Expression of hypoxia inducible factor-1α (HIF-1α) gene and apoptosis in the heart induced by systemic hypoxia

Aim This study explored the expression of HIF-1α in hypoxic cardiac muscle in mice, and observed the evidence of apoptosis in hypoxia induced cardiomyocyte. Methods Male Sprague-Dawley rats, were randomized into 7 groups (n = 4 per group): control normoxia group that was exposed to atmospheric oxyg...
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Autor*in:	Siufui Hendrawan [verfasserIn] Sri W.A. Jusman [verfasserIn] Frans Ferdinal [verfasserIn] Ani R. Prijanti [verfasserIn] Septelia I. Wanandi [verfasserIn] Mohamad Sadikin [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2009

Übergeordnetes Werk:	In: Medical Journal of Indonesia - Faculty of Medicine Universitas Indonesia, 2013, 18(2009), 2
Übergeordnetes Werk:	volume:18 ; year:2009 ; number:2

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.13181/mji.v18i2.344

Katalog-ID:	DOAJ035060638

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520			\|a Aim This study explored the expression of HIF-1α in hypoxic cardiac muscle in mice, and observed the evidence of apoptosis in hypoxia induced cardiomyocyte. Methods Male Sprague-Dawley rats, were randomized into 7 groups (n = 4 per group): control normoxia group that was exposed to atmospheric oxygen and hypoxia groups that were housed in hypoxic chambers (O2 level 8%) for 1, 3, 7, 14, 21, and 28 days respectively. Animals were sacrificed, hearts were rapidly excised, total RNA was extracted with an mRNA isolation kit and the expression of HIF-1α mRNA was then detected by real-time RT-PCR. Apoptosis was assessed by TUNEL method. Results For rat in hypoxia group, the expression of HIF-1α mRNA in cardiac myocytes was clearly up-regulated compared to the control normoxia group. Further, HIF-1α expression level elevated gradually and reached a peak at 21 days of hypoxia. No cell labeled by the TUNEL method was detected in the control group. Compared with the control group, the apoptotic index was significantly increased in the hypoxia group (P < 0.05). There was no significant correlation between the elevation of HIF-1α mRNA and the elevation of apoptotic index. Conclusion Systemic chronic hypoxia caused the elevation of HIF-1α mRNA and apoptosis in cardiac myocytes. (Med J Indones 2009; 18: 97-101) Keywords: TUNEL, RT-PCR, mRNA, apoptotic index
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allfields	10.13181/mji.v18i2.344 doi (DE-627)DOAJ035060638 (DE-599)DOAJ4e152e63a5274e988112f3b47c765214 DE-627 ger DE-627 rakwb eng R5-920 Siufui Hendrawan verfasserin aut Expression of hypoxia inducible factor-1α (HIF-1α) gene and apoptosis in the heart induced by systemic hypoxia 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aim This study explored the expression of HIF-1α in hypoxic cardiac muscle in mice, and observed the evidence of apoptosis in hypoxia induced cardiomyocyte. Methods Male Sprague-Dawley rats, were randomized into 7 groups (n = 4 per group): control normoxia group that was exposed to atmospheric oxygen and hypoxia groups that were housed in hypoxic chambers (O2 level 8%) for 1, 3, 7, 14, 21, and 28 days respectively. Animals were sacrificed, hearts were rapidly excised, total RNA was extracted with an mRNA isolation kit and the expression of HIF-1α mRNA was then detected by real-time RT-PCR. Apoptosis was assessed by TUNEL method. Results For rat in hypoxia group, the expression of HIF-1α mRNA in cardiac myocytes was clearly up-regulated compared to the control normoxia group. Further, HIF-1α expression level elevated gradually and reached a peak at 21 days of hypoxia. No cell labeled by the TUNEL method was detected in the control group. Compared with the control group, the apoptotic index was significantly increased in the hypoxia group (P < 0.05). There was no significant correlation between the elevation of HIF-1α mRNA and the elevation of apoptotic index. Conclusion Systemic chronic hypoxia caused the elevation of HIF-1α mRNA and apoptosis in cardiac myocytes. (Med J Indones 2009; 18: 97-101) Keywords: TUNEL, RT-PCR, mRNA, apoptotic index Medicine (General) Sri W.A. Jusman verfasserin aut Frans Ferdinal verfasserin aut Ani R. Prijanti verfasserin aut Septelia I. Wanandi verfasserin aut Mohamad Sadikin verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 18(2009), 2 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:18 year:2009 number:2 https://doi.org/10.13181/mji.v18i2.344 kostenfrei https://doaj.org/article/4e152e63a5274e988112f3b47c765214 kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/344 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 18 2009 2
spelling	10.13181/mji.v18i2.344 doi (DE-627)DOAJ035060638 (DE-599)DOAJ4e152e63a5274e988112f3b47c765214 DE-627 ger DE-627 rakwb eng R5-920 Siufui Hendrawan verfasserin aut Expression of hypoxia inducible factor-1α (HIF-1α) gene and apoptosis in the heart induced by systemic hypoxia 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aim This study explored the expression of HIF-1α in hypoxic cardiac muscle in mice, and observed the evidence of apoptosis in hypoxia induced cardiomyocyte. Methods Male Sprague-Dawley rats, were randomized into 7 groups (n = 4 per group): control normoxia group that was exposed to atmospheric oxygen and hypoxia groups that were housed in hypoxic chambers (O2 level 8%) for 1, 3, 7, 14, 21, and 28 days respectively. Animals were sacrificed, hearts were rapidly excised, total RNA was extracted with an mRNA isolation kit and the expression of HIF-1α mRNA was then detected by real-time RT-PCR. Apoptosis was assessed by TUNEL method. Results For rat in hypoxia group, the expression of HIF-1α mRNA in cardiac myocytes was clearly up-regulated compared to the control normoxia group. Further, HIF-1α expression level elevated gradually and reached a peak at 21 days of hypoxia. No cell labeled by the TUNEL method was detected in the control group. Compared with the control group, the apoptotic index was significantly increased in the hypoxia group (P < 0.05). There was no significant correlation between the elevation of HIF-1α mRNA and the elevation of apoptotic index. Conclusion Systemic chronic hypoxia caused the elevation of HIF-1α mRNA and apoptosis in cardiac myocytes. (Med J Indones 2009; 18: 97-101) Keywords: TUNEL, RT-PCR, mRNA, apoptotic index Medicine (General) Sri W.A. Jusman verfasserin aut Frans Ferdinal verfasserin aut Ani R. Prijanti verfasserin aut Septelia I. Wanandi verfasserin aut Mohamad Sadikin verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 18(2009), 2 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:18 year:2009 number:2 https://doi.org/10.13181/mji.v18i2.344 kostenfrei https://doaj.org/article/4e152e63a5274e988112f3b47c765214 kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/344 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 18 2009 2
allfields_unstemmed	10.13181/mji.v18i2.344 doi (DE-627)DOAJ035060638 (DE-599)DOAJ4e152e63a5274e988112f3b47c765214 DE-627 ger DE-627 rakwb eng R5-920 Siufui Hendrawan verfasserin aut Expression of hypoxia inducible factor-1α (HIF-1α) gene and apoptosis in the heart induced by systemic hypoxia 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aim This study explored the expression of HIF-1α in hypoxic cardiac muscle in mice, and observed the evidence of apoptosis in hypoxia induced cardiomyocyte. Methods Male Sprague-Dawley rats, were randomized into 7 groups (n = 4 per group): control normoxia group that was exposed to atmospheric oxygen and hypoxia groups that were housed in hypoxic chambers (O2 level 8%) for 1, 3, 7, 14, 21, and 28 days respectively. Animals were sacrificed, hearts were rapidly excised, total RNA was extracted with an mRNA isolation kit and the expression of HIF-1α mRNA was then detected by real-time RT-PCR. Apoptosis was assessed by TUNEL method. Results For rat in hypoxia group, the expression of HIF-1α mRNA in cardiac myocytes was clearly up-regulated compared to the control normoxia group. Further, HIF-1α expression level elevated gradually and reached a peak at 21 days of hypoxia. No cell labeled by the TUNEL method was detected in the control group. Compared with the control group, the apoptotic index was significantly increased in the hypoxia group (P < 0.05). There was no significant correlation between the elevation of HIF-1α mRNA and the elevation of apoptotic index. Conclusion Systemic chronic hypoxia caused the elevation of HIF-1α mRNA and apoptosis in cardiac myocytes. (Med J Indones 2009; 18: 97-101) Keywords: TUNEL, RT-PCR, mRNA, apoptotic index Medicine (General) Sri W.A. Jusman verfasserin aut Frans Ferdinal verfasserin aut Ani R. Prijanti verfasserin aut Septelia I. Wanandi verfasserin aut Mohamad Sadikin verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 18(2009), 2 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:18 year:2009 number:2 https://doi.org/10.13181/mji.v18i2.344 kostenfrei https://doaj.org/article/4e152e63a5274e988112f3b47c765214 kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/344 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 18 2009 2
allfieldsGer	10.13181/mji.v18i2.344 doi (DE-627)DOAJ035060638 (DE-599)DOAJ4e152e63a5274e988112f3b47c765214 DE-627 ger DE-627 rakwb eng R5-920 Siufui Hendrawan verfasserin aut Expression of hypoxia inducible factor-1α (HIF-1α) gene and apoptosis in the heart induced by systemic hypoxia 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aim This study explored the expression of HIF-1α in hypoxic cardiac muscle in mice, and observed the evidence of apoptosis in hypoxia induced cardiomyocyte. Methods Male Sprague-Dawley rats, were randomized into 7 groups (n = 4 per group): control normoxia group that was exposed to atmospheric oxygen and hypoxia groups that were housed in hypoxic chambers (O2 level 8%) for 1, 3, 7, 14, 21, and 28 days respectively. Animals were sacrificed, hearts were rapidly excised, total RNA was extracted with an mRNA isolation kit and the expression of HIF-1α mRNA was then detected by real-time RT-PCR. Apoptosis was assessed by TUNEL method. Results For rat in hypoxia group, the expression of HIF-1α mRNA in cardiac myocytes was clearly up-regulated compared to the control normoxia group. Further, HIF-1α expression level elevated gradually and reached a peak at 21 days of hypoxia. No cell labeled by the TUNEL method was detected in the control group. Compared with the control group, the apoptotic index was significantly increased in the hypoxia group (P < 0.05). There was no significant correlation between the elevation of HIF-1α mRNA and the elevation of apoptotic index. Conclusion Systemic chronic hypoxia caused the elevation of HIF-1α mRNA and apoptosis in cardiac myocytes. (Med J Indones 2009; 18: 97-101) Keywords: TUNEL, RT-PCR, mRNA, apoptotic index Medicine (General) Sri W.A. Jusman verfasserin aut Frans Ferdinal verfasserin aut Ani R. Prijanti verfasserin aut Septelia I. Wanandi verfasserin aut Mohamad Sadikin verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 18(2009), 2 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:18 year:2009 number:2 https://doi.org/10.13181/mji.v18i2.344 kostenfrei https://doaj.org/article/4e152e63a5274e988112f3b47c765214 kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/344 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 18 2009 2
allfieldsSound	10.13181/mji.v18i2.344 doi (DE-627)DOAJ035060638 (DE-599)DOAJ4e152e63a5274e988112f3b47c765214 DE-627 ger DE-627 rakwb eng R5-920 Siufui Hendrawan verfasserin aut Expression of hypoxia inducible factor-1α (HIF-1α) gene and apoptosis in the heart induced by systemic hypoxia 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aim This study explored the expression of HIF-1α in hypoxic cardiac muscle in mice, and observed the evidence of apoptosis in hypoxia induced cardiomyocyte. Methods Male Sprague-Dawley rats, were randomized into 7 groups (n = 4 per group): control normoxia group that was exposed to atmospheric oxygen and hypoxia groups that were housed in hypoxic chambers (O2 level 8%) for 1, 3, 7, 14, 21, and 28 days respectively. Animals were sacrificed, hearts were rapidly excised, total RNA was extracted with an mRNA isolation kit and the expression of HIF-1α mRNA was then detected by real-time RT-PCR. Apoptosis was assessed by TUNEL method. Results For rat in hypoxia group, the expression of HIF-1α mRNA in cardiac myocytes was clearly up-regulated compared to the control normoxia group. Further, HIF-1α expression level elevated gradually and reached a peak at 21 days of hypoxia. No cell labeled by the TUNEL method was detected in the control group. Compared with the control group, the apoptotic index was significantly increased in the hypoxia group (P < 0.05). There was no significant correlation between the elevation of HIF-1α mRNA and the elevation of apoptotic index. Conclusion Systemic chronic hypoxia caused the elevation of HIF-1α mRNA and apoptosis in cardiac myocytes. (Med J Indones 2009; 18: 97-101) Keywords: TUNEL, RT-PCR, mRNA, apoptotic index Medicine (General) Sri W.A. Jusman verfasserin aut Frans Ferdinal verfasserin aut Ani R. Prijanti verfasserin aut Septelia I. Wanandi verfasserin aut Mohamad Sadikin verfasserin aut In Medical Journal of Indonesia Faculty of Medicine Universitas Indonesia, 2013 18(2009), 2 (DE-627)747137862 (DE-600)2716886-4 22528083 nnns volume:18 year:2009 number:2 https://doi.org/10.13181/mji.v18i2.344 kostenfrei https://doaj.org/article/4e152e63a5274e988112f3b47c765214 kostenfrei http://mji.ui.ac.id/journal/index.php/mji/article/view/344 kostenfrei https://doaj.org/toc/0853-1773 Journal toc kostenfrei https://doaj.org/toc/2252-8083 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 18 2009 2
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callnumber-first	R - Medicine
author	Siufui Hendrawan
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topic_title	R5-920 Expression of hypoxia inducible factor-1α (HIF-1α) gene and apoptosis in the heart induced by systemic hypoxia
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title	Expression of hypoxia inducible factor-1α (HIF-1α) gene and apoptosis in the heart induced by systemic hypoxia
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title_full	Expression of hypoxia inducible factor-1α (HIF-1α) gene and apoptosis in the heart induced by systemic hypoxia
author_sort	Siufui Hendrawan
journal	Medical Journal of Indonesia
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author_browse	Siufui Hendrawan Sri W.A. Jusman Frans Ferdinal Ani R. Prijanti Septelia I. Wanandi Mohamad Sadikin
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doi_str_mv	10.13181/mji.v18i2.344
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title_sort	expression of hypoxia inducible factor-1α (hif-1α) gene and apoptosis in the heart induced by systemic hypoxia
callnumber	R5-920
title_auth	Expression of hypoxia inducible factor-1α (HIF-1α) gene and apoptosis in the heart induced by systemic hypoxia
abstract	Aim This study explored the expression of HIF-1α in hypoxic cardiac muscle in mice, and observed the evidence of apoptosis in hypoxia induced cardiomyocyte. Methods Male Sprague-Dawley rats, were randomized into 7 groups (n = 4 per group): control normoxia group that was exposed to atmospheric oxygen and hypoxia groups that were housed in hypoxic chambers (O2 level 8%) for 1, 3, 7, 14, 21, and 28 days respectively. Animals were sacrificed, hearts were rapidly excised, total RNA was extracted with an mRNA isolation kit and the expression of HIF-1α mRNA was then detected by real-time RT-PCR. Apoptosis was assessed by TUNEL method. Results For rat in hypoxia group, the expression of HIF-1α mRNA in cardiac myocytes was clearly up-regulated compared to the control normoxia group. Further, HIF-1α expression level elevated gradually and reached a peak at 21 days of hypoxia. No cell labeled by the TUNEL method was detected in the control group. Compared with the control group, the apoptotic index was significantly increased in the hypoxia group (P < 0.05). There was no significant correlation between the elevation of HIF-1α mRNA and the elevation of apoptotic index. Conclusion Systemic chronic hypoxia caused the elevation of HIF-1α mRNA and apoptosis in cardiac myocytes. (Med J Indones 2009; 18: 97-101) Keywords: TUNEL, RT-PCR, mRNA, apoptotic index
abstractGer	Aim This study explored the expression of HIF-1α in hypoxic cardiac muscle in mice, and observed the evidence of apoptosis in hypoxia induced cardiomyocyte. Methods Male Sprague-Dawley rats, were randomized into 7 groups (n = 4 per group): control normoxia group that was exposed to atmospheric oxygen and hypoxia groups that were housed in hypoxic chambers (O2 level 8%) for 1, 3, 7, 14, 21, and 28 days respectively. Animals were sacrificed, hearts were rapidly excised, total RNA was extracted with an mRNA isolation kit and the expression of HIF-1α mRNA was then detected by real-time RT-PCR. Apoptosis was assessed by TUNEL method. Results For rat in hypoxia group, the expression of HIF-1α mRNA in cardiac myocytes was clearly up-regulated compared to the control normoxia group. Further, HIF-1α expression level elevated gradually and reached a peak at 21 days of hypoxia. No cell labeled by the TUNEL method was detected in the control group. Compared with the control group, the apoptotic index was significantly increased in the hypoxia group (P < 0.05). There was no significant correlation between the elevation of HIF-1α mRNA and the elevation of apoptotic index. Conclusion Systemic chronic hypoxia caused the elevation of HIF-1α mRNA and apoptosis in cardiac myocytes. (Med J Indones 2009; 18: 97-101) Keywords: TUNEL, RT-PCR, mRNA, apoptotic index
abstract_unstemmed	Aim This study explored the expression of HIF-1α in hypoxic cardiac muscle in mice, and observed the evidence of apoptosis in hypoxia induced cardiomyocyte. Methods Male Sprague-Dawley rats, were randomized into 7 groups (n = 4 per group): control normoxia group that was exposed to atmospheric oxygen and hypoxia groups that were housed in hypoxic chambers (O2 level 8%) for 1, 3, 7, 14, 21, and 28 days respectively. Animals were sacrificed, hearts were rapidly excised, total RNA was extracted with an mRNA isolation kit and the expression of HIF-1α mRNA was then detected by real-time RT-PCR. Apoptosis was assessed by TUNEL method. Results For rat in hypoxia group, the expression of HIF-1α mRNA in cardiac myocytes was clearly up-regulated compared to the control normoxia group. Further, HIF-1α expression level elevated gradually and reached a peak at 21 days of hypoxia. No cell labeled by the TUNEL method was detected in the control group. Compared with the control group, the apoptotic index was significantly increased in the hypoxia group (P < 0.05). There was no significant correlation between the elevation of HIF-1α mRNA and the elevation of apoptotic index. Conclusion Systemic chronic hypoxia caused the elevation of HIF-1α mRNA and apoptosis in cardiac myocytes. (Med J Indones 2009; 18: 97-101) Keywords: TUNEL, RT-PCR, mRNA, apoptotic index
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title_short	Expression of hypoxia inducible factor-1α (HIF-1α) gene and apoptosis in the heart induced by systemic hypoxia
url	https://doi.org/10.13181/mji.v18i2.344 https://doaj.org/article/4e152e63a5274e988112f3b47c765214 http://mji.ui.ac.id/journal/index.php/mji/article/view/344 https://doaj.org/toc/0853-1773 https://doaj.org/toc/2252-8083
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author2	Sri W.A. Jusman Frans Ferdinal Ani R. Prijanti Septelia I. Wanandi Mohamad Sadikin
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up_date	2024-07-04T01:37:10.178Z
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