Rapamycin ameliorates experimental autoimmune uveoretinitis by inhibiting Th1/Th2/Th17 cells and upregulating CD4+CD25+ Foxp3 regulatory T cells

<b<AIM:</b<To determine the effects of rapamycin on experimental autoimmune uveoretinitis (EAU) and investigate of role of rapamycin on T cell subsets in the disease. <b<METHODS</b<:EAU was induced in rats using peptides 1169 to 1191 of the interphotoreceptor binding protein...
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Gespeichert in:

Autor*in:	Li-Fei Yuan [verfasserIn] Guang-Da Li [verfasserIn] Xin-Jun Ren [verfasserIn] Hong Nian [verfasserIn] Xiao-Rong Li [verfasserIn] Xiao-Min Zhang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	experimental autoimmune uveoretinitis rapamycin regulatory T cells Th1 cells Th2 cells Th17 cells uveitis

Übergeordnetes Werk:	In: International Journal of Ophthalmology - Press of International Journal of Ophthalmology (IJO PRESS), 2016, 8(2015), 4, Seite 659-664
Übergeordnetes Werk:	volume:8 ; year:2015 ; number:4 ; pages:659-664

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3980/j.issn.2222-3959.2015.04.03

Katalog-ID:	DOAJ035625082

Internformat


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520			\|a <b<AIM:</b<To determine the effects of rapamycin on experimental autoimmune uveoretinitis (EAU) and investigate of role of rapamycin on T cell subsets in the disease. <b<METHODS</b<:EAU was induced in rats using peptides 1169 to 1191 of the interphotoreceptor binding protein (IRBP). Rapamycin (0.2 mg/kg/d) was administrated by intraperitoneal injection for a consecutive 7d after immunization. Th1/Th2/Th17 cytokines, TGF-β1, and IL-6 produced by lymphocyteswere measured by ELISA, while Th17 cells and CD4+CD25+ regulatory T cells (Tregs) from rat spleen were detected by flow cytometry. <b<RESULTS:</b< Intraperitoneal treatment immediately after immunization dramatically ameliorated the clinical course of EAU. Clinical responses were associated with reduced retinal inflammatory cell infiltration and tissue destruction. Rapamycin induced suppression of Th1/Th2/Th17 cytokines, including IFN-γ, IL-2, IL-17, IL-4, and IL-10 release from T lymphocytes of EAU rats, <i<in vitro</i<. Rapamycin also significantly increased TGF-β1 production but had no effect on IL-6 productionof T lymphocytes from EAU rats in vitro. Furthermore, rapamycin decreased the ratio of Th17 cells/CD4+T cells and upregulated Tregs in EAU, as detected by flow cytometry. <b<CONCLUSION:</b< Rapamycin effectively interferes with T cell mediated autoimmune uveitis by inhibiting antigen-specific T cell functions and enhancing Tregs in EAU. Rapamycin is a promising new alternative as an adjunct corticosteroid-sparing agent for treating uveitis.
650		4	\|a experimental autoimmune uveoretinitis
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allfields	10.3980/j.issn.2222-3959.2015.04.03 doi (DE-627)DOAJ035625082 (DE-599)DOAJe221db0710f34d949eae343182699d12 DE-627 ger DE-627 rakwb eng RE1-994 Li-Fei Yuan verfasserin aut Rapamycin ameliorates experimental autoimmune uveoretinitis by inhibiting Th1/Th2/Th17 cells and upregulating CD4+CD25+ Foxp3 regulatory T cells 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <b<AIM:</b<To determine the effects of rapamycin on experimental autoimmune uveoretinitis (EAU) and investigate of role of rapamycin on T cell subsets in the disease. <b<METHODS</b<:EAU was induced in rats using peptides 1169 to 1191 of the interphotoreceptor binding protein (IRBP). Rapamycin (0.2 mg/kg/d) was administrated by intraperitoneal injection for a consecutive 7d after immunization. Th1/Th2/Th17 cytokines, TGF-β1, and IL-6 produced by lymphocyteswere measured by ELISA, while Th17 cells and CD4+CD25+ regulatory T cells (Tregs) from rat spleen were detected by flow cytometry. <b<RESULTS:</b< Intraperitoneal treatment immediately after immunization dramatically ameliorated the clinical course of EAU. Clinical responses were associated with reduced retinal inflammatory cell infiltration and tissue destruction. Rapamycin induced suppression of Th1/Th2/Th17 cytokines, including IFN-γ, IL-2, IL-17, IL-4, and IL-10 release from T lymphocytes of EAU rats, <i<in vitro</i<. Rapamycin also significantly increased TGF-β1 production but had no effect on IL-6 productionof T lymphocytes from EAU rats in vitro. Furthermore, rapamycin decreased the ratio of Th17 cells/CD4+T cells and upregulated Tregs in EAU, as detected by flow cytometry. <b<CONCLUSION:</b< Rapamycin effectively interferes with T cell mediated autoimmune uveitis by inhibiting antigen-specific T cell functions and enhancing Tregs in EAU. Rapamycin is a promising new alternative as an adjunct corticosteroid-sparing agent for treating uveitis. experimental autoimmune uveoretinitis rapamycin regulatory T cells Th1 cells Th2 cells Th17 cells uveitis Ophthalmology Guang-Da Li verfasserin aut Xin-Jun Ren verfasserin aut Hong Nian verfasserin aut Xiao-Rong Li verfasserin aut Xiao-Min Zhang verfasserin aut In International Journal of Ophthalmology Press of International Journal of Ophthalmology (IJO PRESS), 2016 8(2015), 4, Seite 659-664 (DE-627)718635906 (DE-600)2663246-9 22274898 nnns volume:8 year:2015 number:4 pages:659-664 https://doi.org/10.3980/j.issn.2222-3959.2015.04.03 kostenfrei https://doaj.org/article/e221db0710f34d949eae343182699d12 kostenfrei http://www.ijo.cn/en_publish/2015/4/20150403.pdf kostenfrei https://doaj.org/toc/2222-3959 Journal toc kostenfrei https://doaj.org/toc/2227-4898 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2015 4 659-664
spelling	10.3980/j.issn.2222-3959.2015.04.03 doi (DE-627)DOAJ035625082 (DE-599)DOAJe221db0710f34d949eae343182699d12 DE-627 ger DE-627 rakwb eng RE1-994 Li-Fei Yuan verfasserin aut Rapamycin ameliorates experimental autoimmune uveoretinitis by inhibiting Th1/Th2/Th17 cells and upregulating CD4+CD25+ Foxp3 regulatory T cells 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <b<AIM:</b<To determine the effects of rapamycin on experimental autoimmune uveoretinitis (EAU) and investigate of role of rapamycin on T cell subsets in the disease. <b<METHODS</b<:EAU was induced in rats using peptides 1169 to 1191 of the interphotoreceptor binding protein (IRBP). Rapamycin (0.2 mg/kg/d) was administrated by intraperitoneal injection for a consecutive 7d after immunization. Th1/Th2/Th17 cytokines, TGF-β1, and IL-6 produced by lymphocyteswere measured by ELISA, while Th17 cells and CD4+CD25+ regulatory T cells (Tregs) from rat spleen were detected by flow cytometry. <b<RESULTS:</b< Intraperitoneal treatment immediately after immunization dramatically ameliorated the clinical course of EAU. Clinical responses were associated with reduced retinal inflammatory cell infiltration and tissue destruction. Rapamycin induced suppression of Th1/Th2/Th17 cytokines, including IFN-γ, IL-2, IL-17, IL-4, and IL-10 release from T lymphocytes of EAU rats, <i<in vitro</i<. Rapamycin also significantly increased TGF-β1 production but had no effect on IL-6 productionof T lymphocytes from EAU rats in vitro. Furthermore, rapamycin decreased the ratio of Th17 cells/CD4+T cells and upregulated Tregs in EAU, as detected by flow cytometry. <b<CONCLUSION:</b< Rapamycin effectively interferes with T cell mediated autoimmune uveitis by inhibiting antigen-specific T cell functions and enhancing Tregs in EAU. Rapamycin is a promising new alternative as an adjunct corticosteroid-sparing agent for treating uveitis. experimental autoimmune uveoretinitis rapamycin regulatory T cells Th1 cells Th2 cells Th17 cells uveitis Ophthalmology Guang-Da Li verfasserin aut Xin-Jun Ren verfasserin aut Hong Nian verfasserin aut Xiao-Rong Li verfasserin aut Xiao-Min Zhang verfasserin aut In International Journal of Ophthalmology Press of International Journal of Ophthalmology (IJO PRESS), 2016 8(2015), 4, Seite 659-664 (DE-627)718635906 (DE-600)2663246-9 22274898 nnns volume:8 year:2015 number:4 pages:659-664 https://doi.org/10.3980/j.issn.2222-3959.2015.04.03 kostenfrei https://doaj.org/article/e221db0710f34d949eae343182699d12 kostenfrei http://www.ijo.cn/en_publish/2015/4/20150403.pdf kostenfrei https://doaj.org/toc/2222-3959 Journal toc kostenfrei https://doaj.org/toc/2227-4898 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2015 4 659-664
allfields_unstemmed	10.3980/j.issn.2222-3959.2015.04.03 doi (DE-627)DOAJ035625082 (DE-599)DOAJe221db0710f34d949eae343182699d12 DE-627 ger DE-627 rakwb eng RE1-994 Li-Fei Yuan verfasserin aut Rapamycin ameliorates experimental autoimmune uveoretinitis by inhibiting Th1/Th2/Th17 cells and upregulating CD4+CD25+ Foxp3 regulatory T cells 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <b<AIM:</b<To determine the effects of rapamycin on experimental autoimmune uveoretinitis (EAU) and investigate of role of rapamycin on T cell subsets in the disease. <b<METHODS</b<:EAU was induced in rats using peptides 1169 to 1191 of the interphotoreceptor binding protein (IRBP). Rapamycin (0.2 mg/kg/d) was administrated by intraperitoneal injection for a consecutive 7d after immunization. Th1/Th2/Th17 cytokines, TGF-β1, and IL-6 produced by lymphocyteswere measured by ELISA, while Th17 cells and CD4+CD25+ regulatory T cells (Tregs) from rat spleen were detected by flow cytometry. <b<RESULTS:</b< Intraperitoneal treatment immediately after immunization dramatically ameliorated the clinical course of EAU. Clinical responses were associated with reduced retinal inflammatory cell infiltration and tissue destruction. Rapamycin induced suppression of Th1/Th2/Th17 cytokines, including IFN-γ, IL-2, IL-17, IL-4, and IL-10 release from T lymphocytes of EAU rats, <i<in vitro</i<. Rapamycin also significantly increased TGF-β1 production but had no effect on IL-6 productionof T lymphocytes from EAU rats in vitro. Furthermore, rapamycin decreased the ratio of Th17 cells/CD4+T cells and upregulated Tregs in EAU, as detected by flow cytometry. <b<CONCLUSION:</b< Rapamycin effectively interferes with T cell mediated autoimmune uveitis by inhibiting antigen-specific T cell functions and enhancing Tregs in EAU. Rapamycin is a promising new alternative as an adjunct corticosteroid-sparing agent for treating uveitis. experimental autoimmune uveoretinitis rapamycin regulatory T cells Th1 cells Th2 cells Th17 cells uveitis Ophthalmology Guang-Da Li verfasserin aut Xin-Jun Ren verfasserin aut Hong Nian verfasserin aut Xiao-Rong Li verfasserin aut Xiao-Min Zhang verfasserin aut In International Journal of Ophthalmology Press of International Journal of Ophthalmology (IJO PRESS), 2016 8(2015), 4, Seite 659-664 (DE-627)718635906 (DE-600)2663246-9 22274898 nnns volume:8 year:2015 number:4 pages:659-664 https://doi.org/10.3980/j.issn.2222-3959.2015.04.03 kostenfrei https://doaj.org/article/e221db0710f34d949eae343182699d12 kostenfrei http://www.ijo.cn/en_publish/2015/4/20150403.pdf kostenfrei https://doaj.org/toc/2222-3959 Journal toc kostenfrei https://doaj.org/toc/2227-4898 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2015 4 659-664
allfieldsGer	10.3980/j.issn.2222-3959.2015.04.03 doi (DE-627)DOAJ035625082 (DE-599)DOAJe221db0710f34d949eae343182699d12 DE-627 ger DE-627 rakwb eng RE1-994 Li-Fei Yuan verfasserin aut Rapamycin ameliorates experimental autoimmune uveoretinitis by inhibiting Th1/Th2/Th17 cells and upregulating CD4+CD25+ Foxp3 regulatory T cells 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <b<AIM:</b<To determine the effects of rapamycin on experimental autoimmune uveoretinitis (EAU) and investigate of role of rapamycin on T cell subsets in the disease. <b<METHODS</b<:EAU was induced in rats using peptides 1169 to 1191 of the interphotoreceptor binding protein (IRBP). Rapamycin (0.2 mg/kg/d) was administrated by intraperitoneal injection for a consecutive 7d after immunization. Th1/Th2/Th17 cytokines, TGF-β1, and IL-6 produced by lymphocyteswere measured by ELISA, while Th17 cells and CD4+CD25+ regulatory T cells (Tregs) from rat spleen were detected by flow cytometry. <b<RESULTS:</b< Intraperitoneal treatment immediately after immunization dramatically ameliorated the clinical course of EAU. Clinical responses were associated with reduced retinal inflammatory cell infiltration and tissue destruction. Rapamycin induced suppression of Th1/Th2/Th17 cytokines, including IFN-γ, IL-2, IL-17, IL-4, and IL-10 release from T lymphocytes of EAU rats, <i<in vitro</i<. Rapamycin also significantly increased TGF-β1 production but had no effect on IL-6 productionof T lymphocytes from EAU rats in vitro. Furthermore, rapamycin decreased the ratio of Th17 cells/CD4+T cells and upregulated Tregs in EAU, as detected by flow cytometry. <b<CONCLUSION:</b< Rapamycin effectively interferes with T cell mediated autoimmune uveitis by inhibiting antigen-specific T cell functions and enhancing Tregs in EAU. Rapamycin is a promising new alternative as an adjunct corticosteroid-sparing agent for treating uveitis. experimental autoimmune uveoretinitis rapamycin regulatory T cells Th1 cells Th2 cells Th17 cells uveitis Ophthalmology Guang-Da Li verfasserin aut Xin-Jun Ren verfasserin aut Hong Nian verfasserin aut Xiao-Rong Li verfasserin aut Xiao-Min Zhang verfasserin aut In International Journal of Ophthalmology Press of International Journal of Ophthalmology (IJO PRESS), 2016 8(2015), 4, Seite 659-664 (DE-627)718635906 (DE-600)2663246-9 22274898 nnns volume:8 year:2015 number:4 pages:659-664 https://doi.org/10.3980/j.issn.2222-3959.2015.04.03 kostenfrei https://doaj.org/article/e221db0710f34d949eae343182699d12 kostenfrei http://www.ijo.cn/en_publish/2015/4/20150403.pdf kostenfrei https://doaj.org/toc/2222-3959 Journal toc kostenfrei https://doaj.org/toc/2227-4898 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2015 4 659-664
allfieldsSound	10.3980/j.issn.2222-3959.2015.04.03 doi (DE-627)DOAJ035625082 (DE-599)DOAJe221db0710f34d949eae343182699d12 DE-627 ger DE-627 rakwb eng RE1-994 Li-Fei Yuan verfasserin aut Rapamycin ameliorates experimental autoimmune uveoretinitis by inhibiting Th1/Th2/Th17 cells and upregulating CD4+CD25+ Foxp3 regulatory T cells 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <b<AIM:</b<To determine the effects of rapamycin on experimental autoimmune uveoretinitis (EAU) and investigate of role of rapamycin on T cell subsets in the disease. <b<METHODS</b<:EAU was induced in rats using peptides 1169 to 1191 of the interphotoreceptor binding protein (IRBP). Rapamycin (0.2 mg/kg/d) was administrated by intraperitoneal injection for a consecutive 7d after immunization. Th1/Th2/Th17 cytokines, TGF-β1, and IL-6 produced by lymphocyteswere measured by ELISA, while Th17 cells and CD4+CD25+ regulatory T cells (Tregs) from rat spleen were detected by flow cytometry. <b<RESULTS:</b< Intraperitoneal treatment immediately after immunization dramatically ameliorated the clinical course of EAU. Clinical responses were associated with reduced retinal inflammatory cell infiltration and tissue destruction. Rapamycin induced suppression of Th1/Th2/Th17 cytokines, including IFN-γ, IL-2, IL-17, IL-4, and IL-10 release from T lymphocytes of EAU rats, <i<in vitro</i<. Rapamycin also significantly increased TGF-β1 production but had no effect on IL-6 productionof T lymphocytes from EAU rats in vitro. Furthermore, rapamycin decreased the ratio of Th17 cells/CD4+T cells and upregulated Tregs in EAU, as detected by flow cytometry. <b<CONCLUSION:</b< Rapamycin effectively interferes with T cell mediated autoimmune uveitis by inhibiting antigen-specific T cell functions and enhancing Tregs in EAU. Rapamycin is a promising new alternative as an adjunct corticosteroid-sparing agent for treating uveitis. experimental autoimmune uveoretinitis rapamycin regulatory T cells Th1 cells Th2 cells Th17 cells uveitis Ophthalmology Guang-Da Li verfasserin aut Xin-Jun Ren verfasserin aut Hong Nian verfasserin aut Xiao-Rong Li verfasserin aut Xiao-Min Zhang verfasserin aut In International Journal of Ophthalmology Press of International Journal of Ophthalmology (IJO PRESS), 2016 8(2015), 4, Seite 659-664 (DE-627)718635906 (DE-600)2663246-9 22274898 nnns volume:8 year:2015 number:4 pages:659-664 https://doi.org/10.3980/j.issn.2222-3959.2015.04.03 kostenfrei https://doaj.org/article/e221db0710f34d949eae343182699d12 kostenfrei http://www.ijo.cn/en_publish/2015/4/20150403.pdf kostenfrei https://doaj.org/toc/2222-3959 Journal toc kostenfrei https://doaj.org/toc/2227-4898 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2015 4 659-664
language	English
source	In International Journal of Ophthalmology 8(2015), 4, Seite 659-664 volume:8 year:2015 number:4 pages:659-664
sourceStr	In International Journal of Ophthalmology 8(2015), 4, Seite 659-664 volume:8 year:2015 number:4 pages:659-664
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topic_facet	experimental autoimmune uveoretinitis rapamycin regulatory T cells Th1 cells Th2 cells Th17 cells uveitis Ophthalmology
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authorswithroles_txt_mv	Li-Fei Yuan @@aut@@ Guang-Da Li @@aut@@ Xin-Jun Ren @@aut@@ Hong Nian @@aut@@ Xiao-Rong Li @@aut@@ Xiao-Min Zhang @@aut@@
publishDateDaySort_date	2015-01-01T00:00:00Z
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callnumber-first	R - Medicine
author	Li-Fei Yuan
spellingShingle	Li-Fei Yuan misc RE1-994 misc experimental autoimmune uveoretinitis misc rapamycin misc regulatory T cells misc Th1 cells misc Th2 cells misc Th17 cells misc uveitis misc Ophthalmology Rapamycin ameliorates experimental autoimmune uveoretinitis by inhibiting Th1/Th2/Th17 cells and upregulating CD4+CD25+ Foxp3 regulatory T cells
authorStr	Li-Fei Yuan
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topic_title	RE1-994 Rapamycin ameliorates experimental autoimmune uveoretinitis by inhibiting Th1/Th2/Th17 cells and upregulating CD4+CD25+ Foxp3 regulatory T cells experimental autoimmune uveoretinitis rapamycin regulatory T cells Th1 cells Th2 cells Th17 cells uveitis
topic	misc RE1-994 misc experimental autoimmune uveoretinitis misc rapamycin misc regulatory T cells misc Th1 cells misc Th2 cells misc Th17 cells misc uveitis misc Ophthalmology
topic_unstemmed	misc RE1-994 misc experimental autoimmune uveoretinitis misc rapamycin misc regulatory T cells misc Th1 cells misc Th2 cells misc Th17 cells misc uveitis misc Ophthalmology
topic_browse	misc RE1-994 misc experimental autoimmune uveoretinitis misc rapamycin misc regulatory T cells misc Th1 cells misc Th2 cells misc Th17 cells misc uveitis misc Ophthalmology
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hierarchy_parent_title	International Journal of Ophthalmology
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title	Rapamycin ameliorates experimental autoimmune uveoretinitis by inhibiting Th1/Th2/Th17 cells and upregulating CD4+CD25+ Foxp3 regulatory T cells
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title_full	Rapamycin ameliorates experimental autoimmune uveoretinitis by inhibiting Th1/Th2/Th17 cells and upregulating CD4+CD25+ Foxp3 regulatory T cells
author_sort	Li-Fei Yuan
journal	International Journal of Ophthalmology
journalStr	International Journal of Ophthalmology
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author_browse	Li-Fei Yuan Guang-Da Li Xin-Jun Ren Hong Nian Xiao-Rong Li Xiao-Min Zhang
container_volume	8
class	RE1-994
format_se	Elektronische Aufsätze
author-letter	Li-Fei Yuan
doi_str_mv	10.3980/j.issn.2222-3959.2015.04.03
author2-role	verfasserin
title_sort	rapamycin ameliorates experimental autoimmune uveoretinitis by inhibiting th1/th2/th17 cells and upregulating cd4+cd25+ foxp3 regulatory t cells
callnumber	RE1-994
title_auth	Rapamycin ameliorates experimental autoimmune uveoretinitis by inhibiting Th1/Th2/Th17 cells and upregulating CD4+CD25+ Foxp3 regulatory T cells
abstract	<b<AIM:</b<To determine the effects of rapamycin on experimental autoimmune uveoretinitis (EAU) and investigate of role of rapamycin on T cell subsets in the disease. <b<METHODS</b<:EAU was induced in rats using peptides 1169 to 1191 of the interphotoreceptor binding protein (IRBP). Rapamycin (0.2 mg/kg/d) was administrated by intraperitoneal injection for a consecutive 7d after immunization. Th1/Th2/Th17 cytokines, TGF-β1, and IL-6 produced by lymphocyteswere measured by ELISA, while Th17 cells and CD4+CD25+ regulatory T cells (Tregs) from rat spleen were detected by flow cytometry. <b<RESULTS:</b< Intraperitoneal treatment immediately after immunization dramatically ameliorated the clinical course of EAU. Clinical responses were associated with reduced retinal inflammatory cell infiltration and tissue destruction. Rapamycin induced suppression of Th1/Th2/Th17 cytokines, including IFN-γ, IL-2, IL-17, IL-4, and IL-10 release from T lymphocytes of EAU rats, <i<in vitro</i<. Rapamycin also significantly increased TGF-β1 production but had no effect on IL-6 productionof T lymphocytes from EAU rats in vitro. Furthermore, rapamycin decreased the ratio of Th17 cells/CD4+T cells and upregulated Tregs in EAU, as detected by flow cytometry. <b<CONCLUSION:</b< Rapamycin effectively interferes with T cell mediated autoimmune uveitis by inhibiting antigen-specific T cell functions and enhancing Tregs in EAU. Rapamycin is a promising new alternative as an adjunct corticosteroid-sparing agent for treating uveitis.
abstractGer	<b<AIM:</b<To determine the effects of rapamycin on experimental autoimmune uveoretinitis (EAU) and investigate of role of rapamycin on T cell subsets in the disease. <b<METHODS</b<:EAU was induced in rats using peptides 1169 to 1191 of the interphotoreceptor binding protein (IRBP). Rapamycin (0.2 mg/kg/d) was administrated by intraperitoneal injection for a consecutive 7d after immunization. Th1/Th2/Th17 cytokines, TGF-β1, and IL-6 produced by lymphocyteswere measured by ELISA, while Th17 cells and CD4+CD25+ regulatory T cells (Tregs) from rat spleen were detected by flow cytometry. <b<RESULTS:</b< Intraperitoneal treatment immediately after immunization dramatically ameliorated the clinical course of EAU. Clinical responses were associated with reduced retinal inflammatory cell infiltration and tissue destruction. Rapamycin induced suppression of Th1/Th2/Th17 cytokines, including IFN-γ, IL-2, IL-17, IL-4, and IL-10 release from T lymphocytes of EAU rats, <i<in vitro</i<. Rapamycin also significantly increased TGF-β1 production but had no effect on IL-6 productionof T lymphocytes from EAU rats in vitro. Furthermore, rapamycin decreased the ratio of Th17 cells/CD4+T cells and upregulated Tregs in EAU, as detected by flow cytometry. <b<CONCLUSION:</b< Rapamycin effectively interferes with T cell mediated autoimmune uveitis by inhibiting antigen-specific T cell functions and enhancing Tregs in EAU. Rapamycin is a promising new alternative as an adjunct corticosteroid-sparing agent for treating uveitis.
abstract_unstemmed	<b<AIM:</b<To determine the effects of rapamycin on experimental autoimmune uveoretinitis (EAU) and investigate of role of rapamycin on T cell subsets in the disease. <b<METHODS</b<:EAU was induced in rats using peptides 1169 to 1191 of the interphotoreceptor binding protein (IRBP). Rapamycin (0.2 mg/kg/d) was administrated by intraperitoneal injection for a consecutive 7d after immunization. Th1/Th2/Th17 cytokines, TGF-β1, and IL-6 produced by lymphocyteswere measured by ELISA, while Th17 cells and CD4+CD25+ regulatory T cells (Tregs) from rat spleen were detected by flow cytometry. <b<RESULTS:</b< Intraperitoneal treatment immediately after immunization dramatically ameliorated the clinical course of EAU. Clinical responses were associated with reduced retinal inflammatory cell infiltration and tissue destruction. Rapamycin induced suppression of Th1/Th2/Th17 cytokines, including IFN-γ, IL-2, IL-17, IL-4, and IL-10 release from T lymphocytes of EAU rats, <i<in vitro</i<. Rapamycin also significantly increased TGF-β1 production but had no effect on IL-6 productionof T lymphocytes from EAU rats in vitro. Furthermore, rapamycin decreased the ratio of Th17 cells/CD4+T cells and upregulated Tregs in EAU, as detected by flow cytometry. <b<CONCLUSION:</b< Rapamycin effectively interferes with T cell mediated autoimmune uveitis by inhibiting antigen-specific T cell functions and enhancing Tregs in EAU. Rapamycin is a promising new alternative as an adjunct corticosteroid-sparing agent for treating uveitis.
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title_short	Rapamycin ameliorates experimental autoimmune uveoretinitis by inhibiting Th1/Th2/Th17 cells and upregulating CD4+CD25+ Foxp3 regulatory T cells
url	https://doi.org/10.3980/j.issn.2222-3959.2015.04.03 https://doaj.org/article/e221db0710f34d949eae343182699d12 http://www.ijo.cn/en_publish/2015/4/20150403.pdf https://doaj.org/toc/2222-3959 https://doaj.org/toc/2227-4898
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author2	Guang-Da Li Xin-Jun Ren Hong Nian Xiao-Rong Li Xiao-Min Zhang
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callnumber-a	RE1-994
up_date	2024-07-03T16:04:06.434Z
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