Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells

Summary: In human pluripotent stem cells (hPSCs), traditional approaches for gene overexpression have low efficiency and are often laborious. Here, we provide a relatively simple protocol for gene overexpression with the Dox-inducible PiggyBac transposon system. We detail the steps for overexpressio...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Jiwen Yang [verfasserIn] Minjie Hu [verfasserIn] Yongyu Wang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Cell Biology Cell Differentiation Developmental biology Molecular Biology Stem Cells

Übergeordnetes Werk:	In: STAR Protocols - Elsevier, 2020, 3(2022), 2, Seite 101296-
Übergeordnetes Werk:	volume:3 ; year:2022 ; number:2 ; pages:101296-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.xpro.2022.101296

Katalog-ID:	DOAJ035001712

Internformat


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author_variant	j y jy m h mh y w yw
matchkey_str	article:26661667:2022----::rtcloidcbeigbcrnpsnytmoefceteevrxrsin
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allfields	10.1016/j.xpro.2022.101296 doi (DE-627)DOAJ035001712 (DE-599)DOAJbe434fc3d6eb43c492c7e557bf998716 DE-627 ger DE-627 rakwb eng Q1-390 Jiwen Yang verfasserin aut Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: In human pluripotent stem cells (hPSCs), traditional approaches for gene overexpression have low efficiency and are often laborious. Here, we provide a relatively simple protocol for gene overexpression with the Dox-inducible PiggyBac transposon system. We detail the steps for overexpression of FLI1 and/or YAP in H1 embryonic stem cells (H1 ESCs) as an example. Our protocol can be applied to any gene of interest in a variety of hPSCs.For complete details on the use and execution of this protocol, please refer to Quan et al. (2021). Cell Biology Cell Differentiation Developmental biology Molecular Biology Stem Cells Science (General) Minjie Hu verfasserin aut Yongyu Wang verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 2, Seite 101296- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:2 pages:101296- https://doi.org/10.1016/j.xpro.2022.101296 kostenfrei https://doaj.org/article/be434fc3d6eb43c492c7e557bf998716 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166722001769 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 2 101296-
spelling	10.1016/j.xpro.2022.101296 doi (DE-627)DOAJ035001712 (DE-599)DOAJbe434fc3d6eb43c492c7e557bf998716 DE-627 ger DE-627 rakwb eng Q1-390 Jiwen Yang verfasserin aut Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: In human pluripotent stem cells (hPSCs), traditional approaches for gene overexpression have low efficiency and are often laborious. Here, we provide a relatively simple protocol for gene overexpression with the Dox-inducible PiggyBac transposon system. We detail the steps for overexpression of FLI1 and/or YAP in H1 embryonic stem cells (H1 ESCs) as an example. Our protocol can be applied to any gene of interest in a variety of hPSCs.For complete details on the use and execution of this protocol, please refer to Quan et al. (2021). Cell Biology Cell Differentiation Developmental biology Molecular Biology Stem Cells Science (General) Minjie Hu verfasserin aut Yongyu Wang verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 2, Seite 101296- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:2 pages:101296- https://doi.org/10.1016/j.xpro.2022.101296 kostenfrei https://doaj.org/article/be434fc3d6eb43c492c7e557bf998716 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166722001769 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 2 101296-
allfields_unstemmed	10.1016/j.xpro.2022.101296 doi (DE-627)DOAJ035001712 (DE-599)DOAJbe434fc3d6eb43c492c7e557bf998716 DE-627 ger DE-627 rakwb eng Q1-390 Jiwen Yang verfasserin aut Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: In human pluripotent stem cells (hPSCs), traditional approaches for gene overexpression have low efficiency and are often laborious. Here, we provide a relatively simple protocol for gene overexpression with the Dox-inducible PiggyBac transposon system. We detail the steps for overexpression of FLI1 and/or YAP in H1 embryonic stem cells (H1 ESCs) as an example. Our protocol can be applied to any gene of interest in a variety of hPSCs.For complete details on the use and execution of this protocol, please refer to Quan et al. (2021). Cell Biology Cell Differentiation Developmental biology Molecular Biology Stem Cells Science (General) Minjie Hu verfasserin aut Yongyu Wang verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 2, Seite 101296- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:2 pages:101296- https://doi.org/10.1016/j.xpro.2022.101296 kostenfrei https://doaj.org/article/be434fc3d6eb43c492c7e557bf998716 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166722001769 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 2 101296-
allfieldsGer	10.1016/j.xpro.2022.101296 doi (DE-627)DOAJ035001712 (DE-599)DOAJbe434fc3d6eb43c492c7e557bf998716 DE-627 ger DE-627 rakwb eng Q1-390 Jiwen Yang verfasserin aut Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: In human pluripotent stem cells (hPSCs), traditional approaches for gene overexpression have low efficiency and are often laborious. Here, we provide a relatively simple protocol for gene overexpression with the Dox-inducible PiggyBac transposon system. We detail the steps for overexpression of FLI1 and/or YAP in H1 embryonic stem cells (H1 ESCs) as an example. Our protocol can be applied to any gene of interest in a variety of hPSCs.For complete details on the use and execution of this protocol, please refer to Quan et al. (2021). Cell Biology Cell Differentiation Developmental biology Molecular Biology Stem Cells Science (General) Minjie Hu verfasserin aut Yongyu Wang verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 2, Seite 101296- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:2 pages:101296- https://doi.org/10.1016/j.xpro.2022.101296 kostenfrei https://doaj.org/article/be434fc3d6eb43c492c7e557bf998716 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166722001769 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 2 101296-
allfieldsSound	10.1016/j.xpro.2022.101296 doi (DE-627)DOAJ035001712 (DE-599)DOAJbe434fc3d6eb43c492c7e557bf998716 DE-627 ger DE-627 rakwb eng Q1-390 Jiwen Yang verfasserin aut Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: In human pluripotent stem cells (hPSCs), traditional approaches for gene overexpression have low efficiency and are often laborious. Here, we provide a relatively simple protocol for gene overexpression with the Dox-inducible PiggyBac transposon system. We detail the steps for overexpression of FLI1 and/or YAP in H1 embryonic stem cells (H1 ESCs) as an example. Our protocol can be applied to any gene of interest in a variety of hPSCs.For complete details on the use and execution of this protocol, please refer to Quan et al. (2021). Cell Biology Cell Differentiation Developmental biology Molecular Biology Stem Cells Science (General) Minjie Hu verfasserin aut Yongyu Wang verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 2, Seite 101296- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:2 pages:101296- https://doi.org/10.1016/j.xpro.2022.101296 kostenfrei https://doaj.org/article/be434fc3d6eb43c492c7e557bf998716 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166722001769 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 2 101296-
language	English
source	In STAR Protocols 3(2022), 2, Seite 101296- volume:3 year:2022 number:2 pages:101296-
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authorswithroles_txt_mv	Jiwen Yang @@aut@@ Minjie Hu @@aut@@ Yongyu Wang @@aut@@
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callnumber-first	Q - Science
author	Jiwen Yang
spellingShingle	Jiwen Yang misc Q1-390 misc Cell Biology misc Cell Differentiation misc Developmental biology misc Molecular Biology misc Stem Cells misc Science (General) Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells
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topic_title	Q1-390 Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells Cell Biology Cell Differentiation Developmental biology Molecular Biology Stem Cells
topic	misc Q1-390 misc Cell Biology misc Cell Differentiation misc Developmental biology misc Molecular Biology misc Stem Cells misc Science (General)
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title	Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells
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title_full	Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells
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title_sort	protocol for inducible piggybac transposon system for efficient gene overexpression in human pluripotent stem cells
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title_auth	Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells
abstract	Summary: In human pluripotent stem cells (hPSCs), traditional approaches for gene overexpression have low efficiency and are often laborious. Here, we provide a relatively simple protocol for gene overexpression with the Dox-inducible PiggyBac transposon system. We detail the steps for overexpression of FLI1 and/or YAP in H1 embryonic stem cells (H1 ESCs) as an example. Our protocol can be applied to any gene of interest in a variety of hPSCs.For complete details on the use and execution of this protocol, please refer to Quan et al. (2021).
abstractGer	Summary: In human pluripotent stem cells (hPSCs), traditional approaches for gene overexpression have low efficiency and are often laborious. Here, we provide a relatively simple protocol for gene overexpression with the Dox-inducible PiggyBac transposon system. We detail the steps for overexpression of FLI1 and/or YAP in H1 embryonic stem cells (H1 ESCs) as an example. Our protocol can be applied to any gene of interest in a variety of hPSCs.For complete details on the use and execution of this protocol, please refer to Quan et al. (2021).
abstract_unstemmed	Summary: In human pluripotent stem cells (hPSCs), traditional approaches for gene overexpression have low efficiency and are often laborious. Here, we provide a relatively simple protocol for gene overexpression with the Dox-inducible PiggyBac transposon system. We detail the steps for overexpression of FLI1 and/or YAP in H1 embryonic stem cells (H1 ESCs) as an example. Our protocol can be applied to any gene of interest in a variety of hPSCs.For complete details on the use and execution of this protocol, please refer to Quan et al. (2021).
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title_short	Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells
url	https://doi.org/10.1016/j.xpro.2022.101296 https://doaj.org/article/be434fc3d6eb43c492c7e557bf998716 http://www.sciencedirect.com/science/article/pii/S2666166722001769 https://doaj.org/toc/2666-1667
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Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells

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