Evolutionary analysis of proline-directed phosphorylation sites in the mammalian growth cone identified using phosphoproteomics

Abstract The growth cone is essential for nerve growth and axon regeneration, which directly form and rearrange the neural network. Recently, to clarify the molecular signaling pathways in the growth cone that utilize protein phosphorylation, we performed a phosphoproteomics study of mammalian growt...
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Autor*in:	Michihiro Igarashi [verfasserIn] Shujiro Okuda [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Growth cone Phosphoproteomics Bioinformatics MAPK Evolution Vertebrates

Übergeordnetes Werk:	In: Molecular Brain - BMC, 2010, 12(2019), 1, Seite 3
Übergeordnetes Werk:	volume:12 ; year:2019 ; number:1 ; pages:3

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/s13041-019-0476-x

Katalog-ID:	DOAJ035180277

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520			\|a Abstract The growth cone is essential for nerve growth and axon regeneration, which directly form and rearrange the neural network. Recently, to clarify the molecular signaling pathways in the growth cone that utilize protein phosphorylation, we performed a phosphoproteomics study of mammalian growth cone membranes derived from the developing rodent brain and identified < 30,000 phosphopeptides from ~ 1200 proteins. We found that the phosphorylation sites were highly proline directed and primarily mitogen-activated protein kinase (MAPK) dependent, due to particular activation of c-jun N-terminal protein kinase (JNK), a member of the MAPK family. Because the MAPK/JNK pathway is also involved in axon regeneration of invertebrate model organisms such Caenorhabditis elegans and Drosophila, we performed evolutionary bioinformatics analysis of the mammalian growth cone phosphorylation sites. Although these sites were generally conserved within vertebrates, they were not necessarily conserved in these invertebrate model organisms. In particular, high-frequency phosphorylation sites (< 20 times) were less conserved than low-frequency sites. Taken together, the mammalian growth cones contain a large number of vertebrate-specific phosphorylation sites and stronger dependence upon MAPK/JNK than C. elegans or Drosophila. We conclude that axon growth/regeneration likely involves many vertebrate-specific phosphorylation sites.
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allfields	10.1186/s13041-019-0476-x doi (DE-627)DOAJ035180277 (DE-599)DOAJ8fe9de2ee9f048829bf10ee546732c30 DE-627 ger DE-627 rakwb eng RC346-429 Michihiro Igarashi verfasserin aut Evolutionary analysis of proline-directed phosphorylation sites in the mammalian growth cone identified using phosphoproteomics 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The growth cone is essential for nerve growth and axon regeneration, which directly form and rearrange the neural network. Recently, to clarify the molecular signaling pathways in the growth cone that utilize protein phosphorylation, we performed a phosphoproteomics study of mammalian growth cone membranes derived from the developing rodent brain and identified < 30,000 phosphopeptides from ~ 1200 proteins. We found that the phosphorylation sites were highly proline directed and primarily mitogen-activated protein kinase (MAPK) dependent, due to particular activation of c-jun N-terminal protein kinase (JNK), a member of the MAPK family. Because the MAPK/JNK pathway is also involved in axon regeneration of invertebrate model organisms such Caenorhabditis elegans and Drosophila, we performed evolutionary bioinformatics analysis of the mammalian growth cone phosphorylation sites. Although these sites were generally conserved within vertebrates, they were not necessarily conserved in these invertebrate model organisms. In particular, high-frequency phosphorylation sites (< 20 times) were less conserved than low-frequency sites. Taken together, the mammalian growth cones contain a large number of vertebrate-specific phosphorylation sites and stronger dependence upon MAPK/JNK than C. elegans or Drosophila. We conclude that axon growth/regeneration likely involves many vertebrate-specific phosphorylation sites. Growth cone Phosphoproteomics Bioinformatics MAPK Evolution Vertebrates Neurology. Diseases of the nervous system Shujiro Okuda verfasserin aut In Molecular Brain BMC, 2010 12(2019), 1, Seite 3 (DE-627)571612873 (DE-600)2436057-0 17566606 nnns volume:12 year:2019 number:1 pages:3 https://doi.org/10.1186/s13041-019-0476-x kostenfrei https://doaj.org/article/8fe9de2ee9f048829bf10ee546732c30 kostenfrei http://link.springer.com/article/10.1186/s13041-019-0476-x kostenfrei https://doaj.org/toc/1756-6606 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2019 1 3
spelling	10.1186/s13041-019-0476-x doi (DE-627)DOAJ035180277 (DE-599)DOAJ8fe9de2ee9f048829bf10ee546732c30 DE-627 ger DE-627 rakwb eng RC346-429 Michihiro Igarashi verfasserin aut Evolutionary analysis of proline-directed phosphorylation sites in the mammalian growth cone identified using phosphoproteomics 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The growth cone is essential for nerve growth and axon regeneration, which directly form and rearrange the neural network. Recently, to clarify the molecular signaling pathways in the growth cone that utilize protein phosphorylation, we performed a phosphoproteomics study of mammalian growth cone membranes derived from the developing rodent brain and identified < 30,000 phosphopeptides from ~ 1200 proteins. We found that the phosphorylation sites were highly proline directed and primarily mitogen-activated protein kinase (MAPK) dependent, due to particular activation of c-jun N-terminal protein kinase (JNK), a member of the MAPK family. Because the MAPK/JNK pathway is also involved in axon regeneration of invertebrate model organisms such Caenorhabditis elegans and Drosophila, we performed evolutionary bioinformatics analysis of the mammalian growth cone phosphorylation sites. Although these sites were generally conserved within vertebrates, they were not necessarily conserved in these invertebrate model organisms. In particular, high-frequency phosphorylation sites (< 20 times) were less conserved than low-frequency sites. Taken together, the mammalian growth cones contain a large number of vertebrate-specific phosphorylation sites and stronger dependence upon MAPK/JNK than C. elegans or Drosophila. We conclude that axon growth/regeneration likely involves many vertebrate-specific phosphorylation sites. Growth cone Phosphoproteomics Bioinformatics MAPK Evolution Vertebrates Neurology. Diseases of the nervous system Shujiro Okuda verfasserin aut In Molecular Brain BMC, 2010 12(2019), 1, Seite 3 (DE-627)571612873 (DE-600)2436057-0 17566606 nnns volume:12 year:2019 number:1 pages:3 https://doi.org/10.1186/s13041-019-0476-x kostenfrei https://doaj.org/article/8fe9de2ee9f048829bf10ee546732c30 kostenfrei http://link.springer.com/article/10.1186/s13041-019-0476-x kostenfrei https://doaj.org/toc/1756-6606 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2019 1 3
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allfieldsGer	10.1186/s13041-019-0476-x doi (DE-627)DOAJ035180277 (DE-599)DOAJ8fe9de2ee9f048829bf10ee546732c30 DE-627 ger DE-627 rakwb eng RC346-429 Michihiro Igarashi verfasserin aut Evolutionary analysis of proline-directed phosphorylation sites in the mammalian growth cone identified using phosphoproteomics 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The growth cone is essential for nerve growth and axon regeneration, which directly form and rearrange the neural network. Recently, to clarify the molecular signaling pathways in the growth cone that utilize protein phosphorylation, we performed a phosphoproteomics study of mammalian growth cone membranes derived from the developing rodent brain and identified < 30,000 phosphopeptides from ~ 1200 proteins. We found that the phosphorylation sites were highly proline directed and primarily mitogen-activated protein kinase (MAPK) dependent, due to particular activation of c-jun N-terminal protein kinase (JNK), a member of the MAPK family. Because the MAPK/JNK pathway is also involved in axon regeneration of invertebrate model organisms such Caenorhabditis elegans and Drosophila, we performed evolutionary bioinformatics analysis of the mammalian growth cone phosphorylation sites. Although these sites were generally conserved within vertebrates, they were not necessarily conserved in these invertebrate model organisms. In particular, high-frequency phosphorylation sites (< 20 times) were less conserved than low-frequency sites. Taken together, the mammalian growth cones contain a large number of vertebrate-specific phosphorylation sites and stronger dependence upon MAPK/JNK than C. elegans or Drosophila. We conclude that axon growth/regeneration likely involves many vertebrate-specific phosphorylation sites. Growth cone Phosphoproteomics Bioinformatics MAPK Evolution Vertebrates Neurology. Diseases of the nervous system Shujiro Okuda verfasserin aut In Molecular Brain BMC, 2010 12(2019), 1, Seite 3 (DE-627)571612873 (DE-600)2436057-0 17566606 nnns volume:12 year:2019 number:1 pages:3 https://doi.org/10.1186/s13041-019-0476-x kostenfrei https://doaj.org/article/8fe9de2ee9f048829bf10ee546732c30 kostenfrei http://link.springer.com/article/10.1186/s13041-019-0476-x kostenfrei https://doaj.org/toc/1756-6606 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2019 1 3
allfieldsSound	10.1186/s13041-019-0476-x doi (DE-627)DOAJ035180277 (DE-599)DOAJ8fe9de2ee9f048829bf10ee546732c30 DE-627 ger DE-627 rakwb eng RC346-429 Michihiro Igarashi verfasserin aut Evolutionary analysis of proline-directed phosphorylation sites in the mammalian growth cone identified using phosphoproteomics 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The growth cone is essential for nerve growth and axon regeneration, which directly form and rearrange the neural network. Recently, to clarify the molecular signaling pathways in the growth cone that utilize protein phosphorylation, we performed a phosphoproteomics study of mammalian growth cone membranes derived from the developing rodent brain and identified < 30,000 phosphopeptides from ~ 1200 proteins. We found that the phosphorylation sites were highly proline directed and primarily mitogen-activated protein kinase (MAPK) dependent, due to particular activation of c-jun N-terminal protein kinase (JNK), a member of the MAPK family. Because the MAPK/JNK pathway is also involved in axon regeneration of invertebrate model organisms such Caenorhabditis elegans and Drosophila, we performed evolutionary bioinformatics analysis of the mammalian growth cone phosphorylation sites. Although these sites were generally conserved within vertebrates, they were not necessarily conserved in these invertebrate model organisms. In particular, high-frequency phosphorylation sites (< 20 times) were less conserved than low-frequency sites. Taken together, the mammalian growth cones contain a large number of vertebrate-specific phosphorylation sites and stronger dependence upon MAPK/JNK than C. elegans or Drosophila. We conclude that axon growth/regeneration likely involves many vertebrate-specific phosphorylation sites. Growth cone Phosphoproteomics Bioinformatics MAPK Evolution Vertebrates Neurology. Diseases of the nervous system Shujiro Okuda verfasserin aut In Molecular Brain BMC, 2010 12(2019), 1, Seite 3 (DE-627)571612873 (DE-600)2436057-0 17566606 nnns volume:12 year:2019 number:1 pages:3 https://doi.org/10.1186/s13041-019-0476-x kostenfrei https://doaj.org/article/8fe9de2ee9f048829bf10ee546732c30 kostenfrei http://link.springer.com/article/10.1186/s13041-019-0476-x kostenfrei https://doaj.org/toc/1756-6606 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2019 1 3
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topic_facet	Growth cone Phosphoproteomics Bioinformatics MAPK Evolution Vertebrates Neurology. Diseases of the nervous system
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callnumber-first	R - Medicine
author	Michihiro Igarashi
spellingShingle	Michihiro Igarashi misc RC346-429 misc Growth cone misc Phosphoproteomics misc Bioinformatics misc MAPK misc Evolution misc Vertebrates misc Neurology. Diseases of the nervous system Evolutionary analysis of proline-directed phosphorylation sites in the mammalian growth cone identified using phosphoproteomics
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topic_title	RC346-429 Evolutionary analysis of proline-directed phosphorylation sites in the mammalian growth cone identified using phosphoproteomics Growth cone Phosphoproteomics Bioinformatics MAPK Evolution Vertebrates
topic	misc RC346-429 misc Growth cone misc Phosphoproteomics misc Bioinformatics misc MAPK misc Evolution misc Vertebrates misc Neurology. Diseases of the nervous system
topic_unstemmed	misc RC346-429 misc Growth cone misc Phosphoproteomics misc Bioinformatics misc MAPK misc Evolution misc Vertebrates misc Neurology. Diseases of the nervous system
topic_browse	misc RC346-429 misc Growth cone misc Phosphoproteomics misc Bioinformatics misc MAPK misc Evolution misc Vertebrates misc Neurology. Diseases of the nervous system
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title	Evolutionary analysis of proline-directed phosphorylation sites in the mammalian growth cone identified using phosphoproteomics
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title_full	Evolutionary analysis of proline-directed phosphorylation sites in the mammalian growth cone identified using phosphoproteomics
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author_browse	Michihiro Igarashi Shujiro Okuda
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title_sort	evolutionary analysis of proline-directed phosphorylation sites in the mammalian growth cone identified using phosphoproteomics
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title_auth	Evolutionary analysis of proline-directed phosphorylation sites in the mammalian growth cone identified using phosphoproteomics
abstract	Abstract The growth cone is essential for nerve growth and axon regeneration, which directly form and rearrange the neural network. Recently, to clarify the molecular signaling pathways in the growth cone that utilize protein phosphorylation, we performed a phosphoproteomics study of mammalian growth cone membranes derived from the developing rodent brain and identified < 30,000 phosphopeptides from ~ 1200 proteins. We found that the phosphorylation sites were highly proline directed and primarily mitogen-activated protein kinase (MAPK) dependent, due to particular activation of c-jun N-terminal protein kinase (JNK), a member of the MAPK family. Because the MAPK/JNK pathway is also involved in axon regeneration of invertebrate model organisms such Caenorhabditis elegans and Drosophila, we performed evolutionary bioinformatics analysis of the mammalian growth cone phosphorylation sites. Although these sites were generally conserved within vertebrates, they were not necessarily conserved in these invertebrate model organisms. In particular, high-frequency phosphorylation sites (< 20 times) were less conserved than low-frequency sites. Taken together, the mammalian growth cones contain a large number of vertebrate-specific phosphorylation sites and stronger dependence upon MAPK/JNK than C. elegans or Drosophila. We conclude that axon growth/regeneration likely involves many vertebrate-specific phosphorylation sites.
abstractGer	Abstract The growth cone is essential for nerve growth and axon regeneration, which directly form and rearrange the neural network. Recently, to clarify the molecular signaling pathways in the growth cone that utilize protein phosphorylation, we performed a phosphoproteomics study of mammalian growth cone membranes derived from the developing rodent brain and identified < 30,000 phosphopeptides from ~ 1200 proteins. We found that the phosphorylation sites were highly proline directed and primarily mitogen-activated protein kinase (MAPK) dependent, due to particular activation of c-jun N-terminal protein kinase (JNK), a member of the MAPK family. Because the MAPK/JNK pathway is also involved in axon regeneration of invertebrate model organisms such Caenorhabditis elegans and Drosophila, we performed evolutionary bioinformatics analysis of the mammalian growth cone phosphorylation sites. Although these sites were generally conserved within vertebrates, they were not necessarily conserved in these invertebrate model organisms. In particular, high-frequency phosphorylation sites (< 20 times) were less conserved than low-frequency sites. Taken together, the mammalian growth cones contain a large number of vertebrate-specific phosphorylation sites and stronger dependence upon MAPK/JNK than C. elegans or Drosophila. We conclude that axon growth/regeneration likely involves many vertebrate-specific phosphorylation sites.
abstract_unstemmed	Abstract The growth cone is essential for nerve growth and axon regeneration, which directly form and rearrange the neural network. Recently, to clarify the molecular signaling pathways in the growth cone that utilize protein phosphorylation, we performed a phosphoproteomics study of mammalian growth cone membranes derived from the developing rodent brain and identified < 30,000 phosphopeptides from ~ 1200 proteins. We found that the phosphorylation sites were highly proline directed and primarily mitogen-activated protein kinase (MAPK) dependent, due to particular activation of c-jun N-terminal protein kinase (JNK), a member of the MAPK family. Because the MAPK/JNK pathway is also involved in axon regeneration of invertebrate model organisms such Caenorhabditis elegans and Drosophila, we performed evolutionary bioinformatics analysis of the mammalian growth cone phosphorylation sites. Although these sites were generally conserved within vertebrates, they were not necessarily conserved in these invertebrate model organisms. In particular, high-frequency phosphorylation sites (< 20 times) were less conserved than low-frequency sites. Taken together, the mammalian growth cones contain a large number of vertebrate-specific phosphorylation sites and stronger dependence upon MAPK/JNK than C. elegans or Drosophila. We conclude that axon growth/regeneration likely involves many vertebrate-specific phosphorylation sites.
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title_short	Evolutionary analysis of proline-directed phosphorylation sites in the mammalian growth cone identified using phosphoproteomics
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Evolutionary analysis of proline-directed phosphorylation sites in the mammalian growth cone identified using phosphoproteomics

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