Lgr5-Expressing Cells Are Sufficient and Necessary for Postnatal Mammary Gland Organogenesis

Mammary epithelial stem cells are vital to tissue expansion and remodeling during various phases of postnatal mammary development. Basal mammary epithelial cells are enriched in Wnt-responsive cells and can reconstitute cleared mammary fat pads upon transplantation into mice. Lgr5 is a Wnt-regulated...
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Autor*in:	Vicki Plaks [verfasserIn] Audrey Brenot [verfasserIn] Devon A. Lawson [verfasserIn] Jelena R. Linnemann [verfasserIn] Eline C. Van Kappel [verfasserIn] Karren C. Wong [verfasserIn] Frederic de Sauvage [verfasserIn] Ophir D. Klein [verfasserIn] Zena Werb [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2013

Übergeordnetes Werk:	In: Cell Reports - Elsevier, 2015, 3(2013), 1, Seite 70-78
Übergeordnetes Werk:	volume:3 ; year:2013 ; number:1 ; pages:70-78

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.celrep.2012.12.017

Katalog-ID:	DOAJ035447486

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allfields	10.1016/j.celrep.2012.12.017 doi (DE-627)DOAJ035447486 (DE-599)DOAJc2e7eba37dd74adbadf86b2d712f4323 DE-627 ger DE-627 rakwb eng QH301-705.5 Vicki Plaks verfasserin aut Lgr5-Expressing Cells Are Sufficient and Necessary for Postnatal Mammary Gland Organogenesis 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Mammary epithelial stem cells are vital to tissue expansion and remodeling during various phases of postnatal mammary development. Basal mammary epithelial cells are enriched in Wnt-responsive cells and can reconstitute cleared mammary fat pads upon transplantation into mice. Lgr5 is a Wnt-regulated target gene and was identified as a major stem cell marker in the small intestine, colon, stomach, and hair follicle, as well as in kidney nephrons. Here, we demonstrate the outstanding regenerative potential of a rare population of Lgr5-expressing (Lgr5+) mammary epithelial cells (MECs). We found that Lgr5+ cells reside within the basal population, are superior to other basal cells in regenerating functional mammary glands (MGs), are exceptionally efficient in reconstituting MGs from single cells, and exhibit regenerative capacity in serial transplantations. Loss-of-function and depletion experiments of Lgr5+ cells from transplanted MECs or from pubertal MGs revealed that these cells are not only sufficient but also necessary for postnatal mammary organogenesis. Biology (General) Audrey Brenot verfasserin aut Devon A. Lawson verfasserin aut Jelena R. Linnemann verfasserin aut Eline C. Van Kappel verfasserin aut Karren C. Wong verfasserin aut Frederic de Sauvage verfasserin aut Ophir D. Klein verfasserin aut Zena Werb verfasserin aut In Cell Reports Elsevier, 2015 3(2013), 1, Seite 70-78 (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:3 year:2013 number:1 pages:70-78 https://doi.org/10.1016/j.celrep.2012.12.017 kostenfrei https://doaj.org/article/c2e7eba37dd74adbadf86b2d712f4323 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124713000041 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2013 1 70-78
spelling	10.1016/j.celrep.2012.12.017 doi (DE-627)DOAJ035447486 (DE-599)DOAJc2e7eba37dd74adbadf86b2d712f4323 DE-627 ger DE-627 rakwb eng QH301-705.5 Vicki Plaks verfasserin aut Lgr5-Expressing Cells Are Sufficient and Necessary for Postnatal Mammary Gland Organogenesis 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Mammary epithelial stem cells are vital to tissue expansion and remodeling during various phases of postnatal mammary development. Basal mammary epithelial cells are enriched in Wnt-responsive cells and can reconstitute cleared mammary fat pads upon transplantation into mice. Lgr5 is a Wnt-regulated target gene and was identified as a major stem cell marker in the small intestine, colon, stomach, and hair follicle, as well as in kidney nephrons. Here, we demonstrate the outstanding regenerative potential of a rare population of Lgr5-expressing (Lgr5+) mammary epithelial cells (MECs). We found that Lgr5+ cells reside within the basal population, are superior to other basal cells in regenerating functional mammary glands (MGs), are exceptionally efficient in reconstituting MGs from single cells, and exhibit regenerative capacity in serial transplantations. Loss-of-function and depletion experiments of Lgr5+ cells from transplanted MECs or from pubertal MGs revealed that these cells are not only sufficient but also necessary for postnatal mammary organogenesis. Biology (General) Audrey Brenot verfasserin aut Devon A. Lawson verfasserin aut Jelena R. Linnemann verfasserin aut Eline C. Van Kappel verfasserin aut Karren C. Wong verfasserin aut Frederic de Sauvage verfasserin aut Ophir D. Klein verfasserin aut Zena Werb verfasserin aut In Cell Reports Elsevier, 2015 3(2013), 1, Seite 70-78 (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:3 year:2013 number:1 pages:70-78 https://doi.org/10.1016/j.celrep.2012.12.017 kostenfrei https://doaj.org/article/c2e7eba37dd74adbadf86b2d712f4323 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124713000041 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2013 1 70-78
allfields_unstemmed	10.1016/j.celrep.2012.12.017 doi (DE-627)DOAJ035447486 (DE-599)DOAJc2e7eba37dd74adbadf86b2d712f4323 DE-627 ger DE-627 rakwb eng QH301-705.5 Vicki Plaks verfasserin aut Lgr5-Expressing Cells Are Sufficient and Necessary for Postnatal Mammary Gland Organogenesis 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Mammary epithelial stem cells are vital to tissue expansion and remodeling during various phases of postnatal mammary development. Basal mammary epithelial cells are enriched in Wnt-responsive cells and can reconstitute cleared mammary fat pads upon transplantation into mice. Lgr5 is a Wnt-regulated target gene and was identified as a major stem cell marker in the small intestine, colon, stomach, and hair follicle, as well as in kidney nephrons. Here, we demonstrate the outstanding regenerative potential of a rare population of Lgr5-expressing (Lgr5+) mammary epithelial cells (MECs). We found that Lgr5+ cells reside within the basal population, are superior to other basal cells in regenerating functional mammary glands (MGs), are exceptionally efficient in reconstituting MGs from single cells, and exhibit regenerative capacity in serial transplantations. Loss-of-function and depletion experiments of Lgr5+ cells from transplanted MECs or from pubertal MGs revealed that these cells are not only sufficient but also necessary for postnatal mammary organogenesis. Biology (General) Audrey Brenot verfasserin aut Devon A. Lawson verfasserin aut Jelena R. Linnemann verfasserin aut Eline C. Van Kappel verfasserin aut Karren C. Wong verfasserin aut Frederic de Sauvage verfasserin aut Ophir D. Klein verfasserin aut Zena Werb verfasserin aut In Cell Reports Elsevier, 2015 3(2013), 1, Seite 70-78 (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:3 year:2013 number:1 pages:70-78 https://doi.org/10.1016/j.celrep.2012.12.017 kostenfrei https://doaj.org/article/c2e7eba37dd74adbadf86b2d712f4323 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124713000041 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2013 1 70-78
allfieldsGer	10.1016/j.celrep.2012.12.017 doi (DE-627)DOAJ035447486 (DE-599)DOAJc2e7eba37dd74adbadf86b2d712f4323 DE-627 ger DE-627 rakwb eng QH301-705.5 Vicki Plaks verfasserin aut Lgr5-Expressing Cells Are Sufficient and Necessary for Postnatal Mammary Gland Organogenesis 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Mammary epithelial stem cells are vital to tissue expansion and remodeling during various phases of postnatal mammary development. Basal mammary epithelial cells are enriched in Wnt-responsive cells and can reconstitute cleared mammary fat pads upon transplantation into mice. Lgr5 is a Wnt-regulated target gene and was identified as a major stem cell marker in the small intestine, colon, stomach, and hair follicle, as well as in kidney nephrons. Here, we demonstrate the outstanding regenerative potential of a rare population of Lgr5-expressing (Lgr5+) mammary epithelial cells (MECs). We found that Lgr5+ cells reside within the basal population, are superior to other basal cells in regenerating functional mammary glands (MGs), are exceptionally efficient in reconstituting MGs from single cells, and exhibit regenerative capacity in serial transplantations. Loss-of-function and depletion experiments of Lgr5+ cells from transplanted MECs or from pubertal MGs revealed that these cells are not only sufficient but also necessary for postnatal mammary organogenesis. Biology (General) Audrey Brenot verfasserin aut Devon A. Lawson verfasserin aut Jelena R. Linnemann verfasserin aut Eline C. Van Kappel verfasserin aut Karren C. Wong verfasserin aut Frederic de Sauvage verfasserin aut Ophir D. Klein verfasserin aut Zena Werb verfasserin aut In Cell Reports Elsevier, 2015 3(2013), 1, Seite 70-78 (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:3 year:2013 number:1 pages:70-78 https://doi.org/10.1016/j.celrep.2012.12.017 kostenfrei https://doaj.org/article/c2e7eba37dd74adbadf86b2d712f4323 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124713000041 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2013 1 70-78
allfieldsSound	10.1016/j.celrep.2012.12.017 doi (DE-627)DOAJ035447486 (DE-599)DOAJc2e7eba37dd74adbadf86b2d712f4323 DE-627 ger DE-627 rakwb eng QH301-705.5 Vicki Plaks verfasserin aut Lgr5-Expressing Cells Are Sufficient and Necessary for Postnatal Mammary Gland Organogenesis 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Mammary epithelial stem cells are vital to tissue expansion and remodeling during various phases of postnatal mammary development. Basal mammary epithelial cells are enriched in Wnt-responsive cells and can reconstitute cleared mammary fat pads upon transplantation into mice. Lgr5 is a Wnt-regulated target gene and was identified as a major stem cell marker in the small intestine, colon, stomach, and hair follicle, as well as in kidney nephrons. Here, we demonstrate the outstanding regenerative potential of a rare population of Lgr5-expressing (Lgr5+) mammary epithelial cells (MECs). We found that Lgr5+ cells reside within the basal population, are superior to other basal cells in regenerating functional mammary glands (MGs), are exceptionally efficient in reconstituting MGs from single cells, and exhibit regenerative capacity in serial transplantations. Loss-of-function and depletion experiments of Lgr5+ cells from transplanted MECs or from pubertal MGs revealed that these cells are not only sufficient but also necessary for postnatal mammary organogenesis. Biology (General) Audrey Brenot verfasserin aut Devon A. Lawson verfasserin aut Jelena R. Linnemann verfasserin aut Eline C. Van Kappel verfasserin aut Karren C. Wong verfasserin aut Frederic de Sauvage verfasserin aut Ophir D. Klein verfasserin aut Zena Werb verfasserin aut In Cell Reports Elsevier, 2015 3(2013), 1, Seite 70-78 (DE-627)684964562 (DE-600)2649101-1 22111247 nnns volume:3 year:2013 number:1 pages:70-78 https://doi.org/10.1016/j.celrep.2012.12.017 kostenfrei https://doaj.org/article/c2e7eba37dd74adbadf86b2d712f4323 kostenfrei http://www.sciencedirect.com/science/article/pii/S2211124713000041 kostenfrei https://doaj.org/toc/2211-1247 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2013 1 70-78
language	English
source	In Cell Reports 3(2013), 1, Seite 70-78 volume:3 year:2013 number:1 pages:70-78
sourceStr	In Cell Reports 3(2013), 1, Seite 70-78 volume:3 year:2013 number:1 pages:70-78
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Biology (General)
isfreeaccess_bool	true
container_title	Cell Reports
authorswithroles_txt_mv	Vicki Plaks @@aut@@ Audrey Brenot @@aut@@ Devon A. Lawson @@aut@@ Jelena R. Linnemann @@aut@@ Eline C. Van Kappel @@aut@@ Karren C. Wong @@aut@@ Frederic de Sauvage @@aut@@ Ophir D. Klein @@aut@@ Zena Werb @@aut@@
publishDateDaySort_date	2013-01-01T00:00:00Z
hierarchy_top_id	684964562
id	DOAJ035447486
language_de	englisch
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callnumber-first	Q - Science
author	Vicki Plaks
spellingShingle	Vicki Plaks misc QH301-705.5 misc Biology (General) Lgr5-Expressing Cells Are Sufficient and Necessary for Postnatal Mammary Gland Organogenesis
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topic_title	QH301-705.5 Lgr5-Expressing Cells Are Sufficient and Necessary for Postnatal Mammary Gland Organogenesis
topic	misc QH301-705.5 misc Biology (General)
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title	Lgr5-Expressing Cells Are Sufficient and Necessary for Postnatal Mammary Gland Organogenesis
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title_full	Lgr5-Expressing Cells Are Sufficient and Necessary for Postnatal Mammary Gland Organogenesis
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author_browse	Vicki Plaks Audrey Brenot Devon A. Lawson Jelena R. Linnemann Eline C. Van Kappel Karren C. Wong Frederic de Sauvage Ophir D. Klein Zena Werb
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author-letter	Vicki Plaks
doi_str_mv	10.1016/j.celrep.2012.12.017
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title_sort	lgr5-expressing cells are sufficient and necessary for postnatal mammary gland organogenesis
callnumber	QH301-705.5
title_auth	Lgr5-Expressing Cells Are Sufficient and Necessary for Postnatal Mammary Gland Organogenesis
abstract	Mammary epithelial stem cells are vital to tissue expansion and remodeling during various phases of postnatal mammary development. Basal mammary epithelial cells are enriched in Wnt-responsive cells and can reconstitute cleared mammary fat pads upon transplantation into mice. Lgr5 is a Wnt-regulated target gene and was identified as a major stem cell marker in the small intestine, colon, stomach, and hair follicle, as well as in kidney nephrons. Here, we demonstrate the outstanding regenerative potential of a rare population of Lgr5-expressing (Lgr5+) mammary epithelial cells (MECs). We found that Lgr5+ cells reside within the basal population, are superior to other basal cells in regenerating functional mammary glands (MGs), are exceptionally efficient in reconstituting MGs from single cells, and exhibit regenerative capacity in serial transplantations. Loss-of-function and depletion experiments of Lgr5+ cells from transplanted MECs or from pubertal MGs revealed that these cells are not only sufficient but also necessary for postnatal mammary organogenesis.
abstractGer	Mammary epithelial stem cells are vital to tissue expansion and remodeling during various phases of postnatal mammary development. Basal mammary epithelial cells are enriched in Wnt-responsive cells and can reconstitute cleared mammary fat pads upon transplantation into mice. Lgr5 is a Wnt-regulated target gene and was identified as a major stem cell marker in the small intestine, colon, stomach, and hair follicle, as well as in kidney nephrons. Here, we demonstrate the outstanding regenerative potential of a rare population of Lgr5-expressing (Lgr5+) mammary epithelial cells (MECs). We found that Lgr5+ cells reside within the basal population, are superior to other basal cells in regenerating functional mammary glands (MGs), are exceptionally efficient in reconstituting MGs from single cells, and exhibit regenerative capacity in serial transplantations. Loss-of-function and depletion experiments of Lgr5+ cells from transplanted MECs or from pubertal MGs revealed that these cells are not only sufficient but also necessary for postnatal mammary organogenesis.
abstract_unstemmed	Mammary epithelial stem cells are vital to tissue expansion and remodeling during various phases of postnatal mammary development. Basal mammary epithelial cells are enriched in Wnt-responsive cells and can reconstitute cleared mammary fat pads upon transplantation into mice. Lgr5 is a Wnt-regulated target gene and was identified as a major stem cell marker in the small intestine, colon, stomach, and hair follicle, as well as in kidney nephrons. Here, we demonstrate the outstanding regenerative potential of a rare population of Lgr5-expressing (Lgr5+) mammary epithelial cells (MECs). We found that Lgr5+ cells reside within the basal population, are superior to other basal cells in regenerating functional mammary glands (MGs), are exceptionally efficient in reconstituting MGs from single cells, and exhibit regenerative capacity in serial transplantations. Loss-of-function and depletion experiments of Lgr5+ cells from transplanted MECs or from pubertal MGs revealed that these cells are not only sufficient but also necessary for postnatal mammary organogenesis.
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title_short	Lgr5-Expressing Cells Are Sufficient and Necessary for Postnatal Mammary Gland Organogenesis
url	https://doi.org/10.1016/j.celrep.2012.12.017 https://doaj.org/article/c2e7eba37dd74adbadf86b2d712f4323 http://www.sciencedirect.com/science/article/pii/S2211124713000041 https://doaj.org/toc/2211-1247
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author2	Audrey Brenot Devon A. Lawson Jelena R. Linnemann Eline C. Van Kappel Karren C. Wong Frederic de Sauvage Ophir D. Klein Zena Werb
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up_date	2024-07-03T15:02:19.542Z
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