Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in <i<Myzus persicae</i<

Uridine diphosphate (UDP)-glycosyltransferases (UGTs) are major phase II detoxification enzymes involved in glycosylation of lipophilic endobiotics and xenobiotics, including phytoalexins. Nicotine, one of the most abundant secondary plant metabolites in tobacco, is highly toxic to herbivorous insec...
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Autor*in:	Yiou Pan [verfasserIn] Pengjun Xu [verfasserIn] Xiaochun Zeng [verfasserIn] Xuemei Liu [verfasserIn] Qingli Shang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	UDP-glucuronosyltransferase host adaptation nicotine tolerance

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 20(2019), 15, p 3637
Übergeordnetes Werk:	volume:20 ; year:2019 ; number:15, p 3637

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/ijms20153637

Katalog-ID:	DOAJ034750479

Internformat


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520			\|a Uridine diphosphate (UDP)-glycosyltransferases (UGTs) are major phase II detoxification enzymes involved in glycosylation of lipophilic endobiotics and xenobiotics, including phytoalexins. Nicotine, one of the most abundant secondary plant metabolites in tobacco, is highly toxic to herbivorous insects. Plant-herbivore competition is the major impetus for the evolution of large superfamilies of <i<UGTs</i< and other detoxification enzymes. However, UGT functions in green peach aphid (<i<Myzus persicae</i<) adaptation are unknown. In this study, we show that UGT inhibitors (sulfinpyrazone and 5-nitrouracil) significantly increased nicotine toxicity in <i<M. persicae nicotianae</i<, suggesting that UGTs may be involved in nicotine tolerance. In total, 101 <i<UGT</i< transcripts identified in the <i<M. persicae</i< genome/transcriptome were renamed according to the UGT Nomenclature Committee guidelines and grouped into 11 families, UGT329, UGT330, UGT339, UGT341−UGT345, and UGT348−UGT350, with UGT344 containing the most (57). Ten <i<UGTs</i< (<i<UGT330A3</i<, <i<UGT339A2</i<, <i<UGT341A6</i<, <i<UGT342B3</i<, <i<UGT343C3</i<, <i<UGT344D5</i<, <i<UGT344D8</i<, <i<UGT348A3</i<, <i<UGT349A3</i<, and <i<UGT350A3</i<) were highly expressed in <i<M. persicae nicotianae</i< compared to <i<M. persicae</i< sensu stricto. Knockdown of four UGTs (<i<UGT330A3</i<, <i<UGT344D5</i<, <i<UGT348A3</i<, and <i<UGT349A3</i<) significantly increased <i<M. persicae nicotianae</i< sensitivity to nicotine, suggesting that <i<UGT</i< expression in this subspecies may be associated with nicotine tolerance and thus host adaptation. This study reveals possible <i<UGTs</i< relevant to nicotine adaptation in tobacco-consuming <i<M. persicae nicotianae</i<, and the findings will facilitate further validation of the roles of these <i<UGTs</i< in nicotine tolerance.
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allfields	10.3390/ijms20153637 doi (DE-627)DOAJ034750479 (DE-599)DOAJ310e24e47ac64fa1b8ab0dcfeb85e336 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Yiou Pan verfasserin aut Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in <i<Myzus persicae</i< 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Uridine diphosphate (UDP)-glycosyltransferases (UGTs) are major phase II detoxification enzymes involved in glycosylation of lipophilic endobiotics and xenobiotics, including phytoalexins. Nicotine, one of the most abundant secondary plant metabolites in tobacco, is highly toxic to herbivorous insects. Plant-herbivore competition is the major impetus for the evolution of large superfamilies of <i<UGTs</i< and other detoxification enzymes. However, UGT functions in green peach aphid (<i<Myzus persicae</i<) adaptation are unknown. In this study, we show that UGT inhibitors (sulfinpyrazone and 5-nitrouracil) significantly increased nicotine toxicity in <i<M. persicae nicotianae</i<, suggesting that UGTs may be involved in nicotine tolerance. In total, 101 <i<UGT</i< transcripts identified in the <i<M. persicae</i< genome/transcriptome were renamed according to the UGT Nomenclature Committee guidelines and grouped into 11 families, UGT329, UGT330, UGT339, UGT341−UGT345, and UGT348−UGT350, with UGT344 containing the most (57). Ten <i<UGTs</i< (<i<UGT330A3</i<, <i<UGT339A2</i<, <i<UGT341A6</i<, <i<UGT342B3</i<, <i<UGT343C3</i<, <i<UGT344D5</i<, <i<UGT344D8</i<, <i<UGT348A3</i<, <i<UGT349A3</i<, and <i<UGT350A3</i<) were highly expressed in <i<M. persicae nicotianae</i< compared to <i<M. persicae</i< sensu stricto. Knockdown of four UGTs (<i<UGT330A3</i<, <i<UGT344D5</i<, <i<UGT348A3</i<, and <i<UGT349A3</i<) significantly increased <i<M. persicae nicotianae</i< sensitivity to nicotine, suggesting that <i<UGT</i< expression in this subspecies may be associated with nicotine tolerance and thus host adaptation. This study reveals possible <i<UGTs</i< relevant to nicotine adaptation in tobacco-consuming <i<M. persicae nicotianae</i<, and the findings will facilitate further validation of the roles of these <i<UGTs</i< in nicotine tolerance. UDP-glucuronosyltransferase host adaptation <i<Myzus persicae</i< nicotine tolerance Biology (General) Chemistry Pengjun Xu verfasserin aut Xiaochun Zeng verfasserin aut Xuemei Liu verfasserin aut Qingli Shang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 15, p 3637 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:15, p 3637 https://doi.org/10.3390/ijms20153637 kostenfrei https://doaj.org/article/310e24e47ac64fa1b8ab0dcfeb85e336 kostenfrei https://www.mdpi.com/1422-0067/20/15/3637 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 15, p 3637
spelling	10.3390/ijms20153637 doi (DE-627)DOAJ034750479 (DE-599)DOAJ310e24e47ac64fa1b8ab0dcfeb85e336 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Yiou Pan verfasserin aut Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in <i<Myzus persicae</i< 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Uridine diphosphate (UDP)-glycosyltransferases (UGTs) are major phase II detoxification enzymes involved in glycosylation of lipophilic endobiotics and xenobiotics, including phytoalexins. Nicotine, one of the most abundant secondary plant metabolites in tobacco, is highly toxic to herbivorous insects. Plant-herbivore competition is the major impetus for the evolution of large superfamilies of <i<UGTs</i< and other detoxification enzymes. However, UGT functions in green peach aphid (<i<Myzus persicae</i<) adaptation are unknown. In this study, we show that UGT inhibitors (sulfinpyrazone and 5-nitrouracil) significantly increased nicotine toxicity in <i<M. persicae nicotianae</i<, suggesting that UGTs may be involved in nicotine tolerance. In total, 101 <i<UGT</i< transcripts identified in the <i<M. persicae</i< genome/transcriptome were renamed according to the UGT Nomenclature Committee guidelines and grouped into 11 families, UGT329, UGT330, UGT339, UGT341−UGT345, and UGT348−UGT350, with UGT344 containing the most (57). Ten <i<UGTs</i< (<i<UGT330A3</i<, <i<UGT339A2</i<, <i<UGT341A6</i<, <i<UGT342B3</i<, <i<UGT343C3</i<, <i<UGT344D5</i<, <i<UGT344D8</i<, <i<UGT348A3</i<, <i<UGT349A3</i<, and <i<UGT350A3</i<) were highly expressed in <i<M. persicae nicotianae</i< compared to <i<M. persicae</i< sensu stricto. Knockdown of four UGTs (<i<UGT330A3</i<, <i<UGT344D5</i<, <i<UGT348A3</i<, and <i<UGT349A3</i<) significantly increased <i<M. persicae nicotianae</i< sensitivity to nicotine, suggesting that <i<UGT</i< expression in this subspecies may be associated with nicotine tolerance and thus host adaptation. This study reveals possible <i<UGTs</i< relevant to nicotine adaptation in tobacco-consuming <i<M. persicae nicotianae</i<, and the findings will facilitate further validation of the roles of these <i<UGTs</i< in nicotine tolerance. UDP-glucuronosyltransferase host adaptation <i<Myzus persicae</i< nicotine tolerance Biology (General) Chemistry Pengjun Xu verfasserin aut Xiaochun Zeng verfasserin aut Xuemei Liu verfasserin aut Qingli Shang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 15, p 3637 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:15, p 3637 https://doi.org/10.3390/ijms20153637 kostenfrei https://doaj.org/article/310e24e47ac64fa1b8ab0dcfeb85e336 kostenfrei https://www.mdpi.com/1422-0067/20/15/3637 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 15, p 3637
allfields_unstemmed	10.3390/ijms20153637 doi (DE-627)DOAJ034750479 (DE-599)DOAJ310e24e47ac64fa1b8ab0dcfeb85e336 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Yiou Pan verfasserin aut Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in <i<Myzus persicae</i< 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Uridine diphosphate (UDP)-glycosyltransferases (UGTs) are major phase II detoxification enzymes involved in glycosylation of lipophilic endobiotics and xenobiotics, including phytoalexins. Nicotine, one of the most abundant secondary plant metabolites in tobacco, is highly toxic to herbivorous insects. Plant-herbivore competition is the major impetus for the evolution of large superfamilies of <i<UGTs</i< and other detoxification enzymes. However, UGT functions in green peach aphid (<i<Myzus persicae</i<) adaptation are unknown. In this study, we show that UGT inhibitors (sulfinpyrazone and 5-nitrouracil) significantly increased nicotine toxicity in <i<M. persicae nicotianae</i<, suggesting that UGTs may be involved in nicotine tolerance. In total, 101 <i<UGT</i< transcripts identified in the <i<M. persicae</i< genome/transcriptome were renamed according to the UGT Nomenclature Committee guidelines and grouped into 11 families, UGT329, UGT330, UGT339, UGT341−UGT345, and UGT348−UGT350, with UGT344 containing the most (57). Ten <i<UGTs</i< (<i<UGT330A3</i<, <i<UGT339A2</i<, <i<UGT341A6</i<, <i<UGT342B3</i<, <i<UGT343C3</i<, <i<UGT344D5</i<, <i<UGT344D8</i<, <i<UGT348A3</i<, <i<UGT349A3</i<, and <i<UGT350A3</i<) were highly expressed in <i<M. persicae nicotianae</i< compared to <i<M. persicae</i< sensu stricto. Knockdown of four UGTs (<i<UGT330A3</i<, <i<UGT344D5</i<, <i<UGT348A3</i<, and <i<UGT349A3</i<) significantly increased <i<M. persicae nicotianae</i< sensitivity to nicotine, suggesting that <i<UGT</i< expression in this subspecies may be associated with nicotine tolerance and thus host adaptation. This study reveals possible <i<UGTs</i< relevant to nicotine adaptation in tobacco-consuming <i<M. persicae nicotianae</i<, and the findings will facilitate further validation of the roles of these <i<UGTs</i< in nicotine tolerance. UDP-glucuronosyltransferase host adaptation <i<Myzus persicae</i< nicotine tolerance Biology (General) Chemistry Pengjun Xu verfasserin aut Xiaochun Zeng verfasserin aut Xuemei Liu verfasserin aut Qingli Shang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 15, p 3637 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:15, p 3637 https://doi.org/10.3390/ijms20153637 kostenfrei https://doaj.org/article/310e24e47ac64fa1b8ab0dcfeb85e336 kostenfrei https://www.mdpi.com/1422-0067/20/15/3637 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 15, p 3637
allfieldsGer	10.3390/ijms20153637 doi (DE-627)DOAJ034750479 (DE-599)DOAJ310e24e47ac64fa1b8ab0dcfeb85e336 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Yiou Pan verfasserin aut Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in <i<Myzus persicae</i< 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Uridine diphosphate (UDP)-glycosyltransferases (UGTs) are major phase II detoxification enzymes involved in glycosylation of lipophilic endobiotics and xenobiotics, including phytoalexins. Nicotine, one of the most abundant secondary plant metabolites in tobacco, is highly toxic to herbivorous insects. Plant-herbivore competition is the major impetus for the evolution of large superfamilies of <i<UGTs</i< and other detoxification enzymes. However, UGT functions in green peach aphid (<i<Myzus persicae</i<) adaptation are unknown. In this study, we show that UGT inhibitors (sulfinpyrazone and 5-nitrouracil) significantly increased nicotine toxicity in <i<M. persicae nicotianae</i<, suggesting that UGTs may be involved in nicotine tolerance. In total, 101 <i<UGT</i< transcripts identified in the <i<M. persicae</i< genome/transcriptome were renamed according to the UGT Nomenclature Committee guidelines and grouped into 11 families, UGT329, UGT330, UGT339, UGT341−UGT345, and UGT348−UGT350, with UGT344 containing the most (57). Ten <i<UGTs</i< (<i<UGT330A3</i<, <i<UGT339A2</i<, <i<UGT341A6</i<, <i<UGT342B3</i<, <i<UGT343C3</i<, <i<UGT344D5</i<, <i<UGT344D8</i<, <i<UGT348A3</i<, <i<UGT349A3</i<, and <i<UGT350A3</i<) were highly expressed in <i<M. persicae nicotianae</i< compared to <i<M. persicae</i< sensu stricto. Knockdown of four UGTs (<i<UGT330A3</i<, <i<UGT344D5</i<, <i<UGT348A3</i<, and <i<UGT349A3</i<) significantly increased <i<M. persicae nicotianae</i< sensitivity to nicotine, suggesting that <i<UGT</i< expression in this subspecies may be associated with nicotine tolerance and thus host adaptation. This study reveals possible <i<UGTs</i< relevant to nicotine adaptation in tobacco-consuming <i<M. persicae nicotianae</i<, and the findings will facilitate further validation of the roles of these <i<UGTs</i< in nicotine tolerance. UDP-glucuronosyltransferase host adaptation <i<Myzus persicae</i< nicotine tolerance Biology (General) Chemistry Pengjun Xu verfasserin aut Xiaochun Zeng verfasserin aut Xuemei Liu verfasserin aut Qingli Shang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 15, p 3637 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:15, p 3637 https://doi.org/10.3390/ijms20153637 kostenfrei https://doaj.org/article/310e24e47ac64fa1b8ab0dcfeb85e336 kostenfrei https://www.mdpi.com/1422-0067/20/15/3637 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 15, p 3637
allfieldsSound	10.3390/ijms20153637 doi (DE-627)DOAJ034750479 (DE-599)DOAJ310e24e47ac64fa1b8ab0dcfeb85e336 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Yiou Pan verfasserin aut Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in <i<Myzus persicae</i< 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Uridine diphosphate (UDP)-glycosyltransferases (UGTs) are major phase II detoxification enzymes involved in glycosylation of lipophilic endobiotics and xenobiotics, including phytoalexins. Nicotine, one of the most abundant secondary plant metabolites in tobacco, is highly toxic to herbivorous insects. Plant-herbivore competition is the major impetus for the evolution of large superfamilies of <i<UGTs</i< and other detoxification enzymes. However, UGT functions in green peach aphid (<i<Myzus persicae</i<) adaptation are unknown. In this study, we show that UGT inhibitors (sulfinpyrazone and 5-nitrouracil) significantly increased nicotine toxicity in <i<M. persicae nicotianae</i<, suggesting that UGTs may be involved in nicotine tolerance. In total, 101 <i<UGT</i< transcripts identified in the <i<M. persicae</i< genome/transcriptome were renamed according to the UGT Nomenclature Committee guidelines and grouped into 11 families, UGT329, UGT330, UGT339, UGT341−UGT345, and UGT348−UGT350, with UGT344 containing the most (57). Ten <i<UGTs</i< (<i<UGT330A3</i<, <i<UGT339A2</i<, <i<UGT341A6</i<, <i<UGT342B3</i<, <i<UGT343C3</i<, <i<UGT344D5</i<, <i<UGT344D8</i<, <i<UGT348A3</i<, <i<UGT349A3</i<, and <i<UGT350A3</i<) were highly expressed in <i<M. persicae nicotianae</i< compared to <i<M. persicae</i< sensu stricto. Knockdown of four UGTs (<i<UGT330A3</i<, <i<UGT344D5</i<, <i<UGT348A3</i<, and <i<UGT349A3</i<) significantly increased <i<M. persicae nicotianae</i< sensitivity to nicotine, suggesting that <i<UGT</i< expression in this subspecies may be associated with nicotine tolerance and thus host adaptation. This study reveals possible <i<UGTs</i< relevant to nicotine adaptation in tobacco-consuming <i<M. persicae nicotianae</i<, and the findings will facilitate further validation of the roles of these <i<UGTs</i< in nicotine tolerance. UDP-glucuronosyltransferase host adaptation <i<Myzus persicae</i< nicotine tolerance Biology (General) Chemistry Pengjun Xu verfasserin aut Xiaochun Zeng verfasserin aut Xuemei Liu verfasserin aut Qingli Shang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 15, p 3637 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:15, p 3637 https://doi.org/10.3390/ijms20153637 kostenfrei https://doaj.org/article/310e24e47ac64fa1b8ab0dcfeb85e336 kostenfrei https://www.mdpi.com/1422-0067/20/15/3637 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 15, p 3637
language	English
source	In International Journal of Molecular Sciences 20(2019), 15, p 3637 volume:20 year:2019 number:15, p 3637
sourceStr	In International Journal of Molecular Sciences 20(2019), 15, p 3637 volume:20 year:2019 number:15, p 3637
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	UDP-glucuronosyltransferase host adaptation <i<Myzus persicae</i< nicotine tolerance Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Yiou Pan @@aut@@ Pengjun Xu @@aut@@ Xiaochun Zeng @@aut@@ Xuemei Liu @@aut@@ Qingli Shang @@aut@@
publishDateDaySort_date	2019-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ034750479
language_de	englisch
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callnumber-first	Q - Science
author	Yiou Pan
spellingShingle	Yiou Pan misc QH301-705.5 misc QD1-999 misc UDP-glucuronosyltransferase misc host adaptation misc <i<Myzus persicae</i< misc nicotine tolerance misc Biology (General) misc Chemistry Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in <i<Myzus persicae</i<
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topic_title	QH301-705.5 QD1-999 Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in <i<Myzus persicae</i< UDP-glucuronosyltransferase host adaptation <i<Myzus persicae</i< nicotine tolerance
topic	misc QH301-705.5 misc QD1-999 misc UDP-glucuronosyltransferase misc host adaptation misc <i<Myzus persicae</i< misc nicotine tolerance misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc UDP-glucuronosyltransferase misc host adaptation misc <i<Myzus persicae</i< misc nicotine tolerance misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc UDP-glucuronosyltransferase misc host adaptation misc <i<Myzus persicae</i< misc nicotine tolerance misc Biology (General) misc Chemistry
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title	Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in <i<Myzus persicae</i<
ctrlnum	(DE-627)DOAJ034750479 (DE-599)DOAJ310e24e47ac64fa1b8ab0dcfeb85e336
title_full	Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in <i<Myzus persicae</i<
author_sort	Yiou Pan
journal	International Journal of Molecular Sciences
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author_browse	Yiou Pan Pengjun Xu Xiaochun Zeng Xuemei Liu Qingli Shang
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author-letter	Yiou Pan
doi_str_mv	10.3390/ijms20153637
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title_sort	characterization of udp-glucuronosyltransferases and the potential contribution to nicotine tolerance in <i<myzus persicae</i<
callnumber	QH301-705.5
title_auth	Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in <i<Myzus persicae</i<
abstract	Uridine diphosphate (UDP)-glycosyltransferases (UGTs) are major phase II detoxification enzymes involved in glycosylation of lipophilic endobiotics and xenobiotics, including phytoalexins. Nicotine, one of the most abundant secondary plant metabolites in tobacco, is highly toxic to herbivorous insects. Plant-herbivore competition is the major impetus for the evolution of large superfamilies of <i<UGTs</i< and other detoxification enzymes. However, UGT functions in green peach aphid (<i<Myzus persicae</i<) adaptation are unknown. In this study, we show that UGT inhibitors (sulfinpyrazone and 5-nitrouracil) significantly increased nicotine toxicity in <i<M. persicae nicotianae</i<, suggesting that UGTs may be involved in nicotine tolerance. In total, 101 <i<UGT</i< transcripts identified in the <i<M. persicae</i< genome/transcriptome were renamed according to the UGT Nomenclature Committee guidelines and grouped into 11 families, UGT329, UGT330, UGT339, UGT341−UGT345, and UGT348−UGT350, with UGT344 containing the most (57). Ten <i<UGTs</i< (<i<UGT330A3</i<, <i<UGT339A2</i<, <i<UGT341A6</i<, <i<UGT342B3</i<, <i<UGT343C3</i<, <i<UGT344D5</i<, <i<UGT344D8</i<, <i<UGT348A3</i<, <i<UGT349A3</i<, and <i<UGT350A3</i<) were highly expressed in <i<M. persicae nicotianae</i< compared to <i<M. persicae</i< sensu stricto. Knockdown of four UGTs (<i<UGT330A3</i<, <i<UGT344D5</i<, <i<UGT348A3</i<, and <i<UGT349A3</i<) significantly increased <i<M. persicae nicotianae</i< sensitivity to nicotine, suggesting that <i<UGT</i< expression in this subspecies may be associated with nicotine tolerance and thus host adaptation. This study reveals possible <i<UGTs</i< relevant to nicotine adaptation in tobacco-consuming <i<M. persicae nicotianae</i<, and the findings will facilitate further validation of the roles of these <i<UGTs</i< in nicotine tolerance.
abstractGer	Uridine diphosphate (UDP)-glycosyltransferases (UGTs) are major phase II detoxification enzymes involved in glycosylation of lipophilic endobiotics and xenobiotics, including phytoalexins. Nicotine, one of the most abundant secondary plant metabolites in tobacco, is highly toxic to herbivorous insects. Plant-herbivore competition is the major impetus for the evolution of large superfamilies of <i<UGTs</i< and other detoxification enzymes. However, UGT functions in green peach aphid (<i<Myzus persicae</i<) adaptation are unknown. In this study, we show that UGT inhibitors (sulfinpyrazone and 5-nitrouracil) significantly increased nicotine toxicity in <i<M. persicae nicotianae</i<, suggesting that UGTs may be involved in nicotine tolerance. In total, 101 <i<UGT</i< transcripts identified in the <i<M. persicae</i< genome/transcriptome were renamed according to the UGT Nomenclature Committee guidelines and grouped into 11 families, UGT329, UGT330, UGT339, UGT341−UGT345, and UGT348−UGT350, with UGT344 containing the most (57). Ten <i<UGTs</i< (<i<UGT330A3</i<, <i<UGT339A2</i<, <i<UGT341A6</i<, <i<UGT342B3</i<, <i<UGT343C3</i<, <i<UGT344D5</i<, <i<UGT344D8</i<, <i<UGT348A3</i<, <i<UGT349A3</i<, and <i<UGT350A3</i<) were highly expressed in <i<M. persicae nicotianae</i< compared to <i<M. persicae</i< sensu stricto. Knockdown of four UGTs (<i<UGT330A3</i<, <i<UGT344D5</i<, <i<UGT348A3</i<, and <i<UGT349A3</i<) significantly increased <i<M. persicae nicotianae</i< sensitivity to nicotine, suggesting that <i<UGT</i< expression in this subspecies may be associated with nicotine tolerance and thus host adaptation. This study reveals possible <i<UGTs</i< relevant to nicotine adaptation in tobacco-consuming <i<M. persicae nicotianae</i<, and the findings will facilitate further validation of the roles of these <i<UGTs</i< in nicotine tolerance.
abstract_unstemmed	Uridine diphosphate (UDP)-glycosyltransferases (UGTs) are major phase II detoxification enzymes involved in glycosylation of lipophilic endobiotics and xenobiotics, including phytoalexins. Nicotine, one of the most abundant secondary plant metabolites in tobacco, is highly toxic to herbivorous insects. Plant-herbivore competition is the major impetus for the evolution of large superfamilies of <i<UGTs</i< and other detoxification enzymes. However, UGT functions in green peach aphid (<i<Myzus persicae</i<) adaptation are unknown. In this study, we show that UGT inhibitors (sulfinpyrazone and 5-nitrouracil) significantly increased nicotine toxicity in <i<M. persicae nicotianae</i<, suggesting that UGTs may be involved in nicotine tolerance. In total, 101 <i<UGT</i< transcripts identified in the <i<M. persicae</i< genome/transcriptome were renamed according to the UGT Nomenclature Committee guidelines and grouped into 11 families, UGT329, UGT330, UGT339, UGT341−UGT345, and UGT348−UGT350, with UGT344 containing the most (57). Ten <i<UGTs</i< (<i<UGT330A3</i<, <i<UGT339A2</i<, <i<UGT341A6</i<, <i<UGT342B3</i<, <i<UGT343C3</i<, <i<UGT344D5</i<, <i<UGT344D8</i<, <i<UGT348A3</i<, <i<UGT349A3</i<, and <i<UGT350A3</i<) were highly expressed in <i<M. persicae nicotianae</i< compared to <i<M. persicae</i< sensu stricto. Knockdown of four UGTs (<i<UGT330A3</i<, <i<UGT344D5</i<, <i<UGT348A3</i<, and <i<UGT349A3</i<) significantly increased <i<M. persicae nicotianae</i< sensitivity to nicotine, suggesting that <i<UGT</i< expression in this subspecies may be associated with nicotine tolerance and thus host adaptation. This study reveals possible <i<UGTs</i< relevant to nicotine adaptation in tobacco-consuming <i<M. persicae nicotianae</i<, and the findings will facilitate further validation of the roles of these <i<UGTs</i< in nicotine tolerance.
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Characterization of UDP-Glucuronosyltransferases and the Potential Contribution to Nicotine Tolerance in <i<Myzus persicae</i<

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