A hepatoprotective <it<Lindera obtusiloba </it<extract suppresses growth and attenuates insulin like growth factor-1 receptor signaling and NF-kappaB activity in human liver cancer cell lines

<p<Abstract</p< <p<Background</p< <p<In traditional Chinese and Korean medicine, an aqueous extract derived from wood and bark of the Japanese spice bush <it<Lindera obtusiloba </it<(<it<L.obtusiloba</it<) is applied to treat inflammations and chronic liver diseases including hepatocellular carcinoma. We previously demonstrated anti-fibrotic effects of <it<L.obtusiloba </it<extract in hepatic stellate cells. Thus, we here consequently examine anti-neoplastic effects of <it<L.obtusiloba </it<extract on human hepatocellular carcinoma (HCC) cell lines and the signaling pathways involved.</p< <p<Methods</p< <p<Four human HCC cell lines representing diverse stages of differentiation were treated with <it<L.obtusiloba </it<extract, standardized according to its known suppressive effects on proliferation and TGF-β-expression. Beside measurement of proliferation, invasion and apoptosis, effects on signal transduction and NF-κB-activity were determined.</p< <p<Results</p< <p<<it<L.obtusiloba </it<extract inhibited proliferation and induced apoptosis in all HCC cell lines and provoked a reduced basal and IGF-1-induced activation of the IGF-1R signaling cascade and a reduced transcriptional NF-κB-activity, particularly in the poorly differentiated SK-Hep1 cells. Pointing to anti-angiogenic effects, <it<L.obtusiloba </it<extract attenuated the basal and IGF-1-induced expression of hypoxia inducible factor-1α, vascular endothelial growth factor, peroxisome proliferator-activated receptor-γ, cyclooxygenase-2 and inducible nitric oxide synthase.</p< <p<Conclusions</p< <p<The traditional application of the extract is confirmed by our experimental data. Due to its potential to inhibit critical receptor tyrosine kinases involved in HCC progression via the IGF-1 signaling pathway and NF-κB, the standardized <it<L.obtusiloba </it<extract should be further analysed for its active compounds and explored as (complementary) treatment option for HCC.</p< Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Stroh Thorsten [verfasserIn] Kim Ki [verfasserIn] Trowitzsch-Kienast Wolfram [verfasserIn] Seehofer Daniel [verfasserIn] Neumann Ulf [verfasserIn] Erben Ulrike [verfasserIn] Ruehl Martin [verfasserIn] Freise Christian [verfasserIn] Zeitz Martin [verfasserIn] Somasundaram Rajan [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2011

Übergeordnetes Werk:	In: BMC Complementary and Alternative Medicine - BMC, 2003, 11(2011), 1, p 39
Übergeordnetes Werk:	volume:11 ; year:2011 ; number:1, p 39

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1472-6882-11-39

Katalog-ID:	DOAJ03479297X

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allfields	10.1186/1472-6882-11-39 doi (DE-627)DOAJ03479297X (DE-599)DOAJ456f717177084ad0a5900f29186c176c DE-627 ger DE-627 rakwb eng RZ201-999 Stroh Thorsten verfasserin aut A hepatoprotective <it<Lindera obtusiloba </it<extract suppresses growth and attenuates insulin like growth factor-1 receptor signaling and NF-kappaB activity in human liver cancer cell lines 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<In traditional Chinese and Korean medicine, an aqueous extract derived from wood and bark of the Japanese spice bush <it<Lindera obtusiloba </it<(<it<L.obtusiloba</it<) is applied to treat inflammations and chronic liver diseases including hepatocellular carcinoma. We previously demonstrated anti-fibrotic effects of <it<L.obtusiloba </it<extract in hepatic stellate cells. Thus, we here consequently examine anti-neoplastic effects of <it<L.obtusiloba </it<extract on human hepatocellular carcinoma (HCC) cell lines and the signaling pathways involved.</p< <p<Methods</p< <p<Four human HCC cell lines representing diverse stages of differentiation were treated with <it<L.obtusiloba </it<extract, standardized according to its known suppressive effects on proliferation and TGF-β-expression. Beside measurement of proliferation, invasion and apoptosis, effects on signal transduction and NF-κB-activity were determined.</p< <p<Results</p< <p<<it<L.obtusiloba </it<extract inhibited proliferation and induced apoptosis in all HCC cell lines and provoked a reduced basal and IGF-1-induced activation of the IGF-1R signaling cascade and a reduced transcriptional NF-κB-activity, particularly in the poorly differentiated SK-Hep1 cells. Pointing to anti-angiogenic effects, <it<L.obtusiloba </it<extract attenuated the basal and IGF-1-induced expression of hypoxia inducible factor-1α, vascular endothelial growth factor, peroxisome proliferator-activated receptor-γ, cyclooxygenase-2 and inducible nitric oxide synthase.</p< <p<Conclusions</p< <p<The traditional application of the extract is confirmed by our experimental data. Due to its potential to inhibit critical receptor tyrosine kinases involved in HCC progression via the IGF-1 signaling pathway and NF-κB, the standardized <it<L.obtusiloba </it<extract should be further analysed for its active compounds and explored as (complementary) treatment option for HCC.</p< Other systems of medicine Kim Ki verfasserin aut Trowitzsch-Kienast Wolfram verfasserin aut Seehofer Daniel verfasserin aut Neumann Ulf verfasserin aut Erben Ulrike verfasserin aut Ruehl Martin verfasserin aut Freise Christian verfasserin aut Zeitz Martin verfasserin aut Somasundaram Rajan verfasserin aut In BMC Complementary and Alternative Medicine BMC, 2003 11(2011), 1, p 39 (DE-627)331018713 (DE-600)2050429-9 14726882 nnns volume:11 year:2011 number:1, p 39 https://doi.org/10.1186/1472-6882-11-39 kostenfrei https://doaj.org/article/456f717177084ad0a5900f29186c176c kostenfrei http://www.biomedcentral.com/1472-6882/11/39 kostenfrei https://doaj.org/toc/1472-6882 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1, p 39
spelling	10.1186/1472-6882-11-39 doi (DE-627)DOAJ03479297X (DE-599)DOAJ456f717177084ad0a5900f29186c176c DE-627 ger DE-627 rakwb eng RZ201-999 Stroh Thorsten verfasserin aut A hepatoprotective <it<Lindera obtusiloba </it<extract suppresses growth and attenuates insulin like growth factor-1 receptor signaling and NF-kappaB activity in human liver cancer cell lines 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<In traditional Chinese and Korean medicine, an aqueous extract derived from wood and bark of the Japanese spice bush <it<Lindera obtusiloba </it<(<it<L.obtusiloba</it<) is applied to treat inflammations and chronic liver diseases including hepatocellular carcinoma. We previously demonstrated anti-fibrotic effects of <it<L.obtusiloba </it<extract in hepatic stellate cells. Thus, we here consequently examine anti-neoplastic effects of <it<L.obtusiloba </it<extract on human hepatocellular carcinoma (HCC) cell lines and the signaling pathways involved.</p< <p<Methods</p< <p<Four human HCC cell lines representing diverse stages of differentiation were treated with <it<L.obtusiloba </it<extract, standardized according to its known suppressive effects on proliferation and TGF-β-expression. Beside measurement of proliferation, invasion and apoptosis, effects on signal transduction and NF-κB-activity were determined.</p< <p<Results</p< <p<<it<L.obtusiloba </it<extract inhibited proliferation and induced apoptosis in all HCC cell lines and provoked a reduced basal and IGF-1-induced activation of the IGF-1R signaling cascade and a reduced transcriptional NF-κB-activity, particularly in the poorly differentiated SK-Hep1 cells. Pointing to anti-angiogenic effects, <it<L.obtusiloba </it<extract attenuated the basal and IGF-1-induced expression of hypoxia inducible factor-1α, vascular endothelial growth factor, peroxisome proliferator-activated receptor-γ, cyclooxygenase-2 and inducible nitric oxide synthase.</p< <p<Conclusions</p< <p<The traditional application of the extract is confirmed by our experimental data. Due to its potential to inhibit critical receptor tyrosine kinases involved in HCC progression via the IGF-1 signaling pathway and NF-κB, the standardized <it<L.obtusiloba </it<extract should be further analysed for its active compounds and explored as (complementary) treatment option for HCC.</p< Other systems of medicine Kim Ki verfasserin aut Trowitzsch-Kienast Wolfram verfasserin aut Seehofer Daniel verfasserin aut Neumann Ulf verfasserin aut Erben Ulrike verfasserin aut Ruehl Martin verfasserin aut Freise Christian verfasserin aut Zeitz Martin verfasserin aut Somasundaram Rajan verfasserin aut In BMC Complementary and Alternative Medicine BMC, 2003 11(2011), 1, p 39 (DE-627)331018713 (DE-600)2050429-9 14726882 nnns volume:11 year:2011 number:1, p 39 https://doi.org/10.1186/1472-6882-11-39 kostenfrei https://doaj.org/article/456f717177084ad0a5900f29186c176c kostenfrei http://www.biomedcentral.com/1472-6882/11/39 kostenfrei https://doaj.org/toc/1472-6882 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1, p 39
allfields_unstemmed	10.1186/1472-6882-11-39 doi (DE-627)DOAJ03479297X (DE-599)DOAJ456f717177084ad0a5900f29186c176c DE-627 ger DE-627 rakwb eng RZ201-999 Stroh Thorsten verfasserin aut A hepatoprotective <it<Lindera obtusiloba </it<extract suppresses growth and attenuates insulin like growth factor-1 receptor signaling and NF-kappaB activity in human liver cancer cell lines 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<In traditional Chinese and Korean medicine, an aqueous extract derived from wood and bark of the Japanese spice bush <it<Lindera obtusiloba </it<(<it<L.obtusiloba</it<) is applied to treat inflammations and chronic liver diseases including hepatocellular carcinoma. We previously demonstrated anti-fibrotic effects of <it<L.obtusiloba </it<extract in hepatic stellate cells. Thus, we here consequently examine anti-neoplastic effects of <it<L.obtusiloba </it<extract on human hepatocellular carcinoma (HCC) cell lines and the signaling pathways involved.</p< <p<Methods</p< <p<Four human HCC cell lines representing diverse stages of differentiation were treated with <it<L.obtusiloba </it<extract, standardized according to its known suppressive effects on proliferation and TGF-β-expression. Beside measurement of proliferation, invasion and apoptosis, effects on signal transduction and NF-κB-activity were determined.</p< <p<Results</p< <p<<it<L.obtusiloba </it<extract inhibited proliferation and induced apoptosis in all HCC cell lines and provoked a reduced basal and IGF-1-induced activation of the IGF-1R signaling cascade and a reduced transcriptional NF-κB-activity, particularly in the poorly differentiated SK-Hep1 cells. Pointing to anti-angiogenic effects, <it<L.obtusiloba </it<extract attenuated the basal and IGF-1-induced expression of hypoxia inducible factor-1α, vascular endothelial growth factor, peroxisome proliferator-activated receptor-γ, cyclooxygenase-2 and inducible nitric oxide synthase.</p< <p<Conclusions</p< <p<The traditional application of the extract is confirmed by our experimental data. Due to its potential to inhibit critical receptor tyrosine kinases involved in HCC progression via the IGF-1 signaling pathway and NF-κB, the standardized <it<L.obtusiloba </it<extract should be further analysed for its active compounds and explored as (complementary) treatment option for HCC.</p< Other systems of medicine Kim Ki verfasserin aut Trowitzsch-Kienast Wolfram verfasserin aut Seehofer Daniel verfasserin aut Neumann Ulf verfasserin aut Erben Ulrike verfasserin aut Ruehl Martin verfasserin aut Freise Christian verfasserin aut Zeitz Martin verfasserin aut Somasundaram Rajan verfasserin aut In BMC Complementary and Alternative Medicine BMC, 2003 11(2011), 1, p 39 (DE-627)331018713 (DE-600)2050429-9 14726882 nnns volume:11 year:2011 number:1, p 39 https://doi.org/10.1186/1472-6882-11-39 kostenfrei https://doaj.org/article/456f717177084ad0a5900f29186c176c kostenfrei http://www.biomedcentral.com/1472-6882/11/39 kostenfrei https://doaj.org/toc/1472-6882 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1, p 39
allfieldsGer	10.1186/1472-6882-11-39 doi (DE-627)DOAJ03479297X (DE-599)DOAJ456f717177084ad0a5900f29186c176c DE-627 ger DE-627 rakwb eng RZ201-999 Stroh Thorsten verfasserin aut A hepatoprotective <it<Lindera obtusiloba </it<extract suppresses growth and attenuates insulin like growth factor-1 receptor signaling and NF-kappaB activity in human liver cancer cell lines 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<In traditional Chinese and Korean medicine, an aqueous extract derived from wood and bark of the Japanese spice bush <it<Lindera obtusiloba </it<(<it<L.obtusiloba</it<) is applied to treat inflammations and chronic liver diseases including hepatocellular carcinoma. We previously demonstrated anti-fibrotic effects of <it<L.obtusiloba </it<extract in hepatic stellate cells. Thus, we here consequently examine anti-neoplastic effects of <it<L.obtusiloba </it<extract on human hepatocellular carcinoma (HCC) cell lines and the signaling pathways involved.</p< <p<Methods</p< <p<Four human HCC cell lines representing diverse stages of differentiation were treated with <it<L.obtusiloba </it<extract, standardized according to its known suppressive effects on proliferation and TGF-β-expression. Beside measurement of proliferation, invasion and apoptosis, effects on signal transduction and NF-κB-activity were determined.</p< <p<Results</p< <p<<it<L.obtusiloba </it<extract inhibited proliferation and induced apoptosis in all HCC cell lines and provoked a reduced basal and IGF-1-induced activation of the IGF-1R signaling cascade and a reduced transcriptional NF-κB-activity, particularly in the poorly differentiated SK-Hep1 cells. Pointing to anti-angiogenic effects, <it<L.obtusiloba </it<extract attenuated the basal and IGF-1-induced expression of hypoxia inducible factor-1α, vascular endothelial growth factor, peroxisome proliferator-activated receptor-γ, cyclooxygenase-2 and inducible nitric oxide synthase.</p< <p<Conclusions</p< <p<The traditional application of the extract is confirmed by our experimental data. Due to its potential to inhibit critical receptor tyrosine kinases involved in HCC progression via the IGF-1 signaling pathway and NF-κB, the standardized <it<L.obtusiloba </it<extract should be further analysed for its active compounds and explored as (complementary) treatment option for HCC.</p< Other systems of medicine Kim Ki verfasserin aut Trowitzsch-Kienast Wolfram verfasserin aut Seehofer Daniel verfasserin aut Neumann Ulf verfasserin aut Erben Ulrike verfasserin aut Ruehl Martin verfasserin aut Freise Christian verfasserin aut Zeitz Martin verfasserin aut Somasundaram Rajan verfasserin aut In BMC Complementary and Alternative Medicine BMC, 2003 11(2011), 1, p 39 (DE-627)331018713 (DE-600)2050429-9 14726882 nnns volume:11 year:2011 number:1, p 39 https://doi.org/10.1186/1472-6882-11-39 kostenfrei https://doaj.org/article/456f717177084ad0a5900f29186c176c kostenfrei http://www.biomedcentral.com/1472-6882/11/39 kostenfrei https://doaj.org/toc/1472-6882 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1, p 39
allfieldsSound	10.1186/1472-6882-11-39 doi (DE-627)DOAJ03479297X (DE-599)DOAJ456f717177084ad0a5900f29186c176c DE-627 ger DE-627 rakwb eng RZ201-999 Stroh Thorsten verfasserin aut A hepatoprotective <it<Lindera obtusiloba </it<extract suppresses growth and attenuates insulin like growth factor-1 receptor signaling and NF-kappaB activity in human liver cancer cell lines 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<In traditional Chinese and Korean medicine, an aqueous extract derived from wood and bark of the Japanese spice bush <it<Lindera obtusiloba </it<(<it<L.obtusiloba</it<) is applied to treat inflammations and chronic liver diseases including hepatocellular carcinoma. We previously demonstrated anti-fibrotic effects of <it<L.obtusiloba </it<extract in hepatic stellate cells. Thus, we here consequently examine anti-neoplastic effects of <it<L.obtusiloba </it<extract on human hepatocellular carcinoma (HCC) cell lines and the signaling pathways involved.</p< <p<Methods</p< <p<Four human HCC cell lines representing diverse stages of differentiation were treated with <it<L.obtusiloba </it<extract, standardized according to its known suppressive effects on proliferation and TGF-β-expression. Beside measurement of proliferation, invasion and apoptosis, effects on signal transduction and NF-κB-activity were determined.</p< <p<Results</p< <p<<it<L.obtusiloba </it<extract inhibited proliferation and induced apoptosis in all HCC cell lines and provoked a reduced basal and IGF-1-induced activation of the IGF-1R signaling cascade and a reduced transcriptional NF-κB-activity, particularly in the poorly differentiated SK-Hep1 cells. Pointing to anti-angiogenic effects, <it<L.obtusiloba </it<extract attenuated the basal and IGF-1-induced expression of hypoxia inducible factor-1α, vascular endothelial growth factor, peroxisome proliferator-activated receptor-γ, cyclooxygenase-2 and inducible nitric oxide synthase.</p< <p<Conclusions</p< <p<The traditional application of the extract is confirmed by our experimental data. Due to its potential to inhibit critical receptor tyrosine kinases involved in HCC progression via the IGF-1 signaling pathway and NF-κB, the standardized <it<L.obtusiloba </it<extract should be further analysed for its active compounds and explored as (complementary) treatment option for HCC.</p< Other systems of medicine Kim Ki verfasserin aut Trowitzsch-Kienast Wolfram verfasserin aut Seehofer Daniel verfasserin aut Neumann Ulf verfasserin aut Erben Ulrike verfasserin aut Ruehl Martin verfasserin aut Freise Christian verfasserin aut Zeitz Martin verfasserin aut Somasundaram Rajan verfasserin aut In BMC Complementary and Alternative Medicine BMC, 2003 11(2011), 1, p 39 (DE-627)331018713 (DE-600)2050429-9 14726882 nnns volume:11 year:2011 number:1, p 39 https://doi.org/10.1186/1472-6882-11-39 kostenfrei https://doaj.org/article/456f717177084ad0a5900f29186c176c kostenfrei http://www.biomedcentral.com/1472-6882/11/39 kostenfrei https://doaj.org/toc/1472-6882 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1, p 39
language	English
source	In BMC Complementary and Alternative Medicine 11(2011), 1, p 39 volume:11 year:2011 number:1, p 39
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topic_title	RZ201-999 A hepatoprotective <it<Lindera obtusiloba </it<extract suppresses growth and attenuates insulin like growth factor-1 receptor signaling and NF-kappaB activity in human liver cancer cell lines
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title	A hepatoprotective <it<Lindera obtusiloba </it<extract suppresses growth and attenuates insulin like growth factor-1 receptor signaling and NF-kappaB activity in human liver cancer cell lines
ctrlnum	(DE-627)DOAJ03479297X (DE-599)DOAJ456f717177084ad0a5900f29186c176c
title_full	A hepatoprotective <it<Lindera obtusiloba </it<extract suppresses growth and attenuates insulin like growth factor-1 receptor signaling and NF-kappaB activity in human liver cancer cell lines
author_sort	Stroh Thorsten
journal	BMC Complementary and Alternative Medicine
journalStr	BMC Complementary and Alternative Medicine
callnumber-first-code	R
lang_code	eng
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publishDateSort	2011
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author_browse	Stroh Thorsten Kim Ki Trowitzsch-Kienast Wolfram Seehofer Daniel Neumann Ulf Erben Ulrike Ruehl Martin Freise Christian Zeitz Martin Somasundaram Rajan
container_volume	11
class	RZ201-999
format_se	Elektronische Aufsätze
author-letter	Stroh Thorsten
doi_str_mv	10.1186/1472-6882-11-39
author2-role	verfasserin
title_sort	hepatoprotective <it<lindera obtusiloba </it<extract suppresses growth and attenuates insulin like growth factor-1 receptor signaling and nf-kappab activity in human liver cancer cell lines
callnumber	RZ201-999
title_auth	A hepatoprotective <it<Lindera obtusiloba </it<extract suppresses growth and attenuates insulin like growth factor-1 receptor signaling and NF-kappaB activity in human liver cancer cell lines
abstract	<p<Abstract</p< <p<Background</p< <p<In traditional Chinese and Korean medicine, an aqueous extract derived from wood and bark of the Japanese spice bush <it<Lindera obtusiloba </it<(<it<L.obtusiloba</it<) is applied to treat inflammations and chronic liver diseases including hepatocellular carcinoma. We previously demonstrated anti-fibrotic effects of <it<L.obtusiloba </it<extract in hepatic stellate cells. Thus, we here consequently examine anti-neoplastic effects of <it<L.obtusiloba </it<extract on human hepatocellular carcinoma (HCC) cell lines and the signaling pathways involved.</p< <p<Methods</p< <p<Four human HCC cell lines representing diverse stages of differentiation were treated with <it<L.obtusiloba </it<extract, standardized according to its known suppressive effects on proliferation and TGF-β-expression. Beside measurement of proliferation, invasion and apoptosis, effects on signal transduction and NF-κB-activity were determined.</p< <p<Results</p< <p<<it<L.obtusiloba </it<extract inhibited proliferation and induced apoptosis in all HCC cell lines and provoked a reduced basal and IGF-1-induced activation of the IGF-1R signaling cascade and a reduced transcriptional NF-κB-activity, particularly in the poorly differentiated SK-Hep1 cells. Pointing to anti-angiogenic effects, <it<L.obtusiloba </it<extract attenuated the basal and IGF-1-induced expression of hypoxia inducible factor-1α, vascular endothelial growth factor, peroxisome proliferator-activated receptor-γ, cyclooxygenase-2 and inducible nitric oxide synthase.</p< <p<Conclusions</p< <p<The traditional application of the extract is confirmed by our experimental data. Due to its potential to inhibit critical receptor tyrosine kinases involved in HCC progression via the IGF-1 signaling pathway and NF-κB, the standardized <it<L.obtusiloba </it<extract should be further analysed for its active compounds and explored as (complementary) treatment option for HCC.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<In traditional Chinese and Korean medicine, an aqueous extract derived from wood and bark of the Japanese spice bush <it<Lindera obtusiloba </it<(<it<L.obtusiloba</it<) is applied to treat inflammations and chronic liver diseases including hepatocellular carcinoma. We previously demonstrated anti-fibrotic effects of <it<L.obtusiloba </it<extract in hepatic stellate cells. Thus, we here consequently examine anti-neoplastic effects of <it<L.obtusiloba </it<extract on human hepatocellular carcinoma (HCC) cell lines and the signaling pathways involved.</p< <p<Methods</p< <p<Four human HCC cell lines representing diverse stages of differentiation were treated with <it<L.obtusiloba </it<extract, standardized according to its known suppressive effects on proliferation and TGF-β-expression. Beside measurement of proliferation, invasion and apoptosis, effects on signal transduction and NF-κB-activity were determined.</p< <p<Results</p< <p<<it<L.obtusiloba </it<extract inhibited proliferation and induced apoptosis in all HCC cell lines and provoked a reduced basal and IGF-1-induced activation of the IGF-1R signaling cascade and a reduced transcriptional NF-κB-activity, particularly in the poorly differentiated SK-Hep1 cells. Pointing to anti-angiogenic effects, <it<L.obtusiloba </it<extract attenuated the basal and IGF-1-induced expression of hypoxia inducible factor-1α, vascular endothelial growth factor, peroxisome proliferator-activated receptor-γ, cyclooxygenase-2 and inducible nitric oxide synthase.</p< <p<Conclusions</p< <p<The traditional application of the extract is confirmed by our experimental data. Due to its potential to inhibit critical receptor tyrosine kinases involved in HCC progression via the IGF-1 signaling pathway and NF-κB, the standardized <it<L.obtusiloba </it<extract should be further analysed for its active compounds and explored as (complementary) treatment option for HCC.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<In traditional Chinese and Korean medicine, an aqueous extract derived from wood and bark of the Japanese spice bush <it<Lindera obtusiloba </it<(<it<L.obtusiloba</it<) is applied to treat inflammations and chronic liver diseases including hepatocellular carcinoma. We previously demonstrated anti-fibrotic effects of <it<L.obtusiloba </it<extract in hepatic stellate cells. Thus, we here consequently examine anti-neoplastic effects of <it<L.obtusiloba </it<extract on human hepatocellular carcinoma (HCC) cell lines and the signaling pathways involved.</p< <p<Methods</p< <p<Four human HCC cell lines representing diverse stages of differentiation were treated with <it<L.obtusiloba </it<extract, standardized according to its known suppressive effects on proliferation and TGF-β-expression. Beside measurement of proliferation, invasion and apoptosis, effects on signal transduction and NF-κB-activity were determined.</p< <p<Results</p< <p<<it<L.obtusiloba </it<extract inhibited proliferation and induced apoptosis in all HCC cell lines and provoked a reduced basal and IGF-1-induced activation of the IGF-1R signaling cascade and a reduced transcriptional NF-κB-activity, particularly in the poorly differentiated SK-Hep1 cells. Pointing to anti-angiogenic effects, <it<L.obtusiloba </it<extract attenuated the basal and IGF-1-induced expression of hypoxia inducible factor-1α, vascular endothelial growth factor, peroxisome proliferator-activated receptor-γ, cyclooxygenase-2 and inducible nitric oxide synthase.</p< <p<Conclusions</p< <p<The traditional application of the extract is confirmed by our experimental data. Due to its potential to inhibit critical receptor tyrosine kinases involved in HCC progression via the IGF-1 signaling pathway and NF-κB, the standardized <it<L.obtusiloba </it<extract should be further analysed for its active compounds and explored as (complementary) treatment option for HCC.</p<
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container_issue	1, p 39
title_short	A hepatoprotective <it<Lindera obtusiloba </it<extract suppresses growth and attenuates insulin like growth factor-1 receptor signaling and NF-kappaB activity in human liver cancer cell lines
url	https://doi.org/10.1186/1472-6882-11-39 https://doaj.org/article/456f717177084ad0a5900f29186c176c http://www.biomedcentral.com/1472-6882/11/39 https://doaj.org/toc/1472-6882
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author2	Kim Ki Trowitzsch-Kienast Wolfram Seehofer Daniel Neumann Ulf Erben Ulrike Ruehl Martin Freise Christian Zeitz Martin Somasundaram Rajan
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up_date	2024-07-04T00:38:50.785Z
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A hepatoprotective <it<Lindera obtusiloba </it<extract suppresses growth and attenuates insulin like growth factor-1 receptor signaling and NF-kappaB activity in human liver cancer cell lines

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