Improving the Vanillin-Sulphuric Acid Method for Quantifying Total Saponins

The colorimetric assay used for saponin quantification in plant extracts is subject to interference by common solvents used to extract the saponins from plant materials. Therefore, the degree of interference of ten common solvents was investigated. It was found that the presence of acetone, methanol...
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Autor*in:	Anh V. Le [verfasserIn] Sophie E. Parks [verfasserIn] Minh H. Nguyen [verfasserIn] Paul D. Roach [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	saponin aescin vanillin-sulphuric acid assay spectrophotometry colorimetric assay solvents interference

Übergeordnetes Werk:	In: Technologies - MDPI AG, 2014, 6(2018), 3, p 84
Übergeordnetes Werk:	volume:6 ; year:2018 ; number:3, p 84

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/technologies6030084

Katalog-ID:	DOAJ036167088

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spelling	10.3390/technologies6030084 doi (DE-627)DOAJ036167088 (DE-599)DOAJ65fe916cbbb54d3eac3215601573f4fe DE-627 ger DE-627 rakwb eng Anh V. Le verfasserin aut Improving the Vanillin-Sulphuric Acid Method for Quantifying Total Saponins 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The colorimetric assay used for saponin quantification in plant extracts is subject to interference by common solvents used to extract the saponins from plant materials. Therefore, the degree of interference of ten common solvents was investigated. It was found that the presence of acetone, methanol and n-butanol in the reaction solution caused an intense darkening of the reaction solution in the absence of saponins, which likely could lead to erroneous saponin content values. Using aescin to construct standard curves with different solvents—such as water, ethanol, and methanol— also showed significant differences in the standard curves obtained, which led to different values when they were applied to quantify the saponin content of an ethanol extract from dried and powdered Gac (Momordica cochinchinensis Spreng) seed kernels. To improve the method, a solvent evaporation step was added prior to the colorisation reaction to prevent undesired solvent interference during the reaction step. Using this modified protocol for the aescin standard curve and the Gac seed kernel extract eliminated any solvent interference. Thus, this improved protocol is recommended for the quantification of the saponin content of plant extracts irrespective of which extraction solvent is used. saponin aescin vanillin-sulphuric acid assay spectrophotometry colorimetric assay solvents interference Technology T Sophie E. Parks verfasserin aut Minh H. Nguyen verfasserin aut Paul D. Roach verfasserin aut In Technologies MDPI AG, 2014 6(2018), 3, p 84 (DE-627)736557288 (DE-600)2703026-X 22277080 nnns volume:6 year:2018 number:3, p 84 https://doi.org/10.3390/technologies6030084 kostenfrei https://doaj.org/article/65fe916cbbb54d3eac3215601573f4fe kostenfrei http://www.mdpi.com/2227-7080/6/3/84 kostenfrei https://doaj.org/toc/2227-7080 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2018 3, p 84
allfields_unstemmed	10.3390/technologies6030084 doi (DE-627)DOAJ036167088 (DE-599)DOAJ65fe916cbbb54d3eac3215601573f4fe DE-627 ger DE-627 rakwb eng Anh V. Le verfasserin aut Improving the Vanillin-Sulphuric Acid Method for Quantifying Total Saponins 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The colorimetric assay used for saponin quantification in plant extracts is subject to interference by common solvents used to extract the saponins from plant materials. Therefore, the degree of interference of ten common solvents was investigated. It was found that the presence of acetone, methanol and n-butanol in the reaction solution caused an intense darkening of the reaction solution in the absence of saponins, which likely could lead to erroneous saponin content values. Using aescin to construct standard curves with different solvents—such as water, ethanol, and methanol— also showed significant differences in the standard curves obtained, which led to different values when they were applied to quantify the saponin content of an ethanol extract from dried and powdered Gac (Momordica cochinchinensis Spreng) seed kernels. To improve the method, a solvent evaporation step was added prior to the colorisation reaction to prevent undesired solvent interference during the reaction step. Using this modified protocol for the aescin standard curve and the Gac seed kernel extract eliminated any solvent interference. Thus, this improved protocol is recommended for the quantification of the saponin content of plant extracts irrespective of which extraction solvent is used. saponin aescin vanillin-sulphuric acid assay spectrophotometry colorimetric assay solvents interference Technology T Sophie E. Parks verfasserin aut Minh H. Nguyen verfasserin aut Paul D. Roach verfasserin aut In Technologies MDPI AG, 2014 6(2018), 3, p 84 (DE-627)736557288 (DE-600)2703026-X 22277080 nnns volume:6 year:2018 number:3, p 84 https://doi.org/10.3390/technologies6030084 kostenfrei https://doaj.org/article/65fe916cbbb54d3eac3215601573f4fe kostenfrei http://www.mdpi.com/2227-7080/6/3/84 kostenfrei https://doaj.org/toc/2227-7080 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2018 3, p 84
allfieldsGer	10.3390/technologies6030084 doi (DE-627)DOAJ036167088 (DE-599)DOAJ65fe916cbbb54d3eac3215601573f4fe DE-627 ger DE-627 rakwb eng Anh V. Le verfasserin aut Improving the Vanillin-Sulphuric Acid Method for Quantifying Total Saponins 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The colorimetric assay used for saponin quantification in plant extracts is subject to interference by common solvents used to extract the saponins from plant materials. Therefore, the degree of interference of ten common solvents was investigated. It was found that the presence of acetone, methanol and n-butanol in the reaction solution caused an intense darkening of the reaction solution in the absence of saponins, which likely could lead to erroneous saponin content values. Using aescin to construct standard curves with different solvents—such as water, ethanol, and methanol— also showed significant differences in the standard curves obtained, which led to different values when they were applied to quantify the saponin content of an ethanol extract from dried and powdered Gac (Momordica cochinchinensis Spreng) seed kernels. To improve the method, a solvent evaporation step was added prior to the colorisation reaction to prevent undesired solvent interference during the reaction step. Using this modified protocol for the aescin standard curve and the Gac seed kernel extract eliminated any solvent interference. Thus, this improved protocol is recommended for the quantification of the saponin content of plant extracts irrespective of which extraction solvent is used. saponin aescin vanillin-sulphuric acid assay spectrophotometry colorimetric assay solvents interference Technology T Sophie E. Parks verfasserin aut Minh H. Nguyen verfasserin aut Paul D. Roach verfasserin aut In Technologies MDPI AG, 2014 6(2018), 3, p 84 (DE-627)736557288 (DE-600)2703026-X 22277080 nnns volume:6 year:2018 number:3, p 84 https://doi.org/10.3390/technologies6030084 kostenfrei https://doaj.org/article/65fe916cbbb54d3eac3215601573f4fe kostenfrei http://www.mdpi.com/2227-7080/6/3/84 kostenfrei https://doaj.org/toc/2227-7080 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2018 3, p 84
allfieldsSound	10.3390/technologies6030084 doi (DE-627)DOAJ036167088 (DE-599)DOAJ65fe916cbbb54d3eac3215601573f4fe DE-627 ger DE-627 rakwb eng Anh V. Le verfasserin aut Improving the Vanillin-Sulphuric Acid Method for Quantifying Total Saponins 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The colorimetric assay used for saponin quantification in plant extracts is subject to interference by common solvents used to extract the saponins from plant materials. Therefore, the degree of interference of ten common solvents was investigated. It was found that the presence of acetone, methanol and n-butanol in the reaction solution caused an intense darkening of the reaction solution in the absence of saponins, which likely could lead to erroneous saponin content values. Using aescin to construct standard curves with different solvents—such as water, ethanol, and methanol— also showed significant differences in the standard curves obtained, which led to different values when they were applied to quantify the saponin content of an ethanol extract from dried and powdered Gac (Momordica cochinchinensis Spreng) seed kernels. To improve the method, a solvent evaporation step was added prior to the colorisation reaction to prevent undesired solvent interference during the reaction step. Using this modified protocol for the aescin standard curve and the Gac seed kernel extract eliminated any solvent interference. Thus, this improved protocol is recommended for the quantification of the saponin content of plant extracts irrespective of which extraction solvent is used. saponin aescin vanillin-sulphuric acid assay spectrophotometry colorimetric assay solvents interference Technology T Sophie E. Parks verfasserin aut Minh H. Nguyen verfasserin aut Paul D. Roach verfasserin aut In Technologies MDPI AG, 2014 6(2018), 3, p 84 (DE-627)736557288 (DE-600)2703026-X 22277080 nnns volume:6 year:2018 number:3, p 84 https://doi.org/10.3390/technologies6030084 kostenfrei https://doaj.org/article/65fe916cbbb54d3eac3215601573f4fe kostenfrei http://www.mdpi.com/2227-7080/6/3/84 kostenfrei https://doaj.org/toc/2227-7080 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2018 3, p 84
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author	Anh V. Le
spellingShingle	Anh V. Le misc saponin misc aescin misc vanillin-sulphuric acid assay misc spectrophotometry misc colorimetric assay misc solvents misc interference misc Technology misc T Improving the Vanillin-Sulphuric Acid Method for Quantifying Total Saponins
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topic_title	Improving the Vanillin-Sulphuric Acid Method for Quantifying Total Saponins saponin aescin vanillin-sulphuric acid assay spectrophotometry colorimetric assay solvents interference
topic	misc saponin misc aescin misc vanillin-sulphuric acid assay misc spectrophotometry misc colorimetric assay misc solvents misc interference misc Technology misc T
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title	Improving the Vanillin-Sulphuric Acid Method for Quantifying Total Saponins
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title_sort	improving the vanillin-sulphuric acid method for quantifying total saponins
title_auth	Improving the Vanillin-Sulphuric Acid Method for Quantifying Total Saponins
abstract	The colorimetric assay used for saponin quantification in plant extracts is subject to interference by common solvents used to extract the saponins from plant materials. Therefore, the degree of interference of ten common solvents was investigated. It was found that the presence of acetone, methanol and n-butanol in the reaction solution caused an intense darkening of the reaction solution in the absence of saponins, which likely could lead to erroneous saponin content values. Using aescin to construct standard curves with different solvents—such as water, ethanol, and methanol— also showed significant differences in the standard curves obtained, which led to different values when they were applied to quantify the saponin content of an ethanol extract from dried and powdered Gac (Momordica cochinchinensis Spreng) seed kernels. To improve the method, a solvent evaporation step was added prior to the colorisation reaction to prevent undesired solvent interference during the reaction step. Using this modified protocol for the aescin standard curve and the Gac seed kernel extract eliminated any solvent interference. Thus, this improved protocol is recommended for the quantification of the saponin content of plant extracts irrespective of which extraction solvent is used.
abstractGer	The colorimetric assay used for saponin quantification in plant extracts is subject to interference by common solvents used to extract the saponins from plant materials. Therefore, the degree of interference of ten common solvents was investigated. It was found that the presence of acetone, methanol and n-butanol in the reaction solution caused an intense darkening of the reaction solution in the absence of saponins, which likely could lead to erroneous saponin content values. Using aescin to construct standard curves with different solvents—such as water, ethanol, and methanol— also showed significant differences in the standard curves obtained, which led to different values when they were applied to quantify the saponin content of an ethanol extract from dried and powdered Gac (Momordica cochinchinensis Spreng) seed kernels. To improve the method, a solvent evaporation step was added prior to the colorisation reaction to prevent undesired solvent interference during the reaction step. Using this modified protocol for the aescin standard curve and the Gac seed kernel extract eliminated any solvent interference. Thus, this improved protocol is recommended for the quantification of the saponin content of plant extracts irrespective of which extraction solvent is used.
abstract_unstemmed	The colorimetric assay used for saponin quantification in plant extracts is subject to interference by common solvents used to extract the saponins from plant materials. Therefore, the degree of interference of ten common solvents was investigated. It was found that the presence of acetone, methanol and n-butanol in the reaction solution caused an intense darkening of the reaction solution in the absence of saponins, which likely could lead to erroneous saponin content values. Using aescin to construct standard curves with different solvents—such as water, ethanol, and methanol— also showed significant differences in the standard curves obtained, which led to different values when they were applied to quantify the saponin content of an ethanol extract from dried and powdered Gac (Momordica cochinchinensis Spreng) seed kernels. To improve the method, a solvent evaporation step was added prior to the colorisation reaction to prevent undesired solvent interference during the reaction step. Using this modified protocol for the aescin standard curve and the Gac seed kernel extract eliminated any solvent interference. Thus, this improved protocol is recommended for the quantification of the saponin content of plant extracts irrespective of which extraction solvent is used.
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title_short	Improving the Vanillin-Sulphuric Acid Method for Quantifying Total Saponins
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Improving the Vanillin-Sulphuric Acid Method for Quantifying Total Saponins

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