Mother and child T cell receptor repertoires: deep profiling study

The relationship between maternal and child immunity has been actively studied in the context of complications during pregnancy, autoimmune diseases, and haploidentical transplantation of hematopoietic stem cells (HSC) and solid organs. Here, we have for the first time used high-throughput Illumina...
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Autor*in:	Ekaterina V Putintseva [verfasserIn] Olga V Britanova [verfasserIn] Dmitriy B Staroverov [verfasserIn] Ekaterina M Merzlyak [verfasserIn] Maria A Turchaninova [verfasserIn] M eShugay [verfasserIn] Dmitriy A Bolotin [verfasserIn] Mikhail V Pogorelyy [verfasserIn] Ilgar Z Mamedov [verfasserIn] Vlasta eBobrynina [verfasserIn] Mikhail eMaschan [verfasserIn] Yuri B Lebedev [verfasserIn] Dmitriy M Chudakov [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2013

Schlagwörter:	Autoimmune Diseases Maternal-Fetal Exchange T cell receptor NGS haploidentical transplantation TCR repertoires

Übergeordnetes Werk:	In: Frontiers in Immunology - Frontiers Media S.A., 2011, 4(2013)
Übergeordnetes Werk:	volume:4 ; year:2013

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fimmu.2013.00463

Katalog-ID:	DOAJ035570164

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allfields	10.3389/fimmu.2013.00463 doi (DE-627)DOAJ035570164 (DE-599)DOAJ81c7eead2f67424c8e29517e9e4d894a DE-627 ger DE-627 rakwb eng RC581-607 Ekaterina V Putintseva verfasserin aut Mother and child T cell receptor repertoires: deep profiling study 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The relationship between maternal and child immunity has been actively studied in the context of complications during pregnancy, autoimmune diseases, and haploidentical transplantation of hematopoietic stem cells (HSC) and solid organs. Here, we have for the first time used high-throughput Illumina HiSeq sequencing to perform deep quantitative profiling of T-cell receptor (TCR) repertoires for peripheral blood samples of three mothers and their six children. Advanced technology allowed accurate identification of 5х105–2х106 TCR beta clonotypes per individual. We performed comparative analysis of these TCR repertoires with the aim of revealing characteristic features that distinguish related mother-child pairs, such as relative TRBV segment usage frequency and relative overlap of TCR beta CDR3 repertoires. We show that thymic selection essentially and similarly shapes the initial output of the TCR recombination machinery in both related and unrelated pairs, with minor effect from inherited differences. The achieved depth of TCR profiling also allowed us to test the hypothesis that mature T cells transferred across the placenta during pregnancy can expand and persist as functional microchimeric clones in their new host, using characteristic TCR beta CDR3 variants as clonal identifiers. Autoimmune Diseases Maternal-Fetal Exchange T cell receptor NGS haploidentical transplantation TCR repertoires Immunologic diseases. Allergy Olga V Britanova verfasserin aut Dmitriy B Staroverov verfasserin aut Ekaterina M Merzlyak verfasserin aut Maria A Turchaninova verfasserin aut M eShugay verfasserin aut Dmitriy A Bolotin verfasserin aut Mikhail V Pogorelyy verfasserin aut Ilgar Z Mamedov verfasserin aut Vlasta eBobrynina verfasserin aut Mikhail eMaschan verfasserin aut Yuri B Lebedev verfasserin aut Dmitriy M Chudakov verfasserin aut Dmitriy M Chudakov verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 4(2013) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:4 year:2013 https://doi.org/10.3389/fimmu.2013.00463 kostenfrei https://doaj.org/article/81c7eead2f67424c8e29517e9e4d894a kostenfrei http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00463/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2013
spelling	10.3389/fimmu.2013.00463 doi (DE-627)DOAJ035570164 (DE-599)DOAJ81c7eead2f67424c8e29517e9e4d894a DE-627 ger DE-627 rakwb eng RC581-607 Ekaterina V Putintseva verfasserin aut Mother and child T cell receptor repertoires: deep profiling study 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The relationship between maternal and child immunity has been actively studied in the context of complications during pregnancy, autoimmune diseases, and haploidentical transplantation of hematopoietic stem cells (HSC) and solid organs. Here, we have for the first time used high-throughput Illumina HiSeq sequencing to perform deep quantitative profiling of T-cell receptor (TCR) repertoires for peripheral blood samples of three mothers and their six children. Advanced technology allowed accurate identification of 5х105–2х106 TCR beta clonotypes per individual. We performed comparative analysis of these TCR repertoires with the aim of revealing characteristic features that distinguish related mother-child pairs, such as relative TRBV segment usage frequency and relative overlap of TCR beta CDR3 repertoires. We show that thymic selection essentially and similarly shapes the initial output of the TCR recombination machinery in both related and unrelated pairs, with minor effect from inherited differences. The achieved depth of TCR profiling also allowed us to test the hypothesis that mature T cells transferred across the placenta during pregnancy can expand and persist as functional microchimeric clones in their new host, using characteristic TCR beta CDR3 variants as clonal identifiers. Autoimmune Diseases Maternal-Fetal Exchange T cell receptor NGS haploidentical transplantation TCR repertoires Immunologic diseases. Allergy Olga V Britanova verfasserin aut Dmitriy B Staroverov verfasserin aut Ekaterina M Merzlyak verfasserin aut Maria A Turchaninova verfasserin aut M eShugay verfasserin aut Dmitriy A Bolotin verfasserin aut Mikhail V Pogorelyy verfasserin aut Ilgar Z Mamedov verfasserin aut Vlasta eBobrynina verfasserin aut Mikhail eMaschan verfasserin aut Yuri B Lebedev verfasserin aut Dmitriy M Chudakov verfasserin aut Dmitriy M Chudakov verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 4(2013) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:4 year:2013 https://doi.org/10.3389/fimmu.2013.00463 kostenfrei https://doaj.org/article/81c7eead2f67424c8e29517e9e4d894a kostenfrei http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00463/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2013
allfields_unstemmed	10.3389/fimmu.2013.00463 doi (DE-627)DOAJ035570164 (DE-599)DOAJ81c7eead2f67424c8e29517e9e4d894a DE-627 ger DE-627 rakwb eng RC581-607 Ekaterina V Putintseva verfasserin aut Mother and child T cell receptor repertoires: deep profiling study 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The relationship between maternal and child immunity has been actively studied in the context of complications during pregnancy, autoimmune diseases, and haploidentical transplantation of hematopoietic stem cells (HSC) and solid organs. Here, we have for the first time used high-throughput Illumina HiSeq sequencing to perform deep quantitative profiling of T-cell receptor (TCR) repertoires for peripheral blood samples of three mothers and their six children. Advanced technology allowed accurate identification of 5х105–2х106 TCR beta clonotypes per individual. We performed comparative analysis of these TCR repertoires with the aim of revealing characteristic features that distinguish related mother-child pairs, such as relative TRBV segment usage frequency and relative overlap of TCR beta CDR3 repertoires. We show that thymic selection essentially and similarly shapes the initial output of the TCR recombination machinery in both related and unrelated pairs, with minor effect from inherited differences. The achieved depth of TCR profiling also allowed us to test the hypothesis that mature T cells transferred across the placenta during pregnancy can expand and persist as functional microchimeric clones in their new host, using characteristic TCR beta CDR3 variants as clonal identifiers. Autoimmune Diseases Maternal-Fetal Exchange T cell receptor NGS haploidentical transplantation TCR repertoires Immunologic diseases. Allergy Olga V Britanova verfasserin aut Dmitriy B Staroverov verfasserin aut Ekaterina M Merzlyak verfasserin aut Maria A Turchaninova verfasserin aut M eShugay verfasserin aut Dmitriy A Bolotin verfasserin aut Mikhail V Pogorelyy verfasserin aut Ilgar Z Mamedov verfasserin aut Vlasta eBobrynina verfasserin aut Mikhail eMaschan verfasserin aut Yuri B Lebedev verfasserin aut Dmitriy M Chudakov verfasserin aut Dmitriy M Chudakov verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 4(2013) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:4 year:2013 https://doi.org/10.3389/fimmu.2013.00463 kostenfrei https://doaj.org/article/81c7eead2f67424c8e29517e9e4d894a kostenfrei http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00463/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2013
allfieldsGer	10.3389/fimmu.2013.00463 doi (DE-627)DOAJ035570164 (DE-599)DOAJ81c7eead2f67424c8e29517e9e4d894a DE-627 ger DE-627 rakwb eng RC581-607 Ekaterina V Putintseva verfasserin aut Mother and child T cell receptor repertoires: deep profiling study 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The relationship between maternal and child immunity has been actively studied in the context of complications during pregnancy, autoimmune diseases, and haploidentical transplantation of hematopoietic stem cells (HSC) and solid organs. Here, we have for the first time used high-throughput Illumina HiSeq sequencing to perform deep quantitative profiling of T-cell receptor (TCR) repertoires for peripheral blood samples of three mothers and their six children. Advanced technology allowed accurate identification of 5х105–2х106 TCR beta clonotypes per individual. We performed comparative analysis of these TCR repertoires with the aim of revealing characteristic features that distinguish related mother-child pairs, such as relative TRBV segment usage frequency and relative overlap of TCR beta CDR3 repertoires. We show that thymic selection essentially and similarly shapes the initial output of the TCR recombination machinery in both related and unrelated pairs, with minor effect from inherited differences. The achieved depth of TCR profiling also allowed us to test the hypothesis that mature T cells transferred across the placenta during pregnancy can expand and persist as functional microchimeric clones in their new host, using characteristic TCR beta CDR3 variants as clonal identifiers. Autoimmune Diseases Maternal-Fetal Exchange T cell receptor NGS haploidentical transplantation TCR repertoires Immunologic diseases. Allergy Olga V Britanova verfasserin aut Dmitriy B Staroverov verfasserin aut Ekaterina M Merzlyak verfasserin aut Maria A Turchaninova verfasserin aut M eShugay verfasserin aut Dmitriy A Bolotin verfasserin aut Mikhail V Pogorelyy verfasserin aut Ilgar Z Mamedov verfasserin aut Vlasta eBobrynina verfasserin aut Mikhail eMaschan verfasserin aut Yuri B Lebedev verfasserin aut Dmitriy M Chudakov verfasserin aut Dmitriy M Chudakov verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 4(2013) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:4 year:2013 https://doi.org/10.3389/fimmu.2013.00463 kostenfrei https://doaj.org/article/81c7eead2f67424c8e29517e9e4d894a kostenfrei http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00463/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2013
allfieldsSound	10.3389/fimmu.2013.00463 doi (DE-627)DOAJ035570164 (DE-599)DOAJ81c7eead2f67424c8e29517e9e4d894a DE-627 ger DE-627 rakwb eng RC581-607 Ekaterina V Putintseva verfasserin aut Mother and child T cell receptor repertoires: deep profiling study 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The relationship between maternal and child immunity has been actively studied in the context of complications during pregnancy, autoimmune diseases, and haploidentical transplantation of hematopoietic stem cells (HSC) and solid organs. Here, we have for the first time used high-throughput Illumina HiSeq sequencing to perform deep quantitative profiling of T-cell receptor (TCR) repertoires for peripheral blood samples of three mothers and their six children. Advanced technology allowed accurate identification of 5х105–2х106 TCR beta clonotypes per individual. We performed comparative analysis of these TCR repertoires with the aim of revealing characteristic features that distinguish related mother-child pairs, such as relative TRBV segment usage frequency and relative overlap of TCR beta CDR3 repertoires. We show that thymic selection essentially and similarly shapes the initial output of the TCR recombination machinery in both related and unrelated pairs, with minor effect from inherited differences. The achieved depth of TCR profiling also allowed us to test the hypothesis that mature T cells transferred across the placenta during pregnancy can expand and persist as functional microchimeric clones in their new host, using characteristic TCR beta CDR3 variants as clonal identifiers. Autoimmune Diseases Maternal-Fetal Exchange T cell receptor NGS haploidentical transplantation TCR repertoires Immunologic diseases. Allergy Olga V Britanova verfasserin aut Dmitriy B Staroverov verfasserin aut Ekaterina M Merzlyak verfasserin aut Maria A Turchaninova verfasserin aut M eShugay verfasserin aut Dmitriy A Bolotin verfasserin aut Mikhail V Pogorelyy verfasserin aut Ilgar Z Mamedov verfasserin aut Vlasta eBobrynina verfasserin aut Mikhail eMaschan verfasserin aut Yuri B Lebedev verfasserin aut Dmitriy M Chudakov verfasserin aut Dmitriy M Chudakov verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 4(2013) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:4 year:2013 https://doi.org/10.3389/fimmu.2013.00463 kostenfrei https://doaj.org/article/81c7eead2f67424c8e29517e9e4d894a kostenfrei http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00463/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2013
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topic_facet	Autoimmune Diseases Maternal-Fetal Exchange T cell receptor NGS haploidentical transplantation TCR repertoires Immunologic diseases. Allergy
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container_title	Frontiers in Immunology
authorswithroles_txt_mv	Ekaterina V Putintseva @@aut@@ Olga V Britanova @@aut@@ Dmitriy B Staroverov @@aut@@ Ekaterina M Merzlyak @@aut@@ Maria A Turchaninova @@aut@@ M eShugay @@aut@@ Dmitriy A Bolotin @@aut@@ Mikhail V Pogorelyy @@aut@@ Ilgar Z Mamedov @@aut@@ Vlasta eBobrynina @@aut@@ Mikhail eMaschan @@aut@@ Yuri B Lebedev @@aut@@ Dmitriy M Chudakov @@aut@@
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callnumber-first	R - Medicine
author	Ekaterina V Putintseva
spellingShingle	Ekaterina V Putintseva misc RC581-607 misc Autoimmune Diseases misc Maternal-Fetal Exchange misc T cell receptor misc NGS misc haploidentical transplantation misc TCR repertoires misc Immunologic diseases. Allergy Mother and child T cell receptor repertoires: deep profiling study
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topic_title	RC581-607 Mother and child T cell receptor repertoires: deep profiling study Autoimmune Diseases Maternal-Fetal Exchange T cell receptor NGS haploidentical transplantation TCR repertoires
topic	misc RC581-607 misc Autoimmune Diseases misc Maternal-Fetal Exchange misc T cell receptor misc NGS misc haploidentical transplantation misc TCR repertoires misc Immunologic diseases. Allergy
topic_unstemmed	misc RC581-607 misc Autoimmune Diseases misc Maternal-Fetal Exchange misc T cell receptor misc NGS misc haploidentical transplantation misc TCR repertoires misc Immunologic diseases. Allergy
topic_browse	misc RC581-607 misc Autoimmune Diseases misc Maternal-Fetal Exchange misc T cell receptor misc NGS misc haploidentical transplantation misc TCR repertoires misc Immunologic diseases. Allergy
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title	Mother and child T cell receptor repertoires: deep profiling study
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title_full	Mother and child T cell receptor repertoires: deep profiling study
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author_browse	Ekaterina V Putintseva Olga V Britanova Dmitriy B Staroverov Ekaterina M Merzlyak Maria A Turchaninova M eShugay Dmitriy A Bolotin Mikhail V Pogorelyy Ilgar Z Mamedov Vlasta eBobrynina Mikhail eMaschan Yuri B Lebedev Dmitriy M Chudakov
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title_sort	mother and child t cell receptor repertoires: deep profiling study
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title_auth	Mother and child T cell receptor repertoires: deep profiling study
abstract	The relationship between maternal and child immunity has been actively studied in the context of complications during pregnancy, autoimmune diseases, and haploidentical transplantation of hematopoietic stem cells (HSC) and solid organs. Here, we have for the first time used high-throughput Illumina HiSeq sequencing to perform deep quantitative profiling of T-cell receptor (TCR) repertoires for peripheral blood samples of three mothers and their six children. Advanced technology allowed accurate identification of 5х105–2х106 TCR beta clonotypes per individual. We performed comparative analysis of these TCR repertoires with the aim of revealing characteristic features that distinguish related mother-child pairs, such as relative TRBV segment usage frequency and relative overlap of TCR beta CDR3 repertoires. We show that thymic selection essentially and similarly shapes the initial output of the TCR recombination machinery in both related and unrelated pairs, with minor effect from inherited differences. The achieved depth of TCR profiling also allowed us to test the hypothesis that mature T cells transferred across the placenta during pregnancy can expand and persist as functional microchimeric clones in their new host, using characteristic TCR beta CDR3 variants as clonal identifiers.
abstractGer	The relationship between maternal and child immunity has been actively studied in the context of complications during pregnancy, autoimmune diseases, and haploidentical transplantation of hematopoietic stem cells (HSC) and solid organs. Here, we have for the first time used high-throughput Illumina HiSeq sequencing to perform deep quantitative profiling of T-cell receptor (TCR) repertoires for peripheral blood samples of three mothers and their six children. Advanced technology allowed accurate identification of 5х105–2х106 TCR beta clonotypes per individual. We performed comparative analysis of these TCR repertoires with the aim of revealing characteristic features that distinguish related mother-child pairs, such as relative TRBV segment usage frequency and relative overlap of TCR beta CDR3 repertoires. We show that thymic selection essentially and similarly shapes the initial output of the TCR recombination machinery in both related and unrelated pairs, with minor effect from inherited differences. The achieved depth of TCR profiling also allowed us to test the hypothesis that mature T cells transferred across the placenta during pregnancy can expand and persist as functional microchimeric clones in their new host, using characteristic TCR beta CDR3 variants as clonal identifiers.
abstract_unstemmed	The relationship between maternal and child immunity has been actively studied in the context of complications during pregnancy, autoimmune diseases, and haploidentical transplantation of hematopoietic stem cells (HSC) and solid organs. Here, we have for the first time used high-throughput Illumina HiSeq sequencing to perform deep quantitative profiling of T-cell receptor (TCR) repertoires for peripheral blood samples of three mothers and their six children. Advanced technology allowed accurate identification of 5х105–2х106 TCR beta clonotypes per individual. We performed comparative analysis of these TCR repertoires with the aim of revealing characteristic features that distinguish related mother-child pairs, such as relative TRBV segment usage frequency and relative overlap of TCR beta CDR3 repertoires. We show that thymic selection essentially and similarly shapes the initial output of the TCR recombination machinery in both related and unrelated pairs, with minor effect from inherited differences. The achieved depth of TCR profiling also allowed us to test the hypothesis that mature T cells transferred across the placenta during pregnancy can expand and persist as functional microchimeric clones in their new host, using characteristic TCR beta CDR3 variants as clonal identifiers.
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title_short	Mother and child T cell receptor repertoires: deep profiling study
url	https://doi.org/10.3389/fimmu.2013.00463 https://doaj.org/article/81c7eead2f67424c8e29517e9e4d894a http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00463/full https://doaj.org/toc/1664-3224
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up_date	2024-07-03T15:45:05.190Z
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Mother and child T cell receptor repertoires: deep profiling study

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