Viral dynamics and immune responses to foot-and-mouth disease virus in African buffalo (Syncerus caffer)

Abstract Foot-and-mouth disease (FMD) is one of the most important livestock diseases restricting international trade. While African buffalo (Syncerus caffer) act as the main wildlife reservoir, viral and immune response dynamics during FMD virus acute infection have not been described before in thi...
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Autor*in:	Eva Perez-Martin [verfasserIn] Brianna Beechler [verfasserIn] Fuquan Zhang [verfasserIn] Katherine Scott [verfasserIn] Lin-Mari de Klerk-Lorist [verfasserIn] Georgina Limon [verfasserIn] Brian Dugovich [verfasserIn] Simon Gubbins [verfasserIn] Arista Botha [verfasserIn] Robyn Hetem [verfasserIn] Louis van Schalkwyk [verfasserIn] Nicholas Juleff [verfasserIn] Francois F. Maree [verfasserIn] Anna Jolles [verfasserIn] Bryan Charleston [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	SAT FMDV host-viral interaction innate immune response acute phase proteins fever

Übergeordnetes Werk:	In: Veterinary Research - BMC, 2011, 53(2022), 1, Seite 14
Übergeordnetes Werk:	volume:53 ; year:2022 ; number:1 ; pages:14

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/s13567-022-01076-3

Katalog-ID:	DOAJ035761466

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520			\|a Abstract Foot-and-mouth disease (FMD) is one of the most important livestock diseases restricting international trade. While African buffalo (Syncerus caffer) act as the main wildlife reservoir, viral and immune response dynamics during FMD virus acute infection have not been described before in this species. We used experimental needle inoculation and contact infections with three Southern African Territories serotypes to assess clinical, virological and immunological dynamics for thirty days post infection. Clinical FMD in the needle inoculated buffalo was mild and characterised by pyrexia. Despite the absence of generalised vesicles, all contact animals were readily infected with their respective serotypes within the first two to nine days after being mixed with needle challenged buffalo. Irrespective of the route of infection or serotype, there were positive associations between the viral loads in blood and the induction of host innate pro-inflammatory cytokines and acute phase proteins. Viral loads in blood and tonsil swabs were tightly correlated during the acute phase of the infection, however, viraemia significantly declined after a peak at four days post-infection (dpi), which correlated with the presence of detectable neutralising antibodies. In contrast, infectious virus was isolated in the tonsil swabs until the last sampling point (30 dpi) in most animals. The pattern of virus detection in serum and tonsil swabs was similar for all three serotypes in the direct challenged and contact challenged animals. We have demonstrated for the first time that African buffalo are indeed systemically affected by FMD virus and clinical FMD in buffalo is characterized by a transient pyrexia. Despite the lack of FMD lesions, infection of African buffalo was characterised by high viral loads in blood and oropharynx, rapid and strong host innate and adaptive immune responses and high transmissibility.
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allfields	10.1186/s13567-022-01076-3 doi (DE-627)DOAJ035761466 (DE-599)DOAJb822a936ea7347549e9e26c36ebbadf0 DE-627 ger DE-627 rakwb eng SF600-1100 Eva Perez-Martin verfasserin aut Viral dynamics and immune responses to foot-and-mouth disease virus in African buffalo (Syncerus caffer) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Foot-and-mouth disease (FMD) is one of the most important livestock diseases restricting international trade. While African buffalo (Syncerus caffer) act as the main wildlife reservoir, viral and immune response dynamics during FMD virus acute infection have not been described before in this species. We used experimental needle inoculation and contact infections with three Southern African Territories serotypes to assess clinical, virological and immunological dynamics for thirty days post infection. Clinical FMD in the needle inoculated buffalo was mild and characterised by pyrexia. Despite the absence of generalised vesicles, all contact animals were readily infected with their respective serotypes within the first two to nine days after being mixed with needle challenged buffalo. Irrespective of the route of infection or serotype, there were positive associations between the viral loads in blood and the induction of host innate pro-inflammatory cytokines and acute phase proteins. Viral loads in blood and tonsil swabs were tightly correlated during the acute phase of the infection, however, viraemia significantly declined after a peak at four days post-infection (dpi), which correlated with the presence of detectable neutralising antibodies. In contrast, infectious virus was isolated in the tonsil swabs until the last sampling point (30 dpi) in most animals. The pattern of virus detection in serum and tonsil swabs was similar for all three serotypes in the direct challenged and contact challenged animals. We have demonstrated for the first time that African buffalo are indeed systemically affected by FMD virus and clinical FMD in buffalo is characterized by a transient pyrexia. Despite the lack of FMD lesions, infection of African buffalo was characterised by high viral loads in blood and oropharynx, rapid and strong host innate and adaptive immune responses and high transmissibility. SAT FMDV host-viral interaction innate immune response acute phase proteins fever Veterinary medicine Brianna Beechler verfasserin aut Fuquan Zhang verfasserin aut Katherine Scott verfasserin aut Lin-Mari de Klerk-Lorist verfasserin aut Georgina Limon verfasserin aut Brian Dugovich verfasserin aut Simon Gubbins verfasserin aut Arista Botha verfasserin aut Robyn Hetem verfasserin aut Louis van Schalkwyk verfasserin aut Nicholas Juleff verfasserin aut Francois F. Maree verfasserin aut Anna Jolles verfasserin aut Bryan Charleston verfasserin aut In Veterinary Research BMC, 2011 53(2022), 1, Seite 14 (DE-627)312853653 (DE-600)2012391-7 12979716 nnns volume:53 year:2022 number:1 pages:14 https://doi.org/10.1186/s13567-022-01076-3 kostenfrei https://doaj.org/article/b822a936ea7347549e9e26c36ebbadf0 kostenfrei https://doi.org/10.1186/s13567-022-01076-3 kostenfrei https://doaj.org/toc/1297-9716 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 53 2022 1 14
spelling	10.1186/s13567-022-01076-3 doi (DE-627)DOAJ035761466 (DE-599)DOAJb822a936ea7347549e9e26c36ebbadf0 DE-627 ger DE-627 rakwb eng SF600-1100 Eva Perez-Martin verfasserin aut Viral dynamics and immune responses to foot-and-mouth disease virus in African buffalo (Syncerus caffer) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Foot-and-mouth disease (FMD) is one of the most important livestock diseases restricting international trade. While African buffalo (Syncerus caffer) act as the main wildlife reservoir, viral and immune response dynamics during FMD virus acute infection have not been described before in this species. We used experimental needle inoculation and contact infections with three Southern African Territories serotypes to assess clinical, virological and immunological dynamics for thirty days post infection. Clinical FMD in the needle inoculated buffalo was mild and characterised by pyrexia. Despite the absence of generalised vesicles, all contact animals were readily infected with their respective serotypes within the first two to nine days after being mixed with needle challenged buffalo. Irrespective of the route of infection or serotype, there were positive associations between the viral loads in blood and the induction of host innate pro-inflammatory cytokines and acute phase proteins. Viral loads in blood and tonsil swabs were tightly correlated during the acute phase of the infection, however, viraemia significantly declined after a peak at four days post-infection (dpi), which correlated with the presence of detectable neutralising antibodies. In contrast, infectious virus was isolated in the tonsil swabs until the last sampling point (30 dpi) in most animals. The pattern of virus detection in serum and tonsil swabs was similar for all three serotypes in the direct challenged and contact challenged animals. We have demonstrated for the first time that African buffalo are indeed systemically affected by FMD virus and clinical FMD in buffalo is characterized by a transient pyrexia. Despite the lack of FMD lesions, infection of African buffalo was characterised by high viral loads in blood and oropharynx, rapid and strong host innate and adaptive immune responses and high transmissibility. SAT FMDV host-viral interaction innate immune response acute phase proteins fever Veterinary medicine Brianna Beechler verfasserin aut Fuquan Zhang verfasserin aut Katherine Scott verfasserin aut Lin-Mari de Klerk-Lorist verfasserin aut Georgina Limon verfasserin aut Brian Dugovich verfasserin aut Simon Gubbins verfasserin aut Arista Botha verfasserin aut Robyn Hetem verfasserin aut Louis van Schalkwyk verfasserin aut Nicholas Juleff verfasserin aut Francois F. Maree verfasserin aut Anna Jolles verfasserin aut Bryan Charleston verfasserin aut In Veterinary Research BMC, 2011 53(2022), 1, Seite 14 (DE-627)312853653 (DE-600)2012391-7 12979716 nnns volume:53 year:2022 number:1 pages:14 https://doi.org/10.1186/s13567-022-01076-3 kostenfrei https://doaj.org/article/b822a936ea7347549e9e26c36ebbadf0 kostenfrei https://doi.org/10.1186/s13567-022-01076-3 kostenfrei https://doaj.org/toc/1297-9716 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 53 2022 1 14
allfields_unstemmed	10.1186/s13567-022-01076-3 doi (DE-627)DOAJ035761466 (DE-599)DOAJb822a936ea7347549e9e26c36ebbadf0 DE-627 ger DE-627 rakwb eng SF600-1100 Eva Perez-Martin verfasserin aut Viral dynamics and immune responses to foot-and-mouth disease virus in African buffalo (Syncerus caffer) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Foot-and-mouth disease (FMD) is one of the most important livestock diseases restricting international trade. While African buffalo (Syncerus caffer) act as the main wildlife reservoir, viral and immune response dynamics during FMD virus acute infection have not been described before in this species. We used experimental needle inoculation and contact infections with three Southern African Territories serotypes to assess clinical, virological and immunological dynamics for thirty days post infection. Clinical FMD in the needle inoculated buffalo was mild and characterised by pyrexia. Despite the absence of generalised vesicles, all contact animals were readily infected with their respective serotypes within the first two to nine days after being mixed with needle challenged buffalo. Irrespective of the route of infection or serotype, there were positive associations between the viral loads in blood and the induction of host innate pro-inflammatory cytokines and acute phase proteins. Viral loads in blood and tonsil swabs were tightly correlated during the acute phase of the infection, however, viraemia significantly declined after a peak at four days post-infection (dpi), which correlated with the presence of detectable neutralising antibodies. In contrast, infectious virus was isolated in the tonsil swabs until the last sampling point (30 dpi) in most animals. The pattern of virus detection in serum and tonsil swabs was similar for all three serotypes in the direct challenged and contact challenged animals. We have demonstrated for the first time that African buffalo are indeed systemically affected by FMD virus and clinical FMD in buffalo is characterized by a transient pyrexia. Despite the lack of FMD lesions, infection of African buffalo was characterised by high viral loads in blood and oropharynx, rapid and strong host innate and adaptive immune responses and high transmissibility. SAT FMDV host-viral interaction innate immune response acute phase proteins fever Veterinary medicine Brianna Beechler verfasserin aut Fuquan Zhang verfasserin aut Katherine Scott verfasserin aut Lin-Mari de Klerk-Lorist verfasserin aut Georgina Limon verfasserin aut Brian Dugovich verfasserin aut Simon Gubbins verfasserin aut Arista Botha verfasserin aut Robyn Hetem verfasserin aut Louis van Schalkwyk verfasserin aut Nicholas Juleff verfasserin aut Francois F. Maree verfasserin aut Anna Jolles verfasserin aut Bryan Charleston verfasserin aut In Veterinary Research BMC, 2011 53(2022), 1, Seite 14 (DE-627)312853653 (DE-600)2012391-7 12979716 nnns volume:53 year:2022 number:1 pages:14 https://doi.org/10.1186/s13567-022-01076-3 kostenfrei https://doaj.org/article/b822a936ea7347549e9e26c36ebbadf0 kostenfrei https://doi.org/10.1186/s13567-022-01076-3 kostenfrei https://doaj.org/toc/1297-9716 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 53 2022 1 14
allfieldsGer	10.1186/s13567-022-01076-3 doi (DE-627)DOAJ035761466 (DE-599)DOAJb822a936ea7347549e9e26c36ebbadf0 DE-627 ger DE-627 rakwb eng SF600-1100 Eva Perez-Martin verfasserin aut Viral dynamics and immune responses to foot-and-mouth disease virus in African buffalo (Syncerus caffer) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Foot-and-mouth disease (FMD) is one of the most important livestock diseases restricting international trade. While African buffalo (Syncerus caffer) act as the main wildlife reservoir, viral and immune response dynamics during FMD virus acute infection have not been described before in this species. We used experimental needle inoculation and contact infections with three Southern African Territories serotypes to assess clinical, virological and immunological dynamics for thirty days post infection. Clinical FMD in the needle inoculated buffalo was mild and characterised by pyrexia. Despite the absence of generalised vesicles, all contact animals were readily infected with their respective serotypes within the first two to nine days after being mixed with needle challenged buffalo. Irrespective of the route of infection or serotype, there were positive associations between the viral loads in blood and the induction of host innate pro-inflammatory cytokines and acute phase proteins. Viral loads in blood and tonsil swabs were tightly correlated during the acute phase of the infection, however, viraemia significantly declined after a peak at four days post-infection (dpi), which correlated with the presence of detectable neutralising antibodies. In contrast, infectious virus was isolated in the tonsil swabs until the last sampling point (30 dpi) in most animals. The pattern of virus detection in serum and tonsil swabs was similar for all three serotypes in the direct challenged and contact challenged animals. We have demonstrated for the first time that African buffalo are indeed systemically affected by FMD virus and clinical FMD in buffalo is characterized by a transient pyrexia. Despite the lack of FMD lesions, infection of African buffalo was characterised by high viral loads in blood and oropharynx, rapid and strong host innate and adaptive immune responses and high transmissibility. SAT FMDV host-viral interaction innate immune response acute phase proteins fever Veterinary medicine Brianna Beechler verfasserin aut Fuquan Zhang verfasserin aut Katherine Scott verfasserin aut Lin-Mari de Klerk-Lorist verfasserin aut Georgina Limon verfasserin aut Brian Dugovich verfasserin aut Simon Gubbins verfasserin aut Arista Botha verfasserin aut Robyn Hetem verfasserin aut Louis van Schalkwyk verfasserin aut Nicholas Juleff verfasserin aut Francois F. Maree verfasserin aut Anna Jolles verfasserin aut Bryan Charleston verfasserin aut In Veterinary Research BMC, 2011 53(2022), 1, Seite 14 (DE-627)312853653 (DE-600)2012391-7 12979716 nnns volume:53 year:2022 number:1 pages:14 https://doi.org/10.1186/s13567-022-01076-3 kostenfrei https://doaj.org/article/b822a936ea7347549e9e26c36ebbadf0 kostenfrei https://doi.org/10.1186/s13567-022-01076-3 kostenfrei https://doaj.org/toc/1297-9716 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 53 2022 1 14
allfieldsSound	10.1186/s13567-022-01076-3 doi (DE-627)DOAJ035761466 (DE-599)DOAJb822a936ea7347549e9e26c36ebbadf0 DE-627 ger DE-627 rakwb eng SF600-1100 Eva Perez-Martin verfasserin aut Viral dynamics and immune responses to foot-and-mouth disease virus in African buffalo (Syncerus caffer) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Foot-and-mouth disease (FMD) is one of the most important livestock diseases restricting international trade. While African buffalo (Syncerus caffer) act as the main wildlife reservoir, viral and immune response dynamics during FMD virus acute infection have not been described before in this species. We used experimental needle inoculation and contact infections with three Southern African Territories serotypes to assess clinical, virological and immunological dynamics for thirty days post infection. Clinical FMD in the needle inoculated buffalo was mild and characterised by pyrexia. Despite the absence of generalised vesicles, all contact animals were readily infected with their respective serotypes within the first two to nine days after being mixed with needle challenged buffalo. Irrespective of the route of infection or serotype, there were positive associations between the viral loads in blood and the induction of host innate pro-inflammatory cytokines and acute phase proteins. Viral loads in blood and tonsil swabs were tightly correlated during the acute phase of the infection, however, viraemia significantly declined after a peak at four days post-infection (dpi), which correlated with the presence of detectable neutralising antibodies. In contrast, infectious virus was isolated in the tonsil swabs until the last sampling point (30 dpi) in most animals. The pattern of virus detection in serum and tonsil swabs was similar for all three serotypes in the direct challenged and contact challenged animals. We have demonstrated for the first time that African buffalo are indeed systemically affected by FMD virus and clinical FMD in buffalo is characterized by a transient pyrexia. Despite the lack of FMD lesions, infection of African buffalo was characterised by high viral loads in blood and oropharynx, rapid and strong host innate and adaptive immune responses and high transmissibility. SAT FMDV host-viral interaction innate immune response acute phase proteins fever Veterinary medicine Brianna Beechler verfasserin aut Fuquan Zhang verfasserin aut Katherine Scott verfasserin aut Lin-Mari de Klerk-Lorist verfasserin aut Georgina Limon verfasserin aut Brian Dugovich verfasserin aut Simon Gubbins verfasserin aut Arista Botha verfasserin aut Robyn Hetem verfasserin aut Louis van Schalkwyk verfasserin aut Nicholas Juleff verfasserin aut Francois F. Maree verfasserin aut Anna Jolles verfasserin aut Bryan Charleston verfasserin aut In Veterinary Research BMC, 2011 53(2022), 1, Seite 14 (DE-627)312853653 (DE-600)2012391-7 12979716 nnns volume:53 year:2022 number:1 pages:14 https://doi.org/10.1186/s13567-022-01076-3 kostenfrei https://doaj.org/article/b822a936ea7347549e9e26c36ebbadf0 kostenfrei https://doi.org/10.1186/s13567-022-01076-3 kostenfrei https://doaj.org/toc/1297-9716 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 53 2022 1 14
language	English
source	In Veterinary Research 53(2022), 1, Seite 14 volume:53 year:2022 number:1 pages:14
sourceStr	In Veterinary Research 53(2022), 1, Seite 14 volume:53 year:2022 number:1 pages:14
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	SAT FMDV host-viral interaction innate immune response acute phase proteins fever Veterinary medicine
isfreeaccess_bool	true
container_title	Veterinary Research
authorswithroles_txt_mv	Eva Perez-Martin @@aut@@ Brianna Beechler @@aut@@ Fuquan Zhang @@aut@@ Katherine Scott @@aut@@ Lin-Mari de Klerk-Lorist @@aut@@ Georgina Limon @@aut@@ Brian Dugovich @@aut@@ Simon Gubbins @@aut@@ Arista Botha @@aut@@ Robyn Hetem @@aut@@ Louis van Schalkwyk @@aut@@ Nicholas Juleff @@aut@@ Francois F. Maree @@aut@@ Anna Jolles @@aut@@ Bryan Charleston @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	312853653
id	DOAJ035761466
language_de	englisch
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callnumber-first	S - Agriculture
author	Eva Perez-Martin
spellingShingle	Eva Perez-Martin misc SF600-1100 misc SAT misc FMDV misc host-viral interaction misc innate immune response misc acute phase proteins misc fever misc Veterinary medicine Viral dynamics and immune responses to foot-and-mouth disease virus in African buffalo (Syncerus caffer)
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topic_title	SF600-1100 Viral dynamics and immune responses to foot-and-mouth disease virus in African buffalo (Syncerus caffer) SAT FMDV host-viral interaction innate immune response acute phase proteins fever
topic	misc SF600-1100 misc SAT misc FMDV misc host-viral interaction misc innate immune response misc acute phase proteins misc fever misc Veterinary medicine
topic_unstemmed	misc SF600-1100 misc SAT misc FMDV misc host-viral interaction misc innate immune response misc acute phase proteins misc fever misc Veterinary medicine
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title	Viral dynamics and immune responses to foot-and-mouth disease virus in African buffalo (Syncerus caffer)
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title_full	Viral dynamics and immune responses to foot-and-mouth disease virus in African buffalo (Syncerus caffer)
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author_browse	Eva Perez-Martin Brianna Beechler Fuquan Zhang Katherine Scott Lin-Mari de Klerk-Lorist Georgina Limon Brian Dugovich Simon Gubbins Arista Botha Robyn Hetem Louis van Schalkwyk Nicholas Juleff Francois F. Maree Anna Jolles Bryan Charleston
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title_sort	viral dynamics and immune responses to foot-and-mouth disease virus in african buffalo (syncerus caffer)
callnumber	SF600-1100
title_auth	Viral dynamics and immune responses to foot-and-mouth disease virus in African buffalo (Syncerus caffer)
abstract	Abstract Foot-and-mouth disease (FMD) is one of the most important livestock diseases restricting international trade. While African buffalo (Syncerus caffer) act as the main wildlife reservoir, viral and immune response dynamics during FMD virus acute infection have not been described before in this species. We used experimental needle inoculation and contact infections with three Southern African Territories serotypes to assess clinical, virological and immunological dynamics for thirty days post infection. Clinical FMD in the needle inoculated buffalo was mild and characterised by pyrexia. Despite the absence of generalised vesicles, all contact animals were readily infected with their respective serotypes within the first two to nine days after being mixed with needle challenged buffalo. Irrespective of the route of infection or serotype, there were positive associations between the viral loads in blood and the induction of host innate pro-inflammatory cytokines and acute phase proteins. Viral loads in blood and tonsil swabs were tightly correlated during the acute phase of the infection, however, viraemia significantly declined after a peak at four days post-infection (dpi), which correlated with the presence of detectable neutralising antibodies. In contrast, infectious virus was isolated in the tonsil swabs until the last sampling point (30 dpi) in most animals. The pattern of virus detection in serum and tonsil swabs was similar for all three serotypes in the direct challenged and contact challenged animals. We have demonstrated for the first time that African buffalo are indeed systemically affected by FMD virus and clinical FMD in buffalo is characterized by a transient pyrexia. Despite the lack of FMD lesions, infection of African buffalo was characterised by high viral loads in blood and oropharynx, rapid and strong host innate and adaptive immune responses and high transmissibility.
abstractGer	Abstract Foot-and-mouth disease (FMD) is one of the most important livestock diseases restricting international trade. While African buffalo (Syncerus caffer) act as the main wildlife reservoir, viral and immune response dynamics during FMD virus acute infection have not been described before in this species. We used experimental needle inoculation and contact infections with three Southern African Territories serotypes to assess clinical, virological and immunological dynamics for thirty days post infection. Clinical FMD in the needle inoculated buffalo was mild and characterised by pyrexia. Despite the absence of generalised vesicles, all contact animals were readily infected with their respective serotypes within the first two to nine days after being mixed with needle challenged buffalo. Irrespective of the route of infection or serotype, there were positive associations between the viral loads in blood and the induction of host innate pro-inflammatory cytokines and acute phase proteins. Viral loads in blood and tonsil swabs were tightly correlated during the acute phase of the infection, however, viraemia significantly declined after a peak at four days post-infection (dpi), which correlated with the presence of detectable neutralising antibodies. In contrast, infectious virus was isolated in the tonsil swabs until the last sampling point (30 dpi) in most animals. The pattern of virus detection in serum and tonsil swabs was similar for all three serotypes in the direct challenged and contact challenged animals. We have demonstrated for the first time that African buffalo are indeed systemically affected by FMD virus and clinical FMD in buffalo is characterized by a transient pyrexia. Despite the lack of FMD lesions, infection of African buffalo was characterised by high viral loads in blood and oropharynx, rapid and strong host innate and adaptive immune responses and high transmissibility.
abstract_unstemmed	Abstract Foot-and-mouth disease (FMD) is one of the most important livestock diseases restricting international trade. While African buffalo (Syncerus caffer) act as the main wildlife reservoir, viral and immune response dynamics during FMD virus acute infection have not been described before in this species. We used experimental needle inoculation and contact infections with three Southern African Territories serotypes to assess clinical, virological and immunological dynamics for thirty days post infection. Clinical FMD in the needle inoculated buffalo was mild and characterised by pyrexia. Despite the absence of generalised vesicles, all contact animals were readily infected with their respective serotypes within the first two to nine days after being mixed with needle challenged buffalo. Irrespective of the route of infection or serotype, there were positive associations between the viral loads in blood and the induction of host innate pro-inflammatory cytokines and acute phase proteins. Viral loads in blood and tonsil swabs were tightly correlated during the acute phase of the infection, however, viraemia significantly declined after a peak at four days post-infection (dpi), which correlated with the presence of detectable neutralising antibodies. In contrast, infectious virus was isolated in the tonsil swabs until the last sampling point (30 dpi) in most animals. The pattern of virus detection in serum and tonsil swabs was similar for all three serotypes in the direct challenged and contact challenged animals. We have demonstrated for the first time that African buffalo are indeed systemically affected by FMD virus and clinical FMD in buffalo is characterized by a transient pyrexia. Despite the lack of FMD lesions, infection of African buffalo was characterised by high viral loads in blood and oropharynx, rapid and strong host innate and adaptive immune responses and high transmissibility.
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title_short	Viral dynamics and immune responses to foot-and-mouth disease virus in African buffalo (Syncerus caffer)
url	https://doi.org/10.1186/s13567-022-01076-3 https://doaj.org/article/b822a936ea7347549e9e26c36ebbadf0 https://doaj.org/toc/1297-9716
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Viral dynamics and immune responses to foot-and-mouth disease virus in African buffalo (Syncerus caffer)

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