Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines

<p<Abstract</p< <p<Background</p< <p<Cancer stem cells (CSCs) are regarded as the cause of tumor formation and recurrence. The isolation and identification of CSCs could help to develop novel therapeutic strategies specifically targeting CSCs.</p< <p<Methods...
Ausführliche Beschreibung

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Autor*in:	Chen Lei [verfasserIn] Zhang Yu [verfasserIn] Li Jing [verfasserIn] Zhang Ruixiu [verfasserIn] Xu Wen [verfasserIn] Liao Jian [verfasserIn] Zhai Beibei [verfasserIn] Zhou Yanming [verfasserIn] Cao Lu [verfasserIn] Qian Haihua [verfasserIn] Wu Mengchao [verfasserIn] Yin Zhengfeng [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2011

Übergeordnetes Werk:	In: BMC Gastroenterology - BMC, 2003, 11(2011), 1, p 71
Übergeordnetes Werk:	volume:11 ; year:2011 ; number:1, p 71

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1471-230X-11-71

Katalog-ID:	DOAJ035365129

Internformat


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520			\|a <p<Abstract</p< <p<Background</p< <p<Cancer stem cells (CSCs) are regarded as the cause of tumor formation and recurrence. The isolation and identification of CSCs could help to develop novel therapeutic strategies specifically targeting CSCs.</p< <p<Methods</p< <p<Human hepatoma cell lines were plated in stem cell conditioned culture system allowed for sphere forming. To evaluate the stemness characteristics of spheres, the self-renewal, proliferation, chemoresistance, tumorigenicity of the PLC/PRF/5 sphere-forming cells, and the expression levels of stem cell related proteins in the PLC/PRF/5 sphere-forming cells were assessed, comparing with the parental cells. The stem cell RT-PCR array was performed to further explore the biological properties of liver CSCs.</p< <p<Results</p< <p<The PLC/PRF/5, MHCC97H and HepG2 cells could form clonal nonadherent 3-D spheres and be serially passaged. The PLC/PRF/5 sphere-forming cells possessed a key criteria that define CSCs: persistent self-renewal, extensive proliferation, drug resistance, overexpression of liver CSCs related proteins (Oct3/4, OV6, EpCAM, CD133 and CD44). Even 500 sphere-forming cells were able to form tumors in NOD/SCID mice, and the tumor initiating capability was not decreased when spheres were passaged. Besides, downstream proteins DTX1 and Ep300 of the CSL (CBF1 in humans, Suppressor of hairless in Drosophila and LAG1 in C. elegans) -independent Notch signaling pathway were highly expressed in the spheres, and a gamma-secretase inhibitor MRK003 could significantly inhibit the sphere formation ability.</p< <p<Conclusions</p< <p<Nonadherent tumor spheres from hepatoma cell lines cultured in stem cell conditioned medium possess liver CSC properties, and the CSL-independent Notch signaling pathway may play a role in liver CSCs.</p<
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matchkey_str	article:1471230X:2011----::peeomnclsbouainwtcneseclpoetei
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allfields	10.1186/1471-230X-11-71 doi (DE-627)DOAJ035365129 (DE-599)DOAJ01c00f22c2854aa2b1684ad1f1b842c9 DE-627 ger DE-627 rakwb eng RC799-869 Chen Lei verfasserin aut Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Cancer stem cells (CSCs) are regarded as the cause of tumor formation and recurrence. The isolation and identification of CSCs could help to develop novel therapeutic strategies specifically targeting CSCs.</p< <p<Methods</p< <p<Human hepatoma cell lines were plated in stem cell conditioned culture system allowed for sphere forming. To evaluate the stemness characteristics of spheres, the self-renewal, proliferation, chemoresistance, tumorigenicity of the PLC/PRF/5 sphere-forming cells, and the expression levels of stem cell related proteins in the PLC/PRF/5 sphere-forming cells were assessed, comparing with the parental cells. The stem cell RT-PCR array was performed to further explore the biological properties of liver CSCs.</p< <p<Results</p< <p<The PLC/PRF/5, MHCC97H and HepG2 cells could form clonal nonadherent 3-D spheres and be serially passaged. The PLC/PRF/5 sphere-forming cells possessed a key criteria that define CSCs: persistent self-renewal, extensive proliferation, drug resistance, overexpression of liver CSCs related proteins (Oct3/4, OV6, EpCAM, CD133 and CD44). Even 500 sphere-forming cells were able to form tumors in NOD/SCID mice, and the tumor initiating capability was not decreased when spheres were passaged. Besides, downstream proteins DTX1 and Ep300 of the CSL (CBF1 in humans, Suppressor of hairless in Drosophila and LAG1 in C. elegans) -independent Notch signaling pathway were highly expressed in the spheres, and a gamma-secretase inhibitor MRK003 could significantly inhibit the sphere formation ability.</p< <p<Conclusions</p< <p<Nonadherent tumor spheres from hepatoma cell lines cultured in stem cell conditioned medium possess liver CSC properties, and the CSL-independent Notch signaling pathway may play a role in liver CSCs.</p< Diseases of the digestive system. Gastroenterology Zhang Yu verfasserin aut Li Jing verfasserin aut Zhang Ruixiu verfasserin aut Xu Wen verfasserin aut Liao Jian verfasserin aut Zhai Beibei verfasserin aut Zhou Yanming verfasserin aut Cao Lu verfasserin aut Qian Haihua verfasserin aut Wu Mengchao verfasserin aut Yin Zhengfeng verfasserin aut In BMC Gastroenterology BMC, 2003 11(2011), 1, p 71 (DE-627)326643702 (DE-600)2041351-8 1471230X nnns volume:11 year:2011 number:1, p 71 https://doi.org/10.1186/1471-230X-11-71 kostenfrei https://doaj.org/article/01c00f22c2854aa2b1684ad1f1b842c9 kostenfrei http://www.biomedcentral.com/1471-230X/11/71 kostenfrei https://doaj.org/toc/1471-230X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1, p 71
spelling	10.1186/1471-230X-11-71 doi (DE-627)DOAJ035365129 (DE-599)DOAJ01c00f22c2854aa2b1684ad1f1b842c9 DE-627 ger DE-627 rakwb eng RC799-869 Chen Lei verfasserin aut Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Cancer stem cells (CSCs) are regarded as the cause of tumor formation and recurrence. The isolation and identification of CSCs could help to develop novel therapeutic strategies specifically targeting CSCs.</p< <p<Methods</p< <p<Human hepatoma cell lines were plated in stem cell conditioned culture system allowed for sphere forming. To evaluate the stemness characteristics of spheres, the self-renewal, proliferation, chemoresistance, tumorigenicity of the PLC/PRF/5 sphere-forming cells, and the expression levels of stem cell related proteins in the PLC/PRF/5 sphere-forming cells were assessed, comparing with the parental cells. The stem cell RT-PCR array was performed to further explore the biological properties of liver CSCs.</p< <p<Results</p< <p<The PLC/PRF/5, MHCC97H and HepG2 cells could form clonal nonadherent 3-D spheres and be serially passaged. The PLC/PRF/5 sphere-forming cells possessed a key criteria that define CSCs: persistent self-renewal, extensive proliferation, drug resistance, overexpression of liver CSCs related proteins (Oct3/4, OV6, EpCAM, CD133 and CD44). Even 500 sphere-forming cells were able to form tumors in NOD/SCID mice, and the tumor initiating capability was not decreased when spheres were passaged. Besides, downstream proteins DTX1 and Ep300 of the CSL (CBF1 in humans, Suppressor of hairless in Drosophila and LAG1 in C. elegans) -independent Notch signaling pathway were highly expressed in the spheres, and a gamma-secretase inhibitor MRK003 could significantly inhibit the sphere formation ability.</p< <p<Conclusions</p< <p<Nonadherent tumor spheres from hepatoma cell lines cultured in stem cell conditioned medium possess liver CSC properties, and the CSL-independent Notch signaling pathway may play a role in liver CSCs.</p< Diseases of the digestive system. Gastroenterology Zhang Yu verfasserin aut Li Jing verfasserin aut Zhang Ruixiu verfasserin aut Xu Wen verfasserin aut Liao Jian verfasserin aut Zhai Beibei verfasserin aut Zhou Yanming verfasserin aut Cao Lu verfasserin aut Qian Haihua verfasserin aut Wu Mengchao verfasserin aut Yin Zhengfeng verfasserin aut In BMC Gastroenterology BMC, 2003 11(2011), 1, p 71 (DE-627)326643702 (DE-600)2041351-8 1471230X nnns volume:11 year:2011 number:1, p 71 https://doi.org/10.1186/1471-230X-11-71 kostenfrei https://doaj.org/article/01c00f22c2854aa2b1684ad1f1b842c9 kostenfrei http://www.biomedcentral.com/1471-230X/11/71 kostenfrei https://doaj.org/toc/1471-230X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1, p 71
allfields_unstemmed	10.1186/1471-230X-11-71 doi (DE-627)DOAJ035365129 (DE-599)DOAJ01c00f22c2854aa2b1684ad1f1b842c9 DE-627 ger DE-627 rakwb eng RC799-869 Chen Lei verfasserin aut Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Cancer stem cells (CSCs) are regarded as the cause of tumor formation and recurrence. The isolation and identification of CSCs could help to develop novel therapeutic strategies specifically targeting CSCs.</p< <p<Methods</p< <p<Human hepatoma cell lines were plated in stem cell conditioned culture system allowed for sphere forming. To evaluate the stemness characteristics of spheres, the self-renewal, proliferation, chemoresistance, tumorigenicity of the PLC/PRF/5 sphere-forming cells, and the expression levels of stem cell related proteins in the PLC/PRF/5 sphere-forming cells were assessed, comparing with the parental cells. The stem cell RT-PCR array was performed to further explore the biological properties of liver CSCs.</p< <p<Results</p< <p<The PLC/PRF/5, MHCC97H and HepG2 cells could form clonal nonadherent 3-D spheres and be serially passaged. The PLC/PRF/5 sphere-forming cells possessed a key criteria that define CSCs: persistent self-renewal, extensive proliferation, drug resistance, overexpression of liver CSCs related proteins (Oct3/4, OV6, EpCAM, CD133 and CD44). Even 500 sphere-forming cells were able to form tumors in NOD/SCID mice, and the tumor initiating capability was not decreased when spheres were passaged. Besides, downstream proteins DTX1 and Ep300 of the CSL (CBF1 in humans, Suppressor of hairless in Drosophila and LAG1 in C. elegans) -independent Notch signaling pathway were highly expressed in the spheres, and a gamma-secretase inhibitor MRK003 could significantly inhibit the sphere formation ability.</p< <p<Conclusions</p< <p<Nonadherent tumor spheres from hepatoma cell lines cultured in stem cell conditioned medium possess liver CSC properties, and the CSL-independent Notch signaling pathway may play a role in liver CSCs.</p< Diseases of the digestive system. Gastroenterology Zhang Yu verfasserin aut Li Jing verfasserin aut Zhang Ruixiu verfasserin aut Xu Wen verfasserin aut Liao Jian verfasserin aut Zhai Beibei verfasserin aut Zhou Yanming verfasserin aut Cao Lu verfasserin aut Qian Haihua verfasserin aut Wu Mengchao verfasserin aut Yin Zhengfeng verfasserin aut In BMC Gastroenterology BMC, 2003 11(2011), 1, p 71 (DE-627)326643702 (DE-600)2041351-8 1471230X nnns volume:11 year:2011 number:1, p 71 https://doi.org/10.1186/1471-230X-11-71 kostenfrei https://doaj.org/article/01c00f22c2854aa2b1684ad1f1b842c9 kostenfrei http://www.biomedcentral.com/1471-230X/11/71 kostenfrei https://doaj.org/toc/1471-230X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1, p 71
allfieldsGer	10.1186/1471-230X-11-71 doi (DE-627)DOAJ035365129 (DE-599)DOAJ01c00f22c2854aa2b1684ad1f1b842c9 DE-627 ger DE-627 rakwb eng RC799-869 Chen Lei verfasserin aut Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Cancer stem cells (CSCs) are regarded as the cause of tumor formation and recurrence. The isolation and identification of CSCs could help to develop novel therapeutic strategies specifically targeting CSCs.</p< <p<Methods</p< <p<Human hepatoma cell lines were plated in stem cell conditioned culture system allowed for sphere forming. To evaluate the stemness characteristics of spheres, the self-renewal, proliferation, chemoresistance, tumorigenicity of the PLC/PRF/5 sphere-forming cells, and the expression levels of stem cell related proteins in the PLC/PRF/5 sphere-forming cells were assessed, comparing with the parental cells. The stem cell RT-PCR array was performed to further explore the biological properties of liver CSCs.</p< <p<Results</p< <p<The PLC/PRF/5, MHCC97H and HepG2 cells could form clonal nonadherent 3-D spheres and be serially passaged. The PLC/PRF/5 sphere-forming cells possessed a key criteria that define CSCs: persistent self-renewal, extensive proliferation, drug resistance, overexpression of liver CSCs related proteins (Oct3/4, OV6, EpCAM, CD133 and CD44). Even 500 sphere-forming cells were able to form tumors in NOD/SCID mice, and the tumor initiating capability was not decreased when spheres were passaged. Besides, downstream proteins DTX1 and Ep300 of the CSL (CBF1 in humans, Suppressor of hairless in Drosophila and LAG1 in C. elegans) -independent Notch signaling pathway were highly expressed in the spheres, and a gamma-secretase inhibitor MRK003 could significantly inhibit the sphere formation ability.</p< <p<Conclusions</p< <p<Nonadherent tumor spheres from hepatoma cell lines cultured in stem cell conditioned medium possess liver CSC properties, and the CSL-independent Notch signaling pathway may play a role in liver CSCs.</p< Diseases of the digestive system. Gastroenterology Zhang Yu verfasserin aut Li Jing verfasserin aut Zhang Ruixiu verfasserin aut Xu Wen verfasserin aut Liao Jian verfasserin aut Zhai Beibei verfasserin aut Zhou Yanming verfasserin aut Cao Lu verfasserin aut Qian Haihua verfasserin aut Wu Mengchao verfasserin aut Yin Zhengfeng verfasserin aut In BMC Gastroenterology BMC, 2003 11(2011), 1, p 71 (DE-627)326643702 (DE-600)2041351-8 1471230X nnns volume:11 year:2011 number:1, p 71 https://doi.org/10.1186/1471-230X-11-71 kostenfrei https://doaj.org/article/01c00f22c2854aa2b1684ad1f1b842c9 kostenfrei http://www.biomedcentral.com/1471-230X/11/71 kostenfrei https://doaj.org/toc/1471-230X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1, p 71
allfieldsSound	10.1186/1471-230X-11-71 doi (DE-627)DOAJ035365129 (DE-599)DOAJ01c00f22c2854aa2b1684ad1f1b842c9 DE-627 ger DE-627 rakwb eng RC799-869 Chen Lei verfasserin aut Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Cancer stem cells (CSCs) are regarded as the cause of tumor formation and recurrence. The isolation and identification of CSCs could help to develop novel therapeutic strategies specifically targeting CSCs.</p< <p<Methods</p< <p<Human hepatoma cell lines were plated in stem cell conditioned culture system allowed for sphere forming. To evaluate the stemness characteristics of spheres, the self-renewal, proliferation, chemoresistance, tumorigenicity of the PLC/PRF/5 sphere-forming cells, and the expression levels of stem cell related proteins in the PLC/PRF/5 sphere-forming cells were assessed, comparing with the parental cells. The stem cell RT-PCR array was performed to further explore the biological properties of liver CSCs.</p< <p<Results</p< <p<The PLC/PRF/5, MHCC97H and HepG2 cells could form clonal nonadherent 3-D spheres and be serially passaged. The PLC/PRF/5 sphere-forming cells possessed a key criteria that define CSCs: persistent self-renewal, extensive proliferation, drug resistance, overexpression of liver CSCs related proteins (Oct3/4, OV6, EpCAM, CD133 and CD44). Even 500 sphere-forming cells were able to form tumors in NOD/SCID mice, and the tumor initiating capability was not decreased when spheres were passaged. Besides, downstream proteins DTX1 and Ep300 of the CSL (CBF1 in humans, Suppressor of hairless in Drosophila and LAG1 in C. elegans) -independent Notch signaling pathway were highly expressed in the spheres, and a gamma-secretase inhibitor MRK003 could significantly inhibit the sphere formation ability.</p< <p<Conclusions</p< <p<Nonadherent tumor spheres from hepatoma cell lines cultured in stem cell conditioned medium possess liver CSC properties, and the CSL-independent Notch signaling pathway may play a role in liver CSCs.</p< Diseases of the digestive system. Gastroenterology Zhang Yu verfasserin aut Li Jing verfasserin aut Zhang Ruixiu verfasserin aut Xu Wen verfasserin aut Liao Jian verfasserin aut Zhai Beibei verfasserin aut Zhou Yanming verfasserin aut Cao Lu verfasserin aut Qian Haihua verfasserin aut Wu Mengchao verfasserin aut Yin Zhengfeng verfasserin aut In BMC Gastroenterology BMC, 2003 11(2011), 1, p 71 (DE-627)326643702 (DE-600)2041351-8 1471230X nnns volume:11 year:2011 number:1, p 71 https://doi.org/10.1186/1471-230X-11-71 kostenfrei https://doaj.org/article/01c00f22c2854aa2b1684ad1f1b842c9 kostenfrei http://www.biomedcentral.com/1471-230X/11/71 kostenfrei https://doaj.org/toc/1471-230X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1, p 71
language	English
source	In BMC Gastroenterology 11(2011), 1, p 71 volume:11 year:2011 number:1, p 71
sourceStr	In BMC Gastroenterology 11(2011), 1, p 71 volume:11 year:2011 number:1, p 71
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Diseases of the digestive system. Gastroenterology
isfreeaccess_bool	true
container_title	BMC Gastroenterology
authorswithroles_txt_mv	Chen Lei @@aut@@ Zhang Yu @@aut@@ Li Jing @@aut@@ Zhang Ruixiu @@aut@@ Xu Wen @@aut@@ Liao Jian @@aut@@ Zhai Beibei @@aut@@ Zhou Yanming @@aut@@ Cao Lu @@aut@@ Qian Haihua @@aut@@ Wu Mengchao @@aut@@ Yin Zhengfeng @@aut@@
publishDateDaySort_date	2011-01-01T00:00:00Z
hierarchy_top_id	326643702
id	DOAJ035365129
language_de	englisch
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The PLC/PRF/5 sphere-forming cells possessed a key criteria that define CSCs: persistent self-renewal, extensive proliferation, drug resistance, overexpression of liver CSCs related proteins (Oct3/4, OV6, EpCAM, CD133 and CD44). Even 500 sphere-forming cells were able to form tumors in NOD/SCID mice, and the tumor initiating capability was not decreased when spheres were passaged. 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callnumber-first	R - Medicine
author	Chen Lei
spellingShingle	Chen Lei misc RC799-869 misc Diseases of the digestive system. Gastroenterology Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines
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topic_title	RC799-869 Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines
topic	misc RC799-869 misc Diseases of the digestive system. Gastroenterology
topic_unstemmed	misc RC799-869 misc Diseases of the digestive system. Gastroenterology
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title	Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines
ctrlnum	(DE-627)DOAJ035365129 (DE-599)DOAJ01c00f22c2854aa2b1684ad1f1b842c9
title_full	Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines
author_sort	Chen Lei
journal	BMC Gastroenterology
journalStr	BMC Gastroenterology
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author_browse	Chen Lei Zhang Yu Li Jing Zhang Ruixiu Xu Wen Liao Jian Zhai Beibei Zhou Yanming Cao Lu Qian Haihua Wu Mengchao Yin Zhengfeng
container_volume	11
class	RC799-869
format_se	Elektronische Aufsätze
author-letter	Chen Lei
doi_str_mv	10.1186/1471-230X-11-71
author2-role	verfasserin
title_sort	sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines
callnumber	RC799-869
title_auth	Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines
abstract	<p<Abstract</p< <p<Background</p< <p<Cancer stem cells (CSCs) are regarded as the cause of tumor formation and recurrence. The isolation and identification of CSCs could help to develop novel therapeutic strategies specifically targeting CSCs.</p< <p<Methods</p< <p<Human hepatoma cell lines were plated in stem cell conditioned culture system allowed for sphere forming. To evaluate the stemness characteristics of spheres, the self-renewal, proliferation, chemoresistance, tumorigenicity of the PLC/PRF/5 sphere-forming cells, and the expression levels of stem cell related proteins in the PLC/PRF/5 sphere-forming cells were assessed, comparing with the parental cells. The stem cell RT-PCR array was performed to further explore the biological properties of liver CSCs.</p< <p<Results</p< <p<The PLC/PRF/5, MHCC97H and HepG2 cells could form clonal nonadherent 3-D spheres and be serially passaged. The PLC/PRF/5 sphere-forming cells possessed a key criteria that define CSCs: persistent self-renewal, extensive proliferation, drug resistance, overexpression of liver CSCs related proteins (Oct3/4, OV6, EpCAM, CD133 and CD44). Even 500 sphere-forming cells were able to form tumors in NOD/SCID mice, and the tumor initiating capability was not decreased when spheres were passaged. Besides, downstream proteins DTX1 and Ep300 of the CSL (CBF1 in humans, Suppressor of hairless in Drosophila and LAG1 in C. elegans) -independent Notch signaling pathway were highly expressed in the spheres, and a gamma-secretase inhibitor MRK003 could significantly inhibit the sphere formation ability.</p< <p<Conclusions</p< <p<Nonadherent tumor spheres from hepatoma cell lines cultured in stem cell conditioned medium possess liver CSC properties, and the CSL-independent Notch signaling pathway may play a role in liver CSCs.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<Cancer stem cells (CSCs) are regarded as the cause of tumor formation and recurrence. The isolation and identification of CSCs could help to develop novel therapeutic strategies specifically targeting CSCs.</p< <p<Methods</p< <p<Human hepatoma cell lines were plated in stem cell conditioned culture system allowed for sphere forming. To evaluate the stemness characteristics of spheres, the self-renewal, proliferation, chemoresistance, tumorigenicity of the PLC/PRF/5 sphere-forming cells, and the expression levels of stem cell related proteins in the PLC/PRF/5 sphere-forming cells were assessed, comparing with the parental cells. The stem cell RT-PCR array was performed to further explore the biological properties of liver CSCs.</p< <p<Results</p< <p<The PLC/PRF/5, MHCC97H and HepG2 cells could form clonal nonadherent 3-D spheres and be serially passaged. The PLC/PRF/5 sphere-forming cells possessed a key criteria that define CSCs: persistent self-renewal, extensive proliferation, drug resistance, overexpression of liver CSCs related proteins (Oct3/4, OV6, EpCAM, CD133 and CD44). Even 500 sphere-forming cells were able to form tumors in NOD/SCID mice, and the tumor initiating capability was not decreased when spheres were passaged. Besides, downstream proteins DTX1 and Ep300 of the CSL (CBF1 in humans, Suppressor of hairless in Drosophila and LAG1 in C. elegans) -independent Notch signaling pathway were highly expressed in the spheres, and a gamma-secretase inhibitor MRK003 could significantly inhibit the sphere formation ability.</p< <p<Conclusions</p< <p<Nonadherent tumor spheres from hepatoma cell lines cultured in stem cell conditioned medium possess liver CSC properties, and the CSL-independent Notch signaling pathway may play a role in liver CSCs.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<Cancer stem cells (CSCs) are regarded as the cause of tumor formation and recurrence. The isolation and identification of CSCs could help to develop novel therapeutic strategies specifically targeting CSCs.</p< <p<Methods</p< <p<Human hepatoma cell lines were plated in stem cell conditioned culture system allowed for sphere forming. To evaluate the stemness characteristics of spheres, the self-renewal, proliferation, chemoresistance, tumorigenicity of the PLC/PRF/5 sphere-forming cells, and the expression levels of stem cell related proteins in the PLC/PRF/5 sphere-forming cells were assessed, comparing with the parental cells. The stem cell RT-PCR array was performed to further explore the biological properties of liver CSCs.</p< <p<Results</p< <p<The PLC/PRF/5, MHCC97H and HepG2 cells could form clonal nonadherent 3-D spheres and be serially passaged. The PLC/PRF/5 sphere-forming cells possessed a key criteria that define CSCs: persistent self-renewal, extensive proliferation, drug resistance, overexpression of liver CSCs related proteins (Oct3/4, OV6, EpCAM, CD133 and CD44). Even 500 sphere-forming cells were able to form tumors in NOD/SCID mice, and the tumor initiating capability was not decreased when spheres were passaged. Besides, downstream proteins DTX1 and Ep300 of the CSL (CBF1 in humans, Suppressor of hairless in Drosophila and LAG1 in C. elegans) -independent Notch signaling pathway were highly expressed in the spheres, and a gamma-secretase inhibitor MRK003 could significantly inhibit the sphere formation ability.</p< <p<Conclusions</p< <p<Nonadherent tumor spheres from hepatoma cell lines cultured in stem cell conditioned medium possess liver CSC properties, and the CSL-independent Notch signaling pathway may play a role in liver CSCs.</p<
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container_issue	1, p 71
title_short	Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines
url	https://doi.org/10.1186/1471-230X-11-71 https://doaj.org/article/01c00f22c2854aa2b1684ad1f1b842c9 http://www.biomedcentral.com/1471-230X/11/71 https://doaj.org/toc/1471-230X
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Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines

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