provide protection from C. difficile disease. DNA vaccine Clostridium difficile Toxin A (TcdA) Toxin B (TcdB) Infectious and parasitic diseases Jianpiao Cai verfasserin aut Bin Yu verfasserin aut Yanhong Hua verfasserin aut Candy Choiyi Lau verfasserin aut Richard Yi-Tsun Tsun Kao verfasserin aut Kong-Hung Sze verfasserin aut Kwok-Yung Yuen verfasserin aut Jian-Dong Huang verfasserin aut In BMC Infectious Diseases BMC, 2003 16(2016), 1, Seite 8 (DE-627)326645381 (DE-600)2041550-3 14712334 nnns volume:16 year:2016 number:1 pages:8 https://doi.org/10.1186/s12879-016-1924-1 kostenfrei https://doaj.org/article/7e412c718d36436a9f8e3830c116013e kostenfrei http://link.springer.com/article/10.1186/s12879-016-1924-1 kostenfrei https://doaj.org/toc/1471-2334 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2016 1 8
allfieldsGer	10.1186/s12879-016-1924-1 doi (DE-627)DOAJ034574263 (DE-599)DOAJ7e412c718d36436a9f8e3830c116013e DE-627 ger DE-627 rakwb eng RC109-216 Bao-Zhong Zhang verfasserin aut A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Clostridium difficile-associated disease (CDAD) constitutes a great majority of hospital diarrhea cases in industrialized countries and is induced by two types of large toxin molecules: toxin A (TcdA) and toxin B (TcdB). Development of immunotherapeutic approaches, either active or passive, has seen a resurgence in recent years. Studies have described vaccine plasmids that express either TcdA and/or TcdB receptor binding domain (RBD). However, the effectiveness of one vector encoding both toxin RBDs against CDAD has not been evaluated. Methods In the study, we constructed highly optimized plasmids to express the receptor binding domains of both TcdA and TcdB from a single vector. The DNA vaccine was evaluated in two animal models for its immunogenicity and protective effects. Results The DNA vaccine induced high levels of serum antibodies to toxin A and/or B and demonstrated neutralizing activity in both in vitro and in vivo systems. In a C. difficile hamster infection model, immunization with the DNA vaccine reduced infection severity and conferred significant protection against a lethal C. difficile strain. Conclusions This study has demonstrated a single plasmid encoding the RBD domains of C. difficile TcdA and TcdB as a DNA vaccine that could provide protection from C. difficile disease. DNA vaccine Clostridium difficile Toxin A (TcdA) Toxin B (TcdB) Infectious and parasitic diseases Jianpiao Cai verfasserin aut Bin Yu verfasserin aut Yanhong Hua verfasserin aut Candy Choiyi Lau verfasserin aut Richard Yi-Tsun Tsun Kao verfasserin aut Kong-Hung Sze verfasserin aut Kwok-Yung Yuen verfasserin aut Jian-Dong Huang verfasserin aut In BMC Infectious Diseases BMC, 2003 16(2016), 1, Seite 8 (DE-627)326645381 (DE-600)2041550-3 14712334 nnns volume:16 year:2016 number:1 pages:8 https://doi.org/10.1186/s12879-016-1924-1 kostenfrei https://doaj.org/article/7e412c718d36436a9f8e3830c116013e kostenfrei http://link.springer.com/article/10.1186/s12879-016-1924-1 kostenfrei https://doaj.org/toc/1471-2334 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2016 1 8
allfieldsSound	10.1186/s12879-016-1924-1 doi (DE-627)DOAJ034574263 (DE-599)DOAJ7e412c718d36436a9f8e3830c116013e DE-627 ger DE-627 rakwb eng RC109-216 Bao-Zhong Zhang verfasserin aut A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Clostridium difficile-associated disease (CDAD) constitutes a great majority of hospital diarrhea cases in industrialized countries and is induced by two types of large toxin molecules: toxin A (TcdA) and toxin B (TcdB). Development of immunotherapeutic approaches, either active or passive, has seen a resurgence in recent years. Studies have described vaccine plasmids that express either TcdA and/or TcdB receptor binding domain (RBD). However, the effectiveness of one vector encoding both toxin RBDs against CDAD has not been evaluated. Methods In the study, we constructed highly optimized plasmids to express the receptor binding domains of both TcdA and TcdB from a single vector. The DNA vaccine was evaluated in two animal models for its immunogenicity and protective effects. Results The DNA vaccine induced high levels of serum antibodies to toxin A and/or B and demonstrated neutralizing activity in both in vitro and in vivo systems. In a C. difficile hamster infection model, immunization with the DNA vaccine reduced infection severity and conferred significant protection against a lethal C. difficile strain. Conclusions This study has demonstrated a single plasmid encoding the RBD domains of C. difficile TcdA and TcdB as a DNA vaccine that could provide protection from C. difficile disease. DNA vaccine Clostridium difficile Toxin A (TcdA) Toxin B (TcdB) Infectious and parasitic diseases Jianpiao Cai verfasserin aut Bin Yu verfasserin aut Yanhong Hua verfasserin aut Candy Choiyi Lau verfasserin aut Richard Yi-Tsun Tsun Kao verfasserin aut Kong-Hung Sze verfasserin aut Kwok-Yung Yuen verfasserin aut Jian-Dong Huang verfasserin aut In BMC Infectious Diseases BMC, 2003 16(2016), 1, Seite 8 (DE-627)326645381 (DE-600)2041550-3 14712334 nnns volume:16 year:2016 number:1 pages:8 https://doi.org/10.1186/s12879-016-1924-1 kostenfrei https://doaj.org/article/7e412c718d36436a9f8e3830c116013e kostenfrei http://link.springer.com/article/10.1186/s12879-016-1924-1 kostenfrei https://doaj.org/toc/1471-2334 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2016 1 8
language	English
source	In BMC Infectious Diseases 16(2016), 1, Seite 8 volume:16 year:2016 number:1 pages:8
sourceStr	In BMC Infectious Diseases 16(2016), 1, Seite 8 volume:16 year:2016 number:1 pages:8
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	DNA vaccine Clostridium difficile Toxin A (TcdA) Toxin B (TcdB) Infectious and parasitic diseases
isfreeaccess_bool	true
container_title	BMC Infectious Diseases
authorswithroles_txt_mv	Bao-Zhong Zhang @@aut@@ Jianpiao Cai @@aut@@ Bin Yu @@aut@@ Yanhong Hua @@aut@@ Candy Choiyi Lau @@aut@@ Richard Yi-Tsun Tsun Kao @@aut@@ Kong-Hung Sze @@aut@@ Kwok-Yung Yuen @@aut@@ Jian-Dong Huang @@aut@@
publishDateDaySort_date	2016-01-01T00:00:00Z
hierarchy_top_id	326645381
id	DOAJ034574263
language_de	englisch
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callnumber-first	R - Medicine
author	Bao-Zhong Zhang
spellingShingle	Bao-Zhong Zhang misc RC109-216 misc DNA vaccine misc Clostridium difficile misc Toxin A (TcdA) misc Toxin B (TcdB) misc Infectious and parasitic diseases A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile
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topic_title	RC109-216 A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile DNA vaccine Clostridium difficile Toxin A (TcdA) Toxin B (TcdB)
topic	misc RC109-216 misc DNA vaccine misc Clostridium difficile misc Toxin A (TcdA) misc Toxin B (TcdB) misc Infectious and parasitic diseases
topic_unstemmed	misc RC109-216 misc DNA vaccine misc Clostridium difficile misc Toxin A (TcdA) misc Toxin B (TcdB) misc Infectious and parasitic diseases
topic_browse	misc RC109-216 misc DNA vaccine misc Clostridium difficile misc Toxin A (TcdA) misc Toxin B (TcdB) misc Infectious and parasitic diseases
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title	A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile
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title_full	A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile
author_sort	Bao-Zhong Zhang
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author_browse	Bao-Zhong Zhang Jianpiao Cai Bin Yu Yanhong Hua Candy Choiyi Lau Richard Yi-Tsun Tsun Kao Kong-Hung Sze Kwok-Yung Yuen Jian-Dong Huang
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title_sort	dna vaccine targeting tcda and tcdb induces protective immunity against clostridium difficile
callnumber	RC109-216
title_auth	A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile
abstract	Abstract Background Clostridium difficile-associated disease (CDAD) constitutes a great majority of hospital diarrhea cases in industrialized countries and is induced by two types of large toxin molecules: toxin A (TcdA) and toxin B (TcdB). Development of immunotherapeutic approaches, either active or passive, has seen a resurgence in recent years. Studies have described vaccine plasmids that express either TcdA and/or TcdB receptor binding domain (RBD). However, the effectiveness of one vector encoding both toxin RBDs against CDAD has not been evaluated. Methods In the study, we constructed highly optimized plasmids to express the receptor binding domains of both TcdA and TcdB from a single vector. The DNA vaccine was evaluated in two animal models for its immunogenicity and protective effects. Results The DNA vaccine induced high levels of serum antibodies to toxin A and/or B and demonstrated neutralizing activity in both in vitro and in vivo systems. In a C. difficile hamster infection model, immunization with the DNA vaccine reduced infection severity and conferred significant protection against a lethal C. difficile strain. Conclusions This study has demonstrated a single plasmid encoding the RBD domains of C. difficile TcdA and TcdB as a DNA vaccine that could provide protection from C. difficile disease.
abstractGer	Abstract Background Clostridium difficile-associated disease (CDAD) constitutes a great majority of hospital diarrhea cases in industrialized countries and is induced by two types of large toxin molecules: toxin A (TcdA) and toxin B (TcdB). Development of immunotherapeutic approaches, either active or passive, has seen a resurgence in recent years. Studies have described vaccine plasmids that express either TcdA and/or TcdB receptor binding domain (RBD). However, the effectiveness of one vector encoding both toxin RBDs against CDAD has not been evaluated. Methods In the study, we constructed highly optimized plasmids to express the receptor binding domains of both TcdA and TcdB from a single vector. The DNA vaccine was evaluated in two animal models for its immunogenicity and protective effects. Results The DNA vaccine induced high levels of serum antibodies to toxin A and/or B and demonstrated neutralizing activity in both in vitro and in vivo systems. In a C. difficile hamster infection model, immunization with the DNA vaccine reduced infection severity and conferred significant protection against a lethal C. difficile strain. Conclusions This study has demonstrated a single plasmid encoding the RBD domains of C. difficile TcdA and TcdB as a DNA vaccine that could provide protection from C. difficile disease.
abstract_unstemmed	Abstract Background Clostridium difficile-associated disease (CDAD) constitutes a great majority of hospital diarrhea cases in industrialized countries and is induced by two types of large toxin molecules: toxin A (TcdA) and toxin B (TcdB). Development of immunotherapeutic approaches, either active or passive, has seen a resurgence in recent years. Studies have described vaccine plasmids that express either TcdA and/or TcdB receptor binding domain (RBD). However, the effectiveness of one vector encoding both toxin RBDs against CDAD has not been evaluated. Methods In the study, we constructed highly optimized plasmids to express the receptor binding domains of both TcdA and TcdB from a single vector. The DNA vaccine was evaluated in two animal models for its immunogenicity and protective effects. Results The DNA vaccine induced high levels of serum antibodies to toxin A and/or B and demonstrated neutralizing activity in both in vitro and in vivo systems. In a C. difficile hamster infection model, immunization with the DNA vaccine reduced infection severity and conferred significant protection against a lethal C. difficile strain. Conclusions This study has demonstrated a single plasmid encoding the RBD domains of C. difficile TcdA and TcdB as a DNA vaccine that could provide protection from C. difficile disease.
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container_issue	1
title_short	A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile
url	https://doi.org/10.1186/s12879-016-1924-1 https://doaj.org/article/7e412c718d36436a9f8e3830c116013e http://link.springer.com/article/10.1186/s12879-016-1924-1 https://doaj.org/toc/1471-2334
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A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile

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